Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 34.751
Filtrar
1.
Chemosphere ; 286(Pt 1): 131623, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34346348

RESUMO

The increasing demand for the development of sustainable strategies to utilize and process agro-industrial residues paves new paths for exploring innovative approaches in this area. Biotechnology based microbial transformations provide efficient, low cost and sustainable approaches for the production of value added products. The use of organic rich residues opens new avenues for the production of enzymes, pigments, biofuels, bioactive compounds, biopolymers etc. with vast industrial and therapeutic applications. Innovative technologies like strain improvement, enzyme immobilization, genome editing, morphological engineering, ultrasound/supercritical fluid/pulse electric field extraction, etc. can be employed. These will be helpful in achieving significant improvement in qualitative and quantitative parameters of the finished products. The global trend for the valorisation of biowaste has boosted the commercialization of these products which has transformed the markets by providing new investment opportunities. The upstream processing of raw materials using microbes poses a limitation in terms of product development and recovery which can be overcome by modifying the bioreactor design, physiological parameters or employing alternate technologies which will be discussed in this review. The other problems related to the processes include product stability, industrial applicability and cost competitiveness which needs to be addressed. This review comprehensively discusses the recent progress, avenues and challenges in the approaches aimed at valorisation of agro-industrial wastes along with possible opportunities in the bioeconomy.


Assuntos
Biocombustíveis , Resíduos Industriais , Reatores Biológicos , Biotecnologia , Indústrias
2.
Adv Exp Med Biol ; 1354: 299-314, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-34807448

RESUMO

Dairy and beef cattle make a vital contribution to global nutrition, and since their domestication, they have been continuously exposed to natural and artificial selection to improve production characteristics. The technologies of transgenesis and gene editing used in cattle are responsible for generating news characteristics in bovine breeding, such as alteration of nutritional components of milk and meat enhancing human health benefits, disease resistance decreasing production costs and offering safe products for human food, as well as the recombinant protein production of biomedical significance. Different methodologies have been used to generate transgenic cattle as bioreactors. These methods include the microinjection of vectors in pronuclear, oocyte or zygote, sperm-mediate transgenesis, and somatic cell nuclear transfer. Gene editing has been applied to eliminate unwanted genes related to human and animal health, such as allergy, infection, or disease, and to insert transgenes into specific sites in the host genome. Methodologies for the generation of genetically modified cattle are laborious and not very efficient. However, in the last 30 years, transgenic animals were produced using many biotechnological tools. The result of these modifications includes (1) the change of nutritional components, including proteins, amino acids and lipids for human nutrition; (2) the removal allergic proteins milk; (3) the production of cows resistant to disease; or (4) the production of essential proteins used in biomedicine (biomedical proteins) in milk and blood plasma. The genetic modification of cattle is a powerful tool for biotechnology. It allows for the generation of new or modified products and functionality that are not currently available in this species.


Assuntos
Leite , Técnicas de Transferência Nuclear , Animais , Animais Geneticamente Modificados , Reatores Biológicos , Biotecnologia , Bovinos , Feminino
3.
Bioresour Technol ; 343: 126097, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34626758

RESUMO

Lignocellulosic wastes were recently considered as biomass resources, however, its conversion to valuable products is still immature although researchers have put lots of effort into this issue. This article reviews the key challenges of the biorefinery utilizing lignocellulosic materials and recent developments to conquer those obstacles. Available biological techniques and processes, from the pretreatments of cellulosic materials to the valorization processes, were emphasized. Biological pretreatments, including hydrolysis using microbial consortia, fungi, enzymes, engineered bacterial/fungal strains, and co-culture systems, could enhance the release of reducing sugar. Resources recovery, including biogases, ethanol, butanol, PHA, etc., from lignocellulosic materials were also discussed, while the influences of composition of lignocellulosic materials and pretreatment options, applications of co-culture system, and integrated treatments with other wastes, were described. In the review, co-culture system and metabolic engineering are emphasized as the promising biological technologies, while perspectives are provided for their future developments.


