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1.
Environ Sci Pollut Res Int ; 28(44): 62216-62234, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34184231

RESUMO

Internal combustion engines are the inevitable prime movers in the contemporary engineering era. The suitability of proper bio-fuel and their blends plays a vital role in engine behaviour. This study aims to select smart fuel opus depending on Aegle marmelos (AM) fuel properties with nano additive blends for diesel engines by using intelligent hybrid decision-making tools. Physicochemical properties of CuO and novel graphene nano sheets added bio-oil combinations were studied. The assessment of an appropriate blend depends on the analysis of fuel properties. The Fuzzy Analytical Hierarchy Process (FAHP) integrated with Grey relational analysis (GRA) was employed for optimum fuel blend selection. The FAHP model was used to identify the criteria weights, whereas GRA was hired to rank alternative fuel blends. Pairwise analysis and ranking of the alternatives were compared to get the optimum fuel blend through FAHP and GRA amalgamation. The addition of nanoparticles enhanced engine performance and reduced emission. The obtained ascending order of preference of the bio-oil blends from FAHP and GRA analysis is AC15G15>AG30>AC30>A10>A20. From FAHP, GRA, and engine test results, it is observed that AC15G15 opus is the most suitable fuel blend for diesel engines. Lower fuel consumption (0.37 kg/kW hr) and emissions (CO level of 0.21%, which is 0.34% for diesel, HC value of 134 ppm, which is 184 ppm for diesel) of AC15G15 aids in contributing towards a green and clean environment.


Assuntos
Aegle , Gasolina , Biocombustíveis , Emissões de Veículos
2.
J Food Biochem ; 45(4): e13704, 2021 04.
Artigo em Inglês | MEDLINE | ID: mdl-33719131

RESUMO

Hexavalent chromium [Cr (VI)] is highly toxic compared to other valence states of chromium. In the process of metabolic reduction, Cr (VI) converts to trivalent chromium. Aegle marmelos (Bael), a sacred plant of India and its fruits are being consumed as traditional formulations against various diseases such as ulcer, gastric mucosal damage, inflammations, febrile delirium, acute bronchitis, anxiety, etc. The present study assessed the protective effects of marmelosin (MAR) from Aegle marmelos against K2 Cr2 O7 -induced toxic effects in HepG2 cell line through its antiapoptotic mechanism. Results of the study revealed that pretreatment of MAR ameliorated cell viability, mitochondrial damage, and DNA damage induced by K2 Cr2 O7 in HepG2 cell line as evidenced by cell morphology, MTT, LDH, and MMP assays. Pretreatment of MAR attenuated K2 Cr2 O7 -induced oxidative stress by downregulating intracellular ROS and RNS. Further, pretreatment of MAR significantly downregulated K2 Cr2 O7 -induced apoptotic markers, such as Bax, Caspase 3, and Gadd45. Our results suggested that application of marmelosin could be beneficial in ameliorating chromium-induced apoptotic cell death by suppressing oxidative stress and regulating excessive DNA damage. PRACTICAL APPLICATIONS: The study focused on protective mechanism of marmelosin from Aegle marmelos against chromium-induced oxidative stress for the first time. In this research, we reported that marmelosin effectively ameliorated K2 Cr2 O7 -induced morphological changes such as oxidative stress and apoptotic cell death by regulating Gadd45, Bcl-2, Bax, and Caspase 3 gene expressions, and inhibition of intracellular ROS and RNS. The study provides a better understanding of the pharmacological mechanisms of Aegle marmelos and its bioactive compound, that is, marmelosin in the management of intoxication of heavy metals associated with excessive DNA damage.


Assuntos
Aegle , Apoptose , Linhagem Celular , Cromo , Furocumarinas , Estresse Oxidativo , Extratos Vegetais/farmacologia
3.
Phytother Res ; 35(4): 1887-1907, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33159390

