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1.
Artigo em Inglês | MEDLINE | ID: mdl-34339956

RESUMO

Ylang-ylang (YY) essential oil (EO) is distilled from the fresh-mature flowers of the Annonaceae family tropical tree Cananga odorata [Lam.] Hook. f. & Thomson, and is widely used in perfume and cosmetic industries for its fragrant character. Herein, two different metabolomic profiles obtained using high-performance thin-layer chromatography (HPTLC), applying different stains, namely 2,2-diphenyl-1-picrylhydrazyl (DPPH·) and p-anisaldehyde, were used for discrimination of 52 YY samples across geographical origins and distillation grades. The first profile is developed using the DPPH· stain based on the radical scavenging activity (RSA) of YY EOs. Results of the HPTLC-DPPH· assay confirmed that RSA of YY EOs is in proportion to the length of distillation times. Major components contributing to the RSA of YY EOs were tentatively identified as germacrene D and α-farnesene, eugenol and linalool, by gas chromatography-mass spectrometry (GC-MS) and GC-flame ionisation detector (GC-FID). The second profile was developed using the general-purpose p-anisaldehyde stain based on the general chemical composition of YY EOs. Untargeted metabolomic discrimination of YY EOs from different geographical origins was performed based on the HPTLC-p-anisaldehyde profiles, followed by principal component analysis (PCA). A discrimination and prediction model for identification of YY distillation grade was developed using PCA and partial least squares regression (PLS) based on binned HPTLC-ultraviolet (254 nm) profiles, which was successfully applied to distillation grade determination of blended YY Complete EOs.


Assuntos
Cananga/química , Cromatografia em Camada Delgada/métodos , Sequestradores de Radicais Livres/química , Óleos Voláteis/química , Óleos Vegetais/química , Compostos de Bifenilo/análise , Compostos de Bifenilo/metabolismo , Cromatografia Líquida de Alta Pressão , Destilação , Eugenol/análise , Eugenol/química , Eugenol/metabolismo , Sequestradores de Radicais Livres/metabolismo , Metabolômica , Análise Multivariada , Óleos Voláteis/metabolismo , Picratos/análise , Picratos/metabolismo , Óleos Vegetais/metabolismo , Sesquiterpenos/análise , Sesquiterpenos/química , Sesquiterpenos/metabolismo
2.
Biomed Res Int ; 2021: 6697973, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34327237

RESUMO

Emerging drug-resistant bacteria creates an urgent need to search for antibiotics drugs with novel mechanisms of action. Endophytes have established a reputation as a source of structurally novel secondary metabolites with a wide range of biological activities. In the present study, we explore the antibacterial potential of endophytic fungi isolated from different tissues of Terminalia mantaly, Terminalia catappa, and Cananga odorata. The crude ethyl acetate extracts of 56 different endophytic fungi were screened against seven bacterial strains using the broth microdilution method. The antibacterial modes of action of the most active extracts (04) were evaluated using E. coli ATCC 25922 and H. influenzae ATCC 49247 strains. Both the DPPH and FRAP assays were used to investigate their antioxidant activity, and their cytotoxicity against the Vero cell line was evaluated using the MTT assay. Out of the 56 crude extracts tested, about 13% were considered very active, 66% partially active, and 21% nonactive against all tested bacterial strains with MIC values ranging from 0.32 µg/mL to 25 µg/mL. The four more potent extracts (MIC <5 µg/mL) (from Aspergillus sp. N454, Aspergillus sp. N13, Curvularia sp. N101, and Aspergillus sp. N18) significantly lysed the bacteria cells, increased outer membrane permeability, reduced salt tolerance, and inhibited bacterial catalase activity. They exhibited a DPPH free radical scavenging activity with IC50 ranging from 150.71 to 936.08 µg/mL. Three of the four potent extracts were noncytotoxic against the Vero cells line (CC50 > 100 µg/mL). Results from this investigation demonstrated that endophytes from Cameroonian medicinal plants might content potent antibacterial metabolites. The bioguided fractionation of these potent extracts is ongoing to isolate and characterise potential active ingredients.


Assuntos
Antibacterianos/farmacologia , Cananga/microbiologia , Endófitos/química , Fungos/química , Terminalia/microbiologia , Antioxidantes/farmacologia , Bactérias/efeitos dos fármacos , Compostos de Bifenilo/química , Catalase/metabolismo , Morte Celular/efeitos dos fármacos , Membrana Celular/efeitos dos fármacos , Permeabilidade da Membrana Celular/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Nucleotídeos/metabolismo , Picratos/química , Tolerância ao Sal/efeitos dos fármacos
3.
Int J Biol Macromol ; 171: 480-490, 2021 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-33428956