Assuntos
Biocombustíveis , Lignina , Biomassa , Biotecnologia , Hidrólise
4.
Artigo em Inglês | MEDLINE | ID: mdl-34770089

RESUMO

The development of the biotech industry is in full swing, and consumers have begun to value biotech brands. Since biotech products often focus on the future or special benefits, consumers inevitably bear certain risks when purchasing biotech products, and their trust in the biotech brand will have an important impact on their purchase intention. Previous studies have lacked a targeted understanding of consumer trust in biotech brands and a discussion of cultural viewpoints. This study introduced the concept of personal connections in Chinese relationalism and trust strategies in Chinese society to address this gap. In-depth interviews and focus group discussions were conducted in collaboration with Company X, a listed Taiwanese cord blood company, to extract the key factors influencing consumer trust and purchase intention of biotech brands. After constructing the structure model, the study was validated using a structural equation model through investigation and survey. The findings indicated that consumer trust in biotech brands was constructed by a combination of kinship trust transfer and emergent trust transfer within the consumer relationship network, as well as institutional trust and professional trust outside the relationship network and that a significant positive correlation existed between consumer trust in biotech brands and purchase intention. The acquaintances within the consumer relationship network include not only relatives and friends but also health care workers and netizens that consumers come into contact with. In addition, kinship trust transfer and emergent trust transfer within the consumer relationship network have a greater impact on trust in biotech brands than the institutional trust and professional trust outside the relationship network. The findings of this study deepen the understanding of consumer trust in biotech brands across cultures, and suggest that future marketing communication should be expanded to include key players within the consumer relationship network.


Assuntos
Intenção , Confiança , Biotecnologia , Comportamento do Consumidor , Sangue Fetal , Humanos
5.
J Biotechnol ; 342: 128-138, 2021 Dec 10.
Artigo em Inglês | MEDLINE | ID: mdl-34743006

RESUMO

The present study demonstrates biotechnological applications of the lichen Pleurosticta acetabulum, specifically the production of large amounts of hydrogen even after the lichen exposure to extreme conditions such as a) extreme UVB radiation (1.7 mW/cm2 = 1000 J m-2 min-1) over different time periods (4, 20 & 70 h) and b) combined exposure of the lichen to high intensity UVB radiation and extreme low (-196 °C) or extreme high temperatures (+70 °C). The results highlight that the extremophilic and polyextremophilic behavior of lichens both in dehydrated and in regenerated form, under extreme conditions not necessarily recorded on earth, is compatible with their biotechnological uses. The lichen viability was measured using fluorescence induction techniques (OJIP-test), which record changes in the molecular structure and function of the photosynthetic mechanism, while its ability to produce molecular hydrogen was measured through thermal conductivity gas chromatography (GC-TCD) analysis. Hydrogen is a promising fuel for the future. The exciting result of a lichen micro-ecosystem is its ability to expel its moisture and remain in an inactive state, protecting itself from extreme conditions and maintaining its ability to high yield hydrogen production in a closed system, with the sole addition of water and without the need for additional energy. Our results expand the potential use of lichens for future biotechnological applications in extreme Earth environments, but also in environments on other planets, such as Mars, thus paving the way for astrobiotechnological applications.


Assuntos
Líquens , Biotecnologia , Ecossistema , Hidrogênio , Parmeliaceae , Temperatura
6.
Sheng Wu Gong Cheng Xue Bao ; 37(9): 3383-3396, 2021 Sep 25.
Artigo em Chinês | MEDLINE | ID: mdl-34622645

RESUMO

Accurately understanding the features and connotations of complex engineering problems is an important prerequisite for setting graduation requirements, constructing curriculum and designing teaching contents. By discussing the characteristics of complex engineering problems in the biological industry, this paper explored the demands for undergraduates in Yangtze river delta region, summarized the typical jobs and their requirements, and expounded the connotation of complex engineering problems contained in various typical tasks. On this basis, a gradual curriculum system was constructed, which included multiple stages of conceiving, formation and application, to cultivate the ability to solve complex engineering problems in the major of bioengineering. The curriculum coordinated the implementation of deep integration of industry and education, research feed back course construction, course team and advanced courses building up, professional associations covered all crews and students, supporting the ability training of solving complex engineering problems.


