Evaluation of cellophane as platform for colorimetric assays on microfluidic analytical devices.

Due to their low cost, simplicity, and pump-free liquid transport properties, colorimetric assays on paper spots and microfluidic paper-based analytical devices (µPADs) are regarded as useful tools for point-of-care testing (POCT). However, for certain types of colorimetric assays, the "non-transparent" and "white" characters of paper can be a disadvantage. In this work, the possibilities of using cellophane as an alternative platform for colorimetric assays have been investigated. Cellophane is a low cost and easy-to-handle transparent film made of regenerated cellulose. Owing to its hydrophilic character, cellophane-based microfluidic channels fabricated through a print-cut-laminate approach enabled pump-free liquid transport into multiple detection areas, similar to µPADs. In addition, the water absorption characteristics of cellophane allowed the stable immobilization of water-soluble colorimetric indicators without any surface modification or additional reagents. The transparency of cellophane provides possibilities for simple background coloring of the substrates, increasing the dynamic signal range for hue-based colorimetric assays, as demonstrated for two model assays targeting H2O2 (46-fold increase) and creatinine (3.6-fold increase). Finally, a turbidity detection-based protein assay was realized on black background cellophane spots. The lowest limits of detection achieved with the cellophane-based devices were calculated as 7 µM for H2O2, 2.7 mg dL-1 for creatinine, and 3.5 mg dL-1 for protein (human serum albumin).

Human exposure to microplastics from urban decentralized pay-to-fetch drinking-water refill kiosks.

Microplastics in the human diet have become a worldwide concern. To date, microplastics in urban drinking water supplies, such as decentralized drinking-water refill kiosks, have not been studied and are a pressing concern since they are so closely tied to human life and have a significant influence on health. This study evaluated the occurrence and characteristics of microplastics in 63 drinking water samples collected from decentralized refill kiosks in the Mexico City metropolitan area. All of the sampled drinking water contained microplastics in concentrations ranging from 11 to 860 microplastics L-1. The detected microplastics were mostly fiber (65 %), followed by fragment (28 %), and film (7 %). They were mainly composed of polyethylene terephthalate, polyamides, vinyl polymers, polyacetal, and cellophane in sizes ranging from 20 µm to 5 mm, with 75 % of them accounting for sizes <300 µm. SEM-EDX analysis revealed weathered microplastics, biota adherence, and the presence of inorganic elements on the surface of microplastics. We estimate that Mexico City residents inadvertently ingest 42 microplastics L-1, with an annual exposure of around 1.47 × 104 microplastics per adult and 6.73 × 103 microplastics per child. Therefore, future research is needed to strengthen drinking water refill kiosk guidelines and standards for better microplastic management. This study serves as a wake-up call to many developing countries that use similar urban water systems, drawing their attention to global microplastic contamination of drinking water.

Occurrence and characteristics of atmospheric microplastics in Mexico City.

While atmospheric microplastics have attracted scientific attention as a significant source of microplastic contamination in the environment, studies in large population centers remain sparse. Here we present the first report on the occurrence and distribution of atmospheric microplastics in Mexico City (Latin America's second most densely populated city), collected using PM10 and PM2.5 active samplers at seven monitoring stations (urban, residential, and industrial) during the dry and wet seasons of 2020. The results showed that microplastics were detected in all of the samples examined, with mean microplastic concentrations (items m-3) of 0.205 ± 0.061 and 0.110 ± 0.055 in PM10 and PM2.5, respectively. The spatial distribution of microplastics showed seasonal variation, with greater abundances in locations closer to industrial and urban centers. There was also a significant difference in microplastic concentrations in PM10 and PM2.5 between the dry and wet seasons. The mean PM2.5/PM10 ratio was 0.576, implying that microplastics were partitioned more towards PM2.5 than PM10 in Mexico City. Fibers were the most prominent shape (>75 %), and blue was the most common color (>60 %). The size characteristics indicated microplastics of varying lengths, ranging from 39 to 5000 µm, with 66 % being <500 µm. Metal contaminants such as aluminum, iron, and titanium were detected using SEM-EDX on randomly selected microplastics. The microplastics were identified as cellophane, polyethylene, polyethylene terephthalate, polyamide, and cellulose (rayon) using ATR-FTIR spectral analysis. Our findings unravel the extent and characteristics of atmospheric microplastics in the Mexico City metropolitan area, which will aid future research to better understand their fate, transport, and potential health risks, demanding more investigations and close monitoring.

