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1.
Magn Reson Imaging ; 95: 110-117, 2023 01.
Artigo em Inglês | MEDLINE | ID: mdl-34506910

RESUMO

PURPOSE: To assess the clinical potential of a set of new diffusion parameters (D, ß, and µ) derived from fractional order calculus (FROC) diffusion model in predicting microvascular invasion (MVI) of hepatocellular carcinoma (HCC). MATERIALS AND METHODS: Between January 2019 to November 2020, a total of 63 patients with HCC were enrolled in this study. Diffusion-weighted images were acquired by using ten b-values (0-2000 s/mm2). The FROC model parameters including diffusion coefficient (D), fractional order parameter (ß), a microstructural quantity (µ) together with a conventional apparent diffusion coefficient (ADC) were calculated. Intraclass coefficients were calculated for assessing the agreement of parameters quantified by two radiologists. The differences of these values between the MVI-positive and MVI-negative HCC groups were compared by using independent sample t-test or the Mann-Whitney U test. Then the parameters showing significant differences between subgroups, including the ß and D, were integrated to develop a comprehensive predictive model via binary logistic regression. The diagnostic performance was evaluated by receiver operating characteristic (ROC) analysis. RESULTS: Among all the studied diffusion parameters, significant differences were found in D, ß, and ADC between the MVI-positive and MVI-negative groups. MVI-positive HCCs showed significantly higher ß values (0.65 ± 0.17 vs. 0.51 ± 0.13, P = 0.001), along with lower D values (0.84 ± 0.11 µm2/ms vs. 1.03 ± 0.13 µm2/ms, P < 0.001) and lower ADC values (1.38 ± 0.46 µm2/ms vs. 2.09 ± 0.70 µm2/ms, P < 0.001) than those of MVI-negative HCCs. According to the ROC analysis, the combination of D and ß demonstrated the largest area under the ROC curve (0.920) compared with individual parameters (D: 0.912; ß: 0.733; and ADC: 0.831) for differentiating MVI-positive from MVI-negative HCCs. CONCLUSIONS: The FROC parameters can be used as noninvasive quantitative imaging markers for preoperatively predicting the MVI status of HCCs.


Assuntos
Cálculos , Carcinoma Hepatocelular , Neoplasias Hepáticas , Humanos , Carcinoma Hepatocelular/diagnóstico por imagem , Carcinoma Hepatocelular/patologia , Neoplasias Hepáticas/diagnóstico por imagem , Neoplasias Hepáticas/patologia , Projetos Piloto , Imagem de Difusão por Ressonância Magnética/métodos , Estudos Retrospectivos
2.
BMC Gastroenterol ; 22(1): 539, 2022 Dec 23.
Artigo em Inglês | MEDLINE | ID: mdl-36564715

RESUMO

BACKGROUND: Endoscopic transpapillary gallbladder stenting (EGBS) is considered for patients with contraindications to early surgery for acute calculus cholecystitis. However, evidence regarding the long-term outcomes of EGBS is insufficient to date. The aim of the study was to evaluate the feasibility of EGBS as a bridge to or alternative to surgery when there are contraindications. METHODS: We reviewed the cases of patients who underwent EGBS using a novel spiral-shaped plastic stent for acute calculus cholecystitis between January 2011 and December 2019. We retrospectively evaluated the long-term outcomes of EGBS using a novel spiral-shaped plastic stent. RESULTS: Forty-nine patients were included. The clinical success rate of EGBS was 97%. After EGBS, 25 patients (surgery group) underwent elective cholecystectomy and 24 patients did not (follow-up group). In the surgery group, the median period from EGBS to surgery was 93 days. There was a single late adverse event with cholecystitis recurrence. In the follow-up group, the median follow-up period was 236 days. Late adverse events were observed in eight patients, including recurrence of cholecystitis (four patients), duodenal penetration by the distal stent end (two patients), and distal stent migration (two patient). In the follow-up group, the time to recurrence of biliary obstruction was 527 days. CONCLUSIONS: EGBS with a novel spiral-shaped plastic stent is safe and effective for long-term acute calculus cholecystitis. There is a possibility of EGBS to be a bridge to surgery and a surgical alternative for acute calculus cholecystitis in patients with contraindications to early cholecystectomy.


