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1.
Metas enferm ; 25(9): 0676-0677, Nov. 2022. ilus
Artigo em Espanhol | IBECS | ID: ibc-213271

RESUMO

José Manuel Martín González trabaja en la consulta de acccesos venosos del Hospital Virgen del Rocío (Sevilla), un servicio que nació en 2017 y desde entonces atienden a un 80% más de pacientes. En un primer momento estaba pensada para pacientes oncológicos y hematológicos, pero se ha ampliado y en ella se atienden a todos aquellos que puedan presentar problemas o dificultades a la hora de instaurarle una vía para la administración de fármacos, o bien precisen terapia intravenosa de larga duración.(AU)


Assuntos
Humanos , Masculino , Enfermeiros , Enfermagem , Serviços de Enfermagem , Sangue , Análise Química do Sangue
2.
Washington, D.C.; OPS; 2022-08-10.
em Espanhol | PAHO-IRIS | ID: phr-56233

RESUMO

Los productos medicinales derivados del plasma tienen un papel muy importante en el tratamiento de la hemofilia, las enfermedades inmunitarias, las infecciones virales y otras alteraciones graves de la salud. Su importancia es tal que varios de estos productos están incluidos en la Lista Modelo de la Organización Mundial de la Salud (OMS) de Medicamentos Esenciales. Por tanto, es fundamental facilitar el acceso a estos productos en todos los países, sobre todo en los de ingresos medianos y bajos, donde el acceso es muy desigual. Esta orientación contiene recomendaciones para aumentar el suministro de los productos medicinales derivados del plasma mediante el fraccionamiento del plasma obtenido en el propio país. Su finalidad es proporcionar un marco estratégico para ayudar a los países a lograr el acceso universal a productos sanguíneos que sean seguros, eficaces y de calidad en el período 2020-2023.


Assuntos
Plasma , Sangue , Transfusão de Componentes Sanguíneos , Equipamentos e Provisões , Acesso a Medicamentos Essenciais e Tecnologias em Saúde
3.
Rev. Soc. Argent. Diabetes ; 56(2): 41-42, mayo - ago. 2022.
Artigo em Espanhol | LILACS, BINACIS | ID: biblio-1395820

RESUMO

La glucemia es una palabra de fácil definición, glucosa en sangre, pero deja de ser simple cuando quiere entenderse el significado de determinado resultado. El desafío es interpretar correctamente el valor hallado.


Assuntos
Glicemia , Sangue , Glucose
4.
J Spec Oper Med ; 22(3): 9-14, 2022 Sep 19.
Artigo em Inglês | MEDLINE | ID: mdl-35862850

RESUMO

BACKGROUND: Transfusion of whole blood (WB) is a lifesaving treatment that prolongs life until definitive surgical intervention can be performed; however, collecting WB is a time-consuming and resource-intensive process. Furthermore, it may be difficult to collect sufficient WB at the point of injury to treat critically wounded patients or multiple hemorrhaging casualties. This study is a follow-up to the proof-of-concept study on the effect of airdrop on WB. In addition, this study confirms the statistical significance for the plausibility of using airdrop to deliver WB to combat medics treating casualties in the pre-hospital setting when Food and Drug Administration (FDA)-approved cold-stored blood products are not available. METHODS: Forty-eight units of WB were collected and loaded into a blood cooler that was dropped from a fixed-wing aircraft under a Standard Airdrop Training Bundle (SATB) parachute or 68-in pilot chute. Twenty-four of these units were dropped from a C-145 aircraft, and 24 were dropped from a C-130 aircraft. A control group of 15 units of WB was storedin a blood cooler that was not dropped. Baseline and post-intervention laboratory tests were measured in both airdroppedand control units, including complete blood count; prothrombin time/partial thromboplastin time (PT/PTT); pH, lactate,potassium, bilirubin, glucose, fibrinogen, and lactate dehydrogenase (LDH) levels; and peripheral blood smears. RESULTS: The blood cooler, cooling packs, and all 48 WB units did notsustain any major damage from the airdrop. There was noevidence of hemolysis. Except for the one slightly damagedbag that was not sampled, all airdropped blood met parameters for transfusion per the Joint Trauma System Whole BloodTransfusion Clinical Practice Guideline and the Associationfor the Advancement of Blood and Biotherapies (AABB) Circular of Information for the Use of Human Blood and BloodComponents. CONCLUSIONS: Airdrop of fresh or stored WB in ablood cooler with a chute is a viable way of delivering bloodproducts to combat medics treating hemorrhaging patientsin the pre-hospital setting. This study also demonstrated theportability of this technique for multiple aircraft. The techniques evaluated in this study have the potential for utilizationin other austere settings such as wilderness medicine or humanitarian disasters where an acute need for WB delivery by airdrop is the only option.