Identification and Functional Analysis of a Novel Hydrophobic Protein VdHP1 from Verticillium dahliae.

Verticillium dahliae could cause destructive vascular wilt disease on hundreds of plant species around the world, including cotton. In this study, we characterized the function of a hydrophobin gene VdHP1 in pathogen development and pathogenicity. Results showed that VdHP1 could induce cell death and activate plant immune responses. The VdHP1 deletion mutants (ΔVdHP1) and the complement mutants (C-ΔVdHP1) were obtained by the homologous recombination method. The VdHP1 deletion mutants exhibited increased hydrophilicity, inhibited microsclerotial formation, and reduced spore smoothness. In addition, the deletion mutants were more sensitive to NaCl, while relatively insensitive to KCl and sorbitol. Mutants also had greater resistance to Congo red, UV radiation, and high temperature, which suggested that ΔVdHP1 strains have stronger resistance to abiotic stress in general. Different carbon source assays showed that the utilization ability of skim milk, cellulose, and starch was greatly enhanced in ΔVdHP1, compared with that of WT and complemented strains. Furthermore, VdHP1 did not affect mycelium penetration on cellophane but contributed to mycelium growth on surface of the living plant cells. The pathogenicity test found that the crude toxin content, colonization, and dispersal of ΔVdHP1 was significantly increased compared with the WT and complementary strains. In addition, cotton seedlings showed more severe wilting symptoms after inoculation with ΔVdHP1 strains. These results suggested that the hydrophobin VdHP1 negatively regulated the virulence of V. dahliae, and played an important role in development, adaptability, and pathogenicity in V. dahliae, which maybe provide a new viewpoint to further understand the molecular mechanisms of pathogen virulence. IMPORTANCE Verticillium dahliae is a soilborne fungal pathogen that causes a destructive vascular disease on a large number of plant hosts, resulting in great threat to agricultural production. In this study, it was illustrated that the hydrophobin VdHP1 could induce cell death and activate plant immune responses. VdHP1 affected the hydrophobicity of V. dahliae, and negatively regulated the strains resistant to stress, and the utilization ability of different carbon sources. In addition, VdHP1 did not affect mycelium penetration on cellophane but contributed to mycelium growth on surface of the living plant cells. The VdHP1 gene negatively regulated the total virulence, colonization, and dispersal of V. dahliae, with enhanced pathogenicity of mutant strains in this gene. These results suggested that the hydrophobin VdHP1 played an importance in development, adaptability, and pathogenicity in V. dahliae, and would provide a new viewpoint to further understand the molecular mechanisms of pathogen virulence.

Packaging Characteristics of Top-Selling Cigars in the United States, 2018.