Assuntos
Cálculos , Colecistite Aguda , Colecistite , Humanos , Vesícula Biliar/cirurgia , Estudos Retrospectivos , Endoscopia do Sistema Digestório/efeitos adversos , Colecistite Aguda/cirurgia , Colecistite/etiologia , Drenagem/efeitos adversos , Stents , Plásticos
3.
Commun Biol ; 5(1): 1384, 2022 12 19.
Artigo em Inglês | MEDLINE | ID: mdl-36536113

RESUMO

Looking for a biological fingerprint relative to new aspects of the relationship between humans and natural environment during prehistoric times is challenging. Although many issues still need to be addressed in terms of authentication and identification, microparticles hidden in ancient dental calculus can provide interesting information for bridging this gap of knowledge. Here, we show evidence about the role of edible plants for the early Neolithic individuals in the central Apennines of the Italian peninsula and relative cultural landscape. Dental calculi from human and animal specimens exhumed at Grotta Mora Cavorso (Lazio), one of the largest prehistoric burial deposits, have returned an archaeobotanical record made up of several types of palaeoecological proxies. The organic fraction of this matrix was investigated by a multidisciplinary approach, whose novelty consisted in the application of next generation sequencing to ancient plant DNA fragments, specifically codifying for maturase K barcode gene. Panicoideae and Triticeae starches, together with genetic indicators of Rosaceae fruits, figs, and Lamiaceae herbs, suggested subsistence practices most likely still based on wild plant resources. On the other hand, pollen, and non-pollen palynomorphs allowed us to outline a general vegetational framework dominated by woodland patches alternated with meadows, where semi-permanent settlements could have been established.


Assuntos
Cálculos , Frutas , Humanos , Animais , Plantas Comestíveis , Pólen , Poaceae , Florestas , DNA Antigo
4.
Phys Life Rev ; 43: 271-272, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36343570
6.
Sci Rep ; 12(1): 18569, 2022 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-36329129

RESUMO

A genome's nucleotide composition can usually be summarized with (G)uanine + (C)ytosine (GC) or (A)denine + (T)hymine (AT) frequencies as GC% = 100% - AT%. Genomic AT/GC content has been linked to environment and selective processes in asexually reproducing organisms. A model is presented relating the evolution of genomic GC content over time to AT [Formula: see text] GC and GC [Formula: see text] AT mutation rates. By employing Itô calculus it is shown that if mutation rates are subject to random perturbations, that can vary over time, several implications follow. In particular, an extra Brownian motion term appears influencing genomic nucleotide variability; the greater the random perturbations the more genomic nucleotide variability. This can have several interpretations depending on the context. For instance, reducing the influence of the random perturbations on the AT/GC mutation rates and thus genomic nucleotide variability, to limit fitness decreasing and deleterious mutations, will likely suggest channeling of resources. On the other hand, increased genomic nucleotide diversity may be beneficial in variable environments. In asexually reproducing organisms, the Brownian motion term can be considered to be inversely reflective of the selective pressures an organism is subjected to at the molecular level. The presented model is a generalization of a previous model, limited to microbial symbionts, to all asexually reproducing, non-recombining organisms. Last, a connection between the presented model and the classical Luria-Delbrück mutation model is presented in an Itô calculus setting.


Assuntos
Cálculos , Genoma , Humanos , Composição de Bases , Genômica , Nucleotídeos , Evolução Molecular
7.
Medicine (Baltimore) ; 101(43): e31557, 2022 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-36316832

