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1.
Anaerobe ; 69: 102327, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33515721

RESUMO

Anaerobiospirillum succiniciproducens is a gram-negative, spiral-shaped anaerobe, that is a rare but potentially lethal cause of bacteremia in humans, particularly in immunocompromised hosts. We reported a 69-year-old HIV-infected male presenting with dysphagia, odynophagia and fulminant pneumonia who died. In addition, in a literature review, we summarized the characteristics of 19 adult patients with A. succiniciproducens bacteremia, which were confirmed by matrix-assisted laser desorption ionization-time of flight mass spectrometry or molecular methods. Among those, the presentation of gastrointestinal conditions was the only independent risk factor for mortality. Clinicians should be aware of this pathogen, especially when a culture is negative but a Gram stain reveals gram-negative spiral-shaped bacteria.


Assuntos
Anaerobiospirillum/genética , Anaerobiospirillum/isolamento & purificação , Anaerobiospirillum/patogenicidade , Infecções por Bactérias Gram-Negativas/etiologia , Infecções por HIV/sangue , Infecções por HIV/complicações , Infecções por HIV/microbiologia , Idoso , Evolução Fatal , Infecções por Bactérias Gram-Negativas/genética , Infecções por Bactérias Gram-Negativas/mortalidade , Infecções por HIV/mortalidade , Humanos , Masculino , Taiwan
3.
BMC Musculoskelet Disord ; 20(1): 301, 2019 Jun 26.
Artigo em Inglês | MEDLINE | ID: mdl-31238924

RESUMO

BACKGROUND: We report a case of prosthetic hip joint infection in a heart transplant recipient due to Anaerobiospirillum succiniciproducens, a genus of spiral-shaped curved anaerobic gram-negative rod which colonizes the gastrointestinal tract of cats and dogs. Invasive infections in humans are rare and typically occur in immunocompromised hosts. CASE PRESENTATION: A 65-year-old male dog breeder with a history of rheumatoid arthritis, bilateral hip arthroplasties, and non-ischemic cardiomyopathy with a heart transplant 10 years ago presented with a three month history of progressive left hip pain and frank purulence on hip aspiration. He underwent irrigation and debridement of the left hip and one-stage revision with hardware exchange. Although gram stain and culture from synovial fluid and intraoperative cultures were initially negative, anaerobic cultures from tissue specimens later grew a spiral-shaped gram-negative rod, identified as Anaerobiospirillum spp. by 16S rRNA gene sequencing. The patient was treated with ceftriaxone 2 g daily for 6 weeks with a good response to treatment. A similar organism was unable to be isolated from culture of 2 of the patient's dogs, however, they were thought to be the most likely source of his infection. CONCLUSION: Anaerobiospirillum spp. should be considered in immunocompromised patients with exposure to dogs or cats who present with bacteremia, gastrointestinal infection, pyomyositis, or prosthetic joint infections, especially in cases of culture-negative or with anaerobic culture growth.


Assuntos
Anaerobiospirillum/isolamento & purificação , Microbioma Gastrointestinal , Infecções por Bactérias Gram-Negativas/microbiologia , Hospedeiro Imunocomprometido , Infecções Relacionadas à Prótese/microbiologia , Idoso , Anaerobiospirillum/imunologia , Animais , Cães , Rejeição de Enxerto/imunologia , Rejeição de Enxerto/prevenção & controle , Infecções por Bactérias Gram-Negativas/imunologia , Infecções por Bactérias Gram-Negativas/transmissão , Transplante de Coração/efeitos adversos , Humanos , Imunossupressores/efeitos adversos , Masculino , Infecções Relacionadas à Prótese/imunologia , Infecções Relacionadas à Prótese/transmissão
4.
Anaerobe ; 55: 130-135, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30557657

RESUMO

Anaerobiospirillum succiniciproducens is a gram-negative anaerobic spiral rod which is part of the normal flora of dogs and cats and can produce bacteraemia and diarrhoea in humans. In this report we describe two cases of bacteraemia caused by A. succiniciproducens which was successfully identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). We present a comprehensive literature review of 48 cases of A. succiniciproducens bacteraemia in which we describe previous underlying conditions, clinical presentations, identification methodology and antibiotic susceptibility data.