RESUMO

In this study, a comparative efficacy of Cananga odorata EO (CoEO) and its nanoencapsulated formulation into chitosan nanoemulsion (CoEO-CsNe) against a toxigenic strain of Aspergillus flavus (AF-M-K5) were investigated for the first time in order to determine its efficacy in preservation of stored food from fungal, aflatoxin B1 (AFB1) contamination and lipid peroxidation. GC and GC-MS analysis of CoEO revealed the presence of linalool (24.56%) and benzyl acetate (22.43%) as the major components. CoEO was encapsulated into chitosan nanoemulsion (CsNe) through ionic-gelation technique and characterized by High Resolution-Scanning Electron Microscopy (HR-SEM), Fourier Transform Infrared spectroscopy (FTIR), and X-Ray Diffraction (XRD) analysis. The CoEO-CsNe during in vitro investigation against A. flavus completely inhibited the growth and AFB1 production at 1.0 µL/mL and 0.75 µL/mL, respectively. Additionally, CoEO-CsNe showed improved antioxidant activity against DPPH• and ABTS•+ with IC50 value 0.93 and 0.72 µL/mL, respectively. Further, CoEO-CsNe suppressed fungal growth, AFB1 secretion and lipid peroxidation in Arachis hypogea L. during in situ investigation without causing any adverse effect on seed germination. Overall results demonstrated that the CoEO-CsNe has potential of being utilized as a suitable plant based antifungal agent to improve the shelf-life of stored food against AFB1 and lipid peroxidation mediated biodeterioration.


Assuntos
Antifúngicos/administração & dosagem , Antioxidantes/administração & dosagem , Arachis/microbiologia , Aspergillus flavus/efeitos dos fármacos , Cananga/química , Conservantes de Alimentos/administração & dosagem , Nanocápsulas/administração & dosagem , Óleos Voláteis/administração & dosagem , Óleos Vegetais/administração & dosagem , Aflatoxina B1/metabolismo , Antifúngicos/farmacologia , Antioxidantes/farmacologia , Aspergillus flavus/metabolismo , Avaliação Pré-Clínica de Medicamentos , Emulsões , Conservantes de Alimentos/farmacologia , Cromatografia Gasosa-Espectrometria de Massas , Germinação/efeitos dos fármacos , Química Verde , Concentração Inibidora 50 , Peroxidação de Lipídeos/efeitos dos fármacos , Microscopia Eletrônica de Varredura , Óleos Voláteis/farmacologia , Óleos Vegetais/farmacologia , Sementes/efeitos dos fármacos , Espectroscopia de Infravermelho com Transformada de Fourier , Difração de Raios X
4.
J Int Med Res ; 48(9): 300060520946237, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32883150

RESUMO

OBJECTIVES: To assess understudied, alternative suicide prevention modalities in a mental health care setting. METHODS: This was a prospective study of patients (n = 140, 68 cases and 72 controls) who were admitted to hospital or who contacted an SOS suicide crisis line for suicidal ideation or attempts. Psychiatric diagnoses (Mini-International Neuropsychiatric Interview) and intensity of anxiety/depression/suicidality (Hamilton Anxiety Rating Scale, Montgomery-Åsberg Depression Rating Scale, and Beck Scale for Suicidal Ideation) were assessed. All intervention group subjects received a crisis card with a crisis line number, interviews with psychologists or volunteers and a telephone call on days 10 to 21, then 6 months later. These subjects also had a choice between two further 4-month interventions: body contact care or mobile intervention team visits. RESULTS: The interventions significantly reduced the number of suicide attempts and suicide (3%) at 6 months compared with the control condition (12%). There were fewer losses to follow-up in the intervention group (7.35%) than in the control group (9.72%). CONCLUSIONS: The results favour the implementation of integrated care and maintaining contact in suicide prevention.


Assuntos
Aromaterapia , Cananga , Humanos , Projetos Piloto , Estudos Prospectivos , Tentativa de Suicídio
5.
Metab Eng ; 61: 397-405, 2020 09.
Artigo em Inglês | MEDLINE | ID: mdl-32795613

RESUMO

Multi-substrate terpene synthases (TPSs) are distinct from typical TPSs that react with a single substrate. Although in vitro activity of few multi-substrate TPSs have been reported, in vivo characterization has not been well investigated for most of them. Here, a new TPS from Cananga odorata, CoTPS5, belonging to TPS-f subfamily was functionally characterized in vitro as well as in vivo. CoTPS5 reacted with multiple prenyl-pyrophosphate substrates of various chain lengths as a multi-substrate TPS. It catalyzed the formation of (E)-ß-ocimene, (E,E)-α-farnesene and α-springene from geranyl pyrophosphate, (E,E)-farnesyl pyrophosphate and geranylgeranyl pyrophosphate, respectively. Upon transient expression in Nicotiana benthamiana, CoTPS5 localized to cytosol and produced only (E,E)-α-farnesene. However, expression of plastid-targeted CoTPS5 in N. benthamiana resulted in biosynthesis of all three compounds, (E)-ß-ocimene, (E,E)-α-farnesene and α-springene. Similarly, transgenic Arabidopsis plants overexpressing plastid-targeted CoTPS5 showed stable and sustainable production of (E)-ß-ocimene, (E,E)-α-farnesene and α-springene. Moreover, their production did not affect the growth and development of transgenic Arabidopsis plants. Our results demonstrate that redirecting multi-substrate TPS to a different intracellular compartment could be an effective way to prove in vivo activity of multi-substrate TPSs and thereby allowing for the production of multiple terpenoids simultaneously in plants.


