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Int J Biol Macromol ; 188: 369-374, 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-34371044


Sodium channels selectively conduct Na+ ions across cellular membrane with extraordinary efficiency, which is essential for initiating action potentials. However, how Na+ ions permeate the ionic channels remains obscure and ambiguous. With more than 40 conductance events from microsecond molecular dynamics simulation, the soft knock-on ion permeation mediated by water molecules was observed and confirmed by the free energy profile and electrostatic potential calculation in this study. During the soft knock-on process, the change of average distance between four oxygen atoms in Glu177-Glu177 plays a very important role for the permeation of Na+ ion. Exploration of the ionic conductance mechanism could provide a guideline for designing ion channel targeted drug.

Campylobacterales/química , Transporte de Íons/genética , Sódio/química , Canais de Sódio Disparados por Voltagem/química , Proteínas de Bactérias/química , Proteínas de Bactérias/ultraestrutura , Campylobacterales/ultraestrutura , Glutamina/química , Simulação de Dinâmica Molecular , Oxigênio/química , Conformação Proteica , Eletricidade Estática , Água/química
Sci Rep ; 10(1): 10023, 2020 06 22.
Artigo em Inglês | MEDLINE | ID: mdl-32572050


The intensification of marine aquaculture raises multiple sustainability issues, namely the handling of nutrient-rich effluents that can adversely impact ecosystems. As integrated multi-trophic aquaculture (IMTA) gains momentum, the use of halophyte plants to phytoremediate aquaculture effluents has received growing attention, particularly in aquaponics. It is, therefore, important to obtain a more in-depth knowledge of the microbial communities present in the root systems of these plants, both in their natural environment (sediment) and in aquaponics, in order to understand their nutrient removal potential. The present study used denaturing gradient gel electrophoresis (DGGE) and barcoded pyrosequencing to assess the bacterial community present in the endosphere and rhizosphere of three halophyte plants: Halimione portulacoides, Salicornia ramosissima and Sarcocornia perennis. Species-specific effects were recorded in the profile and diversity of the bacterial communities present in halophyte roots, with significant differences also recorded for the same halophyte species grown in contrasting environments (sediment vs. aquaponics). In aquaponics the most abundant groups belonged to the orders Rhodocyclales, Campylobacterales, Rhodobacterales and Desulfobacterales, while in the natural environment (sediment) the most abundant groups belonged to the orders Rhizobiales, Sphingomonadales and Alteromonadales. An overall enrichment in bacterial taxa involved in nutrient cycling was recorded in the roots of halophytes grown in aquaponics (such as Denitromonas, Mesorhizobium, Colwellia, Dokdonella and Arcobacter), thereby highlighting their potential to reduce the nutrient loads from aquaculture effluents.

Aquicultura/métodos , Raízes de Plantas/microbiologia , Rizosfera , Plantas Tolerantes a Sal/microbiologia , Microbiologia do Solo , Gerenciamento de Resíduos/métodos , Animais , Campylobacterales/metabolismo , Chenopodiaceae/crescimento & desenvolvimento , Chenopodiaceae/microbiologia , Eletroforese em Gel de Gradiente Desnaturante , Peixes , Sequenciamento de Nucleotídeos em Larga Escala , Microbiota/genética , RNA Ribossômico 16S/genética , Plantas Tolerantes a Sal/fisiologia
J Med Food ; 23(2): 114-124, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31747353


As the functions of Lactobacilli become better understood, there are increasing numbers of applications for Lactobacillus products. Previously, we have demonstrated that Lactobacillus rhamnosus GG (LGG) can prevent alcoholic liver injury. LGG granules were produced by fluid bed granulation with a media composed of starch, skimmed milk powder, whey powder, microcrystalline cellulose and maltose, and LGG fermented liquid that comprised 30-50% of the total weight. We found LGG granules dose-dependently protected against chronic alcoholic liver disease. When alcohol was consumed for 8 weeks with LGG treatment during the last 2 weeks, we demonstrated that the dose dependence of LGG granules can improve alcohol-induced liver injury through decreasing the levels of lipopolysaccharide and tumor necrosis factor-α in serum and prevent liver steatosis by suppressing triglyceride, free fatty acid, and malondialdehyde production in liver. Alcohol feeding caused a decline in the number of both Lactobacillus and Bifidobacterium, with a proportional increase in the number of Clostridium perfringens in ileum, and expansion of the Gram-negative bacteria Proteobacteria, Campylobacterales, and Helicobacter in cecum. However, LGG granule treatment restored the content of these microorganisms. In conclusion, LGG granule supplementation can improve the intestinal microbiota, reduce the number of gram-negative bacteria, and ameliorate alcoholic liver injury.

Doença Hepática Crônica Induzida por Substâncias e Drogas/microbiologia , Doença Hepática Crônica Induzida por Substâncias e Drogas/terapia , Microbioma Gastrointestinal/fisiologia , Intestinos/microbiologia , Lacticaseibacillus rhamnosus/fisiologia , Probióticos/uso terapêutico , Alanina Transaminase/sangue , Animais , Aspartato Aminotransferases/sangue , Bifidobacterium/crescimento & desenvolvimento , Campylobacterales/crescimento & desenvolvimento , Clostridium perfringens/crescimento & desenvolvimento , Microbioma Gastrointestinal/genética , Helicobacter/crescimento & desenvolvimento , Íleo/microbiologia , Lactobacillus/crescimento & desenvolvimento , Masculino , Malondialdeído/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Proteobactérias/crescimento & desenvolvimento , RNA Ribossômico 16S/genética , RNA Ribossômico 16S/isolamento & purificação , Triglicerídeos/sangue , Fator de Necrose Tumoral alfa/sangue