RESUMO

BACKGROUND: Many guidelines for nonsurgical treatment of pancreatolithiasis suggest little guidance for patients with pancreatolithiasis who do not have abdominal pain. Some patients with pancreatolithiasis whom we have treated nonsurgically with extracorporeal shock-wave lithotripsy did not have abdominal pain, and we describe one of them here. METHODS AND RESULTS: A 42-year-old man complaining of an 8-kg weight loss over 6 months was admitted to a nearby hospital, where fasting blood sugar and hemoglobin A1c values were 500 mg/dL and 11.8%. Computed tomography showed stones in the head of the pancreas and dilation of the main pancreatic duct. He was referred to our hospital to be considered for nonsurgical treatment of pancreatolithiasis. His height and weight were 160 cm and 52 kg (body mass index, 20.31). No tenderness or other abdominal findings were evident. After obtaining informed consent for nonsurgical treatment despite absence of abdominal pain, we performed extracorporeal shock wave lithotripsy. Computed tomography showed disappearance of stones from the pancreatic head. At discharge, his weight had increased to 62 kg and hemoglobin A1c was 6.8%, though antidiabetic medication has since become necessary. CONCLUSION: We believe that nonsurgical treatment of pancreatolithiasis was helpful for this patient, and could improve exocrine and endocrine function in other patients without abdominal pain.


Assuntos
Cálculos , Litotripsia , Pancreatopatias , Masculino , Humanos , Adulto , Pancreatopatias/cirurgia , Cálculos/etiologia , Ductos Pancreáticos , Litotripsia/métodos , Dor Abdominal/etiologia , Dor Abdominal/terapia
8.
PLoS One ; 17(10): e0275325, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36206258

RESUMO

Research into the experiences of students in mathematics with a marginalized sexual identity has remained largely underexplored, relegating these experiences and individuals to remain invisible. In this study, we leverage Queer Theory to address this gap in research by reporting on the experiences of Queer-spectrum undergraduate students (n = 2,454) enrolled in introductory mathematics courses (Precalculus, Calculus 1, and Calculus 2) across the United States. Drawing on student data (n = 24,327) from the Student Post-Secondary Instructional Practices Survey, we examine reported outcomes of math learning experiences and access to learning environments. Overall, within introductory math courses, sexual identity had a significant relationship with accessing learning environments and mathematical learning experiences. Asexual students were among those that reported higher levels of interactions with peers and instructors, a greater sense of community & participation, positive math affect, and were more likely to access learning environments external to the course yet anticipated receiving a lower course grade when compared to straight students. Bisexual students reported lower levels of interactions with instructors, a diminished sense of community & participation, math engagement, positive math affect, and were less likely to access learning environments compared to straight students. These findings suggest that Queer-spectrum students experience mathematical learning in a different manner than their straight peers and in relation to other Queer spectrum identities. Queer-spectrum students with a sexualized identity perceive that mathematics environments are not normative places to be a part of the community, resulting in negative dispositions, reduced interactions, and lower academic success. The impact of sexual identity, although small, contributes to negative indicators of positive mathematical experiences and suggests that the normative discourses in mathematics that are identity neutral, heteronormative, and aligned with binary thinking are differentially impacting Queer-spectrum students.


Assuntos
Cálculos , Minorias Sexuais e de Gênero , Identidade de Gênero , Humanos , Matemática , Estudantes
9.
Sci Rep ; 12(1): 17634, 2022 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-36271022

RESUMO

Bullet impacts are a ubiquitous form of damage to the built environment resulting from armed conflicts. Bullet impacts into stone buildings result in surficial cratering, fracturing, and changes to material properties, such as permeability and surface hardness. Controlled experiments into two different sedimentary stones were conducted to characterise surface damage and to investigate the relationship between the impact energy (a function of engagement distance) and crater volumes. Simplified geometries of crater volume using only depth and diameter measurements showed that the volume of a simple cone provides the best approximation (within 5%) to crater volume measured from photogrammetry models. This result suggests a quick and efficient method of estimating crater volumes during field assessments of damage. Impact energy has little consistent effect on crater volume over the engagement distances studied (100-400 m), but different target materials result in an order of magnitude variation in measured crater volumes. Bullet impacts in the experiments are similar in appearance to damage caused by hypervelocity experiments, but crater excavation is driven by momentum transfer to the target rather than a hemispherical shock wave. Therefore in contrast to predictions of impact scaling relationships for hypervelocity experiments, target material plays the dominant role in controlling damage, not projectile energy.


