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1.
Virus Genes ; 56(1): 87-93, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-31696416

RESUMO

Even though alstroemeria mosaic virus (AlMV) is one of the most important viruses affecting alstroemeria plants, its genome is only partially available in public sequence databases. High throughput sequencing (HTS) of RNA from alstroemeria plants with symptoms of mosaic and streaking, collected in Lasso-Ecuador, indicated the presence of AlMV and lily symptomless virus. In this study, we aimed to assemble and characterize the complete genome sequence of AlMV. Reads from Illumina sequencing of ribosomal RNA-depleted total RNA were assembled into contigs that were mapped to the sunflower chlorotic mottle virus genome, revealing the 9774 [corrected] bp complete genome sequence of AlMV. Multiple sequence alignment of the AlMV polyprotein with close homologs allowed the identification of ten mature proteins P1, HC-Pro, P3, 6K1, CI, 6K2, NIa-VPg, NIa-Pro, NIb and CP. Furthermore, several potyvirus motifs were identified in the AlMV polyprotein including those related to potyvirus aphid transmission 334KMTC337, 592PTK594 and 2800DAG2802. Phylogenetic analysis based in the polyprotein showed that AlMV belongs to the potato virus Y clade and its closest relative is sunflower ring blotch virus. This study describes the first complete genome of AlMV and its placement within the genus Potyvirus, providing valuable information for future studies on this economically important virus.


Assuntos
Genoma Viral , Potyvirus/genética , Alstroemeria/virologia , Sequência de Bases , Filogenia , Doenças das Plantas/virologia , Potyvirus/classificação , Potyvirus/isolamento & purificação , Proteínas Virais/genética
2.
Arch Virol ; 164(1): 117-126, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30288607

RESUMO

An orthotospovirus distinct from all other orthotospoviruses was isolated from naturally infected alstroemeria plants. Disease symptoms caused by this virus mainly consisted of yellow spots on the leaves based on which the name alstroemeria yellow spot virus (AYSV) was coined. A host range analysis was performed and a polyclonal antiserum was produced against purified AYSV ribonucleoproteins which only reacted with the homologous antigen and not with any other (established or tentative) orthotospovirus from a selection of American and Asian species. Upon thrips transmission assays the virus was successfully transmitted by a population of Thrips tabaci. The entire nucleotide sequence of the M and S RNA segments was elucidated by a conventional cloning and sequencing strategy, and contained 4797 respectively 2734 nucleotides (nt). Simultaneously, a next generation sequencing (NGS) approach (RNAseq) was employed and generated contigs covering the entire viral tripartite RNA genome. In addition to the M and S RNA nucleotide sequences, the L RNA (8865 nt) was obtained. The nucleocapsid (N) gene encoded by the S RNA of this virus consisted of 819 nucleotides with a deduced N protein of 272 amino acids and by comparative sequence alignments to other established orthotospovirus species showed highest homology (69.5% identity) to the N protein of polygonum ringspot virus. The data altogether support the proposal of AYSV as a new orthotospovirus species within a growing clade of orthotospoviruses that seem to share the Middle East basin as a region of origin.


Assuntos
Alstroemeria/virologia , Doenças das Plantas/virologia , Vírus de Plantas/genética , Vírus de Plantas/isolamento & purificação , Animais , Insetos Vetores/virologia , Filogenia , RNA Viral/genética , RNA Viral/isolamento & purificação , Tisanópteros/virologia , Tabaco/virologia
3.
Biosci. j. (Online) ; 33(2): 321-332, mar./apr. 2017. tab
Artigo em Inglês | LILACS | ID: biblio-966129

RESUMO

Early leaf yellowing in cut alstroemeria (Alstroemeria aurantiaca) flowers before flower development and petal abscission is an important limiting postharvest quality and vase life factors. Early leaf senescence reduces postharvest longevity of cut flowers and promotes petal's wilting. A study was made to evaluate the response of cut alstroemeria flowers at varying concentrations of cycloheximide (CHI) (50, 100 and 200 mg l-1), coconut water (5, 10 and 20%) and 6-benzyladenine (BA) (50, 100 and 200 mg l-1). CHI, coconut water and BA extended the vase life at all concentrations compared to the control, but coconut water at 5% concentration (with 17.39 days) was the most effective treatment. Control cut flowers showed the least vase life (10.76 days). Ethylene production in cut flowers promoted flower senescence. All concentrations of CHI, coconut water and BA delayed ethylene production compared to the control. Treatment of cut flowers with coconut water at concentration of 5% maintained the highest fresh weight of flowers and increased the content of water uptake. The chlorophyll degradation was significantly reduced by the application of CHI, coconut water and BA. The maximum content of membrane's lipid peroxidation and antioxidant enzymes activity (super oxide dismutase and peroxidase) was obtained in control cut flowers. Thus, 5% fresh coconut water has the potential to be applied as vase solution (preservative medium) due to prolongs of cut alstroemeria flowers.