Assuntos
Aeronaves , Sangue , Transfusão de Sangue , Hemorragia/terapia , Humanos , Medicina Militar
5.
J Virol ; 96(15): e0075122, 2022 08 10.
Artigo em Inglês | MEDLINE | ID: mdl-35867566

RESUMO

Lumpy skin disease virus (LSDV) is a poxvirus that causes severe systemic disease in cattle and is spread by mechanical arthropod-borne transmission. This study quantified the acquisition and retention of LSDV by four species of Diptera (Stomoxys calcitrans, Aedes aegypti, Culex quinquefasciatus, and Culicoides nubeculosus) from cutaneous lesions, normal skin, and blood from a clinically affected animal. The acquisition and retention of LSDV by Ae. aegypti from an artificial membrane feeding system was also examined. Mathematical models of the data were generated to identify the parameters which influence insect acquisition and retention of LSDV. For all four insect species, the probability of acquiring LSDV was substantially greater when feeding on a lesion compared with feeding on normal skin or blood from a clinically affected animal. After feeding on a skin lesion LSDV was retained on the proboscis for a similar length of time (around 9 days) for all four species and for a shorter time in the rest of the body, ranging from 2.2 to 6.4 days. Acquisition and retention of LSDV by Ae. aegypti after feeding on an artificial membrane feeding system that contained a high titer of LSDV was comparable to feeding on a skin lesion on a clinically affected animal, supporting the use of this laboratory model as a replacement for some animal studies. This work reveals that the cutaneous lesions of LSD provide the high-titer source required for acquisition of the virus by insects, thereby enabling the mechanical vector-borne transmission. IMPORTANCE Lumpy skin disease virus (LSDV) is a high consequence pathogen of cattle that is rapidly expanding its geographical boundaries into new regions such as Europe and Asia. This expansion is promoted by the mechanical transmission of the virus via hematogenous arthropods. This study quantifies the acquisition and retention of LSDV by four species of blood-feeding insects and reveals that the cutaneous lesions of LSD provide the high titer virus source necessary for virus acquisition by the insects. An artificial membrane feeding system containing a high titer of LSDV was shown to be comparable to a skin lesion on a clinically affected animal when used as a virus source. This promotes the use of these laboratory-based systems as replacements for some animal studies. Overall, this work advances our understanding of the mechanical vector-borne transmission of LSDV and provides evidence to support the design of more effective disease control programmes.


Assuntos
Sangue , Dípteros , Comportamento Alimentar , Insetos Vetores , Doença Nodular Cutânea , Vírus da Doença Nodular Cutânea , Aedes/anatomia & histologia , Aedes/virologia , Animais , Bovinos/virologia , Ceratopogonidae/anatomia & histologia , Ceratopogonidae/virologia , Culex/anatomia & histologia , Culex/virologia , Dípteros/anatomia & histologia , Dípteros/fisiologia , Dípteros/virologia , Insetos Vetores/anatomia & histologia , Insetos Vetores/fisiologia , Insetos Vetores/virologia , Doença Nodular Cutânea/virologia , Vírus da Doença Nodular Cutânea/isolamento & purificação , Vírus da Doença Nodular Cutânea/fisiologia , Membranas Artificiais , Muscidae/anatomia & histologia , Muscidae/virologia , Fatores de Tempo
7.
Acta Trop ; 233: 106543, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-35643185

