Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 1.895
Filtrar
1.
Pol J Vet Sci ; 24(3): 445-450, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34730306

RESUMO

Helicobacter species have been reported in animals, some of which are of zoonotic importance. This study aimed to detect Helicobacter species among human and animal samples using conventional PCR assays and to identify their zoonotic potentials. Helicobacter species was identified in human and animal samples by genus-specific PCR assays and phylogenetic analysis of partial sequencing of the 16S ribosomal RNA gene. The results revealed that Helicobacter species DNA was detected in 13 of 29 (44.83%) of the human samples. H. pylori was identified in 2 (15.38%), and H. bovis was detected in 4 (30.77%), whereas 7 (53.85%) were unidentified. H. bovis and H. heilmannii were prevalent among the animal samples. Phylogenetic analysis revealed bootstrapping of sequences with H. cinaedi in camel, H. rappini in sheep and humans, and Wollinella succinogenes in humans. In conclusion, the occurrence of non-H. pylori infections among human and animal samples suggested zoonotic potentials.


Assuntos
Doenças do Gato/microbiologia , Doenças do Cão/microbiologia , Infecções por Helicobacter/veterinária , Helicobacter/genética , Helicobacter/isolamento & purificação , Zoonoses/microbiologia , Animais , Doenças do Gato/epidemiologia , Gatos , DNA Bacteriano/genética , Doenças do Cão/epidemiologia , Cães , Fezes/microbiologia , Genoma Bacteriano , Helicobacter/classificação , Infecções por Helicobacter/diagnóstico , Infecções por Helicobacter/microbiologia , Hospitais Veterinários , Humanos , Filogenia , Reação em Cadeia da Polimerase , Saliva/microbiologia , Zoonoses/epidemiologia
2.
Epidemiol Infect ; 149: e234, 2021 10 27.
Artigo em Inglês | MEDLINE | ID: mdl-34702393

RESUMO

Poultry contact is a risk factor for zoonotic transmission of non-typhoidal Salmonella spp. Salmonella illness outbreaks in the United States are identified by PulseNet, the national laboratory network for enteric disease surveillance. During 2020, PulseNet observed a 25% decline in the number of Salmonella clinical isolates uploaded by state and local health departments. However, 1722 outbreak-associated Salmonella illnesses resulting from 12 Salmonella serotypes were linked to contact with privately owned poultry, an increase from all previous years. This report highlights the need for continued efforts to prevent backyard poultry-associated outbreaks of Salmonella as ownership increases in the United States.


Assuntos
COVID-19/epidemiologia , Surtos de Doenças/estatística & dados numéricos , Aves Domésticas/microbiologia , Infecções por Salmonella/epidemiologia , Zoonoses/epidemiologia , Animais , Humanos , SARS-CoV-2 , Salmonella/isolamento & purificação , Infecções por Salmonella/microbiologia , Infecções por Salmonella/transmissão , Sorogrupo , Estados Unidos/epidemiologia , Zoonoses/microbiologia , Zoonoses/transmissão
3.
J Infect Dev Ctries ; 15(8): 1104-1106, 2021 08 31.
Artigo em Inglês | MEDLINE | ID: mdl-34516417

RESUMO

Brucellosis is a common zoonotic infection. Brucellosis typically presents with fever, weakness, night sweats, and arthralgias. Symptoms associated with Coronavirus Disease 2019 (COVID-19) and infection with Brucella spp. are similar to one another, which may lead to delayed diagnosis of the latter condition. There are no previous reports of brucellosis in a patient previously diagnosed with COVID-19. We present here the case of a 20-year-old male who we diagnosed with brucellosis after joint pains and fever that persisted after resolution of COVID-19.


Assuntos
Brucelose/diagnóstico , COVID-19/complicações , Coinfecção/diagnóstico , Zoonoses/diagnóstico , Animais , Artralgia/microbiologia , Brucelose/fisiopatologia , COVID-19/diagnóstico por imagem , Coinfecção/microbiologia , Coinfecção/virologia , Diagnóstico Diferencial , Febre/microbiologia , Humanos , Masculino , Tomografia Computadorizada por Raios X , Adulto Jovem , Zoonoses/microbiologia
4.
Sci Rep ; 11(1): 17467, 2021 09 01.
Artigo em Inglês | MEDLINE | ID: mdl-34471179

