Soyabean glycinin depresses intestinal growth and function in juvenile Jian carp (Cyprinus carpio var Jian): protective effects of glutamine.

This study investigated the effects of glycinin on the growth, intestinal oxidative status, tight junction components, cytokines and apoptosis signalling factors of fish. The results showed that an 80 g/kg diet of glycinin exposure for 42 d caused poor growth performance and depressed intestinal growth and function of juvenile Jian carp (Cyprinus carpio var. Jian). Meanwhile, dietary glycinin exposure induced increases in lipid peroxidation and protein oxidation; it caused reductions in superoxide dismutase (SOD), catalase and glutathione peroxidase (GPx) activities; and it increased MnSOD, CuZnSOD, GPx1b and GPx4a mRNA levels, suggesting an adaptive mechanism against stress in the intestines of fish. However, dietary glycinin exposure decreased both the activity and mRNA levels of nine isoforms of glutathione-S-transferase (GST) (α, µ, π, ρ, θ, κ, mGST1, mGST2 and mGST3), indicating toxicity to this enzyme activity and corresponding isoform gene expressions. In addition, glycinin exposure caused partial disruption of intestinal cell-cell tight junction components, disturbances of cytokines and induced apoptosis signalling in the distal intestines>mid intestines>proximal intestines of fish. Glycinin exposure also disturbed the mRNA levels of intestinal-related signalling factors Nrf2, Keap1a, Keap1b, eleven isoforms of protein kinase C and target of rapamycin/4E-BP. Interestingly, glutamine was observed to partially block those negative influences. In conclusion, this study indicates that dietary glycinin exposure causes intestinal oxidative damage and disruption of intestinal physical barriers and functions and reduces fish growth, but glutamine can reverse those negative effects in fish. This study provides some information on the mechanism of glycinin-induced negative effects.

Cardiac protein changes in rats after soybean oil treatment: a proteomic study.

BACKGROUND: Several studies show that the consumption of vegetable oils, such as soybean oil, rich in polyunsaturated fatty acids (PUFAs) has beneficial health effects by preventing or reducing the risk factors of cardiovascular diseases. While the demonstration of beneficial effects of the consumption of unsaturated fatty acids on the cardiovascular system has been proven in a macroscopic level, the molecular/cellular mechanisms responsible for this phenomenon are poorly understood. METHODS: In this work, a comparative proteomic approach, two-dimensional gel electrophoresis (2-DE) coupled to mass spectrometry (MALDI-TOF/TOF), was applied to investigate proteome differences in the left ventricle (LV) of rats that received 0.1 mL of soybean oil intramuscularly for 15 days (treated group-TR) and rats that had not (control group-CT). RESULTS: Soybean oil treatment improved left ventricular function, TR animals presented lower value of LVEDP and significantly changed LV proteome. The protein profile of VE revealed differences in the expression of 60 protein spots (p<0.05) between the experimental groups (CT and TR), 14 of those were identified by MS and MS/MS, and 12 of the 14 being non-redundant proteins. Robust changes were detected in proteins involved in cellular structure and antioxidant system and muscular contraction. CONCLUSIONS: The TR group presented an increase in the intensity of proteins involved in muscle contraction (myosin light chain-3 (3-MCL), creatine kinase M (CKM)) and thireodoxin, an antioxidant enzyme. Low intensity cytoskeletal protein, desmin, was also detected in TR animals. The results suggest that soybean oil induces changes in the levels of heart proteins which may partially account for the underlying mechanisms involved in the benefits provided by oils rich in polyunsaturated fatty acids.

Effects of dietary soybean oil on pig growth performance, retention of protein, lipids, and energy, and the net energy of corn in diets fed to growing or finishing pigs.