RESUMO

Animal trypanosomosis, caused by Trypanozoon trypanosomes (Trypanosoma evansi and T. equiperdum), and Trypanosoma vivax, is endemic to South American countries and has a negative impact on the livestock industry. However, the risk factors for trypanosomosis in Paraguay remain unknown. This study aimed to determine the risk factors for equine trypanosomosis in Paraguay based on a PCR-based molecular survey and individual horse sampling data. In this study, 739 blood samples were collected from horses in 16 departments of Paraguay between August 2019 and November 2020. To elucidate the risk factors for trypanosome infection, the relationship between trypanosome infection status detected by PCR and the location, sex, age, breed of horses, and season of sample collection was analyzed. There were no significant differences in trypanosome prevalence in horses between the eastern and western regions, ages, or breeds of horses in Paraguay. Sex and season were identified as risk factors for trypanosome infection in horses in Paraguay in the current study. These results suggest that the rainy-summer season, when vectors increase in number and their blood-sucking activity, could be the most important risk factor for trypanosome infection in Paraguay horses. Preventive measures and treatments should be developed to address these factors.


Assuntos
Doenças dos Cavalos , Tripanossomíase , Animais , Sangue/parasitologia , Feminino , Doenças dos Cavalos/diagnóstico , Doenças dos Cavalos/epidemiologia , Cavalos , Masculino , Paraguai/epidemiologia , Reação em Cadeia da Polimerase/veterinária , Prevalência , Fatores de Risco , Trypanosoma/genética , Trypanosoma/isolamento & purificação , Tripanossomíase/diagnóstico , Tripanossomíase/epidemiologia , Tripanossomíase/veterinária
8.
Proc Natl Acad Sci U S A ; 119(26): e2117083119, 2022 06 28.
Artigo em Inglês | MEDLINE | ID: mdl-35737841

RESUMO

There are still significant knowledge gaps in understanding the intrusion and retention of exogeneous particles into the central nervous system (CNS). Here, we uncovered various exogeneous fine particles in human cerebrospinal fluids (CSFs) and identified the ambient environmental or occupational exposure sources of these particles, including commonly found particles (e.g., Fe- and Ca-containing ones) and other compositions that have not been reported previously (such as malayaite and anatase TiO2), by mapping their chemical and structural fingerprints. Furthermore, using mouse and in vitro models, we unveiled a possible translocation pathway of various inhaled fine particles from the lung to the brain through blood circulation (via dedicated biodistribution and mechanistic studies). Importantly, with the aid of isotope labeling, we obtained the retention kinetics of inhaled fine particles in mice, indicating a much slower clearance rate of localized exogenous particles from the brain than from other main metabolic organs. Collectively, our results provide a piece of evidence on the intrusion of exogeneous particles into the CNS and support the association between the inhalation of exogenous particles and their transport into the brain tissues. This work thus provides additional insights for the continued investigation of the adverse effects of air pollution on the brain.


Assuntos
Encéfalo , Pulmão , Material Particulado , Animais , Sangue , Encéfalo/metabolismo , Humanos , Pulmão/química , Pulmão/metabolismo , Camundongos , Tamanho da Partícula , Material Particulado/análise , Material Particulado/sangue , Material Particulado/química , Material Particulado/metabolismo , Distribuição Tecidual
9.
Nature ; 606(7913): 255-256, 2022 06.
Artigo em Inglês | MEDLINE | ID: mdl-35650394
10.
Proc Natl Acad Sci U S A ; 119(23): e2112385119, 2022 06 07.
Artigo em Inglês | MEDLINE | ID: mdl-35648836

