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1.
Medicine (Baltimore) ; 99(1): e18632, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31895823

RESUMO

Health related quality of life (HRQOL) in chronic liver disease (CLD) patients has been attracting much attention these days because it is closely associated with clinical outcomes in CLD patients. HRQOL has become established as an important concept and target for research and practice in the fields of medicine. A critique of HRQOL research is the lack of conceptual clarity and a common definition of HRQOL. Using a clear definition of HRQOL may increase the conceptual understanding. In this study, we aimed to elucidate the association between serum zinc (Zn) level and HRQOL as assessed by the Beck Depression Inventory-2nd edition (BDI-II), Pittsburgh Sleep Quality Index Japanese version (PSQI-J) and the 36-Item Short Form Health Survey (SF-36) in CLD patients (n = 322, median age = 65 years, 121 liver cirrhosis (LC) patients (37.6%)). The median serum Zn level for all cases was 73.2 µg/dl. The median BDI-II score and PSQI-J score were 6 and 5, respectively. Patients with higher BDI-II score tended to have lower serum Zn level compared with those with lower BDI-II score. Similar tendencies were observed in patients with higher PSQI-J score. In the SF-36, physical functioning, role physical and physical component summary score significantly correlated with serum Zn level regardless of age, liver disease etiology and the LC status. While mental health and mental component summary score did not significantly correlate with serum Zn level regardless of age, liver disease etiology and the LC status. In conclusion, serum Zn level can be a useful marker for decreased HRQOL in patients with CLDs, especially for physical components.


Assuntos
Hepatopatias/sangue , Qualidade de Vida , Zinco/sangue , Idoso , Feminino , Humanos , Hepatopatias/psicologia , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos
2.
An Bras Dermatol ; 94(6): 713-716, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31789267

RESUMO

Hyperzincemia and hypercalprotectinemia is a rare inflammatory disease caused by a mutation in the PSTPIP1 gene, with a dysregulation of calprotectin metabolism. Calprotectin is a zinc-binding protein with antimicrobial properties and pro-inflammatory action. The authors report the case of a 20 year-old girl with cutaneous ulcers comparable with pyoderma gangrenosum, growth failure and chronic anemia, who was given the diagnosis of hyperzincemia and hypercalprotectinemia. Measurement of serum zinc and calprotectin concentrations are indicated in these cases.


Assuntos
Erros Inatos do Metabolismo dos Metais/patologia , Pioderma Gangrenoso/patologia , Ciclosporina/uso terapêutico , Fármacos Dermatológicos/uso terapêutico , Feminino , Glucocorticoides/uso terapêutico , Humanos , Complexo Antígeno L1 Leucocitário/sangue , Erros Inatos do Metabolismo dos Metais/tratamento farmacológico , Prednisolona/uso terapêutico , Resultado do Tratamento , Adulto Jovem , Zinco/sangue
3.
Environ Pollut ; 255(Pt 2): 113325, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31614327

RESUMO

Associations between single metal and fasting blood glucose (FBG) levels have been reported in previous studies. However, the association between multi-metals exposure and FBG level are little known. To assess the joints of arsenic (As), nickel (Ni), cadmium (Cd), selenium (Se), and zinc (Zn) co-exposure on FBG levels, Bayesian kernel machine regression (BKMR) statistical method was used to estimate the potential joint associations between As, Ni, Cd, Se, and Zn co-exposure and FBG levels among 1478 community-based Chinese adults from two counties, Shimen (n = 696) and Huayuan (n = 782), with different exposure profiles in Hunan province of China. The metals levels were measured in spot urine (As, Ni, and Cd) and plasma (Se and Zn) using inductively coupled plasma-mass spectrometry, respectively. The exposure levels of all the five metals were significantly higher in Shimen area (median: As = 57.76 µg/L, Cd = 2.75 µg/L, Ni = 2.73 µg/L, Se = 112.67 µg/L, Zn = 905.68 µg/L) than those in Huayuan area (As = 41.14 µg/L, Cd = 2.22 µg/L, Ni = 1.88 µg/L, Se = 65.59 µg/L, Zn = 819.18 µg/L). The BKMR analyses showed a significantly positive over-all effect of the five metals on FBG levels when metals concentrations were all above the 50th percentile while a statistically negative over-all effect when metals concentrations were all under the 50th percentile in Shimen area. However, a totally opposite over-all effect of the mixture of the five metals on FBG levels was found in Huayuan area. BKMR also revealed a non-linear exposure-effect of Zn on FBG levels in Huayuan area. In addition, interaction effects of As and Se on FBG level were observed. The relationship between single or combined metals exposure and FBG was different against different exposure profiles. Potential interaction effects of As and Se on FBG levels may exist.