Assuntos
Alquil e Aril Transferases , Arabidopsis , Cananga/genética , Proteínas de Plantas , Plantas Geneticamente Modificadas , Terpenos/metabolismo , Tabaco , Alquil e Aril Transferases/genética , Alquil e Aril Transferases/metabolismo , Arabidopsis/genética , Arabidopsis/metabolismo , Cananga/enzimologia , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas/genética , Plantas Geneticamente Modificadas/metabolismo , Tabaco/genética , Tabaco/metabolismo
6.
Int J Mol Sci ; 21(17)2020 Aug 26.
Artigo em Inglês | MEDLINE | ID: mdl-32859057

RESUMO

Spearmint produces and stores large amounts of monoterpenes, mainly limonene and carvone, in glandular trichomes and is the major natural source of these compounds. Towards producing heterologous monoterpenes in spearmint, we first reduced the flux into the native limonene pathway by knocking down the expression of limonene synthase (MsLS) by RNAi method. The MsLS RNAi lines exhibited a huge reduction in the synthesis of limonene and carvone. Detailed GC-MS and LC-MS analysis revealed that MsLS RNAi plants also showed an increase in sesquiterpene, phytosterols, fatty acids, flavonoids, and phenolic metabolites, suggesting an interaction between the MEP, MVA shikimate and fatty acid pathways in spearmint. Three different heterologous monoterpene synthases namely, linalool synthase and myrcene synthase from Picea abies and geraniol synthase from Cananga odorata were cloned and introduced independently into the MsLS RNAi mutant background. The expression of these heterologous terpene synthases resulted mainly in production of monoterpene derivatives. Of all the introduced monoterpenes geraniol showed the maximum number of derivatives. Our results provide new insights into MEP pathway interactions and regulation and reveals the existence of mechanisms for complex metabolism of monoterpenes in spearmint.


Assuntos
Liases Intramoleculares/genética , Mentha spicata/enzimologia , Engenharia Metabólica/métodos , Monoterpenos/metabolismo , Cananga/enzimologia , Cananga/genética , Clonagem Molecular , Regulação da Expressão Gênica de Plantas , Técnicas de Inativação de Genes , Mentha spicata/química , Redes e Vias Metabólicas , Picea/enzimologia , Picea/genética , Proteínas de Plantas/genética , Interferência de RNA
7.
J Chromatogr A ; 1618: 460853, 2020 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-31959459

RESUMO

Analyses of the complex essential oil samples using gas chromatography hyphenated with mass spectrometry (GC-MS) generate large three-way data arrays. Processing such large data sets and extracting meaningful information in the metabolic studies of natural products requires application of multivariate statistical techniques (MSTs). From the GC-MS raw data several different input data sets for the MSTs can be created, including total chromatogram average mass spectra (TCAMS), segmented average mass spectra (SAMS) and chemical composition. Herein, we compared the performance of MSTs on average mass spectrum based data sets, TCAMS and SAMS, against chemical composition and attenuated total reflectance - Fourier transformation infrared (ATR-FTIR) spectroscopy in the evaluation of quality of ylang-ylang essential oils, based on their grade, geographical origin and chemical composition, using principal component analysis (PCA), partial least squares regression (PLS) and discriminatory analysis (PLS-DA). PCA based on TCAMS, SAMS and chemical composition showed clear trends amongst the samples based on increase in grade (distillation time). PLS-DA applied to TCAMS, SAMS and ATR-FTIR discriminated between all geographical origins. Predicted relative abundances of the 18 most important compounds, using PLS regression models on TCAMS, SAMS and ATR-FTIR, were successfully applied to ylang-ylang essential oil quality assessment based on comparison with the ISO 3063:2004 standard, where the SAMS data set showed superior performance, compared to other data sets.


Assuntos
Cananga/química , Cromatografia Gasosa-Espectrometria de Massas , Óleos Voláteis/química , Óleos Vegetais/química , Destilação , Análise dos Mínimos Quadrados , Análise Multivariada , Análise de Componente Principal , Espectroscopia de Infravermelho com Transformada de Fourier
8.
Pak J Pharm Sci ; 32(1): 165-169, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30772805

RESUMO

Cananga odorata (Lamk.) Hook. f. et Thoms., belonging to Annonaceae, is an evergreen tree. The oils extracted from its flower are a famous perfume and used in daily chemical and food industry. Although this plant has been widely cultivated in tropical regions of the world, the yield of oils from its flower is very limited. In order to develop the other parts of this plant, the chemical constituents of the volatile oils from the leaves of C. odorata was analyzed by gas chromatography/flame ionization detector (GC-FID) and GC/mass spectrometry (GC-MS). And the volatiles showed nitric oxide (NO) inhibitory activity with an IC50 value of 37.61µg/mL and anti-oxidant activity with an IC50 value of 3.84mg/mL, respectively.


