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1.
Braz. j. biol ; 84: e255664, 2024. graf
Article in English | LILACS-Express | MEDLINE, LILACSEXPRESS, LILACS, VETINDEX | ID: biblio-1360227

ABSTRACT

Abstract Magnolia biondii Pamp is an important ornamental tree species widely grown and used as a rootstock in the propagation of different Magnolia varieties. In the current studies, anatomical, physiological and endogenous hormones were studied to check the effect of IBA 750 mg/L on the adventitious rooting and to provide theoretical and technical support for the propagation of Magnolia biondii Pamp through stem cuttings. Two thousand stem cuttings were prepared and divided into two groups i.e., IBA treated cuttings and water control. For the evaluation of antioxidant enzyme activities, and endogenous hormones levels, samples were collected on the day of planting and each 5th day and further steps were carried out in the laboratory according to the protocols and proper precautions. For the anatomical observations, samples were collected on the 13th, 15th, and 17th day for IBA treated cuttings while 21st, 23rd, and 25th day for control. Collected samples were preserved in the FAA solution and further observations were carried out in the laboratory. Anatomical observations showed that it took 13 days for the differentiation of root primordia to the appearance of young adventitious roots in IBA treated cuttings, while it took 21 days to develop primordia in the control. Antioxidant enzyme activities involved in ROS were significantly higher in the IBA treated cuttings compared to control. POD showed a peak on the 13th day before the emergence of roots in IBA treated cuttings while it showed a peak on the 21st day in the control. PPO showed a peak on the 21st day in the IBA treated cuttings while it showed a peak on the 29th day in the control. SOD showed a peak on the 17th day in IBA treated cuttings, while it showed a peak on the 25th day in the control. Exogenous application of IBA enhanced the endogenous IAA and GA3 levels compared to CK, while it reduced the levels of ABA continuously at the time of rooting and then increased gradually. Inclusively, our study suggests that IBA 750 mg/L is efficient for the rooting of Magnolia biondii Pamp cuttings, as it enhanced the process of antioxidant enzyme activities, endogenous hormones levels and reduced the time of root formation which is evident from the anatomical observations.


Resumo Magnolia biondii Pamp é uma importante espécie de árvore ornamental muito cultivada e utilizada como porta-enxerto na propagação de diferentes variedades de Magnolia. Nos estudos atuais, hormônios anatômicos, fisiológicos e endógenos foram estudados para verificar o efeito do AIB na dose de 750 mg / L no enraizamento adventício e fornecer suporte teórico e técnico para a propagação de M. biondii Pamp por meio de estacas. Duas mil estacas foram preparadas e divididas em dois grupos, ou seja, tratadas com AIB e controle de água. Para a avaliação das atividades das enzimas antioxidantes e dos níveis de hormônios endógenos, as amostras foram coletadas no dia do plantio e a cada 5 dias, enquanto as demais etapas foram realizadas em laboratório de acordo com os protocolos e os devidos cuidados. Para as observações anatômicas, as amostras foram coletadas no 13º, 15º e 17º dias para estacas tratadas com AIB e no 21º, 23º e 25º dias para o controle. As amostras coletadas foram preservadas em solução FAA, e outras observações foram realizadas em laboratório. Observações anatômicas mostraram a necessidade de 13 dias para a diferenciação dos primórdios radiculares até o aparecimento de raízes adventícias jovens em estacas tratadas com AIB e de 21 dias para o desenvolvimento dos primórdios no controle. As atividades das enzimas antioxidantes envolvidas nas ROS foram significativamente maiores nas estacas tratadas com AIB em comparação com o controle. A POD apresentou pico no 13º dia antes da emergência das raízes nas estacas tratadas com AIB, enquanto no 21º dia apresentou pico no controle. A PPO teve pico no 21º dia nas estacas tratadas com AIB e no 29º dia no controle. A SOD apresentou pico no 17º dia nas estacas tratadas com AIB e no 25º dia no controle. A aplicação exógena de AIB aumentou os níveis endógenos de IAA e GA3 em relação ao controle, enquanto reduziu os níveis de ABA continuamente no momento do enraizamento e, em seguida, aumentou gradativamente. Inclusive, nosso estudo sugere que o AIB na dose de 750 mg / L é eficiente para o enraizamento de estacas de M. biondii Pamp, visto que potencializou o processo de atividades de enzimas antioxidantes e os níveis de hormônios endógenos, além de reduzir o tempo de formação de raízes, o que fica evidente nas observações anatômicas.

2.
Braz. j. biol ; 84: e254016, 2024. tab
Article in English | LILACS-Express | MEDLINE, LILACSEXPRESS, LILACS, VETINDEX | ID: biblio-1364529

ABSTRACT

Abstract The present study was conducted to isolate and characterize bacteria from water and soil sample taken from the Lahore Canal at different sites i.e. Mall Road, Mohlanwal and Khera site. Isolated bacterial strains were identified on the basis of morphological and biochemical tests. Identification was confirmed by culturing bacteria on selective media. Antibiotic resistance test was also performed to observe the resistance of bacteria against different antibiotics. Blood agar test was performed for identification of different pathogenic bacteria. The result revealed that water and soil samples of Lahore Canal Lahore from different sites were contaminated with Escherichia coli, Salmonella sp., Vibrio sp., Bacillus spp., Enterococcus sp. and Staphylococcus spp. Due to presence of these pathogens, this water is not suitable for any domestic and irrigation use. Study also revealed that water of the Lahore Canal is harmful for human health as it is contaminated with bacteria that can cause severe disease e.g., Escherichia coli can cause gastroenteritis, Bacillus spp. can cause nausea and vomiting, Enterococcus may infect urinary tract, Salmonella sp. is responsible for Bacteremia, Staphylococcus spp. can cause mild fever and Vibrio sp. can be the reason of cholera. Thus it is rendered unfit for any kind of human use even other than drinking like swimming, bathing, washing etc., until and unless some remedial measures are employed to eradicate pathogenic microorganisms by WASA and LWMS according to standards of WHO. Similarly, it is quite harmful, when and where ever it is used for irrigation without proper treatment.