INTRODUCTION: Tobacco product packaging is an influential industry marketing tool, but research on cigar packaging characteristics is limited. This study leveraged a pack archive of the top-selling, mass-market cigar products in the US and their corresponding sales data to assess the prevalence of common pack features in the marketplace. METHODS: The 50 cigar products with the highest national unit sales in 2018 were identified using Nielsen's Scantrack data. A content analysis captured common packaging features within four domains: physical, textual, graphical, and promotional elements. Descriptive statistics, weighted by each pack's 2018 unit sales, documented the market share of pack characteristics overall and by brand. RESULTS: Products in the sample constituted 62% of all convenience store cigar sales in 2018. Black & Mild, the most popular brand, did not display warning labels on its single stick, cellophane-wrapped products, which constituted nearly all of its sales (96.3%). Resealable foil pouches were the most common packaging style across other brands, and were most often sold in predominantly red, green, or silver packaging. Common flavor categories included concept (eg, "Jazz", 32.4%), fruit (15.7%), and sweet (14.5%). Prepricing (eg, "2 for 99 cents") was a popular promotional strategy, appearing on 74% of all packs sold. CONCLUSIONS: Cigar packaging features such as small pack sizes, innovative materials, flavor names, bright colors, and cost-saving promotions are prevalent among the top-selling products. Stronger packaging-focused policies at the federal and local levels may help reduce appeal and potentially curb cigar use. IMPLICATIONS: Sales of mass-market cigars in the US have remained strong over the past decade, propelled by lax product regulations. This study demonstrated that cigar packaging features that may enhance consumer appeal, such as small pack sizes, flavor names, sensory descriptors, bright colors, and cost-saving promotions, are prevalent among the top-selling products. Stronger packaging-focused policies at the federal and local levels may help curb cigar use, reduce youth appeal, and potentially minimize existing disparities in cigar use.

The cellophane bees of Colletes Latreille (Hymenoptera: Colletidae) from Xizang (Tibet), China.

We present information about the known species of the genus Colletes Latreille found in Xizang (China), including an integrative approach to taxonomy for many of the species. A new species belonging to the C. clypearis Morawitz group, C. splendidus Ferrari, Niu Zhu sp. nov., is described. Colletes sichuanensis Kuhlmann and C. tuberculatus Morawitz are recorded from Xizang for the first time. The suspected occurrences of both C. bischoffi Noskiewicz and C. packeri Kuhlmann in China are confirmed and their presence in Xizang are also established for the first time. We show that C. fulvicornis Noskiewicz was wrongly recorded from Xizang previously and, therefore, the species is herein removed from the list of the Tibetan Colletes. Thus, 26 species of the genus are now known from Xizang. The putative occurrence of C. annejohnae Kuhlmann in Xizang could not be confirmed, but the species is nonetheless included in the scope of this paper to facilitate its identification by future studies. An illustrated key for the Tibetan species of Colletes is provided.

CgEnd3 Regulates Endocytosis, Appressorium Formation, and Virulence in the Poplar Anthracnose Fungus Colletotrichum gloeosporioides.

The hemibiotrophic ascomycete fungus Colletotrichum gloeosporioides is the causal agent of anthracnose on numerous plants, and it causes considerable economic losses worldwide. Endocytosis is an essential cellular process in eukaryotic cells, but its roles in C. gloeosporioides remain unknown. In our study, we identified an endocytosis-related protein, CgEnd3, and knocked it out via polyethylene glycol (PEG)-mediated protoplast transformation. The lack of CgEnd3 resulted in severe defects in endocytosis. C. gloeosporioides infects its host through a specialized structure called appressorium, and ΔCgEnd3 showed deficient appressorium formation, melanization, turgor pressure accumulation, penetration ability of appressorium, cellophane membrane penetration, and pathogenicity. CgEnd3 also affected oxidant adaptation and the expression of core effectors during the early stage of infection. CgEnd3 contains one EF hand domain and four calcium ion-binding sites, and it is involved in calcium signaling. A lack of CgEnd3 changed the responses to cell-wall integrity agents and fungicide fludioxonil. However, CgEnd3 regulated appressorium formation and endocytosis in a calcium signaling-independent manner. Taken together, these results demonstrate that CgEnd3 plays pleiotropic roles in endocytosis, calcium signaling, cell-wall integrity, appressorium formation, penetration, and pathogenicity in C. gloeosporioides, and it suggests that CgEnd3 or endocytosis-related genes function as promising antifungal targets.

Changes to the littered cigarette pack environment in New York City between 2011 and 2015.

