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1.
Food Chem ; 348: 129035, 2021 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-33524690

RESUMO

In order to improve its aqueous solubility and emulsifying function, zein was partially hydrolyzed by trypsin and conjugated to chitosan oligosaccharide lactate by transglutaminase. Hydrolysis and covalent linkage to chitosan oligosaccharide was confirmed by free amine content, gel electrophoresis, and infrared spectroscopy. Enzymatic glycosylation was optimized at pH 6, 44 °C, and 4 h to bind approximately 95% of the free amines in the hydrolysates to chitosan oligosaccharide. Hydrolysis and conjugation increased solubility of zein by 47.60% and 72.93%. Hydrolysis and conjugation also decreased surface hydrophobicity by more than 20% and more than doubled emulsifying activity index, emulsion stability index, and foaming capacity. This enzymatic modification has potential to be applied to improve functional properties of other prolamins.


Assuntos
Quitosana/química , Oligossacarídeos/química , Zeína/química , Glicosilação , Hidrólise , Interações Hidrofóbicas e Hidrofílicas , Oligossacarídeos/metabolismo , Solubilidade , Transglutaminases/metabolismo , Água/química
2.
Food Chem ; 349: 129112, 2021 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-33581437

RESUMO

Zein/low-acyl gellan gum (GG) composite particles (ZGPs) were fabricated to stabilize Pickering emulsions (termed "ZGPEs"). The wettability of ZGPs was manipulated simply by adjusting the concentration of GG. The effects of GG concentration, oil fraction and pH on ZGPEs were systematically evaluated by confocal laser scanning microscopy (CLSM), cryo-scanning electron microscopy (cryo-SEM), dynamic light scattering technique, stimulated emission depletion (STED) nanoscopy and rheology. The results showed that ZGPEs exhibited robust colloidal properties and distinct advantage over other previously reported zein-polysaccharide-based Pickering emulsions. CLSM, STED and cryo-SEM analyses revealed that the network structures formed by GG and ZGPs at the continuous phase and oil-water interface were the main contributors to the emulsion's characteristics. This study provides insights into the fabrication of food-grade Pickering emulsions with distinct characteristics that impart favorable properties to various foods and bioactive delivery systems.


Assuntos
Nanoestruturas/química , Polissacarídeos Bacterianos/química , Zeína/química , Emulsões , Géis , Tamanho da Partícula , Reologia , Molhabilidade
3.
Food Chem ; 351: 129286, 2021 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-33640771

RESUMO

Hidden mycotoxins have been reported to be "protected" by macromolecular substances to escape routine determination, but release to free mycotoxins under gastrointestinal conditions. Nowadays, the hidden zearalenone (ZEN) that binding with macromolecular zein has been found in maize. However, the binding mechanism of ZEN with zein in maize has not been clarified. In this study, the formation of ZEN-zein complex was investigated applying ultrafiltration, multi-spectroscopic and molecular modeling techniques. The steady-state and transient fluorescence analysis suggested the ZEN could interact with zein to form the complex driven by hydrophobic force and hydrogen bonds, which is in accordance with the molecular modeling studies. The conformational changes of zein induced by binding with ZEN were revealed by Fourier transform infrared spectroscopy (FTIR) and circular dichroism (CD). Elucidating the binding mechanism between zein and ZEN could help the development of detecting hidden ZEN and guarantee the safety of maize products.


Assuntos
Zea mays/química , Zearalenona/química , Sítios de Ligação , Dicroísmo Circular , Ligação de Hidrogênio , Interações Hidrofóbicas e Hidrofílicas , Simulação de Acoplamento Molecular , Espectroscopia de Infravermelho com Transformada de Fourier , Ultrafiltração , Zea mays/metabolismo , Zearalenona/metabolismo , Zeína/química , Zeína/metabolismo
4.
Carbohydr Polym ; 256: 117515, 2021 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-33483036

RESUMO

Although the stabilization of Pickering emulsions based on the interaction of pectin and zein is known to be effective, the effects of the degree of methylation (DM) of pectin on the stability of Pickering emulsions remains unclear. In the present study, pectin/zein particle dispersions (PZDs) were fabricated by anti-solvent precipitation based on different DM values of pectin (13.70 %-71.42 %). Differential Scanning Calorimetry (DSC) curves and Fourier transform infrared spectroscopy (FTIR) confirmed the methyl ester carbonyl and carbonyl groups significantly affected the formation of hydrogen bonds, which consequently resulting in the secondary conformation of PZDs as reflected by circular dichroism (CD). The PZDs fabricated by pectin with DM values higher than 35.50 % formation of the network between the barriers of the droplets, showed high stability against pH (3.0-9.0) and Ca2+ (60-300 mM) at an oil fraction (φ) of 0.60. Furthermore, the Pickering emulsions showed good lycopene protective effects.


