Whole-cell bioreporter application for rapid evaluation of hazardous metal bioavailability and toxicity in bioprocess.

Assessing heavy metal bioavailability and toxicity during bioprocess is critical for advancing green biotechnology. The capability of whole-cell bioreporters to measure heavy metal bioavailability has been increasingly recognized. The advantages of this technology being applied to bioprocess monitoring are less studied. Here we investigate the potential of a cadmium- and lead-sensitive bioreporter to be used for heavy metals as a class, which holds great interest for bioprocess applications. We evaluated the bioavailability of eight individual heavy metals with bioreporter zntA, as well as the bioavailability and toxicity of mixed metals. The bioavailability and toxicity of heavy metals in bioprocess samples were also evaluated. We have demonstrated for the first time that the zntA bioreporter can effectively detect the bioavailability of zinc, nickel, and cobalt with limit of detection lower than 0.01, 0.08 and 0.5 mg·L-1, respectively. The detection limits meet the requirements of the WHO, the U.S. Environmental Protection Agency, and the China drinking water quality standards, which makes this approach reasonable for monitoring heavy metal bioavailability in bioprocess. LIVE/DEAD toxicity experiments have been conducted for the detection of mixed metal solution toxicity to zntA bioreporter which shows an EC50 (as EC50, concentration for 50% of maximal effect) value of mixed metal solution is 3.84 mg·L-1. Samples from wastewater treatment plants, sludge treatment plants and kitchen waste fermentation processes were analyzed to extend upon the laboratory results. The results of this study confirm the potential for practical applications of bioreporter technology in bioprocess monitoring. In turn, development for such practical applications is key to achieve the necessary level of commercialization to further make the routine use of bioreporters in bioprocess monitoring feasible.

DNA hypomethylation-associated transcriptional rewiring enables resistance to heavy metal mercury (Hg) stress in rice.

Mercury (Hg) is an important hazardous pollutant that can cause phytotoxicity and harm human health through the food chain. Recently, rice (Oryza sativa L.) has been confirmed as a potential Hg bioaccumulator. Although the genetic and molecular mechanisms involved in heavy metal absorption and translocation in rice have been investigated for several heavy metals, Hg is largely neglected. Here, we analyzed one Hg-resistant line in rice (RHg) derived from a DNA methyltransferase-coding gene, OsMET1-2 heterozygous mutant. Compared with its isogenic wild-type (WT), RHg exhibited a significantly higher survival rate after Hg treatment, ameliorated oxidative damage, and lower Hg uptake and translocation. RNAseq-based comparative transcriptomic analysis identified 34 potential Hg resistance-related genes involved in phytohormone signaling, abiotic stress response, and zinc (Zn) transport. Importantly, the elevated expression of Hg resistance-related genes in RHg was highly correlated with DNA hypomethylation in their putative promoter regions. An ionomic analysis unraveled a negative correlation between Zn and Hg in roots. Moreover, Hg concentration was effectively decreased by exogenous application of Zn in Hg-stressed rice plants. Our findings indicate an epigenetic basis of Hg resistance and reveal an antagonistic relationship between Hg and Zn, providing new hints towards Hg detoxification in plants. ENVIRONMENTAL IMPLICATION: Mercury (Hg) as an important hazardous pollutant adversely impacts the environment and jeopardizes human health, due to its chronicity, transferability, persistence, bioaccumulation and toxicity. In this paper, we identified 34 potential genes that may significantly contribute to Hg resistance in rice. We find the expression of Hg resistance-related genes was highly correlated with DNA hypomethylation in their putative promoter regions. Our results also revealed an antagonistic relationship between Hg and Zinc (Zn), providing new hints towards Hg detoxification in plants. Together, findings of this study extend our current understanding of Hg tolerance in rice and are informative to breed seed non-accumulating rice cultivars.

Titanium modification using bioactive titanate layer with divalent ions and coordinated ciprofloxacin - Assessment of drug distribution using FT-IR imaging.