Assuntos
Anti-Inflamatórios/farmacologia , Antioxidantes/farmacologia , Cananga , Óleos Voláteis/farmacologia , Compostos Fitoquímicos/farmacologia , Extratos Vegetais/farmacologia , Folhas de Planta , Óleos Vegetais/farmacologia , Animais , Anti-Inflamatórios/química , Anti-Inflamatórios/isolamento & purificação , Antioxidantes/química , Antioxidantes/isolamento & purificação , Compostos de Bifenilo/química , Cananga/química , Cromatografia Gasosa-Espectrometria de Massas , Macrófagos/efeitos dos fármacos , Macrófagos/metabolismo , Camundongos , Óxido Nítrico/metabolismo , Óleos Voláteis/química , Óleos Voláteis/isolamento & purificação , Compostos Fitoquímicos/química , Compostos Fitoquímicos/isolamento & purificação , Picratos/química , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Folhas de Planta/química , Óleos Vegetais/química , Óleos Vegetais/isolamento & purificação , Células RAW 264.7
9.
Kobe J Med Sci ; 65(2): E71-E79, 2019 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-31956259

RESUMO

Chronic hepatitis B virus (HBV) infection can lead to liver cirrhosis and hepatocellular carcinoma. Current therapeutic drugs for chronic hepatitis B using pegylated interferons and nucleos(t)ide analogs have limited efficacy. Therefore, the development of novel and safe antivirals is required. Natural products including medicinal plants produce complex and structurally diverse compounds, some of which offer suitable targets for antiviral screening studies. In the present study, we screened various crude extracts from Indonesian plants for anti-HBV activity by determining their effects on the production of extracellular HBV DNA in Hep38.7-Tet cells and HBV entry onto a HBV-susceptible cell line, HepG2-NTCP, with the following results: (1) In Hep38.7-Tet cells, Cananga odorata exhibited the highest anti-HBV activity with a 50% inhibitory concentration (IC50) of 56.5 µg/ml and 50% cytotoxic concentration (CC50) of 540.2 µg/ml (Selectivity Index: 9.6). (2) The treatment of HepG2-NTCP cells with Cassia fistula, C. odorata, and Melastoma malabathricum at concentrations of 100 µg/ml lowered the levels of HBsAg production to 51.2%, 58.0%, and 40.1%, respectively, compared to untreated controls, and IC50 and CC50 values of C. odorata were 142.9 µg/ml and >400 µg/ml. In conclusion, the C. odorata extract could be a good candidate for the development of anti-HBV drugs.


Assuntos
Antivirais/análise , Cananga/química , Vírus da Hepatite B/efeitos dos fármacos , Hepatite B/tratamento farmacológico , Extratos Vegetais/uso terapêutico , Células Hep G2 , Humanos , Indonésia , Testes de Sensibilidade Microbiana , Fitoterapia , Extratos Vegetais/farmacologia , Plantas Medicinais , Replicação Viral/efeitos dos fármacos
10.
Rev. enferm. UFPE on line ; 13: [1-10], 2019. ilus, tab, graf
Artigo em Português | BDENF - Enfermagem | ID: biblio-1048160

RESUMO

Objetivo: investigar a efetividade do uso da aromaterapia com os oleos essenciais de lavanda (Lavandula angustifolia) ou ylang-ylang (Cananga odorata), associada a massagem, para o alivio da ansiedade e do estresse. Metodo: trata-se de um estudo quantitativo, exploratorio-descritivo e correlacional com delineamento quase-experimental do tipo antes e depois. Realizou-se uma intervencao com seis sessoes de massagem com aromaterapia com 21 professores de enfermagem verificado por meio de parametros psicologicos e biofisiologicos, antes e apos cada sessao da intervencao. Aplicaram-se, no inicio e ao termino da intervencao, o Inventario de Ansiedade Traco-Estado e a Lista de Sintomas de Stress. Analisaram-se os resultados com o Teste t de Student, considerando p-valor<0,05, e organizaram-se tabelas que os ilustram. Resultados: verificou-se que a pressao arterial apresentou reducao efetiva em algumas sessoes de aromaterapia. Observou-se que o estresse obteve reducao de maior magnitude quando comparado a ansiedade e o grupo que utilizou ylang-ylang, maior reducao da ansiedade que o grupo lavanda. Conclusao: demonstra-se efetividade parcial da aromaterapia associada a massagem, utilizando oleos essenciais de lavanda ou ylang-ylang em relacao aos parametros biofisiologicos.(AU)