Resumo O presente estudo foi realizado para isolar e caracterizar bactérias de amostras de água e solo retiradas do Canal Lahore, em Lahore, em diferentes locais, ou seja, Mall Road, Mohlanwal e Khera. As cepas bacterianas isoladas foram identificadas com base em testes morfológicos e bioquímicos. A identificação foi confirmada por cultura de bactérias em testes de meios seletivos. O teste de resistência aos antibióticos também foi realizado para observar a resistência das bactérias a diferentes antibióticos. Foi realizado o teste de ágar sangue para identificar diferentes bactérias patogênicas. O resultado revelou que amostras de água e solo do Canal Lahore, Lahore, de diferentes localidades estavam contaminadas com Escherichia coli, Salmonella sp., Vibrio sp., Bacillus spp., Enterococcus sp. e Staphylococcus spp. Por causa da presença desses patógenos, essa água não é adequada para qualquer uso doméstico e de irrigação. O estudo revelou que a água do Canal Lahore é prejudicial à saúde humana, pois está contaminada com bactérias que podem causar doenças graves, por exemplo: Escherichia coli pode ocasionar gastroenterite; Bacillus spp. pode causar náuseas e vômitos; Enterococcus sp. pode infectar o trato urinário; Salmonella sp. é responsável pela bacteremia; Staphylococcus spp. pode causar febre leve; e Vibrio sp. pode ser a razão da cólera. Assim, torna-se imprópria para uso humano, como natação, banho, lavagem etc., até que algumas medidas corretivas sejam empregadas para erradicar microrganismos patogênicos por WASA e LWMS de acordo com os padrões da OMS. Da mesma forma, é bastante prejudicial, quando usada para irrigação sem tratamento adequado.

3.
Braz. j. biol ; 84: e245592, 2024. tab, graf
Article in English | MEDLINE, LILACS, VETINDEX | ID: biblio-1355866

ABSTRACT

Abstract In recent years, the development of high-throughput technologies for obtaining sequence data leveraged the possibility of analysis of protein data in silico. However, when it comes to viral polyprotein interaction studies, there is a gap in the representation of those proteins, given their size and length. The prepare for studies using state-of-the-art techniques such as Machine Learning, a good representation of such proteins is a must. We present an alternative to this problem, implementing a fragmentation and modeling protocol to prepare those polyproteins in the form of peptide fragments. Such procedure is made by several scripts, implemented together on the workflow we call PolyPRep, a tool written in Python script and available in GitHub. This software is freely available only for noncommercial users.


Resumo Nos últimos anos, o desenvolvimento de tecnologias de alto rendimento para obtenção de dados sequenciais potencializou a possibilidade de análise de dados proteicos in silico. No entanto, quando se trata de estudos de interação de poliproteínas virais, existe uma lacuna na representação dessas proteínas, devido ao seu tamanho e comprimento. Para estudos utilizando técnicas de ponta como o Aprendizado de Máquina, uma boa representação dessas proteínas é imprescindível. Apresentamos uma alternativa para este problema, implementando um protocolo de fragmentação e modelagem para preparar essas poliproteínas na forma de fragmentos de peptídeos. Tal procedimento é feito por diversos scripts, implementados em conjunto no workflow que chamamos de PolyPRep, uma ferramenta escrita em script Python e disponível no GitHub. Este software está disponível gratuitamente apenas para usuários não comerciais.


Subject(s)
HIV Protease , Polyproteins , Software , Molecular Docking Simulation
4.
Braz. j. biol ; 83: e248746, 2023. graf
Article in English | MEDLINE, LILACS, VETINDEX | ID: biblio-1339351

ABSTRACT

Abstract Colorectal cancer (CRC) is one of the most common cancers leading to comorbidities and mortalities globally. The rational of current study was to evaluate the combined epigallocatechin gallate and quercetin as a potent antitumor agent as commentary agent for therapeutic protocol. The present study investigated the effect of epigallocatechin Gallate (EGCG) (150mg) and quercetin (200mg) at different proportions on proliferation and induction of apoptosis in human colon cancer cells (HCT-116). Cell growth, colonogenic, Annexin V in addition cell cycle were detected in response to phytomolecules. Data obtained showed that, the colony formation was inhibited significantly in CRC starting from the lowest concentration tested of 10 µg/mL resulting in no colonies as visualized by a phase-contrast microscope. Data showed a significant elevation in the annexin V at 100 µg/mL EGCG(25.85%) and 150 µg/mL quercetin (48.35%). Moreover, cell cycle analysis showed that this combination caused cell cycle arrest at the G1 phase at concentration of 100 µg/mL (72.7%) and 150 µg/mL (75.25%). The combined effect of epigallocatechin Gallate and quercetin exert antiproliferative activity against CRC, it is promising in alternative conventional chemotherapeutic agent.


Resumo O câncer colorretal (CCR) é um dos cânceres mais comuns, levando a comorbidades e mortalidade em todo o mundo. O racional do presente estudo foi avaliar a combinação de galato de epigalocatequina e quercetina como um agente antitumoral potente como agente de comentário para protocolo terapêutico. O presente estudo investigou o efeito de galato de epigalocatequina (EGCG) (150 mg) e quercetina (200 mg) em diferentes proporções na proliferação e indução de apoptose em células de câncer de cólon humano (HCT-116). O crescimento celular, colonogênico, anexina V, além do ciclo celular foram detectados em resposta a fitomoléculas. Os dados obtidos mostraram que a formação de colônias foi inibida significativamente no CRC a partir da concentração mais baixa testada de 10 µg/mL, resultando em nenhuma colônia conforme visualizado por um microscópio de contraste de fase. Os dados mostraram uma elevação significativa na anexina V a 100 µg/mL de EGCG (25,85%) e 150 µg/mL de quercetina (48,35%). Além disso, a análise do ciclo celular mostrou que essa combinação causou parada do ciclo celular na fase G1 na concentração de 100 µg/mL (72,7%) e 150 µg/mL (75,25%). O efeito combinado da epigalocatequina galato e quercetina exerce atividade antiproliferativa contra o CCR, é promissor como agente quimioterápico alternativo convencional.