The paper presents a new method of titanium alloy (Ti6Al4V) modification using bioactive titanate layers containing various divalent ions (Ca2+, Mg2+, Sr2+, Zn2+) and surface-coordinated ciprofloxacin. Due to the coordination of ciprofloxacin (antibiotic) on the surface of the alloy, it has great application potential. In the paper, the influence of a given cation on the effectiveness of drug sorption was determined. The most effective cation was zinc and the least effective was calcium. The distribution of the antibiotic on the alloy surface was determined using FT-IR imaging. The antibiotic was evenly distributed on alloys modified with magnesium, strontium and zinc titanates. In the case of calcium titanate, the analysis could not be performed because the amount of the drug was too small. The release profiles of ciprofloxacin indicate that it can be released for as long as 3 h for strontium and zinc titanates. The biocompatibility of the obtained materials is indicated by the results of the BSA adsorption, and HA growth test. The obtained results confirm that the proposed modification can be used in the modification of titanium implants. The big advantage of this layer is that ciprofloxacin is coordinated on the surface of the material and thus will not be removed during the surgical procedure. The creation of this type of layer may in the future allow for fewer perioperative infections, and thus fewer complications.

Deciphering the complexes of zinc ions and hen egg white lysozyme: Instrumental analysis, molecular docking, and antimicrobial assessment.

In the research presented in this manuscript, an intricate study has been carried out on the interaction of zinc ions with the hen egg white lysozyme (HEWL) protein. Utilizing a spectroscopic technique, the alterations that arise due to the binding of Zn2+ to the HEWL were scrutinized, underscoring the paramount significance of deprotonated carboxyl and thiol groups in the process of binding. The binding phenomena were substantiated using capillary electrophoresis integrated with inductively coupled plasma mass spectrometry (CE-ICP-MS). Further spectrometric assessments (MALDI-TOF MS and FT-ICR-MS) shed light on the direct interaction of zinc ions with the functional groups of the protein. Importantly, high-resolution FT-ICR-MS techniques elucidated the capability of a single protein molecule to bind to multiple zinc ions. The empirically derived spectroscopic data received additional confirmation via a molecular docking study of the Zn2+ binding process, which highlighted a substantial affinity between the predicted 3D model of zinc-lysozyme complexes. Predominantly, the interaction between the bound entities was observed at the cysteine residues. Lastly, the conducted antimicrobial tests revealed that the zinc-lysozyme complexes manifest an inhibitory effect against bacterial (E. coli and S. aureus) and yeast (C. albicans) strains.

Exploring the role of copper and zinc in chronic otitis media: A novel spectrofluorometric method for precise determination and association study.

Chronic otitis media is a common condition characterized by fluid accumulation in the middle ear, leading to a perforated eardrum and persistent middle ear drainage. Despite its impact on global health, the role of heavy metals, particularly copper and zinc, in its development and progression remains understudied. Herein, a spectrofluorometric method was developed for the precise determination of copper and zinc in human plasma samples and investigate their association with chronic otitis media. The method involves the use of the fluorescent probe 6,7-dihydroxy-4-phenylcoumarin to selectively quantify copper through fluorescence quenching and zinc through fluorescence enhancement with a remarked bathochromic shift. The method was validated and exhibited good linearity over a concentration range of 100-3000 ng/mL for copper and 200-5000 ng/mL for zinc. Application of the method to healthy volunteers and patients with chronic otitis media revealed significantly decreased copper and zinc levels in patients with chronic otitis media compared to the healthy individuals. These findings shed light on the involvement of copper and zinc in the pathogenesis of chronic otitis media and open avenues for additional treatment approaches.

Nanomolar electrochemical detection of feed additive ractopamine in meat samples using spinel zinc ferrite decorated 3-dimensional graphene nanosheets to combat food fraud in livestock industries.

Accurate detection of feed additive is significant for food safety monitoring, warding off its illegal use in livestock production, safeguarding public health, and regulatory compliance. Hence, this paper presents a cost-effective and ultrasensitive electrochemical sensor for detecting commonly used animal feed additive, ractopamine to combat food frauds in meat samples. The sensor was created by embedding spinel zinc ferrite nanospheres (ZnFe2O4) on three-dimensional graphene (3DG) nanosheets using sonochemical method. ZnFe2O4 nanospheres were synthesized using solvothermal approach, and 3DG was prepared using hydrothermal method. Various characterization techniques were used for inspecting structural and morphological properties of materials, including XRD, SEM, TEM, elemental mapping, Raman spectroscopy, XPS, and EIS. The hybrid nanocomposite modified electrode showed excellent electrochemical performance, with high sensitivity of 16.97 µA µM-1 cm-2 and detection limit of 1 nM. The practicality of sensor was demonstrated by performing real sample analysis in pork, beef, and sausage which gave adequate recovery.