O amarelecimento precoce das folhas em flores de alstroemeria (Alstroemeria aurantiaca) cortadas antes do desenvolvimento floral e da abscisão de pétalas é um importante limitante da qualidade pós-colheita e dos fatores de vida do vaso. A senescência precoce da folha reduz a longevidade pós-colheita das flores cortadas e promove o murchamento da pétala. Um estudo foi realizado para avaliar a resposta de flores de alstroemeria cortadas em diferentes concentrações de cicloheximida (CHI) (50, 100 e 200 mg l-1), água de coco (5, 10 e 20%) e 6-benziladenina (BA) 50, 100 e 200 mg l-1). CHI, água de coco e BA prolongou a vida do vaso em todas as concentrações em comparação com o controle, mas a água de coco a 5% de concentração (com 17,39 dias) foi o tratamento mais eficaz. As flores cortadas de controlo mostraram a menor vida útil do vaso (10,76 dias). A produção de etileno em flores cortadas promoveu a senescência da flor. Todas as concentrações de CHI, água de coco e BA atrasaram a produção de etileno em comparação com o controle. O tratamento de flores cortadas com água de coco a uma concentração de 5% manteve o maior peso fresco de flores e aumentou o conteúdo de absorção de água. A degradação da clorofila foi significativamente reduzida pela aplicação de CHI, água de coco e BA. O teor máximo de atividade de enzimas antioxidantes e de peroxidação lipídica da membrana (super óxido dismutase e peroxidase) foi obtido em flores cortadas de controle. Assim, 5% de água de coco fresca tem potencial para ser aplicada como solução de vaso (meio de conservação) devido a prolongamentos das flores de alstroemeria cortadas.


Assuntos
Reguladores de Crescimento de Plantas , Cicloeximida , Alstroemeria , Alimentos de Coco
4.
PLoS One ; 8(6): e68180, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23950788

RESUMO

Monocots are one of the most diverse, successful and economically important clades of angiosperms. We attempt to analyse the complete plastid genome sequences of two lilies and their lengths were 152,793bp in Lilium longiflorum (Liliaceae) and 155,510bp in Alstroemeria aurea (Alstroemeriaceae). Phylogenetic analyses were performed for 28 taxa including major lineages of monocots using the sequences of 79 plastid genes for clarifying the phylogenetic relationship of the order Liliales. The sister relationship of Liliales and Asparagales-commelinids was improved with high resolution. Comparative analyses of inter-familial and inter-specific sequence variation were also carried out among three families of Liliaceae, Smilacaceae, and Alstroemeriaceae, and between two Lilium species of L. longflorum and L. superbum. Gene content and order were conserved in the order Liliales except infA loss in Smilax and Alstroemeria. IR boundaries were similar in IRa, however, IRb showed different extension patterns as JLB of Smilax and JSB in Alstroemeria. Ka/Ks ratio was high in matK among the pair-wise comparison of three families and the most variable genes were psaJ, ycf1, rpl32, rpl22, matK, and ccsA among the three families and rps15, rpoA, matK, and ndhF between Lilium.


Assuntos
Alstroemeria/genética , Genomas de Plastídeos , Lilium/genética , Sequência de Bases , Filogenia , Plastídeos/genética
5.
Am J Bot ; 99(9): 1501-12, 2012 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-22956470

RESUMO

PREMISE OF THE STUDY: Understanding the flexibility of monocot genomes requires a phylogenetic framework, which so far is available for few of the ca. 2800 genera. Here we use a molecular tree for the South American genus Alstroemeria to place karyological information, including fluorescent in situ hybridization (FISH) signals, in an explicit evolutionary context. METHODS: From a phylogeny based on plastid, nuclear, and mitochondrial sequences for most species of Alstroemeria, we selected early-branching (Chilean) and derived (Brazilian) species for which we obtained 18S-25S and 5S rDNA FISH signals; we also analyzed chromosome numbers, 1C-values, and telomere FISH signals (in two species). KEY RESULTS: Chromosome counts for Alstroemeria cf. rupestris and A. pulchella confirm 2n = 16 as typical of the genus, which now has chromosomes counted for 29 of its 78 species. The rDNA sites are polymorphic both among and within species, and interstitial telomeric sites in Alstroemeria cf. rupestris suggest chromosome fusion. CONCLUSIONS: In spite of a constant chromosome number, closely related species of Alstroemeria differ drastically in their rDNA, indicating rapid increase, decrease, or translocations of these genes. Previously proposed Brazilian and Chilean karyotype groups are not natural, and the n = 8 chromosomes in Alstroemeria compared to n = 9 in its sister genus Bomarea may result from a Robertsonian fusion.


