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1.
Methods Mol Biol ; 2568: 147-163, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-36227567

RESUMEN

Small angle X-ray scattering (SAXS) has been widely applied as an enabling integrative technique for comprehensive analysis of the structure of biomacromolecules by multiple, complementary techniques in solution. SAXS in combination with computational modeling can be a powerful strategy bridging the secondary and 3D structural analysis of large RNAs, including the long noncoding RNAs (lncRNA). Here, we outline the major procedures and techniques in the combined use of SAXS and computational modeling for 3D structural characterization of a lncRNA, the subgenomic flaviviral RNA from Zika virus. lncRNA production and purification, RNA buffer and sample preparation for SAXS experiments, SAXS data collection and analysis, SAXS-aided RNA 3D structure prediction, and computational modeling are described.


Asunto(s)
ARN Largo no Codificante , Infección por el Virus Zika , Virus Zika , Simulación por Computador , Humanos , Modelos Moleculares , Conformación de Ácido Nucleico , Dispersión del Ángulo Pequeño , Difracción de Rayos X , Rayos X , Virus Zika/genética
2.
Braz. j. biol ; 83: e240118, 2023. tab, graf
Artículo en Inglés | LILACS, VETINDEX | ID: biblio-1278559

RESUMEN

Abstract For many centuries human populations have been suffering and trying to fight with disease-bearing mosquitoes. Emerging and reemerging diseases such as Dengue, Zika, and Chikungunya affect billions of people around the world and recently has been appealing to control with chemical pesticides. Malathion (MT) is one of the main pesticides used against mosquitoes, the vectors of these diseases. This study aimed to assess cytotoxicity and mutagenicity of the malathion for the bioindicator Allium cepa L. using a multivariate and integrative approach. Moreover, an appendix table was compiled with all available literature of insecticides assessed by the Allium cepa system to support our discussion. Exposures during 48h to 0.5 mg mL-1 and 1.0 mg mL-1 MT were compared to the negative control (distilled water) and positive control (MMS solution at 10 mg L-1). The presence of chromosomal aberrations, micronuclei frequency, and mitotic index abnormalities was evaluated. Anaphase bridges were the alterations with higher incidence and presented a significantly elevated rate in the concentration of 0.5 mg mL-1, including when compared to the positive control. The integrative discriminant analysis summarizes that MT in assessed concentrations presented effects like the positive control, corroborating its potential of toxicity to DNA. Therefore, it is concluded that MT in its pure composition and in realistic concentrations used, has genotoxic potential in the biological assessment of A. cepa cells. The multivariate integrative analysis was fundamental to show a whole response of all data, providing a global view of the effect of MT on DNA.


Resumo Por muitos séculos, as populações humanas sofrem e tentam combater os mosquitos transmissores de doenças. Doenças emergentes e reemergentes como Dengue, Zika e Chikungunya afetam bilhões de pessoas em todo o mundo e, recentemente, vem apelando ao controle com pesticidas químicos. O Malation (MT) é um dos principais pesticidas usados ​​contra mosquitos, vetores dessas doenças. O objetivo deste estudo foi avaliar a citotoxicidade e a mutagenicidade do MT para o bioindicador Allium cepa L. usando uma abordagem multivariada e integrativa. Além disso, uma tabela suplementar foi compilada com toda a literatura disponível de inseticidas avaliada pelo sistema Allium cepa para apoiar nossa discussão. Exposições ao MT durante 48h a 0,5 mg mL-1 e 1,0 mg mL-1 foram comparadas a um controle negativo (água destilada) e um controle positivo (10 mg L-1 de MMS). Foram avaliadas a presença de aberrações cromossômicas, frequência de micronúcleos e anormalidades no índice mitótico. As pontes anafásicas foram as alterações com maior incidência e apresentaram uma taxa significativamente elevada na concentração de 0,5 mg mL-1, inclusive quando comparadas ao controle positivo. A análise discriminante integrativa resume que o MT nas concentrações avaliadas apresentou efeitos semelhantes ao controle positivo, corroborando seu potencial de toxicidade para o DNA. Portanto, conclui-se que o MT, em sua composição pura e nas concentrações realistas utilizadas, possui potencial genotóxico na avaliação biológica de células de A. cepa. A análise integrativa multivariada foi fundamental para mostrar uma resposta completa de todos os dados, fornecendo uma visão global do efeito da MT no DNA.