Subject(s)
Humans , Colorectal Neoplasms/drug therapy , Catechin/analogs & derivatives , Catechin/pharmacology , Quercetin/pharmacology , Cell Cycle , Annexin A5 , Cell Line, Tumor , Cell Proliferation
5.
Braz. j. biol ; 83: e246651, 2023. tab, graf
Article in English | MEDLINE, LILACS, VETINDEX | ID: biblio-1285627

ABSTRACT

Abstract The medicinal attributes of honey appears to overshadow its importance as a functional food. Consequently, several literatures are rife with ancient uses of honey as complementary and alternative medicine, with relevance to modern day health care, supported by evidence-based clinical data, with little attention given to honey's nutritional functions. The moisture contents of honey extracted from University of Veterinary and Animal Sciences, Lahore honey bee farm was 12.19% while that of natural source was 9.03 ± 1.63%. Similarly, ash and protein contents of farmed honey recorded were 0.37% and 5.22%, respectively. Whereas ash and protein contents of natural honey were 1.70 ± 1.98% and 6.10 ± 0.79%. Likewise fat, dietary fiber and carbohydrates contents of farmed source documented were 0.14%, 1.99% and 62.26% respectively. Although fat, dietary fiber and carbohydrates contents of honey taken from natural resource were 0.54 ± 0.28%, 2.76 ± 1.07% and 55.32 ± 2.91% respectively. Glucose and fructose contents of honey taken out from honeybee farm were 27% and 34% but natural source were 22.50 ± 2.12% and 28.50 ± 3.54%. Glucose and fructose contents of honey taken out from honeybee farm were 27% and 34% but natural source were 22.50 ± 2.12% and 28.50 ± 3.54%. Similarly, sucrose and maltose contents of farmed honey were 2.5% and 12% while in natural honey were 1.35 ± 0.49% and 8.00 ± 1.41% respectively. The present study indicates that such as moisture, carbohydrates, sucrose and maltose contents were higher farmed honey as compared to the natural honey. In our recommendation natural honey is better than farmed honey.


Resumo Os atributos medicinais do mel parecem ofuscar sua importância como alimento funcional. Consequentemente, várias literaturas estão repletas de usos antigos do mel como medicina complementar e alternativa, com relevância para os cuidados de saúde modernos, apoiados por dados clínicos baseados em evidências, com pouca atenção dada às funções nutricionais do mel. O teor de umidade do mel extraído da Universidade de Veterinária e Ciências Animais, fazenda de abelhas de Lahore, foi de 12,19%, enquanto o de fonte natural foi de 9,03 ± 1,63%. Da mesma forma, os teores de cinzas e proteínas do mel cultivado foram de 0,37% e 5,22%, respectivamente. Já os teores de cinzas e proteínas do mel natural foram de 1,70 ± 1,98% e 6,10 ± 0,79%. Da mesma forma, os teores de gordura, fibra dietética e carboidratos de origem cultivada documentados foram de 0,14%, 1,99% e 62,26%, respectivamente. Embora os teores de gordura, fibra alimentar e carboidratos do mel retirado dos recursos naturais fossem de 0,54 ± 0,28%, 2,76 ± 1,07% e 55,32 ± 2,91%, respectivamente. Os conteúdos de glicose e frutose do mel retirado da fazenda de abelhas foram de 27% e 34%, mas a fonte natural foi de 22,50 ± 2,12% e 28,50 ± 3,54%. Os conteúdos de glicose e frutose do mel retirado da fazenda de abelhas foram de 27% e 34%, mas a fonte natural foi de 22,50 ± 2,12% e 28,50 ± 3,54%. Da mesma forma, os teores de sacarose e maltose no mel cultivado foram de 2,5% e 12%, enquanto no mel natural foram de 1,35 ± 0,49% e 8,00 ± 1,41%, respectivamente. O presente estudo indica que os teores de umidade, carboidratos, sacarose e maltose foram maiores no mel cultivado em comparação ao mel natural. Em nossa recomendação, o mel natural é melhor que o mel de cultivo.


Subject(s)
Animals , Honey , Bees , Carbohydrates
6.
J. Health Biol. Sci. (Online) ; 10(1): 1-8, 01/jan./2022. ilus
Article in Portuguese | LILACS | ID: biblio-1361739

ABSTRACT

Objetivos: contribuir para a compreensão do desenvolvimento histórico do conceito de ansiedade, das classificações dos transtornos ansiosos e suas manifestações clínicas, bem como para a atualização sobre o processo de avaliação diagnóstica. Métodos: todas as edições das classificações da Organização Mundial de Saúde (OMS) - CID e da Associação Psiquiátrica Americana (APA) - DSM foram examinadas, além de livros-texto de referência de psiquiatria clínica, de publicações sobre a história e a evolução desses diagnósticos e de publicações produzidas pelas equipes responsáveis pela revisão das classificações atuais. Resultados: entende-se por ansiedade um estado afetivo normal, como um sintoma ou um termo para nomear um grupo de transtornos mentais. Nas primeiras e subsequentes edições das classificações da OMS e da APA, entre 1948 e 1975, os quadros ansiosos faziam parte do grupo das psiconeurose/neuroses. A partir do DSM-III (1980), o grupo das neuroses foi fragmentado em diversos outros, entre os quais os transtornos de ansiedade, o que foi seguido pela CID-10 (1992), apesar de alguma distinção na composição dos subtipos. Para as últimas versões, houve um empenho de compatibilização entre as duas, contudo restaram diferenças: o DSM-5 (2013) adota critérios diagnósticos; a CID-11 (2019) utiliza descrições clínicas e diretrizes diagnósticas, além de abordagens dimensionais para alguns transtornos. Conclusão: ocorreram modificações nas classificações psiquiátricas atuais, no grupo dos transtornos de ansiedade, que precisam ser disseminadas e agregadas a estratégias de formação e qualificação profissionais, incrementando habilidades diagnósticas e permitindo uma comunicação mais uniforme e precisa na prática clínica.


