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1.
Environ Pollut ; 292(Pt B): 118450, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34740740

RESUMO

Aquaculture ecosystem is a hot-spot for antibiotic resistance genes (ARGs). Rice-crayfish co-culture was considered an eco-friendly aquaculture model and has been widely adopted in China. However, it is unclear whether rice-crayfish co-culture is one of the most eco-friendly models from the perspective of antibiotic resistance profiles. In this study, we evaluated the eco-friendliness of rice-crayfish co-culture, and compared this model with other aquaculture models, from the perspectives of antibiotics and ARG patterns, based on multi-omics and antibiotic profiles. Results showed that the nutrient levels, antibiotic concentrations, dominant microbial genera and ARG patterns in the rice-crayfish co-culture model were profoundly different from the other three aquaculture models (crab only aquaculture model, crayfish only aquaculture model, and crab-crayfish co-culture models). Specifically, the rice-crayfish co-culture model has significantly lower diversity of ARGs and lower potential risks of ARGs when compared to the other aquaculture models. Nutrient and antibiotic concentrations were the important environmental factors for shaping ARG patterns, but compared with environmental factors, the effects of mobile genes and bacteria community on the proliferation and transmission of ARGs were stronger. This study has deepened our understanding of ARGs in freshwater aquaculture ecosystem, and suggested that rice-crayfish co-culture model is a relatively eco-friendly aquaculture model when compared with the other aquaculture models.


Assuntos
Antibacterianos , Oryza , Animais , Aquicultura , Astacoidea , Técnicas de Cocultura , Ecossistema , Genes Bacterianos
2.
Chemosphere ; 286(Pt 3): 131926, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34435577

RESUMO

The redclaw crayfish, Cherax quadricarinatus, was introduced to Martinique Island for aquaculture purposes at the beginning of the 21st century, in an attempt to revitalize the freshwater crustacean aquaculture sector. Mainly due to its high economical value, it was intentionally released in the wild and was caught and sold by fishermen. Martinican rivers are polluted by chlordecone, considered as one of the worst Persistant Organic Pollutants (POP). Despite its dangerousness, it was used until 1993 in the French West Indies against a banana pest and was always found in the ecosystems. This study aimed to investigate the level of contamination in the muscle of crayfish caught in the wild, as well as the potential of bioconcentration and depuration in the C. quadricarinatus muscle. This study could allow us to quantify the risk for consumers but also, to evaluate a depuration process to reduce the risk related to its consumption. Using both in-vitro and in-situ experiments, results highlighted the importance of the chlordecone concentration in the water and the time of exposure to the pollutant. The bioconcentration seems to be very quick and continuous in crayfish muscle, as chlordecone can be detectable as early as 6 h of exposure, whatever the concentration tested. Finally, it appears that, even after 20 days of depuration in chlordecone-free water, chlordecone concentrations remained higher to the residual maximum limit (i.e. 20 ng/g wet weight), concluding that the decontamination of the muscle seems not very efficient, and the risk for the Martinican people could be serious.


Assuntos
Clordecona , Inseticidas , Animais , Astacoidea , Bioacumulação , Clordecona/análise , Ecossistema , Humanos , Inseticidas/análise , Martinica
3.
Parasitol Int ; 86: 102475, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-34610466

RESUMO

Ankylocythere sinuosa (Rioja, 1942), a symbiotic ostracod native to North America, was found from the Japanese mitten crab Eriocheir japonica (De Haan, 1835), a species native to Japan, collected from a pond in Shizuoka City, Shizuoka Prefecture, central Japan. Introduced North American crayfish Procambarus clarkii (Girard, 1852), which is a host of A. sinuosa in their native range, inhabits ponds sympatrically with Japanese mitten crabs, and it is thought that the ostracods transferred from the exotic crayfish to the native crabs. In recent years, along with the artificial transportation of crayfish around the world, their symbiotic ostracods also have been found on the body surfaces of exotic crayfish in Europe and Japan. However, no studies have confirmed the infestation of exotic ostracods on native crustaceans in the field. A wide range of developmental stages of A. sinuosa from juveniles to adults were found in Japanese mitten crabs, and mating individuals were also found. This strongly suggests that they can reproduce on the body surface of Japanese mitten crabs. In the future, it will be necessary to strengthen measures against alien species to prevent these exotic symbionts from infestating native ecosystems, and we also need to investigate the exact impact of this symbiont on Japanese mitten crabs.