The objectives of this experiment were 1) to determine if dietary soybean oil (SBO) affects the NE of corn when fed to growing or finishing pigs, 2) to determine if possible effects of dietary SBO on the NE of corn differ between growing and finishing pigs, and 3) to determine effects of SBO on pig growth performance and retention of energy, protein, and lipids. Forty-eight growing (initial BW: 27.3 ± 2.5 kg) and 48 finishing (initial BW: 86.0 ± 3.0 kg) barrows were used, and within each stage of growth, pigs were allotted to 1 of 6 groups. Two groups at each stage of growth served as an initial slaughter group. The remaining 4 groups were randomly assigned to 4 dietary treatments and pigs in these groups were harvested at the conclusion of the experiment. A low-lipid basal diet containing corn, soybean meal, and no added SBO and a high-lipid basal diet containing corn, soybean meal, and 8% SBO were formulated at each stage of growth. Two additional diets at each stage of growth were formulated by mixing 25% corn and 75% of the low-lipid basal diet or 25% corn and 75% of the high-lipid basal diet. Results indicated that addition of SBO had no effects on growth performance, carcass composition, or retention of energy, protein, and lipids but increased (P < 0.05) apparent total tract digestibility of acid hydrolyzed ether extract and GE. Addition of SBO also increased (P < 0.05) DE and NE of diets, but had no effect on the DE and NE of corn. Finishing pigs had greater (P < 0.05) growth performance and retention of energy, protein, and lipids than growing pigs. A greater (P < 0.05) DE and NE of diets was observed for finishing pigs than for growing pigs and the DE and NE of corn was also greater (P < 0.05) for finishing pigs than for growing pigs. In conclusion, addition of SBO increases the DE and NE of diets but has no impact on the DE and NE of corn. Diets fed to finishing pigs have greater DE and NE values than diets fed to growing pigs and the DE and NE of corn are greater for finishing pigs than for growing pigs.

Fontes proteicas em suplementos para novilhos no período de transição seca-águas: características nutricionais / Protein sources in supplements for bulls in the dry-rainy transition season: nutritional characteristics

Avaliaram-se fontes proteicas em suplementos para novilhos em pastagens de Brachiaria decumbens Stapf., durante a transição seca-águas, sobre as características nutricionais. Utilizaram-se quatro animais Holandês x Zebu, não castrados, com média de peso de 300kg, fistulados no esôfago, rúmen e abomaso, distribuídos em delineamento em quadrado latino 4x4. Avaliaram-se suplementos isoproteicos, balanceados para 38 por cento de proteína bruta (PB), sendo um tratamento controle mistura mineral (MM); e três suplementos à base de grãos de milho moído + ureia (MU); grãos de milho moído + farelo de soja (MFS) e farelo de algodão 38 por cento PB (FA), fornecidos na quantidade de 0,17 por cento do peso vivo (PV). O consumo de fibra em detergente neutro pelos animais do tratamento MU, de 12,1g/kg PV, foi maior em 19,9 por cento do que o observado para os dos tratamentos MM e MFS, 10,2g/kg PV, e semelhante ao observado para os do tratamento FA, 11,0g/kg PV. Foram observados valores mais altos de digestibilidade aparente total da matéria seca (MS) para os tratamentos MU e MFS em relação aos demais. Os valores de pH ruminal, produção de nitrogênio microbiano e eficiência de síntese microbiana não foram influenciados pelos tratamentos. A suplementação com MU proporciona maior consumo e digestibilidade aparente total da MS em relação ao tratamento MM.

This study was conducted to evaluate protein sources in supplements for bulls grazing Brachiaria decumbens Stap., in the dry-rainy transition season, regarding nutritional characteristics. Four Holstein x Zebu bulls (300kg of body weight - BW) fitted with esophageal, ruminal, and abomasal cannulas, allotted in a latin square experimental design 4x4. The treatments were protein supplements with 38 percent of crude protein (CP) based on grounded corn grain + urea (GCU); grounded corn grain + soybean meal (GCSB); and cotton seed meal (38 percent CP) (CSM), supplied at a level of 0.17 percent BW, and a mineral mix control treatment (MM). The animals fed diets of GCU treatment showed neutral detergent fiber (NDF) intake of 12.1g/kg of BW, 19.9 percent greater than the observed on animals fed diets of MM and GCSB, 10.2 g/kg of BW, and similar to the NDF intake found for animals fed CSM treatment, 11.0g/kg of BW. The dry matter (DM) total apparent digestibility (TAD) was higher for GCU and GCSB treatments. The ruminal pH, microbial nitrogen production (MNP), and microbial synthesis efficiency (MSE) were not influenced by the treatments. The supplementation with corn and urea provides greater DM intake and digestibility when compared to the mineral mix.

Utilidad de las proteínas de pollo y de soja en ratas nutridas o desnutridas con diarrea inducida por lactosa / Utility of chicken and soybean protein in well-nourished or undernourished rats with lactose-induced diarrhea