Assuntos
Licopeno/química , Pectinas/química , Zeína/química , Varredura Diferencial de Calorimetria , Estabilidade de Medicamentos , Emulsões , Ligação de Hidrogênio , Concentração de Íons de Hidrogênio , Metilação , Conformação Molecular , Peso Molecular , Tamanho da Partícula , Carbonilação Proteica , Reologia , Espectroscopia de Infravermelho com Transformada de Fourier , Eletricidade Estática
5.
Int J Mol Sci ; 22(3)2021 Jan 24.
Artigo em Inglês | MEDLINE | ID: mdl-33498911

RESUMO

Artemether, an artemisinin derivative, is used in the management of life-threatening severe malaria. This study aimed to develop an intravenous dosage form of artemether using nanotechnology. Artemether-loaded zein nanoparticles were prepared by modified antisolvent precipitation using sodium caseinate as a stabilizer. Subsequently, the physicochemical properties of the nanoparticles were characterized; the in vitro hemolytic property was examined with red blood cells, while the pharmacokinetic profile was evaluated in Sprague-Dawley rats after intravenous administration. The artemether-loaded zein nanoparticles were found to display good encapsulation efficiency, excellent physical stability and offer an in vitro extended-release property. Interestingly, encapsulation of artemether into zein nanoparticles substantially suppressed hemolysis, a common clinical phenomenon occurring after artemisinin-based antimalarial therapy. Upon intravenous administration, artemether-loaded zein nanoparticles extended the mean residence time of artemether by ~80% in comparison to the free artemether formulation (82.9 ± 15.2 versus 45.6 ± 16.4 min, p < 0.01), suggesting that the nanoparticles may prolong the therapeutic duration and reduce the dosing frequency in a clinical setting. In conclusion, intravenous delivery of artemether by artemether-loaded zein nanoparticles appears to be a promising therapeutic option for severe malaria.


Assuntos
Artemeter/administração & dosagem , Artemeter/farmacocinética , Malária/tratamento farmacológico , Nanopartículas/química , Zeína/química , Administração Intravenosa , Animais , Antimaláricos/administração & dosagem , Antimaláricos/uso terapêutico , Artemeter/uso terapêutico , Caseínas/química , Preparações de Ação Retardada , Ratos , Ratos Sprague-Dawley
6.
Food Chem ; 338: 128010, 2021 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-32932084

RESUMO

Potential improvements to the physical properties of brittle, self-assembled zein networks through microbial transglutaminase crosslinking were investigated. The formation of crosslinked heteropolymers was also explored with networks containing zein and either soy or pea protein isolates as supplemented lysine sources. The observed SDS-PAGE bands did not show any evidence of zein crosslinking. Soy and pea isolates underwent extensive crosslinking on their own, but heteropolymers were not observed in multiprotein networks with zein. Despite the lack of crosslinking observed, rheological and textural analysis revealed that the enzymatic treatment of zein produced a weaker, more brittle structure. With no significant changes in secondary structure, determined through FTIR, the observed behaviour was primarily attributed to glutamine deamidation by microbial transglutaminase in the absence of sufficient lysine through changes to the hydrophobicity of the protein such that non-covalent bonding within network was modified.


Assuntos
Transglutaminases/metabolismo , Zeína/química , Reagentes para Ligações Cruzadas/química , Eletroforese em Gel de Poliacrilamida , Interações Hidrofóbicas e Hidrofílicas , Lisina/química , Ervilhas/química , Estrutura Secundária de Proteína , Reologia , Proteínas de Soja/química , Proteínas de Soja/metabolismo , Espectroscopia de Infravermelho com Transformada de Fourier , Transglutaminases/química , Zeína/metabolismo
7.
Food Chem ; 340: 127893, 2021 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-32889202