Assuntos
Astacoidea/parasitologia , Crustáceos/fisiologia , Interações Hospedeiro-Parasita , Espécies Introduzidas , Simbiose , Animais , Feminino , Japão , Masculino
4.
Biol Trace Elem Res ; 200(1): 375-384, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33641053

RESUMO

The release of heavy metals to the environment increased dramatically with industrialization and rapid economic development, and they have accumulated in aquatic organisms. The current study aimed toe valuate the physiological, immunological, and histological changes of crayfish (Procambarus clarkii) as bio-indicator for water quality. Crayfishes of the filed study group were collected from a polluted area (Rosetta branch, Egypt), where the highest concentration for heavy metals in water was for zinc (Zn). Besides the field study group, other crayfishes were exposed to different doses of ZnSO4 (0, 203, and 406 mg L-g) corresponding to Zn concentration (0, 46.03, and 92.06 mg L-1) respectively in aquariums for consecutive 4 days. Heavy metal concentrations in field water sample were arranged as follows: Zn > Fe> Pb > Cu and Mn > Ni > Co > Cd. The result revealed that Zn bioaccumulation increases significantly with the increase of water Zn concentration among the tested groups compared to the control group, where the highest bioaccumulation in all studied tissues (hepatopancreas, gills, and muscles) was observed in the field group and Zn high-dose group. Also, there was a significant increase in the levels of hemolymph uric acid, urea, creatinine, glucose, aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase. Their highest concentrations were observed in the Zn high-dose group and the field group, while the levels of total protein, albumin, and cholesterol showed a significant decrease among the tested groups as compared with the control group. Their determined lowest concentrations were in the Zn high-dose group and field group. Among tested groups, total hemocytes and granulated hemocytes decreased significantly while hyaline hemocytes increased as compared with the control group. Histological damages were observed in hepatopancreas, gills, and muscles in the field and Zn groups. The present study showed that exposure to Zn caused physiological and histological changes in Procambarus clarkia. We assumed that Procambarus clarkia could be used as a sensitive bioindicator for monitoring water quality criteria.


Assuntos
Astacoidea , Metais Pesados , Animais , Monitoramento Ambiental , Brânquias/química , Metais Pesados/análise , Metais Pesados/toxicidade , Zinco
5.
Food Chem ; 372: 130935, 2022 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-34818725

RESUMO

The impacts of cold stress (4 â„ƒ for 0 h, 12 h, 24 h, 36 h and 48 h, respectively) on the components, structural and physical properties of myofibrillar protein (MP) gel from Procambarus clarkii were investigated. The physicochemical analysis indicated the secondary and tertiary structure of MP were unfolding to different degrees after cold stress when compared to the control. The MP gel hardness reached a maximum when the cold stress reached 24 h. Furthermore, the quantitative proteomics results indicated that 20 up-regulated differentially abundant proteins (DAPs) were detected in 24 h when compared to control, specifically include myosin light chain 1 (MLC1) and skeletal muscle actin 6. Additionally, the combined analysis confirmed that MLC1 and skeletal muscle actin 6 might play key roles in hardening shrimp meat under cold stress. The results could provide a theoretical reference for the changes in crayfish muscle quality during cold chain transportation.