Asunto(s)
Humanos , Animales , Virus Zika , Infección por el Virus Zika , Insecticidas/toxicidad , Daño del ADN , Aberraciones Cromosómicas , Raíces de Plantas , Cebollas , Mosquitos Vectores , Malatión/toxicidad , Índice Mitótico
3.
Methods Mol Biol ; 2575: 305-321, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-36301483

RESUMEN

Infectious agents often challenge therapeutics, from antibiotics resistance to antigenic variability affecting inoculation measures. Over the last decades, genome sequencing arose as an important ally to address such challenges. In bacterial infection, whole-genome-sequencing (WGS) supports tracking pathogenic alterations affecting the human microbiome. In viral infection, the analysis of the relevant sequence of nucleotides helps with determining historical variants of a virus and elucidates details about infection clusters and their distribution. Additionally, genome sequencing is now an important step in inoculation protocols, isolating target genes to design more robust immunisation assays. Ultimately, genetic engineering has empowered repurposing at scale, allowing long-lasting repeating clinical trials to be automated within a much shorter time-frame, by adjusting existing protocols. This is particularly important during sanitary emergencies as the ones caused by the 2014 West African Ebola outbreak, the Zika virus rapid spread in both South and North America in 2015, followed by Asia in 2016, and the pandemic caused by the SARS-CoV-2, which has infected more than 187 million people and caused more than 4 million deaths, worldwide, as per July 2021 statistics. In this scenery, this chapter presents a novel fully automated strategy to handle antigenic variability in immunisation protocols. The methodology comprises of two major steps (1) nanopore sequencing of infectious agent variants - the focus is on the SARS-CoV-2 and its variants; followed by (2) mRNA vector design for immunotherapy. This chapter presents the nanopore sequencing step and Chapter 17 introduces a protocol for mRNA vector design.


Asunto(s)
COVID-19 , Secuenciación de Nanoporos , Infección por el Virus Zika , Virus Zika , Humanos , SARS-CoV-2/genética , COVID-19/prevención & control , Inmunización , Variación Antigénica , ARN Mensajero , Virus Zika/genética
4.
Med Educ Online ; 28(1): 2139657, 2023 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-36331873

RESUMEN

INTRODUCTION: Over the past two years, coronavirus disease (COVID-19) has greatly altered medical student education as well as daily life. Medical schools across the world were disrupted and had to immediately adapt the educational experience to the online environment in order to continue the delivery of quality medical education. However, COVID-19 was not the only recent pandemic. This posed the question, were similar disruptions and adaptations also seen in recent past pandemics such as Severe Acute Respiratory Syndrome (SARS) or Middle East Respiratory Syndrome (MERS) that could have prepared medical educators for COVID-19? This scoping review investigated the educational and personal impact of recent pandemics on medical students. METHODS: This review followed the PRISMA-ScR guidelines for scoping reviews. Nine databases including PubMed, ERIC, and EMBASE were systematically searched using keywords and subject headings related to medical students and SARS, H1N1, MERS, Ebola, Zika, and COVID-19. Studies were limited to research studies published between 2000 and 2020 and in English. Based on exclusion and inclusion criteria, all studies were independently screened by two reviewers first by the title/abstract and then via full text. Data were extracted from the included studies and analyzed qualitatively using thematic analysis. RESULTS: A total of 174 studies fit the criteria. Seven major themes emerged from those studies: educational adaptations and online modifications, knowledge and attitudes of students, mental wellness of students, student involvement and use of telehealth, student vaccination, physical wellness of students, and stigma. CONCLUSION: This review provided insights into how medical students were affected by recent pandemics and their perceptions of pivoting to online education, mental health, and knowledge of the diseases. Additionally, this review showcases the various educational adaptations that emerged uniquely during the COVID-19 pandemic, such as telehealth services or video conferencing tools, that can be utilized in a post-pandemic environment.