En el tratamiento nutricional de la diarrea infantil se han utilizado con éxito preparaciones líquidas que contienen pollo combinado con cereales y/o tubérculos, que se preparan en la institución de hospitalización o en la casa. En un estudio anterior, se comparó la efectividad de fórmulas con proteínas de pollo o aislado de soja en el tratamiento de la diarrea infantil. El objetivo de este trabajo fue estudiar en ratas con o sin diarrea, la utilización de las proteínas y la disponibilidad de nutrientes de dietas preparadas con pollo o aislado proteico de soja, para determinar si los resultados obtenidos en los niños eran comparables a los obtenidos en ratas. Dado que la diarrea y la desnutrición frecuentemente están presentes de manera simultánea, también se estudiaron grupos de animales con o sin diarrea previamente desnutridos. Los resultados mostraron que durante la diarrea las ratas desnutridas fueron igualmente eficientes que las nutridas en utilizar los nutrientes presentes en las dietas con pollo o soja, por lo que no experimentaron un deterioro nutricional mayor que las nutridas. Adicionalmente, la severidad de la diarrea fue similar en las ratas asignadas a las dietas con pollo o soja. Asimismo, las ratas con diarrea consumieron, crecieron, absorbieron y retuvieron menos de los macronutrientes dietarios que las ratas control. Sin embargo, este efecto de la diarrea fue similar en las ratas que consumieron proteína de soja o de pollo. Como estos resultados coinciden con los obtenidos previamente en niños con diarrea aguda, se concluye que la proteína de soja es tan efectiva como la de pollo en el manejo nutricional de la diarrea y que la diarrea producida con lactosa en ratas es un modelo apropiado para el estudio de las consecuencias nutricionales de la misma.

In the nutritional treatment of diarrhea, good results have been obtained using liquid formulas with chicken meat together with cereals and/or starchy vegetables, prepared at home or at the clinic. In a previous study, formulas prepared with chicken meat or isolated soybean protein were tested in the treatment of infantile diarrhea. In order to determine if the results obtained in children could be reproduced in rats, the objective of the present study was to evaluate the effectiveness of the same diets in the nutritional treatment of well-nourished as well as undernourished rats with lactose induced diarrhea. The results showed that all the rats with diarrhea absorbed and retained less of the dietary nutrients and consequently, grew less than their counterparts without diarrhea. However, the absorption and retention measured in the nourished as well as in the undernourished rats were similar, indicating that a short period of malnutrition previous to the diarrhea episode, did not affect rats' absorption capacity. In addition, similarly to the results previously obtained in children, the rats with lactose induced diarrhea utilised equally well the dietary nutrients present in the chicken as well as in the soybean protein based diets. These results confirmed the value of isolated soybean protein in the nutritional treatment of diarrhea and showed that lactose induced diarrhea in rats is an appropriate model to study the nutritional consequences of diarrhea.

Effects of different dietary protein sources on expression of genes related to protein metabolism in growing rats.

Protein metabolism is known to be affected by dietary proteins, but the fundamental mechanisms that underlie the changes in protein metabolism are unclear. The aim of the present study was to test the effects of feeding growing rats with balanced diets containing soya protein isolate, zein and casein as the sole protein source on the expression of genes related to protein metabolism responses in skeletal muscle. The results showed that feeding a zein protein diet to the growing rats induced changes in protein anabolic and catabolic metabolism in their gastrocnemius muscles when compared with those fed either the reference protein casein diet or the soya protein isolate diet. The zein protein diet increased not only the mRNA levels and phosphorylation of mammalian target of rapamycin (mTOR), but also the mRNA expression of muscle atrophy F-box (MAFbx)/atrogin-1 and muscle ring finger 1 (MuRF1), as well as the forkhead box-O (FoxO) transcription factors involved in the induction of the E3 ligases. The amino acid profile of proteins seems to control signalling pathways leading to changes in protein synthesis and proteolysis.

Consumo de nutrientes e parâmetros ruminais de vacas alimentadas com diferentes níveis de energia na dieta / Intake of nutrients and ruminal parameters of cows fed different energy levels in the diet

Avaliou-se a influência de níveis de concentrado na dieta de bovinos. Utilizaram-se quatro vacas fistuladas no rúmen, distribuídas em quadrado latino 4x4, sobre o consumo, pH ruminal e nitrogênio amoniacal (N-NH3). Foi utilizada a silagem de milho, como volumoso, e concentrado, à base de milho, sorgo, farelo de soja, casca de soja, ureia e gordura protegida. As dietas foram isoproteicas com 18,6 por cento de proteína bruta, utilizando valores crescentes de extrato etéreo (5,7; 6,6; 7,5 e 8,4 por cento) para os níveis de concentrado de 30; 40; 50 e 60 por cento. Os valores médios de pH ruminal foram 6,71; 6,64; 6,63; 6,69 e as concentrações médias de N-NH3 de 37,40; 39,62; 35,59 e 34,13mg/100mL de líquido ruminal. O tratamento com 7,5 por cento de extrato etéreo apresentou média de consumo de fibra detergente neutro mais alto que os demais tratamentos, com máximo consumo de matéria seca estimado em 10,47kg/dia. Dietas com gordura protegida não interferiram no ambiente ruminal, até níveis de 8,4 por cento de extrato etéreo, apresentando melhor eficiência com relação ao consumo de 55 por cento de concentrado.

