Anticipatory and consummatory neural correlates of monetary and music rewarding stimuli.

Most of the literature on the neural bases of human reward and punishment processing has used monetary gains and losses, but less is known about the neurophysiological mechanisms underlying the anticipation and consumption of other types of rewarding stimuli. In the present study, EEG was recorded from 19 participants who completed a modified version of the Monetary Incentive Delay (MID) task. During the task, cues providing information about potential future outcomes were presented to the participants. Then, they had to respond rapidly to a target stimulus to win money or listening to pleasant music, or to avoid losing money or listening to unpleasant music. Results revealed similar responses for monetary and music cues, with increased activity for cues indicating potential gains compared to losses. However, differences emerged in the outcome phase between money and music. Monetary outcomes showed an interaction between the type of the cue and the outcome in the Feedback Related Negativity and Fb-P3 ERPs and increased theta activity increased for negative feedbacks. In contrast, music outcomes showed significant interactions in the Fb-P3 and theta activities. These findings suggest similar neurophysiological mechanisms in processing cues for potential positive or negative outcomes in these two types of stimuli.

Differential psychophysiological responses associated with decision-making in children from different socioeconomic backgrounds.

This study examined how socioeconomic status (SES) influences on decision-making processing. The roles of anticipatory/outcome-related cardiac activity and awareness of task contingencies were also assessed. One hundred twelve children (Mage = 5.83, SDage = 0.32; 52.7% female, 51.8% low-SES; data collected October-December 2018 and April-December 2019) performed the Children's Gambling Task, while heart rate activity was recorded. Awareness of gain/loss contingencies was assessed after completing the task. Distinct decision-making strategies emerged among low and middle/high-SES children. Despite similar awareness levels between SES groups, future-oriented decision-making was linked solely to the middle/high-SES group. Somatic markers did not manifest unequivocally. However, contrasting cardiac patterns were evident concerning feedback processing and the association between anticipatory activity and awareness (low: acceleration vs. middle/high: deceleration). Results are interpreted from an evolutionary-developmental perspective.

Disability-free life expectancy and life expectancy in good self-rated health in Chile: Gender differences and compression of morbidity between 2009 and 2016.

BACKGROUND: Chile has one of the highest life expectancies at 60 years in South America. This study was aimed to determine healthy life expectancies among Chilean older people, according to self-rated health and disability, and to explore gender differences. METHODS: Data from the National Survey of Health (2009 and 2016) were used to estimate prevalence of less than good self-rated health and disability among people aged 60 years and above. Health expectancies were calculated with the Sullivan method. RESULTS: In both years, women expected to live a lower proportion of their life expectancy in good self-rated health (54.5% [95% CI 50.0-58.8] for men and 37.6% [95% CI 34.3-40.8] for women in 2009; 46.1% [95% CI 42.6-49.7] for men and 38.5% [95% CI 35.6-41.4] for women in 2016). Life expectancy in less than good self-rated health increased for men (9.4 years [95% CI 8.4-10.3] in 2009; 11.5 years [95% CI 10.7-12.2]). Women expected to live a lower proportion of their remaining life without disabilities (65.3% [95% CI 61.2-69.4] for men and 44.9% [95% CI 41.9-47.9] for women in 2009; 71.9% [95% CI 68.7-75.0] for men and 61.1% [95% CI 58.5-63.8] for women in 2016). In 2016, disability-free life expectancy increased among women, but they still had a higher life expectancy with mild disability (2.8 years [95% CI 2.3-3.4] for men and 6.0 years [95% CI 5.4-6.7] for women). CONCLUSIONS: Women expected to spend more years in less than good self-rated health and disabled. There was an expansion of life expectancy in less than good SRH among men and a compression of disability in both sexes. The high proportion of years expected to be lived in less than good self-rated health and gender differences in disability-free life expectancy of older adults should be addressed by public health policies in Chile.

Intense craving for appetizing foods: validation and standardization of the Food Cravings Questionnaire-Trait in Mexico.

INTRODUCTION: Food craving is a motivational and physiological response for eating specific foods, mainly with high caloric content. To assess food craving, self-reports, inventories and questionnaires are used. The Food Cravings Questionnaire-Trait is multi-dimensionally structured and has been validated in several countries, since it is sensitive and adaptable to contextual-cultural changes. OBJECTIVES: To validate and standardize the Food Cravings Questionnaire-Trait in adults of Mexico City. METHOD: Non-experimental, cross-sectional, randomized study of 1059 subjects of both genders, between 18 and 84 years of age; 71.86 % of the female gender. Psychometric properties were examined with exploratory and confirmatory factor analyses. RESULTS: The domains of the questionnaire were reduced and the items were reorganized differently from the original version. The confirmatory factor analysis showed an adequate fit and acceptable standardization of factors. High internal consistency was found for the global questionnaire (a = 0.973 and rho = 0.975) for each one of the domains. CONCLUSION: This study determines the viability of the Food Cravings Questionnaire for the population of Mexico City.