RESUMO

Aegle marmelos L. (bael) is a fruit tree of Rutaceae family, widely grown all over the world. This plant is gaining popularity because of its nutrient-rich fruits and immense traditional medicinal usage and pharmacological properties. The health promotive and protective effect of bael fruit is accounted by fibers, carotenoids, phenolics, terpenoids, coumarins, flavonoids, and alkaloids. The curative relevance of these compounds has been assessed by various in vivo and in vitro studies. Fruit shows numerous possible health benefits, namely, radio-protective effects, peroxidation, antibacterial, inhibition of lipid, antidiarrheal, gastroprotective, antiviral, antidiabetic, anti-ulcerative colitis, cardioprotective, free-radical scavenging (antioxidant) and hepatoprotective effects. The health benefits of bael are not only limited to edible portion (fruit), but it also extends to nonedible portion (root, trunk, bark, leaf, flower and seed) having comparable biologically active compounds. Increasing awareness about the role of diet among health-conscious consumers for human well-being has increased the interest in functional foods thereby exploration of the functional attributes of various underutilized plants is being reaffirmed and various sources are emerged out as suitable food material for processing industry. The various scientific reports collected from different bibliometric sources suggested that A. marmelos and its bioactive constituents could play a vital role in the prevention of several chronic and degenerative diseases associated with oxidation stress. This review emphasis on recent scientific evidences on nutrition and bioactive profile of A. marmelos, health benefits along with clinical and nonclinical trials of various phytoconstituents and A. marmelos potential in food processing industry for various food products. Our study suggests that this plant does indeed have pharmacological properties of interest, however, further extensive research is needed to establish a potential strategy that can balance the pharmacological and toxic effects of bael.


Assuntos
Aegle/química , Dietoterapia/métodos , Medicina Herbária/métodos , Extratos Vegetais/química , Humanos
4.
Int J Biol Macromol ; 167: 587-594, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-33278437

RESUMO

Ferulic acid was chemically grafted onto the arabinogalactan protein of Aegle marmelos fruit gum using 1,1'-carbonyldiimidazole as coupling reagent. Thus, grafted polysaccharides with different degrees of substitution were prepared and then characterized by gas chromatography/mass spectrometry, size exclusion chromatography, and ultraviolet-visible, infra-red, and nuclear magnetic resonance spectroscopic investigations. Fluorescence spectroscopic investigation showed hydrophobic microdomain formation in grafted polymers. The antioxidant activities of the derivatives, as determined by the 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl radical assay, were strong and increases with increasing the degree of feruloylation. Compared to parental arabinogalactan protein (K = 2.38 × 106 M-1), these grafted polymers bind more strongly with ß-lactoglobulin (K = 11.4 × 106 M-1 and 8.19 × 106 M-1). Given that gum polysaccharides are valuable component in functional foods, synthesis of antioxidative graft polymer possessing good compatibility with ß-lactoglobulin may have important implication.


Assuntos
Antioxidantes/farmacologia , Ácidos Cumáricos/farmacologia , Lactoglobulinas/metabolismo , Mucoproteínas/química , Aegle/metabolismo , Antioxidantes/química , Cromatografia em Gel , Ácidos Cumáricos/química , Interações Hidrofóbicas e Hidrofílicas , Espectroscopia de Ressonância Magnética , Proteínas de Plantas/química , Ligação Proteica , Espectroscopia de Infravermelho com Transformada de Fourier
5.
J Evid Based Integr Med ; 25: 2515690X20971586, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33356558

RESUMO

An extensive study has been made to identify, document, and investigate the ethnomedicinal plants used by Rakhine ethnic minorities in Patuakhali and Barguna District of southern Bangladesh for the term of April 2018 to June 2019. In this article, we have focused on the Rakhine population trends, management concerns, and some actions for conserving the Rakhine population diversity in the study area. In this study, we have identified the locations where Rakhine population lives in Patuakhali and Barguna districts. A total of 86 plant species belonging to 71 genera and 43 families were reported to be used for treating more than 57 various physical ailments under 14 illness categories from the study area. For each of the species, the botanic name, common name, Rakhine name, family, habit, parts used and traditional medicinal uses of the plant species have been presented. The maximum numbers of ethnomedicinal plant species were utilized to treat gastrointestinal complaints (43) taken after by the treatment of dermatological issues (36). The highly cited (75.60%) plant species were found to be Ananas comosus and Aegle marmelos used for gastro-intestinal (Stomach pain, indigestion, and dysentery) digestive disorders and subsequently followed by Colocasia esculenta (70.73%) used for cut, bleeding and wound healing. The results of this study have shown that Rakhine indigenous communities still depend on conventional plant-based medication to remedy various diseases and therapeutic purposes in the study area. Our findings have also shown that despite there have adequate phytodiversity in the natural habitat of the study area but the number of Rakhine population has been declining significantly day-by-day. As an ultimate result, we have lost the plant-based traditional medicinal knowledge of Rakhine indigenous communities in Bangladesh. As a rich source of traditional knowledge and cultural diversity, it calls for urgent initiatives to conserve the cultural heritage of the Rakhine community as well as the diversity of Rakhine ethnic group.