The influence of concentrate levels in the diet of cattle on intake, rumen pH, and ammonia nitrogen (NH3-N) using four cows fistulated in the rumen and distributed in 4x4 Latin square was evaluated. Corn silage was used as roughage and concentrate was maize, sorghum, soybean hulls, soybean rules, urea, and protected fat. Diets were isonitrogenous with 18.6 percent crude protein, using increasing amounts of ether extract (5.7, 6.6, 7.5, and 8.4 percent) to the levels of concentrate, 30, 40, 50 and 60 percent. The average values of rumen pH were 6.71, 6.64, 6.63, and 6.69 and the average concentrations of NH3-N were 37.40, 39.62, 35.59, and 34.13mg/100mL of rumen fluid. Animals fed 7.5 percent ether extract diet had higher neutral detergent fiber intake than the animals of other treatments with maximum dry matter intake estimated in 10.47kg/day. Protected fat diet did not interfere in the environment of rumen up to levels of 8.4 percent of ether extract, providing better efficiency with respect to intake of 55 percent concentrate.

Consumo e digestibilidade de nutrientes em novilhos suplementados e terminados em pasto, na seca / Intake and digestibility of nutrients in steers supplemented and finished on pasture in the dry season

Avaliaram-se o consumo e a digestibilidade de nutrientes por novilhos Nelore suplementados com elevado teor de proteína na terminação, em pasto, durante a seca. Foram utilizados quatro novilhos com 30 meses e peso médio inicial de 440kg, distribuídos em quadrado latino 4x4 (quatro níveis de suplementação e quatro períodos de 16 dias). Foram usados quatro piquetes de um hectare de capim Panicum maximum cv. Tanzânia, e os animais receberam: 0 (grupo-controle); 1,0; 2,0; ou 4,0kg de suplemento/dia. Os suplementos, à exceção do controle, apresentaram níveis decrescentes de proteína bruta (58 a 32 por cento da matéria seca) e proporções de mistura mineral:ureia:farelos de 10:10:80, 5:5:90 e 2,5:2,5:95 para os respectivos tratamentos. O consumo e a digestibilidade foram determinados com o uso do óxido crômico e FDAi, o delineamento estatístico foi em quadrado latino, e os tratamentos foram avaliados pela análise de regressão. Houve efeito linear crescente sobre o consumo e sobre os coeficientes de digestibilidade da matéria seca, proteína bruta, extrato etéreo e fibra em detergente neutro, e sobre o consumo de nutrientes digestíveis totais. Não houve efeito sobre o consumo de pasto e o consumo e a digestibilidade dos carboidratos não fibrosos. Os suplementos avaliados em geral melhoraram o consumo e a digestibilidade e, consequentemente, o valor nutritivo da dieta de novilhos em terminação sob pastejo, no período da seca.

The effects of supplements with high protein content on intake and digestibility were evaluated in Nellore steers finished at pasture during the dry season. Four steers avering 30-month old and initial weight of 440kg were distributed in a 4x4 Latin square (four levels of supplementation and four periods of 16 days). Four paddocks of one hectare containing Panicum maximum cv. Tanzania grass were utilized and the animals received 1.0, 2.0, or 4.0kg of supplement/day, in addition to a control - mineral mixture. The supplements, except control, presented decreasing levels of crude protein (58 to 32 percent in dry matter) and the proportions of mineral mixture:urea:cereal meals were 10:10:80, 5:5:90, and 2.5:2.5:95 for the treatments. The intake and digestibility were determined with the use of chromium oxide and ADFi. The statistical design was Latin square and the treatments were evaluated by regression analysis. There was an increasing linear effect on the intakes and digestibility coefficients of dry matter, crude protein, ether extract, neutral detergent fiber, and intake of total digestible nutrients. There was no effect on the intake of pasture and intake and digestibility of non-fiber carbohydrates. It can be concluded that the evaluated supplements generally improve the intake and digestibility and consequently the nutritive value of the diets of finishing animals on the pasture during the dry season.