INTRODUCCIÓN: El food craving o "ansia por comer" es una respuesta motivacional y fisiológica por comer alimentos específicos, principalmente con alto contenido calórico. Para evaluarlo se usa, entre otros, el Food Craving Questionnaire Trait, estructurado multidimensionalmente y validado en diversos países, el cual ha mostrado ser sensible y adaptable a los cambios contextuales-culturales. OBJETIVOS: Validar y estandarizar el Food Craving Questionnaire-Trait en adultos de la Ciudad de México. MÉTODO: Estudio no experimental, transversal y aleatorizado de 1059 sujetos de uno y otro sexo, entre 18 y 84 años; 71.86 % del sexo femenino. Se examinaron propiedades psicométricas con análisis factoriales exploratorios y confirmatorios. RESULTADOS: Se redujeron los factores del cuestionario y los ítems se reorganizaron de forma diferente al original. El análisis factorial confirmatorio mostró ajuste adecuado y estandarización aceptable de los factores. Se encontró alta consistencia interna para el cuestionario global (a = 0.973 y rho = 0.975) para cada uno de los factores. CONCLUSIÓN: Este estudio determina la viabilidad del Food Craving Questionnaire para población de la Ciudad de México.

Deseo intenso por alimentos apetecibles: validación y estandarización del Food Craving Questionnaire-Trait en México / Intense craving for appetizing foods: validation and standardization of the Food Cravings Questionnaire-Trait in Mexico

Resumen Introducción: El food craving o “ansia por comer” es una respuesta motivacional y fisiológica por comer alimentos específicos, principalmente con alto contenido calórico. Para evaluarlo se usa, entre otros, el Food Craving Questionnaire Trait, estructurado multidimensionalmente y validado en diversos países, el cual ha mostrado ser sensible y adaptable a los cambios contextuales-culturales. Objetivos: Validar y estandarizar el Food Craving Questionnaire-Trait en adultos de la Ciudad de México. Método: Estudio no experimental, transversal y aleatorizado de 1059 sujetos de uno y otro sexo, entre 18 y 84 años; 71.86 % del sexo femenino. Se examinaron propiedades psicométricas con análisis factoriales exploratorios y confirmatorios. Resultados: Se redujeron los factores del cuestionario y los ítems se reorganizaron de forma diferente al original. El análisis factorial confirmatorio mostró ajuste adecuado y estandarización aceptable de los factores. Se encontró alta consistencia interna para el cuestionario global (a = 0.973 y rho = 0.975) para cada uno de los factores. Conclusión: Este estudio determina la viabilidad del Food Craving Questionnaire para población de la Ciudad de México.

Abstract Introduction: Food craving is a motivational and physiological response for eating specific foods, mainly with high caloric content. To assess food craving, self-reports, inventories and questionnaires are used. The Food Cravings Questionnaire-Trait is multi-dimensionally structured and has been validated in several countries, since it is sensitive and adaptable to contextual-cultural changes. Objectives: To validate and standardize the Food Cravings Questionnaire-Trait in adults of Mexico City. Method: Non-experimental, cross-sectional, randomized study of 1059 subjects of both genders, between 18 and 84 years of age; 71.86 % of the female gender. Psychometric properties were examined with exploratory and confirmatory factor analyses. Results: The domains of the questionnaire were reduced and the items were reorganized differently from the original version. The confirmatory factor analysis showed an adequate fit and acceptable standardization of factors. High internal consistency was found for the global questionnaire (a = 0.973 and rho = 0.975) for each one of the domains. Conclusion: This study determines the viability of the Food Cravings Questionnaire for the population of Mexico City.

Random access to palatable food stimulates similar addiction-like responses as a fixed schedule, but only a fixed schedule elicits anticipatory activation.