Asunto(s)
COVID-19 , Subtipo H1N1 del Virus de la Influenza A , Estudiantes de Medicina , Infección por el Virus Zika , Virus Zika , Humanos , COVID-19/epidemiología , Pandemias , SARS-CoV-2
5.
Acta Trop ; 237: 106724, 2023 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-36270381

RESUMEN

The risk of autochthonous transmission of Zika virus (ZIKV) in Europe greatly depends on the capacity of Aedes albopictus to transmit ZIKV. Experimental studies of the vector competence of European populations of this invasive mosquito species provided contrasting results, that may suggest differences between mosquito populations in ZIKV competence and/or differences in the methodological procedures used on the experimental studies. We systematically reviewed published studies of experimental mosquito infections and show that differences detected in the infection and transmission rates of Ae. albopictus are mostly due to differences between studies in the temperature, the viral load used to inoculate mosquitoes, and the number of days post-exposure (dpe) to the virus at which mosquitoes were analysed. In particular, the proportion of infected mosquitoes was affected by the viral load inoculated and the dpe, whereas the transmission rate was influenced by the viral load and temperature. Thus, the methodological differences used in these studies greatly affect the conclusions obtained regarding the role of Ae. albopictus in Europe as a competent vector for ZIKV and its epidemiological relevance.


Asunto(s)
Aedes , Infección por el Virus Zika , Virus Zika , Animales , Mosquitos Vectores , Saliva
6.
Acta Trop ; 237: 106736, 2023 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-36374844

RESUMEN

Genetic and phylogenetic studies indicated that Zika virus (ZIKV) has evolved into 2 major lineages, the African and Asian. However, ZIKV has been described as a single serotype. This study aimed at assessing the cross-neutralization between ZIKV African and Asian lineages strains. Sixthy-five samples collected in 2007 and 30 samples collected from the same subjects in 2011/2012 in West Africa and positive to neutralizing antibody against ZIKV MR-766 strain (African lineage) were tested against ZIKV H/PF/2013 strain (Asian lineage) by microneutralization assay. All samples showing neutralizing antibodies against MR-766 strain showed also neutralizing activity against H/PF/2013 strain, although with lower titers. This is consistent with about 120 amino acid differences between the two strains. Despite differences in the magnitude of neutralizing activity against different ZIKV strains, all samples showed neutralizing antibody titers considered to be protective.


Asunto(s)
Infección por el Virus Zika , Virus Zika , Humanos , Virus Zika/genética , Sueros Inmunes , Filogenia , Anticuerpos Antivirales , Anticuerpos Neutralizantes
7.
J Virol Methods ; 311: 114638, 2023 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-36328081

RESUMEN

The genus Flavivirus in the Flaviridae contains arthropod born viruses associated with high public health burdens like Zika, Dengue or Yellow fever. Saboya virus (SABV) is an understudied flavivirus grouping in the same genetic sub-group as Yellow Fever Virus (YFV) together with Sepik virus (SEPV) and Wesselbron virus (WSLV). Flavivirus infections are characterized by non-specific clinical presentations resulting in a high risk of misdiagnosis. SABV virus has been shown to circulate in the Sahelian zone and in central Africa. To study this virus we a qRT-PCR system based on TaqMan chemistry was developed to allow rapid and specific detection of SABV. The SABV assay was evaluated on available SABV isolates and others flaviviruses (DENV, ZIKV, YFV, WNV, KEDV). The system reliably detected all used SABV strains without cross amplification of other flaviviruses. In term of sensitivity the SABV assay detect up to 40.25 copies of SABV standard DNA molecule per ul. This system can be easily added to the available panel of arboviruses detection assays as a reliable tool to study virus prevalence in human, vertebrate and insect-vector samples.


Asunto(s)
Virus del Dengue , Flavivirus , Fiebre Amarilla , Infección por el Virus Zika , Virus Zika , Humanos , Flavivirus/genética , Fiebre Amarilla/diagnóstico , Reacción en Cadena en Tiempo Real de la Polimerasa , Virus de la Fiebre Amarilla/genética
8.
Colloids Surf B Biointerfaces ; 221: 112984, 2023 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-36371925