Assuntos
Astacoidea , Proteômica , Animais , Astacoidea/genética , Resposta ao Choque Frio , Músculos
6.
Zootaxa ; 5017(1): 1-84, 2021 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-34810425

RESUMO

Presented is an account of the crayfish genus Creaserinus Hobbs, 1973 for Texas, based on materials gathered during a 13-year survey of the state. Home to Texas are six members of the genus, including C. hedgpethi (Hobbs, 1948) stat. rev., n. comb., which is resurrected from the synonymy of C. fodiens; and five species new to science described herein, including C. brevistylus n. sp., C. clausus n. sp., C. crenastylus n. sp., C. limulus n. sp., and C. trinensis n. sp. Collections of these species except for C. trinensis n. sp. were previously known and studied but ascribed to C. fodiens (Cottle, 1863), which is removed from the fauna of the state. Support for the taxonomic acts comes from genetics, morphology, distribution, life history, habitat, and syntopy. Accounts are provided for each species and include illustrations and information on distribution, color pattern, relationships, life history, ecology, size, variations, and crayfish associates. A key to the species in the state based on form I males is provided. Creaserinus limulus n. sp. is extraordinary in that a majority of its populations sampled have been composed mostly or entirely of females. Additions to the faunas of Texass neighboring states include C. clausus n. sp. (Louisiana), C. crenastylus n. sp. (Louisiana), and C. limulus n. sp. (Arkansas and Oklahoma).


Assuntos
Astacoidea , Ecossistema , Distribuição Animal , Animais , Feminino , Masculino , Texas
7.
Zootaxa ; 5026(3): 344-374, 2021 Aug 26.
Artigo em Inglês | MEDLINE | ID: mdl-34810926

RESUMO

A new species of freshwater crayfish in the southern hemisphere family Parastacidae is described from the Murray Darling Basin (MDB), eastern Australia. The Swamp Yabby, Cherax latimanus sp. nov., is found in the mid-Murray River area of the states of New South Wales and Victoria and is only the third species of Cherax found naturally occurring in the MDB. It is morphologically distinguished from all other species of Cherax by the presence of marginal mesial dactylar basal spines and extensive ventral propodal setation. It is found in sympatry with the widespread but morphologically similar Common Yabby, Cherax destructor Clark, 1936, but can be distinguished by a suite of morphometric and meristic characters that include a broad rostrum lacking an apical spine, very broad chelae with deep punctations on the lateral propodal edge and ventral propodal setation, two prominent dactylar teeth, one mesial carpal spine, dorsal meral spines present, no setae on carpal mesial margin, cervical spines absent or tiny, and by genetic data. Cherax latimanus is further diagnosed by its distinctive biology: it is not found in permanent aquatic habitats such as streams or billabongs, spending the majority of its time in extensive, terrestrial, burrow networks containing some water, in ephemerally wet habitats such as drainage lines, roadside drains, depressions, swamps and cleared areas of pasture in lowland to foothill areas. It is occasionally found in stream habitats but only during large flood events when burrow systems are inundated.


Assuntos
Astacoidea , Decápodes , Animais , Água Doce , Rios
8.
Dis Aquat Organ ; 146: 67-73, 2021 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-34617512

RESUMO

Batrachochytrium dendrobatidis (Bd) is a pathogenic fungus known to infect amphibians and crayfish. In crayfish, Bd causes gill tissue damage, and in some cases, mortality. Most research has focused on the amphibian-Bd system, so to date, little is known about the effects of Bd on the crayfish host. Here, we studied the effects of sublethal exposure to Bd and the metabolites produced by Bd on crayfish Procambarus alleni survival, gill damage, and oxygen consumption (as a proxy for mass-specific metabolic rate). Oxygen consumption increased 24 h post-exposure to live Bd, indicative of a stress response, followed by a decrease in oxygen consumption over time (χ21 = 6.39, p = 0.012). There was no difference in response when comparing the crayfish exposure to live Bd and Bd-metabolites alone (χ21 = 2.70, p = 0.101), indicating that the metabolites may have been the causative agent responsible for the response. Additionally, oxygen consumption decreased with gill damage (tissue recession) in Bd-exposed individuals. We found that high doses of Bd cause outright mortality in crayfish, and we show here that sublethal Bd-induced inhibition of oxygen consumption could negatively impact crayfish in the field, possibly reducing their overall fitness. More research is needed to understand this understudied host-parasite system. It is essential that we incorporate the disease dynamics associated with Bd and crayfish in conservation disease models, as this is the only way to develop comprehensive community-based models.