Desempenho de novilhos suplementados e terminados em pasto, na seca, e avaliação do pasto / Performance of steers supplemented and finished on pasture in the dry season and pasture evaluation

Avaliaram-se o ganho de peso e as características de carcaça de novilhos Nelore suplementados com elevado teor de proteína na terminação em pasto, durante a seca, e estimou-se a disponibilidade dos componentes da pastagem. O experimento foi implantado em pastagem de Brachiaria brizantha, cv. Marandu, em seis piquetes de nove hectares. Foram utilizados 48 novilhos Nelore com 30 meses de idade e 415±16 kg. Cada lote, alojado em um piquete, foi pesado no início e a cada 21 dias, durante 84 dias. A suplementação constou de: 0 (grupo-controle); 0,25; 0,5; 1,0; 2,0; ou 4,0 kg/animal/dia. Os suplementos, à exceção do controle, apresentaram porcentagens decrescentes de proteína bruta (111 a 32 por cento da matéria seca) e proporções de mistura mineral:ureia:farelos de 25:25:50; 15:15:70; 10:10:80; 5:5:90 e 2,5:2,5:95, para os respectivos tratamentos. O delineamento foi o inteiramente ao acaso e os tratamentos foram avaliados pela análise de regressão. A proporção de colmo e folha seca aumentou no decorrer dos períodos experimentais, caracterizando o déficit hídrico da estação seca. Houve efeito linear crescente sobre o peso vivo final, ganho médio diário, peso de carcaça, espessura de gordura subcutânea e rendimento de carcaça em função do consumo de suplemento. A conversão do suplemento em kg/kg de ganho de peso, de 10:1, é típica de suplementação energética, mostrando que os animais não responderam satisfatoriamente à suplementação proteica.

Daily gain and carcass traits of Nellore steers fed different levels of supplementation with high content of protein at finishing during the dry season and the availability of the components of the pasture were evaluated. The experiment was carried out on pasture of Brachiaria brizantha, cv. Marandu, in six paddocks of nine hectares. Forty-eight Nellore steers averaging 30-month old and 415+16kg were used. Each group was allocated in a paddock, being weighted in the beginning and every 21 days, during 84 days. The levels of supplements were: 0.25, 0.5, 1.0, 2.0, and 4.0kg/animal/day, in addition to a control (mineral mixture). The supplements, except the control, presented decreasing levels of crude protein (111 to 32 percent of the dry matter) and proportions of mineral mixture:urea:concentrate feeds of 25:25:50, 15:15:70, 10:10:80, 5:5:90, and 2.5:2.5:95, respectively. The statistical design was completely randomized and the treatments were evaluated by regression analysis. The proportion of stem and dead leaf increased in elapsing of the experimental periods characterizing water deficit in the dry season. There was increasing linear effect on the final live weight, average daily gain, carcass weight, fat thickness, and carcass yield as a function of supplement intake. The supplement conversion in kg/kg of weight gain, at 10:1, is typical of energetic supplementation, showing that the animals did not satisfactorily respond to protein supplementation, which would give better values of conversion.

Use of biodiesel-derived crude glycerol for producing eicosapentaenoic acid (EPA) by the fungus Pythium irregulare.

Crude glycerol is a major byproduct for the biodiesel industry. Producing value-added products through microbial fermentation on crude glycerol provides opportunities to utilize a large quantity of this byproduct. The objective of this study is to explore the potential of using crude glycerol for producing eicosapentaenoic acid (EPA, 20:5 n-3) by the fungus Pythium irregulare . When P. irregulare was grown in medium containing 30 g/L crude glycerol and 10 g/L yeast extract, EPA yield and productivity reached 90 mg/L and 14.9 mg/L x day, respectively. Adding pure vegetable oils (flaxseed oil and soybean oil) to the culture greatly enhanced the biomass and the EPA production. This enhancement was due to the oil absorption by the fungal cells and elongation of shorter chain fatty acids (e.g., linoleic acid and alpha-linolenic acid) into longer chain fatty acid (e.g., EPA). The major impurities contained in crude glycerol, soap and methanol, were inhibitory to fungal growth. Soap can be precipitated from the liquid medium through pH adjustment, whereas methanol can be evaporated from the medium during autoclaving. The glycerol-derived fungal biomass contained about 15% lipid, 36% protein, and 40% carbohydrate, with 9% ash. In addition to EPA, the fungal biomass was also rich in the essential amino acids lysine, arginine, and leucine, relative to many common feedstuffs. Elemental analysis by inductively coupled plasma showed that aluminum, calcium, copper, iron, magnesium, manganese, phosphorus, potassium, silicon, sodium, sulfur, and zinc were present in the biomass, whereas no heavy metals (such as mercury and lead) were detected. The results show that it is feasible to use crude glycerol for producing fungal biomass that can serve as EPA-fortified food or feed.

A milk diet partly containing soy protein does not change growth but regulates jejunal proteins in young goats.