Objectives: to contribute to the understanding of the historical development of the concept of anxiety, the classifications of anxiety disorders and their clinical manifestations, as well as to update on the diagnostic evaluation process. Methods: all editions of the classifications of the World Health Organization (WHO) - ICD and the American Psychiatric Association (APA) - DSM were examined, in addition to reference textbooks on clinical psychiatry, publications on the history and evolution of these diagnoses, and scientific articles produced by the teams responsible for reviewing the current classifications. Results: anxiety is understood as a normal affective state, as a symptom, or as a term to name a group of mental disorders. In the first and subsequent editions of the WHO and APA classifications, between 1948 and 1975, anxiety disorders were part of the psychoneuroses/neuroses group. As of DSM-III (1980) onwards, the neuroses group was fragmented into several others, including anxiety disorders, which was followed by ICD-10 (1992), despite some different choices of subtypes. For the latest versions, there was a compatibility effort between them. However, differences remained: DSM-5 (2013) adopts diagnostic criteria; ICD-11 (2019) uses clinical description and diagnostic guidelines, in addition to dimensional approaches for some disorders. Conclusion: modifications have occurred in current psychiatric classifications, in the group of anxiety disorders, which need to be disseminated and added to professional training and qualification strategies, increasing diagnostic skills and providing for more uniform and accurate communication in clinical practice.


Subject(s)
Anxiety , Anxiety Disorders , Psychiatry , Health , Health Strategies , Diagnosis , History , Mental Disorders
7.
Braz. j. oral sci ; 21: e225042, jan.-dez. 2022. ilus
Article in English | LILACS, BBO - Dentistry | ID: biblio-1354728

ABSTRACT

Aim: Although bulk fill composites have been widely used as restorative material, there is no consensus regarding the best clinical protocol in terms of composite technique and adhesive system. Therefore, this clinical trial evaluated the clinical performance of bulk fill composites for class I restorations under different protocols. Methods: A randomized clinical trial including 155 class I restorations was conducted using different adhesive systems: conventional technique (phosphoric acid + conventional three-step adhesive system) (Group 1, 2 and 3); or self-etching adhesive system (Groups 4, 5 and 6). Control groups 1 and 4 were restored with conventional composite; groups 2 and 5 with low viscosity bulk fill and conventional composite as occlusal coverage; groups 3 and 6 with high viscosity bulk fill. The FDI criteria was used for clinical evaluation at baseline and after 6 months. Results: All groups showed good clinical performance. At baseline, the adhesive system did not affect postoperative hypersensitivity. After 6 months, group 5 showed a significant reduction in color and translucency; group 6 a reduction in terms of anatomical form and for postoperative sensitivity and an improvement in patient satisfaction (p<0.05). Considering the same restorative technique, the use of the self-etching adhesive system showed a significant decrease in color and translucency (p<0.05). Conclusion: All groups showed favorable clinical performance, and promising results were found for the conventional adhesive system and high viscosity bulk fill protocol


Subject(s)
Phosphoric Acids , Adhesives , Composite Resins , Dental Restoration, Permanent , Esthetics, Dental , Clinical Studies as Topic
8.
Braz. j. oral sci ; 21: e225232, jan.-dez. 2022. ilus
Article in English | LILACS, BBO - Dentistry | ID: biblio-1354773

ABSTRACT

Aim: The objective of this study was to describe a case series concerning internal bleaching of anterior traumatized teeth that underwent regenerative endodontic procedures (REP). Methods: Seven non-vital maxillary anterior teeth discolored after regenerative endodontic procedures were included and divided into two groups according to the medication protocol used in the REP: (1) Triple antibiotic paste (TAP) group (n=4); (2) Calcium hydroxide and 2% chlorhexidine gel (HC+CHX) (n=3). The bleaching technique used was walking bleach, where sodium perborate associated with distilled water was used. Bleaching agent was replaced weekly until the darkened tooth was slightly lighter than the adjacent tooth. The color was recorded with the aid of a digital spectrophotometer in two moments (T1: prior the first session of bleaching, T2: fourteen days after the last session of bleaching). The change in color after the procedure (ΔE) was calculated and reported in a descriptive analysis. Results: The ΔE for all teeth showed color differences exceeding the perceptibility threshold (ΔE > 3.7). Both groups showed similar ΔE (TAP: 18.3 ± 11.5; HC+CHX: 14 ± 11.2) at the end of the treatment. The average number of sessions needed to achieve satisfactory results was 1.7 ± 0.6 for HC+CHX group and 2.3 ± 0.5 for TAP group. Conclusion: Internal bleaching with sodium perborate associated with distilled water is effective in treating discolored teeth after regenerative endodontic procedures


Subject(s)
Tooth Bleaching , Tooth Discoloration , Regenerative Endodontics
9.
Braz. j. oral sci ; 21: e225272, jan.-dez. 2022. tab
Article in English | LILACS, BBO - Dentistry | ID: biblio-1354777

ABSTRACT

Aim: To compare the pre-clinical and clinical students` perceptions about the non-pharmacological behaviour management techniques in paediatric dentistry and to investigate the influence of the dental curriculum on the students` knowledge regarding this issue. Methods: A total of 283 students from the IV-and X-semester completed a questionnaire, consisted of 12 statements, describing the nonpharmacological behaviour management techniques for the treatment of paediatric dental patients. The acceptability rate was evaluated with a Likert scale ranging from 1 to 5. Results: The students from all courses demonstrated high acceptance for Reinforcement and Desensitization techniques and low for the Negative reinforcement and Physical restraint. The comparison between the perceptions of the pre-clinical and clinical students demonstrated a statistically significant increase in the acceptance of the physical restraint, along with Nonverbal communication, Modelling and Parental presence/absence (p<0.05). Conclusion: The results provide information about the students' knowledge and skills in behaviour management techniques together with some insights about how the educational process can modify the students` perceptions and views in dealing with paediatric dental patients


Subject(s)
Humans , Male , Female , Students, Dental , Behavior , Surveys and Questionnaires , Pediatric Dentistry , Methods
10.
Braz. j. oral sci ; 21: e225580, jan.-dez. 2022. tab
Article in English | LILACS, BBO - Dentistry | ID: biblio-1354782

ABSTRACT

Aim: This study aimed to evaluate the decision-making by patients to replace temporary restorations with permanent restorations after endodontic treatment and to verify the associated factors and evaluate the quality/integrity of the temporary restorative material within one month. Methods: This is a cross-sectional study using non-probabilistic sampling which analyzed patients after one month of endodontic treatment. The self-administered questionnaire contained sociodemographic, treatment decision-making and endodontic treatment questions. The restoration present in the mouth was evaluated in the clinical oral examination. The Poisson Regression test was used to verify the prevalence ratio. Results: The prevalence failure to perform permanent restorations was 61.1% of patients, and 42.7% reported not having adhered. The reasons are lack of time and not knowing the importance of replacing the restoration with a definitive one. The glass ionomer temporary restorative frequency was higher among those who chose not to replace the temporary restoration with a permanent one (PR=5.19; 95%CI 2.10-12.33). In addition, there was an association between the quality of the restorative material and the type of material, and the best clinical quality of the restoration was statistically associated with glass ionomer and composite resin. Conclusions: The findings show the importance of guidance by the dental surgeon in helping patients decide to replace their temporary restoration