RESUMEN

We report the development of a new nanostructured electrochemical immunosensing platform for the detection of the Zika virus envelope protein (EP-ZIKV). For this, quantum dots (QDs) were explored in combination with screen-printed carbon electrodes (SPCEs) functionalized with a conductor polymeric film, formed from 2-(1H-pyrrol-1-yl)ethanamine (Pyam), and anti-EP DIII ZIKV antibodies. Carboxylated CdTe QDs were synthesized, characterized by optical and structural techniques, and covalently immobilized onto the SPCE/PPyam surface. Then, anti-EP ZIKV antibodies were also covalently conjugated to QDs. All stages of platform assembly were evaluated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The detection of EP-ZIKV was performed by differential pulse voltammetry (DPV). Results indicated that QDs were efficiently immobilized, and did not show oxidation, under the conditions evaluated, for at least 7 months. Anti-EP ZIKV antibodies were effectively immobilized on the PPyam/QDs surface, even after 2 months of electrode storage. The platform enabled the detection of EP-ZIKV with high sensitivity using minimal sample volumes (LOD = 0.1 ng mL-1 and LOQ = 0.4 ng mL-1). The platform was also able to detect EP-ZIKV in spiked serum samples. Moreover, the platform showed specificity, not detecting the EP-DENV 3 nor a mixture of four DENV serotypes antigens. Thus, the proposed combination favored the development of a sensitive immunosensing platform, promising for the detection of Zika in the viremic phase, which also holds potential for transposition to other arboviruses.


Asunto(s)
Técnicas Biosensibles , Compuestos de Cadmio , Puntos Cuánticos , Infección por el Virus Zika , Virus Zika , Humanos , Puntos Cuánticos/química , Virus Zika/metabolismo , Infección por el Virus Zika/diagnóstico , Compuestos de Cadmio/química , Telurio/química , Técnicas Biosensibles/métodos , Biomarcadores/metabolismo
9.
Artículo en Inglés | MEDLINE | ID: mdl-36411078

RESUMEN

BACKGROUND AND OBJECTIVES: Recent outbreaks of Zika virus (ZIKV) in South and Central America have highlighted significant neurologic side effects. Concurrence with the inflammatory neuropathy Guillain-Barré syndrome (GBS) is observed in 1:4,000 ZIKV cases. Whether the neurologic symptoms of ZIKV infection are immune mediated is unclear. We used rodent and human live cellular models to screen for anti-peripheral nerve reactive IgG and IgM autoantibodies in the sera of patients with ZIKV with and without GBS. METHODS: In this study, 52 patients with ZIKV-GBS were compared with 134 ZIKV-infected patients without GBS and 91 non-ZIKV controls. Positive sera were taken forward for target identification by immunoprecipitation and mass spectrometry, and candidate antigens were validated by ELISA and cell-based assays. Autoantibody reactions against glycolipid antigens were also screened on an array. RESULTS: Overall, IgG antibody reactivities to rat Schwann cells (SCs) (6.5%) and myelinated cocultures (9.6%) were significantly higher, albeit infrequent, in the ZIKV-GBS group compared with all controls. IgM antibody immunoreactivity to dorsal root ganglia neurones (32.3%) and SCs (19.4%) was more frequently observed in the ZIKV-GBS group compared with other controls, whereas IgM reactivity to cocultures was as common in ZIKV and non-ZIKV sera. Strong axonal-binding ZIKV-GBS serum IgG antibodies from 1 patient were confirmed to react with neurofascin 155 and 186. Serum from a ZIKV-infected patient without GBS displayed strong myelin-binding and putative antilipid antigen reaction characteristics. There was, however, no significant association of ZIKV-GBS with any known antiglycolipid antibodies. DISCUSSION: Autoantibody responses in ZIKV-GBS target heterogeneous peripheral nerve antigens suggesting heterogeneity of the humoral immune response despite a common prodromal infection.