Assuntos
Alstroemeria/genética , Cromossomos de Plantas/genética , DNA de Plantas/genética , DNA Ribossômico/genética , Evolução Molecular , Filogenia , Sequência de Bases , Hibridização in Situ Fluorescente , Cariotipagem , Funções Verossimilhança , Mitose/genética , Especificidade da Espécie , Telômero/genética
6.
Plant Sci ; 185-186: 298-308, 2012 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-22325893

RESUMO

Alstroemeria sp. cv. Green Coral has numerous bracts instead of flowers, and its cyme structures are repeated eternally. Observations of the development and morphology of inflorescence in cv. Green Coral revealed that transition from inflorescence to floral meristem was restricted. We isolated and characterized floral meristem identity genes LEAFY-like (AlsLFY) and SQUAMOSA-like (AlsSQa and AlsSQb) genes from Alstroemeria ligtu. In situ hybridization results indicated that AlsSQa and AlsSQb were expressed in the dome-shaped floral meristems and all floral organ primordia in A. ligtu. Transcripts of AlsLFY accumulated early in the dome-shaped floral meristems; the signals were restricted later to the outer region of the floral meristem. These results indicate that AlsLFY, AlsSQa, and AlsSQb function as floral meristem identity genes. Expression profiles of AlsLFY, AlsSQa, AlsSQb, and other MADS-box genes were compared between A. ligtu and cv. Green Coral. AlsLFY, AlsDEFa, and AlsAGL6 transcripts were not detected at the shoot apices of cv. Green Coral but were detected in A. ligtu. The early induction and accumulation of AlsLFY transcripts in the inflorescence meristem of A. ligtu prior to development of the floral meristem suggest that downregulation of AlsLFY is likely to restrict the inflorescence-to-floral meristem transition in cv. Green Coral.


Assuntos
Alstroemeria/genética , Regulação da Expressão Gênica no Desenvolvimento/genética , Inflorescência/genética , Meristema/genética , Proteínas de Plantas/genética , Alstroemeria/citologia , Alstroemeria/crescimento & desenvolvimento , Sequência de Bases , Regulação para Baixo/genética , Regulação da Expressão Gênica de Plantas/genética , Hibridização In Situ , Inflorescência/crescimento & desenvolvimento , Proteínas de Domínio MADS/genética , Proteínas de Domínio MADS/metabolismo , Meristema/crescimento & desenvolvimento , Dados de Sequência Molecular , Mutação , Fenótipo , Filogenia , Folhas de Planta/citologia , Folhas de Planta/genética , Folhas de Planta/crescimento & desenvolvimento , Proteínas de Plantas/metabolismo , Brotos de Planta/citologia , Brotos de Planta/genética , Brotos de Planta/crescimento & desenvolvimento , Análise de Sequência , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo
7.
J Exp Bot ; 63(7): 2739-52, 2012 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-22268153

RESUMO

Native to South America, Alstroemeria flowers are known for their colourful tepals, and Alstroemeria hybrids are an important cut flower. However, in common with many commercial cut flowers, virtually all the commercial Alstroemeria hybrids are not scented. The cultivar 'Sweet Laura' is one of very few scented commercial Alstroemeria hybrids. Characterization of the volatile emission profile of these cut flowers revealed three major terpene compounds: (E)-caryophyllene, humulene (also known as α-caryophyllene), an ocimene-like compound, and several minor peaks, one of which was identified as myrcene. The profile is completely different from that of the parental scented species A. caryophyllaea. Volatile emission peaked at anthesis in both scented genotypes, coincident in cv. 'Sweet Laura' with the maximal expression of a putative terpene synthase gene AlstroTPS. This gene was preferentially expressed in floral tissues of both cv. 'Sweet Laura' and A. caryophyllaea. Characterization of the AlstroTPS gene structure from cv. 'Sweet Laura' placed it as a member of the class III terpene synthases, and the predicted 567 amino acid sequence placed it into the subfamily TPS-b. The conserved sequences R(28)(R)X(8)W and D(321)DXXD are the putative Mg(2+)-binding sites, and in vitro assay of AlstroTPS expressed in Escherichia coli revealed that the encoded enzyme possesses myrcene synthase activity, consistent with a role for AlstroTPS in scent production in Alstroemeria cv. 'Sweet Laura' flowers.