Assuntos
Grupos Étnicos , Etnobotânica , Medicina Tradicional , Aceitação pelo Paciente de Cuidados de Saúde , Fitoterapia , Extratos Vegetais/uso terapêutico , Plantas Medicinais , Aegle , Ananas , Bangladesh , Colocasia , Doenças do Sistema Digestório/tratamento farmacológico , Documentação , Etnofarmacologia , Humanos , Conhecimento , Dermatopatias/tratamento farmacológico , Inquéritos e Questionários , Cicatrização
6.
Sci Rep ; 10(1): 18016, 2020 10 22.
Artigo em Inglês | MEDLINE | ID: mdl-33093498

RESUMO

Breast cancer is among most common form of cancer worldwide. It is also the major cause of death in female cancer patient around the world. Despite various therapeutic measures, it remains associated with high mortality rate. Aegle marmelos (L.) Correa has been extensively used in Indian medicine system Ayurveda, due to its various medicinal properties. However, there are very limited reports regarding its anticancer activity. Thus, the present research work has been aimed to study the anticancer activity of Aegle marmelos fruit extract on 7,12-dimethylbenz(a)anthracene (DMBA) induced breast cancer in rats. Female Charles Foster rats, 55-60 days old weighing around (150 ± 10 g) were used for the study and were induced DMBA (20 mg/mL dissolved in Olive oil) orally. After the development of breast tumors (about 0.5 cm), the rats were treated with Aegle marmelos ethanolic fruit pulp extract (200 mg/kg b.w./day) orally for 5 weeks and then volume of tumor was measured. Aegle marmelos treatment showed significantly reduced mammary tumor volume (P < 0.05), along with significant reduction (P < 0.0001) in the different serum biomarkers such as TNF-α level, serum malondialdehyde (MDA) level and glucose levels. Significant (P < 0.0001) improvement in both, the kidney and liver serum biomarker parameters were also observed after the treatment with Aegle marmelos ethanolic fruit pulp extract. From the entire study, taking everything into account it can be interpreted that Aegle marmelos ethanolic fruit pulp extract possesses anti-proliferative activity by suppressing the progression of breast tumors in rat model. The plant extract also possesses hepato-renal protective effect. Hence, it can be targeted as novel and safe anti-cancer drug against breast cancer.


Assuntos
Aegle/química , Antracenos/toxicidade , Frutas/química , Peroxidação de Lipídeos/efeitos dos fármacos , Neoplasias Mamárias Experimentais/tratamento farmacológico , Fitoterapia , Piperidinas/toxicidade , Extratos Vegetais/farmacologia , Animais , Feminino , Malondialdeído/metabolismo , Neoplasias Mamárias Experimentais/induzido quimicamente , Neoplasias Mamárias Experimentais/patologia , Ratos
7.
J Complement Integr Med ; 18(1): 51-57, 2020 Aug 03.
Artigo em Inglês | MEDLINE | ID: mdl-32745070

RESUMO

OBJECTIVES: Aqueous leaves extracts of Murraya koenigii (M. koenigii) and Aegle marmelos (A. marmelos) were prepared and effect of the extracts on inhibiting alpha-amylase playing essential roles on converting starch into glucose have been examined using in vitro assays. METHODS: Alpha amylase inhibitory assay was used to asses the in vitro antidiabetic activity of the extracts. Gas chromatography-mass spectrometry (GC-MS) analysis was performed to identify the volatile molecules of the extracts. Identified molecule were converted as ligand and docked against human pancreatic α-amylase (0.95 Å; PDB ID: 5U3A) using Autodock tool. RESULTS: The data analyzes suggested that the alpha-amylase inhibition potential of the extract obtained from M. koenigii was stronger than that of the A. marmelos at low concentrations (<1 mg/mL), whereas both the extracts depicted similar inhibition effects on the enzyme at high concentration (>1 mg/mL). The phytochemicals present in both the plant extracts were identified by using their respective GC-MS data and the data analyzes revealed that the extracts of M. koenigii and A. Marmelos seemed to consist of about 20 and 24 diverse chemical molecules, respectively. Through the molecular docking studies, azulene of M. koenigii and hydroxycyclodecadiene of A. marmelos showed higher binding affinity on alpha-amylase. CONCLUSIONS: Concentration-dependent alpha-amylase inhibition effects of the extracts were observed and M. koenigii contains more alpha-amylase inhibitory effects due to the presence of azulene. This is primary lead to find out the better anti diabetic natural based drug to the society after clinical trial.