Asunto(s)
Síndrome de Guillain-Barré , Infección por el Virus Zika , Virus Zika , Humanos , Animales , Ratas , Infección por el Virus Zika/complicaciones , Infección por el Virus Zika/epidemiología , Síndrome de Guillain-Barré/diagnóstico , Inmunoglobulina M , Inmunoglobulina G , Autoanticuerpos
10.
Bioorg Chem ; 130: 106265, 2023 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-36417826

RESUMEN

Zika virus (ZIKV) infection could cause severe neurological complications such as neonatal microcephaly, Guillain-Barré syndrome, and myelitis in adults. No vaccine or therapeutic drug is available for prevention and control of ZIKV infection yet. Based on previously reported anti-ZIKV hit compound 1, a series of novel N-benzoyl or phenylsulfonyl substituted 2-(piperazin-1-yl)methyl-benzonitrile (PMBN) derivatives was designed, synthesized, and investigated for the antiviral activity against ZIKV replication in different cell-based phenotypic assays. The results indicated that N-phenylsulfonyl-PMBN derivative 24 displayed the comparable antiviral activity and higher oral availability than hit compound 1. Meanwhile, mechanism of action study confirmed that compound 24 acts on the early entry stage of ZIKV life cycle. The identification of this new ZIKV entry inhibitor chemotype provided a promising lead for further optimization to develop new drug for ZIKV infection.


Asunto(s)
Inhibidores de Fusión de VIH , Infección por el Virus Zika , Virus Zika , Humanos , Infección por el Virus Zika/tratamiento farmacológico , Internalización del Virus , Antivirales/farmacología , Antivirales/uso terapéutico , Piperazina/uso terapéutico
11.
Adv Neurobiol ; 29: 449-477, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-36255684

RESUMEN

A number of viruses that have caused wide spread concern e.g. Ebola, Zika, and SARS-CoV2 (severe acute respiratory syndrome coronavirus 2 also known as COVID 19) have at various times, become newsworthy as a result of being newly discovered, mutations enabling them to more efficiently infect humans or modern modes of transportation moving them to areas with naive, susceptible populations. As more is learned about the mechanisms whereby these pathogens enter human cells it has become increasingly evident that carbohydrates expressed on the surface of either target cells or the pathogens themselves are essential. Variability in carbohydrate structures as well as the presence of carbohydrate binding receptors (lectins) provides a plethora of potential binding interactions by which infection of cells can occur. Identification of specific lipid- or protein-associated carbohydrates essential for infection provides support for research being done to develop carbohydrate related inhibitors of those interactions. This chapter (1) discusses scenarios for how carbohydrates affect the ability of specific infectious agents to interact with neural cells, (2) gives examples of problems that may result from development of antibodies to carbohydrate antigens found on pathogens that are similar to epitopes expressed on mammalian cells, and (3) provides examples of approaches either in use or under consideration for translational uses of this information.


Asunto(s)
COVID-19 , Infección por el Virus Zika , Virus Zika , Animales , Humanos , SARS-CoV-2 , ARN Viral , Carbohidratos/química , Sitios de Unión , Epítopos , Lectinas , Lípidos , Infección por el Virus Zika/tratamiento farmacológico , Mamíferos
12.
San Salvador; MINSAL; nov. 10, 2022. 23 p. ilus, graf, tab, mapas.
No convencional en Español | LILACS, BISSAL | ID: biblio-1400405

RESUMEN

Resumen de eventos de notificación hasta SE 44/2022. Alertas internacionales. Situación epidemiológica de dengue. Situación epidemiológica de zika y chikungunya. Infección respiratoria aguda. Neumonías. Vigilancia centinela de influenza y otros virus respiratorios. Vigilancia centinela de rotavirus. Enfermedad diarreica aguda. Resumen Epidemiológico quemaduras por pólvora


Summary of notification events up to SE 44/2022. International alerts. Epidemiological situation of dengue. Zika and chikungunya epidemiological situation. Acute respiratory infection. pneumonias. Sentinel surveillance of influenza and other respiratory viruses. Rotavirus sentinel surveillance. Acute diarrheal disease. Epidemiological Summary Gunpowder Burns


Asunto(s)
Epidemiología , Enfermedades Transmisibles , Publicaciones Electrónicas , Virus , Notificación , El Salvador , Infecciones
13.
San Salvador; MINSAL; nov. 04, 2022. 22 p. ilus, graf, tab, mapas.
No convencional en Español | LILACS, BISSAL | ID: biblio-1400403

RESUMEN

Resumen de eventos de notificación hasta SE 43/2022. Alertas internacionales. Situación epidemiológica de dengue. Situación epidemiológica de zika y chikungunya. Infección respiratoria aguda. Neumonías. Vigilancia centinela de influenza y otros virus respiratorios. Vigilancia centinela de rotavirus. Enfermedad diarreica aguda