Prediction of cadmium and zinc phytoextraction by the hyperaccumulator Sedum plumbizincicola using a dynamic geochemical mechanical combination model.

Phytoextraction with hyperaccumulators is an environmentally friendly and cost-effective technique for soil remediation but remediation time is largely dependent on experience due to variations in soil properties which restrict the application of this technique. Here, a novel dynamic multi-surface model (MSM) framework is proposed to predict the efficiency and duration of cadmium (Cd) and zinc (Zn) phytoextraction using the hyperaccumulator Sedum plumbizincicola. First, the application of MSM to S. plumbizincicola was investigated using 95 naturally contaminated soils. Using the 'default' settings and considering the pH and DOC content in the rhizosphere, the dissolved Cd/Zn predicted by MSMs showed strong correlations with metal uptake by shoots (R2 = 0.825/0.802 for Cd/Zn, n = 95) and outperformed chemical extraction methods. Then the MSMs were further integrated with time and Cd and Zn interactions to form dynamic-MSM combined (D-MSM-C) models to evaluate and predict phytoextraction efficiency and duration based on a six-season continuous pot experiment. The D-MSM-C models well predicted metal contents remaining in soils after each season with mean absolute percentage error (MAPE) = 20.4 % (Cd) and 2.46 % (Zn) (n = 66). This model is a powerful tool for assessing and predicting phytoremediation efficiency and duration and is applicable across diverse soil properties and multiple metal-contamination scenarios.

Does a decade of soil organic fertilization promote copper and zinc phytoavailability? Evidence from a laboratory biotest with field-collected soil samples.

Despite chronic contamination, long-term organic fertilization tends to decrease copper (Cu) and zinc (Zn) availability in agricultural soils. Root activities of crop plants can also induce substantial changes in rhizosphere chemistry and consequently in the Cu and Zn availability in the rhizosphere. The balance between these two drivers and the overall effect of organic fertilization on Cu and Zn bioavailability to plants (i.e., phytoavailability) remains a matter of debate. We assessed the effect of a decade of agronomically realistic organic fertilization on Cu and Zn availability in the rhizosphere and their phytoavailability. Using a laboratory biotest, Festuca arundinacea was exposed to 34 soil samples collected from three agricultural field trials that had received no, mineral, or organic fertilization for a decade. Dissolved organic matter (DOM) properties (i.e., concentration, aromaticity, and binding properties toward Cu), pH, and Cu and Zn availability (i.e., total dissolved concentration and free ionic activity) were determined in the rhizosphere solutions. Cu and Zn phytoavailability was measured as the plant uptake flux. Contrary to bulk soils, organic fertilization induced very few changes in the chemistry and Cu and Zn availability in the rhizosphere solutions compared to no and mineral fertilization. Consistently, Cu and Zn phytoavailability did not increase with organic fertilization, but it was mostly driven by soil properties rather than by fertilization. Despite increasing soil Cu and Zn contamination, a decade of soil organic fertilization did not increase Cu and Zn phytoavailability, presumably due to the root-mediated levelling of Cu and Zn availability in the rhizosphere.

Fe and Zn co-doped carbon nanoparticles as peroxymonosulfate activator for efficient 2,4-dichorophenol degradation.

Iron-mediated activation of peroxymonosulfate (PMS) has been of great interest for the effective removal of contaminants, but it still suffered from ineffective metal redox cycle rate, which resulted in unsatisfactory catalytic efficiency. Constructing bimetallic carbonaceous materials was effective way to improve the catalytic performance of iron-based heterogeneous system. In this study, magnetic bimetallic porous carbon composite (FZCx) was synthesized via Fe/Zn bi-MOFs pyrolysis for 2,4-dichlorophenol (2,4-DCP) degradation by peroxymonosulfate. Influences of different systems exhibited that 100% of 2,4-DCP was rapidly degraded at the conditions of catalyst dosage = 0.1 g L-1, PMS = 0.5 mM and initial pH = 9.0 within 30 min. The as-prepared FZC600 displayed excellent reusability and stability. Quenching experiments and EPR analysis manifested that SO4·- and 1O2 were primarily responsible for the rapid degradation of 2,4-DCP. Moreover, XPS, EPR and EIS was used to elaborate the bimetallic synergy effect, proving that the introduction of zinc can effectively promote periodic cycle of Fe2+/Fe3+ and improve catalysts durability and reusability. These findings highlighted the preparation of bimetallic based carbonaceous material with excellent PMS activation ability to remove refractory organics from wastewater and provided a depth insight into the promotion of bimetal synergy between zinc and iron on PMS activation process.