Assuntos
Astacoidea , Brânquias , Animais , Batrachochytrium , Respiração
9.
Ecotoxicol Environ Saf ; 227: 112911, 2021 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-34673411

RESUMO

Chromium (Cr) as a chromate anion has a strong redox capacity that seriously threatens the ecological environment and human health. Cr can contaminate water and impart toxicity to aquatic species. Procambarus clarkii is an important food source that once represented a large proportion of the aquaculture industry due to its rapid reproduction and high economic value. However, there have been reports on the death of P. clarkii due to heavy metal pollution. The underlying mechanism regarding heavy metal toxicity was studied in this paper. The transcriptome data of hemocytes extracted from P. clarkii injected with Cr were analyzed by high-throughput sequencing and compared to the control group. In total, 48,128,748 clean reads were obtained in the treatment group and 56,480,556 clean reads were obtained in the control group. The reads were assembled using Trinity and the identified unigenes were then annotated. Then, 421 differentially-expressed genes (DEGs) were found, 170 of which were upregulated and 251 downregulated. Many of these genes were found to be related to glutathione metabolism and transportation. The glutathione metabolic pathway of P. clarkii was thus activated by Cr exposure to detoxify and maintain body function. Validation of DEGs with quantitative real-time PCR confirms the changes in gene expression. Thus, this study provides data supporting a glutathione-focused response of P. clarkii to exposure to heavy metals.


Assuntos
Astacoidea , Clarkia , Animais , Antioxidantes , Astacoidea/genética , Cromo/toxicidade , Mecanismos de Defesa , Perfilação da Expressão Gênica , Humanos , Transcriptoma
10.
Appl Microbiol Biotechnol ; 105(19): 7451-7461, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34542688

RESUMO

Procambarus clarkii is the dominant economic variety of crayfish in China, and paddy field shrimp cultivation is an organic mode of traditional rice-fish cultivation, with paddy field shrimp being the country's prevailing aquatic product. However, little has been reported on the differences in meat quality and digestive ability between paddy field and pond fish. In this study, the muscle composition and digestive function regulation of P. clarkii in ponds and paddies were studied to explore the influence of paddy field culture on P. clarkii quality. The results showed that the muscle composition of paddy field shrimp was significantly changed, with increased protein and decreased lipid levels. Through the study of the hepatopancreas and intestinal microbial diversity of P. clarkii, we hypothesized that rice farming may cause changes in its bacterial spectrum, stimulate the digestive functions of its intestines and hepatopancreas, cause differential expression of multi-substance metabolic pathways, and ultimately result in the substances' deposition in its muscles. This study revealed the impact of rice cultivation on P. clarkii from the perspective of meta-metabolism, and it demonstrated the advantages of paddy field shrimp cultivation.Key points• We explored the influence of paddy field culture on P. clarkii quality.• Muscle composition of paddy field shrimp was significantly changed, with increased protein and decreased lipid levels in paddy field.• Rice farming caused changes in its bacterial spectrum and stimulated the digestive functions of hepatopancreas.


Assuntos
Astacoidea , Animais , China
11.
Med Pr ; 72(5): 605-610, 2021 Nov 19.
Artigo em Polonês | MEDLINE | ID: mdl-34491243

RESUMO

Haff disease is a group of symptoms caused by rhabdomyolysis following ingestion of some species of fish and crayfish. Pathophysiology remains unknown. Outbreaks of the Haff disease have been reported in many regions of the world. In this article we present the case of a 38-years-old patient, professional fisherman, suspected of suffering from Haff disease. He developed symptoms of diffuse myalgia, headache, chest pressure, brown-colored urine and elevated blood pressure. Predominant laboratory abnormalities were elevated serum creatine kinase and creatinine concentrations. During hospitalization the patient required the renal replacement therapy. As a result of applied procedures, clinical and laboratory improvement was achieved. The patient was discharged home in good general condition. The Haff disease should be included in the differential diagnosis of rhabdomyolysis. Detailed medical interview, especially questions about recently consumed food and occupational exposure, is crucial in making a proper diagnosis. Med Pr. 2021;72(5):605-10.