Assuntos
Currículo , Engenharia , Biotecnologia , Humanos , Indústrias , Resolução de Problemas
7.
Biosens Bioelectron ; 194: 113666, 2021 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-34600338

RESUMO

Intelligent microfluidics is an emerging cross-discipline research area formed by combining microfluidics with machine learning. It uses the advantages of microfluidics, such as high throughput and controllability, and the powerful data processing capabilities of machine learning, resulting in improved systems in biotechnology and chemistry. Compared to traditional microfluidics using manual analysis methods, intelligent microfluidics needs less human intervention, and results in a more user-friendly experience with faster processing. There is a paucity of literature reviewing this burgeoning and highly promising cross-discipline. Therefore, we herein comprehensively and systematically summarize several aspects of microfluidic applications enabled by machine learning. We list the types of microfluidics used in intelligent microfluidic applications over the last five years, as well as the machine learning algorithms and the hardware used for training. We also present the most recent advances in key technologies, developments, challenges, and the emerging opportunities created by intelligent microfluidics.


Assuntos
Técnicas Biossensoriais , Microfluídica , Biotecnologia , Humanos , Inteligência , Aprendizado de Máquina
8.
Front Public Health ; 9: 541191, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34660499

RESUMO

For-profit biotechnological and pharmaceutical companies have played an essential role in the research and development (R&D) of innovative medical products and drugs for many decades and embody a trillion-dollar industry. The past decades have been marked by an increase in growth of social non-profit biotechnology companies and organizations led by entrepreneurs committed to solve (global) health issues. In this review, we define the concept of social bioentrepreneurship and consider the potential impact of such ventures on global health. We analyse the current status of non-profit biotechnology and clarify the strategy, motivation, funding, and marketing techniques of these enterprises. We find that these non-profit ventures mainly focus on neglected and rare diseases by using different but also similar funding, marketing, and business strategy approaches to for-profit biotechnology enterprises. We also identify good leadership, multidisciplinary teams, and public awareness as key components to achieve long-term survival and higher success rates. Challenges faced by bioentrepreneurs include the lack of a clearly defined regulatory environment or governmental incentives to support their endeavors. Overall, with this qualitative data review and market analysis we draw a promising picture of social non-profit bioentrepreneurship and underscore its current and future impact on global health issues.


Assuntos
Saúde Global , Organizações sem Fins Lucrativos , Biotecnologia , Comércio
10.
Sheng Wu Gong Cheng Xue Bao ; 37(10): 3405-3410, 2021 Oct 25.
Artigo em Chinês | MEDLINE | ID: mdl-34708599

RESUMO

The international cooperation project "electricity-driven low energy and chemical input technology for accelerated bioremediation" (abridged as "ELECTRA") is jointly supported by National Nature Science Foundation of China (NSFC) and European Commission (EC). The ELECTRA consortium consists of 5 research institutions and universities from China and 17 European research institutions and universities, as well as high-tech companies of EC countries. ELECTRA focuses on researches of biodegradation of emerging organic compounds (EOCs) and novel environmental biotechnologies of low-energy and low-chemical inputs. The project has been successfully operated for 2 years, and has made important progresses in obtaining EOCs-degrading microbes, developing weak-electricity-accelerated bioremediation, and 3D-printing techniques for microbial consortium. The ELECTRA has promoted collaborations among the Chinese and European scientists. In the future, ELECTRA will overcome the negative impact of the COVID-19 pandemic and fulfill the scientific objectives through strengthening the international collaboration.