Assuntos
Alquil e Aril Transferases/metabolismo , Alstroemeria/enzimologia , Flores/enzimologia , Liases Intramoleculares/metabolismo , Proteínas de Plantas/genética , Terpenos/metabolismo , Monoterpenos Acíclicos , Alcenos/química , Alcenos/metabolismo , Alquil e Aril Transferases/genética , Alstroemeria/classificação , Alstroemeria/genética , Alstroemeria/metabolismo , Sequência de Aminoácidos , Flores/genética , Flores/metabolismo , Regulação da Expressão Gênica de Plantas , Genótipo , Liases Intramoleculares/genética , Dados de Sequência Molecular , Monoterpenos/química , Monoterpenos/metabolismo , Filogenia , Proteínas de Plantas/metabolismo , Alinhamento de Sequência , Terpenos/química , Volatilização
8.
J Gen Virol ; 92(Pt 4): 988-96, 2011 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-21169212

RESUMO

In vegetatively propagated Alstroemeria plants that showed pronounced stunting and necrotic leaf spots, a tobravirus infection was diagnosed in which one tobacco rattle virus (TRV, strain AL) RNA1 species was associated with seven different RNA2 species. The latter differed considerably in size and in the types of their 3' RNA1-related sequences. The 5' RNA2-specific part of all these RNA2 molecules showed almost 100% sequence identity with that of RNA2 of the TRV isolate TCM from tulip, but in some of these RNA2 molecules it was shorter than in the TCM isolate, whereas in others it was longer. One of the TRV AL RNA2 molecules, i.e. TC3'PE-a, contained the full set of three full-length RNA2-specific ORFs (ORF2a, -2b and -2c), whereas the previously analysed TCM sequence contained only ORF2a and -2b. In four of these TRV AL RNA2 molecules, i.e. those that had a relatively short RNA2-specific part, the 3' end was identical to that of the cognate TRV AL RNA1, but in the other three, which had a long RNA2-specific part, it was closely related to that of pea early browning virus (PEBV) RNA1, which was not detected in the infected plants. A comparison with previously described TRV/PEBV RNA2 recombinants suggested that the various TRV AL RNA2 molecules may represent various steps and side steps in an evolutionary process, which is apt to open the wide host range of TRV also to PEBV-derived RNA2 species.


Assuntos
Alstroemeria/virologia , Vírus de Plantas/genética , Vírus de RNA/genética , Recombinação Genética , Deleção de Sequência , Tulipa/virologia , Evolução Molecular , Dados de Sequência Molecular , Fases de Leitura Aberta , Doenças das Plantas/virologia , Vírus de Plantas/classificação , Vírus de RNA/classificação , RNA Viral/genética , Análise de Sequência de DNA , Homologia de Sequência
9.
Rev. peru. biol. (Impr.) ; 17(3): 385-388, dic. 2010. tab
Artigo em Espanhol | LIPECS | ID: biblio-1111369

RESUMO

Se investigó el efecto antitumoral del extracto acuoso del bejuco Bomarea cornigera. Ratones de la cepa Swiss albina fueron inoculados con la línea tumoral TG-180 por 15 días; luego del cual se separaron en 5 grupos (n=5 por grupo). Se administro intraperitonealmente ciclofosfamida (control positivo), agua destilada (control negativo) y el extracto en concentraciones de 1X, 2X y 4X; se evaluó la morbilidad, mortalidad, el peso y la longitud del sarcoma. Se encontró un efecto inhibidor del extracto de B. cornigera en el desarrollo del tumor sólido en ratones en los cuales se les transplanto el sarcoma TG-180. Las tasas de inhibición fueron 87,44 y 8,52% después de 17 días de tratamiento considerando la dosis 1X (más baja) y 2X (intermedia), respectivamente. Estos resultados sugieren que la administración de extracto acuoso de B. cornigera vía intraperitoneal puede ser útil como inhibidor del cáncer.