Objective: to investigate the effectiveness of the use of aromatherapy with essential oils of lavender (Lavandula angustifolia) or ylang-ylang (Cananga odorata), associated with massage, for the relief of anxiety and stress. Method: this is a quantitative study, exploratory-descriptive and correlational with quasi-experimental design of type before and after. We performed a chart with six sessions of massage with aromatherapy with 21 teachers of nursing checked by means of psychological parameters and biophysiological, before and after each session of the intervention. There were applied, at the beginning and at the end of the intervention, the State-Trait Anxiety Inventory and the List of Symptoms of Stress. The results were analyzed with the Student t test, whereas p-value<0.05, and organized tables that illustrate them. Results: there was found that blood pressure presented effective reduction in some aromatherapy sessions. There was observed that the stress obtained reduction of greater magnitude when compared to anxiety and the group that used ylang-ylang, a greater reduction of anxiety then the group lavender. Conclusion: it is demonstrated partial effectiveness of aromatherapy associated with massage, using essential oils of lavender or ylangylang, in relation to the biophysiological parameters.(AU)


Objetivo: investigar la eficacia del uso de la aromaterapia con oleos esenciales de lavanda (Lavandula angustifolia) o ylang-ylang (Cananga odorata), asociado con el masaje, para el alivio de la ansiedad y el estres. Metodo: se trata de un estudio cuantitativo del tipo exploratorio-descriptivo y correlacional, con diseno cuasi-experimental del tipo antes y despues. Hemos realizado un grafico con seis sesiones de masaje con aromaterapia con 21 maestros de enfermeria verificados por medio de parametros psicologicos y biofisiologicos, antes y despues de cada periodo de sesiones de la intervencion. Aplica si, al comienzo y al final de la intervencion, el State-Trait Anxiety Inventory y la lista de sintomas de estres. Los resultados se analizaron con la prueba t de Student, mientras que el valor de p<0.05 y organizaron tablas que ilustrenlas. Resultados: se encontro que la presion arterial presento una reduccion eficaz en algunas sesiones de aromaterapia. Se observo que el estres obtenido reduccion de mayor magnitud en comparacion con ansiedad y el grupo que uso ylang-ylang, una mayor reduccion de la ansiedad que el grupo de lavanda. Conclusion: se demostro la eficacia parcial de la aromaterapia asociados con el masaje con aceites esenciales de lavanda o ylang-ylang, en relacion con los parametros biofisiologicos.(AU)


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Idoso , Ansiedade , Terapias Complementares , Óleos Voláteis , Saúde do Trabalhador , Aromaterapia , Lavandula , Cananga , Docentes de Enfermagem , Estresse Ocupacional , Massagem , Epidemiologia Descritiva , Educação Superior
11.
Exp Appl Acarol ; 76(2): 209-220, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30302625

RESUMO

Ylang-ylang oil (YYO) from Cananga odorata (Lam.) Hook.f. & Thomson and star anise oil (SAO) from Illicium verum Hook.f. were tested at four concentrations 0.05, 0.1, 0.2, 0.4 µl/cm2. Mortality rates were obtained by counting dead nymphs at 30-min intervals during the first 5 h after the start of exposure and then at 24, 48 and 72 h. Mortality increased with increasing oil concentration and time of exposure. The two highest concentrations of YYO (0.2, 0.4 µl/cm2) gave maximum lethal concentrations (LC) of 50 and 95% mortality after 4.5 h exposure. Mortality of 95% was obtained after 24 h with the next highest dose (0.1 µl/cm2), whereas LC95 required 3 days with the lowest YYO (0.05 µl/cm2). The lethal effect time (LT) was correlated with the duration of exposure, with a significant effect at 0.4 µl YYO/cm2 after 3 h' (LT50 = 3.2 h, LT95 = 4.3 h). In contrast, only the highest concentration of SAO, 0.4 µl SAO/cm2, showed increasing mortality with time of exposure. This reached LT50 after 10 h and LT95 after 24 h. However, with the lower concentration (0.2 µl/cm2) 50% mortality was reached after 24 h and 100% at 72 h. At to the lowest concentration of SAO (0.1 µl/cm2), 67% mortality after 48 h. The study indicates that YYO and SAO exhibit strong acaricidal properties against nymphs of I. ricinus and suggest that both YYO and SAO should be evaluated as potentially useful in the control of ticks.


Assuntos
Acaricidas , Cananga/química , Illicium/química , Ixodes , Óleos Voláteis , Controle de Ácaros e Carrapatos , Animais , Ixodes/crescimento & desenvolvimento , Ninfa/crescimento & desenvolvimento
12.
Parasitol Res ; 117(8): 2473-2485, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-29797084