Subject(s)
Humans , Male , Female , Surveys and Questionnaires , Dental Restoration, Permanent , Dental Restoration, Temporary , Endodontics
11.
Braz. j. oral sci ; 21: e226415, jan.-dez. 2022. tab
Article in English | LILACS, BBO - Dentistry | ID: biblio-1354995

ABSTRACT

Aim: Facial orthopaedic treatments based on the stimulation or restrictions of craniofacial bone growth are more effective when carried out during the pubertal growth spurt. The aim of this cross-sectional study was to evaluate the reproducibility of two cervical vertebrae methods (CVM) with manual tracing and direct visual inspection. Methods: A sample of 60 lateral cephalometric radiographs (10 of each of the 6 CVM stages) was randomly selected from 171 records. 5 orthodontists classified these radiographs according to the skeletal maturation stage in 2002 and 2005, and the application of both methods was conducted by direct visual inspection and evaluation through manual tracing. Results: The average reliability of the two methods determination and the two forms of evaluation was substantial. The direct visual inspection evaluation showed the highest reliability and agreement interexaminer values for both methods, as well as the intraexaminers evaluation. Conclusion: The reproducibility of CVM method was substantial, indicating its clinical use to determine the skeletal maturity and the ideal moment for treatment execution


Subject(s)
Bone Development , Cervical Vertebrae , Reproducibility of Results
12.
Braz. j. oral sci ; 21: e226252, jan.-dez. 2022. ilus
Article in English | LILACS, BBO - Dentistry | ID: biblio-1366526

ABSTRACT

Universal health coverage is a global target included in the United Nations Sustainable Development Goals agenda for 2030. Healthcare in Brazil has universal coverage through the Unified Health System (SUS), which guarantees health as basic right to the Brazilian population. Considering the principles of SUS, public oral healthcare management is a huge challenge. Aim: To identify good management practices for quality care adopted by local public oral healthcare managers and teams around Brazil. Methods: This study was registered with PROSPERO (CRD42017051639). Five databases (PubMed, Embase, Web of Science, Scopus and Lilacs) as well as the reference lists and citations of the included publications were searched according to PRISMA guidelines. Results:A total of 30,895 references were initially found, which were evaluated according to the defined eligibility criteria. Twenty qualitative studies, eight surveys and two mixed-model studies were selected. The practices (codes) were organized into three main groups (families), and the Frequency of the Effect Size (FES) of each code was calculated. Among the 20 codes identified, the most relevant ones were: Diagnosis and Health Planning (FES=80%) and Family Health Strategy(FES=66,7). The Intensity of the Effect Size of each study was also calculated to demonstrate the individual contribution of each study to the conclusions. Conclusion: The evidence emerging from this review showed that healthcare diagnosis, planning, and performance based on the family health strategy principles were the most relevant practices adopted by public oral healthcare managers in Brazil. The widespread adoption of these practices could lead to improved oral healthcare provision and management in Brazil


Subject(s)
Humans , Unified Health System , Dental Care , Health Management , Health Policy , Brazil
13.
Rev. enferm. UERJ ; 30: e63462, jan. -dez. 2022.
Article in English, Portuguese | LILACS-Express | LILACS | ID: biblio-1361558

ABSTRACT

Objetivo: analisar a associação entre a adesão de enfermeiros a um protocolo de prevenção de quedas e as variáveis demográficas, profissionais e de autoeficácia. Método: estudo transversal, com 23 enfermeiros de um hospital universitário de Cuiabá. Dados coletados por meio de questionário e da Escala de Autoeficácia Geral e Percebida. A medida da adesão dos enfermeiros ao protocolo de prevenção de quedas foi realizada pela verificação do número de registros de avaliação do risco de queda nos prontuários pelos enfermeiros nas 24 horas após admissão dos pacientes. Resultados: a idade média dos participantes foi de 37,56 anos e 91,3% eram do sexo feminino, com média de 11,43 anos de formação. Houve adesão de 39,13% ao protocolo e diferença significativa na adesão dos enfermeiros quando consideradas as clínicas (p=0,006). Conclusão: evidenciou-se associação entre a adesão de enfermeiros a um protocolo de prevenção de quedas e as unidades as quais eles trabalham. As características dos pacientes internados nessas unidades podem ter influenciado a adesão.


Objective: to analyze the association between nurses' adherence to a fall prevention protocol and demographic, professional and self-efficacy variables. Method: In this cross-sectional study, data were collected from 23 nurses at a university hospital in Cuiabá, using a self-efficacy questionnaire and the Perceived and General Self-Efficacy Scale. The nurses' adherence to the fall prevention protocol was measured by checking the number of fall risk assessment records in the nurses' records within 24 hours of patient admission. Results: participant mean age was 37.56 years and 91.3% were female, with an average of 11.43 years of training; 39.13% of the nurses adhered to the protocol and nurses' adherence differed significantly between units (p = 0.006). Conclusion: an association was found between nurses' adherence to a fall prevention protocol and the units where they worked. The characteristics of patients admitted to these units may have influenced adherence.


Objetivo: analizar la asociación entre la adhesión de enfermeros a un protocolo de prevención de caídas y las variables demográficas, profesionales y de autoeficacia. Método: estudio transversal junto a 23 enfermeros de un hospital universitario de Cuiabá. Los datos fueron recolectados a través de un cuestionario y de la Escala de Autoeficacia General. La medida de la adherencia de los enfermeros al protocolo de prevención de caídas se realizó mediante la verificación del número de registros de evaluación del riesgo de caídas en las historias de los enfermeros dentro de las 24 horas posteriores al ingreso del paciente. Resultados: la edad media de los participantes fue de 37,56 años y el 91,3% eran mujeres, con un promedio de 11,43 años de formación. La adhesión al protocolo fue del 39,13%. Hubo una diferencia significativa en la adherencia de los enfermeros en cuanto a las clínicas (p = 0,006). Conclusión: se evidenció la asociación entre la adherencia de los enfermeros a un protocolo de prevención de caídas y las unidades en las que laboran. Las características de los pacientes ingresados en estas unidades pueden haber influido en la adherencia.