Assuntos
Cálculos , Meteoroides , Humanos , Fenômenos Físicos
10.
Pancreatology ; 22(8): 1120-1125, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36273991

RESUMO

BACKGROUNDS/OBJECTIVES: Patients with chronic pancreatitis may develop pancreatic duct stones that can obstruct outflow leading to ductal hypertension and pain. Both endoscopic retrograde pancreatography (ERP) with per-oral pancreatoscopy (POP) and intraductal lithotripsy and extracorporeal shock wave lithotripsy (ESWL) are feasible options to attempt ductal stone clearance. This study aims to compare POP-guided lithotripsy with ESWL in the management of refractory symptomatic main pancreatic duct stones. METHODS: This is an open-label, multi-center, parallel, randomized clinical trial. Patients with chronic pancreatitis and main pancreatic duct stones ≥5 mm who fail standard ERP methods for stone removal will be eligible for this study. In total, 150 subjects will be randomized 1:1 to either ESWL or POP. A maximum of 4 sessions of either ESWL or POP will be allowed in each arm, with crossover permitted thereafter. The primary outcome is complete stone clearance and secondary outcomes include quality of life, pain scores, number of interventions, and daily opiate requirements. CONCLUSIONS: This study aims to answer the question of which lithotripsy method is superior in removing refractory pancreatic duct stones while addressing the effects of lithotripsy on quality of life and pain in patients with chronic calcific pancreatitis (ClinicalTrials.gov NCT04115826).


Assuntos
Cálculos , Litotripsia , Pancreatopatias , Pancreatite Crônica , Humanos , Qualidade de Vida , Colangiopancreatografia Retrógrada Endoscópica/métodos , Resultado do Tratamento , Cálculos/terapia , Cálculos/complicações , Ductos Pancreáticos , Litotripsia/métodos , Pancreatite Crônica/complicações , Pancreatite Crônica/terapia , Pancreatopatias/complicações , Dor/complicações , Ensaios Clínicos Controlados Aleatórios como Assunto , Estudos Multicêntricos como Assunto
11.
Khirurgiia (Mosk) ; (10): 28-34, 2022.
Artigo em Russo | MEDLINE | ID: mdl-36223147

RESUMO

OBJECTIVE: To improve the results of treatment of obstructive jaundice by using of oral cholangioscopy. MATERIAL AND METHODS: There were 321 patients with obstructive jaundice between October 2020 and November 2021. Of these, cholangioscopy (SpyGlass video system) was used in 18 patients. Patients were divided into two groups: group 1 (n=9) - malignant biliary strictures; group 2 (n=9) - choledocholithiasis with large calculi (≥1.2 cm). At admission, all patients underwent laboratory and instrumental examination. In the first group, bilioduodenal stenting with plastic stents 7 and 10 Fr in diameter, 7 to 12 cm long or self-expanding nitinol stents 0.8-1.0 cm in diameter, 6 to 10 cm long was carried out. Patients with large calculi underwent targeted laser lithotripsy under endoscopic control until formation of 1-cm fragments. These fragments were removed using a lithoextraction balloon and Dormia basket. RESULTS: Cholangioscopy (SpyGlass system) was performed within 2 days after admission. In group 1, tumor tissue overgrowths were found during cholangioscopy. Five out of 9 (55.6%) patients underwent bilioduodenal stenting for adequate biliary drainage. Of these, 4 (44.5%) patients developed acute edematous pancreatitis on the first postoperative day. This complication regressed after 3-4 days under therapy. Four (44.5%) patients died from cancer-related multiple organ failure. In group 2, cholangioscopy effectively visualized the calculus and ensured its destruction by laser contact lithotripsy. Intraoperative and postoperative complications were not revealed in both groups. CONCLUSION: SpyGlass system is effective and safe for diagnosis and treatment in 100% of patients with extrahepatic biliary strictures and/or large calculi.