Restricted intermittent food access to palatable food (PF) induces addiction-like behaviors and plastic changes in corticolimbic brain areas. Intermittent access protocols normally schedule PF to a fixed time, enabling animals to predict the arrival of PF. Because outside the laboratory the presence of PF may occur in a random unpredictable manner, the present study explored whether random access to PF would stimulate similar addiction-like responses as observed under a fixed scheduled. Rats were randomly assigned to a control group without chocolate access, to ad libitum access to chocolate, to fixed intermittent access (CH-F), or to random unpredictable access (CH-R) to chocolate. Only the CH-F group developed behavioral and core temperature anticipation to PF access. Both groups exposed to intermittent access to PF showed binge eating, increased effort behaviors to obtain chocolate, as well as high FosB/ΔFosB in corticolimbic areas. Moreover, FosB/ΔFosB in all areas correlated with the intensity of binge eating and effort behaviors. We conclude that both conditions of intermittent access to PF stimulate addiction-like behaviors and FosB/ΔFosB accumulation in brain reward areas; while only a fixed schedule, which provides a time clue, elicited anticipatory activation, which is strongly associated with craving behaviors and may favor relapse during withdrawal.

Verb-mediated anticipatory eye movements in people with Down syndrome.

BACKGROUND: Children and adults with neurotypical development employ linguistic information to predict and anticipate information. Individuals with Down syndrome (DS) have weaknesses in language production and the domain of grammar but relative strengths in language comprehension and the domain of semantics. What is not clear is the extent to which they can use linguistic information, as it unfolds in real time, to anticipate upcoming information correctly. AIMS: To investigate whether children and young people with DS employ verb information to predict and anticipate upcoming linguistic information. METHODS & PROCEDURES: A preferential looking task was performed, using an eye-tracker, with children and teenagers with DS and a typically developing (TD) control group matched by sex and mental age (average = 5.48 years). In each of 10 trials, two images were presented, a target and a distractor, while participants heard a phrase that contained a semantically informative verb (e.g., 'eat') or an uninformative verb (e.g., 'see'). OUTCOMES & RESULTS: Both DS and TD control participants could anticipate the target upon hearing an informative verb, and prediction skills were positively correlated with mental age in those with DS. CONCLUSIONS & IMPLICATIONS: This work demonstrates for the first time that children and teenagers with DS can predict linguistic information based on semantic cues from verbs, and that sentence processing is driven by predictive relationships between verbs and arguments, as in children with typical development. Clinicians can take advantage of these prediction skills, using them in therapy to support weaker areas.

Top-Down Control of Alpha Phase Adjustment in Anticipation of Temporally Predictable Visual Stimuli.

Alpha oscillations (8-14 Hz) are proposed to represent an active mechanism of functional inhibition of neuronal processing. Specifically, alpha oscillations are associated with pulses of inhibition repeating every ∼100 msec. Whether alpha phase, similar to alpha power, is under top-down control remains unclear. Moreover, the sources of such putative top-down phase control are unknown. We designed a cross-modal (visual/auditory) attention study in which we used magnetoencephalography to record the brain activity from 34 healthy participants. In each trial, a somatosensory cue indicated whether to attend to either the visual or auditory domain. The timing of the stimulus onset was predictable across trials. We found that, when visual information was attended, anticipatory alpha power was reduced in visual areas, whereas the phase adjusted just before the stimulus onset. Performance in each modality was predicted by the phase of the alpha oscillations previous to stimulus onset. Alpha oscillations in the left pFC appeared to lead the adjustment of alpha phase in visual areas. Finally, alpha phase modulated stimulus-induced gamma activity. Our results confirm that alpha phase can be top-down adjusted in anticipation of predictable stimuli and improve performance. Phase adjustment of the alpha rhythm might serve as a neurophysiological resource for optimizing visual processing when temporal predictions are possible and there is considerable competition between target and distracting stimuli.

Microsaccadic behavior when developing a complex dynamical activity.

Microsaccade are sensitive to changes of perceptual inputs as well as modulations of cognitive states. There are just a few works analyzing microsaccade while subjects are processing complex information and fewer when doing predictions about upcoming events. To evaluate whether contextual predictability would change microsaccadic behavior, we evaluated microsaccade of twenty one persons when reading 40 regular sentences and 40 proverbs. Analysis of microsaccade during reading proverbs and regular sentences revealed that microsaccade rate on words before maxjump, during maxjump and words after maxjump varied depending on the kind of sentence and on the word predictability. Maxjump was defined as the word with the largest difference between the cloze predictability of two consecutive words. Low and high predictable words demanded less or more microsaccade on words previous, during and on maxjump depending of the semantic context and of the readers' predictions of upcoming words.In summary, the present study shows that microsaccade' rate evidenced significant differences when reading proverbs and regular sentences. Hence, evaluation of microsaccade during reading sentences with different contextual predictability might provide information about specific effect of cue attention on complex task.