Assuntos
Arsênico/urina , Glicemia/efeitos dos fármacos , Cádmio/urina , Níquel/urina , Selênio/sangue , Zinco/sangue , Adulto , Idoso , Teorema de Bayes , China , Exposição Ambiental/efeitos adversos , Feminino , Humanos , Masculino , Metais Pesados/toxicidade , Pessoa de Meia-Idade , Adulto Jovem
5.
Int J Mol Sci ; 20(16)2019 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-31426452

RESUMO

Zinc is an essential trace element, making it crucial to have a reliable biomarker for evaluating an individual's zinc status. The total serum zinc concentration, which is presently the most commonly used biomarker, is not ideal for this purpose, but a superior alternative is still missing. The free zinc concentration, which describes the fraction of zinc that is only loosely bound and easily exchangeable, has been proposed for this purpose, as it reflects the highly bioavailable part of serum zinc. This report presents a fluorescence-based method for determining the free zinc concentration in human serum samples, using the fluorescent probe Zinpyr-1. The assay has been applied on 154 commercially obtained human serum samples. Measured free zinc concentrations ranged from 0.09 to 0.42 nM with a mean of 0.22 ± 0.05 nM. It did not correlate with age or the total serum concentrations of zinc, manganese, iron or selenium. A negative correlation between the concentration of free zinc and total copper has been seen for sera from females. In addition, the free zinc concentration in sera from females (0.21 ± 0.05 nM) was significantly lower than in males (0.23 ± 0.06 nM). The assay uses a sample volume of less than 10 µL, is rapid and cost-effective and allows us to address questions regarding factors influencing the free serum zinc concentration, its connection with the body's zinc status, and its suitability as a future biomarker for an individual's zinc status.


Assuntos
Fluoresceínas/química , Corantes Fluorescentes/química , Zinco/sangue , Biomarcadores/análise , Biomarcadores/sangue , Feminino , Humanos , Limite de Detecção , Masculino , Espectrometria de Fluorescência/métodos , Zinco/análise
6.
Acta Neurobiol Exp (Wars) ; 79(2): 184-192, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31342954

RESUMO

Depression is a chronic illness of unknown etiology. Trace elements, such as copper and zinc, and defense antioxidants, such as catalase, are important factors that determine the clinical course of brain diseases. Furthermore, altered glucose metabolism in hippocampus and prefrontal cortex has been associated with depression. Identifying factors that can precipitate depressive-like behavior is of particular importance as it can direct clinicians towards the etiology of the disease. In this study, 16 male Sprague-Dawley rats were randomly divided into two groups: socialized and socially isolated. After one week of acclimatization, animals were housed in isolation for 14 days. Rats in the social group were socialized together for 14 days. On day 15, the forced swim test was performed and blood sugar was analyzed. The brain was removed immediately for biochemical analysis. Socially isolated rats showed more pronounced depressive-like behavior in the forced swim test than socialized rats. Moreover, socially isolated rats demonstrated significantly lower copper and zinc concentrations, as well as a marked reduction in catalase activity, in both prefrontal cortex and hippocampus compared to socialized rats. Additionally, blood sugar levels were higher in socially isolated animals. Isolation causes reduction in copper and zinc levels and catalase activity, which may precipitate depressive-like behavior in these animals.