14.
Rev. Odontol. Araçatuba (Online) ; 43(2): 49-54, maio-ago. 2022.
Article in Portuguese | LILACS, BBO - Dentistry | ID: biblio-1362035

ABSTRACT

Overdenture é uma prótese total que se caracteriza por usar apoios retentivos para que aumente sua estabilidade e retenção na cavidade bucal, gerando assim menor desconforto ao paciente e melhor adaptação. A Prótese overdenture dentossuportada necessita de elementos dentais remanescentes com canais tratados endodonticamente e que possuam suporte periodontal adequado. O objetivo deste relato de caso clínico é expor a sequência de confecção de uma prótese overdenture dentossuportada maxilar associada a uma prótese total convencional mandibular, com os passos clínicos detalhados por meio de uma abordagem teórica e visual. Esse tipo de reabilitação favorece a adaptação do paciente à nova condição, visto que o uso de próteses totais convencionais pode causar certo desconforto ao paciente devido à falta de retenção e estabilidade. Essa manutenção dos dentes remanescentes favorece à manutenção óssea e a futura decisão do paciente em optar pela instalação de implantes dentários, podendo confeccionar próteses implantorretidas (overdentures) ou implantossuportadas (protocolo).O Paciente demonstrou-se satisfeito com o resultado estético e funcional após a instalação da prótese concretizando que o tratamento é viável(AU)


Overdenture is a denture that utilizing retentive supports for increase stability and retention in oral cavity. The tooth-supported overdenture require dental elements with root canal therapy and good periodontal support. The aim of this study is to report the confection of a maxillary tooth-supported overdenture associated with mandibular denture and your clinical steps. This rehabilitation promotes the patient's adaptation the new condition, since the use of conventional dentures may cause discomfort due to lack of retention and stability. The preservation of dental elements promotes bone maintenance and forthcoming decision of the patient in the installation of dental implants that can choose implantretained or implant-supported prosthesis. Patient was satisfied with aesthetic and functional result after installation of prosthesis(AU)


Subject(s)
Humans , Male , Denture Retention , Denture, Complete , Denture, Overlay , Dental Prosthesis Retention , Dental Prosthesis
15.
Rev. Odontol. Araçatuba (Online) ; 43(2): 35-42, maio-ago. 2022. ilus
Article in Portuguese | LILACS, BBO - Dentistry | ID: biblio-1362023

ABSTRACT

Introdução: As cerâmicas odontológicas são amplamente utilizadas na Odontologia, desfrutando de grande popularidade devido a sua capacidade de mimetizar as características ópticas do esmalte e da dentina. O constante desenvolvimento de pesquisas tem desencadeado o lançamento de novos sistemas cerâmicos com propriedades aprimoradas e melhores resultados clínicos em longo prazo. Objetivo: O relato de caso aqui apresentado descreve uma reabilitação da zona estética do sorriso com laminados cerâmicos por meio de uma abordagem minimamente invasiva. Relato de caso: Paciente do gênero feminino, compareceu à clínica odontológica relatando insatisfação com a aparência do seu sorriso. Durante avaliação foram observadas, na região anterossuperior, restaurações extensas em resina composta deficientes nos incisivos, com proporção, volume, forma e texturização insatisfatórias, que em conjunto comprometia a zona estética do sorriso. Diante disso, após fotografias extra e intraorais, o tratamento proposto foi a reabilitação com laminados cerâmicos nas unidades 1.2 ao 2.2. Assim, foi realizado o enceramento e o mock-up, os quais serviram de orientação para os preparos dentários, a seguir, foram moldados e as restaurações confeccionadas no sistema IPS e.max. Após prova de cor e adaptação, os preparos e a superfície interna dos laminados cerâmicos foram tratados e cimentados com cimento resino fotopolimerizável. Conclusão: Instituindo uma abordagem conservadora, o plano de tratamento realizado através da reabilitação com laminados cerâmicos nas unidades dentárias anterossuperiores, permitiu o restabelecimento da função e estética, com reprodução das características dos dentes naturais e promoção do equilíbrio, da jovialidade e da harmonia no sorriso(AU)


Introduction: Dental ceramics are widely used in dentistry, enjoying great popularity due to the ability to mimic both enamel and dentin optical characteristics. The constant research development has triggered the launch of new ceramic systems with improved properties and better long-term clinical results. Objective: The case report presented here describes the zone rehabilitation of the aesthetic smile with ceramic laminates through a minimally invasive approach. Case report: Female patient, attended the dental clinic reporting dissatisfaction with the appearance of her smile. During the evaluation, extensive restorations in composite resin were observed in the incisors in the anterosuperior region, with unsatisfactory proportion, amount, shape and texturing, which together compromised the aesthetic zone of the smile. Faced with this, after extra and intraoral photographs, the proposed treatment was the restoration with the ceramic laminates in units 1.2 to 2.2. Thus, waxing and mock-up were performed, which served as guidance for the dental preparations, then they were molded and restorations fabricated in the IPS e.max system. After shade and adaptation proof, the preparations and the inner ceramic laminates surface were treated and cemented with light-cured resin cement. Conclusion: Adopting a conservative approach, the treatment plan carried out through restoration with ceramic laminates on the upper anterior teeth allowed the recovering function and aesthetics, reproducing characteristics of natural teeth and promoting balance, joviality and harmony in the smile(AU)


Subject(s)
Humans , Female , Ceramics , Dental Veneers , Esthetics, Dental , Smiling , Composite Resins , Incisor
16.
Lima; Perú. Ministerio de Salud. Dirección General de Intervenciones Estratégicas en Salud Pública. Dirección de Prevención y Control de Tuberculosis; 2 ed; Jun. 2022. 36 p. ilus.
Monography in Spanish | MINSAPERÚ, LILACS, LIPECS, MINSAPERÚ | ID: biblio-1371429

ABSTRACT

En el presente rotafolio encontrará conceptos básicos sobre la tuberculosis, sus síntomas, su forma de contagio y las medidas preventivas a realizar en un establecimiento de salud