Assuntos
Animais , Camundongos , Alstroemeria , Camundongos , Ensaios de Seleção de Medicamentos Antitumorais , Mikania/uso terapêutico , Plantas Medicinais
10.
J Exp Bot ; 61(11): 2905-21, 2010 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-20457576

RESUMO

Petal development and senescence entails a normally irreversible process. It starts with petal expansion and pigment production, and ends with nutrient remobilization and ultimately cell death. In many species this is accompanied by petal abscission. Post-harvest stress is an important factor in limiting petal longevity in cut flowers and accelerates some of the processes of senescence such as petal wilting and abscission. However, some of the effects of moderate stress in young flowers are reversible with appropriate treatments. Transcriptomic studies have shown that distinct gene sets are expressed during petal development and senescence. Despite this, the overlap in gene expression between developmental and stress-induced senescence in petals has not been fully investigated in any species. Here a custom-made cDNA microarray from Alstroemeria petals was used to investigate the overlap in gene expression between developmental changes (bud to first sign of senescence) and typical post-harvest stress treatments. Young flowers were stressed by cold or ambient temperatures without water followed by a recovery and rehydration period. Stressed flowers were still at the bud stage after stress treatments. Microarray analysis showed that ambient dehydration stress accelerates many of the changes in gene expression patterns that would normally occur during developmental senescence. However, a higher proportion of gene expression changes in response to cold stress were specific to this stimulus and not senescence related. The expression of 21 transcription factors was characterized, showing that overlapping sets of regulatory genes are activated during developmental senescence and by different stresses.


Assuntos
Alstroemeria/crescimento & desenvolvimento , Alstroemeria/fisiologia , Regulação da Expressão Gênica no Desenvolvimento , Água/metabolismo , Alstroemeria/genética , Temperatura Baixa , Flores , Regulação da Expressão Gênica de Plantas , Estresse Fisiológico
11.
Arch Virol ; 155(3): 423-8, 2010 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-20151164

RESUMO

A tospovirus causing necrotic streaks on leaves was isolated from Alstroemeria sp. in Colombia. Infected samples reacted positively with tomato spotted wilt virus (TSWV) antiserum during preliminary serological tests. Further analysis revealed a close serological relationship to tomato chlorotic spot virus (TCSV) and groundnut ringspot virus (GRSV). A major part of the S-RNA segment, encompassing the nucleocapsid (N) protein gene, the 5' untranslated region and a part of the intergenic region 3' of the N gene, was cloned and sequenced. The deduced N protein sequence showed highest amino acid identity (82%) to that of TCSV, indicating that the virus represents a new tospovirus species, for which the name Alstroemeria necrotic streak virus (ANSV) is coined. Phylogenetic analysis based on the N protein sequence revealed that this Alstroemeria-infecting tospovirus clustered with tospoviruses from the American continent. Frankliniella occidentalis was identified as potential vector species for ANSV.


Assuntos
Alstroemeria/virologia , Doenças das Plantas/virologia , Tospovirus/classificação , Tospovirus/genética , Clonagem Molecular , Análise por Conglomerados , Colômbia , Dados de Sequência Molecular , Filogenia , RNA Viral/genética , Análise de Sequência de DNA , Homologia de Sequência de Aminoácidos , Sorotipagem , Tospovirus/imunologia , Tospovirus/isolamento & purificação , Proteínas Virais/genética
12.
Plant Biol (Stuttg) ; 11(6): 878-85, 2009 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-19796365

RESUMO

The differentiation of a vegetative cell and a generative cell is a critical event during pollen development. The Lilium GlsA is known to localize in pollen and is considered to be involved in development of the generative cell. Here, we cloned a glsA ortholog from Alstroemeria, a commercially important cut flower. The expression of AaglsA (Alstroemeria aurea glsA) transcripts increased gradually after pollen mitosis I (PMI) and reached a significant level when the generative cell started to elongate. Analysis of the promoter of AaglsA suggests that AaglsA expression is controlled by several cis-regulatory elements during pollen development. This is the first investigation of reproductive factors regulating male gametogenesis in Alstroemeria.


Assuntos
Alstroemeria/genética , Proteínas de Plantas/genética , Regiões Promotoras Genéticas , Alstroemeria/crescimento & desenvolvimento , Alstroemeria/metabolismo , Sequência de Bases , Clonagem Molecular , Regulação da Expressão Gênica no Desenvolvimento , Regulação da Expressão Gênica de Plantas , Mitose , Dados de Sequência Molecular , Proteínas de Plantas/metabolismo , Pólen/metabolismo , RNA Mensageiro/metabolismo
13.
J Plant Res ; 122(2): 225-34, 2009 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-19151916

RESUMO

This study aimed to analyze male gamete behavior from mature pollen to pollen tube growth in the bicellular pollen species Alstroemeria aurea. For mature pollen, pollen protoplasts were examined using flow cytometry. The protoplasts showed two peaks of DNA content at 1C and 1.90C. Flow cytometry at different developmental stages of pollen tubes cultured in vitro revealed changes in the nuclear phase at 9 and 18 h after culture. Sperm cell formation occurred at 6-9 h after culture, indicating that the first change was due to the division of the generative cells into sperm cells. After sperm cell formation, the number of vegetative nucleus associations with sperm cells showed a tendency to increase. This association was suggested as the male germ unit (MGU). When sperm cells, vegetative nuclei, and partial MGUs were collected separately from pollen tubes cultured for 18 h and analyzed using a flow cytometer, the sperm cells and vegetative nuclei contained 1C DNA, while the DNA content of partial MGUs was counted as 2C. Therefore, the second change in the nuclear phase, which results in an increase in 2C nuclei, is possibly related to the formation of MGUs.