RESUMO

Plasmodial resistance to artemisinin-based combination therapies emphasizes the need for new drug development to control malaria. This paper describes the antiplasmodial activity of metabolites produced by endophytic fungi of three Cameroonian plants. Ethyl acetate extracts of fungi cultivated on three different media were tested against Plasmodium falciparum chloroquine-sensitive (Pf3D7) and chloroquine-resistant (PfINDO) strains using the SYBR green florescence assay. Selected endophytes were further grown in potato dextrose broth supplemented with small organic elicitors and their extracts tested for activity. The effect of elicitors on de novo metabolite synthesis was assessed by reverse-phase HPLC. Activity screening of 81 extracts indicated that Aspergillus niger 58 (IC50 2.25-6.69 µg/mL, Pf3D7), Fusarium sp. N240 (IC50 1.62-4.38 µg/mL, Pf3D7), Phomopsis sp. N114 (IC50 0.34-7.26 µg/mL, Pf3D7), and Xylaria sp. N120 (IC50 2.69-6.77 µg/mL, Pf3D7) produced potent extracts when grown in all three media. Further culture of these endophytes in potato dextrose broth supplemented with each of the eight small organic elicitors and subsequent extracts screening indicated the extract of Phomopsis sp. N114 grown with 1% 1-butanol to be highly selective and extremely potent (IC50 0.20-0.33 µg/mL; SI > 666). RPHPLC profiles of extracts of Phomopsis sp. N114 grown with or without 1-butanol showed some peaks of enhanced intensities in the former without any qualitative change in the chromatograms. This study showed the ability of selected endophytes to produce potent and selective antiplasmodial metabolites in varied culture conditions. It also showed how the production of desired metabolites can be enhanced by use of small molecular weight elicitors.


Assuntos
Antimaláricos/farmacologia , Aspergillus niger/metabolismo , Cananga/microbiologia , Extratos Celulares/farmacologia , Fusarium/metabolismo , Plasmodium falciparum/efeitos dos fármacos , Terminalia/microbiologia , Xylariales/metabolismo , Antimaláricos/metabolismo , Artemisininas/farmacologia , Aspergillus niger/isolamento & purificação , Agentes de Controle Biológico/metabolismo , Agentes de Controle Biológico/farmacologia , Camarões , Cloroquina/farmacologia , Meios de Cultivo Condicionados , Fusarium/isolamento & purificação , Malária/tratamento farmacológico , Testes de Sensibilidade Parasitária , Extratos Vegetais/farmacologia , Plantas Medicinais/microbiologia , Xylariales/isolamento & purificação
13.
J Ethnopharmacol ; 219: 23-30, 2018 Jun 12.
Artigo em Inglês | MEDLINE | ID: mdl-29545208

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Cananga odorata essential oil, known as ylang-ylang essential oil (YYO), was commonly used in the aromatherapy for relaxation and mood adjusting use. In our previous study, YYO played anxiolytic effects on the mice in several behavioral tests that based on the instinctive responses to novel environments. AIM OF THE STUDY: To investigate the effects and mechanisms of YYO reversing the anxiety induced by 5-HT2C receptor agonist 1-(3-chlorophenyl) piperazine (m-CPP). MATERIALS AND METHODS: m-CPP was administrated to the male ICR mice to develop an anxiety model. The anxiolytic effect of YYO (0.1%, 1% and 10%, v/v) was evaluated in the elevated plus maze (EPM) test after odor exposure. Western blot was used to detect the phosphorylation levels of extracellular signal-regulated kinase 1/2 (ERK1/2) and cAMP response element-binding protein (CREB) and the expression of c-Fos in the prefrontal cortex (PFC) and hippocampus after the EPM test. Serotonin and its metabolite change in the brain were detected by liquid chromatogram with an electrochemical detector. The effect of YYO on the plasma corticosterone level was evaluated using enzyme-linked immunosorbent assay (ELISA) after the odor exposure. RESULTS: The behavior analysis showed that m-CPP (2 mg/kg and 4 mg/kg) could induce anxiety behaviors in the mice while diazepam (2 mg/kg) reversed the anxiety behavior induced by m-CPP. YYO dose-dependently increased the time and number of entries in the open arms (p < 0.05) compared to the Tween 80 group. YYO reduced the phosphorylation levels of ERK1/2 (p < 0.05) in both PFC and hippocampus. Down-regulations of phosphor-CREB (p < 0.05) and c-Fos (p < 0.05) were only observed in the hippocampus. YYO also affected the brain serotonin metabolism and reduced the blood plasma corticosterone level of the m-CPP treated mice. CONCLUSION: YYO odor exposure could reverse the anxiety behaviors generated by m-CPP. The anxiolytic effect of YYO was associated with the ERK1/2/CREB pathway in the hippocampus and relevant to the serotonin system.


Assuntos
Ansiolíticos/administração & dosagem , Ansiedade/tratamento farmacológico , Cananga , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Piperazinas/toxicidade , Agonistas do Receptor de Serotonina/toxicidade , Administração por Inalação , Animais , Ansiolíticos/isolamento & purificação , Ansiedade/induzido quimicamente , Ansiedade/metabolismo , Relação Dose-Resposta a Droga , Sistema de Sinalização das MAP Quinases/fisiologia , Masculino , Camundongos , Camundongos Endogâmicos ICR , Óleos Voláteis/isolamento & purificação , Óleos Voláteis/farmacologia , Óleos Voláteis/uso terapêutico , Óleos Vegetais/administração & dosagem , Óleos Vegetais/isolamento & purificação , Serotonina/metabolismo
14.
Chem Pharm Bull (Tokyo) ; 65(1): 97-101, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28049920

RESUMO

From the leaves of Cananga odorata var. odorata, three relatively large molecules, namely two aryl naphthalene lignan diesters of canangafruticoside A and one cyclobutane lignan diester of canangafruticoside A, were isolated along with four known compounds. The structures of the new compounds were elucidated based on spectroscopic evidence.