Soy protein is known to alter intestinal function and structure. We determined in young goats whether a diet partly containing soy protein differently affects intestinal morphology and the jejunal and hepatic proteome as compared with a milk diet. Fourteen male 2-wk-old White German dairy goat kids were fed comparable diets based on whole cow's milk in which 35% of the crude protein was casein (milk protein group; MP) or soy protein supplemented by indispensable AA (SPAA) for 34 d (n = 7/group). Body weight gain and food efficiency were not different. Jejunal and hepatic tissue was collected to determine intestinal morphology by microscopy and protein repertoire by 2-dimensional gel electrophoresis and mass spectrometry. Jejunal crypt depth was reduced and villus height to crypt depth ratio was higher in SPAA than in milk protein. Out of 131 proteins identified, 32 proteins were found to be differently expressed in both groups. In SPAA, down-regulated jejunal proteins were involved in processes related to cytoskeleton generation, protein, lipid, and energy metabolism. Downregulated hepatic proteins were related to glycolysis and Krebs cycle. Thirteen proteins were upregulated in SPAA. Among these, 2 hepatic proteins were related to carbohydrate breakdown. The other 11 jejunal proteins were involved in cytoskeleton assembly, proteolysis, and carbohydrate breakdown. In addition, glutathione-S-transferase was found to be upregulated in the medial jejunum. In conclusion, a SPAA diet as compared with a milk diet was related to changes in jejunal morphology and jejunal proteins relevant for protein turnover, energy metabolism, and cytoskeleton assembly with no apparent impact on animal BW gain.

A reduced protein diet induces stearoyl-CoA desaturase protein expression in pig muscle but not in subcutaneous adipose tissue: relationship with intramuscular lipid formation.

A reduced protein diet (RPD) is known to increase the level of intramuscular lipid in pig meat with a smaller effect on the amount of subcutaneous adipose tissue. This might be due to tissue-specific activation of the expression of lipogenic enzymes by the RPD. The present study investigated the effect of a RPD, containing palm kernel oil, soyabean oil or palm oil on the activity and expression of one of the major lipogenic enzymes, stearoyl-CoA desaturase (SCD) and on the level of total lipids and the fatty acid composition of muscle and subcutaneous adipose tissue in pigs. The RPD significantly increased SCD protein expression and activity in muscle but not in subcutaneous adipose tissue. The level of MUFA and total fatty acids in muscle was also elevated when the RPD was fed, with only small changes in subcutaneous adipose tissue. A positive significant correlation between SCD protein expression and total fatty acids in muscle was found. The results suggest that an increase in intramuscular but not subcutaneous adipose tissue fatty acids under the influence of a RPD is related to tissue-specific activation of SCD expression. It is suggested that the SCD isoform spectra in pig subcutaneous adipose tissue and muscle might be different.

Selectively hydrogenated soybean oil with conjugated linoleic acid modifies body composition and plasma lipids in rats.

The present study examined effects of a selectively hydrogenated soybean oil (SHSO) containing about 21% CLA on body composition, adipose depots and organ weights, and plasma lipid profiles in rats. Male Sprague Dawley rats were fed for 6 weeks a purified diet containing 0%, 1%, 3%, and 5% of SHSO. Different levels of SHSO supplementation did not significantly affect growth performance, although there was a trend toward decreased body weight gain with increasing dietary SHSO levels. The weights of inguinal, epididymal, and retroperitoneal adipose depot, but not mesenteric, were significantly influenced by dietary SHSO supplementation (P < 0.05, P < 0.01 and P < 0.001, respectively). Although the absolute weight of body protein in the control rats was higher in SHSO-fed rats, the effect on absolute weight of body protein is diluted and eliminated when the data are adjusted for eviscerated carcass weight as a percentage base. Therefore, as dietary SHSO level increased, body protein as a percentage of carcass weight increased (P < 0.05), although as dietary SHSO level increased, body fat proportion in carcass decreased (P < 0.01). Plasma triglycerides (TG) and total cholesterol (TC) concentrations were beneficially decreased, and HDL-cholesterol (HDL-C) to TC ratio was also beneficially increased by SHSO supplementation (P < 0.05, P < 0.001, and P < 0.01, respectively). However, plasma HDL-C concentration undesirably decreased with dietary SHSO supplementation (P < 0.05). The present study observed that body composition and plasma lipids were beneficially modulated by SHSO supplementation at least 3% levels (0.6% of CLA), and suggested that SHSO is a useful fat source because of the high level of CLA.

Dietary protein-related changes in hepatic transcription correspond to modifications in hepatic protein expression in growing pigs.

