Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 123
Filtrar
1.
Methods Mol Biol ; 2288: 103-111, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34270007

RESUMO

Doubled haploidy technology is a powerful tool to accelerate the breeding of new crop varieties. Protocols are not universal, as even species within the same family require a specific process. Here we describe methods for developing doubled haploids for fennel and dill, both Apiaceae species which are used for food, flavorings, and medicine.


Assuntos
Anethum graveolens/crescimento & desenvolvimento , Anethum graveolens/genética , Foeniculum/crescimento & desenvolvimento , Foeniculum/genética , Melhoramento Vegetal/métodos , Produtos Agrícolas/genética , Produtos Agrícolas/crescimento & desenvolvimento , Meios de Cultura/química , Diploide , Haploidia , Homozigoto , Biologia Molecular/métodos , Pólen/genética , Pólen/crescimento & desenvolvimento , Técnicas de Cultura de Tecidos
2.
Sci Rep ; 11(1): 10390, 2021 05 17.
Artigo em Inglês | MEDLINE | ID: mdl-34001970

RESUMO

Mycological investigations of 25 samples of stored chickpea food seeds (Cicer arietinum L.) from grocery stores of Gurgaon and Gorakhpur revealed occurrence of seventeen fungal species belonging to genus viz., Alternaria, Aspergillus, Chaetomium, Colletotrichum, Curvularia, Fusarium, Penicillium, Rhizopus, Rhizoctonia, and Sclerotium. In these Aspergillus flavus, A. niger, Fusarium oxysporum had dominance in terms of per cent occurrence. Only one species of Bruchid (Callosobruchus chinensis L.) occurred in all the 25 samples. The biodeterioration of seeds inoculated with fungi: A. flavus, A. niger, F. oxysporum and the insect-C. chinensis, revealed their role in seed deterioration. For chickpea food seed protection essential oils were extracted from edible commodity(clove(Lavang and dill(sowa) leaf). Clove(Lavang) oil registered highest antifungal activity inhibiting (100%) mycelial growth of fungi, viz. species Aspergillus flavus, A. niger, Fusarium oxysporum at 300 ppm but was fungicidal at 400 ppm. Dill (Sowa) oil showed complete inhibition at 400 ppm and was fungicidal at 500 ppm. While mixture of both the oils (clove and dill) showed complete inhibition (100%) and fungicidal action at 400 ppm against the dominant fungi. The oils showed 100% insect repellent activity and were found fungicidal at 0.02 ml dose and also insecticidal. The mixture of oils was cidal at 0.02 ml dose. The mixture of oils showed a broad antifungal spectrum at 500 ppm while only 70-93% inhibitory activity at 300 ppm. The oils' mixture's activity was not affected by temp, storage and autoclaving up to 150 days. Oils physico-chemical properties were studied. GC-MS analysis of clove(Lavang) oil depicted major components: 75.63%eugenol while dill(sowa) leaf oil had 25.14% apiole. Formulation of Mixture of oils was more effective showing complete seed protection i.e.no growth of fungi and insects upto 150 days storage than salphos (150 days). While salphos controlled only maximum three fungi (A. terreus, C. dematium, F. moniliforme). The formulated oils mixture did not have any adverse effect on the chickpea seeds and increased their shelf life.


Assuntos
Cicer/microbiologia , Armazenamento de Alimentos , Óleos Voláteis/farmacologia , Sementes/microbiologia , Anethum graveolens/química , Antifúngicos/farmacologia , Aspergillus/efeitos dos fármacos , Aspergillus flavus/efeitos dos fármacos , Óleo de Cravo/farmacologia , Fusarium/efeitos dos fármacos , Óleos Vegetais/farmacologia
3.
Biomed Res Int ; 2021: 5535570, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33997002