RESUMO

In this work, zein/carboxymethyl dextrin nanoparticles were successfully fabricated at different zein to carboxymethyl dextrin (CMD) mass ratios. Zein/CMD nanoparticles with the negative charge and the smallest size (212 nm) were formed when the mass ratio of zein to CMD was 2:1, exhibiting improved encapsulation efficiency of curcumin (85.5%). Electrostatic interactions, hydrogen bonding and hydrophobic interactions were main driven forces for nanoparticles formulation and curcumin encapsulation. Fourier transform infrared spectroscopy determined curcumin might be partially embedded in CMD during encapsulation. The spherical structures of zein/CMD nanoparticles and curcumin-loaded zein/CMD nanoparticles were observed by transmission electron microscopy. The photothermal stability and antioxidant activity of curcumin were significantly enhanced after be loaded in zein/CMD nanoparticles. Furthermore, encapsulation of curcumin in zein/CMD nanoparticles significantly delayed the release of curcumin in simulated gastrointestinal fluids. These results indicated that zein/CMD nanoparticles could be effective encapsulating materials for bioactive compounds in food industry.


Assuntos
Antioxidantes/química , Curcumina/farmacocinética , Dextrinas/química , Nanopartículas/química , Zeína/química , Antioxidantes/farmacologia , Curcumina/administração & dosagem , Curcumina/química , Preparações de Ação Retardada , Estabilidade de Medicamentos , Ligação de Hidrogênio , Interações Hidrofóbicas e Hidrofílicas , Microscopia Eletrônica de Transmissão , Nanopartículas/administração & dosagem , Tamanho da Partícula , Espectrometria de Fluorescência , Espectroscopia de Infravermelho com Transformada de Fourier , Eletricidade Estática , Difração de Raios X
8.
Carbohydr Polym ; 251: 117035, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-33142593

RESUMO

A biomimetic-based approaches, especially with artificial scaffolding, have established great potential to provide tissue regeneration capacity and an effective way to bridge the gap between host cell responses and organ demands. However, the synthesis of biomaterial is most efficient when the functional behavior involved most resembles the natural extracellular matrix. Here, a fibrous scaffold was engineered by integrating zein and chitosan (CS) in to polyurethane (PU) associated with functionalized multiwalled carbon nanotubes (fMWCNTs) as a bone cell repair material. The chitosan-based, tissue-engineered scaffold containing 0.1 mg/mL fMWCNTs shows potent synergistic results where improved biomechanical strength, hydrophilicity and antibacterial efficacy produce a scaffold akin to a truly natural extracellular matrix found in the bone cell microenvironments. The scaffold enables rapid cell-to-cell communication through a bio-interface and greatly promotes the regenerative effect of pre-osteoblast (MC3T3-E1) which is reflected in terms of cell growth, proliferation, and differentiation in our in vitro experiments. Alizarin red staining analysis, alkaline phosphatase activity, and Western blotting also confirm the nucleation of hydroxyapatite (HA) nanocrystals and the expression of osteogenic protein markers, all of which indicate the scaffold's excellent osteoinductive properties. These results suggest that this precisely engineered PU/Zein/CS-fMWCNTs fibrous scaffold possesses suitable biological behavior to act as an artificial bone extracellular matrix that will ensure bone cell regeneration while contributing numerous benefits to the field of artificial bone grafts.


Assuntos
Materiais Biocompatíveis , Regeneração Óssea , Quitosana , Osteogênese , Engenharia Tecidual , Tecidos Suporte , Zeína , Animais , Materiais Biocompatíveis/química , Diferenciação Celular , Linhagem Celular , Proliferação de Células , Microambiente Celular , Quitosana/química , Camundongos , Nanotubos de Carbono/química , Osteoblastos , Poliuretanos/química , Zeína/química
9.
Int J Nanomedicine ; 15: 7995-8018, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33116514