Assuntos
Cananga/química , Ésteres/isolamento & purificação , Lignanas/isolamento & purificação , Folhas de Planta/química , Ésteres/química , Lignanas/química , Conformação Molecular
15.
Dermatitis ; 28(1): 14-21, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28002230

RESUMO

In this article, some aspects of sandalwood oil, ylang-ylang oil, and jasmine absolute are discussed including their botanical origin, uses of the plants and the oils and absolute, chemical composition, contact allergy to and allergic contact dermatitis from these essential oils and absolute, and their causative allergenic ingredients.


Assuntos
Cananga/química , Dermatite Alérgica de Contato/etiologia , Jasminum/química , Óleos Voláteis/farmacologia , Óleos Vegetais/farmacologia , Pele/efeitos dos fármacos , Aromaterapia , Humanos , Óleos Voláteis/efeitos adversos , Testes do Emplastro , Óleos Vegetais/efeitos adversos , Sesquiterpenos/efeitos adversos , Sesquiterpenos/farmacologia
16.
Phytomedicine ; 23(14): 1727-1734, 2016 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-27912874

RESUMO

BACKGROUND: Essential oil from Cananga odorata (ylang-ylang essential oil, YYO) is usually used in reducing blood pressure, improving cognitive functioning in aromatherapy in human. Few reports showed its effect on anxiety behaviors. HYPOTHESIS/PURPOSE: To investigate the anxiolytic effects of YYO exposure on anxiety animal models, determine the major active constituents and investigate the change of neurotransmitters after odor exposure. STUDY DESIGN AND METHODS: ICR mice were subjected to three anxiety models including open field, elevated plus maze and light-dark box tests after acute and chronic YYO exposure. Main constituents of YYO were defined using GC/MS. These compounds were then tested on the male mice separately on three anxiety models. The monoamines neurotransmitters and their metabolites were analyzed after acute odor exposure and elevated plus maze test. RESULTS: YYO exposure only showed significant anxiolytic effect on the male mice. It increased the time that mice visited open arms and light box area in elevated plus maze and light-dark box tests after acute and chronic YYO exposures. Three main constituents of YYO, benzyl benzoate, linalool and benzyl alcohol showed anxiolytic effect on the male mice individually. YYO exposure brought changes of neurotransmitters on the male mice more significantly than the female mice. It decreased the dopamine (DA) concentration in the striatum and increased the 5-hydroxytryptamine (5-HT) concentration in the hippocampus of the male mice. The major constituent benzyl benzoate changed neurotransmitters concentration in accordance with the YYO. Moreover, it decreased the ratio of 5-HIAA/5-HT in the hippocampus. CONCLUSION: Both acute and chronic YYO exposure showed anxiolytic effect on the male mice. YYO and its major constituent benzyl benzoate might act on the 5-HTnergic and DAnergic pathways.


Assuntos
Ansiolíticos/farmacologia , Ansiedade/metabolismo , Cananga/química , Dopamina/metabolismo , Óleos Voláteis/farmacologia , Extratos Vegetais/farmacologia , Serotonina/metabolismo , Monoterpenos Acíclicos , Animais , Ansiolíticos/uso terapêutico , Ansiedade/tratamento farmacológico , Aromaterapia , Comportamento Animal/efeitos dos fármacos , Benzoatos/farmacologia , Benzoatos/uso terapêutico , Álcool Benzílico/farmacologia , Álcool Benzílico/uso terapêutico , Encéfalo/efeitos dos fármacos , Encéfalo/metabolismo , Feminino , Humanos , Ácido Hidroxi-Indolacético/metabolismo , Masculino , Camundongos Endogâmicos ICR , Monoterpenos/farmacologia , Monoterpenos/uso terapêutico , Óleos Voláteis/química , Óleos Voláteis/uso terapêutico , Fitoterapia , Extratos Vegetais/química , Extratos Vegetais/uso terapêutico , Óleos Vegetais/química , Óleos Vegetais/farmacologia , Óleos Vegetais/uso terapêutico
17.
Fitoterapia ; 114: 45-50, 2016 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-27575324

RESUMO

Chemical investigation of the roots of Cananga latifolia led to the isolation and purification of thirteen juvenile hormone III analogues. Six new analogues, canangalias C-H (1-6) and a new natural product, (2E,6E,10R)-10-acetoxy-11-hydroxy-3,7,11-trimethyldodeca-2,6-dienoic acid methyl ester (7), were isolated. In addition, six known juvenile hormone III analogues were isolated. Their structures were established by spectroscopic methods including 1D and 2D NMR, IR and mass spectrometry.