RESUMO

Anopheline mosquitoes rely on their highly sensitive chemosensory apparatus to detect diverse chemical stimuli that drive the host-seeking and blood-feeding behaviors required to vector pathogens for malaria and other diseases. This process incorporates a variety of chemosensory receptors and transduction pathways. We used advanced in vivo gene-editing and -labeling approaches to localize and functionally characterize the ionotropic coreceptor AcIr76b in the malaria mosquito Anopheles coluzzii, where it impacts both olfactory and gustatory systems. AcIr76b has a broad expression pattern in female adult antennal grooved pegs, coeloconic sensilla, and T1 and T2 sensilla on the labellum, stylets, and tarsi, as well as the larval sensory peg. AcIr76b is colocalized with the Orco odorant receptor (OR) coreceptor in a subset of cells across the female antennae and labella. In contrast to Orco and Ir8a, chemosensory coreceptors that appear essential for the activity of their respective sets of chemosensory neurons in mosquitoes, AcIr76b−/− mutants maintain wild-type peripheral responses to volatile amines on the adult palps, labellum, and larval sensory cone. Interestingly, AcIr76b−/− mutants display significantly increased responses to amines in antennal grooved peg sensilla, while coeloconic sensilla reveal significant deficits in responses to several acids and amines. Behaviorally, AcIr76b mutants manifest significantly female-specific insemination deficits, and although AcIr76b−/− mutant females can locate, alight on, and probe artificial blood hosts, they are incapable of blood feeding successfully. Taken together, our findings reveal a multidimensional functionality of Ir76b in anopheline olfactory and gustatory pathways that directly impacts the vectorial capacity of these mosquitoes.


Assuntos
Anopheles , Comportamento Alimentar , Malária , Mosquitos Vetores , Receptores Ionotrópicos de Glutamato , Animais , Anopheles/genética , Anopheles/fisiologia , Sangue , Feminino , Edição de Genes , Malária/parasitologia , Malária/transmissão , Mosquitos Vetores/genética , Mosquitos Vetores/fisiologia , Receptores Ionotrópicos de Glutamato/genética , Receptores Ionotrópicos de Glutamato/fisiologia , Sensilas/fisiologia , Olfato
11.
J Virol ; 96(11): e0010922, 2022 06 08.
Artigo em Inglês | MEDLINE | ID: mdl-35575554

RESUMO

Anelloviruses (AVs) are commensal members of the human blood virome. Even though it was estimated that over 90% of the human population carries AVs, the dynamics of the AV virome ("anellome") are unknown. We investigated the dynamics of blood anellomes in two healthy people followed up for more than 30 years. Both subjects were positive for AVs in the majority of samples. Alphatorquevirus (torque teno virus [TTV]) was the most common genus in both subjects, followed by Betatorquevirus (torque teno minivirus [TTMV]) and Gammatorquevirus (torque teno midivirus [TTMDV]). Almost five times more lineages were found in subject 1 than in subject 2, and the anellomes differed phylogenetically. Both anellomes remained compositionally stable, and 9 out of 64 AV lineages were detected in over half of the time points. We confirmed the long-term and short-term persistence of 13 lineages by specific quantitative PCR (qPCR). AV lineages were detected in blood for over 30 years. Noticeable differences in anellome richness were found between the tested subjects, but both anellomes remained compositionally stable over time. These findings demonstrate that the human blood anellome is personal and that AV infection is chronic and potentially commensal. IMPORTANCE Knowledge of the persistence of AVs in humans is crucial to our understanding of the nature of AV infection (chronic or acute) and the role of AV in the host. We therefore investigated the dynamics of anellovirus infection in two healthy people followed up for 30 years. Our findings suggest that the human blood anellovirus virome (anellome) remains stable and personal for decades.