RESUMO

Purpose: Zein/phospholipid composite nanoparticles (CNPs) were developed as a delivery platform for gallic acid (GA), a polyphenolic compound with reported preclinical antifibrotic activities. However, the therapeutic applicability of GA is hampered owing to its low bioavailability and rapid clearance. Accordingly, we developed GA-loaded CNPs. The effect of their size, surface charge and targeting strategies was investigated and optimized, with the aim of enhancing their ability to deliver GA to the activated hepatic stellate cells (aHSCs) in order to suppress hepatic fibrosis progression. Methods: Different CNP systems were prepared and characterized with regard to their particle size, zeta potential, and GA entrapment efficiency (EE%). Also, they were statistically optimized via response surface methodology. The optimized systems were investigated with regard to their in vitro GA release, in vitro efficacy on aHSCs, and in vivo biodistribution in healthy rats. Results: The GA-loaded cationic CNPs coupled with vitamin A (GA-CACNP/VA; 192 nm) showed high GA EE% (60% w/w), highest cellular internalization via active targeting, and more selective hepatic distribution, relative to free GA solution, GA-loaded anionic, and GA-loaded cationic systems. Furthermore, GA-CACNP/VA markedly triggered the apoptosis of aHSCs, repressed collagen deposition, and inhibited HSCs' activation to a lesser extent. Conclusion: The GA-CACNP/VA was shown to be a promising candidate for specific and controlled delivery of GA to aHSCs, which may provide an effective antifibrotic therapeutic approach.


Assuntos
Portadores de Fármacos/química , Ácido Gálico/química , Células Estreladas do Fígado/metabolismo , Nanopartículas/química , Fosfolipídeos/química , Vitamina A/química , Zeína/química , Animais , Apoptose/efeitos dos fármacos , Disponibilidade Biológica , Ácido Gálico/metabolismo , Ácido Gálico/farmacocinética , Ácido Gálico/farmacologia , Células Estreladas do Fígado/citologia , Células Estreladas do Fígado/efeitos dos fármacos , Masculino , Tamanho da Partícula , Ratos , Propriedades de Superfície , Distribuição Tecidual
10.
Food Chem ; 330: 127245, 2020 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-32521399

RESUMO

Dihydromyricetin (DMY) encapsulated zein-caseinate nanoparticles (DZP) were fabricated by antisolvent method. The encapsulation and loading efficiency of DMY in DZP were 90.2% and 22.6% as determined by HPLC. DZP is spherical with particle size and ζ potential of 206.4 nm and -29.6 mV, respectively. Physicochemical characterization showed that DMY existed in amorphous form in DZP and its interaction with proteins was found. The fabrication of DZP significantly improved the stability of DMY. Besides, the diffusion rate of DMY in DZP was faster than its suspensions in both simulated gastric and intestinal fluid. The adhesion of DMY in mice gastrointestinal tract was also improved. Besides DMY itself, its methylated metabolites with further sulfation and glucuronide were identified in rat plasma by UPLC-QTOF-MS. UPLC-QqQ-MS/MS quantitative analysis showed that the oral bioavailability of DMY was 1.95 times enhanced. Besides, the concentration of DMY metabolites in plasma were all increased.


Assuntos
Caseínas/química , Flavonóis/química , Nanopartículas/química , Zeína/química , Animais , Disponibilidade Biológica , Caseínas/metabolismo , Cromatografia Líquida de Alta Pressão , Feminino , Flavonóis/metabolismo , Camundongos , Tamanho da Partícula , Ratos , Espectrometria de Massas em Tandem , Zeína/metabolismo
11.
Food Chem ; 331: 127349, 2020 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-32593041

RESUMO

Atomic force microscopy was applied to characterize the self-assembling behaviors of α-zein molecules in 70% (v/v) aqueous ethanol solution under different parameters including α-zein concentration (0.001%-0.1%, w/v), pH (2.0-8.0) and the thermal treatment (90 â„ƒ, 2-24 h). α-Zein (0.1% and 0.01%, w/v) at pH 7.0 formed globules while α-zein assemblies (0.001%, w/v) exhibited the co-existence of worm-like strings, bundles of fibers, and rod-like fibers. Heating the aqueous ethanol solutions containing 0.001% (w/v) α-zein at 90 °C and pH 4.0 converted the irregular aggregates into regular spherical particles (100-120 nm), followed by fibrils (15-50 nm) at a prolonged times (8 h). Besides, fibrils were formed after heating aqueous ethanol solutions containing α-zein (0.001%, w/v) at pH 2.0 for 8 h. A two-step mechanism was proposed to explain such findings, which involved the aggregation of α-zein molecules to form aggregates, and followed by the rearrangement of α-zein molecules to form fibrils.