Subject(s)
Signs and Symptoms , Tuberculosis , Tuberculosis, Pulmonary , Tuberculosis, Multidrug-Resistant
17.
Arch. argent. pediatr ; 120(3): 158-166, junio 2022. tab
Article in English, Spanish | LILACS, BINACIS | ID: biblio-1368135

ABSTRACT

Introducción. Existen estudios que dan cuenta del uso extendido de la medicina tradicional (MT) en diversos países. La formación del pediatra es escasa en este campo. Objetivo. Describir las características de la MT utilizada por los pacientes de un hospital pediátrico de la Ciudad Autónoma de Buenos Aires. Población y métodos. Diseño cualitativo, basado en la teoría fundada. Entrevista a cuidadores de niños de 0 a 11 años. Resultados. Se entrevistaron 30 personas de diferentes colectividades, la gran mayoría mujeres amas de casa. Casi la totalidad refirió haber utilizado la MT. La fuente de recomendación más utilizada fue la familia. En general, el uso de la MT precede a la consulta a un médico del sistema de salud. Se acude a la MT por entidades definidas por la medicina convencional, pero también por otras propias, como pata de cabra, ojeo y empacho. Las prácticas terapéuticas incluyen la administración de preparados a base de plantas, infusiones, caldos, como también rituales conducidos por curanderos. Todos los usuarios refirieron una valoración positiva de la MT. Una gran parte de los entrevistados consideró importante que el médico conozca y pregunte sobre ella. Todos refirieron que su uso no fue abordado en consultas previas. Conclusiones. La MT se extiende como conocimiento y/o práctica en toda la población estudiada y forma parte de su vida cotidiana. Los métodos utilizados son muy diversos, así como las situaciones para las que se utiliza; las principales causas son de origen gastrointestinal y respiratoria. No es abordada en la consulta pediátrica, pero sus usuarios desearían que lo fuera.


Introduction. Several studies have reported on the widespread use of traditional medicine (TM) in different countries. Pediatricians receive scarce training in this field. Objective. To describe the characteristics of TM used by patients attending a children's hospital in the City of Buenos Aires. Population and methods. Qualitative design based on grounded theory. Interview with caregivers of children aged 0-11 years. Results. Thirty people from different communities were interviewed, mostly homemakers. Almost all referred having used TM. The most common source of recommendation was the family. In general, TM use precedes the visit to a physician in the health system. TM is used to manage conditions defined by conventional medicine, but also to treat folk illnesses such as Simeon's disease, evil eye, and indigestion. Therapeutic practices include plant-based preparations, infusions, broths, as well as other rituals performed by folk healers. All users had a positive opinion about TM. Most interviewees considered that physicians should know and ask about TM. All participants referred that its use had not been addressed in prior visits. Conclusions. TM spreads as knowledge and/ or practice across the studied population and is part of everyday life. TM methods vary greatly, as well as the conditions for which it is used; the main reasons for use included gastrointestinal and respiratory conditions. It is not addressed in pediatric visits, but users wish it was.


Subject(s)
Humans , Physicians , Medicine, Traditional , Epidemiology, Descriptive , Pediatricians , Hospitals, Pediatric
18.
Interdisciplinaria ; 39(1): 41-56, jun. 2022. tab
Article in Spanish | LILACS-Express | LILACS | ID: biblio-1360479

ABSTRACT

Resumen La violencia en las parejas adolescentes es un tema de especial importancia por la implicación en la salud y en las relaciones afectivas de los adolescentes. El propósito de este estudio fue determinar la influencia del género y de la religión en la violencia física, de comunicación/relación y psicológica, recibidas por los adolescentes, y en la satisfacción con la vida que estos presentan, además, del papel que juegan las conductas sexistas en esta violencia. Los participantes han sido 1 036 adolescentes pertenecientes a siete centros de educación secundaria del sudeste español, de edades comprendidas entre 14 y 19 años, de los que el 48.1 % eran hombres y el 51.9 %, mujeres. Para alcanzar los objetivos de la investigación, junto con los datos demográficos y personales, se aplicó la Escala de Satisfacción con la Vida (SWLS), la Escala de Violencia en la pareja desarrollada y validada para este estudio que recoge tres subescalas: violencia psicológica, violencia física, y violencia en la comunicación y relación con otros. Finalmente, el cuestionario sobre conductas sexistas da información sobre creencias falsas y conductas sexistas. Los resultados muestran niveles más altos de violencia física, psicológica y de comunicación/relación sufrida por los hombres. Aunque son los hombres los que se sienten más satisfechos con la vida y los que manifiestan más conductas sexistas. La creencia religiosa no interviene en la violencia ejercida, pero son los adolescentes practicantes los que se muestran más satisfechos con la vida.


Abstract Teen dating violence is defined as the physical, sexual, psycho-emotional violence that occurs within couple relationships during adolescence. These are relationships that threaten the well-being, health or integrity of the couple and that use mechanisms of control or domination of the couple through coactive or coercive tactics. With this research we have tried to find out about life satisfaction, the physical, psychological and communicative violence suffered by adolescent men and women who are religious or not. In addition, detect sexist behaviours in young people of both sexes and assess behaviours based on religious practice. A descriptive, cross-sectional and predictive study was carried out. Cluster random sampling was carried out when selecting secondary schools in South-eastern Spain. The study included 1 036 adolescents with ages between 14 and 19 years old, of whom 48.1 % were men and 51.9 % women. To fulfill the objectives together with demographic and personal data, the Satisfaction with Life Scale (SWLS) is applied, which assesses the cognitive aspects of well-being. The Psychological Violence Scale is applied with the objective of assessing the psychological and emotional violence. To know the physical violence and authoritarian behaviour, the Physical Violence Scale is applied. Finally, the Violence in Communication and Relationship with Others Scale is introduced for studying online violence and relationship violence. The three violence scales have been adapted, developed and validated by Soriano and Aguilera (2017). Finally, the questionnaire on sexist behaviours collects information on false beliefs and sexist behaviours. The data collection procedure followed the standards set out in the Helsinki Convention (2013) for scientific research involving human participants. Regarding the results, in the first of the logistic regression models, it is good for boys to go out with many girls, but not the other way around (Wald = 14.461; p = .000; OR = 4.047), it is observed to be that boys are 4.047 times more likely to agree with this attitude than girls. Also, it is statistically significant that sometimes you have to threaten others to know who is in charge, (Wald = 8.107; p = .004; OR = 2.173), which indicates that the boys are 2.173 times more likely in accordance with that conduct that the girls. It is also statistically significant that when a woman is assaulted by her partner she will have done something to provoke him, (Wald = 16.315; p = .000; OR = 3.538), making it 3.538 times more likely that boys support this attitude than girls. At the same time, it is statistically significant that the violence that occurs within the home is a family matter and should not leave there, (Wald = 4.132; p = .042; OR = 1.694), which also indicates that boys are 1.694 times more likely to adopt this behaviour than girls. Regarding the perception of violence, it is observed that men perceive more violence than women, being significant for the three types of violence studied (p < .05): Communication, psychological and physical. Personal satisfaction also shows significant results (Z = -2.61; p = .009). In this case, men perceive greatersatisfaction with their life than women. For students with religious beliefs and practices, statistically significant results were evident only in personal satisfaction (Z = -3.42; p = .001), showing that students who practice a religion have more satisfaction with life. The results show higher levels of physical, psychological and communication / relationship violence suffered by men. However, men feel more satisfation with life and show more sexist behaviour. Religious belief does not intervene in violence, but the practicing adolescents are those most satisfied with life.