In a previous investigation we showed by expression profiling based on transcription analysis using differential display RT-PCR (DDRT-PCR) and real-time RT-PCR that a soy protein diet (SPI) significantly changes the hepatic transcription pattern compared with a casein diet (CAS). The present study was conducted to determine whether the transcriptional modulation is translated into protein expression. The hepatic mRNA abundance of four genes (EP24.16, LC3, NPAP60L, RFC2) that showed diet-related expression in previous DDRT-PCR experiments was analyzed by real-time RT-PCR. Two pigs that showed the most prominent SPI-related changes of transcription and two casein-fed pigs were selected and their hepatic protein pattern was studied comparatively by two-dimensional gel electrophoresis and peptide mass fingerprinting. The two-dimensional protein gel electrophoresis revealed a predominant SPI-associated upregulation of protein expression that corresponded to the results of the mRNA study. Of 380 diet-related protein spots displayed, 215 appeared exclusively or enlarged in the two SPI pigs; 10 of 39 diet-related expressed protein spots extracted could be identified by peptide mass fingerprinting and database search. Compared with the transcriptomics approach, the proteomics approach led in part to the identification of the same diet-associated expressed molecules (plasminogen, trypsin, phospholipase A2, glutathione-S-transferase alpha, retinal binding protein) or at least molecules belonging to the same metabolic pathways (protein and amino acid metabolism, oxidative stress response, lipid metabolism). The present results at the proteome level confirm SPI-related increased oxidative stress response and significant effects on protein biosynthesis already observed at the transcriptome level.

Protein turnover, amino acid profile and amino acid flux in juvenile shrimp Litopenaeus vannamei: effects of dietary protein source.

The effect of dietary protein on protein synthesis and growth of juvenile shrimps Litopenaeus vannamei was investigated using three different diets with equivalent protein content. Protein synthesis was investigated by a flooding dose of tritiated phenylalanine. Survival, specific growth and protein synthesis rates were higher, and protein degradation was lower, in shrimps fed a fish/squid/shrimp meal diet, or a 50% laboratory diet/50% soybean meal variant diet, than in those fed a casein-based diet. The efficiency of retention of synthesized protein as growth was 94% for shrimps fed the fish meal diet, suggesting a very low protein turnover rate; by contrast, the retention of synthesized protein was only 80% for shrimps fed the casein diet. The amino acid profile of the casein diet was poorly correlated with that of the shrimps. 4 h after a single meal the protein synthesis rates increased following an increase in RNA activity. A model was developed for amino acid flux, suggesting that high growth rates involve a reduction in the turnover of proteins, while amino acid loss appears to be high.

Microbial phytase does not improve protein-amino acid utilization in soybean meal fed to young chickens.

Three growth trials were conducted with young chicks to evaluate crude protein (CP) utilization in soybean meal (SBM) as affected by dietary addition of microbial phytase. In assay 1, chicks were fed two CP-deficient (50 or 150 g CP/kg) levels of dehulled SBM, and each SBM level was then supplemented with equimolar amounts of cystine or methionine (Met) or with 1200 U phytase/kg. At 50 g CP/kg, cystine or Met supplementation improved (P < 0.05) measures of growth performance, but when 150 g CP/kg from SBM was fed, only Met addition improved (P < 0.05) weight gain, food efficiency and protein efficiency ratio (PER). Thus, Cys was more limiting than Met in the diet that contained 50 g CP/kg, but Met was clearly first-limiting in the diet that contained 150 g CP/kg. Phytase supplementation did not improve (P > 0.10) chick performance at either level of CP. Chicks in assay 2 were fed 100 g CP/kg furnished by SBM, casein or corn gluten meal in the absence and presence of 1200 U phytase/kg. Weight gain, gain/food and PER values were greater (P < 0.05) in chicks fed SBM than in those fed casein, and greater (P < 0.05) in chicks fed casein than in those fed corn gluten meal. Phytase supplementation had no effect (P > 0.10) on any measure of chick performance, regardless of the protein source fed. In assay 3, three deficient levels of CP (50, 100 and 150 g/kg) from SBM were fed in the absence and presence of 1200 U dietary phytase/kg. Weight gain, food efficiency and protein accretion increased linearly (P < 0.05) as a function of protein intake, but phytase supplementation had no effect (P > 0.10) on slopes of the body weight and protein accretion curves. Likewise, phytase addition did not affect (P > 0.10) measures of protein utilization, i.e., weight gain/protein intake and protein gain/protein intake at any of the CP levels that were fed. Because sulfur amino acids are the growth-limiting factors when protein-deficient levels of SBM are fed to young chicks, we conclude that dietary addition of phytase does not improve sulfur amino acid utilization in SBM.