Assuntos
Alstroemeria/genética , Núcleo Celular/metabolismo , Citometria de Fluxo/métodos , Tubo Polínico/crescimento & desenvolvimento , Pólen/citologia , Pólen/metabolismo , Diferenciação Celular , DNA de Plantas/química , Genes de Plantas , Tubo Polínico/genética
14.
Plant Cell Physiol ; 48(2): 310-21, 2007 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-17205968

RESUMO

Alstroemeria (Liliales) has two layers of petaloid tepals, in which the often spotted narrow inner tepals can be distinguished easily from the wider outer tepals. In order to explore this floral morphology in Alstroemeria, we investigated the tepal morphology and the expression patterns of three class B genes, whose homologs in eudicots have been shown previously to be involved in petal and stamen development. The two DEF-like genes (AlsDEFa and AlsDEFb) and the one GLO-like gene (AlsGLO) of Alstroemeria ligtu were isolated by rapid amplification of cDNA ends (RACE). Northern hybridization, reverse transcription-PCR (RT-PCR) and in situ hybridization analyses indicated that AlsDEFb and AlsGLO were expressed in whorls 1, 2 and 3 (outer tepals, inner tepals and stamens, respectively), whereas AlsDEFa expression was detected only in whorls 2 and 3. These results suggest that in A. ligtu, AlsDEFb and AlsGLO would participate in determining the organ identity of the two-layered petaloid tepals and stamens, which is in support of the modified ABC model. Additionally, the distinctive expression patterns of AlsDEFa and AlsDEFb might be related to morphological differences between the two-layered tepals.


Assuntos
Alstroemeria/genética , Regulação da Expressão Gênica de Plantas , Genes de Plantas , Northern Blotting , Flores , Hibridização In Situ , Dados de Sequência Molecular , Reação em Cadeia da Polimerase Via Transcriptase Reversa
15.
Ann Bot ; 97(6): 1139-44, 2006 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-16621859

RESUMO

AIMS: To develop a procedure for isolating living egg cells and zygotes from Alstroemeria ovules. SCOPE: An attempt was made to isolate egg cells and zygotes from the ovules of Alstroemeria aurea. The ovules were histologically observed using a clearing procedure which revealed the localization and sizes of the embryo sacs and egg apparatus within the ovules. For the isolation of egg cells, ovules were cut into sections with a surgical blade and treated with an enzyme solution. Subsequently, these ovule sections were dissected using a glass needle under an inverted microscope. Egg cells successfully isolated by this procedure were collected using microcapillaries connected to a micropump. For zygote isolation, ovules were excised from ovaries 24 h after self-pollination. By treating excised ovules with an enzyme solution and subsequently dissecting them using a glass needle, zygotes were successfully isolated from the ovules and collected with a microcapillary. The isolated zygotes were associated with pollen tubes and one of the synergids. Egg cells and zygotes were viable for up to 2 h following isolation, as determined by fluorescein diacetate staining. CONCLUSIONS: The procedures for isolating egg cells and zygotes in Alstroemeria were established, and each egg cell and zygote was captured with a microcapillary.


Assuntos
Alstroemeria/citologia , Flores/citologia , Microdissecção/métodos , Óvulo , Fertilização/fisiologia , Pólen/fisiologia
16.
Allergol Immunopathol (Madr) ; 34(2): 73-5, 2006.
Artigo em Inglês | MEDLINE | ID: mdl-16606550