RESUMO

Intestinal parasitic infections have high prevalence rate in many regions especially in developing countries. The aim of this study was to determine the presence and genotype/subtype of some intestinal protozoa in livestock in Iran. Stool samples were collected from cattle, sheep, chickens, and horses. The presence of targeted parasites was evaluated using real-time PCR. Genotyping/subtyping of positive samples was characterized using sequencing of the ITS and barcoding region, respectively. Blastocystis sp., 27.7% (48/173) and Enterocytozoon bieneusi 26.0% (45/173) were the most frequent protozoa followed by Encephalitozoon spp., 0.57% (1/173). Cryptosporidium spp. were not detected among samples. Encephalitozoon spp., was detected only in chickens 2.2% (1/45). A statistically correlation was seen between animals and the prevalence of targeted protozoa. E. bieneusi genotypes I (9/38; 23.68%), BEB6 (22/38; 57.89%), D (6/38; 15.79%), and horse1 (1/38; 2.63%) were detected among samples. A statistically significant correlation was seen between the genotypes and animals (P ≤ 0.05). Blastocystis sp., ST1 (1/45; 2.22%), ST5 3/45; 6.66%), ST7 (1/45; 2.22%), ST10 (24/45; 53.33%), and ST14 (16/45; 35.55%) were characterized among samples. There was no significant correlation between certain subtypes and animals (P = 0.173). The presence of zoonotic potential genotypes of E. bieneusi in animals and zoonotic potential subtypes ST1 and ST7 among our samples provide a clue about the transmission dynamic of E. bieneusi and Blastocystis sp. between animals-animals and humans-animals.


Assuntos
Blastocystis/fisiologia , Encephalitozoon/fisiologia , Enterocytozoon/fisiologia , Zoonoses/epidemiologia , Animais , Blastocystis/genética , Infecções por Blastocystis/epidemiologia , Infecções por Blastocystis/parasitologia , Bovinos , Galinhas , Encephalitozoon/genética , Encefalitozoonose/epidemiologia , Encefalitozoonose/microbiologia , Enterocytozoon/genética , Genótipo , Cavalos , Irã (Geográfico)/epidemiologia , Gado , Microsporidiose/epidemiologia , Microsporidiose/microbiologia , Epidemiologia Molecular , Filogenia , Prevalência , Ovinos , Zoonoses/microbiologia , Zoonoses/parasitologia
6.
PLoS Negl Trop Dis ; 15(8): e0009693, 2021 08.
Artigo em Inglês | MEDLINE | ID: mdl-34460830

RESUMO

BACKGROUND: Sporotrichosis is a worldwide subcutaneous mycosis caused by Sporothrix spp. In the past, this infection was associated with armadillo hunting, horticulturists, miners, and gardeners, being considered an implantation mycosis acquired by plant debris injury. Nevertheless, since the late nineties, it has been considered a zoonotic disease in Brazil. Here we report a case series of 121 patients with cat-transmitted sporotrichosis seen in Northeast Brazil. METHODOLOGY/PRINCIPAL FINDINGS: Patient's demographic, clinical data, and length of treatment were recorded. In addition, a mycological examination and further PCR confirmation of species identification were performed. One hundred and twenty two patients were diagnosed with subcutaneous sporotrichosis from October 2016 to December 2019, while PCR revealed that 71 of them were due to S. brasiliensis. The majority of the individuals were female (n = 86; 70.5%). Patient's age ranged from 5 to 87 years old. The clinical forms found were lymphocutaneous (58.2%) and fixed cutaneous (39.4%). Interestingly, 115 patients reported previous contact with cats diagnosed with sporotrichosis. Patients were successfully treated with itraconazole and potassium iodide. CONCLUSIONS/SIGNIFICANCE: Our study adds important contributions for the investigation of the spread of cat-transmitted subcutaneous sporotrichosis in Brazil, specifically towards the Northeast region of a continental-size country. It will also help clinicians to be aware of the existence and importance to accurately diagnose sporotrichosis and treat patients with this infectious disease in the lowest income region of Brazil.