Assuntos
Anaerobiospirillum/isolamento & purificação , Bacteriemia/diagnóstico , Bacteriemia/microbiologia , Técnicas Bacteriológicas/métodos , Infecções por Bactérias Gram-Negativas/diagnóstico , Infecções por Bactérias Gram-Negativas/microbiologia , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos , Adulto , Idoso , Idoso de 80 Anos ou mais , Anaerobiospirillum/química , Anaerobiospirillum/classificação , Diarreia/diagnóstico , Diarreia/microbiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade
6.
Anaerobe ; 54: 267-270, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29626623

RESUMO

Anaerobiospirillum succiniciproducens is known as an uncommon cause of diarrhea and bacteremia in humans, usually in immunocompromised hosts. We report four cases of A. succiniciproducens bloodstream infection in different hosts, including a previously healthy man. We describe clinical features, antibiotics susceptibility profile, treatment and outcomes. Strains were identified by 16S rRNA gene sequences which contributed to the extension of our MALDI-TOF MS database.


Assuntos
Anaerobiospirillum/isolamento & purificação , Bacteriemia/microbiologia , Infecções por Bactérias Gram-Negativas/microbiologia , Adulto , Idoso de 80 Anos ou mais , Anaerobiospirillum/química , Anaerobiospirillum/efeitos dos fármacos , Anaerobiospirillum/genética , Antibacterianos/uso terapêutico , Bacteriemia/diagnóstico , Bacteriemia/tratamento farmacológico , DNA Bacteriano/genética , Feminino , Infecções por Bactérias Gram-Negativas/diagnóstico , Infecções por Bactérias Gram-Negativas/tratamento farmacológico , Humanos , Masculino , Pessoa de Meia-Idade , RNA Ribossômico 16S/genética , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos
7.
Neth J Med ; 76(2): 87-89, 2018 03.
Artigo em Inglês | MEDLINE | ID: mdl-29515010

RESUMO

A 40-year-old woman with a history of liver cirrhosis presented with septic shock caused by an Anaerobiospirillum succiniciproducens bacteraemia after a cat bite. A. succiniciproducens sepsis can develop after a cat or dog bite, especially in immunocompromised hosts, or might occur after translocation from the gut flora. It is a potentially lethal infection.


Assuntos
Anaerobiospirillum/isolamento & purificação , Antibacterianos/uso terapêutico , Mordeduras e Picadas/complicações , Gatos , Infecções por Bactérias Gram-Negativas/complicações , Choque Séptico/microbiologia , Adulto , Animais , Feminino , Infecções por Bactérias Gram-Negativas/tratamento farmacológico , Infecções por Bactérias Gram-Negativas/microbiologia , Humanos , Choque Séptico/tratamento farmacológico
9.
Rev Argent Microbiol ; 49(2): 146-152, 2017.
Artigo em Espanhol | MEDLINE | ID: mdl-28506633

RESUMO

Two cases of insidious bacteremia by uncommon curve and spiral-shaped, motile anaerobic gram-negative rods are presented. Both of them were of an unclear origin and occurred in immunosuppressed patients with simultaneous diseases. The key tests for the identification of Anaerobiospirillum were its micromorphology, a strictly anaerobic condition, negative catalase activity, the special-potency disk profile, glucose fermentation, and ß-NAG production. Desulfovibrio species was identified by all the above preliminary tests but with a different disk profile, as well as for being asaccharolytic and desulfoviridin and H2S producer. We here alert about the resistance or intermediate susceptibility of Anaerobiospirillum succiniciproducens against antimicrobial agents, such as metronidazole, one of the first-line drugs used for the treatment of anaerobic gram-negative infections. Aminopenicillins with ß-lactamase-inhibitor combinations and imipenem were active for this agent. Desulfovibrio desulfuricans was ß-lactamase producer and resistant to cephalosporins, while metronidazole, imipenem and levofloxacin were active. A reliable identification of these microorganisms is important for establishing the best therapeutic scheme.


Assuntos
Anaerobiospirillum , Bacteriemia/microbiologia , Desulfovibrio desulfuricans , Infecções por Bactérias Gram-Negativas , Anaerobiospirillum/isolamento & purificação , Antibacterianos , Desulfovibrio desulfuricans/isolamento & purificação , Infecções por Bactérias Gram-Negativas/diagnóstico , Infecções por Bactérias Gram-Negativas/tratamento farmacológico , Humanos , Hospedeiro Imunocomprometido
12.
Anaerobe ; 39: 28-30, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-26899447

RESUMO

Anaerobiospirillum succiniciproducens is a rare but potentially lethal pathogen. We report a case of A. succiniciproducens bloodstream infection in a 55-year-old man hospitalized for pelvic trauma. The strain was identified by 16sRNA sequencing after several failures of identification by MALDI-TOF MS. The strain was susceptible to beta-lactam antibiotics and ciprofloxacin, but resistant to macrolides and clindamycin. Identification tools must be improved to enhance our knowledge on this rare pathogen and to define optimal therapy.