Assuntos
Sistema Biliar , Cálculos , Colestase , Doenças do Sistema Digestório , Icterícia Obstrutiva , Colangiopancreatografia Retrógrada Endoscópica/efeitos adversos , Colangiopancreatografia Retrógrada Endoscópica/métodos , Constrição Patológica , Endoscopia do Sistema Digestório/efeitos adversos , Endoscopia do Sistema Digestório/métodos , Humanos , Icterícia Obstrutiva/diagnóstico , Icterícia Obstrutiva/etiologia , Icterícia Obstrutiva/cirurgia , Plásticos
12.
PLoS One ; 17(10): e0275364, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36223401

RESUMO

A dynamical model linking stress, social support, and health has been recently proposed and numerically analyzed from a classical point of view of integer-order calculus. Although interesting observations have been obtained in this way, the present work conducts a fractional-order analysis of that model. Under a periodic forcing of an environmental stress variable, the perceived stress has been analyzed through bifurcation diagrams and two well-known metrics of entropy and complexity, such as spectral entropy and C0 complexity. The results obtained by numerical simulations have shown novel insights into how stress evolves with frequency and amplitude of the perturbation, as well as with initial conditions for the system variables. More precisely, it has been observed that stress can alternate between chaos, periodic oscillations, and stable behaviors as the fractional order varies. Moreover, the perturbation frequency has revealed a narrow interval for the chaotic oscillations, while its amplitude may present different values indicating a low sensitivity regarding chaos generation. Also, the perceived stress has been noted to be highly sensitive to initial conditions for the symptoms of stress-related ill-health and for the social support received from family and friends. This work opens new directions of research whereby fractional calculus might offer more insight into psychology, life sciences, mental disorders, and stress-free well-being.


Assuntos
Cálculos , Dinâmica não Linear , Entropia , Humanos , Apoio Social , Estresse Psicológico
13.
J Zoo Wildl Med ; 53(3): 578-582, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-36214243

RESUMO

This article summarizes urinary stone submissions from foxes in human care to the Minnesota Urolith Center over 40 years. A previous report documented the analysis of uroliths from foxes that were submitted between 1981 and 2007.13 New data compiled from 2008 to 2021 included an additional 38 stones submitted from foxes, totaling 65 fox urolith submissions from 1981 to 2021. Struvite and cystine uroliths were most common, with the remainder comprised of calcium phosphate, calcium oxalate, compound, mixed, or miscellaneous material. Most stones were submitted from male foxes. Seventy-two percent of the stones were urocystoliths, and from 2010 to 2021, most stones were diagnosed antemortem and removed surgically. More than half of the stones were submitted from red foxes (Vulpes vulpes) and fennec foxes (Vulpes zerda). Urolithiasis in foxes may be an underrecognized condition, and data from this study suggest that clinicians should consider routine urinalysis and diagnostic imaging as part of the preventive medicine program for fox species, especially red foxes and fennec foxes.


Assuntos
Cálculos , Doenças do Cão , Cálculos Urinários , Urolitíase , Animais , Oxalato de Cálcio , Fosfatos de Cálcio/análise , Cálculos/veterinária , Cistina , Cães , Humanos , Compostos de Magnésio/análise , Masculino , Minnesota/epidemiologia , Fosfatos/análise , Estruvita , Cálculos Urinários/veterinária , Urolitíase/diagnóstico , Urolitíase/epidemiologia , Urolitíase/veterinária
14.
Evid Based Dent ; 23(3): 104-105, 2022 09.
Artigo em Inglês | MEDLINE | ID: mdl-36151282