Summary of notification events until SE 43/2022. International alerts. Epidemiological situation of dengue. Epidemiological situation of zika and chikungunya. Acute respiratory infection. Pneumonias. Sentinel surveillance of influenza and other respiratory viruses. Rotavirus sentinel surveillance. Acute diarrheal disease


Asunto(s)
Epidemiología , Enfermedades Transmisibles , Publicaciones Electrónicas , Virus , Notificación , El Salvador , Infecciones
14.
San Salvador; MINSAL; nov. 04, 2022. 22 p. ilus, graf, tab, mapas.
No convencional en Español | LILACS, BISSAL | ID: biblio-1400400

RESUMEN

Resumen de eventos de notificación hasta SE 42/2022. Alertas internacionales. Situación epidemiológica de dengue. Situación epidemiológica de zika y chikungunya. Infección respiratoria aguda. Neumonías. Vigilancia centinela de influenza y otros virus respiratorios. Vigilancia centinela de rotavirus. Enfermedad diarreica aguda


Summary of notification events until SE 42/2022. International alerts. Epidemiological situation of dengue. Epidemiological situation of zika and chikungunya. Acute respiratory infection. Pneumonias. Sentinel surveillance of influenza and other respiratory viruses. Rotavirus sentinel surveillance. Acute diarrheal disease


Asunto(s)
Epidemiología , Enfermedades Transmisibles , Publicaciones Electrónicas , Virus , Notificación , El Salvador , Infecciones
15.
Pathog Glob Health ; : 1-8, 2022 Oct 31.
Artículo en Inglés | MEDLINE | ID: mdl-36316985

RESUMEN

The state of Ceará, in the Northeast Region of Brazil, presents the simultaneous circulation of Zika (ZIKV), dengue (DENV) and chikungunya (CHIKV) viruses. In 2017 there were a high number of cases of these three arboviruses, especially CHIKV. Here, we detected the presence of arboviruses ZIKV, DENV and CHIKV and their coinfections in women in endemic regions of the city of Fortaleza, Ceará in a post-Zika epidemic year. Sociodemographic and environmental characteristics associated with arbovirus positivity were also analyzed. Women (n = 1289) between 15 and 39 years old were included. RT-qPCR was performed for virus detection and IgM antibody positivity was also analyzed. One hundred and six (8.3%) participants were positive for one or more arboviruses. Monoinfections (76; 5.9%) were distributed between 22 (1.7%) for ZIKV, 39 (3.1%) for DENV and 15 (1.2%) for CHIKV. Co-infections were detected in 30 (2.3%) of the positive participants and one case with triple infection was found. IgM positivity was found in 2.4% of ZIKV RT-qPCR, 9.6% of DENV and 16.3% of CHIKV. RT-qPCR positivity for arboviruses was associated with low socioeconomic class and presence of a water box sealing in the household. A higher positivity to the three viruses occurred in the month with the lowest wind velocity, which was also preceded by the highest peak of rain and humidity. We identified the simultaneous circulation and co-infection of ZIKV, DENV and CHIKV in Fortaleza in a post-Zika epidemic year. We also highlight the need for continuous epidemiological surveillance combined with molecular diagnostic tools.

16.
Gigascience ; 112022 Nov 03.
Artículo en Inglés | MEDLINE | ID: mdl-36329618

RESUMEN

Vector-borne diseases are responsible for more than 17% of human cases of infectious diseases. In most situations, effective control of debilitating and deadly vector-bone diseases (VBDs), such as malaria, dengue, chikungunya, yellow fever, Zika and Chagas requires up-to-date, robust and comprehensive information on the presence, diversity, ecology, bionomics and geographic spread of the organisms that carry and transmit the infectious agents. Huge gaps exist in the information related to these vectors, creating an essential need for campaigns to mobilise and share data. The publication of data papers is an effective tool for overcoming this challenge. These peer-reviewed articles provide scholarly credit for researchers whose vital work of assembling and publishing well-described, properly-formatted datasets often fails to receive appropriate recognition. To address this, GigaScience's sister journal GigaByte partnered with the Global Biodiversity Information Facility (GBIF) to publish a series of data papers, with support from the Special Programme for Research and Training in Tropical Diseases (TDR), hosted by the World Health Organisation (WHO). Here we outline the initial results of this targeted approach to sharing data and describe its importance for controlling VBDs and improving public health.