Assuntos
Anaerobiospirillum/isolamento & purificação , Infecções por Bactérias Gram-Negativas/diagnóstico , Infecções por Bactérias Gram-Negativas/microbiologia , RNA Ribossômico 16S/genética , Anaerobiospirillum/classificação , Anaerobiospirillum/genética , Antibacterianos/uso terapêutico , Ciprofloxacina/uso terapêutico , Clindamicina/uso terapêutico , Diagnóstico Tardio , Farmacorresistência Bacteriana , Infecções por Bactérias Gram-Negativas/tratamento farmacológico , Infecções por Bactérias Gram-Negativas/patologia , Humanos , Masculino , Testes de Sensibilidade Microbiana , Pessoa de Meia-Idade , Pelve/lesões , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , beta-Lactamas/uso terapêutico
13.
Rev Argent Microbiol ; 47(4): 328-30, 2015.
Artigo em Espanhol | MEDLINE | ID: mdl-26654699

RESUMO

Anaerobiospirillum thomasii has been reported as a causative agent of diarrhea in humans; however no bacteremia associated with this pathogen has been described so far. We present here the first case of fatal A. thomasii bacteremia in an alcoholic patient.


Assuntos
Anaerobiospirillum , Bacteriemia , Infecções por Bactérias Gram-Negativas , Bacteriemia/diagnóstico , Evolução Fatal , Feminino , Infecções por Bactérias Gram-Negativas/diagnóstico , Humanos , Pessoa de Meia-Idade
14.
Rev. argent. microbiol ; 47(4): 328-330, dic. 2015.
Artigo em Espanhol | LILACS | ID: biblio-1141103

RESUMO

Anaerobiospirillum thomasii ha sido descrito como causante de diarrea en humanos, pero no se han informado bacteriemias asociadas a este organismo. En esta comunicación describimos el primer aislamiento de A. thomasii como causa de bacteriemia fatal en una paciente alcohólica


Anaerobiospirillum thomasii has been reported as a causative agent of diarrhea in humans; however no bacteremia associated with this pathogen has been described so far. We present here the first case of fatal A. thomasii bacteremia in an alcoholic patient


Assuntos
Humanos , Feminino , Pessoa de Meia-Idade , Bacteriemia/complicações , Anaerobiospirillum/patogenicidade , RNA Ribossômico 16S/análise , Bacteriemia/microbiologia , Evolução Fatal , Anaerobiospirillum/metabolismo
15.
J Biotechnol ; 193: 130-3, 2015 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-25483318

RESUMO

The dynamics of the membrane fatty acid composition of a succinic acid-producing bacterium, Anaerobiospirillum succiniciproducens, was studied using continuous and batch cultures. In both batch and chemostat continuous culture conditions, A. succiniciproducens grown on glucose or glycerol responded to acid stress by lowering the content of saturated fatty acids (SFAs) and increasing the content of unsaturated fatty acids (UFAs). Notably, A. succiniciproducens elongated C16:0 (SFA) and converted it to C18:1 (UFA) in the pH-uncontrolled culture, which is a common bacterial adaptation strategy to combat acid stress. Interestingly, A. succiniciproducens did not exhibit a significant increase in cyclic FA content, which is another common strategy to cope with acidic conditions.


Assuntos
Anaerobiospirillum/metabolismo , Membrana Celular/química , Ácidos Graxos/análise , Ácido Succínico/metabolismo , Técnicas de Cultura Celular por Lotes , Reatores Biológicos , Meios de Cultura , Ácidos Graxos/química , Ácidos Graxos/metabolismo , Glucose/metabolismo , Glicerol/metabolismo , Ácido Succínico/análise
16.
Am J Emerg Med ; 32(7): 812.e1-3, 2014 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-24440590