Assuntos
Catalase/metabolismo , Cobre/sangue , Hipocampo/metabolismo , Estresse Psicológico/fisiopatologia , Zinco/sangue , Animais , Antioxidantes/metabolismo , Depressão/metabolismo , Transtorno Depressivo/fisiopatologia , Hipocampo/fisiopatologia , Masculino , Córtex Pré-Frontal/metabolismo , Córtex Pré-Frontal/fisiopatologia , Ratos Sprague-Dawley , Isolamento Social
7.
Nutrients ; 11(7)2019 Jul 16.
Artigo em Inglês | MEDLINE | ID: mdl-31315178

RESUMO

Trace elements such as zinc, copper, and selenium are essential for reproductive health, but there is limited work examining how circulating trace elements may associate with fertility in humans. The aim of this study was to determine the association between maternal plasma concentrations of zinc, copper, and selenium, and time to pregnancy and subfertility. Australian women (n = 1060) who participated in the multi-centre prospective Screening for Pregnancy Endpoints study were included. Maternal plasma concentrations of copper, zinc and selenium were assessed at 15 ± 1 weeks' gestation. Estimates of retrospectively reported time to pregnancy were documented as number of months to conceive; subfertility was defined as taking more than 12 months to conceive. A range of maternal and paternal adjustments were included. Women who had lower zinc (time ratio, 1.20 (0.99-1.44)) or who had lower selenium concentrations (1.19 (1.01-1.40)) had a longer time to pregnancy, equivalent to a median difference in time to pregnancy of around 0.6 months. Women with low selenium concentrations were also at a 1.46 (1.06-2.03) greater relative risk for subfertility compared to women with higher selenium concentrations. There were no associations between copper and time to pregnancy or subfertility. Lower selenium and zinc trace element concentrations, which likely reflect lower dietary intakes, associate with a longer time to pregnancy. Further research supporting our work is required, which may inform recommendations to increase maternal trace element intake in women planning a pregnancy.


Assuntos
Cobre/sangue , Infertilidade Feminina/sangue , Estado Nutricional , Fenômenos Fisiológicos da Nutrição Pré-Natal , Selênio/sangue , Zinco/sangue , Adolescente , Adulto , Feminino , Humanos , Gravidez , Fatores de Risco , Adulto Jovem
8.
Medicina (Kaunas) ; 55(7)2019 Jul 03.
Artigo em Inglês | MEDLINE | ID: mdl-31277328

RESUMO

Background and objective: Often micronutrient deficiencies cannot be detected when patient is already following a long-term gluten-free diet with good compliance (LTGFDWGC). The aim of this narrative review is to evaluate the most recent literature that considers blood micronutrient deficiencies in LTGFDWGC subjects, in order to prepare dietary supplementation advice (DSA). Materials and methods: A research strategy was planned on PubMed by defining the following keywords: celiac disease, vitamin B12, iron, folic acid, and vitamin D. Results: This review included 73 studies. The few studies on micronutrient circulating levels in long-term gluten-free diet (LTGFD) patients over 2 years with good compliance demonstrated that deficiency was detected in up to: 30% of subjects for vitamin B12 (DSA: 1000 mcg/day until level is normal, then 500 mcg), 40% for iron (325 mg/day), 20% for folic acid (1 mg/day for 3 months, followed by 400-800 mcg/day), 25% for vitamin D (1000 UI/day or more-based serum level or 50,000 UI/week if level is <20 ng/mL), 40% for zinc (25-40 mg/day), 3.6% of children for calcium (1000-1500 mg/day), 20% for magnesium (200-300 mg/day); no data is available in adults for magnesium. Conclusions: If integration with diet is not enough, starting with supplements may be the correct way, after evaluating the initial blood level to determine the right dosage of supplementation.