RESUMO

Data sources The review searched for articles via PubMed, Web of Science, Scopus, and Ovid. OpenGrey and the Brazilian Digital Library of Thesis and Dissertations (BDTD) were used to search for grey literature. As well as a manual searches of the reference lists from the included studies, a Google Scholar search was undertaken and the first 300 hits were screened. There was no restriction on date or language.Study selection Two authors screened initially 10% of the abstracts against the inclusion and exclusion criteria and then a calibration calculation was completed showing excellent agreement (kappa coefficient = 0.93). In phase one, abstracts were screened independently between the same two authors and discrepancies were discussed and resolved. In phase two, the full articles of those abstracts accepted were screened, as well as those whose abstracts were unavailable. Discrepancies were discussed with a third author for the final decision.Data extraction and synthesis Data extracted included: study characteristics (authors, year of publication, study design, country, sampling, the presence of a pilot study, aim, calibration and losses); population (age range and sex, sociodemographics and sample size); exposure (eligibility criteria and type and classification criteria of vision impairment); and outcome (prevalence and mean and standard deviation of oral health issues, such as dental plaque, gingivitis, calculus, periodontitis, oral hygiene [OH], dental caries and traumatic dental injuries [TDI]). Discrepancies were discussed with a third author for the final decision. In total, 15 studies were included in the systematic review and 12 in the meta-analysis. For the meta-analysis, heterogeneity was measured using I2 statistics.Results The meta-analysis found visually impaired children and adolescents had significantly higher levels of plaque (mean difference [MD] = 0.80; 95% confidence interval [CI] = 0.58-1.02; I2= 96%), gingival inflammation (MD = 0.69; 95% CI = 0.02-1.37; I2 = 100%), calculus (MD = 0.04; 95% CI = 0.03-0.06; I2 = 0%), OH indices (MD = 0.71; 95% CI = 0.24-1.18; I2 = 97%) and decayed missing filled surface (DMFS/dmfs) (MD = 0.90; 95% CI = 0.68-1.13; I2 = 26%) compared to sighted peers. TDI were statistically significantly higher (OR = 3.86; 95% CI = 2.63-5.68; I2 = 0%) in visually impaired children and adolescents. There was no significant difference in decayed missing filled teeth and DMFS/dmfs or caries.Conclusions Children and adolescents who are visually impaired are at greater risk of TDI and levels of dental plaque, gingival inflammation, calculus and DMFS and poorer OH. Furthermore, robust homogenous studies are required to strengthen any evidence of association between oral health outcomes and visual impairment in children and adolescents. Future research should also explore the directionality of these relationships.


Assuntos
Cálculos , Cárie Dentária , Placa Dentária , Gengivite , Adolescente , Criança , Humanos , Inflamação , Saúde Bucal , Projetos Piloto
15.
Int Urol Nephrol ; 54(12): 3097-3106, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36085346

RESUMO

PURPOSE: To investigate whether cystine crystal-induced production of reactive oxygen species (ROS) and activation of NLRP3 inflammasome contribute to cystine calculi formation. METHODS: Slc7a9-knockout rats were created as cystine calculi animal models. Kidney histological examination using TEM and immunohistochemistry were performed. The protein expression of NLRP3 and IL-1ß and the concentrations of oxidative stress markers such as ROS, MDA and H2O2 in kidney tissues were estimated. In parallel, HK-2 human renal proximal tubule cells were exposed to cystine crystals and NAC treatment. The protein and mRNA expression levels of NLRP3 were evaluated. Finally, cell apoptosis and cystine crystal adherence were also assessed. RESULTS: Activation of the NLRP3 inflammasome and marked elevations in MDA, H2O2 and ROS levels were observed both in vivo and in vitro. In particular, the protein and mRNA expression of NLRP3 was significantly increased by cystine crystals, but could be restored by an inhibitor of ROS. In addition, cell apoptosis and cystine crystal adherence were promoted by the NLRP3 inflammasome. The expression of CD44, OPN and HA in HK-2 cells was markedly increased by cystine crystals, but could be decreased by NLRP3 siRNA treatment. CONCLUSION: Notably, we found that the activation of NLRP3 by cystine crystal-induced ROS production was of major importance in the pathogenesis of cystine calculi formation.


Assuntos
Cálculos , Inflamassomos , Animais , Ratos , Cistina , Peróxido de Hidrogênio , Inflamassomos/metabolismo , Interleucina-1beta/metabolismo , Proteína 3 que Contém Domínio de Pirina da Família NLR/genética , Proteína 3 que Contém Domínio de Pirina da Família NLR/metabolismo , Espécies Reativas de Oxigênio/metabolismo , RNA Mensageiro , RNA Interferente Pequeno
16.
Urolithiasis ; 50(6): 679-684, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-35988091