RESUMO

Anaerobiospirillum succiniciproducens is rarely associated with bacteremia but results in significant mortality. Almost all reported bacteremia cases have occurred in immunocompromised hosts, such as those with alcoholic liver disease, atherosclerosis, recent surgery, malignancies, or acquired immunodeficiency syndrome. We describe here, to our knowledge, the first clinical evidence for A succiniciproducens bacteremia in a healthy man. A 61-year-old man had fallen from a roof and was admitted to our emergency department with severe left flank pain without an external wound. He was given transcatheter arterial embolization for the left kidney injury on the same day, and his condition stabilized. Four days after admission, he had fever without gastrointestinal signs and symptoms. Spiral-shaped, gram-negative anaerobic bacteria were isolated from 2 sets of blood cultures, and the oxidase and catalase test results were negative. The isolated bacteria were different from the Campylobacter spp. On the next day, the bacteria were confirmed as A succiniciproducens by 16S rRNA sequencing. The patient responded to sulbactam/ampicillin. On day 13, the patient was discharged with a 7-day prescription for oral amoxicillin/clavulanate. Six months after admission, the patient was free of recurrent infection. A succiniciproducens bacteremia can occur in healthy adults. When large gram-negative spiral-shaped bacteria are detected, this bacterial species should be considered and differentiated from the Campylobacter spp because A succiniciproducens is often resistant to macrolide antibiotics.


Assuntos
Anaerobiospirillum/genética , Bacteriemia/microbiologia , Infecções por Bactérias Gram-Negativas/microbiologia , Imunocompetência , RNA Bacteriano/análise , RNA Ribossômico 16S/análise , Ampicilina/uso terapêutico , Antibacterianos/uso terapêutico , Bacteriemia/diagnóstico , Bacteriemia/tratamento farmacológico , Infecções por Bactérias Gram-Negativas/diagnóstico , Infecções por Bactérias Gram-Negativas/tratamento farmacológico , Humanos , Masculino , Pessoa de Meia-Idade , Sulbactam/uso terapêutico
17.
Sheng Wu Gong Cheng Xue Bao ; 29(10): 1386-97, 2013 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-24432654

RESUMO

Succinic acid is one of the key intermediates in the tricarboxylic acid cycle (TCA)and has huge potentials in biopolymer, food, medicine applications. This article reviews recent research progress in the production of succinic acid by microbial fermentation, including discovery and screening of the succinic-acid-producing microbes, the progress of genetic engineering strategy and metabolic engineering technology for construction of succinic acid-producing strains, and fermentation process control and optimization. Finally, we discussed the limitation of current progress and proposed the future research needs for microbial production of succinic acid.


Assuntos
Actinobacillus/metabolismo , Anaerobiospirillum/metabolismo , Microbiologia Industrial/métodos , Engenharia Metabólica/métodos , Ácido Succínico/metabolismo , Actinobacillus/genética , Anaerobiospirillum/genética , Fermentação , Microbiologia Industrial/tendências
18.
Biotechnol Bioeng ; 109(5): 1187-92, 2012 May.
Artigo em Inglês | MEDLINE | ID: mdl-22125258

RESUMO

The aim of this work was the development of a feed-control for a succinic acid production fed-batch process. The performed batch trials indicated a correlation between succinic acid production and base consumption pH control. Based on the metabolism of Anaerobiospirillum succiniciproducens, a theoretical correlation between base consumption and glucose feed was established and proved in cultivation trials. With the established fed-batch process, the succinic acid yield could be increased to 0.875 (g/g glucose) in comparison to batch processes (0.60) with similar glucose concentrations. Additionally, the results indicate that the osmolarity of the medium has a significant influence on succinic acid production.


Assuntos
Anaerobiospirillum/crescimento & desenvolvimento , Anaerobiospirillum/metabolismo , Meios de Cultura/química , Ácido Succínico/metabolismo , Glucose/metabolismo , Concentração de Íons de Hidrogênio , Modelos Teóricos
20.
South Med J ; 104(3): 205-14, 2011 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-21297548

RESUMO

Anaerobiospirillum succiniciproducens (A succiniciproducens), a spiral, Gram-negative anaerobic rod which is part of the normal intestinal flora of cats and dogs, has rarely been reported as a cause of bacteremia and diarrhea in humans, particularly in immunocompromised hosts. Although it can be associated with significant mortality, the full extent of its pathogenicity, clinical spectrum, and optimal therapy remain to be determined. We review the available literature on microbiology, clinical manifestations, and treatment options for Anaerobiospirillum infection.


Assuntos
Anaerobiospirillum/patogenicidade , Bacteriemia/diagnóstico , Bacteriemia/terapia , Infecções por Bactérias Gram-Negativas/diagnóstico , Infecções por Bactérias Gram-Negativas/terapia , Bacteriemia/microbiologia , Infecções por Bactérias Gram-Negativas/microbiologia , Humanos
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