INTRODUCTION: Imposing policies that increase tobacco prices is a key strategy for reducing smoking prevalence, although it may result in more cigarette trafficking. In 2013, New York City (NYC) passed the Sensible Tobacco Enforcement (STE) law requiring cigarettes be sold for a minimum price of $10.50 per pack. To evaluate whether cigarette price increases changed patterns of behaviour related to cigarette tax evasion, we examined littered pack study data from 2011 and 2015. METHODS: Littered cigarette packs were collected from a random sample of NYC census tracts in 2011 and 2015. The proportions of cigarette packs with proper local, known non-local, foreign or unknown, and no tax stamp were calculated. Changes in volume, source and consumption of domestically trafficked cigarettes over time were estimated. RESULTS: In 2011, 255 packs with cellophane were collected; in 2015, 226 packs with cellophane were collected. Packs without proper local stamp increased from 60.7% in 2011 to 76.3% in 2015 (p<0.05) and those with foreign or unknown stamp increased from 11.6% in 2011 to 31.4% in 2015 (p<0.05). The percentage of domestically sourced packs attributed to domestic trafficking increased significantly from a range of 47.9% to 52.8% in 2011 to a range of 59.4% to 63.2% in 2015. CONCLUSION: While the trafficking rate among domestically sourced cigarettes increased between 2011 and 2015 (before and after the STE minimum price floor on cigarette packs was in place), there was a decline in total consumption of domestically trafficked cigarettes due to a significant increase in consumption of foreign-sourced cigarettes. Jurisdictions considering price measures should bolster monitoring and enforcement efforts to maximise public health impact. Given the interstate nature of cigarette trafficking in the USA, Federal intervention would be optimal.

Complications and outcome of cats with congenital extrahepatic portosystemic shunts treated with thin film: Thirty-four cases (2008-2017).

BACKGROUND: Congenital extrahepatic portosystemic shunts (CEHPSS) are rare in cats. Outcome after attenuation of CEHPSS with thin film has been described in a small number of cases. OBJECTIVES: To describe the clinical presentation, postoperative complications, and outcome of cats treated with thin film to attenuate CEHPSS. ANIMALS: Thirty-four cats with CEHPSS were identified from the database of 3 institutions over 9 years. METHODS: Retrospective study. Medical records were reviewed to identify cats with a diagnosis of a CEHPSS that underwent surgical attenuation. Congenital extrahepatic portosystemic shunts were suspected from clinical signs, clinicopathologic findings, and diagnostic imaging, and confirmed at exploratory laparotomy. Cats treated with thin film band attenuation were included. Postoperative complications and follow-up were recorded. RESULTS: Complications were recorded in 11 of 34 cats. Deaths related to CEHPSS occurred in 6 of 34; 4 cats did not survive to discharge. Persistent seizures were the cause of death in 4 cats. Seizures were recorded in 8 of 34 cats after surgery; all these cats received preoperative antiepileptic drugs. Serum bile acid concentrations normalized in 25 of 28 of the cats for which data was available. Three cats had persistently increased serum bile acid concentrations and underwent a second exploratory laparotomy. One had a patent shunt, the other 2 had multiple acquired portosystemic shunts. Median follow-up was 8 months (0.5-84 months). CONCLUSIONS AND CLINICAL IMPORTANCE: Congenital extrahepatic portosystemic shunts attenuation using thin film in cats carries a good short- and mid-term prognosis if they survive the postoperative period. Seizures were the most common cause of death.

Microplastic abundance, distribution and composition in the Pearl River along Guangzhou city and Pearl River estuary, China.