Assuntos
Anelloviridae , Sangue , Infecções por Vírus de DNA , Torque teno virus , Anelloviridae/classificação , Anelloviridae/genética , Sangue/virologia , DNA Viral , Humanos , Filogenia , Torque teno virus/genética , Viroma
12.
Retrovirology ; 19(1): 7, 2022 05 18.
Artigo em Inglês | MEDLINE | ID: mdl-35585539

RESUMO

BACKGROUND: The potential risk and association of bovine leukemia virus (BLV) with human remains controversial as it has been reported to be both positive and negative in human breast cancer and blood samples. Therefore, establishing the presence of BLV in comprehensive human clinical samples in different geographical locations is essential. RESULT: In this study, we examined the presence of BLV proviral DNA in human blood and breast cancer tissue specimens from Japan. PCR analysis of BLV provirus in 97 Japanese human blood samples and 23 breast cancer tissues showed negative result for all samples tested using long-fragment PCR and highly-sensitive short-fragment PCR amplification. No IgG and IgM antibodies were detected in any of the 97 human serum samples using BLV gp51 and p24 indirect ELISA test. Western blot analysis also showed negative result for IgG and IgM antibodies in all tested human serum samples. CONCLUSION: Our results indicate that Japanese human specimens including 97 human blood, 23 breast cancer tissues, and 97 serum samples were negative for BLV.


Assuntos
Anticorpos Antivirais , DNA Viral , Vírus da Leucemia Bovina , Provírus , Anticorpos Antivirais/isolamento & purificação , Sangue/virologia , Neoplasias da Mama/virologia , DNA Viral/isolamento & purificação , Feminino , Humanos , Imunoglobulina G/isolamento & purificação , Imunoglobulina M/isolamento & purificação , Japão , Vírus da Leucemia Bovina/genética , Vírus da Leucemia Bovina/imunologia , Provírus/genética
13.
Turkiye Parazitol Derg ; 46(2): 108-113, 2022 05 23.
Artigo em Inglês | MEDLINE | ID: mdl-35604187

RESUMO

Objective: To investigate intestinal and blood parasites in people who have a history of traveling abroad during the Coronavirus disease-2019 pandemic and returning to Turkey. Methods: In this study, 104 patients with gastrointestinal system and/or fever complaints who had traveled abroad during the pandemic period and returned to Turkey were included. Parasitic agents were investigated by taking blood and stool samples from the patients. Additionally, urine samples were obtained from patients with hematuria or dysuria with the suspicion of schistosomiasis. A direct microscopic examination, the Crypto-Giardia immunochromatographic test, and ELISA methods were used in the examination of the stool samples. In order to detect Plasmodium species, blood samples were examined by preparing both the rapid diagnostic test and thick drop and thin smear preparations. Results: One or more parasite species were detected in 38 (38.5%) of 104 patients included in the study. While intestinal parasites were detected in 16 (32%) of 50 patients who traveled to Iran and 16 (33.3%) of 48 patients who traveled to Northern Iraq, blood parasites were not found. Schistosoma mansoni was detected in all 5 of the patients with a history of traveling to Sudan. Plasmodium falciparum was detected in 1 patient who traveled to the African continent. Conclusion: It is vital to take precautions to prevent parasitic diseases, such as malaria and schistosomiasis, during travels to African countries. During travels to neighboring countries of Turkey, such as Northern Iraq and Iran, hygiene should be paid attention to, so as to prevent contracting intestinal parasitic diseases. In addition, it was concluded that people who plan to travel abroad should have information about the endemic parasitic diseases of the country that they are going to.