Tafluprost promotes axon regeneration after optic nerve crush via Zn2+-mTOR pathway.

PURPOSE: To investigate whether Tafluprost could promote optic nerve regeneration in mice after optic nerve crush (ONC) and determine the underlying molecular mechanism. METHODS: Tafluprost was injected into the vitreous body immediately after ONC. The level of Zn2+ in the inner plexiform layer (IPL) of the retina was stained using autometallography (AMG). The number of survival retinal ganglion cells (RGCs) was determined via dual staining with RGC markers Tuj1 and RBPMS. Individual axons that regenerated to 0.25, 0.5, 0.75 and 1 mm were manually counted in the whole-mount optic nerve labeled by cholera toxin B fragment (CTB). Immunofluorescence and Western blot were performed to detect protein expression levels. Pattern electroretinogram was used to evaluate RGCs function. RESULTS: Tafluprost promoted RGC survival in a dose-dependent manner with an optimal concentration of 1 µM. Tafluprost significantly decreased ZnT-3 expression and Zn2+ accumulation in the IPL of retina. Tafluprost stimulated intense axonal regeneration and maintained RGCs function compared to control. Mechanistically, Tafluprost and Zn2+ elimination treatment (TPEN or ZnT-3 deletion) can activate the mTOR pathway with an improved percentage of pS6+ RGCs in the retina. However, rapamycin, a specific inhibitor of the mTOR1, inhibited the activation of the mTOR pathway and abolished the regenerative effect mediated by Tafluprost. Tafluprost also inhibited the upregulation of p62, LC3 and Beclin-1, attenuated the overactivation of microglia/macrophages and downregulated the expression of TNFα and IL-1ß. CONCLUSIONS: Our results suggest that Tafluprost promoted axon regeneration via regulation of the Zn2+-mTOR pathway, and provide novel research directions for glaucomatous optic nerve injury mechanisms.

Mechanistic insights into synergistic facilitation of copper/zinc ions and dewatered swine manure-derived biochar on anaerobic digestion of swine wastewater.

Biochar-assisted anaerobic digestion (AD) has been proposed an advanced system for swine wastewater (SW) management. However, the effects of metallic nutrients in SW, such as copper/zinc ions (Cu2+/Zn2+), on the biochar-assisted AD of SW are not well understood. This study investigated the influences of individual Cu2+/Zn2+ or dewatered swine manure-derived biochar, as well as their combined additions, on the AD of SW. The results showed that exposure to 50 mg/L Cu2+/Zn2+ temporary inhibited methane production, but the addition of 20 g/L biochar alleviated this inhibition by shortening the methanogenic lag time and increasing methane yield. Following a period of acclimation, both Cu2+/Zn2+ and biochar promoted methane production, although metagenomic analysis revealed distinct mechanisms underlying their promotion. Cu2+/Zn2+ enhanced ATP processing, including electron exchange between NADH/NAD+ and succinate/fumarate transformation, by 26.0-35.8%. Additionally, the gene encoding Coenzyme M methylation was upregulated by 36.2% along with enrichments of Methanocullus and Methanosarcina, contributing to accelerated hydrolysis and methanogenesis rates by 54.7% and 44.8%, respectively. On the other hand, biochar mainly stimulated bacterial F-type ATPase activities by 28.4%, likely facilitating direct interspecies electron transfer between Geobacter and Methanosarcina for syntrophic methanogenesis. The combined addition of Cu2+/Zn2+ and biochar resulted in "win-win" benefits, significantly increasing the maximum methane production rate from 40.3 mL CH4/d to 53.7 mL CH4/d. Moreover, the introduction of biochar into AD of SW facilitated the transformation of more Cu2+/Zn2+ from a reducible Fe-Mn oxides form to a residual form, which potentially reduced the metallic toxicity of the digestate for soil amendment. The findings of this study provide novel insights into understanding the synergistic impacts of heavy metals and biochar in regulating SW during AD, as well as the management of associated digestate.

Geochemical properties, heavy metals and soil microbial community during revegetation process in a production Pb-Zn tailings.