Assuntos
Epidemias , Rabdomiólise , Adulto , Animais , Astacoidea , Humanos , Masculino , Mialgia/epidemiologia , Rabdomiólise/diagnóstico , Rabdomiólise/epidemiologia , Alimentos Marinhos
12.
Biomolecules ; 11(8)2021 08 09.
Artigo em Inglês | MEDLINE | ID: mdl-34439846

RESUMO

The oomycete pathogen Aphanomyces astaci, also known as "crayfish plague", is an obligate fungal-like parasite of freshwater crustaceans and is considered responsible for the ongoing decline of native European crayfish populations. A. astaci is thought to secrete a wide array of effectors and enzymes that facilitate infection, however their molecular mechanisms have been poorly characterized. Here, we report the identification of AA15 lytic polysaccharide monooxygenases (LPMOs) as a new group of secreted virulence factors in A. astaci. We show that this enzyme family has greatly expanded in A. astaci compared to all other oomycetes, and that it may facilitate infection through oxidative degradation of crystalline chitin, the most abundant polysaccharide found in the crustacean exoskeleton. These findings reveal new roles for LPMOs in animal-pathogen interactions, and could help inform future strategies for the protection of farmed and endangered species.


Assuntos
Doenças dos Animais/microbiologia , Aphanomyces , Astacoidea/microbiologia , Infecções , Oxigenases de Função Mista/metabolismo , Fatores de Virulência/metabolismo , Animais , Aphanomyces/enzimologia , Aphanomyces/patogenicidade , Quitina/metabolismo , Infecções/microbiologia , Infecções/veterinária
13.
J Exp Biol ; 224(7)2021 04 01.
Artigo em Inglês | MEDLINE | ID: mdl-34424965

RESUMO

Orientation within turbulent odor plumes occurs across a vast range of spatial and temporal scales. From salmon homing across featureless oceans to microbes forming reproductive spores, the extraction of spatial and temporal information from chemical cues is a common sensory phenomenon. Yet, given the difficulty of quantifying chemical cues at the spatial and temporal scales used by organisms, discovering what aspects of chemical cues control orientation behavior has remained elusive. In this study, we placed electrochemical sensors on the carapace of orienting crayfish and measured, with fast temporal rates and small spatial scales, the concentration fluctuations arriving at the olfactory appendages during orientation. Our results show that the spatial aspects of orientation (turning and heading angles) are controlled by the temporal aspects of odor cues.


Assuntos
Sinais (Psicologia) , Odorantes , Animais , Astacoidea , Olfato
14.
Behav Processes ; 192: 104489, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34437979

RESUMO

In species whose social structure includes dominance relationships, individuals are likely to engage in frequent agonistic interactions with conspecifics, and these interactions can have substantial effects on participants. For example, 'winner' and 'loser' effects, whereby winning or losing a contest increases the probability of winning or losing subsequent encounters, have been described in many species. However, a smaller body of research has shown that winning or losing a contest can lead to additional behavioral changes that affect other domains of an individual's social experiences. Here, we report on an experiment designed to evaluate the effects of prior contests on subsequent mating interactions in the crayfish (Faxonius virilis). We presented males with mating opportunities either immediately following or 7 days after a contest with a conspecific male. We predicted that winners would be more likely to mate than losers, because of either or both winner/loser effects and differences in male competitiveness. We found that, when presented with a mating opportunity immediately following a contest, winning males were more likely to mate than were losing males. We also found that these differences had eroded within 7 days, such that there was no significant difference in the proportions of winners and losers that mated after that period. We concluded that the changes in mating behavior that we observed immediately after a contest were likely due to relatively short-term winner and loser effects, rather than any differences in the males' competitiveness, which would presumably be of longer duration.