RESUMO

Anethum graveolens, belonging to the family Apiaceae, has been extensively used for medicinal and therapeutic purposes since long. Plants encompass rich number of effective constituents with less toxicity. Thus, nowadays, the attempts are being made to search plant constituents that can prevent and reverse the chronic diseases, such as cancer. In this study, an in vitro antioxidant and anticancer efficacies of Anethum graveolens (AG-ME) were studied on human breast (MCF-7), lung (A-549), and cervical (HeLa) carcinoma cell lines. The antioxidant efficacies of AG-ME were evaluated by total antioxidant, DPPH radical scavenging, H2O2 scavenging, and ferrous reducing antioxidant assays. Further, the anticancer potential of AG-ME was also determined against different cancer cell lines. The AG-ME exhibited strong antioxidant activities as observed by antioxidant assays. AG-ME also showed a dose-dependent anticancer/cytotoxic potential against MCF-7, A-549, and HeLa cell lines. The AG-ME-induced reduction in GSH and increase in SOD activities indicates the role of oxidative stress in AG-ME-induced MCF-7 cell death. The results also exhibited that AG-ME triggered ROS production and significantly reduced MMP level. Moreover, a dose-dependent increase in caspase-3 and caspase-9 activities suggests that the AG-ME-induced MCF-7 cell death is caspase-dependent. Together, the present study provides reasoning and reassurance for the uses of A. graveleons for medical purposes as an antioxidant and anticancer agent. Additional investigations are required to examine biological and anticancer activities under an in vivo system to discover a possible beneficial use of AG-ME against diseases.


Assuntos
Anethum graveolens/química , Antineoplásicos Fitogênicos/farmacologia , Antioxidantes/farmacologia , Neoplasias da Mama/metabolismo , Caspases/metabolismo , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Feminino , Humanos , Estresse Oxidativo/efeitos dos fármacos , Compostos Fitoquímicos/farmacologia
4.
J Food Biochem ; 45(6): e13741, 2021 06.
Artigo em Inglês | MEDLINE | ID: mdl-33904177

RESUMO

Hydroxyl radical (• OH) scavenging capacity of aqueous dill (Anethum graveolens L.) shoot (ADSh) extract was assessed using electron paramagnetic resonance (EPR) spectroscopy. ADSh extract (at concentrations of 0.5 and 10 mg/ml) exerted high (OH) radical scavenging power. ADSh extract was further fractionated on Diaion HP-20 column to yield five fractions. EPR spin-trapping assay revealed fraction 4 (eluted with 75% aq. MeOH) to possess (• OH) radical scavenging capacity over a concentration range (0.01-10 mg/ml), whereas fraction 2 (eluted with 25% aq. MeOH) appeared to be pro-oxidant at concentration 0.01 mg/ml. UPLC-PDA-ESI-MS metabolite profiling of ADSh extract revealed 87 metabolites, of which 64 compounds were identified in fraction 4, the most active fraction. Furthermore, ADSh extract demonstrated a hepatoprotective effect against acetaminophen (APAP)-induced hepatotoxicity in rats. Pretreatment of rats with ADSh extract (200 mg/kg b.wt) markedly attenuated the increased in the serum hepatic enzyme levels. It also increased free glutathione level and total antioxidant capacity in the serum of treated rats. [Correction added on May 3, 2021, after first online publication: "rates" has been changed to "rats" in the previous sentence.] Additionally, levels of (TNF-α and IL-1ß) were back to almost normal levels compared to the control group. The above findings suggest that ADSh extract has a protective effect against APAP-induced liver damage.


Assuntos
Anethum graveolens , Doença Hepática Induzida por Substâncias e Drogas , Acetaminofen/toxicidade , Animais , Antioxidantes/farmacologia , Doença Hepática Induzida por Substâncias e Drogas/tratamento farmacológico , Doença Hepática Induzida por Substâncias e Drogas/prevenção & controle , Extratos Vegetais/farmacologia , Extratos Vegetais/uso terapêutico , Ratos
5.
PLoS One ; 16(3): e0248662, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33765084

RESUMO

In controlled environments, crop models that incorporate environmental factors can be developed to optimize growth and development as well as conduct cost and/or resource use benefit analyses. The overall objective of this study was to model growth and development of dill 'Bouquet' (Anethum graveolens), parsley 'Giant of Italy' (Petroselinum crispum), and watercress (Nasturtium officinale) in response to photosynthetic daily light integral (DLI) and mean daily temperature (MDT). Plants were grown hydroponically in five greenhouse compartments with MDTs ranging from 9.7 to 27.2 °C under 0%, 30%, or 50% shade cloth to create DLIs ranging from 6.2 to 16.9 mol·m‒2·d‒1. MDT and DLI interacted to influence dill fresh mass and height, and watercress maximum quantum yield of dark adapted leaves (Fv/Fm), height, and branch number while only MDT affected dill leaf number and watercress fresh mass and branch length. Besides dry matter concentration (DMC), parsley was influenced by MDT and not DLI. Increasing MDT from ≈10 to 22.4 °C (parsley) or 27.2 °C (dill and watercress), linearly or near-linearly increased fresh mass. For dill, increasing DLI decreased fresh mass when MDT was low (9.7 to 13.9 °C) and increased fresh mass when MDT was high (18.4 to 27.2 °C). DMC of dill, parsley, and watercress increased as MDT decreased or DLI increased, indicating a higher proportion of plant fresh mass is water at higher MDTs or lower DLIs. With these data we have created growth and development models for culinary herbs to aid in predicting responses to DLI and MDT.