19.
São Paulo; s.n; 20220601. 80 p.
Thesis in Portuguese | LILACS, BBO - Dentistry | ID: biblio-1370736

ABSTRACT

Introdução e objetivo: A tecnologia digital tem desempenhado um papel cada vez mais importante na Odontologia há vários anos. Nos últimos anos, com a introdução da tomografia computadorizada (TC), da impressão tridimensional (3D), dos desenhos auxiliados por computador (CAD) e dos modelos feitas através destes desenhos (CAM), trouxeram uma grande evolução para os conceitos de tratamento na área da implantodontia. O objetivo deste estudo foi avaliar a acurácia de guias do tipo fresado e impresso a partir de um único planejamento virtual. Materiais e métodos: Foram selecionadas como amostras, dez manequins pré-fabricados com dentes ausentes com a finalidade de colocação de implantes. Estes manequins foram submetidos a tomografias computadorizadas de feixe cônico e a escaneamentos digitais através de um escâner intra oral, estas imagens foram sobrepostas em um software especifico de planejamentos digitais e assim gerados dois tipos de guias através de um mesmo planejamento digital. Guias impressos (grupo1) e guias fresados (grupo 2) foram confeccionados através de uma impressora tridimensional e uma fresadora respectivamente. Em cada manequim foram colocados dois implantes em regiões de dente 24 e dente 25, cada região com um guia diferente. Após a colocação dos implantes estes manequins foram submetidos a novas tomografias computadorizadas de feixe cônico onde foram adquiridas nova imagens, que foram sobrepostas com as imagens do planejamento inicial a fim de mensurar a fidelidade pós cirúrgica. Resultados: Foram avaliados desvios angulares e lineares, para os desvios angulares medianos, foi observada uma diferença significativa entre os grupos (p=0,033), com valores medianos de 3,78° (min-max: 1,32-8,75) para o guia impresso e 5,66° (3,06- 6,64) para o guia fresado, para desvios lineares do ápice, o resultado não demonstrou diferença significativa entre os grupos (p=0.423), com valores medianos de 0,73 (minmax: 0,53-1,82) para o guia impresso e 1,10 (0,26-2,47) para guia fresado. Conclusão: Houve diferenças significativas na acurácia da posição do implante apenas em relação ao desvio angular mediano, um dos fatores que pode ter contribuído é a extensão da extremidade livre que havia no guia cirúrgico fresado. Guias fresados utilizados em extremidade livre de dois dentes mostraram resultados semelhantes a extremidade menor (um dente), possibilitando a sua utilização dentro dos parâmetros clínicos aceitáveis e indicando futuros estudos de analises de tamanhos de extensão de extremidades.


Subject(s)
Surgery, Computer-Assisted
20.
São Paulo; s.n; 20220606. 73 p.
Thesis in Portuguese | LILACS, BBO - Dentistry | ID: biblio-1371265

ABSTRACT

O objetivo deste ensaio clínico randomizado foi comparar a taxa de sobrevida das restaurações oclusais e oclusoproximais em molares decíduos usando duas versões de cimentos de ionômero de vidro: pó-líquido, manipulado manualmente (MAN) e encapsulado (ENC) após 24 meses. Crianças entre 3 e 10 anos de idade que apresentaram lesões de cárie em dentina nas superfícies oclusais e/ou oclusoproximais de molares decíduos foram selecionadas na Faculdade de Odontologia da Universidade de São Paulo, Brasil. Elas foram alocadas aleatoriamente em dois grupos: M/M (Fuji IX®, GC Europa) ou ENC (Equia Fill®, GC Europa). A ocorrência de falha das restaurações foi avaliada por duas examinadoras calibradas e cegas e em relação aos grupos. As análises estatísticas foram realizadas no software Stata 13 (StataCorp, EUA). A análise de Kaplan-Meier foi utilizada para ilustrar a sobrevida e o comportamento das restaurações ao longo dos 24 meses de acompanhamento enquanto a Regressão de Cox com fragilidade compartilhada foi realizada para avaliar a associação entre falha de restauração e variáveis independentes (=5%). Realizamos ainda a análise por intenção de tratar (ITT) considerando os 24 meses de acompanhamento. Um total de 323 restaurações foram realizadas em 145 crianças. A sobrevida para o grupo MAN foi de 58,2% e 60,1% para o grupo ENC, sem diferença estatisticamente significativa (p=0,738). As restaurações oclusoproximais tiveram menor taxa de sobrevida quando comparadas às oclusais (HR=3,83; p<0,001). A taxa de sobrevida nos molares decíduos não é influenciada pelas diferentes formas de apresentação do CIV Registro do ECR: Este ensaio clínico randomizado foi registrado no ClinicalTrials.Gov em 15/10/2014 sob protocolo (NCT 02274142).


Subject(s)
Tooth, Deciduous , Pediatric Dentistry , Glass Ionomer Cements
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