Assuntos
Astacoidea , Comportamento Animal , Agressão , Animais , Humanos , Masculino
15.
Artigo em Inglês | MEDLINE | ID: mdl-34444507

RESUMO

Among brominated flame retardants (BFRs), polybrominateddiphenyl ethers (PBDEs) and hexabromocyclododecanes (HBCDs) were the most widely used in past decades. BFRs not being chemically bonded to polymers means they can easily leach from the products into the environment and bioaccumulate. Humans are exposed to flame retardants mainly through food consumption, especially fish and fish products. In the present study, the occurrence of PBDEs and HBCDs in freshwater fishes and crayfish from Lake Trasimeno (Umbria region, central Italy) was assessed according to monitoring plans recommended by European competent authorities. The dietary exposure of the central Italian population to such molecules was calculated, and the risk characterization and the benefit-risk evaluation were also assessed. A total of 90 samples were analyzed by means of gas and liquid chromatography associated with triple quadrupole mass spectroscopy. A total of 51% of samples were found positive for at least one of the congeners; the most frequently found molecule was BDE-47. The data on dietary exposure ranged from 0.138 to 1.113 pg/kg body weight/day for ∑PBDE and from 0.805 to 0.868 pg/kg body weight/day for ∑HBCD. The data show no health risks for the central Italian population consuming freshwater fish products from Lake Trasimeno in relation to exposure to PBDE and HBCD.


Assuntos
Retardadores de Chama , Animais , Astacoidea , Monitoramento Ambiental , Peixes , Retardadores de Chama/análise , Humanos , Itália , Lagos , Medição de Risco
16.
Int J Mol Sci ; 22(12)2021 Jun 16.
Artigo em Inglês | MEDLINE | ID: mdl-34208769

RESUMO

Early changes in hemocyte proteins in freshwater crayfish Pacifastacus leniusculus, in response to an injection with the fungal pattern recognition protein ß-1,3-glucan (laminarin) were investigated, as well as changes after saline (vehicle) injection and in naïve animals. Injection of saline resulted in rapid recruitment of granular hemocytes from surrounding tissues, whereas laminarin injection on the other hand induced an initial dramatic drop of hemocytes. At six hours after injection, the hemocyte populations therefore were of different composition. The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1. After injection with the ß-1,3-glucan, only three proteins were enhanced in expression, in comparison with saline-injected animals and uninjected controls. All of them may be associated with immune responses, such as a new and previously undescribed Kazal proteinase inhibitor. One interesting observation was that the clotting protein was increased dramatically in most of the animals injected with laminarin. The number of significantly affected proteins was very few after a laminarin injection when compared to uninjected and saline-injected crayfish. This finding may demonstrate some problematic issues with gene and protein expression studies from other crustaceans receiving injections with pathogens or pattern recognition proteins. If no uninjected controls are included and no information about hemocyte count (total or differential) is given, expressions data for proteins or mRNAs are very difficult to properly interpret.


Assuntos
Astacoidea/efeitos dos fármacos , Astacoidea/metabolismo , Hemócitos/metabolismo , beta-Glucanas/administração & dosagem , Animais , Proteínas de Artrópodes/genética , Proteínas de Artrópodes/metabolismo , Astacoidea/genética , Biomarcadores , Expressão Gênica , Hemócitos/citologia , Proteoma , Proteômica/métodos , RNA Mensageiro/genética
17.
J Environ Manage ; 297: 113314, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34298344