Assuntos
Doença Celíaca/dietoterapia , Dieta Livre de Glúten/efeitos adversos , Micronutrientes/análise , Adolescente , Adulto , Cálcio/análise , Cálcio/sangue , Doença Celíaca/sangue , Doença Celíaca/tratamento farmacológico , Criança , Dieta Livre de Glúten/métodos , Suplementos Nutricionais/normas , Feminino , Ácido Fólico/análise , Ácido Fólico/sangue , Humanos , Ferro/análise , Ferro/sangue , Masculino , Micronutrientes/sangue , Cooperação do Paciente/psicologia , Vitamina B 12/análise , Vitamina B 12/sangue , Vitamina D/análise , Vitamina D/sangue , Zinco/análise , Zinco/sangue
9.
Talanta ; 204: 663-669, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31357350

RESUMO

A high-throughput, sensitive and rapid method was developed for the determination of Zn, Cu and Fe in small volumes (30 µL) of human serum using inductively coupled plasma mass spectrometry (ICP-MS). The sample preparation procedure employed simple 100-fold dilution of the serum samples with 1.0% butanol, 0.5% v/v ammonia, 0.02% v/v Triton X-100 and 0.01% v/v HNO3. The reliability of the method was evaluated using serum UTAK certified reference material, and the results matched well with the certified values. The method was applied to determine Zn, Cu and Fe in 81 human serum samples from participants in Alzheimer disease (AD) and age-related macular degeneration (AMD) studies. No significant differences were found in Zn and Cu levels between age matched controls, AD and AMD patients. Whilst iron levels appeared marginally higher in the AMD group, compared with the AD group, iron showed larger overall variability than the other two elements.


Assuntos
Cobre/sangue , Ferro/sangue , Espectrometria de Massas/métodos , Zinco/sangue , Doença de Alzheimer/sangue , Amônia/química , Humanos , Degeneração Macular/sangue , Ácido Nítrico/química , Octoxinol/química
11.
J Vet Diagn Invest ; 31(4): 537-545, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31170897

RESUMO

Zinc oxide (ZnO) is commonly fed to pigs at pharmacologic concentrations (2,000-3,000 ppm) for the first 3 wk post-weaning to increase growth and reduce enteric bacterial disease. The safety of this high-dose treatment is assumed based upon lower bioavailability of ZnO compared to other common forms of Zn in feed; however, limited data are available regarding the specific serum and tissue concentrations of Zn expected in animals experiencing overload following feeding of excessive ZnO. Fifty-five 3-wk-old pigs were divided into 5 groups receiving various concentrations of ZnO (0-6,000 ppm) for 3 wk. Pigs receiving 6,000 ppm ZnO had higher mean pancreatic Zn concentrations (p < 0.001) compared to other treatments, and higher pancreatic Zn concentrations were associated with pancreatic acinar cell apoptosis (p < 0.0001). Hepatic Zn concentrations were highest for pigs receiving 6,000 ppm ZnO (mean ± SEM; 729 ± 264 ppm) and significantly higher than all other groups (p < 0.0001), with controls having concentrations <60 ppm. Similarly, serum Zn was highest in pigs receiving 6,000 ppm ZnO (4.81 ± 2.31 ppm) and significantly higher than all groups (controls, <1 ppm). Additionally, as pigs became overloaded with Zn, there were significant reductions in serum Cu and both serum and hepatic Se. Hepatic and serum Zn concentrations >500 ppm and >2 ppm, respectively, are indicative of Zn overload, and dietary trace mineral analysis is warranted if expected inclusion rates are ≤3,000 ppm ZnO.


Assuntos
Doenças dos Suínos/induzido quimicamente , Óxido de Zinco/administração & dosagem , Zinco/sangue , Ração Animal/análise , Animais , Dieta/veterinária , Suplementos Nutricionais , Fígado/efeitos dos fármacos , Suínos , Óxido de Zinco/química
12.
Folia Med (Plovdiv) ; 61(1): 113-119, 2019 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31237858