Assuntos
COVID-19 , Pandemias , Biodegradação Ambiental , Biotecnologia , Eletricidade , Humanos , SARS-CoV-2
11.
Sheng Wu Gong Cheng Xue Bao ; 37(10): 3414-3424, 2021 Oct 25.
Artigo em Chinês | MEDLINE | ID: mdl-34708601

RESUMO

With the transformation and revolution of the global plastics recycling system, recycling and upcycling of mixed plastics waste not only reduces the carbon emissions of plastics during its life cycle, but also addresses its potential ecological and environmental hazards. This article summarizes an international cooperation project, "MIXed plastics biodegradation and UPcycling using microbial communities" (MIX-UP) which was funded by the National Natural Science Foundation of China and the European Union (NSFC-EU) in 2019. The consortium of MIX-UP consists of 14 partners from European Union and China. Focusing on the global issue of "plastics pollution", this Sino-European MIX-UP project took the mixed waste of petroleum-based plastics (PP, PE, PUR, PET and PS) and bio-based plastics (PLA and PHA) as starting materials for biotechnological conversion into value-added, sustainable biomaterials. MIX-UP has three subprojects: 1) identification of plastics biodegradation pathway and design & engineering of key degrading elements, 2) construction and functional regulation of microbial consortia/enzyme cocktails with high-efficiency for degradation of plastics mixtures, 3) strategy of design and utilization of plastics degradation products for production of high value materials. Through NSFC-EU complementary and cross-disciplinary cooperation, MIX-UP proposes the engineering of a new-to-nature biological route for upcycling, a low carbon and sustainable bio-treatment that is different from the traditional physico-chemical treatment, which will empower the recycling industry to a new dimension. The implementation of the project will not only help to promote innovation and development in the field of biotechnology in China, but also contribute to the achievement of China's carbon neutral goal.


Assuntos
Microbiota , Plásticos , Biodegradação Ambiental , Biotecnologia , Carbono , União Europeia
12.
Molecules ; 26(20)2021 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-34684789

RESUMO

The use of additives has generated significant attention due to their extensive application in the microbially induced calcium carbonate precipitation (MICP) process. This study aims to discuss the effects of Na-montmorillonite (Na-MMT) on CaCO3 crystallization and sandy soil consolidation through the MICP process. Compared with the traditional MICP method, a larger amount of CaCO3 precipitate was obtained. Moreover, the reaction of Ca2+ ions was accelerated, and bacteria were absorbed by a small amount of Na-MMT. Meanwhile, an increase in the total cementing solution (TCS) was not conducive to the previous reaction. This problem was solved by conducting the reaction with Na-MMT. The polymorphs and morphologies of the CaCO3 precipitates were tested by using X-ray diffraction and scanning electron microscopy. Further, when Na-MMT was used, the morphology of CaCO3 changed from an individual precipitate to agglomerations of the precipitate. Compared to the experiments without Na-MMT in the MICP process, the addition of Na-MMT significantly reduced the hydraulic conductivity (HC) of sandy soil consolidated.


Assuntos
Bentonita/metabolismo , Carbonato de Cálcio/metabolismo , Sporosarcina/metabolismo , Bentonita/química , Biotecnologia , Carbonato de Cálcio/isolamento & purificação , Precipitação Química , Cristalização , Microscopia Eletrônica de Varredura , Areia/química , Solo/química , Sporosarcina/crescimento & desenvolvimento , Difração de Raios X
13.
Molecules ; 26(20)2021 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-34684788

RESUMO

It is known that Senna obtusifolia has been used in medicine since ancient times due to the content of many valuable compounds with a pro-health effect. One of them is betulinic acid, which is a pentacyclic triterpene with antimalarial, antiviral, anti-inflammatory and anticancer properties. In this work, a continuation of our previous research, an attempt was made to increase the level of betulinic acid accumulation by the cultivation of transgenic hairy roots that overexpress the squalene synthase gene in a 10 L sprinkle bioreactor with methyl jasmonate elicitation. We present that the applied strategy allowed us to increase the content of betulinic acid in hairy root cultures to the level of 48 mg/g dry weight. The obtained plant extracts showed a stronger cytotoxic effect on the U87MG glioblastoma cell line than the roots grown without elicitors. Additionally, the induction of apoptosis, reduction of mitochondrial membrane potential, chromosomal DNA fragmentation and activation of caspase cascades are demonstrated. Moreover, the tested extract showed inhibition of topoisomerase I activity.