Assuntos
COVID-19 , Enteropatias Parasitárias , Parasitemia , Parasitos , Doença Relacionada a Viagens , Animais , Sangue/parasitologia , COVID-19/epidemiologia , Fezes/parasitologia , Humanos , Enteropatias Parasitárias/epidemiologia , Enteropatias Parasitárias/parasitologia , Pandemias , Parasitemia/epidemiologia , Parasitemia/parasitologia , Parasitos/isolamento & purificação , Plasmodium/isolamento & purificação , Turquia/epidemiologia , Urina/parasitologia
14.
Lima; Perú. Ministerio de Salud; May. 2022. 37 p. tab.
Monografia em Espanhol | MINSAPERÚ | ID: biblio-1370463

RESUMO

La guía contiene el procedimiento para la determinación de la hemoglobina mediante hemoglobinómetro portátil, que incluye la punción capilar, recojo de la muestra de sangre y materiales que se utilizaran para su correcta aplicación


Assuntos
Sangue , Heme , Métodos
15.
Acta Parasitol ; 67(2): 932-942, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35384598

RESUMO

INTRODUCTION: Phlebotomine sand flies (Diptera: Psychodidae) are known as the vector of diseases such as leishmaniasis, bartonellosis and viral diseases. The aim of this study is to detect the host feeding pattern of sand flies in the endemic areas for leishmaniasis in Turkey (Antalya, Kayseri) and Northern Cyprus (TRNC) as well as the presence of Leishmania DNA in the specimens. METHODS: One-hundred seventy-six blood-fed sand fly specimens were examined for blood meal analysis. A SYBR Green-PCR assay was performed with specific forward primers for each host and a universal reverse primer. Primers of human and goat were used together in multiplex PCR while goat and cow were studied separately. ITS-1 qPCR assay was also performed on both blood-fed and non-blood-fed females to detect Leishmania parasites. RESULTS: Blood sources could be detected in 69 out of 176 blood-fed sand fly specimens. The results of blood meal analysis showed that specimens were fed mostly on cows (22.2%) followed by humans (5.7%), goats (2.8%) and dogs (0.6%). Multiple feeding patterns were also detected as human + cow (3.4%), cow + goat (2.8%) and human + goat (1.7%). Five of the blood-fed specimens were Leishmania spp. positive: P. major s.l. (n = 1), P. tobbi (n = 2) were L. tropica positive from Antalya, P. simici was positive for L. infantum from Kayseri and P. papatasi (n = 1) was positive for L. major from Cyprus. Leishmania infection rates were determined as 3.79%, 1.69% and 2.63% among the blood-fed sand fly specimens in Antalya, Kayseri and TRNC, respectively. CONCLUSION: The SYBR-Green-based multiplex PCR assay is a cost-effective and promising tool for blood meal identification of wild-caught sand flies as well as other blood-sucking arthropods. Feeding patterns of important vector species detected in the present study show the high risk in these endemic areas. As a next step, to identify the blood source in a shorter time and to make the test more sensitive, development of this assay to probe-based and multiplex PCR will be also planned.


Assuntos
Sangue , DNA de Protozoário , Insetos Vetores , Leishmania , Leishmaniose , Psychodidae , Animais , Sangue/parasitologia , Bovinos , Chipre/epidemiologia , DNA/genética , DNA/isolamento & purificação , DNA de Protozoário/genética , DNA de Protozoário/isolamento & purificação , Cães , Doenças Endêmicas , Comportamento Alimentar , Feminino , Análise de Alimentos , Insetos Vetores/parasitologia , Insetos Vetores/fisiologia , Leishmania/genética , Leishmaniose/diagnóstico , Leishmaniose/epidemiologia , Leishmaniose/veterinária , Refeições , Phlebotomus/parasitologia , Phlebotomus/fisiologia , Psychodidae/parasitologia , Psychodidae/fisiologia , Reação em Cadeia da Polimerase em Tempo Real , Turquia/epidemiologia
16.
Rev. med. vet. zoot ; 69(1): 40-45, ene.-abr. 2022. tab
Artigo em Espanhol | LILACS, COLNAL | ID: biblio-1389166

RESUMO

RESUMEN Se analizaron los niveles séricos de creatina quinasa-MB (CK-MB) y lactato deshidrogenasa (LDH) en 10 perros diagnosticados con enfermedad valvular degenerativa y en seis perros clinicamente sanos, con el objetivo de evaluar si sus niveles séricos indican daño miocárdico. Las muestras de suero se analizaron mediante el método UV. Se utilizó la prueba de diferenciación de medias para determinar diferencias entre medias, y la prueba de correlación de Pearson para determinar si existe correlación entre los niveles séricos de ambas enzimas. Los valores de CK-MB y de LDH fueron significativamente diferentes entre los dos grupos de pacientes. Los niveles de CK-MB y LDH tuvieron correlación positiva, pero no significativa.