RESUMO

BACKGROUND: Combined exposure to heavy metals could be expected among residents of two villages in Stara Zagora Municipality due to significant pollution sources in the region, confirmed by increased heavy metal concentrations in plant samples identified by previous studies. The risk is increased for retired villagers who consume food produced in their own farms. AIMS: To determine blood levels of lead, cadmium and zinc and the correlation between them in high-risk elderly rural inhabitants in comparison with a control group. MATERIALS AND METHODS: Lead, cadmium and zinc concentrations in whole venous blood were measured by atomic absorption spectrometry in exposed and in control groups adjusted by sex, age, smoking habit, lifestyle and duration of residence in the settlements. RESULTS: Blood values of lead and cadmium in the exposed group were significantly higher than these in the control group (p<0.001). A statistically significant positive correlation between lead and cadmium blood levels was estimated for the exposed group (ρ=0.39, p=0.023). Blood zinc levels correlated negatively with both lead (ρ=-0.41, p=0.015) and cadmium blood levels (ρ=-0.44, p=0.009). No correlations between the studied metals were found in the control group. CONCLUSION: The observed results could be explained by a long-term combined exposure to lead and cadmium in the studied elderly residents. The negative correlation of zinc blood levels versus lead and cadmium could be result of competition. Complete protein intake and supplementation with zinc, calcium and iron after control measurements are advisable for elderly rural inhabitants to reduce the health risk from heavy metal exposure.


Assuntos
Cádmio/sangue , Chumbo/sangue , Zinco/sangue , Idoso , Suplementos Nutricionais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , População Rural
13.
Clin Lab ; 65(6)2019 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-31232016

RESUMO

BACKGROUND: Zinc and copper are among the most important trace elements. Deficiencies of these trace elements cause a wide variety of disorders. The present study aims to report the definitive assessment of biological variation (BV) parameters for these elements as within-subject BV (CVI), between subject BV (CVG), index of individuality (II), and reference change value (RCV) in a Turkish cohort study group. METHODS: Ten blood specimens were collected weekly from 20 healthy volunteers (13 women, 7 men) for 10 weeks. Collected sera were stored at -80°C until the time of analysis. Serum zinc and copper levels were analyzed with atomic absorption spectrometry and ANOVA test was used to calculate the variations. RESULTS: The CVI and CVG for zinc were 6.26% and 23.27%, respectively. Analytical variation (CVA) was calculated as 4.24%. II and RCV for zinc were calculated as 0.26 and 21.51%, respectively. The CVI and CVG for copper were 6.05% and 19.64%, respectively. CVA was calculated as 4.24%. II and RCV for copper were calculated as 0.31 and 20.47%, respectively. CONCLUSIONS: Since II values were less than 0.6 for both analytes, the reference values will be of little use. RCV might be preferred for better evaluation instead.


Assuntos
Coleta de Amostras Sanguíneas/métodos , Voluntários Saudáveis , Zinco/sangue , Adulto , Estudos de Coortes , Cobre/sangue , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Valores de Referência , Espectrofotometria Atômica
14.
World J Surg Oncol ; 17(1): 78, 2019 May 06.
Artigo em Inglês | MEDLINE | ID: mdl-31060563

RESUMO

BACKGROUND: Inconsistent results according to numerous studies that had investigated the association between serum zinc levels and lung cancer risk were reported. The aim of this study was to explore whether serum zinc levels were lower in lung cancer patients than that in controls. METHODS: We systematically retrieved the databases of PubMed, Wanfang, Cochrane, ScienceDirect website, CNKI, and SinoMed databases for comprehensive relevant studies published before December 2018 and conducted a meta-analysis. Standard mean differences (SMD) were pooled using a random effects model. RESULTS: Thirty-two articles were eligible to investigate the correlation between serum zinc levels and lung cancer risk, involving 2894 cases and 9419 controls. The pooled results showed sufficient evidence approving the association between serum zinc levels and lung cancer risk. And the serum zinc levels in lung cancer were significantly lower than that in controls (summary SMD = - 0.88, 95% confidence interval (CI) = - 0.94, - 0.82). Meanwhile, consistent results were obtained both in European populations and Asian populations. No publication bias was detected in our analysis. CONCLUSIONS: The present meta-analysis suggested that serum zinc levels were significantly lower in lung cancer patients than that in controls.