Assuntos
Anethum graveolens , Hidroponia/métodos , Petroselinum , Fotoperíodo , Temperatura , Anethum graveolens/crescimento & desenvolvimento , Anethum graveolens/metabolismo , Petroselinum/metabolismo , Fotossíntese , Folhas de Planta/crescimento & desenvolvimento
6.
Pest Manag Sci ; 77(7): 3349-3357, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33773022

RESUMO

BACKGROUND: Determining the environmental behavior and fate of chemical compounds during technological processing of plants is a task of great significance nowadays. However, the current knowledge is still incomplete for unique herbal matrices belonging to minor crops. The research in this article presents, for the first time, the dissipation kinetics and processing behavior of carboxamide boscalid (BOS) and stobilurin pyraclostrobin (PYR) fungicides during glasshouse dill (Anethum graveolens L.) cultivation. RESULTS: The half-lives (t1/2 ) of BOS and PYR after application at the recommended and double dosage were in the range: 1.62-2.01 days in plant and 2.08-4.85 days in soil, respectively. The processing behavior in dill was estimated after washing, hot air drying and drying in sunlight without/with pretreatment. Processing factors (PFs) were above 1 after drying (PF = 1.24-1.39 hot air; PF = 1.15-1.28 sunlight) and below this value when the washing step was applied (PF = 0.31-0.42 hot air; PF = 0.21-0.34 sunlight), indicating the highest effectiveness of reduction, up to 73% BOS and 79% PYR. CONCLUSION: BOS/PYR residues at pre-harvest intervals after both doses were below European Union (EU) maximum residue limits (MRLs). The highest effectiveness was noted for drying carried out with the washing step, which has a great influence on the concentration of residues in the final product. The findings can supplement PF databases not set for minor crops and can be used to establish MRLs and determine human exposures more accurately in risk assessment studies. © 2021 Society of Chemical Industry.


Assuntos
Anethum graveolens , Fungicidas Industriais , Resíduos de Praguicidas , Compostos de Bifenilo , Fungicidas Industriais/análise , Humanos , Cinética , Niacinamida/análogos & derivados , Resíduos de Praguicidas/análise , Solo , Estrobilurinas
7.
Food Chem ; 344: 128707, 2021 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-33267985

RESUMO

In this study, different variants of egg-free mayonnaise containing free and immobilized Lactobacillus plantarum LBRZ12 cells and essential oils taken from basil and dill were prepared. The composition and antimicrobial properties of essential oils were investigated. The main constituents of basil oil were methyl chavicol (36.81%), methyl eugenol (20.40%), ß-linanool (14.35%), eugenol (10.55%), and L(-)-carvone (39.05%), whereas dill oil contained mostly d-limonene (21.11%) and α-phellandrene (22.68%). The essential oils exhibited strong antimicrobial activity against all test-microorganisms. The mayonnaise variants were kept refrigerated for 40 days and changes in pH, concentration of viable cells of lactobacilli, microbiological, and organoleptic characteristics were monitored. The pH decreased from 6.5 to 4.5 over the period of storage. The number of undesired microflora in mayonnaise preserved with lactobacilli and essential oils decreased significantly (0 after the 20th day) indicating their effectiveness as biological preservatives. The mayonnaise variants demonstrated pleasant organoleptic characteristics, thus meet customers' requirements.


Assuntos
Anethum graveolens/química , Conservação de Alimentos/métodos , Lactobacillus plantarum/metabolismo , Óleos Voláteis/farmacologia , Óleos Vegetais/farmacologia , Preservação Biológica/métodos , Ocimum
8.
Int J Biol Macromol ; 167: 516-527, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-33279565