Lead-zinc (Pb-Zn) tailings pose a significant environmental threat from heavy metals (HMs) contamination. Revegetation is considered as a green path for HM remediation. However, the interplay between HM transport processes and soil microbial community in Pb-Zn tailings (especially those in production) remain unclear. This study investigated the spatial distribution of HMs as well as the crucial roles of the soil microbial community (i.e., structure, richness, and diversity) during a three-year revegetation of production Pb-Zn tailings in northern Guangdong province, China. Prolonged tailings stockpiling exacerbated Pb contamination, elevating concentrations (from 10.11 to 11.53 g/kg) in long-term weathering. However, revegetation effectively alleviated Pb, reducing its concentrations of 9.81 g/kg. Through 16 S rRNA gene amplicon sequencing, the dominant genera shifted from Weissella (44%) to Thiobacillus (17%) and then to Pseudomonas (comprising 44% of the sequences) during the revegetation process. The structural equation model suggested that Pseudomonas, with its potential to transform bioavailable Pb into a more stable form, emerged as a potential Pb remediator. This study provides essential evidence of HMs contamination and microbial community dynamics during Pb-Zn tailings revegetation, contributing to the development of sustainable microbial technologies for tailings management.

Effects of seasonal temperature regimes: Does Cyprinus carpio act as a health hazard during the construction of Suki Kinari hydropower project on Kunhar River in Pakistan?

The main purpose of the current study was to assess the levels of trace elements (iron, lead, zinc, copper, and manganese) in both water and fish muscles of common carp (Cyprinus carpio) in the Kunhar River during the development of the Suki Kinari hydropower project (SKHPP). Additionally, the aim was to shed light on the potential health hazards associated with the consumption of fish by residents. Surface water and fish (muscle and liver) from ten specific sampling locations in the Mansehra district (affected by an SKHPP) along the river were examined to determine the levels of trace elements. The findings divulged that the water at all locations exhibited concentration levels of iron (Fe), lead (Pb), and manganese (Mn) that surpassed the benchmarks established by the World Health Organization in 2011. Conversely, the concentration levels of copper (Cu) and zinc (Zn) fell beneath the stipulated standards. Moreover, the concentrations of Mn, Zn, and Pb were found to be excessively high. The findings presented in the present study offer a comprehensive comprehension of the spatial and distribution characteristics of trace elements in both water and fish species along the Kunhar River, taking into consideration the impact of the SKHPP. Additionally, our data emphasize the potential health hazards that may arise from the prolonged consumption of fish by the local population.

METTL3 and METTL14 regulate IL-6 expression via RNA m6A modification of zinc transporter SLC39A9 and DNA methylation of IL-6 in periodontal ligament cells.

The inflammatory response is a key process in periodontitis. The N6-methyladenosine (m6A) modification has been proven to be involved in various physiological and pathological processes. This study aims to investigate the role and downstream mechanism of N6-adenosine-enzyme subunits methyltransferase (METTL) 3 and 14 in the inflammatory response of periodontal ligament cells (PDLCs). The total m6A content and the expression of METTL3 and METTL14 were upregulated in lipopolysaccharide (LPS)-stimulated PDLCs. Knockdown of METTL3 or METTL14 suppressed the LPS-induced interleukin (IL)-6 expression, as shown by quantitative polymerase chain reaction (qPCR) and enzyme linked immunosorbent assay (ELISA). Mechanistically, conjoint analysis of m6A sequencing of METTL3-knockdown and METTL14-knockdown PDLCs revealed that the expression of solute carrier family 39 member 9 (SLC39A9) was mediated in a m6A-dependent manner. The suppression of LPS-induced IL-6 by METTL3 or METTL14 knockdown was partially counteracted by SLC39A9 knockdown, which induced downregulation of intracellular zinc via immunofluorescence staining. Amplicon bisulfite sequencing (AmpBS) demonstrated that METTL3/14 knockdown increased the methylation at one position of the IL-6 promoter, while SLC39A9 knockdown decreased it, which was basically consistent with the intracellular zinc concentration and negatively associated with IL-6 expression. Moreover, METTL3 or METTL14 knockdown attenuated the LPS-induced phosphorylation of p38 and JNK mitogen-activated protein kinase (MAPK), which was partially counteracted by SLC39A9 knockdown. These results revealed the "LPS-METTL3/14-SLC39A9-zinc-IL-6" axis and involvement of p38 and JNK MAPK signaling pathway in the inflammatory responses of PDLCs.

Dietary collagen peptide-chelated trace elements supplementation for breeder hens improves the intestinal health of chick offspring.