RESUMO

BACKGROUND: Occupational allergy among florists and people who work in cut flower production of Alstroemeria cultivars (Peruvian lily or Inca lily) has been previously reported. The allergen involved in sensitization is tulipalin A (alpha-methylene-gamma-butyrolactone). CASE REPORT: We describe the case of a 65-year-old woman who developed severe dermatitis on her right thumb, index and middle fingers and less severe dermatitis on her left palm and front of forearm with occasional itching of the neck and face after taking up home gardening activities, including cutting flowers such as Alstroemeria. METHODS: The patient and three healthy individuals were submitted to epicutaneous tests with the European standard series, the plant series, and stem portions of three suspected ornamental plants (Alstroemeria, Lilium and Zantedeschia), garlic, and onion. RESULTS: Patch tests performed in our patient, revealed an extreme reaction (+ + +) to Alstroemeria and alpha-methylene-gamma-butyrolactone, a strong reaction (+ +) to propolis and wood tar mix, a weak reaction (+) to balsam of Peru, an irritant reaction to garlic and negative results to diallyl disulfide and the other components investigated. Patch tests performed in the healthy individuals revealed negative. CONCLUSIONS: We stress the importance of Alstroemeria as a cause of allergic contact dermatitis not only in workers involved in the flower trade, but also in other people that come into contact with this plant in their leisure activities.


Assuntos
Alstroemeria/efeitos adversos , Dermatite Alérgica de Contato/etiologia , Jardinagem , Dermatoses da Mão/etiologia , Idoso , Feminino , Dedos , Humanos , Fatores de Risco
17.
Allergol. immunopatol ; 34(2): 73-75, mar. 2006. ilus
Artigo em En | IBECS | ID: ibc-046878

RESUMO

Background: Occupational allergy among florists and people who work in cut flower production of Alstroemeria cultivars (Peruvian lily or Inca lily) has been previously reported. The allergen involved in sensitization is tulipalin A (alpha-methylene-gamma-butyrolactone). Case report: We describe the case of a 65-year-old woman who developed severe dermatitis on her right thumb, index and middle fingers and less severe dermatitis on her left palm and front of forearm with occasional itching of the neck and face after taking up home gardening activities, including cutting flowers such as Alstroemeria. Methods: The patient and three healthy individuals were submitted to epicutaneous tests with the European standard series, the plant series, and stem portions of three suspected ornamental plants (Alstroemeria, Lilium and Zantedeschia), garlic, and onion. Results: Patch tests performed in our patient, revealed an extreme reaction (+ + +) to Alstroemeria and alpha-methylene-gamma-butyrolactone, a strong reaction (+ +) to propolis and wood tar mix, a weak reaction (+) to balsam of Peru, an irritant reaction to garlic and negative results to diallyl disulfide and the other components investigated. Patch tests performed in the healthy individuals revealed negative. Conclusions: We stress the importance of Alstroemeria as a cause of allergic contact dermatitis not only in workers involved in the flower trade, but also in other people that come into contact with this plant in their leisure activities


Antecedentes: La alergia ocupacional entre las floristas y en los trabajadores de producción de flores de corte de cultivos de Alstroemeria (lirio Peruano o lirio Inca) fue ya objeto de descripción. La Tulipalina A (alfa-metileno-gama-butirolactona) es lo alergeno involucrado en la sensibilización. Caso clínico: se presenta un caso de una paciente de 65 años que ha iniciado una dermatitis grave en los 1º, 2º y 3º dedos de la mano derecha, bien como una dermatitis de intensidad moderada en la área palmar izquierda y vista anterior de lo antebrazo homolateral. Ocasionalmente, los síntomas se acompañan de prurito de la cara y cuello, en el decurso de actividades de jardinaje en su casa, incluyendo el corte de flores de Alstroemeria. Métodos: Fueron realizadas pruebas epicutáneas con la serie estándar europea, la serie de plantas, y porciones de tallo de tres plantas sospechas (Alstroemeria, Lilium y Zantedeschia), ajo y cebolla. Resultados: Las pruebas revelaron una reacción severa (+++) a Alstroemeria y alfa-metileno-gama-butirolactona, una reacción intensa (++) a propolis y mixtura de alquitranes de la madera, una reacción ligera (+) a bálsamo Perú, una reacción irritativa a ajo y resultados negativos a dialil disulfido, bien como a todos los demás estudiados. Conclusiones: Este caso clínico reporta la importancia de la Alstroemeria como inductor de dermatitis de contacto alérgica, non solo en trabajadores de floricultura, pero también en individuos comunes que puedan contactar con esta planta en sus actividades de tiempos libres


Assuntos
Feminino , Idoso , Humanos , Alstroemeria/efeitos adversos , Dermatoses da Mão/etiologia , Dermatite Alérgica de Contato/etiologia , Dedos , Fatores de Risco
18.
Arch Virol ; 150(11): 2377-85, 2005 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-15986173

RESUMO

A flexuous virus was isolated in Japan from an alstroemeria plant showing mosaic symptoms. The virus had a broad host range but had systemically latent infectivity in alstroemeria. The virus was assigned to the genus Potexvirus based on morphology and physical properties and on an analysis of the complete nucleotide sequence. The genomic RNA of the virus was 7,009 nucleotides in length, excluding the 3'-terminal poly (A) tail. It contained five open reading frames (ORFs), which was consistent with other members of the genus Potexvirus. Although nucleotide sequences of the ORFs differ from previously reported potexviruses, a phylogenetic analysis placed it phylogenetically close to Narcissus mosaic virus and Scallion virus X. Therefore, we propose that this virus should be designated as Alstroemeria virus X (AlsVX).