Assuntos
Soja/química , Zeína/química , Etanol/química , Calefação , Concentração de Íons de Hidrogênio , Microscopia de Força Atômica , Água/química
12.
Food Chem ; 330: 127172, 2020 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-32531634

RESUMO

This study demonstrates the development of biocompatible Ag nanoparticles/Tannic acid/Zein electrospun membranes with synergistic antibacterial, catalytic and antioxidant activity. The optimal spinning concentration of zein was 32 wt%. The prepared zein electrospun membranes were immersed into tannic acid (TA) solution to investigate the effects of TA concentrations, pH, temperature and time on the loading amount of TA. Then, the TA/Zein electrospun membranes were immersed into a silver nitrate solution to reduce the AgNPs in situ. The morphology of the electrospun membranes was characterized by scanning electron microscopy (SEM). UV-visible spectrophotometer, Fourier-transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) were used to carry out the loading amount of TA and Ag nanoparticles (AgNPs). Finally, the antioxidant, antibacterial and catalytic activity of TA/Zein and AgNPs/TA/Zein electrospun membranes were studied. It was found that the AgNPs/TA/Zein electrospun membranes with different TA concentrations have certain antibacterial, antioxidation and catalytic ability, which may be of interest for the development of active packaging that could extend the shelf life of perishable foods.


Assuntos
Antibacterianos/química , Antioxidantes/química , Nanopartículas Metálicas/química , Prata/química , Soja/química , Taninos/química , Zeína/química , Antibacterianos/farmacologia , Catálise , Escherichia coli/efeitos dos fármacos , Staphylococcus aureus/efeitos dos fármacos
13.
Food Chem ; 326: 126969, 2020 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-32438229

RESUMO

Biocompatible magnetic molecularly imprinted polymers (BMMIPs) were prepared with Zein for the first time, and were used to enrich tetracycline compounds selectively. Innovative combination of BMMIPs and electrochemistry to obtain lower detection line to satisfy industrial detection demands. Using Zein as the crosslinking agent, the polymers were synthesized on the surface of Fe3O4 particles. The scanning electron microscope, transmission electron microscope and X-ray diffraction technologies were used to characterize BMMIPs. Through optimization, BMMIPs attained large adsorption capacity (236.40 mg/g) with fast kinetics (40 min) and followed the Langmuir isotherm and pseudo-second-order kinetic models. BMMIPs had good recognition ability, the selective factors of oxytetracycline, chlortetracycline, doxycycline were 4.78, 4.23, and 3.39, respectively. Excellent linearity was attained in the range of 0.025-500 µg/mL, with low detection limits and low quantitation limits of 0.025 and 0.083 µg/mL. According to our exploring, BMMIPs was ideal materials for enrichment of tetracycline in complex biological samples.


Assuntos
Materiais Biocompatíveis/química , Contaminação de Alimentos/análise , Leite/química , Impressão Molecular/métodos , Tetraciclinas/análise , Adsorção , Animais , Antibacterianos/análise , Antibacterianos/química , Antibacterianos/isolamento & purificação , Técnicas Eletroquímicas , Análise de Alimentos/métodos , Limite de Detecção , Fenômenos Magnéticos , Nanopartículas de Magnetita/química , Polímeros/química , Tetraciclina/análise , Tetraciclina/química , Tetraciclina/isolamento & purificação , Tetraciclinas/química , Tetraciclinas/isolamento & purificação , Difração de Raios X , Zeína/química
14.
J Nanobiotechnology ; 18(1): 67, 2020 Apr 28.
Artigo em Inglês | MEDLINE | ID: mdl-32345323

RESUMO

BACKGROUND: Exenatide is an insulinotropic peptide drug for type 2 diabetes treatment with low risk of hypoglycemia, and is administrated by subcutaneous injection. Oral administration is the most preferred route for lifelong treatment of diabetes, but oral delivery of peptide drug remains a significant challenge due to the absorption obstacles in gastrointestinal tract. We aimed to produce exenatide-loaded nanoparticles containing absorption enhancer, protectant and stabilizer using FDA approved inactive ingredients and easy to scale-up method, and to evaluate their long-term oral therapeutic effect in type 2 diabetes db/db mice. RESULTS: Two types of nanoparticles, named COM NPs and DIS NPs, were fabricated using anti-solvent precipitation method. In COM NPs, the exenatide was complexed with cholic acid and phosphatidylcholine to increase the exenatide loading efficiency. In both nanoparticles, zein acted as the cement and the other ingredients were embedded in zein nanoparticles by hydrophobic interaction. Casein acted as the stabilizer. The nanoparticles had excellent lyophilization, storage and re-dispersion stability. Hypromellose phthalate protected the loaded exenatide from degradation in simulated gastric fluid. Cholic acid promoted the intestinal absorption of the loaded exenatide via bile acid transporters. The exenatide loading efficiencies of COM NPs and DIS NPs were 79.7% and 53.6%, respectively. The exenatide oral pharmacological availability of COM NPs was 18.6% and DIS NPs was 13.1%. COM NPs controlled the blood glucose level of the db/db mice well and the HbA1c concentration significantly decreased to 6.8% during and after 7 weeks of once daily oral administration consecutively. Both DIS NPs and COM NPs oral groups substantially increased the insulin secretion by more than 60% and promoted the ß-cell proliferation by more than 120% after the 7-week administration. CONCLUSIONS: Both COM NPs and DIS NPs are promising systems for oral delivery of exenatide, and COM NPs are better in blood glucose level control than DIS NPs. Using prolamin to produce multifunctional nanoparticles for oral delivery of peptide drug by hydrophobic interaction is a simple and effective strategy.