Assuntos
Aegle/química , Inibidores Enzimáticos/farmacologia , Murraya/química , Extratos Vegetais/farmacologia , alfa-Amilases/antagonistas & inibidores , Humanos , Hipoglicemiantes/farmacologia , Simulação de Acoplamento Molecular , Compostos Fitoquímicos/farmacologia , Folhas de Planta/química
8.
Curr Drug Metab ; 21(7): 525-533, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32651962

RESUMO

BACKGROUND: Oxidative stress is among the main causes of metabolic disorders. Hence, there is a need to discover potent antioxidants for therapeutic applications. OBJECTIVE: The objective of this study has been to investigate the phytoconstituents of the methanolic extract of the hard shell of Aegle marmelos fruit and their antioxidant potential. METHODS: Methanolic extract was fractionated using different solvents by liquid-liquid extraction. Characterization of the phytoconstituents was done by using phytochemical tests and GC-MS analysis. The free radical scavenging activity, total reducing power, lipid peroxidation inhibition and cell protection assays against oxidative stress were performed with methanolic extract and its fractions. RESULTS: Therapeutically significant class of compounds, for example, polyphenols, glycosides and sterols were revealed in the hard-shell extract. Differential separation of compounds was achieved by liquid-liquid extraction using different solvents. Six compounds: 4-Hydroxybenzeneacetic acid; 5-Oxo-pyrrolidine-2-carboxylic acid methyl ester; 1-[3-Methyl-3-Butenyl] Pyrrolidine; Trans-sinapyl alcohol; 5-[Hydroxymethyl]-2-furaldehyde and 2,4- Dihydroxy-2,5-dimethyl-3[2H]-furan-3-one, identified in the fruit-shell extract, are being reported for the first time from this plant. Strong antioxidant potential of the extract was evident from efficient scavenging of free radicals. The extract also conferred protection to yeast cells against oxidative damage. CONCLUSION: Results showed that the hard shell of the Aegle marmelos fruit was a potent source for antioxidant compounds, which can be developed for therapeutic applications in the control and management of metabolic diseases.


Assuntos
Aegle , Antioxidantes , Antioxidantes/análise , Antioxidantes/química , Antioxidantes/farmacologia , Radicais Livres/química , Frutas/química , Estresse Oxidativo/efeitos dos fármacos , Compostos Fitoquímicos/análise , Compostos Fitoquímicos/química , Compostos Fitoquímicos/farmacologia , Extratos Vegetais/análise , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Saccharomycetales/efeitos dos fármacos
9.
J Sci Food Agric ; 100(15): 5596-5602, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32608520

RESUMO

BACKGROUND: A lack of knowledge about the dynamic rheological properties and color degradation of bael fruit pulp gives rise to many problems during thermal processing for the preparation of bael fruit products. In this research, the effects of processing temperature (30-75 °C) on dynamic rheology and color degradation kinetics of bael fruit pulp were studied and analyzed. RESULTS: Bael fruit pulp showed more elastic behavior than viscous behavior by analyzing the storage modulus, loss modulus and phase angle over oscillatory frequency range 0.06-300 rad s-1 . The dynamic rheological study classified the bael fruit pulp as a weak gel (G' > G″). The values of first-order degradation rate constant (k) were determined to be 0.07, 0.011, 0.018 and 0.023 for 30, 45, 60 and 75 °C, respectively. The first-order color degradation kinetics of bael fruit pulp has given a higher value of activation energy (Ea = 23.83 kJ mol-1 ) with coefficient of determination (R2 ) of 0.99, which indicated a higher sensitivity for treatment temperature and time during processing. CONCLUSIONS: The results obtained would allow simplification of processes for easier formulation of quality products from bael fruit pulp. This study has the potential to increase the processing ability of bael fruit, which will encourage a greater production of bael fruits. © 2020 Society of Chemical Industry.


Assuntos
Aegle/química , Manipulação de Alimentos/métodos , Frutas/química , Pigmentos Biológicos/química , Cor , Géis/química , Cinética , Reologia , Temperatura
10.
PLoS One ; 15(5): e0233609, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32442219

RESUMO

Aegle marmelos L. (Bael) is a native tree fruit species in the Indian subcontinent and Southeast Asia. Bael is a popular fruit because of its significant nutritional and medicinal properties. However, bael is an underutilized fruit species in Sri Lanka. Thus, Fruit Crop Research and Development Station of the Department of Agriculture of Sri Lanka has selected five elite bael accessions (Beheth Beli, Paragammana, Mawanella, Rambukkana, and Polonnaruwa-Supun). We assessed these five accessions for the variation of the fruit size, pulp, organoleptic preference, elemental properties, genetic diversity, and evolutionary history. The fruits at the golden-ripe stage were collected during the peak fruiting seasons in 2015, 2016, and 2017. The fruit size, pulp, shell thickness, and seed size were measured and subjected to the General Linear Model (GLM) and Principal Component (PC) Analyses. The fruit pulp was distributed among a group of 30 taste-panelists to rank for the parameters: external appearance, flesh color, aroma, texture, sweetness, and overall preference. The rank data were subjected to association and PC analyses. The elemental contents of the fruit pulp samples were measured using Inductively coupled plasma mass spectrometry and subjected to GLM and PC analyses. We observed a significant diversity in fruit size, organoleptic preference, and elemental contents among bael accessions. Rambukkana and Polonnaruwa-Supun yield the biggest and most preferred fruits. We used trnH-psbA, atpB-rbcL spacer, matk-trnT spacer, and trnL markers to construct phylogenies. Sri Lankan bael split from an Indian counterpart, approximately 8.52 MYA in the Pliocene epoch. However, broader germplasm of Indian bael must be assessed to see the presence of any independent evolution within Sri Lanka.