Assuntos
Sporothrix/fisiologia , Esporotricose/transmissão , Zoonoses/transmissão , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Animais , Antifúngicos/uso terapêutico , Brasil , Doenças do Gato/microbiologia , Gatos , Criança , Pré-Escolar , Feminino , Humanos , Itraconazol/uso terapêutico , Masculino , Pessoa de Meia-Idade , Sporothrix/efeitos dos fármacos , Sporothrix/genética , Sporothrix/isolamento & purificação , Esporotricose/tratamento farmacológico , Esporotricose/microbiologia , Adulto Jovem , Zoonoses/tratamento farmacológico , Zoonoses/microbiologia
7.
PLoS One ; 16(7): e0254530, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34283853

RESUMO

Brucellosis and Q fever are neglected zoonoses of global health importance, with unknown true prevalence in occupationally vulnerable settings, partly due to misdiagnosis for other febrile conditions and poor access to primary health care. We examined the seroprevalence of these diseases and associated factors amongst pastoralists and their cattle in Sokoto State, a hub of cattle and pastoral populations in Nigeria. Serum samples randomly collected from 137 pastoralists and 366 cattle from 27 herds in three selected Local Government Areas (LGAs) in the state were analysed for antibodies to Brucella abortus using Rose Bengal Plate Test (RBT) and competitive Enzyme-Linked Immunosorbent Assay (cELISA) as well as antibodies to Coxiella burnetti using indirect ELISA. Consenting pastoralists' knowledge, perception and practices about the diseases were assessed using a semi-structured questionnaire. Data were analysed using descriptive statistics and bivariate analysis at p ≤ 0.05 level of significance. Brucellosis adjusted individual seroprevalence were 0.83% (95%CI: 0.04-4.59%) and 0% among pastoralists; 2.28% (95%CI: 1.16-4.43%) and 5.70% (95%CI: 3.68-8.74%) in cattle by RBT and cELISA, respectively. Adjusted herd-level seroprevalence for brucellosis were 23.20% (95%CI: 11.07-42.54%) and 42.00% (95%CI: 25.27-61.11%) by RBT and cELISA, respectively. For Q fever, higher seroprevalence of 62.57% (95%CI: 54.04-70.46%) and 2.98% (95%CI: 1.57-5.58%) were recorded amongst the pastoralists and their cattle, respectively. with adjusted herd-level seroprevalence of 40.36% (95%CI: 22.57-63.17%). The LGAs of sampling were significantly (OR: 0.2; 95%CI: 0.02-1.00) associated with Q fever infection, though marginal. The majority of the pastoralists had poor knowledge, perception and practices towards the diseases. This is the first study establishing the presence of brucellosis and Q fever at the human-animal interface in Sokoto State, Nigeria. The pastoralists' poor knowledge, perception and practices about these diseases are worrisome and are important factors for consideration in disease control.


Assuntos
Brucelose/sangue , Febre Q/sangue , Estudos Soroepidemiológicos , Zoonoses/sangue , Criação de Animais Domésticos , Animais , Brucella abortus/isolamento & purificação , Brucella abortus/patogenicidade , Brucelose/epidemiologia , Brucelose/microbiologia , Bovinos , Ensaio de Imunoadsorção Enzimática , Cabras/sangue , Cabras/microbiologia , Humanos , Nigéria/epidemiologia , Febre Q/epidemiologia , Febre Q/microbiologia , Fatores de Risco , Zoonoses/epidemiologia , Zoonoses/microbiologia , Zoonoses/transmissão
8.
PLoS Negl Trop Dis ; 15(7): e0009520, 2021 07.
Artigo em Inglês | MEDLINE | ID: mdl-34264939

RESUMO

Q fever is a worldwide zoonosis caused by Coxiella burnetii (Cb). From January 2018 to November 2019, plasma samples from 2,382 patients with acute fever of unknown cause at a hospital in Zhuhai city of China were tested using metagenomic next-generation sequencing (mNGS). Of those tested, 138 patients (5.8%) were diagnosed with Q fever based on the presence of Cb genomic DNA detected by mNGS. Among these, 78 cases (56.5%) presented from Nov 2018 to Mar 2019, suggesting an outbreak of Q fever. 55 cases with detailed clinical information that occurred during the outbreak period were used for further analysis. The vast majority of plasma samples from those Cb-mNGS-positive patients were positive in a Cb-specific quantitative polymerase chain reaction (n = 38) and/or indirect immunofluorescence assay (n = 26). Mobile phone tracing data was used to define the area of infection during the outbreak. This suggested the probable infection source was Cb-infected goats and cattle at the only official authorized slaughterhouse in Zhuhai city. Phylogenic analysis based on genomic sequences indicated Cb strains identified in the patients, goat and cattle were formed a single branch, most closely related to the genomic group of Cb dominated by strains isolated from goats. Our study demonstrates Q fever was epidemic in 2018-2019 in Zhuhai city, and this is the first confirmed epidemic of Q fever in a contemporary city in China.