Medial prefrontal cortex stimulation modulates irony processing as indexed by the N400.

In this study, we investigated whether the ERP responses observed during a verbal irony comprehension task might represent the cortical manifestation of the Medial Prefrontal Cortex (MPFC) activity. We performed a tDCS-EEG study in which we analyzed the effects of tDCS polarities (anode, cathode, sham) over the MPFC during a verbal irony task. We presented visual short stories portraying everyday situations followed by written statements in either an ironic or literal condition, whose meaning was referred to in the previous context. We manipulated the valence of the stimuli by presenting positive sentences or negative sentences in the ironic and literal conditions. The results revealed that the participants who received the anodal stimulation showed no differences in the N400 amplitude in response to the literal and the ironic condition. This could suggest that anodal stimulation has modulatory effects on N400 responses during irony comprehension. Our results indicated that the MPFC might be critical in accessing ironic information at the initial stage of irony comprehension. Finally, we found that the ironic compliments were more difficult to understand compared to the literal ones, suggesting that irony comprehension is affected by the valence of the information presented.

"Era óbvio que isso iria acontecer": considerações sobre o viés retrospectivo / "It was obvious that this would happen": consideration about the hindsight bias

O viés retrospectivo é o fenômeno de perceber e avaliar eventos diferentemente, uma vez que eles tenham ocorrido. Dada uma falha cognitiva, pessoas tendem a ter distorções da memória e a produzir falsas sensações de inevitabilidade e previsibilidade. Os objetivos deste trabalho foram fazer uma revisão de literatura do viés retrospectivo e discutir as implicações teóricas e práticas do tema. Uma pesquisa, realizada nas principais bases de dados do país, não encontrou nenhum artigo relacionado com o tema. A partir de uma seleção das principais pesquisas internacionais sobre o assunto, foi feita uma análise que mostrou os níveis cognitivos do viés retrospectivo, suas implicações e as estratégias usadas para atenuar o viés. Nas considerações finais, são discutidos o impacto e a importância do conhecimento desse fenômeno no dia a dia de tomadores de decisão e cidadãos, assim como a necessidade de se explorar o tema em pesquisas nacionais.

Hindsight bias is the phenomenon of perceiving and evaluating events differently once they are occurred. Given a cognitive flaw, people tend to have distortions of memory and produce false sensations of inevitability and predictability. The objective of this work is to make a literature review of the hindsight bias and to discuss both theoretical and practical implications of the theme. A search conducted in the Brazilian's main databases did not find any articles related to the topic. From a selection of the main international studies on the subject, an analysis was made showing the cognitive levels of the hindsight bias, its implications and the strategies used to attenuate the bias. In the final considerations, the impact and importance of day-to-day knowledge of the phenomenon and the need to explore the subject in national research are discussed.

Temporal Anticipation Based on Memory.

The fundamental role that our long-term memories play in guiding perception is increasingly recognized, but the functional and neural mechanisms are just beginning to be explored. Although experimental approaches are being developed to investigate the influence of long-term memories on perception, these remain mostly static and neglect their temporal and dynamic nature. Here, we show that our long-term memories can guide attention proactively and dynamically based on learned temporal associations. Across two experiments, we found that detection and discrimination of targets appearing within previously learned contexts are enhanced when the timing of target appearance matches the learned temporal contingency. Neural markers of temporal preparation revealed that the learned temporal associations trigger specific temporal predictions. Our findings emphasize the ecological role that memories play in predicting and preparing perception of anticipated events, calling for revision of the usual conceptualization of contextual associative memory as a reflective and retroactive function.

La información situacional probabilística en el deporte: un metaanálisis / Situational probability information in sport: A meta-analysis

Resumen El objetivo del estudio es cuantificar el efecto de la información situacional probabilística (ISP) sobre el rendimiento deportivo y en concreto sobre el inicio en el tiempo de respuesta y la precisión de los deportistas. Existe evidencia científica de que los deportistas de mayor nivel deportivo desarrollan la habilidad perceptiva para usar la ISP, realizando comportamientos anticipatorios, si bien se desconoce la magnitud de dicho efecto. Se realiza un metaanálisis de efectos aleatorios para cuantificar el tamaño del efecto (TE) de la ISP en el comportamiento motor de los deportistas. Los resultados muestran un TE medio final grande de 1.29 para el tiempo de respuesta (en 7 estudios y 252 participantes) y de 1.31 para la precisión (en nueve investigaciones y 292 participantes), seleccionados de acuerdo con los criterios establecidos. Con la limitación del reducido número de estudios analizados (n = 9), los resultados indican que los deportistas expertos anticipan más respuestas y con más precisión que los noveles debido al uso de la ISP. Se recomienda entrenar perceptivamente la identificación y el uso de la ISP a nivel deportivo, ya que aquellos deportistas que hagan un mejor uso de esta información contextual podrán anticipar sus respuestas con precisión.