Assuntos
Aegle/química , Frutas/química , Extratos Vegetais/química , Aegle/classificação , Filogenia , Sri Lanka
11.
Int J Biol Macromol ; 149: 962-975, 2020 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-32006582

RESUMO

In the present work, the chemical composition of the Chilean freshwater crab Aegla cholchol exoskeleton was studied for the first time. α-Chitin was isolated from three main body parts (pincers, legs, carapace), and its content ranged from 9.0-10.4% (w/w). Moreover, chitosan was extracted by alkaline treatment at different temperature and time regimes. Aegla cholchol exoskeleton, chitin and chitosans were characterized by FTIR, TGA, and SEM. Chemical structure of chitin and chitosan was confirmed by NMR spectroscopy. Chitosan molecular weight determinations were carried out by GPC. The obtained chitosan samples had a degree of N-acetylation (DA) between 4 and 15% and molecular weight (Mw) in the range of 65-201 kDa. The antifungal activity of the chitosan samples and the chitooligomer were tested toward twenty isolated clinical strains of Candida yeast. Chitosan with lower DA (4%) and higher molecular weight showed the lowest minimum inhibitory concentration (MIC) values, which was evidenced by the highest antifungal activity toward Candida albicans, Candida tropicalis, Candida glabrata, and Candida parapsilosis. Results suggest that Aegla cholchol is an excellent natural source for production of bioactive materials with potential applications in the health system, to prevent infections associated with Candida strains.


Assuntos
Aegle/química , Antifúngicos/química , Antifúngicos/farmacologia , Candida/efeitos dos fármacos , Quitina/química , Quitina/farmacologia , Quitosana/química , Quitosana/farmacologia , Fenômenos Químicos , Quitina/isolamento & purificação , Quitosana/isolamento & purificação , Lipídeos/química , Peso Molecular , Nitrogênio/química , Análise Espectral , Termogravimetria
12.
J Ethnopharmacol ; 245: 112155, 2019 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-31449858

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Traditional Indian medicine has utilized Aeglemarmelos (L.) Corr. commonly called as bael in several indigenous systems against various diseases. Bioactive components isolated from various plant parts of A. marmelos were used in ethno-medicine. More precisely they are known for its antiviral property against various human and animal viruses. AIM OF THE STUDY: The study was conducted to investigate the antiviral activity of A.marmelos against Bombyx mori nucleopolyhedrovirus (BmNPV). MATERIALS AND METHODS: Among the various crude extracts tested, hexane extracts of leaves of A. marmelos with promising anti-BmNPV activity was subjected to bioactivity guided fractionation based on column chromatography. Out of 40 fractions obtained from the fractionation, fractions showing similar TLC profiles were pooled into 14 fractions. A fraction with potential activity was used to purify a molecule with anti-BmNPV activity. This molecule was characterized through structural and functional analyses. RESULTS: The functionally and structurally characterized molecule in the fraction with prospective anti-BmNPV activity revealed a single crystal compound 'seselin' (8, 8-dimethyl pyrido oxazine-2-one). CONCLUSION: It is therefore understood that this seselin compound could be used as a natural medicine for the management of NPV infection in the silkworm larvae under commercial conditions after suitable field evaluations.


Assuntos
Aegle , Antivirais/uso terapêutico , Bombyx/efeitos dos fármacos , Cumarínicos/uso terapêutico , Larva/efeitos dos fármacos , Animais , Antivirais/farmacologia , Bombyx/virologia , Cumarínicos/farmacologia , Trato Gastrointestinal/efeitos dos fármacos , Trato Gastrointestinal/patologia , Hemócitos/efeitos dos fármacos , Proteínas de Insetos/metabolismo , Larva/virologia , Simulação de Acoplamento Molecular , Nucleopoliedrovírus , Extratos Vegetais/farmacologia , Extratos Vegetais/uso terapêutico , Folhas de Planta
13.
Biofactors ; 45(6): 867-879, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31424600