Assuntos
Acetatos/farmacologia , Antineoplásicos Fitogênicos/metabolismo , Ciclopentanos/farmacologia , Oxilipinas/farmacologia , Triterpenos Pentacíclicos/metabolismo , Senna (Planta)/efeitos dos fármacos , Senna (Planta)/metabolismo , Células A549 , Antineoplásicos Fitogênicos/biossíntese , Antineoplásicos Fitogênicos/farmacologia , Apoptose/efeitos dos fármacos , Reatores Biológicos , Biotecnologia , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Dano ao DNA , Fragmentação do DNA/efeitos dos fármacos , Humanos , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Triterpenos Pentacíclicos/farmacologia , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Reguladores de Crescimento de Plantas/farmacologia , Raízes de Plantas/efeitos dos fármacos , Raízes de Plantas/crescimento & desenvolvimento , Raízes de Plantas/metabolismo , Plantas Geneticamente Modificadas , Plantas Medicinais/efeitos dos fármacos , Plantas Medicinais/crescimento & desenvolvimento , Plantas Medicinais/metabolismo , Senna (Planta)/crescimento & desenvolvimento
14.
Chem Soc Rev ; 50(20): 11227-11248, 2021 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-34661214

RESUMO

Despite significant advances, the therapeutic impact of photodynamic therapy is still substantially hampered by the restricted penetration depth of light and the reactive oxygen species (ROS)-mediated toxicity, which is impeded by the shorter effective half-life and radius of ROS produced during treatment. Sonodynamic therapy (SDT), on the other hand, provides unrivalled benefits in deep-seated tumour ablation due to its deep penetration depth and not totally ROS-dependent toxicity, exhibiting enormous preclinical and clinical potential. In this tutorial review, we highlight imaging-guided precise SDT, which allows choosing the best treatment option and monitoring the therapy response in real-time, as well as recent clinical trials based on SDT. Aside from that, the subtle design strategies of sonosensitizers based on tumour environment shaping and rational structure modification, as well as SDT combination treatment (chemotherapy, chemodynamic therapy, photodynamic therapy, photothermal therapy, gas therapy and immunotherapy), aimed at a more effective treatment outcome, are summarized. Finally, we discussed the future of SDT for personalized cancer and other disease treatments.


Assuntos
Neoplasias , Fotoquimioterapia , Terapia por Ultrassom , Biotecnologia , Terapia Combinada , Humanos , Neoplasias/tratamento farmacológico
15.
IET Nanobiotechnol ; 15(4): 348-357, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-34694709

RESUMO

In the last decade, carbon-based nanostructures such as buckyball (C60 ), carbon nanotube (CNT), graphene and three-dimensional (3D) graphene have been identified as promising materials for electronic, electrochemical energy storage (batteries and supercapacitors), optical and sensing applications. Since the discovery of graphene in 2004, scientists have devised mass production techniques and explored graphene as a promising material for a wide range of applications. Most of the electronic and solar cell applications require materials with good electronic conductivity, mobility and finite bandgap. Graphene is a zero bandgap material which prevents it from the mainstream applications. On the other hand, 3D graphene has good electronic conductivity, mobility, bandgap and electrochemical properties. This review article will focus on the synthesis of the 3D graphene, its structure-property relationships, biotechnology and electronic applications and the hidden properties that are yet to be explored fully.