Abstract The aim of the study is to quantify the effect of Situational Probability Information (SPI) on athletic performance, specifically on the onset of the response time and the accuracy of athletes. There is scientific evidence that shows that higher sport level athletes develop the perceptive skills to use SPI, thereby performing anticipatory behaviors. However, the magnitude of this effect remains unknown. A meta-analysis of random effects was carried out to quantify the effect size (ES) of SPI on the motor behavior of athletes. The results showed an overall final mean ES of 1.29 for the response time (in 7 studies and 252 participants), and 1.31 for accuracy (in 9 studies and 292 participants), according to the criteria established. With the limitation of the low number of studies analyzed (n = 9), the results suggest that expert athletes anticipate more frequently and with higher accuracy than beginners, due to the use of SPI. It is recommended to train in the identification and use of the SPI in the sports domain, as athletes who use this contextual information more effectively could anticipate the responses with precision.

Nível de antecipação em jogadores de tênis novatos e inexperientes pelo método de oclusão temporal / Nivel de antecipación en jugadores de tenis novatos e inexpertos por el método de oclusión temporal / Anticipation level of novices and inexperienced tennis players by temporal occlusion paradigm

O objetivo do estudo foi avaliar o nível de antecipação em jogadores novatos e inexperientes através do método de oclusão temporal, com a utilização de um jogador experto com performance profissional no saque de tênis. O resultado principal demonstrou diferenças significativas entre os grupos (p<0,05), os novatos antecipam melhor nos estágios mais anteriores do movimento, à -0,3s e -0,4s do momento de contato da raquete com a bola no saque. Conclui-se que há aprendizagem perceptiva nesta fase e que o tema da antecipação deve ser incluído dentro dos planos de ensino e treinamento de iniciantes no esporte.

The study objective was to evaluate anticipation level in novices and inexperienced players trough temporal occlusion paradigm, using an expert player with professional performance on tennis serve. The main result shows significant differences between groups (p<0.05), novices anticipates better on the earlier stages of the movement, at -0.3s and -0.4s from racquet and ball contact. The conclusion points out the existence of perceptual learning in these first stages of learning and that anticipation content should be part of education and training plans for sports beginners.

El objetivo del estudio fue evaluar el nivel de anticipación en jugadores novatos e inexpertos por medio del método de oclusión temporal, con la utilización de un jugador experto con rendimiento profesional en el servicio del tenis. El resultado principal demostró diferencias significativas entre los dos grupos (p<0,05), los novatos anticipan mejor en los momentos más anteriores del movimiento, a -0,3s e -0,4s del momento de contacto de la raqueta con la pelota en el servicio. Se concluye que existe aprendizaje perceptivo en esta fase y que el tema de la anticipación debe ser incluido en los planes de enseñanza y entrenamiento de iniciantes en el deporte.

Time-place learning in the zebrafish (Danio rerio).

Animals exhibit activity cycles that repeat over days. The most noteworthy cyclical behaviors are related to forraging, which generally occur at the same times and locations. The synchronization of animal activities via the association of different places at different times for the occurrence of relevant biological events is known as time-place learning (TPL). In the present study, we used zebrafish (Danio rerio) to test time-place learning based on a different stimulus: social reinforcement. Fish were not only able to associate time and place of the social stimulus, but also displayed anticipatory activity prior to the arrival of the stimulus. Furthermore, we show that the group of conspecifics is an relevant stimulus for time-place learning tasks, while other studies have only used food.

Influence of Therapeutic Approach in the TENS-induced Hypoalgesia.