Assuntos
Exenatida/farmacologia , Hipoglicemiantes/farmacologia , Células Secretoras de Insulina/efeitos dos fármacos , Nanopartículas/química , Zeína/química , Administração Oral , Animais , Glicemia/análise , Ácido Cólico/química , Diabetes Mellitus Tipo 2/tratamento farmacológico , Diabetes Mellitus Tipo 2/patologia , Portadores de Fármacos/química , Portadores de Fármacos/farmacocinética , Exenatida/administração & dosagem , Exenatida/química , Trato Gastrointestinal/química , Trato Gastrointestinal/patologia , Hemoglobina A Glicada/análise , Meia-Vida , Hipoglicemiantes/administração & dosagem , Hipoglicemiantes/química , Células Secretoras de Insulina/classificação , Células Secretoras de Insulina/metabolismo , Mucosa Intestinal/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos ICR , Nanopartículas/metabolismo , Permeabilidade/efeitos dos fármacos , Fosfatidilcolinas/química
15.
Food Chem ; 321: 126712, 2020 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-32247179

RESUMO

Zein, a class of prolamine proteins extracted from maize, is extensively used in the food and pharmaceutical industries. Characterization of its components is essential for quality control and safety evaluation. We performed in silico digestion of zein proteins using tandem combinations of different proteinases, to improve protein sequence coverage and subsequent identification by nano-LC-MS/MS analysis. Trypsin/chymotrypsin yielded the highest protein sequence coverage of up to 79.5% and increased the number of proteins from 11 to 35 compared to trypsin/Lys-C. Besides, SDS-PAGE analysis revealed 37 proteins in the zein extract, as well as the possibility of protein polymers. Also, 420 peptides originating from 71 proteins were identified, of which 116 were predicted as bioactive by in silico approach. In conclusion, in silico prediction coupled with multi-enzyme digestion can significantly improve the coverage of complex zein protein proteome, and the potential function of zein proteins and peptides need be further investigated.


Assuntos
Excipientes/química , Peptídeos/química , Zea mays/química , Zeína/química , Sequência de Aminoácidos , Cromatografia Líquida de Alta Pressão , Excipientes/metabolismo , Peptídeos/metabolismo , Proteoma/química , Proteoma/metabolismo , Espectrometria de Massas em Tandem , Zea mays/metabolismo , Zeína/metabolismo
16.
Food Chem ; 319: 126577, 2020 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-32172044

RESUMO

Many liposoluble nutrients are restricted for applications in foods due to their poor water solubility, stability and bioavailability. Here, a deamidated zein peptide with a broad molecular weight distribution and high carboxyl and hydrophobic residue contents was used to solubilize curcumin. The complex nanoparticles of curcumin and the peptide were produced through self-assembly in aqueous solutions. Fluorescence and infrared spectra revealed that hydrogen bonding and hydrophobic interactions actuated the assembly. The complex nanoparticles had a curcumin loading capacity of 31.9% and completely inhibited curcumin crystallization. The peptide effectively protected curcumin from decomposition in aqueous solutions by inhibiting the reaction between dissolved oxygen and curcumin. The nanoparticles presented excellent freeze-drying/re-dispersion stability without any lyoprotectant. The curcumin bioaccessibility of the nanoparticles was 75% and the nanoparticles exerted a significant antioxidant effect after oral administration in mice. This study indicates that the nanoparticles are potentially useful as an antioxidant additive in foods.