RESUMO

There is a growing awareness in herbal medications as they are usually safe and devoid of significant adverse effects. Auraptene is a natural bioactive monoterpene coumarin ether and is consumed all over the world. There is growing evidence of the therapeutic benefits of auraptene. Auraptene, also known as auraptene and 7-geranyloxycoumarin, is a bioactive monoterpene coumarin from Rutaceae family, which is isolated from Citrus aurantium (Seville orange) and Aegle marmelos (bael fruit). Auraptene is a highly pleiotropic molecule, which can modulate intracellular signaling pathways that control inflammation, cell growth, and apoptosis. It has a potential therapeutic role in the prevention and treatment of various diseases due to its anti-inflammatory and antioxidant activities as well as its excellent safety profile. In the present article, various pharmacological and therapeutic effects of auraptene were reviewed. Different online databases using keywords such as auraptene, therapeutic effects and pharmacological effects were searched until the end of September 2018, for this purpose. Auraptene has been suggested to be effective in the treatment of a broad range of disorders including inflammatory disorders, dysentery, wounds, scars, keloids, and pain. In addition, different studies have demonstrated that auraptene possesses numerous pharmacological properties including anti-inflammatory, anti-oxidative, anti-diabetic, anti-hypertensive and anti-cancer as well as neuroprotective effects. The present review provides a detailed survey of scientific researches regarding pharmacological properties and therapeutic effects of auraptene.


Assuntos
Anti-Inflamatórios/uso terapêutico , Cumarínicos/uso terapêutico , Inflamação/tratamento farmacológico , Aegle/química , Anti-Inflamatórios/química , Apoptose/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Citrus/química , Cumarínicos/química , Humanos , Transdução de Sinais/efeitos dos fármacos
14.
Chem Biol Interact ; 310: 108750, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31319076

RESUMO

Osteoporosis is a major health concern occurring to the aging adult population across the globe. Currently, there is an increasing demand for treatment of osteoporosis with plant-based medicines. In the present study, we report that heraclenin was extracted and purified from unripe fruit portion of Bael (Aegle marmelos Corr.) using silica gel column chromatography. The identification and characterization of heraclenin were carried out by UV-Vis, HPLC, LC-MS, NMR, FT-IR, and XRD analyses. The standardized purification method recorded a yield efficiency of 42% heraclenin microcrystals with 99% purity from bael fruit. SEM image revealed the shape of the purified compound to be an orthorhombic-sphenoid prism. Cytotoxicity studies indicated that heraclenin-treatment did not alter cell viability in mouse mesenchymal stem cells (mMSCs, C3H10T1/2). The mRNA expression of Runx2, a bone transcription factor was found to be stimulated by heraclenin in these cells. At the cellular level, heraclenin-treatment enhanced osteoblast differentiation and mineralization in mMSCs. Thus, these results suggested that heraclenin purified from bael fruit has an osteogenic effect, indicating its potential towards bone regeneration.


Assuntos
Aegle/química , Furocumarinas/farmacologia , Células-Tronco Mesenquimais/efeitos dos fármacos , Osteogênese/efeitos dos fármacos , Animais , Diferenciação Celular/efeitos dos fármacos , Sobrevivência Celular , Subunidade alfa 1 de Fator de Ligação ao Core/genética , Furocumarinas/isolamento & purificação , Camundongos , Osteoblastos/citologia , RNA Mensageiro/metabolismo , Espectrofotometria
15.
Fitoterapia ; 138: 104266, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31302251

RESUMO

Aegle marmelos (L.) Corr. (Rutaceae), also known as Bael tree, is an herbal traditional remedy in the South East Asia. In the present work, the leaf essential oil distilled from a population collected in Nepal was analyzed for the chemical composition by GC-MS showing different phytochemical constituents compared with literature data. The obtained oil was rich in sesquiterpenes, mainly ß-Caryophyllene (26%), whereas monoterpenes, known in literature as the major components, were present in little amounts. Due to richness in sesquiterpenes which are promising as anticancer drugs, the oil was tested against several human tumor cell lines namely pancreatic (PSN-1), colon (LoVo), lung (H157) and ovarian (2008) cells showing IC50 of 5.6 µg/mL, 6.5 µg/mL, 6.7 µg/mL and 2.3 µg/mL, respectively. In vivo distribution of oil was studied with a dose of 41.5 mg/kg in mice allowing the quantification of ß-Caryophyllene, α-Humulene, γ-Muurulene and ar-Curcumene at 30 and 60 min after oral administration. Sesquiterpene were found in higer amount in, liver, kidney and heart whereas lung and blood contained lower levels. The tissue distribution study demonstrated that active sesquiterpenoids of the oil can efficiently reach different organs.