RESUMO

In this work the presence of different pharmaceuticals at Doñana National Park (Spain) and their main entry sources (input source or entry points) have been stated over the 2011-2016 years period. Twenty-three selected pharmaceuticals (corresponding to eight therapeutic families) were evaluated in crayfish and water samples from Doñana National Park (Spain) (six sampling points selected in order to cover different possible pollution sources into and surrounding the Park). The multiresidue determination was carried out using enzymatic-microwave assisted extraction prior to high performance liquid chromatography mass spectrometry detection. Sulphonamides (sulfadiazine, sulfamerazine, sulfamethazine, and sulfamethoxazole); trimethoprim, an antibiotic that is frequently co-administered with sulfamethoxazole; amphenicols (chloramphenicol, florfenicol and thiamphenicol); fluoroquinolones (ciprofloxacin, enrofloxacin, flumequine, danofloxacin, gatifloxacin, norfloxacin, marbofloxacin and grepafloxacin); penicillins (amoxicillin); tetracyclines (chlortetracycline and oxytetracycline); non-steroidal anti-inflammatory drugs (salicylic acid and ibuprofen); beta-blocker drugs (atenolol); and antiepileptics (carbamazepine) were analysed. Ciprofloxacin, ibuprofen, salicylic acid, flumequine, and carbamazepine were detected and/or quantified at some of the selected sampling points. A clear ecotoxicological risk to the ecosystem was demonstrated from the occurrence of ciprofloxacin in samples obtained after the punctual and massive presence of people inside the Park. Furthermore, flumequine and carbamazepine have been detected in Procambarus clarkii specimens in concentrations around 30 ng g-1 and 14 ng g-1, respectively, and their occurrence in the specimens could indicate the persistence of the discharge sources. The main source of pharmaceuticals into the Park might be the livestock farming activities, and the influence of urban wastewaters from surrounding villages does not seem to be very important.


Assuntos
Preparações Farmacêuticas , Poluentes Químicos da Água , Animais , Astacoidea , Biota , Ecossistema , Monitoramento Ambiental , Humanos , Parques Recreativos , Espanha , Poluentes Químicos da Água/análise
18.
Mar Genomics ; 58: 100842, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34217485

RESUMO

The genus Procambarus represents a diverse genus of freshwater crayfish that includes epigean species, stygobitic species, and at least one parthenogenic species. Despite its evolutionary, ecological, and economic importance, most genomic and transcriptomic resources for this genus are limited to a couple of model species. We sequenced the transcriptome of a non-model species, P. erythrops, a geographically restricted stygobitic species from Florida. RNA isolated from gill, muscle and eye tissue was pooled to create a de novo transcriptome assembly using Single Molecule Real-Time sequencing (PacBio), resulting in 19,442 full-length isoforms. The assembly has been deposited in the NCBI (BioProject PRJNA657230). These data will make an important contribution to the comparative study of transcriptome evolution in crayfish and crustaceans.


Assuntos
Astacoidea/genética , Transcriptoma , Animais , Florida , Análise de Sequência de RNA
19.
J Fish Dis ; 44(10): 1503-1513, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34227114

RESUMO

White spot syndrome virus (WSSV) is a serious pathogen threatening global crustacean aquaculture with no commercially available drugs. Herbal medicines widely used in antiviral research offer a rich reserve for drug discovery. Here, we investigated the inhibitory activity of 13 herbal medicines against WSSV in crayfish Procambarus clarkii and discovered that naringenin (NAR) has potent anti-WSSV activity. In the preliminary screening, the extracts of Typha angustifolia displayed the highest inhibitory activity on WSSV replication (84.62%, 100 mg/kg). Further, NAR, the main active compound of T. angustifolia, showed a much higher inhibition rate (92.85%, 50 mg/kg). NAR repressed WSSV proliferation followed a dose-dependent manner and significantly improved the survival of WSSV-challenged crayfish. Moreover, pre- or post-treatment of NAR displayed a comparable inhibition on the viral loads. NAR decreased the transcriptional levels of vital genes in viral life cycle, particularly for the immediately early-stage gene ie1. Further results showed that NAR could decrease the STAT gene expression to block ie1 transcription. Besides, NAR modulated immune-related gene Hsp70, antioxidant (cMnSOD, mMnSOD, CAT, GST), anti-inflammatory (COX-1, COX-2) and pro-apoptosis-related factors (Bax and BI-1) to inhibit WSSV replication. Overall, these results suggest that NAR may have the potential to be developed as preventive or therapeutic agent against WSSV.


Assuntos
Antivirais/farmacologia , Astacoidea/virologia , Flavanonas/farmacologia , Typhaceae/química , Vírus da Síndrome da Mancha Branca 1/efeitos dos fármacos , Animais , Antivirais/química , Flavanonas/química , Replicação Viral/efeitos dos fármacos , Vírus da Síndrome da Mancha Branca 1/fisiologia
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