Like many urban rivers, the Pearl River in China is contaminated with microplastics. Compared with marine environments, microplastic pollution in freshwater is less understood, especially in urban rivers. In the present study, the abundance and distribution of microplastics in water from the Pearl River was investigated, including the estuary and the urban section along Guangzhou. The average abundance of microplastics was 19,860 items/m3 and 8902 items/m3 in the urban section and estuary, respectively. Wastewater effluents from cities might be a main source of microplastics in the Pearl River, and the urban tributaries might act as retention systems for microplastics. Among these microplastics, over 80% of them were less than 0.5 mm. The main shapes of microplastics were film, fragment, and fiber, mostly blue or transparent. Moreover, the most common polymer types of these microplastics were polyamide (26.2%) and cellophane (23.1%). This study reveals the contamination and characteristics of microplastics in the Pearl River, and provides important data for further research on microplastics in freshwater ecosystems.

Evaluation of different methods of securing cellophane bands for portosystemic shunt attenuation.

OBJECTIVE: To compare mechanisms of and pressures at failure of 4 methods of securing 2 types of cellophane bands around a vein. STUDY DESIGN: Ex vivo mechanical evaluation. METHODS: Cellophane bands composed of 3 or 4 layers were applied around a cadaveric external jugular vein (EJV) to create 25% or 50% attenuation. These bands were secured with a medium or medium-large polymer locking ligation clip (PLLC), or a medium or medium-large titanium ligation clip (TLC). Sterile saline 0.9% was instilled into the lumen of the EJV until a pressure of 100 mm Hg was reached. Failure mechanism and luminal pressure at failure were compared between groups. RESULTS: Medium clips failed less often than medium-large clips (P < .001) and consistently sustained 100 mm Hg without failing. Three-layer cellophane bands were 4.1 times more likely to fail than 4-layer bands (P = .003, CI 1.6-10.2) and failed at lower pressures (28.32 ± 3.59 mm Hg and 44.81 ± 6.51 mm Hg, respectively, P = .027). Failure rates of the cellophane band constructs did not differ whether secured with PLLC or with TLC (P = .635) or with 25% vs 50% attenuation (P = .780). CONCLUSION: A single medium clip withstood physiological forces and secured a cellophane band at up to 50% attenuation. A 3-layer cellophane band was more likely to fail compared with a 4-layer cellophane band. CLINICAL SIGNIFICANCE: These ex vivo results provide evidence to support the application of a 4-layer cellophane band secured with a single medium PLLC or TLC for portosystemic shunt attenuation. A single medium PLLC should be used to eliminate computed tomography artifacts during postoperative evaluation of shunt closure.

Potassium hydroxide mount with cellophane adhesive tape: a method for direct diagnosis of dermatophyte skin infections.

BACKGROUND: Potassium hydroxide (KOH) mount is a simple and rapid procedure that can be performed with minimal resources and provides results within 1-2 h. AIM: To further optimize KOH mount using transparent cellophane tape to ascertain fungal elements, even by inexperienced personnel. METHODS: A split-body study was conducted on 100 patients with tinea cruris. Conventional scrape method was performed on the right side and the cellophane tape method on the left. Microscopy was performed 1-2 h after collection, and on the next morning and days 3 and 7. RESULTS: Of the 100 samples, positive results were seen in 91 (91%) with the standard method and 94 (94%) with the cellophane tape method. Clumping of the fungi and presence of artefacts were more marked with the scrape than with the tape method. CONCLUSION: In view of its merits over the conventional scrape method, the cellophane tape method is a prudent alternative for the detection of superficial fungal infections.

Outcomes of cellophane banding or percutaneous transvenous coil embolization of canine intrahepatic portosystemic shunts.