Assuntos
Grafite , Nanoestruturas , Nanotubos de Carbono , Biotecnologia , Eletrônica
16.
Molecules ; 26(20)2021 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-34684821

RESUMO

Currently, there is a growing demand for flavorings, especially of natural origin. It is worth paying attention to the biotechnological processes of flavor production, characterized by simplicity, high efficiency and relatively low cost. In this study, we analyzed the ability of the Galac tomyces geotrichum mold to transform by-products of the dairy industry: sour whey and buttermilk to complex flavour mixtures with pleasant, honey-rose aroma. Furthermore, the aroma complexity of the fermentation product has been carefully identified applying a sensomic approach involving the use of gas chromatography-olfactometry (GC-O), gas chromatography-mass spectrometry (GC-MS) and stable isotope dilution assay (SIDA) to identify and quantify aroma compounds. Based on the calculation of odor activity value (OAV), 13 key aroma compounds were present in both tested variants. The highest OAVs were found for phenylacetaldehyde (honey-like) in the buttermilk variant (912) and 2-phenylethanol (rose-like) in the sour whey variant (524). High values of this indicator were also recorded for phenylacetaldehyde (319) and 3-methyl-1-butanol with a fruity aroma (149) in the sour whey culture. The other compounds identified are 3-methylbutanal (malty), 2,3-butanedione (cheesy), isovaleric acid (cheesy), 3-(methylthio)-propanal (boiled potato), butanoic acid (vinegar), (E)-2-nonenal (fatty), ethyl furaneol (burnt sugar), dimethyl trisulfide (cabbage), and acetic acid (vinegar).


Assuntos
Leitelho/análise , Leitelho/microbiologia , Microbiologia de Alimentos , Geotrichum/metabolismo , Odorantes/análise , Soro do Leite/química , Soro do Leite/microbiologia , Acetaldeído/análogos & derivados , Acetaldeído/análise , Biotecnologia , Biotransformação , Fermentação , Aromatizantes/metabolismo , Tecnologia de Alimentos , Cromatografia Gasosa-Espectrometria de Massas , Humanos , Olfatometria , Álcool Feniletílico/análise , Paladar , Compostos Orgânicos Voláteis/análise
17.
Nanoscale ; 13(40): 16834-16846, 2021 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-34622910

RESUMO

DNA-based nanotechnology has evolved into an autonomous, highly innovative, and dynamic field of research at the nexus of supramolecular chemistry, nanotechnology, materials science, and biotechnology. DNA-based materials, including origami nanodevices, have started to emerge as an ideal scaffold for use in cellular programming, tissue engineering, and drug delivery applications. We cover herein the applications for DNA as a scaffold for interfacing with, and guiding, the activity of biological systems like cells and tissues. Although DNA is a highly programmable molecular building block, it suffers from a lack of functional capacity for guiding and modulating cells. Coupling DNA to biologically active molecules can bestow bioactivity to these nanodevices. The main goal of such nanodevices is to synthesize systems that can bind to cells and mimic the extracellular environment, and serve as a highly promising toolbox for multiple applications in cellular programming and tissue engineering. DNA-based programmable devices offer a highly promising approach for programming collections of cells, tissue engineering, and regenerative medicine applications.


Assuntos
DNA , Engenharia Tecidual , Biotecnologia , Nanotecnologia , Medicina Regenerativa
18.
Planta ; 254(5): 91, 2021 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-34617240

RESUMO

MAIN CONCLUSION: This work reviews recent advances in the pathways and key enzymes of steroidal saponins biosynthesis and sets the foundation for the biotechnological production of these useful compounds through transformation of microorganisms. Steroidal saponins, due to their specific chemical structures and active effects, have long been important natural products and that are irreplaceable in hormone production and other pharmaceutical industries. This article comprehensively reviewed the previous and current research progress and summarized the biosynthesis pathways and key biosynthetic enzymes of steroidal saponins that have been discovered in plants and microoganisms. On the basis of the general biosynthetic pathway in plants, it was found that the starting components, intermediates and catalysing enzymes were diverse between plants and microorganisms; however, the functions of their related enzymes tended to be similar. The biosynthesis pathways of steroidal saponins in microorganisms and marine organisms have not been revealed as clearly as those in plants and need further investigation. The elucidation of biosynthetic pathways and key enzymes is essential for understanding the synthetic mechanisms of these compounds and provides researchers with important information to further develop and implement the massive production of steroidal saponins by biotechnological approaches and methodologies.