Assuntos
Coxiella burnetii/isolamento & purificação , Febre Q/microbiologia , Adulto , Animais , Bovinos , Doenças dos Bovinos/epidemiologia , Doenças dos Bovinos/microbiologia , Doenças dos Bovinos/transmissão , China/epidemiologia , Coxiella burnetii/classificação , Coxiella burnetii/genética , Surtos de Doenças , Feminino , Doenças das Cabras/epidemiologia , Doenças das Cabras/microbiologia , Doenças das Cabras/transmissão , Cabras , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Masculino , Metagenômica , Pessoa de Meia-Idade , Filogenia , Febre Q/diagnóstico , Febre Q/epidemiologia , Febre Q/transmissão , Adulto Jovem , Zoonoses/diagnóstico , Zoonoses/epidemiologia , Zoonoses/microbiologia , Zoonoses/transmissão
9.
Epidemiol Infect ; 149: e124, 2021 05 06.
Artigo em Inglês | MEDLINE | ID: mdl-33955833

RESUMO

In August 2017, a cluster of four persons infected with genetically related strains of Shiga toxin-producing Escherichia coli (STEC) O157:H7 was identified. These strains possessed the Shiga toxin (stx) subtype stx2a, a toxin type known to be associated with severe clinical outcome. One person died after developing haemolytic uraemic syndrome. Interviews with cases revealed that three of the cases had been exposed to dogs fed on a raw meat-based diet (RMBD), specifically tripe. In two cases, the tripe had been purchased from the same supplier. Sampling and microbiological screening of raw pet food was undertaken and indicated the presence of STEC in the products. STEC was isolated from one sample of raw tripe but was different from the strain causing illness in humans. Nevertheless, the detection of STEC in the tripe provided evidence that raw pet food was a potential source of human STEC infection during this outbreak. This adds to the evidence of raw pet food as a risk factor for zoonotic transmission of gastrointestinal pathogens, which is widely accepted for Salmonella, Listeria and Campylobacter spp. Feeding RMBD to companion animals has recently increased in popularity due to the belief that they provide health benefits to animals. Although still rare, an increase in STEC cases reporting exposure to RMBDs was detected in 2017. There has also been an increased frequency of raw pet food incidents in 2017, suggesting an increasing trend in potential risk to humans from raw pet food. Recommendations to reduce the risk of infection included improved awareness of risk and promotion of good hygiene practices among the public when handling raw pet food.


Assuntos
Infecções por Escherichia coli/microbiologia , Escherichia coli O157/isolamento & purificação , Animais de Estimação , Alimentos Crus/microbiologia , Animais , Dieta/veterinária , Surtos de Doenças , Cães , Infecções por Escherichia coli/epidemiologia , Infecções por Escherichia coli/transmissão , Escherichia coli O157/genética , Manipulação de Alimentos , Microbiologia de Alimentos , Síndrome Hemolítico-Urêmica/epidemiologia , Síndrome Hemolítico-Urêmica/microbiologia , Humanos , Carne/microbiologia , Toxina Shiga/genética , Zoonoses/epidemiologia , Zoonoses/microbiologia , Zoonoses/transmissão
10.
Med Mycol ; 59(9): 934-938, 2021 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-33998652

RESUMO

Enterocytozoon bieneusi, an important microsporidian fungus, causes chronic diarrhea in humans and animals worldwide. Out of the 502 fecal samples from wild boars, 13 were positive for the E. bieneusi internal transcribed spacer region, with a prevalence of 2.6%. Six E. bieneusi genotypes, D, EbpC, and four novel KWB1-KWB4, were identified with zoonotic potential. Genotypes D (subgroup 1a) and EbpC (subgroup 1d) were first reported in Korean swine and Korea, respectively; KWB1-KWB4 (subgroup 1e) were most prevalent in this study. Because zoonotic genotypes have been identified, E. bieneusi transmission through wild boars must be closely monitored for proper prevention and treatment, despite their low prevalence. LAY SUMMARY: Enterocytozoon bieneusi is an important microsporidian fungus. Its sequences from wild boars were identified with zoonotic potential. Genotypes D and EbpC were first reported in Korean swine and Korea, respectively. E. bieneusi should be closely monitored to properly prevent and treat animals.