RESUMO

Cystinuria is an autosomal metabolic disorder caused by mutations in the SLC3A1 and SLC7A9 genes, encoding the amino acid transporter proteins rBAT and b0,+AT, respectively. Based on the causative gene, cystinuria is classified into 3 types: type A (SLC3A1), type B (SLC7A9), and type AB (SLC3A1 and SLC7A9). Patients with cystinuria exhibit hyperexcretion of cystine and dibasic amino acids in the urine and develop cystine crystals due to its low solubility in the urine, often resulting in calculus formation. In this study, we present an inbred strain FVB/NJcl mice affected with cystinuria. In the affected mouse kidney, Slc7a9 expression was completely abolished because of a large sequence deletion in the promoter region of the Slc7a9 mutant allele. Slc7a9-deficient mice with FVB/NJcl genetic background developed cystine calculi in the bladder with high penetrance, as compared to the previously reported mouse models of cystinuria. This model may be useful to understand the determinants of crystal aggregation, affecting calculus formation.


Assuntos
Sistemas de Transporte de Aminoácidos Neutros , Diamino Aminoácidos , Cálculos , Cistinúria , Camundongos , Animais , Cistinúria/genética , Cistinúria/metabolismo , Sistemas de Transporte de Aminoácidos Básicos/genética , Cistina/metabolismo , Mutação , Modelos Animais de Doenças , Diamino Aminoácidos/genética , Sistemas de Transporte de Aminoácidos Neutros/genética
17.
Magn Reson Imaging ; 93: 181-188, 2022 11.
Artigo em Inglês | MEDLINE | ID: mdl-35988835

RESUMO

OBJECTIVE: This study aimed to evaluate the value of a fractional order calculus (FROC) model combined with conventional magnetic resonance imaging (MRI) for differentiating cervical adenocarcinoma (CAC) from squamous cell carcinoma (SCC). METHODS: Diffusion-weighted imaging (DWI) with 9 b values (0-2000s/mm2) was carried out in 57 cervical cancer patients. Diffusion coefficient (D), fractional order parameter (ß), and microstructural quantity (µ) together with apparent diffusion coefficient (ADC) were calculated and compared between the CAC and SCC groups. Conventional MRI features included T2WI signal intensity (SI), unenhanced-T1WI SI, enhanced-T1WI SI, and ∆T1WI SI, which were also compared between the two groups. Receiver operating characteristic (ROC) analysis was employed to assess the performance of FROC parameters, ADC, and conventional MRI features in differentiating CAC from SCC. RESULTS: ß was significantly lower in the CAC group than in the SCC group (0.682 ± 0.054 vs. 0.723 ± 0.084, P = 0.035), while D and µ were not significantly different between the two groups (D, P = 0.171; µ, P = 0.127). There was no significant difference in the ADC value between the two groups (P = 0.053). In conventional MRI features, enhanced-T1WI SI was significantly higher in the SCC group than in the CAC group (985.78 ± 130.83 vs. 853.92 ± 149.65, P = 0.002). The area under the curve (AUC) of ß, ADC, and enhanced-T1WI SI was 0.700, 0.683, and 0.799, respectively. The combination of ß, ADC, and enhanced-T1WI SI revealed optimal diagnostic performance in differentiating CAC from SCC (AUC = 0.930), followed by ß + enhanced-T1WI SI (AUC = 0.869), ADC+ enhanced-T1WI SI (AUC = 0.817), and ß + ADC (AUC = 0.761). CONCLUSION: The FROC model can serve as a noninvasive and quantitative imaging technique for differentiating CAC from SCC. ß combined with ADC and enhanced-T1WI SI had the highest diagnostic efficiency.