RESUMO

A novel water-soluble polysaccharide named AGP1 was successfully isolated from seeds of Anethum graveolens by hot water extraction and further purified by DEAE-Sepharose chromatography. AGP1 has a relative molecular weight of 2.1 104 Da determined by Ultra-high-performance liquid chromatography (UHPLC). The AGP1 characterization was investigated by chemical and instrumental analysis including gas chromatography mass spectrometry (GC-MS), Fourier transform infrared (FT-IR) spectroscopy and X-ray diffraction. Results showed that AGP1 was mainly composed of glucose, galactose, mannose and arabinose in a molar percent of 54.3, 23.8, 14.7 and 7.2, respectively. The thermogravimetry analysis (TGA) and the differential scanning calorimetry (DSC) were used and showed that AGP1 has good thermal stability until 275 °C. Moreover, the purified polysaccharide demonstrated an appreciable in vitro antioxidant potential. The addition of the AGP1, particularly at 0.3% (w/w), in turkey sausages instead of ascorbic acid, as preservative, reduced the lipid peroxidation, preserved the pH and color and improved the bacterial stability during cold storage at 4 °C for 12 days. Overall, the results showed that the AGP1 deserves to be developed as functional and bioactive components for the food and nutraceutical industries.


Assuntos
Anethum graveolens/química , Antioxidantes/química , Conservantes de Alimentos/química , Polissacarídeos/química , Antioxidantes/farmacologia , Configuração de Carboidratos , Cromatografia Líquida de Alta Pressão , Suplementos Nutricionais , Conservantes de Alimentos/farmacologia , Peroxidação de Lipídeos/efeitos dos fármacos , Polissacarídeos/farmacologia , Sementes/química , Solubilidade , Termogravimetria
9.
Food Chem ; 344: 128714, 2021 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-33272762

RESUMO

Using dill (Anethum graveolens L.) as a model herb, we reveal novel associations between metabolite profile and sensory quality, by integrating non-target metabolomics with sensory data. Low night temperatures and exposure to UV-enriched light was used to modulate plant metabolism, thereby improving sensory quality. Plant age is a crucial factor associated with accumulation of dill ether and α-phellandrene, volatile compounds associated with dill flavour. However, sensory analysis showed that neither of these compounds has any strong association with dill taste. Rather, amino acids alanine, phenylalanine, glutamic acid, valine, and leucine increased in samples exposed to eustress and were positively associated with dill and sour taste. Increases in amino acids and organic acids changed the taste from lemon/grass to a more bitter/pungent dill-related taste. Our procedure reveals a novel approach to establish links between effects of eustressors on sensory quality and may be applicable to a broad range of crops.


Assuntos
Anethum graveolens/metabolismo , Metabolômica/métodos , Paladar , Verduras/metabolismo
10.
Braz J Biol ; 81(1): 183-188, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-32074174

RESUMO

Gastrointestinal nematodes are responsible for great economic losses in sheep raising, and their control has long been carried out almost exclusively by the administration of anthelmintics, which have led to serious resistance problems. In the search for alternative control measures, phytotherapic research is highlighted. The aim of this study was to evaluate the action of Anethum graveolens (dill) essential oil on different stages of Haemonchus contortus life cycle, as well its cytotoxicity MDBK (Madin-Darby bovine kidney) cells. H. contortus larvae and eggs were obtained from infected sheep feces, and essential oil extracted from plant seeds through the Clevenger apparatus. 9.4, 4.7, 2.35, 1.17. 0.58 and 0.29 mg/mL concentrations were evaluated. The Egg Hatch Inhibition (HI), Larval Development Inhibition (LDI) and Larval Migration Inhibition (LMI) techniques were used. Thybendazole 0.025 mg/mL in HI and Levamisole 0.02 mg/mL in the LDI and LMI tests were used as positive controls, while distilled water and a Tween 80 solution were used as positive negative controls. The inhibition results obtained for the highest oil concentration were: HI 100%, LDI 98.58% and LMI 63.7%, differing (𝑝 <0.05) from negative controls. Main A. graveolens oil components present in 95.93% of the total oil were Dihydrocarvone (39.1%), Carvone (22.24%), D-Limonene (16.84%), Apiol (10.49%) and Trans-dihydrocarvone (7.26%). Minimum A. graveolens essential oil concentrations required to inhibit 50% (IC50) of egg hatching, larval development and larval migration were 0.006 mg/mL, 2.536 mg/mL and 3.963 mg/mL, respectively. Cell viability in MDBK (Madin-Darby bovine kidney) cells, when incubated with A. graveolens essential oil, was 86% for the highest (9.4 mg/mL) and 99% for the lowest concentration (0.29 mg/mL). A. graveolens essential oil, according to the results obtained in this study, is a promising alternative in sheep gastrointestinal nematode control.