Assuntos
Enterocytozoon/genética , Fezes/microbiologia , Microsporidiose/microbiologia , Sus scrofa/microbiologia , Doenças dos Suínos/microbiologia , Zoonoses/microbiologia , Animais , Animais Selvagens/microbiologia , Variação Genética , Genótipo , Geografia , Masculino , Microsporidiose/genética , Filogenia , Prevalência , República da Coreia , Suínos , Doenças dos Suínos/genética
11.
PLoS One ; 16(5): e0251901, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34038443

RESUMO

Leptospirosis is an emerging worldwide zoonotic disease, but the general biology of the causative agents is still poorly understood. Humans are an occasional host. The main risk factors are water-associated exposure during professional or recreational activities or during outbreaks in endemic areas. Detecting the presence of pathogenic bacteria in aquatic environments and their capacity to resist various inactivation processes are research fields that need to be further developed. In addition, the methods used for detecting and enumerating Leptospira still need to be improved. We aimed to describe a new quantitative polymerase chain reaction coupled to propidium monoazide treatment (PMAqPCR) that targets not only total Leptospira but also discriminates pathogenic from non-pathogenic Leptospira while also addressing PCR inhibitors, a frequently encountered problem when studying environmental water. In a second step, the killing efficiency of Leptospira to different treatments was tested and PMAqPCR compared to culture-based enumeration. This provided information about the effects of temperature, as well as ultraviolet and chlorine disinfection, that are both related to water treatment processes, in particular for the production of drinking water, on the persistence of both saprophytic and pathogenic Leptospira. Finally, PMAqPCR was used for the detection of Leptospira in freshwater samples for a proof-of-concept. In conclusion, our method could be used for routine freshwater monitoring and allows better evaluation of the presence of Leptospira, allowing evaluation of the bacterial dynamics in a designated area or assessment of the efficacy of water disinfection processes.


Assuntos
DNA Bacteriano/isolamento & purificação , Desinfecção , Leptospira/isolamento & purificação , Leptospirose/microbiologia , Animais , Monitoramento Ambiental , Água Doce/microbiologia , Humanos , Leptospira/patogenicidade , Leptospirose/diagnóstico , Leptospirose/genética , Reação em Cadeia da Polimerase em Tempo Real , Água , Microbiologia da Água , Zoonoses/microbiologia
12.
Science ; 372(6541): 450-455, 2021 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-33926935
13.
mBio ; 12(2)2021 04 13.
Artigo em Inglês | MEDLINE | ID: mdl-33849979

RESUMO

Animals that are competent reservoirs of zoonotic pathogens commonly suffer little morbidity from the infections. To investigate mechanisms of this tolerance of infection, we used single-dose lipopolysaccharide (LPS) as an experimental model of inflammation and compared the responses of two rodents: Peromyscus leucopus, the white-footed deermouse and reservoir for the agents of Lyme disease and other zoonoses, and the house mouse Mus musculus Four hours after injection with LPS or saline, blood, spleen, and liver samples were collected and subjected to transcriptome sequencing (RNA-seq), metabolomics, and specific reverse transcriptase quantitative PCR (RT-qPCR). Differential expression analysis was at the gene, pathway, and network levels. LPS-treated deermice showed signs of sickness similar to those of exposed mice and had similar increases in corticosterone levels and expression of interleukin 6 (IL-6), tumor necrosis factor, IL-1ß, and C-reactive protein. By network analysis, the M. musculus response to LPS was characterized as cytokine associated, while the P. leucopus response was dominated by neutrophil activity terms. In addition, dichotomies in the expression levels of arginase 1 and nitric oxide synthase 2 and of IL-10 and IL-12 were consistent with type M1 macrophage responses in mice and type M2 responses in deermice. Analysis of metabolites in plasma and RNA in organs revealed species differences in tryptophan metabolism. Two genes in particular signified the different phenotypes of deermice and mice: the Slpi and Ibsp genes. Key RNA-seq findings for P. leucopus were replicated in older animals, in a systemic bacterial infection, and with cultivated fibroblasts. The findings indicate that P. leucopus possesses several adaptive traits to moderate inflammation in its balancing of infection resistance and tolerance.IMPORTANCE Animals that are natural carriers of pathogens that cause human diseases commonly manifest little or no sickness as a consequence of infection. Examples include the deermouse, Peromyscus leucopus, which is a reservoir for Lyme disease and several other disease agents in North America, and some types of bats, which are carriers of viruses with pathogenicity for humans. Mechanisms of this phenomenon of infection tolerance and entailed trade-off costs are poorly understood. Using a single injection of lipopolysaccharide (LPS) endotoxin as a proxy for infection, we found that deermice differed from the mouse (Mus musculus) in responses to LPS in several diverse pathways, including innate immunity, oxidative stress, and metabolism. Features distinguishing the deermice cumulatively would moderate downstream ill effects of LPS. Insights gained from the P. leucopus model in the laboratory have implications for studying infection tolerance in other important reservoir species, including bats and other types of wildlife.