Assuntos
Aegle/química , Antineoplásicos Fitogênicos/farmacologia , Óleos Voláteis/farmacologia , Sesquiterpenos/farmacologia , Animais , Antineoplásicos Fitogênicos/isolamento & purificação , Linhagem Celular Tumoral , Feminino , Humanos , Camundongos , Nepal , Óleos Voláteis/química , Compostos Fitoquímicos/isolamento & purificação , Compostos Fitoquímicos/farmacologia , Folhas de Planta/química , Sesquiterpenos Policíclicos , Sesquiterpenos/isolamento & purificação
16.
Int J Biol Macromol ; 130: 527-535, 2019 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-30794900

RESUMO

The potential of hydrothermal technology was utilized for the preparation of promising adsorbents in order to overcome the troubles of methemoglobinemia (blue baby syndrome) and eutrophication which is caused by excess nitrate (NO3-) and phosphate (PO43-) ions in water. Hence, in the present investigation, the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads was prepared by both in situ precipitation (In situ) and hydrothermal (Hydro) methods for NO3- and PO43- adsorption. The hydro supported La2O3AM@CS composite beads hold an enhanced nitrate and phosphate sorption capacity (SC) of 27.84 and 34.91 mg/g than the other adsorbents prepared by in situ method. The characterization studies of the adsorbents such as FTIR, XRD, SEM and BET analysis were explored in detail. In batch scale, the adsorption affecting parameters such as contact time, pH, adsorbent dosage, initial adsorbate strength, competing anions and temperature was optimized. The fitted experimental data of various isotherms and thermodynamic parameters supports the feasible nature of NO3- and PO43- adsorption system. The field trial investigations and reuse of La2O3AM@CS composite beads were also executed.


Assuntos
Aegle/química , Quitosana , Lantânio , Nitratos , Óxidos , Fosfatos , Adsorção , Quitosana/química , Concentração de Íons de Hidrogênio , Íons , Lantânio/química , Nanocompostos/química , Nanocompostos/ultraestrutura , Nitratos/química , Óxidos/química , Fosfatos/química , Análise Espectral , Temperatura , Termodinâmica
17.
Life Sci ; 221: 196-203, 2019 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-30771313

RESUMO

AIMS: Aegle marmelos (L.) Correa (A. marmelos) has been used in Ayurvedic medicine as a brain tonic however its neuroprotective effect against streptozotocin (STZ) induced cognitive impairment and oxidative stress has not been reported yet in vivo. Therefore, the present study was attempted to investigate the neuroprotective potential of ethanolic extract of A. marmelos leaves (AME) on STZ induced memory impairment in male rats. MAIN METHODS: Albino Wistar rats were pre-treated orally with AME at the doses 200 and 400 mg/kg for two weeks, followed by intracerebroventricular (i.c.v.) injection of STZ (3 mg/kg) on day 1 and 3. Two weeks after STZ administration, behavioural parameters were monitored using Morris water maze task. Biochemical and histopathological studies were carried out after three weeks of STZ administration. The levels of oxidative stress markers (malondialdehyde (MDA), glutathione, nitrite, catalase) neuroinflammatory mediators; tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) and acetylcholinesterase (AChE) activity were estimated in hippocampus of rat brain. Donepezil (5 mg/kg) was taken as a standard drug. KEY FINDINGS: The levels of MDA, nitrite, TNF-α and IL-6 were significantly increased while glutathione levels were significantly decreased in hippocampus of STZ-treated rats. Further, a significant decrease in the activity of catalase and increase in AChE activity was observed indicating cholinergic hypofunction and neuronal damage in STZ-treated animals. All these alterations were significantly ameliorated by AME in a dose dependent manner. SIGNIFICANCE: The neuroprotective potential of A. marmelos against STZ induced oxidative stress and cognitive deficit in rats indicates its therapeutic value in Alzheimer's disease (AD).


Assuntos
Aegle/metabolismo , Disfunção Cognitiva/tratamento farmacológico , Extratos Vegetais/uso terapêutico , Acetilcolinesterase , Animais , Cognição/efeitos dos fármacos , Transtornos Cognitivos/tratamento farmacológico , Hipocampo , Injeções Intraventriculares , Interleucina-6 , Masculino , Malondialdeído , Aprendizagem em Labirinto/efeitos dos fármacos , Medicina Tradicional do Leste Asiático , Transtornos da Memória/tratamento farmacológico , Fármacos Neuroprotetores , Estresse Oxidativo/efeitos dos fármacos , Extratos Vegetais/metabolismo , Folhas de Planta , Ratos , Ratos Wistar , Estreptozocina/efeitos adversos , Estreptozocina/farmacologia , Fator de Necrose Tumoral alfa
18.
Environ Sci Pollut Res Int ; 26(7): 6980-7004, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30645748