Asunto(s)
Enfermedades Transmisibles , Infección por el Virus Zika , Virus Zika , Animales , Humanos , Vectores de Enfermedades , Edición
18.
ISME Commun ; 2(1): 107, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-36338866

RESUMEN

The human population has doubled in the last 50 years from about 3.7 billion to approximately 7.8 billion. With this rapid expansion, more people live in close contact with wildlife, livestock, and pets, which in turn creates increasing opportunities for zoonotic diseases to pass between animals and people. At present an estimated 75% of all emerging virus-associated infectious diseases possess a zoonotic origin, and outbreaks of Zika, Ebola and COVID-19 in the past decade showed their huge disruptive potential on the global economy. Here, we describe how One Health inspired environmental surveillance campaigns have emerged as the preferred tools to monitor human-adjacent environments for known and yet to be discovered infectious diseases, and how they can complement classical clinical diagnostics. We highlight the importance of environmental factors concerning interactions between animals, pathogens and/or humans that drive the emergence of zoonoses, and the methodologies currently proposed to monitor them-the surveillance of wastewater, for example, was identified as one of the main tools to assess the spread of SARS-CoV-2 by public health professionals and policy makers during the COVID-19 pandemic. One-Health driven approaches that facilitate surveillance, thus harbour the potential of preparing humanity for future pandemics caused by aetiological agents with environmental reservoirs. Via the example of COVID-19 and other viral diseases, we propose that wastewater surveillance is a useful complement to clinical diagnosis as it is centralized, robust, cost-effective, and relatively easy to implement.

19.
Front Pediatr ; 10: 944818, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-36340716

RESUMEN

Introduction: In a low-income setting with simultaneous presence of Dengue virus, Zika virus, and Chikungunya virus (CHIKV) in the same region, the difficulty of establishing a clinical diagnosis when the molecular test is not a possibility. Thus, it is important to identify signs and symptoms of Chikungunya that can be used to differentiate it from other arboviruses in children. Methods: This is a cross-sectional study, which was developed in Rio de Janeiro State, Brazil, with the analysis of pediatric medical records regarding arboviruses. Considering that the population had already been exposed to Dengue and Zika viruses and were experiencing the first notification of the CHIKV. The ethics committee approved this research, and all those legally responsible for the children signed the consent form. Results: In total, 159 children were seen of which 98 were suspected CHIKV cases, and 51 had their diagnosis confirmed with reagent IgM/IgG for CHIKV. The symptoms that the pediatric population with CHIKV presented most often were fever (90.2%), arthralgia (76.5%), and exanthema (62.7%) in both suspected and confirmed cases of Chikungunya. Thus, CHIKV in those children presents a clinical profile similar to those found in other studies referring to adults. Additionally, only arthralgia and a high aspartate transaminase were related to the positivity of serology for Chikungunya. Conclusions: This study describes the signs and symptoms of CHIKV exhibited in the pediatric population with a mild and moderate presentation similar to the findings in the adult during an epidemic experienced in a population vulnerable to CHIKV.

20.
Am J Trop Med Hyg ; 2022 Nov 07.
Artículo en Inglés | MEDLINE | ID: mdl-36343593

RESUMEN

The isolation of Zika virus (ZIKV) from serum of suspected human cases for diagnostic purposes can be challenging due to infrastructure constraints of laboratory testing technology. Therefore, as an alternative method, the objective of this study was to evaluate a random sample of oropharyngeal swabs for the diagnosis of ZIKV infection among patients with symptoms of arboviral and respiratory illness. The results revealed that ZIKV RNA could be detected by a reverse transcriptase polymerase chain reaction (RT-PCR) assay and isolated from oropharyngeal swabs from five of 38 samples, but serum samples from the same patients were negative for ZIKV by a variety of laboratory diagnostic approaches including RT-PCR and viral isolation followed by immunofluorescence assays. The findings suggested that the molecular detection and isolation of ZIKV in oropharyngeal swab warrants further study for consideration as an improved diagnostic procedure.

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