Assuntos
Reservatórios de Doenças/microbiologia , Endotoxinas/administração & dosagem , Inflamação/genética , Peromyscus/microbiologia , Zoonoses/imunologia , Zoonoses/microbiologia , Animais , Suscetibilidade a Doenças/etiologia , Suscetibilidade a Doenças/imunologia , Endotoxinas/imunologia , Feminino , Perfilação da Expressão Gênica , Inflamação/imunologia , Doença de Lyme/microbiologia , Masculino , Metabolômica , Camundongos , Camundongos Endogâmicos BALB C , Peromyscus/imunologia , Análise de Sequência de RNA
14.
Trop Biomed ; 38(1): 130-134, 2021 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-33797535

RESUMO

Tularemia is a zoonotic disease and endemic in the northern hemisphere. The aim of this study was to evaluate the epidemiological, clinical and laboratory characteristics of tularemia patients, and to re-analyze their lymphadenopathy during the follow-up. The patients who were diagnosed with tularemia were reviewed. They were invited for the long term, physical and radiological evaluations. 69.8% patients had lived in rural areas. 54.7% patients were associated with animal husbandry, the 18.9% had contact with rodents. The most common form was the glandular type (62.3%). The frequency of granulomatous lymphadenitis was significantly higher in patients diagnosed later than 30 days from the onset of symptoms. Lymphadenopathy was undetectable in 61.5% patients, its severity was reduced in 38.4% patients compared to its state at the admission. In rural areas, avoiding contact with wild animals can ensure the protection from the pathogen. Public communities should be made aware of the disease.


Assuntos
Tularemia/diagnóstico , Adolescente , Adulto , Animais , Feminino , Humanos , Linfadenite/microbiologia , Masculino , Tularemia/epidemiologia , Tularemia/patologia , Turquia , Adulto Jovem , Zoonoses/microbiologia
15.
Trends Parasitol ; 37(6): 465-467, 2021 06.
Artigo em Inglês | MEDLINE | ID: mdl-33858779

RESUMO

Global emergence of vector-borne and zoonotic diseases presents a rapidly growing 'wicked' problem. We outline the need for a transdisciplinary research program that is grounded in ecological and evolutionary theory but integrates fundamentally with research perspectives spanning the health, social, and natural sciences.


Assuntos
Doenças Transmissíveis Emergentes/prevenção & controle , Doenças Transmissíveis Emergentes/transmissão , Pesquisa/tendências , Zoonoses/microbiologia , Animais , Doença de Lyme/microbiologia , Doença de Lyme/transmissão , Doenças Transmitidas por Vetores/microbiologia , Doenças Transmitidas por Vetores/transmissão , Zoonoses/parasitologia , Zoonoses/transmissão , Zoonoses/virologia
16.
Mycoses ; 64(11): 1378-1386, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33864711

RESUMO

BACKGROUND: Species from the Trichophyton benhamiae complex are mostly zoophilic dermatophytes which cause inflammatory dermatophytosis in animals and humans worldwide. OBJECTIVES: This study was purposed to (a) to identify 169 reference and clinical dermatophyte strains from the T benhamiae complex species by molecular method and adhering to the newest taxonomy in the complex (b) to evaluate the in vitro antifungal susceptibility profile of these strains against eight common and new antifungal agents that may be used for the treatment of dermatophytosis. METHODS: All isolates, mainly originated from Europe but also from Iran, Japan and USA, were subjected to ITS-rDNA sequencing. The in vitro antifungal susceptibility profiles of eight common and new antifungal drugs against the isolates were determined by CLSI M38-A2 protocol and according to microdilution method. RESULTS: Based on the ITS-rDNA sequencing, T benhamiae was the dominant species (n = 102), followed by T europaeum (n = 29), T erinacei (n = 23), T japonicum (n = 10), Trichophyton sp (n = 4) and T eriotrephon (n = 1). MIC ranges across all isolates were as follows: luliconazole: 0.0002-0.002 µg/ml, terbinafine: 0.008-0.125 µg/ml, efinaconazole: 0.008-0.125 µg/ml, ciclopirox olamine: 0.03-0.5 µg/ml, itraconazole: 0.06-2 µg/ml, griseofulvin: 0.25-4 µg/ml, amorolfine hydrochloride: 0.125-4 µg/ml and tavaborole: 1-16 µg/ml. CONCLUSION: Luliconazole, efinaconazole and terbinafine were the most potent antifungals against T benhamiae complex isolates, regardless of the geographic locations where strains were isolated. These data might help dermatologists to develop effective therapies for successful treatment of infections due to T benhamiae complex species.