INTRODUCTION: The present study aimed to determine how the therapist's approach about intervention may influence transcutaneous electrical nerve stimulation (TENS)-induced hypoalgesia. METHODS: One hundred and sixty-one pain-free individuals agreed to participate in this study and had their demographics, perceived pain intensity, pressure pain threshold, anxiety level, and the state of anxiety inventory score measured. Subsequently, participants were randomly assigned into 6 study groups, 3 active and 3 placebo TENS associated with positive, negative, or neutral approaches about electrical stimulation, as given by the investigator. After the treatment, all parameters were reassessed. RESULTS: Active TENS-treated participants receiving either positive or neutral expectations about intervention showed a significant increase in pressure pain threshold (P<0.02) compared with pretreatment; however, this was not observed in the active TENS group when associated with negative expectations. The intensity of perceived pain was significantly reduced (P<0.02) only in the active TENS groups in association with either positive or neutral expectations. There was no significant difference in any of the variables assessed in the groups receiving placebo TENS intervention. DISCUSSION: The negative expectations induced prior to the proposed intervention promoted unfavorable outcomes with respect to the analgesic properties of TENS, suggesting that the approach taken by the physical therapist should be used to convey positive expectations and avoid those negatives, to promote more efficacious treatment.

Escalation in high fat intake in a binge eating model differentially engages dopamine neurons of the ventral tegmental area and requires ghrelin signaling.

Binge eating is a behavior observed in a variety of human eating disorders. Ad libitum fed rodents daily and time-limited exposed to a high-fat diet (HFD) display robust binge eating events that gradually escalate over the initial accesses. Intake escalation is proposed to be part of the transition from a controlled to a compulsive or loss of control behavior. Here, we used a combination of behavioral and neuroanatomical studies in mice daily and time-limited exposed to HFD to determine the neuronal brain targets that are activated--as indicated by the marker of cellular activation c-Fos--under these circumstances. Also, we used pharmacologically or genetically manipulated mice to study the role of orexin or ghrelin signaling, respectively, in the modulation of this behavior. We found that four daily and time-limited accesses to HFD induce: (i) a robust hyperphagia with an escalating profile, (ii) an activation of different sub-populations of the ventral tegmental area dopamine neurons and accumbens neurons that is, in general, more pronounced than the activation observed after a single HFD consumption event, and (iii) an activation of the hypothalamic orexin neurons, although orexin signaling blockage fails to affect escalation of HFD intake. In addition, we found that ghrelin receptor-deficient mice fail to both escalate the HFD consumption over the successive days of exposure and fully induce activation of the mesolimbic pathway in response to HFD consumption. Current data suggest that the escalation in high fat intake during repeated accesses differentially engages dopamine neurons of the ventral tegmental area and requires ghrelin signaling.

Nurses' expectations and perceptions of a redesigned Electronic Health Record.

When a new Electronic Health Record is implemented or modifications are made, the full acceptance by end users depends on their expectations and perceptions about the possible benefits and the potential impacts on care quality. The redesign of an electronic nurse chart should consider the inherent characteristics of nurses' practice and the variables that may influence the implementation and use of the new chart. In this study, a qualitative method evaluated nurses' expectations and perceptions about the implementation impacts of a redesigned nurse chart in an electronic health record at Hospital Italiano de Buenos Aires. Seventy-four nurses participated in three operative groups. Following ground theory, three analytic dimensions were found: impact at work, communication and chart quality. In addition, time was a recurrent topic. Nurses found it difficult to think positively if reduction in time of documentation was not assured.

Progressive anticipation in behavior and brain activation of rats exposed to scheduled daily palatable food.

Scheduled and restricted access to a palatable snack, i.e. chocolate, elicits a brief and strong anticipatory activation and entrains brain areas related with reward and motivation. This behavioral and neuronal activation persists for more than 7days when this protocol is interrupted, suggesting the participation of a time-keeping system. The process that initiates this anticipation may provide a further understanding of the time-keeping system underlying palatable food entrainment. The aim of this study was to analyze how this entraining protocol starts and to dissect neuronal structures that initiate a chocolate-entrained activation. We assessed the development of anticipation of 5g of chocolate during the first 8days of the entrainment protocol. General activity of control and chocolate-entrained rats was continuously monitored with movement sensors. Moreover, motivation to obtain the chocolate was assessed by measuring approaches and interaction responses toward a wire-mesh box containing chocolate. Neuronal activation was determined with c-Fos in reward-related brain areas. We report a progressive increase in the interaction with a box to obtain chocolate parallel to a progressive neuronal activation. A significant anticipatory activation was observed in the prefrontal cortex on day 3 of entrainment and in the nucleus accumbens on day 5, while the arcuate nucleus and pyriform cortex reached significant activation on day 8. The gradual response observed with this protocol indicates that anticipation of a rewarding food requires repetitive and predictable experiences in order to acquire a temporal estimation. We also confirm that anticipation of palatable food involves diverse brain regions.