OBJECTIVE: To compare clinical outcomes of dogs with congenital intrahepatic portosystemic shunts (CIHPSS) treated with cellophane banding (CB) or percutaneous transvenous coil embolization (PTCE). STUDY DESIGN: Dual-institutional retrospective study. ANIMALS: Fifty-eight dogs with CIHPSS (2001-2016). METHODS: Medical records of dogs undergoing CB or PTCE for CIHPSS were reviewed for signalment, body weight, hematologic values, shunt location, attenuation technique, procedure time, duration of hospitalization, complications, date of follow-up, and cause of death if applicable. RESULTS: Thirty-one dogs underwent CB, and 27 dogs underwent PTCE. No differences were detected between groups for gender, preoperative packed cell volume, albumin, cholesterol, or bile acids. Body weight was greater in dogs treated via PTCE. Shunts differed in location because dogs undergoing CB were diagnosed with more left divisional shunts compared with PTCE dogs. Procedural duration of CB and PTCE did not differ. Dogs treated with CB sustained more minor postoperative complications and were hospitalized longer than dogs treated with PTCE. The 1-year and 2-year survival rates were 89% for the CB group and 87% and 80% for the PTCE group, respectively. The proportion surviving at 5 years was 75% and 80% for CB dogs and PTCE dogs, respectively. CONCLUSION: CB and PTCE are associated with similar short-term and intermediate-term survival. PTCE is a minimally invasive alternative to CB via celiotomy. However, CB allows concurrent abdominal procedures requiring the same approach.

Comparative outcomes between ameroid ring constrictor and cellophane banding for treatment of single congenital extrahepatic portosystemic shunts in 49 dogs (1998-2012).

OBJECTIVE: To compare outcomes and identify prognostic factors in dogs with single congenital extrahepatic portosystemic shunt (CEHPSS) gradually attenuated with an ameroid ring constrictor (ARC) or cellophane banding (CB). STUDY DESIGN: Retrospective, multi-institutional study. ANIMALS: Forty-nine dogs with CEHPSS (n = 23 for ARC; n = 26 for CB). METHODS: Medical records of dogs with CEHPSS treated by ARC or CB were reviewed for postoperative (<1 month), midterm (1-6 months), and long-term (> 6 months) outcomes. Data were evaluated to detect factors associated with postoperative complications, residual shunting, and long-term outcome. RESULTS: Postoperative complication rates did not differ between ARC (26.1%) and CB (23.1%, P = .89) and were negatively associated with body weight (P = .03). Overall, postoperative mortality was low (2.0%). Clinical long-term outcome was excellent in 45.0% and 39.1% and good in 55.0% and 60.9% of dogs after ARC and CB, respectively. Suspected residual shunting rate upon abdominal ultrasonography was greater after CB (31.6%) than after ARC (0%). CONCLUSION: ARC and CB were both effective for attenuation of CEHPSS, resulting in good to excellent outcomes with low morbidity and mortality. Residual shunting was suspected in a higher proportion of dogs treated with CB on the basis of abdominal ultrasonography results. However, further prospective randomized studies must be conducted with validated evaluation methods to verify this assumption.

Annual Report on the External Quality Assessment Scheme for Clinical Parasitology in Korea (2017)

Annual proficiency surveys were conducted in March, May, and August of 2017 as the Korean Association of External Quality Assessment Service. Overall, four image samples (MPI-17-01, MPI-17-02, MPI-17-03, MPI-17-04) in the first trial, three image samples (MPI-17-05, MPI-17-06 , MPI-17-07) in the second trial, and a slide specimen (MPS-17-01) using parasite samples in the third trial were distributed to participating institutions. The first and second trial specimens were prepared by photographing slides made of formalin-ether concentrate of positive samples stored for educational purposes. The slide distributed in the third trial was prepared using cellophane tape, which was stored after diagnosis of the patients infected with Enterobius vermicularis . There were 191 participating institutions in the first, 204 in the second, and 212 in the third trial. The correct identification rates were 27.2% for MPI-17-01 Diphyllobothrium species (sp.), 96.6% for MPI-17-02 no parasite, 67.5% for MPI-17-03 Metagonimus yokogawai , 71.2% for MPI-17-04 Balantidium coli , 99.0% for MPI-17-05 Taenia sp., 99.0% for MPI-17-06 Trichuris trichiura , 92.7% for MPI-17-07 Cryptosporidium sp., and 96.7% for MPS-17-01 E. vermicularis . The current external quality assessment for clinical parasitology was performed using image samples and standard slides. Surveys of parasitic infections should be accompanied by continuous education on various parasitic infections, for which there was lack of experience of inspection in clinical laboratories. In the future, it will be necessary to establish a standard material using parasitic samples, and ultimately to conduct a survey on whole series of tests for the diagnosis of parasitic diseases.