Assuntos
Antifúngicos/farmacologia , Arthrodermataceae/efeitos dos fármacos , Tinha/microbiologia , Zoonoses/microbiologia , Animais , Antifúngicos/uso terapêutico , Arthrodermataceae/classificação , Arthrodermataceae/genética , Arthrodermataceae/isolamento & purificação , Europa (Continente) , Humanos , Irã (Geográfico) , Japão , Tinha/tratamento farmacológico , Estados Unidos , Zoonoses/tratamento farmacológico
17.
PLoS Negl Trop Dis ; 15(3): e0009272, 2021 03.
Artigo em Inglês | MEDLINE | ID: mdl-33735202

RESUMO

Leptospirosis is a ubiquitous zoonotic disease and a major clinical challenge owing to the multitude of clinical presentations and manifestations that are possibly attributable to the diversity of Leptospira, the understanding of which is key to study the epidemiology of this emerging global disease threat. Sri Lanka is a hotspot for leptospirosis with high levels of endemicity as well as annual epidemics. We carried out a prospective study of Leptospira diversity in Sri Lanka, covering the full range of climatic zones, geography, and clinical severity. Samples were collected for leptospiral culture from 1,192 patients from 15 of 25 districts in Sri Lanka over two and half years. Twenty-five isolates belonging to four pathogenic Leptospira species were identified: L. interrogans, L. borgpetersenii, L. weilii, and L. kirschneri. At least six serogroups were identified among the isolates: Autumnalis (6), Pyrogenes (4), Icterohaemorrhagiae (2), Celledoni (1), Grippotyphosa (2) and Bataviae (1). Seven isolates did not agglutinate using available antisera panels, suggesting new serogroups. Isolates were sequenced using an Illumina platform. These data add 25 new core genome sequence types and were clustered in 15 clonal groups, including 12 new clonal groups. L. borgpetersenii was found only in the dry zone and L. weilii only in the wet zone. Acute kidney injury and cardiovascular involvement were seen only with L. interrogans infections. Thrombocytopenia and liver impairment were seen in both L. interrogans and L. borgpetersenii infections. The inadequate sensitivity of culture isolation to identify infecting Leptospira species underscores the need for culture-independent typing methods for Leptospira.


Assuntos
Técnicas de Tipagem Bacteriana/métodos , Leptospira/classificação , Leptospira/isolamento & purificação , Leptospirose/epidemiologia , Injúria Renal Aguda/microbiologia , Adulto , Testes de Aglutinação , Animais , Doenças Cardiovasculares/microbiologia , DNA Bacteriano/genética , Epidemias , Feminino , Geografia , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Leptospira/genética , Leptospirose/microbiologia , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Estudos Prospectivos , Sri Lanka/epidemiologia , Zoonoses/diagnóstico , Zoonoses/epidemiologia , Zoonoses/microbiologia
18.
J Med Microbiol ; 70(3)2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33750514

RESUMO

Bacteria of the genus Streptococcus, earlier considered typically animal, currently have also been causing infections in humans. It is necessary to make clinicians aware of the emergence of new species that may cause the development of human diseases. There is an increasing frequency of isolation of streptococci such as S. suis, S. dysgalactiae, S. iniae and S. equi from people. Isolation of Streptococcus bovis/Streptococcus equinus complex bacteria has also been reported. The streptococcal species described in this review are gaining new properties and virulence factors by which they can thrive in new environments. It shows the potential of these bacteria to changes in the genome and the settlement of new hosts. Information is presented on clinical cases that concern streptococcus species belonging to the groups Bovis, Pyogenic and Suis. We also present the antibiotic resistance profiles of these bacteria. The emerging resistance to ß-lactams has been reported. In this review, the classification, clinical characteristics and antibiotic resistance of groups and species of streptococci considered as animal pathogens are summarized.