Assuntos
Anethum graveolens , Anti-Helmínticos , Haemonchus , Óleos Voláteis , Animais , Anti-Helmínticos/farmacologia , Bovinos , Larva , Óleos Voláteis/farmacologia , Extratos Vegetais/farmacologia , Ovinos
11.
Food Sci Technol Int ; 27(1): 32-45, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32501117

RESUMO

Thin-layer drying behaviour of dill leaves samples under three different infrared radiation power intensities was investigated. Diffusion Approach was found to be the best model that describes the drying behaviour of dill leaves. Effective diffusivity values were 6.97 × 10-9, 6.84 × 10-9 and 8.96 × 10-9 m2/s for power intensities of 1790, 1970 and 2070 W/m2, respectively. According to the first and second law efficiencies, the infrared drying system was more efficient at higher power intensities (p < 0.05). However, the total colour change was maximum at the highest power intensity. For the investigated infrared drying conditions, 1970 W/m2 was recommended as the best-infrared power intensity applied for drying of dill leaves by taking into account both performance analyses and quality changes.


Assuntos
Anethum graveolens , Dessecação , Manipulação de Alimentos , Alimentos em Conserva , Folhas de Planta , Cor , Manipulação de Alimentos/métodos , Alimentos em Conserva/normas , Raios Infravermelhos , Folhas de Planta/química , Temperatura
12.
Environ Sci Pollut Res Int ; 28(5): 5704-5713, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32968907

RESUMO

The accumulation of heavy metal in the soil is a serious concern for sustainable food production due to their toxic effects on plants and other living things. The strategies are required on urgent bases for the management of metal-contaminated soils. Thus, the microbes from the genus Pseudomonas were characterized for different traits and lead (Pb)-resistant ability and their effects were assessed on growth, photosynthesis, antioxidant capacity, and Pb uptake by dill (Anethum graveolens L.). Furthermore, soil basal respiration and induced respiration in soil were also assessed under microbes and Pb stress. Among the tested three strains, Pseudomonas P159 and P150 were more tolerant to Pb stress than Pseudomonas P10, whereas P159 showed the highest values for phosphorus (P), siderophore, auxin, and hydrogen cyanide production. The bacterial inoculation increased the plant shoot dry weights, carbohydrates, proline, and chlorophyll contents under Pb stress. The catalase (CAT) and peroxidase (POD) activities of the plants were higher in bacterial-treated plants than control. The bacterial inoculation decreased Pb concentration in plants, and the response varied with the type of microbes. The bacterial strains enhanced the soil basal and induced respiration than respective Pb treatments alone. Overall, Pseudomonas P159 is potentially suitable for the remediation of Pb-contaminated soils. Graphical abstract.


Assuntos
Anethum graveolens , Poluentes do Solo , Antioxidantes , Bactérias , Biodegradação Ambiental , Chumbo , Solo , Poluentes do Solo/análise
13.
Complement Ther Clin Pract ; 41: 101251, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33130423

RESUMO

BACKGROUND: It is well documented that prolonged labor is associated with complications. This systematic review aimed to study the effect of Anethum Graveolens Linn (Dill) on the duration of labor. METHODS: We conducted a search on PubMed, Scopus, Cochrane central, Web of Science, and MEDLINE. All randomized controlled trials and observational studies evaluating the effect of Dill seeds on labor were recruited. The mean differences (MD) with 95% CI were calculated. RESULTS: Two RCTs showed that using Dill seeds could significantly reduce duration of the first stage (MD = -43.66, 95% CI [-52.99, - 34.33]), second stage (MD = -15.76, 95% CI [-20.06, -11.47]) and third stage of labor (MD = -1.79, 95% CI [-2.62, -0.95]). Two retrospective cohort studies showed a significant reduction in the first, and second stages of labor. CONCLUSION: Using Dill seeds could reduce the duration of labor. Using this herb in low-risk pregnant women is recommended. Systematic review registration number: CRD42020145225.