Assuntos
Cananga/química , Compostos Fitoquímicos/química , Raízes de Plantas/química , Sesquiterpenos/química , Antifúngicos/química , Antifúngicos/isolamento & purificação , Espectroscopia de Ressonância Magnética , Espectrometria de Massas , Estrutura Molecular , Compostos Fitoquímicos/isolamento & purificação , Pythium/efeitos dos fármacos , Sesquiterpenos/isolamento & purificação
18.
Dermatitis ; 27(4): 170-5, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27427818

RESUMO

Nearly 80 essential oils (including 2 jasmine absolutes) have caused contact allergy. Fifty-five of these have been tested in consecutive patients suspected of contact dermatitis, and nine (laurel, turpentine, orange, tea tree, citronella, ylang-ylang, sandalwood, clove, and costus root) showed greater than 2% positive patch test reactions. Relevance data are generally missing or inadequate. Most reactions are caused by application of pure oils or high-concentration products. The clinical picture depends on the responsible product. Occupational contact dermatitis may occur in professionals performing massages. The (possible) allergens in essential oils are discussed. Several test allergens are available, but patients should preferably be tested with their own products. Co-reactivity with other essential oils and the fragrance mix is frequent, which may partly be explained by common ingredients. Patch test concentrations for essential oils are suggested.


Assuntos
Dermatite Alérgica de Contato/etiologia , Óleos Voláteis/efeitos adversos , Cananga/efeitos adversos , Óleo de Cravo/efeitos adversos , Dermatite Alérgica de Contato/epidemiologia , Humanos , Óleos Vegetais/efeitos adversos , Sesquiterpenos/efeitos adversos , Óleo de Melaleuca/efeitos adversos , Terebintina/efeitos adversos
19.
Pediatr Emerg Care ; 32(9): 642-4, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-26890296

RESUMO

A well-appearing 3-year-old boy presented to the pediatric emergency department 2 hours after a presumed hydrocarbon ingestion. He was referred to the emergency department by his pediatrician after consultation with the local poison control center after possibly ingesting ylang ylang (Cananga odorata) fragrance oil. The child was asymptomatic with a normal physical examination. Point-of-care lung ultrasound identified focal hydrocarbon pneumonitis in the right lung and demonstrated resolution of these findings. Utilization of point-of-care ultrasound resulted in a shorter emergency department length of stay and the avoidance of radiation exposure from serial chest x-rays.


Assuntos
Cananga/envenenamento , Hidrocarbonetos/envenenamento , Pulmão/diagnóstico por imagem , Óleos Vegetais/envenenamento , Pneumonia/diagnóstico por imagem , Sistemas Automatizados de Assistência Junto ao Leito , Ultrassonografia/métodos , Pré-Escolar , Serviço Hospitalar de Emergência , Humanos , Pulmão/efeitos dos fármacos , Masculino , Pediatria , Pneumonia/induzido quimicamente
20.
Contact Dermatitis ; 74(4): 236-41, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26806578

RESUMO

BACKGROUND: Essential oils are fragrance substances that are labelled on cosmetic products by their INCI names, potentially confusing consumers. OBJECTIVES: To establish whether contact allergy to essential oils might be missed if not specifically tested for. METHODS: We tested 471 patients with 14 essential oils and 2104 patients with Melaleuca alternifolia oil between January 2008 and June 2014. All patients were tested with fragrance mix I, fragrance mix II, hydroxyisohexyl 3-cyclohexene carboxaldehyde, and Myroxylon pereirae. Three hundred and twenty-six patients were tested with hydroperoxides of limonene and linalool. RESULTS: Thirty-four patients had a +/++/+++ reaction to at least one essential oil. Eleven had no reaction to any of the six marker fragrance substances. Thus, 4 of 11 positive reactions to M. alternifolia oil, 2 of 7 reactions to Cymbopogon flexuosus oil, 1 of 5 reactions to Cananga odorata oil, 3 of 4 reactions to Santalum album oil and 2 of 3 reactions to Mentha piperita oil would have been missed without individual testing. CONCLUSION: A small number of patients who are allergic to essential oils could be missed if these are not specifically tested. Labelling by INCI names means that exposure may not be obvious. Careful inspection of so-called 'natural' products and targeted testing is recommended.


Assuntos
Alérgenos/efeitos adversos , Dermatite Alérgica de Contato/etiologia , Óleos Voláteis/efeitos adversos , Perfumes/efeitos adversos , Monoterpenos Acíclicos , Aldeídos/efeitos adversos , Cananga/efeitos adversos , Cosméticos/efeitos adversos , Cosméticos/química , Cicloexenos/efeitos adversos , Cymbopogon/efeitos adversos , Humanos , Limoneno , Monoterpenos/efeitos adversos , Myroxylon/efeitos adversos , Testes do Emplastro , Óleos Vegetais/efeitos adversos , Rotulagem de Produtos , Estudos Retrospectivos , Santalum/efeitos adversos , Óleo de Melaleuca/efeitos adversos , Terpenos/efeitos adversos
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