Assuntos
Curcumina/química , Curcumina/farmacocinética , Nanopartículas/química , Zeína/química , Administração Oral , Aminação , Animais , Antioxidantes/administração & dosagem , Antioxidantes/química , Antioxidantes/farmacocinética , Disponibilidade Biológica , Cristalização , Curcumina/administração & dosagem , Liofilização , Ligação de Hidrogênio , Hidrólise , Interações Hidrofóbicas e Hidrofílicas , Masculino , Camundongos Endogâmicos ICR , Estabilidade Proteica , Solubilidade , Espectroscopia de Infravermelho com Transformada de Fourier
17.
Food Funct ; 11(2): 1810-1825, 2020 Feb 26.
Artigo em Inglês | MEDLINE | ID: mdl-32057043

RESUMO

Anti-solvent co-precipitation (ASCP) is the most commonly used method of fabricating food-grade nanoparticles, while the impact of the blending sequence on the formation of nanoparticles lacks research. In this study, 7,8-dihydroxyflavone (7,8-DHF) loaded zein-/sophorolipid nanoparticles with two blending sequences (DHF-Z-S and DHF-Z/S) were successfully fabricated by the ASCP method and used to improve the storage stability and the in vitro bioaccessibility of 7,8-DHF. The results showed that blending sequences significantly affect the physicochemical properties of nanoparticles. DHF-Z-S nanoparticles had smaller particle size, lower polydispersity index and turbidity, and higher negative charge, entrapment efficiency and loading capacity compared to DHF-Z/S nanoparticles. Transmission electron microscopy and scanning electron microscopy revealed that DHF-Z-S and DHF-Z/S nanoparticles have core-shell spherical shape at the nanoscale and sophorolipid changed the surface morphology of zein nanoparticles. Fourier transform infrared spectroscopy and fluorescence spectrum analysis confirmed the presence of effective hydrogen bonding, electrostatic interactions and hydrophobic effects between 7,8-DHF, zein and sophorolipid and the presence of stronger hydrogen bonding and hydrophobic effects in DHF-Z-S nanoparticles. The encapsulated 7,8-DHF was in an amorphous state rather than a crystalline form as determined by X-ray diffraction analysis. Circular dichroism revealed that 7,8-DHF and sophorolipid were capable of changing the secondary structure of zein remarkably. More importantly, compared to DHF-Z/S nanoparticles, the DHF-Z-S nanoparticles possessed higher storage stability and in vitro bioaccessibility. Collectively, DHF-Z-S nanoparticles developed in this study might be a promising means of encapsulating, protecting and delivering hydrophobic nutraceuticals for applications in functional foods.


Assuntos
Flavonas , Nanopartículas/química , Ácidos Oleicos/química , Zeína/química , Composição de Medicamentos , Flavonas/química , Flavonas/farmacocinética , Interações Hidrofóbicas e Hidrofílicas , Modelos Biológicos , Concentração Osmolar , Tamanho da Partícula
18.
Food Funct ; 11(2): 1647-1660, 2020 Feb 26.
Artigo em Inglês | MEDLINE | ID: mdl-32025676

RESUMO

ß-Carotene is a lipophilic bioactive compound, providing significant health benefits. Formulation of ß-carotene-enriched functional foods is a challenge, due to its poor stability, sensitivity towards light, temperature, oxygen, and its poor water solubility which leads to low bioaccessibility and bioavailability. Targeted delivery and controlled release of bioactive compounds directly depend on the encapsulating matrix and particle size. This work reports an effective encapsulation of ß-carotene in zein matrix with glycerol as stabilizing agent. ß-Carotene was encapsulated in zein protein matrix with different core-to-wall ratios (1 : 10, 1 : 50 and 1 : 100) at micro- and nano-level, through spray drying and electrospraying techniques, respectively. A comparative evaluation of processing technique, resulting particle size and its impact on powder flow properties, dissolution, release and absorption behaviour was conducted. Results showed that up to 81% of encapsulation efficiency was achieved for the nanoencapsulated form obtained through the electrospraying technique. Nanoencapsulates showed excellent dissolution behaviour compared to microencapsulates due to reduced particle size and larger surface area. Further, under simulated in vitro gastrointestinal conditions, nanoencapsulates showed faster release than microparticles. Among the three ratios tested, nanoencapsulates at 1 : 50 were found to be optimal with ∼73% encapsulation efficiency, exhibiting faster release giving more bioaccessibility, with 1.29- and 1.36-fold higher permeability than 1 : 10 and 1 : 100 formulations, respectively. Additionally, the 1 : 50 nanoencapsulates gave ∼1.7-fold increased permeability compared to microparticles at the end of 3 h using an ex vivo everted gut sac technique. This study proves the potential of zein nanoparticles for enhanced permeability and bioavailability of ß-carotene.