Assuntos
Neoplasias Pulmonares/sangue , Neoplasias Pulmonares/etiologia , Zinco/sangue , Estudos de Casos e Controles , Bases de Dados Factuais , Humanos , Estudos Observacionais como Assunto , Prognóstico , Fatores de Risco
15.
Theriogenology ; 133: 97-103, 2019 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-31078069

RESUMO

The role of oxidative stress in the control of female reproduction has not been fully elucidated in ruminants; however, it seems that antioxidants can make influence to the reproductive axis at different levels. Therefore, the aim of this study was to determine the relationship between antioxidant status and concentrations of trace minerals (chromium (Cr), copper (Cu), iron (Fe), and zinc (Zn)) with postpartum luteal activity and fertility in Holstein dairy cows. The cows (n = 100, a parity range of 2-5, and a body condition score (BCS) of 3.0 ±â€¯0.25, mean ±â€¯SEM) were assigned to the experiment at second week post calving. Ovarian follicular dynamics were monitored twice a week (from 3rd to 6th weeks post calving) by transrectal ultrasonography (US). Blood samples were collected twice weekly from the 3rd to the 6th weeks post calving at timed artificial insemination (TAI), and days 32 and 50 post AI to determine superoxide dismutase (SOD), glutathione peroxidase (GPX), total antioxidant capacity (TAC), and trace mineral concentrations. There were associations between plasma concentrations of SOD, GPX, and TAC with postpartum luteal activity (PLA, P = 0.01) and ovulation (P = 0.03). Mean plasma SOD and GPX activities and TAC levels (U/mL) were greater in cows with normal luteal activity (NLA) than prolonged luteal phase (PLP) and anovulation (AO) cows, as well as in ovulated compared to AO cows (P = 0.03). Pregnant cows had greater levels of SOD, GPX, and TAC (U/mL) at TAI than non-pregnant cows (P = 0.01). Plasma Cu and Zn concentrations increased in pregnant compared to non-pregnant cows at TAI. In conclusion, antioxidant levels and Cu and Zn concentrations were associated with PLA and fertility.


Assuntos
Aborto Animal , Antioxidantes/metabolismo , Corpo Lúteo/fisiologia , Oligoelementos/sangue , Animais , Bovinos , Cromo/sangue , Cobre/sangue , Corpo Lúteo/metabolismo , Feminino , Glutationa Peroxidase/sangue , Inseminação Artificial/veterinária , Ferro/sangue , Período Pós-Parto/metabolismo , Gravidez , Superóxido Dismutase/sangue , Zinco/sangue
16.
Nutrients ; 11(5)2019 May 08.
Artigo em Inglês | MEDLINE | ID: mdl-31071930

RESUMO

Zinc could have a protective role against type 2 diabetes mellitus (T2DM). This systematic review and meta-analysis aimed to evaluate the association between dietary, supplementary, and total zinc intake, as well as serum/plasma and whole blood zinc concentration, and risk of T2DM. Observational studies, conducted on cases of incident diabetes or T2DM patients and healthy subjects that reported a measure of association between zinc exposure and T2DM, were selected. Random effects meta-analyses were applied to obtain combined results. Stratified meta-analyses and meta-regressions were executed to assess sources of heterogeneity, as well as the impact of covariates on the findings. From 12,136 publications, 16 studies were selected. The odds ratio (OR) for T2DM comparing the highest versus lowest zinc intake from diet was 0.87 (95% CI: 0.78-0.98). Nevertheless, no association between supplementary or total zinc intake from both diet and supplementation, and T2DM was observed. A direct relationship was found between serum/plasma zinc levels and T2DM (OR = 1.64, 95% CI: 1.25-2.14). A moderately high dietary zinc intake, in relation to the Dietary Reference Intake, could reduce by 13% the risk of T2DM, and up to 41% in rural areas. Conversely, elevated serum/plasma zinc concentration was associated with an increased risk of T2DM by 64%, suggesting disturbances in zinc homeostasis.