Assuntos
Saponinas , Biotecnologia
19.
Cells ; 10(10)2021 09 24.
Artigo em Inglês | MEDLINE | ID: mdl-34685505

RESUMO

The objective of this review is to describe the evolution of lung tissue-derived diploid progenitor cell applications, ranging from historical biotechnological substrate functions for vaccine production and testing to current investigations around potential therapeutic use in respiratory tract regenerative medicine. Such cell types (e.g., MRC-5 or WI-38 sources) were extensively studied since the 1960s and have been continuously used over five decades as safe and sustainable industrial vaccine substrates. Recent research and development efforts around diploid progenitor lung cells (e.g., FE002-Lu or Walvax-2 sources) consist in qualification for potential use as optimal and renewed vaccine production substrates and, alternatively, for potential therapeutic applications in respiratory tract regenerative medicine. Potentially effective, safe, and sustainable cell therapy approaches for the management of inflammatory lung diseases or affections and related symptoms (e.g., COVID-19 patients and burn patient severe inhalation syndrome) using local homologous allogeneic cell-based or cell-derived product administrations are considered. Overall, lung tissue-derived progenitor cells isolated and produced under good manufacturing practices (GMP) may be used with high versatility. They can either act as key industrial platforms optimally conforming to specific pharmacopoeial requirements or as active pharmaceutical ingredients (API) for potentially effective promotion of lung tissue repair or regeneration.


Assuntos
Biotecnologia/métodos , Diploide , Pulmão/citologia , Medicina Regenerativa/métodos , Infecções Respiratórias/terapia , Animais , Bancos de Espécimes Biológicos , Vacinas contra COVID-19 , Linhagem Celular , Terapia Baseada em Transplante de Células e Tecidos , História do Século XX , História do Século XXI , Humanos , Pulmão/fisiologia , Regeneração , Medicina Regenerativa/história , SARS-CoV-2 , Transplante de Células-Tronco , Células-Tronco/citologia , Transplante Homólogo
20.
Biochem J ; 478(20): 3685-3721, 2021 10 29.
Artigo em Inglês | MEDLINE | ID: mdl-34673920

RESUMO

Optimising the function of a protein of length N amino acids by directed evolution involves navigating a 'search space' of possible sequences of some 20N. Optimising the expression levels of P proteins that materially affect host performance, each of which might also take 20 (logarithmically spaced) values, implies a similar search space of 20P. In this combinatorial sense, then, the problems of directed protein evolution and of host engineering are broadly equivalent. In practice, however, they have different means for avoiding the inevitable difficulties of implementation. The spare capacity exhibited in metabolic networks implies that host engineering may admit substantial increases in flux to targets of interest. Thus, we rehearse the relevant issues for those wishing to understand and exploit those modern genome-wide host engineering tools and thinking that have been designed and developed to optimise fluxes towards desirable products in biotechnological processes, with a focus on microbial systems. The aim throughput is 'making such biology predictable'. Strategies have been aimed at both transcription and translation, especially for regulatory processes that can affect multiple targets. However, because there is a limit on how much protein a cell can produce, increasing kcat in selected targets may be a better strategy than increasing protein expression levels for optimal host engineering.


Assuntos
Proteínas de Bactérias/genética , Evolução Molecular Direcionada/métodos , Proteínas Fúngicas/genética , Engenharia Metabólica/métodos , Engenharia de Proteínas/métodos , Proteínas de Bactérias/metabolismo , Biotecnologia/métodos , Epistasia Genética , Proteínas Fúngicas/metabolismo , Estudos de Associação Genética , Genoma Bacteriano , Genoma Fúngico , Microbiologia Industrial/métodos , Redes e Vias Metabólicas/genética , Metabolômica/métodos , Biossíntese de Proteínas , Proteômica/métodos , Transcrição Genética
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...