Assuntos
Anethum graveolens , Trabalho de Parto , Fitoterapia , Feminino , Humanos , Trabalho de Parto/efeitos dos fármacos , Gravidez , Gestantes , Estudos Retrospectivos , Sementes
14.
J Food Biochem ; 44(12): e13485, 2020 12.
Artigo em Inglês | MEDLINE | ID: mdl-33015851

RESUMO

High-cholesterol diet (HCD) is correlated with Alzheimer's disease (AD) and impairment of memory. This study investigated beneficial therapeutic effects of Dill tablet and Ocimum basilicum (Basil) aqueous extract on hypercholesterolemia-induced cognitive deficits and oxidative stress in hippocampus tissues of rats. Hippocampal Aß(1-42) level was measured. The gene expression levels of superoxide dismutase and inducible-nitric oxide synthase were determined in hippocampus. Cognitive functions were examined and oxidative status was evaluated in serum and hippocampus. Phytochemical properties and in vitro antioxidant activity of Basil extract were assessed. HCD significantly increased serum cholesterol, induced deposition of Aß plaque, altered hippocampus morphology, and impaired memory function, whereas receiving Basil extract or Dill tablet increased antioxidant potency in serum and hippocampus and normalized HCD-induced deleterious effects. Basil extract and Dill tablet may exhibit their beneficial effects in AD by lowering serum cholesterol and evoking antioxidant system in the brain. PRACTICAL APPLICATIONS: Dill tablet and Basil aqueous extract lowered serum cholesterol in hypercholesterolemic animal models, therefore, they can be used as hypocholesterolemic agents. These edible herbs significantly retarded deposition of Aß plaque and normalized hippocampal morphology, thus, they favorably protected hippocampus tissue from deleterious effects-induced by hypercholesterolemia. Dill tablet and Basil aqueous extract also corrected oxide-redox balance and normalized HCD-induced oxidative stress to some extent and significantly improved impairments in learning and memory suggesting that these medicinal plants can be considered as surrogate therapeutic agents for the synthetic medicines in the treatment of AD and in postponement of its complications.


Assuntos
Anethum graveolens , Ocimum basilicum , Animais , Cognição , Extratos Vegetais/farmacologia , Extratos Vegetais/uso terapêutico , Ratos , Comprimidos
15.
Int J Cosmet Sci ; 42(5): 444-451, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32583541

RESUMO

BACKGROUND: The loss of structural elastin due to intrinsic and extrinsic ageing results in the skin's inability to stretch and recoil (decrease in elasticity) and manifests as loss of skin firmness and sagging. While other extracellular matrix (ECM) components such as collagen and hyaluronic acid are continually synthesized and assembled through life, elastic fibres are not. Elastic fibre assembly and functionality require fibre cross-linking, induced by the lysyl oxidase-like (LOXL) enzymes, which sharply decrease during ageing. OBJECTIVE: To evaluate the enhanced elastogenic effect of a blackberry-dill extract combination, which was hypothesized to induce elastin fibre component synthesis, fibre cross-linking and reduce elastin fibre degradation. METHODS: The blackberry and the dill extracts were tested separately and in combination to confirm single ingredient bioactivity and synergistic benefits. Human skin explants, dermal fibroblasts, elastase assays, ELISAs, quantitative real-time PCRs and spectrofluorometer measurements were used. Moreover, a double-blinded, placebo-controlled clinical study was carried out to assess skin elasticity using Cutometer and histologically from biopsies. RESULTS: The blackberry extract induced elastin gene expression, elastin promoter activity and inhibited elastic fibre degradation by matrix metalloproteinases (MMPs) 9 and 12. The dill extract induced elastin, collagen and LOXL1 gene expression, resulting in enhanced fibre cross-linking in human skin explants. Clinically, the blackberry and dill combination treatment displayed synergistic pro-elasticity activity as compared to each ingredient alone and placebo. CONCLUSION: Taken together, these results demonstrated the two multimodal plant-based extracts complemented each other in terms of bioactivity and resulted in a synergistic elastogenesis induction.


Assuntos
Anethum graveolens/química , Elasticidade , Extratos Vegetais/farmacologia , Rubus/química , Pele/efeitos dos fármacos , Animais , Método Duplo-Cego , Sinergismo Farmacológico , Elastina/metabolismo , Matriz Extracelular/metabolismo , Humanos , Ratos , Pele/metabolismo , Espectrometria de Fluorescência
16.
Recent Pat Drug Deliv Formul ; 14(3): 223-232, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32479252