BACKGROUND: Dietary supplementation with trace elements zinc (Zn), iron (Fe) and manganese (Mn) could promote intestinal development and improve intestinal health. There are, however, few studies examining the possibility that maternal original Zn, Fe and Mn could regulate intestinal development and barrier function in the offspring. This study aimed to investigate how the intestinal growth and barrier function of breeder offspring were affected by collagen peptide-chelated trace elements (PTE; Zn, Fe, Mn). RESULTS: PTE supplementation in the diet of breeder hens increased the concentrations of Zn, Fe and Mn in egg yolk. Maternal PTE supplementation improved morphological parameters of the intestine (villi height, crypt depth and villi height/crypt depth) and upregulated the mRNA expression level of leucine-rich repeat-containing G protein-coupled receptor 5 (Lgr5) in the ileum of chick embryos. Furthermore, maternal PTE effect improved villi height/crypt depth of offspring at 1 and 14 days of age, and upregulated Lgr5, Claudin-3 and E-cadherin mRNA expression in the broiler ileum. Additionally, PTE treatment could enhance the intestinal microbial diversity of offspring. Maternal PTE supplementation increased the relative abundance of Clostridiales at the genus level and decreased the relative abundance of Enterococcus in newborn offspring. Moreover, maternal PTE supplementation ameliorated the elevated nuclear factor kappa B, toll-like receptor 4 and interleukin 1ß mRNA expression in the ileum of offspring caused by LPS challenge. CONCLUSION: Maternal PTE supplementation could promote intestinal development and enhance the intestinal barrier function of chicken offspring. © 2023 Society of Chemical Industry.

Foliar co-application of zinc oxide and copper oxide nanoparticles promotes phytochemicals and essential oil production in dragonhead (Dracocephalum moldavica).

Individual nanoparticle application has been documented to promote plant production; however, whether co-application of two nanoparticles (NPs) is more sustainable and significantly promotes plant production is unclear. Herein, foliar co-applications of two NPs or their conventional fertilizer forms on the growth, micronutrient (copper and zinc) enrichment, primary productivity, and essential oil (EO) production in a medicinal annual, dragonhead (Dracocephalum moldavica L.), were investigated. Treatments included 1:1 ratio of zinc oxide nanoparticles (ZnONPs):copper oxide nanoparticles (CuONPs) (40-400 mg/L), and compared with individual NPs, individual zinc suspension (ZnS) and chelated copper (chelated-Cu), and their combination, at equivalent concentrations. Results showed that the highest bioenrichment of Zn and Cu was observed with 80-160 mg/L ZnS+chelated-Cu, 400 mg/L ZnONPs+CuONPs, or ionic combination treatments. A dose-dependent increase in hydrogen peroxide and malondialdehyde was observed with co-treatment of NPs or ions, and oxidative stress responses were higher with NPs or ions co-treatment than individual treatment. With 160 mg/L ZnONPs+CuONPs treatment, total chlorophyll, aboveground biomass, and essential oil production increased significantly compared to control, 160 mg/L CuONPs, and 160 mg/L ZnONPs (227, 157 and 823 %; 58, 79, and 51 %; and 46, 80, and 3 %, respectively). Flavonoid and anthocyanin content also increased significantly (58 and 50 %, respectively) with ZnONPs+CuONPs compared to ZnS+chelated-Cu and were higher than ZnONPs or CuONPs alone by 10 and 25 %, and 37 and 36 %, respectively. More importantly, EO production and quality improved with 160 mg/L ZnONPs+CuONPs treatment compared to control. Taken together, our findings showed that foliar co-treatment of 160 mg/L ZnONPs+CuONPs could significantly improve primary productivity, aboveground biomass, and EO quality and yield in dragonhead grown in semi-arid field conditions; and thus, 160 mg/L ZnONPs+CuONPs is recommended as an optimal foliar co-treatment strategy for promoting sustainable plant production in semi-arid regions where soil nutrients and water are limiting factors inhibiting crop yield.

Roles of zinc in cancers: From altered metabolism to therapeutic applications.