Assuntos
Nanocápsulas/química , Zeína/química , beta Caroteno , Animais , Disponibilidade Biológica , Galinhas , Composição de Medicamentos , Absorção Intestinal , Jejuno/metabolismo , Tamanho da Partícula , beta Caroteno/química , beta Caroteno/farmacocinética
19.
Int J Biol Macromol ; 146: 179-192, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31899246

RESUMO

7,8-dihydroxyflavone (7,8-DHF), a tyrosine kinase B (TrkB) receptor agonist, can mimick physiological actions of brain-derived neurotrophic factor (BDNF) to attenuate neurogenic disease. However, its use as a functional food, is limited by its low-water solubility, chemical instability, and poor bioavailability. The purpose of this work is to fabricate stable 7,8-DHF loaded zein/lactoferrin (LF) composite nanoparticles (zein/LF-DHF) to overcome these challenges. Results showed that mean particle size of zein/LF nanoparticles was about 74 nm with low polydispersity index (<0.200) and turbidity (<0.300) values. Zein/LF nanoparticles had good stability against pH (3.0-9.0), ionic strengths (0-500 mM NaCl at neutral pH) and long-term storage. Zein/LF nanoparticles showed spherical structures formed by hydrogen bonding and hydrophobic interactions, however, LF changed surface morphology of zein nanoparticles as observed by scanning electron microscope. X-ray diffraction indicated 7,8-DHF was presented in an amorphous state inside zein/LF nanoparticles. Most importantly, zein/LF-DHF had good redispersibility, and increased the encapsulation efficiency, chemical stability, water solubility and bioaccessibility of 7,8-DHF. Collectively, zein/LF nanoparticles are promising delivery systems for 7,8-DHF in functional foods.


Assuntos
Flavonas/química , Lactoferrina/química , Nanopartículas/química , Zeína/química , Concentração de Íons de Hidrogênio
20.
Carbohydr Polym ; 231: 115725, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31888813

RESUMO

In order to further enhance hydrophilicity of zein and achieve nano-scale pesticide system, phosphorylated zein (P-Zein) was incorporated with graft copolymer CMC-g-PDMDAAC by electrostatic interaction, in which carboxymethyl cellulose (CMC) and diallyldimethylammonium chloride (DMDAAC) as monomers. P-Zein/CMC-g-PDMDAAC was applied to encapsulate model pesticide avermectin (AVM) via electrostatic interaction to achieve AVM@P-Zein/CMC-g-PDMDAAC nano-pesticides. The stability, drug loading, anti-ultraviolet, adhesion, sustained-release and toxicity of nano-pesticides presented considerable behavior confirmed via various characteristics. The results indicated that AVM@P-Zein/CMC-g-PDMDAAC had an average particle size of 360 nm, and possessed favorable dispersion performance and excellent anti-ultraviolet property compared to P-Zein. And comparatively, encapsulation efficiency increased up to 82.11%. In addition, adhesion performance of AVM@P-Zein/CMC-g-PDMDAAC on foliage also improved by about 20% compared to P-Zein. Also, AVM@P-Zein/CMC-g-PDMDAAC can intelligently control pesticide release by adjusting monomer ratio and pH values. More importantly, such nano-pesticide system presented no significant difference on toxicity in comparison with bare AVM.


Assuntos
Carboximetilcelulose Sódica/química , Composição de Medicamentos , Praguicidas/química , Zeína/química , Cápsulas/química , Cápsulas/farmacologia , Concentração de Íons de Hidrogênio , Interações Hidrofóbicas e Hidrofílicas , Ivermectina/análogos & derivados , Ivermectina/química , Ivermectina/farmacologia , Nanopartículas/química , Tamanho da Partícula , Praguicidas/farmacologia , Polietilenos/química , Polietilenos/farmacologia , Compostos de Amônio Quaternário/química , Compostos de Amônio Quaternário/farmacologia
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