RESUMO

BACKGROUND: Many topical agents are available in the market, which interfere with the pigmentation process at different levels. They are often known to cause side effects ranging from irritation to tumor over chronic use. OBJECTIVE: The present study was designed to develop and characterize an anti blemish cream containing herbal oils. METHODS: A herbal cream was formulated using dill, nagarmotha and black cumin oil and subjected to evaluation of its anti blemish potential against stress augmented UV-B rays-induced hyperpigmentation. Topical oil in water type of creams containing 2%, 4% and 6% of each oil was formulated using herbal oils. The formulated cream was characterized for solubility, pH, particle size, grittiness, viscosity, stability, phase separation, shelf life and spreadability, and found to be stable. Acute dermal toxicity was carried out individually for dill, nagarmotha and black cumin oil according to the OECD guidelines 402. Hyperpigmentation was induced in all the experimental animals by stress-augmented UV-B irradiation method. The animals were treated for 30 days (twice daily) with standard and test formulations by topical administration, whereas the disease group was left untreated. The skin of the animals was subjected to photographical study as well as grading for pigmentation and irritation before and after treatment. After the treatment period, the serum antioxidant levels were estimated and histopathology, histochemical studies of skin were performed. RESULTS: The animals treated with test formulations containing 2%, 4%, and 6% of herbal oil showed significant improvement in pigmentation compared to disease control as it is evident in photographic biochemical, histopathological and histochemical studies. CONCLUSION: Thus, it was concluded that the developed anti-blemish cream containing herbal oils possesses significant anti-blemish potential. This study necessitates further evaluations in human subjects as it could have a high positive therapeutic value in the treatment of hyperpigmentation.


Assuntos
Hiperpigmentação/tratamento farmacológico , Óleos Vegetais/administração & dosagem , Preparações Clareadoras de Pele/administração & dosagem , Administração Cutânea , Anethum graveolens , Animais , Cyperus , Modelos Animais de Doenças , Composição de Medicamentos , Feminino , Hiperpigmentação/etiologia , Hiperpigmentação/patologia , Nigella sativa , Óleos Vegetais/química , Ratos Wistar , Creme para a Pele , Preparações Clareadoras de Pele/química , Pigmentação da Pele/efeitos da radiação , Estresse Psicológico/complicações , Raios Ultravioleta
17.
Trials ; 21(1): 483, 2020 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-32503652

RESUMO

BACKGROUND: The objective of this study was to investigate the effects of Anethum graveolens (dill) powder supplementation on glycemic control, lipid profile, some antioxidants and inflammatory markers, and gastrointestinal symptoms in patients with type 2 diabetes. MATERIALS AND METHODS: In this study, 42 patients with type 2 diabetes were randomly allocated to intervention and control groups and received either 3 g/day dill powder or placebo (3 capsules/day, 1 g each). Fasting blood sugar, insulin, homeostatic model assessment of insulin resistance, lipid profile, high-sensitivity C-reactive protein, total antioxidant capacity, malondialdehyde and gastrointestinal symptoms were measured in all of the subjects at baseline and postintervention. RESULTS: The dill powder supplementation significantly decreased the mean serum levels of insulin, homeostatic model assessment of insulin resistance, low-density lipoprotein cholesterol, total cholesterol and malondialdehyde in the intervention group in comparison with the baseline measurements (P < 0.05). Furthermore, the mean serum levels of high-density lipoprotein and total antioxidant capacity were significantly increased in the intervention group in comparison with the baseline measurement (P < 0.05). Colonic motility disorder was the only gastrointestinal symptom whose frequency was significantly reduced by supplementation (P = 0.01). The mean changes in insulin, low-density lipoprotein cholesterol, total cholesterol and malondialdehyde were significantly lower in the intervention group than in the control group (P < 0.05). In addition, the mean changes in high-density lipoprotein were significantly higher in the intervention group than in the control group (P < 0.05). CONCLUSION: Dill powder supplementation can be effective in controlling the glycemic, lipid, stress oxidative and gastrointestinal symptoms in patients with type 2 diabetes. TRIAL REGISTRATION: Iran Clinical Trials Registry: IRCT20120704010181N12. Registered on 12 May 2018.


Assuntos
Anethum graveolens , Antioxidantes/farmacologia , Biomarcadores/sangue , Diabetes Mellitus Tipo 2/sangue , Extratos de Tecidos/farmacologia , Adulto , Glicemia/metabolismo , Proteína C-Reativa/metabolismo , Suplementos Nutricionais , Feminino , Trato Gastrointestinal/efeitos dos fármacos , Humanos , Resistência à Insulina , Irã (Geográfico) , Lipídeos/sangue , Masculino , Malondialdeído/sangue , Pessoa de Meia-Idade , Estresse Oxidativo/efeitos dos fármacos , Pós/farmacologia
18.
Biol Trace Elem Res ; 198(2): 583-591, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32152873