Assuntos
Alstroemeria/virologia , Potexvirus/genética , Sequência de Bases , Sequência Consenso , Primers do DNA , Potexvirus/classificação , Potexvirus/isolamento & purificação
19.
J Exp Bot ; 56(413): 1007-16, 2005 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-15689338

RESUMO

Senescence of floral organs is broadly divided into two groups: those that exhibit sensitivity to exogenous ethylene and those that do not. Endogenous ethylene production from the former group is via a well-characterized biochemical pathway and is either due to developmental or pollination-induced senescence. Many flowers from the order Liliales are characterized as ethylene-insensitive since they do not appear to produce endogenous ethylene, or respond to exogenous ethylene treatments, however, the majority of cases studied are wilting flowers, rather than those where life is terminated by perianth abscission. The role of ethylene in the senescence and abscission of Alstroemeria peruviana cv. Rebecca and cv. Samora tepals was previously unclear, with silver treatments recommended for delaying leaf rather than flower senescence. In the present paper the effects of exogenous ethylene, 2-chloroethylphosphonic acid (CEPA) and silver thiosulphate (STS) treatments on tepal senescence and abscission have been investigated. Results indicate that sensitivity to ethylene develops several days after flower opening such that STS only has a limited ability to delay tepal abscission. Detachment force measurements indicate that cell separation events are initiated after anthesis. Endogenous ethylene production was measured using laser photoacoustics and showed that Alstroemeria senesce independently of ethylene production, but that an extremely small amount of ethylene (0.15 nl flower(-1) h(-1)) is produced immediately prior to abscission. Investigation of the expression of genes involved in ethylene biosysnthesis by semi-quantitative RT-PCR indicated that transcriptional regulation is likely to be at the level of ACC oxidase, and that the timing of ACC oxidase gene expression is coincident with development of sensitivity to exogenous ethylene.


Assuntos
Alstroemeria/enzimologia , Etilenos/biossíntese , Flores/enzimologia , Alstroemeria/efeitos dos fármacos , Alstroemeria/crescimento & desenvolvimento , Aminoácido Oxirredutases/metabolismo , Perfilação da Expressão Gênica , Regulação da Expressão Gênica no Desenvolvimento/fisiologia , Regulação Enzimológica da Expressão Gênica/fisiologia , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Liases/metabolismo , Compostos Organofosforados/farmacologia , Reguladores de Crescimento de Plantas/biossíntese , Reguladores de Crescimento de Plantas/fisiologia , Tiossulfatos/farmacologia , Fatores de Tempo
20.
Arnaldoa ; 11(2): 21-27, dic. 2004. ilus
Artigo em Inglês | LIPECS | ID: biblio-1106325

RESUMO

Se describe e ilustra una nueva especie de Bomarea subgénero Bomarea s.str. (Alstroemeriaceae) procedente de la región Andina del Norte del Perú (departamentos de Amazonas, La Libertad y San Martín): B. alstroemeroides Hofreiter & E. Rodr. Se discute la forma típica del crecimiento y su variabilidad, así como las preferencias del hábitat y distribución. La nueva especie se reconoce fácilmente por sus flores grandes, muy abiertas y claramente zigomorfas. La forma de la flor es similar a ciertas especies de Alstroemeria. El nuevo taxón es endémico a la región de Amotape-Huancabamba. Se compara su relación con las especies mas afines.


A new species of Bomarea sbgenus Bomarea s.str. (Alstroemeriaceae), from Northern Peruvian Andean region (Amazonas, La Libertad and San Martin departments) is described and illustrated: B. alstroemeroides Hofreiter & E. Rodr. The typical growth form and its variability, habitat preferences and distribution are discussed. The new species is easily recognized because of its large wide-open, clearly zygomorphic flowers. The shape of the flower appears similar to some Alstroemeria species. The species is endemic to the Amotape-Huancabamba-region. The new species is compared to its next relatives.


Assuntos
Alstroemeria , Ecossistema Andino , Peru
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