Zinc (Zn) is a crucial trace element involved in various cellular processes, including oxidative stress, apoptosis and immune response, contributing to cellular homeostasis. Dysregulation of Zn homeostasis occurs in certain cancers. This review discusses the role of Zn in cancer and its associated components, such as Zn-related proteins, their potential as biomarkers and the use of Zn-based strategies for tumor treatment. ZIP and ZnT proteins regulate Zn metabolism under normal conditions, but their expression is aberrant in cancer. These Zn proteins can serve as prognostic or diagnostic biomarkers, aiding in early cancer detection and disease monitoring. Moreover, targeting Zn and its pathways offers potential therapeutic approaches for cancer treatment. Modulating Zn biodistribution within cells using metal-binding agents allows for the control of downstream signaling pathways. Direct utilization of zinc as a therapeutic agent, including Zn supplementation or Zn oxide nanoparticle administration, holds promise for improving the prognosis of cancer patients.

Novel α-amino acid-like structure decorated biochar for heavy metal remediation in acid soil.

Neither chemical nor physical adsorption play well in heavy metals remediation in acid soil due to the competing behavior of abundant protons, where stable chelators that can be reused are of significant demand. Herein, biochar with abundant nitro and carboxyl groups is prepared, which can be assembled into self-supporting electrode. Under the catalyzation of electricity, the surface decorated -NO2 on the biochar can be in situ transformed into -NH2. Combined with the carboxyl group that attached on the same carbon atom, a special α-amino acid-like structure modified biochar (α-AC@BC) can be successfully constructed. Due to the strong affinity between the α-amino acid-like ligand and heavy metals, this α-AC@BC exhibits high removal efficiencies of 83.41%, 80.94%, 92.54% and 77.05% for available copper, cadmium, lead and zinc respectively, even in a strong acid soil with low pH of 4. After four adsorption-desorption cycles, the α-AC@BC could still eliminate 83.88% of copper. The high adsorption energy among -NH2, -COOH and heavy metals (-2.99 eV for copper, -1.90 eV for lead, -1.30 eV for zinc and -0.91 eV for cadmium) could form steady coordination structure to guarantee a highly practical application potential of α-AC@BC in strong acid soil. This study provides a novel concept for the decontamination of multiple heavy metal polluted acid soil.

Preparation of fluorescent sensor based on Zn metal-organic framework for detection and determination of raloxifene as an anticancer drug.

Breast cancer is the second leading cause of death for women worldwide. Raloxifene (RLX) is a somewhat effective drug in lowering cholesterol, preventing and treating invasive breast cancer in postmenopausal women with osteoporosis, and does not interfere with breast tissue. Nevertheless, considering the possibility of risk in biological function due to excessive use of anticancer drugs and the adverse effects of drugs in wastewater on plants, animals, and aquatic, it is useful to determine the concentration of RLX in water and human body fluids. Here, a fluorescent metal-organic framework (MOF) nanoparticle based on trinuclear zinc clusters called Zn-MOF was presented, which is a high-performance and fast-response fluorescent chemosensor that can be used to detect RLX based on the fluorescence quenching medium in water. FTIR, XRD, SEM, and EDS analyses were used to identify the functional group and determine the structure and morphology of Zn-MOF. pH range 3-10. The prepared nanoparticles showed symmetric emission with excitation at a wavelength of 310.0 nm. The performance of the proposed fluorescent nanosensor was proportional to the quenching of the fluorescent signal with increasing RLX concentration at 404.0 nm; the quenching fluorescence response was linear in RLX concentration from 0.7 to 350 ng/mL with a significant detection limit equal to 0.485 nM.

A smartphone-assisted electrochemiluminescent detection of miRNA-21 in situ using Ru(bpy)32+@MOF.

We have proposed a signal dual-amplification electrochemiluminescence (ECL) strategy based on tris(2,2'-bipyridyl)ruthenium(II) (Ru(bpy)32+) as chromophores confined with three-dimensional (3D) zinc oxalate metal-organic frameworks (Ru(bpy)32+@MOFs) for the detection of miRNA-21. The three-dimensional chromophore connectivity in zinc oxalate MOFs provided a network among Ru(bpy)32+ units, shielding the chromophores from solvent molecules and resulting in high Ru(II) complex emission efficiency. Additionally, we discovered that magnetic beads (MBs) used as carrier for enriched signals contribute to enhanced ECL intensity of the chromophore. To evaluate its clinical application, we applied this method to determine the concentration of miRNA-21 solutions ranging from 1.56 to 100 nM, obtaining a calibration curve of ECL intensity versus logarithm of concentration (logC) of miRNA-21 with a high correlation coefficient. This work demonstrates the construction of a signal amplification strategy ECL biosensor for miRNA using Ru(bpy)32+@MOF systems and its application in ECL detection for analyte methodology.