RESUMO

This research focuses on the detailed experimental assessment of compression ignition (CI) engine behavior fuelled with Aegle marmelos (AM) seed cake pyrolysis oil blends. The study on effects of engine performance and emission a characteristic was designed using L25 orthogonal array (OA). These multi-objectives were normalized through gray relational analysis (GRA). Likewise, the principal component analysis (PCA) was performed to assess the weighting values respective to every performance and emission characteristics. The variability induced by using the input process parameters was allocated using analysis of variance (ANOVA). Hence, GRA-coupled PCA were employed to determine the optimal combination of CI engine control factors. The greater combination of engine characteristics levels were selected with F5 and W5. The higher brake thermal efficiency (BTE) have been obtained for F20 fuel as 22.01% at peak engine load, which is 11.43% for diesel. At peak load condition, F20 fuel emits 14.99% lower HC and 18.52% lower CO as compared to diesel fuel. The improved engine performance and emission characters can be attained by setting the optimal engine parameter combination as F20 blend at full engine load condition. The validation experiments show an improved average engine performance of 67.36% and average lower emission of 64.99% with the composite desirability of 0.8458.


Assuntos
Aegle/química , Gasolina/análise , Modelos Químicos , Pirólise , Biocombustíveis , Análise de Componente Principal , Emissões de Veículos
19.
J Biomol Struct Dyn ; 37(1): 195-209, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29308712

RESUMO

Quinolone synthase from Aegle marmelos (AmQNS) is a Rutacean-specific plant type III polyketide synthase that synthesizes quinolone, acridone, and benzalacetone with therapeutic potential. Simple architecture and broad substrate affinity of AmQNS make it as one of the target enzymes to produce novel structural scaffolds. Another unique feature of AmQNS despite its high similarity to acridone forming type III polyketide synthase from Citrus microcarpa is the variation in the product formation. Hence, to explore the characteristic features of AmQNS, an in-depth sequence and structure-based bioinformatics analyses were performed. Our studies indicated that AmQNS and its nearest homologs have evolved by a series of gene duplication events and strong purifying selection pressure constrains them in the evolutionary process. Additionally, some amino acid alterations were identified in the functionally important region(s), which can contribute to the functional divergence of the enzyme. Prediction of favorable amino acid substitutions will be advantageous in the metabolic engineering of AmQNS for the production of novel compounds. Furthermore, comparative modeling and docking studies were utilized to investigate the structural behavior and small molecule interaction pattern of AmQNS. The observations and results reported here are crucial for advancing our understanding of AmQNS's phylogenetic position, selection pressure, evolvability, interaction pattern and thus providing the foundation for further studies on the structural and reaction mechanism.


Assuntos
Aegle/química , Desenho de Fármacos , Ligantes , Modelos Moleculares , Policetídeo Sintases/química , Relação Quantitativa Estrutura-Atividade , Substituição de Aminoácidos , Evolução Biológica , Simulação de Acoplamento Molecular , Simulação de Dinâmica Molecular , Estrutura Molecular , Mutação , Filogenia , Policetídeo Sintases/classificação , Policetídeo Sintases/genética , Ligação Proteica , Seleção Genética
20.
Nat Prod Res ; 33(15): 2236-2242, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30067085

RESUMO

Aegle marmelos is a plant species native to India. Commercially available food products such as jam, jelly, candy, squash etc. are prepared from ripe fruit pulp of A. marmelos. Ripe fruit processing accounts for 60% of whole fruit mass while 40% remains unutilized and generates waste (hard shell, pomace, fiber and seeds) on a massive scale which do not have high value applications. A new flavone 3,5,7-trihydroxy-2-(4'-hydroxy-3'-isopentyloxyphenyl-4H-chromen-4-one (5) in addition to the known compounds 1-4, has been isolated from seeds of A. marmelos. Also, compound 7-(3'-methylbut-2'-enyloxy)-2H-chromen-2-one (2) has been isolated for the first time from A. marmelos. The structure of compounds 1-5 was determined by spectral analysis (UV, IR, NMR, etc.). Additionally, the non-edible oil obtained from seeds was investigated for waste to wealth recovery of 6-O-ascorbyl esters in high regioselectivity via one step semi-synthetic approach in the presence of ascorbic acid and H2SO4 at ambient temperature.


Assuntos
Aegle/química , Benzopiranos/isolamento & purificação , Flavonoides/isolamento & purificação , Benzopiranos/química , Ésteres/isolamento & purificação , Flavonoides/química , Extratos Vegetais/análise , Sementes/química
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