ABSTRACT Serum levels of creatine kinase-MB (CK-MB) and lactate dehydrogenase (LDH) were analyzed in 10 dogs diagnosed with degenerative valvular disease and in six clinically healthy dogs with the objective of evaluating whether their serum levels indicate myocardial damage. Serum samples were analyzed by UV method. The mean differentiation test was used to determine differences between means and the Pearson correlation test was performed to determine if there was a correlation between the serum levels of both enzymes. The CK-MB and LDH values were significantly different between the two groups of patients. The levels of CK-MB and LDH had a positive but not significant correlation.


Assuntos
Animais , Cães , Biomarcadores , Creatina Quinase , Cães , Cardiopatias , Isoenzimas , L-Lactato Desidrogenase , Raios Ultravioleta , Sangue , Ácido Pirúvico , Soro , Veias Jugulares
17.
ScientificWorldJournal ; 2022: 1426837, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35281746

RESUMO

In this work, we deal with unsteady magnetohydrodynamic allowed convection inflow of blood with a carbon nanotubes model; the single and multiwalled carbon nanotubes of human blood are used as a based fluid. Two numerical methods used to study this model are the weighted average finite difference method and the nonstandard compact finite difference method. The proportional Caputo hybrid operator has been used to fractionalize the proposed model. Stability analysis has been construed by a kind of John von Neumann stability analysis. Numerical results are presented in diverse graphs, which manifest that the method is successful in solving the proposed model.


Assuntos
Nanotubos de Carbono , Sangue , Humanos , Hidrodinâmica , Magnetismo , Modelos Estatísticos , Nanotubos de Carbono/estatística & dados numéricos , Reologia
18.
J Ethnopharmacol ; 290: 115078, 2022 May 23.
Artigo em Inglês | MEDLINE | ID: mdl-35157954

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Minthostachys verticillata (Griseb.) Epling (Lamiaceae) is a plant used in folk medicine for digestive or respiratory disorders. In addition, it is incorporated as condiment, in foods, as beverage flavoring or mate. The ethnopharmacological interest of M. verticillata resides in its essential oil (EO). Part of group has demonstrated the immunomodulatory ability of EO giving this oil a biological potential not known until that moment and conducted studies to evaluate their possible application in diseases of veterinary interest. However, the immunomodulatory effects of EO administered orally have not been fully characterized. AIM OF THE STUDY: This study evaluated the impact of EO oral administration on gastrointestinal and immune health through measurement of immunological and oxidative parameters in mice. MATERIAL AND METHODS: The EO was extracted from the leaves, slender stems and flowers of M. verticillata by hydrodistillation and chemical analyzed by gas chromatography-mass spectrometry (GC-MS). Prior to in vivo study, the cytotoxic effect of EO was determined using the human colon carcinoma Caco-2 cell line. For in vivo study, three groups of male Balb/c mice (n = 3) were orally administered with saline solution (control group) and EO (5 or 10 mg/kg/day) during 10 consecutive days. Subsequently, histological and hematological parameters, cytokines production, oxidative markers and CD4+ and CD8+ T cells were evaluated. RESULTS: The chemical analysis of EO revealed the presence of a high content of monoterpenes, being the main pulegone (76.12%) and menthone (14.28%). The EO oral administration improved mice growth performance and modulated systemic adaptive immune response by increasing in the total leukocyte number. A high percentage of CD4+ T cells were observed whereas the number of CD8+ T cells was not altered. EO did not alter the morpho-physiology of intestine and improved total antioxidant capacity by decreasing MDA concentrations. In addition, EO decreased the IL-6 levels and increased in the IL-4 and IL-10 concentrations. CONCLUSION: Results indicate that M. verticillata EO modulate inflammatory and oxidative parameters constituting a natural alternative which could be applied to improve gastrointestinal and immune functionality in animals.