Assuntos
Diabetes Mellitus Tipo 2/etiologia , Estado Nutricional , Zinco/administração & dosagem , Humanos , Razão de Chances , Fatores de Risco , Zinco/sangue
17.
Medicina (Kaunas) ; 55(5)2019 May 21.
Artigo em Inglês | MEDLINE | ID: mdl-31117252

RESUMO

Background and Objectives: Altered copper and zinc homeostasis may influence the antioxidant defense system and consequently lead to oxidative stress and associated complications in sickle cell disease (SCD) patients. Iron levels have been reported to increase in sickle cell patients due to frequent blood transfusion, chronic intravenous haemolysis and increased absorption of iron from the gastrointestinal tract. These elevated levels of iron may also lead to extensive oxidative damage. The current study evaluated serum levels of iron, copper and zinc in SCD patients and "healthy" controls. Materials and Methods: The study was a cross-sectional one, comprising 90 SCD patients with Haemoglobin SS and Haemoglobin SC genotypes and 50 HbAA "healthy" controls. Serum levels of iron, copper and zinc were measured using a Flame Atomic Absorption Spectrometer (Variant 240FS manufactured by VARIAN Australia Pty Ltd, VIC, Australia). Copper and zinc ratios were calculated and analyzed. Results: Serum levels of iron and copper were significantly elevated in the SCD patients, compared to their "healthy" counterparts (p < 0.001). These levels were further increased in patients with haemoglobin SS in vaso-occlusive crises (HbSS VOCs). Serum zinc levels were, however, significantly lower in the SCD patients, particularly during vaso-occlusion. The copper-to-zinc ratio was also found to be significantly higher in the SCD patients. Conclusion: Elevated copper-to-zinc ratio may be a biomarker of sickle cell oxidative stress and associated complications. The ratio may also be informative for the management of sickle cell oxidative burden. The significantly lower levels of zinc in the SCD patients may warrant zinc supplementation.


Assuntos
Anemia Falciforme/sangue , Cobre/análise , Ferro/análise , Zinco/análise , Adulto , Anemia Falciforme/complicações , Biomarcadores/análise , Biomarcadores/sangue , Cobre/sangue , Estudos Transversais , Feminino , Gana , Humanos , Ferro/sangue , Masculino , Pessoa de Meia-Idade , Estresse Oxidativo/fisiologia , Zinco/sangue
18.
Wei Sheng Yan Jiu ; 48(2): 238-243, 2019 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-31133101

RESUMO

OBJECTIVE: To investigate the distribution and changes of blood copper, zinc, calcium, magnesium and iron in Chinese rural children aged 6 to 12 years in 2002 and 2012. METHODS: A total of 3420 blood samples were randomly selected from the China Nutrition and Health Survey 2002 and 2012. Metal concentrations in whole blood were determined by inductively coupled mass spectrometry(ICP-MS) after the dilution of 0. 5%(V/V) HNO_3 and 0. 05%(V/V) Triton-X-100. The changes between 2002 and 2012 of blood five elements concentration in children of 6-12 years in rural China were compared. RESULTS: The median of blood levels of copper, zinc, calcium, magnesium and iron in 2002 were 1. 02(95% CI 0. 79-1. 32), 4. 88(95% CI 3. 17-6. 77), 58. 59(95% CI 44. 97-74. 24), 41. 56(95% CI 33. 01-52. 28) and 423. 21(95% CI 338. 83-540. 69) mg/L, respectively. And in 2012, the median values were 1. 00(95% CI 0. 75-1. 31), 5. 12(95% CI 3. 17-7. 37), 63. 36(95% CI 41. 14-79. 16), 41. 78(95% CI 30. 89-52. 53) and 422. 06(95% CI 297. 83-522. 56) mg/L, respectively. There were significant differences in blood copper, zinc, calcium and iron levels in general, gender and age. There were also significant area differences in the distribution of copper, zinc, calcium, magnesium and iron, but the changes were opposite. CONCLUSION: In 2002 and 2012, there were differences in the blood distribution of Cu, Zn, Ca, Mg and Fe of children aged 6-12 in rural China in terms of general, gender, age and area.