RESUMO

The cadmium (Cd) is a toxic heavy metal that induces oxidative stress in both humans and animals. The plant phenolic compounds are capable of alleviating the toxicity of heavy metals. The encapsulation of plant bioactive compounds using nanoemulsion technology could enhance their bioefficacy. In this study, the protective effects of Anethum graveolens seed's oil nanoemulsion (AGN) against cadmium-induced oxidative stress in mice were studied. The results showed that the major bioactive compounds of essential oil were carvone and limonene. The result of particle size analysis revealed the pseudo-spherical droplets with nanometer size (148.8 ± 9.48 nm), homogenous dispersion, and physical colloidal stability. The Cd intoxication in mice (5 mg/kg BW for 30 days) reduced the body weight gain; however, treatment of the mice with different concentration of AGN (0.125, 0.25, and 0.5 mg/L, 30 days) through drinking water improved the body weight loss, liver Cd deposition, lipid peroxidation, cellular antioxidant redox potential, and inflammation in the liver, kidney, and brain of the mice challenged by cadmium-induced oxidative stress. The results of the present study revealed that drinking the essential oil of Anethum graveolens nanoemulsion containing carvone and limonene could be a promising strategy to protect the tissues against cadmium-induced oxidative damage.


Assuntos
Anethum graveolens , Cádmio , Animais , Antioxidantes/farmacologia , Cádmio/toxicidade , Peroxidação de Lipídeos , Camundongos , Estresse Oxidativo , Sementes
19.
Biomolecules ; 10(2)2020 01 23.
Artigo em Inglês | MEDLINE | ID: mdl-31979322

RESUMO

Elastic fibers (90% elastin, 10% fibrillin-rich microfibrils) are synthesized only in early life and adolescence mainly by the vascular smooth muscle cells through the cross-linking of its soluble precursor, tropoelastin. Elastic fibers endow the large elastic arteries with resilience and elasticity. Normal vascular aging is associated with arterial remodeling and stiffening, especially due to the end of production and degradation of elastic fibers, leading to altered cardiovascular function. Several pharmacological treatments stimulate the production of elastin and elastic fibers. In particular, dill extract (DE) has been demonstrated to stimulate elastin production in vitro in dermal equivalent models and in skin fibroblasts to increase lysyl oxidase-like-1 (LOXL-1) gene expression, an enzyme contributing to tropoelastin crosslinking and elastin formation. Here, we have investigated the effects of a chronic treatment (three months) of aged male mice with DE (5% or 10% v/v, in drinking water) on the structure and function of the ascending aorta. DE treatment, especially at 10%, of aged mice protected pre-existing elastic lamellae, reactivated tropoelastin and LOXL-1 expressions, induced elastic fiber neo-synthesis, and decreased the stiffness of the aging aortic wall, probably explaining the reversal of the age-related cardiac hypertrophy also observed following the treatment. DE could thus be considered as an anti-aging product for the cardiovascular system.


Assuntos
Envelhecimento , Aminoácido Oxirredutases/metabolismo , Anethum graveolens/química , Aorta/efeitos dos fármacos , Cardiomegalia/tratamento farmacológico , Extratos Vegetais/farmacologia , Animais , Aorta/metabolismo , Fenômenos Biomecânicos , Pressão Sanguínea , Peso Corporal , Cardiomegalia/metabolismo , Fibroblastos/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Tamanho do Órgão , Extratos Vegetais/química , RNA/metabolismo , Pele/metabolismo , Tropoelastina/metabolismo
20.
Nat Prod Res ; 34(13): 1923-1926, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30661393

RESUMO

A comprehensive study on essential oil samples extracted from Ridolfia segetum Moris (Apiaceae) collected in Tarquinia (Italy) is reported. In this study, a 30-hour, fractionated, steam distillation procedure for essential oil preparation was applied. The gas chromatographic-mass spectrometry analysis showed monoterpene o-cymene and phenylpropanoid dill-apiol as the major essential oil's constituents revealing a new chemotype dependent on extraction duration. Great impact of the duration of the distillation process on chemical profile of essential oil was observed; prolonged distillation gives chemically more diverse essential oil samples. Preliminary microbiological evaluations of the essential oils samples revealed some activity, although not high, against Candida albicans.


Assuntos
Apiaceae/química , Óleos Voláteis/análise , Anethum graveolens , Candida albicans/efeitos dos fármacos , Destilação/métodos , Cromatografia Gasosa-Espectrometria de Massas/métodos , Itália , Monoterpenos/análise , Óleos Voláteis/química , Óleos Voláteis/farmacologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...