Assuntos
Sistema Digestório/efeitos dos fármacos , Sistema Imunitário/efeitos dos fármacos , Lamiaceae , Óleos Voláteis/farmacologia , Animais , Sangue/efeitos dos fármacos , Linfócitos T CD4-Positivos/efeitos dos fármacos , Linfócitos T CD8-Positivos/efeitos dos fármacos , Linhagem Celular Tumoral , Citocinas/efeitos dos fármacos , Relação Dose-Resposta a Droga , Humanos , Interleucina-10/metabolismo , Interleucina-4/metabolismo , Interleucina-6/metabolismo , Masculino , Medicina Tradicional , Camundongos , Camundongos Endogâmicos BALB C , Monoterpenos/química , Monoterpenos/farmacologia , Estresse Oxidativo/efeitos dos fármacos
19.
Nat Commun ; 13(1): 761, 2022 02 09.
Artigo em Inglês | MEDLINE | ID: mdl-35140201

RESUMO

DNA methylation microarrays can be employed to interrogate cell-type composition in complex tissues. Here, we expand reference-based deconvolution of blood DNA methylation to include 12 leukocyte subtypes (neutrophils, eosinophils, basophils, monocytes, naïve and memory B cells, naïve and memory CD4 + and CD8 + T cells, natural killer, and T regulatory cells). Including derived variables, our method provides 56 immune profile variables. The IDOL (IDentifying Optimal Libraries) algorithm was used to identify libraries for deconvolution of DNA methylation data for current and previous platforms. The accuracy of deconvolution estimates obtained using our enhanced libraries was validated using artificial mixtures and whole-blood DNA methylation with known cellular composition from flow cytometry. We applied our libraries to deconvolve cancer, aging, and autoimmune disease datasets. In conclusion, these libraries enable a detailed representation of immune-cell profiles in blood using only DNA and facilitate a standardized, thorough investigation of immune profiles in human health and disease.


Assuntos
Sangue/imunologia , Metilação de DNA/imunologia , Algoritmos , Basófilos , Linfócitos T CD4-Positivos , Linfócitos T CD8-Positivos , Ilhas de CpG , Epigênese Genética , Citometria de Fluxo , Humanos , Contagem de Leucócitos , Monócitos , Neutrófilos , Análise de Sequência com Séries de Oligonucleotídeos
20.
Proc Natl Acad Sci U S A ; 119(8)2022 02 22.
Artigo em Inglês | MEDLINE | ID: mdl-35165177

RESUMO

Hydrogen peroxide (H2O2) plays a key role in environmental chemistry, biology, and medicine. H2O2 concentrations typically are 6 to 10 orders of magnitude lower than that of water, making its quantitative detection challenging. We demonstrate that optimized NMR spectroscopy allows direct, interference-free, quantitative measurements of H2O2 down to submicromolar levels in a wide range of fluids, ranging from exhaled breath and air condensate to rain, blood, urine, and saliva. NMR measurements confirm the previously reported spontaneous generation of H2O2 in microdroplets that form when condensing water vapor on a hydrophobic surface, which can interfere with atmospheric H2O2 measurements. Its antimicrobial activity and strong seasonal variation speculatively could be linked to the seasonality of respiratory viral diseases.


Assuntos
Peróxido de Hidrogênio/análise , Espectroscopia de Ressonância Magnética/métodos , Ar/análise , Sangue , Análise Química do Sangue , Líquidos Corporais/química , Expiração/fisiologia , Fezes/química , Humanos , Chuva/química , Saliva/química , Urina/química
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