Alergia a proteína do leite da vaca / Allergy to cow's milk protein of

Trata-se de estudo sobre aleitamento natural e artificial, onde foi pesquisado na literatura vantagens e desvantagens do aleitamento artificial, quando utilizado leite de vaca, leite de soja e leite de cabra, como alimento unico na dieta do lactente. Conclui-se que o leite de vaca, e o mais alergenico dos leites consumidos

Infant feeding: a critical look at infant formulas.

Commercially available infant formulas serve as the best alternative to human milk when breastfeeding is not possible. Infant formulas are designed specifically to mimic the composition of human milk or the functional aspects of human milk feeding. This review highlights the issues related to the composition of infant formulas. The most hotly debated issue currently is whether to add long-chain polyunsaturated fatty acids to infant formulas. Other controversial topics include the safety and efficacy of soy-based protein formulas, protein quantity and quality as they relate to the infant's nutritional needs and feeding tolerance, and the replacement of lactose with other carbohydrate sources for specialized infant formulas. Recent modifications in the fat blend of infant formulas have led to improved fat digestibility. However, the full spectrum of benefits associated with the addition of nucleotides awaits further study. Modifications to infant formulas are made when the preponderance of scientific evidence suggests that the compositional change will better meet the nutritional needs of the infant.

Two-week dietary soy supplementation has an estrogenic effect on normal premenopausal breast.

An association has been reported between consumption of a high soy diet and a low incidence of breast cancer within populations of Southeast Asia. Phytoestrogens present in soy act as partial estrogen agonists or antagonists and can inhibit breast cancer cell proliferation in vitro. The effect of 14-day dietary soy supplementation with 60 g (45 mg isoflavones) on the normal breast of 84 premenopausal patients was determined. Serum concentrations of the isoflavanoids, genistein, daidzein, and equol, were raised in patients after soy supplementation (P < or = 0.025). Nipple aspirate (NA) levels of genistein and daidzein were higher than paired serum levels, both before (P < 0.001 and P = 0.001, respectively) and after soy supplementation (P < 0.001 and P = 0.049, respectively); however, there was no significant increase in NA isoflavone levels in response to soy. NA levels of apolipoprotein D were significantly lowered and pS2 levels raised in response to soy supplementation (P < or = 0.002), indicative of an estrogenic stimulus. No effect of soy supplementation on breast epithelial cell proliferation, estrogen and progesterone receptor status, apoptosis, mitosis, or Bcl-2 expression was detected. In conclusion, short term dietary soy has a weak estrogenic response on the breast, as measured by nipple aspirate apolipoprotein D and pS2 expression. No antiestrogenic effect of soy on the breast was detected.

Utilización de nutritientes en niños con diarrea aguda alimentados con formula a base de pollo y de soya / Nutrient utilization in children with acute diarrhea fed formulas with chicken or soybean protein

El objetivo de este trabajo fue comparar dos fórmulas, una a base de proteína de pollo y ocumo elaborada en base a la utilizada en el Hospital de Niños J.M. de los Ríos y otra a base de aislado proteico de soya en niños con diarrea aguda. Se realizó un estudio metabólico de 48h con dos grupos de 14 niños varones con edad promedio de 9 meses. Los niños alimentados con pollo consumieron mas proteina y menos grasa, ya que esta fórmula contenía mas proteína y menos grasa. La excreción fecal fue similar para los dos grupos (800 g/48h), sin embargo los niños que consumieron mas proteína (pollo) y grasa (soya) excretaron también una mayor cantidad de estos macronutrientes en las heces (diferencias no significativas). La absorción de grasa, nitrógeno y carbohidratos fue similar en ambos grupos y se aproximaron a 63 por ciento, 75 por ciento, y 78 por ciento, respectivamente; así como la retención de nitrógeno que fue del 55 por ciento. No se encontraron diferencias significativas en relación a los porcentajes de absorción y retención de energía de las dos fórmulas. La excreción de amoníaco fue mayor y de la creatinina menor que la de los niños sanos pero similar para los dos grupos de niños. No se encontraron diferencias en la excreción de urea. Los porcentajes de retención de Zn y Ca fueron similares para las dos fórmulas (47 por ciento y 72 por ciento, respectivamente). Se pudo detectar en ambos grupos una excreción urinaria de vitamina A que fue en promedio de 0,17 mg/48h. Los resultados señalan que desde el punto de vista nutricional la fórmula de soya fue utilizada tan apropiadamente como la fórmula hospitalaria de pollo para la alimentación durante la diarrea