Assuntos
Farmacorresistência Bacteriana , Infecções Estreptocócicas/microbiologia , Streptococcus/fisiologia , Streptococcus/patogenicidade , Zoonoses/microbiologia , Animais , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Humanos , Infecções Estreptocócicas/tratamento farmacológico , Infecções Estreptocócicas/transmissão , Streptococcus/classificação , Streptococcus/efeitos dos fármacos , Virulência , Zoonoses/tratamento farmacológico , Zoonoses/transmissão
19.
PLoS One ; 16(3): e0248244, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33684147

RESUMO

Rodents are reservoirs of numerous zoonotic diseases caused by bacteria, protozoans, or viruses. In Gabon, the circulation and maintenance of rodent-borne zoonotic infectious agents are poorly studied and are often limited to one type of pathogen. Among the three existing studies on this topic, two are focused on a zoonotic virus, and the third is focused on rodent Plasmodium. In this study, we searched for a wide range of bacteria, protozoa and viruses in different organs of rodents from the town of Franceville in Gabon. Samples from one hundred and ninety-eight (198) small mammals captured, including two invasive rodent species, five native rodent species and 19 shrews belonging to the Soricidae family, were screened. The investigated pathogens were bacteria from the Rickettsiaceae and Anaplasmataceae families, Mycoplasma spp., Bartonella spp., Borrelia spp., Orientia spp., Occidentia spp., Leptospira spp., Streptobacillus moniliformis, Coxiella burnetii, and Yersinia pestis; parasites from class Kinetoplastida spp. (Leishmania spp., Trypanosoma spp.), Piroplasmidae spp., and Toxoplasma gondii; and viruses from Paramyxoviridae, Hantaviridae, Flaviviridae and Mammarenavirus spp. We identified the following pathogenic bacteria: Anaplasma spp. (8.1%; 16/198), Bartonella spp. (6.6%; 13/198), Coxiella spp. (5.1%; 10/198) and Leptospira spp. (3.5%; 7/198); and protozoans: Piroplasma sp. (1%; 2/198), Toxoplasma gondii (0.5%; 1/198), and Trypanosoma sp. (7%; 14/198). None of the targeted viral genes were detected. These pathogens were found in Gabonese rodents, mainly Lophuromys sp., Lemniscomys striatus and Praomys sp. We also identified new genotypes: Candidatus Bartonella gabonensis and Uncultured Anaplasma spp. This study shows that rodents in Gabon harbor some human pathogenic bacteria and protozoans. It is necessary to determine whether the identified microorganisms are capable of undergoing zoonotic transmission from rodents to humans and if they may be responsible for human cases of febrile disease of unknown etiology in Gabon.


Assuntos
Bactérias , Reservatórios de Doenças , Kinetoplastida , Roedores , Musaranhos , Toxoplasma , Vírus , Zoonoses , Animais , Bactérias/classificação , Bactérias/isolamento & purificação , Cidades , Gabão/epidemiologia , Humanos , Kinetoplastida/classificação , Kinetoplastida/isolamento & purificação , Roedores/microbiologia , Roedores/parasitologia , Roedores/virologia , Musaranhos/microbiologia , Musaranhos/parasitologia , Musaranhos/virologia , Toxoplasma/classificação , Toxoplasma/isolamento & purificação , Vírus/classificação , Vírus/isolamento & purificação , Zoonoses/microbiologia , Zoonoses/parasitologia , Zoonoses/virologia
20.
Vet Q ; 41(1): 137-151, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33618618

RESUMO

Brucellosis not only represents an important health restraint on livestock but also causes high economic losses in many developing countries worldwide. Despite considerable efforts made for the control of brucellosis, the disease is still spreading in many regions (such as the Middle East) where it represents one of the most important health hazards impacting both animals and humans. The present review aims to investigate the efficacy of veterinary control programs regarding brucellosis, with a special focus on current prevention, control, and eradication approaches. The reasons for unsuccessful control programs such as the absence of highly effective vaccines and non-certified bulls are also debated, to understand why the prevalence of brucellosis in livestock is not decreasing in many areas despite considerable efforts taken to date. The importance of governmental and regional investment in brucellosis control remains one of the main limiting factors owing to the limited budget allocated to tackle this disease. In this context, one health concept has generated novel comprehensive approaches with multiple economic implications across the livestock industry and public health. However, the implementation of such global preventive strategies appears to be a key issue for many endemic and low-income countries. According to the collected data, epidemiological contexts including management and trade systems along with well-defined agro-ecological zones should be evaluated in brucellosis endemic countries to improve milk production and to enhance the sustainability of the livestock sector at both national and regional levels.


Assuntos
Brucelose/veterinária , Saúde Única , Zoonoses/prevenção & controle , Animais , Brucella/isolamento & purificação , Brucella/patogenicidade , Brucelose/economia , Brucelose/imunologia , Brucelose/prevenção & controle , Microbiologia de Alimentos , Humanos , Gado , Leite/microbiologia , Vacinação/veterinária , Zoonoses/microbiologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...