Assuntos
Cálcio/sangue , Cobre/sangue , Ferro/sangue , Magnésio/sangue , Zinco/sangue , Criança , China , Feminino , Hemoglobinas/metabolismo , Humanos , Masculino , Espectrofotometria Atômica , Oligoelementos
19.
J Trace Elem Med Biol ; 54: 221-225, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31109616

RESUMO

In order to assess the individual trace element status of humans for either medical or scientific purposes, amongst others, blood serum levels are determined. Furthermore, animal models are used to study interactions of trace elements. Most published methods require larger amounts (500-1000 µL) of serum to achieve a reliable determination of multiple trace elements. However, oftentimes, these amounts of serum cannot be dedicated to a single analysis and the amount available for TE-determination is much lower. Therefore, a published ICP-MS/MS method for trace element determination in serum was miniaturized, optimized and validated for the measurement of Mn, Fe, Cu Zn, I and Se in as little as 50 µL of human and murine serum and is presented in this work. For validation, recoveries of multiple LOTs and levels from commercially available human reference serum samples were determined, intra- and inter-day variations were assessed and limits of detection and quantification determined. It is shown, that the method is capable of giving accurate and reproducible results for all six elements within the relevant concentration ranges for samples from humans living in central Europe as well as from laboratory mice. As a highlight, the achieved limits of detection and quantification for Mn were found to be at 0.02 µg/L serum and 0.05 µg/L serum, respectively, while using an alkaline diluent for the parallel determination of iodine.


Assuntos
Espectrometria de Massas em Tandem/métodos , Oligoelementos/sangue , Animais , Cobre/sangue , Humanos , Iodo/sangue , Ferro/sangue , Manganês/sangue , Camundongos , Selênio/sangue , Zinco/sangue
20.
Medicine (Baltimore) ; 98(15): e15172, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30985701

RESUMO

The incidence of inflammatory bowel disease (IBD) is increasing and the pathogenesis is still not completely understood. Micronutrients like vitamin D [25 (OH)D] and zinc play an important role in enzyme activities and the immune system. As the 25 (OH)D-receptor has been shown to be downregulated in patients with IBD, 25 (OH)D may emerge as a predictive marker for disease improvement. Studies on relationship of both micronutrients in IBD patients are lacking.We retrospectively evaluated serum levels of 25(OH)D and zinc together with baseline characteristics of 232 IBD patients. Uni- and multivariate analyses were performed for association between serum levels of 25(OH)D and zinc with clinical and deep remission (CR and DR).155 Crohn's disease (CD) and 77 ulcerative colitis (UC) patients were included. 54% (n = 125) and 6% (n = 14) of IBD patients showed deficient serum 25(OH)D levels below 20 ng/mL and zinc levels below 7 µmol/L. Serum 25(OH)D levels were significantly higher in IBD patients with CR (P = .02) and DR (P < .001) but not serum zinc levels, respectively. Serum 25(OH)D levels (P = .008), anti-tumor-necrosis-factor-α-trough-concentration (anti-TNF-α-TC) (P = .02) and CRP level (P = .02) were independently associated with CR in CD patients. Serum 25(OH)D threshold of 19 ng/mL discriminated CD patients with or without CR, having an area under the receiver operating curve analysis (AUROC) of 0.77 [95%-confidence interval (CI): 0.68-0.85]. In multivariate analysis serum 25(OH)D levels (P = .04) and anti-TNF-α-TC (P = .04) were associated with DR in CD patients. Serum 25(OH)D threshold of 26 ng/mL discriminated CD patients with or without DR, having an AUROC of 0.75 (95%-CI: 0.68-0.83).Serum 25(OH)D (P = .04) and fecal calprotectin levels (P = .04) were independently correlated with CR in UC patients. Serum 25(OH)D threshold of 32 ng/mL discriminated UC patients in CR with an AUROC of 0.83 (95%-CI: 0.71-0.95). Zinc levels did not correlate with disease activity status in CD or UC patients either.In conclusion, beside CRP and fecal calprotectin, serum 25(OH)D levels, but not serum zinc levels, may be an additional useful and noninvasive marker for characterizing different disease activity status of IBD patients. Measurement of serum 25(OH)D in IBD patients may be warranted. 25(OH)D supplementation in deficient IBD patients is recommended.


Assuntos
Colite Ulcerativa/sangue , Doença de Crohn/sangue , Vitamina D/sangue , Zinco/sangue , Adulto , Biomarcadores/sangue , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Estudos Retrospectivos , Índice de Gravidade de Doença
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