Assuntos
Cálculos , Neuroblastoma , Neoplasias do Colo do Útero , Imagem de Difusão por Ressonância Magnética/métodos , Feminino , Humanos , Imageamento por Ressonância Magnética , Curva ROC , Neoplasias do Colo do Útero/diagnóstico por imagem
19.
Medicine (Baltimore) ; 101(32): e30063, 2022 Aug 12.
Artigo em Inglês | MEDLINE | ID: mdl-35960111

RESUMO

BACKGROUND: Pancreatic extracorporeal shock wave lithotripsy (P-ESWL) is recommended as the first-line treatment for large pancreatic stones. While complications such as post-P-ESWL pancreatitis, bleeding, infection, steinstrasse, and perforation have been reported in the past 30 years, lung contusion has never been reported. The present case demonstrates lung contusion as a complication after P-ESWL. METHODS: A 48-year-old man was admitted to our department due to painful chronic pancreatitis with pancreatic duct stones. Computed tomography revealed normal lungs. P-ESWL was performed. The shock wave head contacted with right upper quadrant and the path of shock wave was at a 45° angle to the ventral midline. After P-ESWL, multiple patchy high-density shadows in the lower lobe of right lung were found, which was normal before P-ESWL. The patient had no symptoms of lung injury. RESULTS AND CONCLUSION: Laboratory studies revealed elevated D-dimer from 0.33 to 0.74 ug/mL, which was consistent with abnormal clotting of lung contusion. Chest computed tomography showed slight pleural effusion. Considering the interval between 2 X-rays was only 3 hours, we inferred that lung contusion was related to P-ESWL. The patient displayed stable vital signs, therefore, no specific interventions were conducted. Three days after P-ESWL, endoscopic retrograde cholangiopancreatography was performed and the lung shadows were partially absorbed. Considering the location of shock wave head, it was possible to cause lung contusion in lower lobe of right lung. More than 10,000 P-ESWL therapeutic sessions had been performed in our center since 2010, and it is the first case about lung contusion as a complication. It is also the first report to describe lung contusion after P-ESWL. Although the patient was asymptomatic, it should raise awareness of clinicians.


Assuntos
Cálculos , Contusões , Litotripsia , Lesão Pulmonar , Colangiopancreatografia Retrógrada Endoscópica/métodos , Contusões/complicações , Contusões/terapia , Humanos , Litotripsia/efeitos adversos , Litotripsia/métodos , Pulmão , Lesão Pulmonar/complicações , Lesão Pulmonar/etiologia , Masculino , Pessoa de Meia-Idade , Ductos Pancreáticos , Resultado do Tratamento
20.
J Vet Sci ; 23(5): e65, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-36038186

RESUMO

BACKGROUND: Persistent uroliths after a cystotomy in dogs are a common cause of surgical failure. OBJECTIVES: This study examined the following: the success rate of retrograde urohydropropulsion in male dogs using non-enhanced computed tomography (CT), whether the CT mean beam attenuation values in Hounsfield Units (mHU) measured in vivo could predict the urolithiasis composition and whether the selected reconstruction kernel may influence the measured mHU. METHODS: All dogs and cats that presented with lower urinary tract uroliths and had a non-enhanced CT preceding surgery were included. In male dogs, CT was performed after retrograde urohydropropulsion to detect the remaining urethral calculi. The percentage and location of persistent calculi were recorded. The images were reconstructed using three kernels, from smooth to ultrasharp, and the calculi mHU were measured. RESULTS: Sixty-five patients were included in the study. The success rate of retrograde urohydropropulsion in the 45 male dogs was 55.6% and 86.7% at the first and second attempts, respectively. The predominant components of the calculi were cystine (20), struvite (15), calcium oxalate (8), and urate (7). The convolution kernel influenced the mHU values (p < 0.05). The difference in mHU regarding the calculus composition was better assessed using the smoother kernel. A mHU greater than 1,000 HU was predictive of calcium oxalate calculi. CONCLUSIONS: Non-enhanced CT is useful for controlling the success of retrograde urohydropropulsion. The mHU could allow a prediction of the calculus composition, particularly for calcium oxalate, which may help determine the therapeutic strategy.


Assuntos
Cálculos , Doenças do Gato , Doenças do Cão , Cálculos Urinários , Animais , Oxalato de Cálcio , Cálculos/veterinária , Doenças do Gato/cirurgia , Gatos , Cistina , Doenças do Cão/diagnóstico por imagem , Doenças do Cão/cirurgia , Cães , Masculino , Estruvita , Tomografia Computadorizada por Raios X/veterinária , Ácido Úrico , Cálculos Urinários/diagnóstico por imagem , Cálculos Urinários/veterinária
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