Cling film as storage medium for avulsed teeth. An in vitro pilot study

The long-term prognosis of avulsed teeth primarily depends on the behavior at the scene of the accident. Lay people are not able to perform an immediate replantation. Therefore, particular significance belongs to the cellphysiologic storage of avulsed teeth. The aim of this pilot study was to evaluate whether cling film facilitates the survival of periodontal ligament cells in vitro. For this purpose, healthy human third molars were used. They were cut into root slices, which were stored in one of five test media: SOS Zahnbox ® , UHT milk (4 °C), sterile isotonic saline solution, tap water, and cling film. Following storage periods of 2 hours, 6 hours, and 24 hours in the respective medium, slices were cultivated at 37 °C and 5% CO 2 . After 2 days, 7 days, and 14 days in culture, surviving periodontal ligament cells of each slice were assessed quantitatively. Apart from tap water, all investigated media promoted cell survival. At the time of 2 hours, storage in cling film facilitated the highest cell growth compared to all other media. At the time of 6 hours, teeth stored in cling film sho wed cell growth comparable to that observed in the SOS Zahnbox ®. The results of this pilot study indicate that cling film possibly could be used as an alternative transport medium for a storage period of up to 6 hours.

Computed tomographic imaging and mechanical analysis of cellophane banding secured with locking polymer clips for portosystemic shunts in canine cadavers.

OBJECTIVE To determine whether cellophane banding secured with locking polymer clips on cadaveric splenic veins would cause less CT imaging artifact and achieve equivalent mechanical strength, compared with cellophane banding secured with metal vascular clips. ANIMALS 10 canine cadavers. PROCEDURES Clips of each material were applied to each cadaver in a crossover design study. Triple-layer cellophane bands secured with 4 medium-large or large polymer or metal clips were placed on cadaveric splenic veins and evaluated by use of CT. Beam-hardening artifact was assessed by artifact length, attenuation, and a subjective grading scale ranging from 1 to 3 for mild to severe imaging artifacts. Secured cellophane bands were mechanically tested to determine force-deformation curves and yield forces. Findings for clip methods were compared with a 1-way ANOVA with a Tukey post-test. RESULTS For metal clips, beam-hardening artifact lengths and subjective artifact grades were significantly higher, whereas attenuation values were significantly lower, than findings for polymer clips. Polymer clips were significantly lower in strength than metal clips with mean ± SD yield loads of 1.9 ± 0.6 N (medium-large polymer clips), 2.8 ± 1.3 N (large polymer clips), 6.0 ± 1.9 N (medium-large metal clips), and 8.4 ± 2.7 N (large metal clips). CONCLUSIONS AND CLINICAL RELEVANCE Use of locking polymer clips to secure cellophane banding resulted in less CT imaging artifact and mechanical strength, compared with use of metal vascular clips. Use of locking polymer clips may allow improved assessment of postoperative CT imaging in dogs with extrahepatic portosystemic shunts, which warrants in vivo clinical evaluation.

Simple Inkjet Process To Fabricate Microstructures of Chitinous Nanocrystals for Cell Patterning.

Structural polysaccharide nanocrystals (NCs) including cellulose nanocrystal have attracted attention. In order to broaden the range of application of the NCs, we can take advantage of their original characteristics by establishing simple and reasonable processing methods. We here demonstrate a micropatterning of animal cellular adhesion by inkjet printing of aqueous dispersions of cytocompatible chitinous NCs onto cellophane films. We display how to regulate the deposition form and two-dimensional shape of the chitinous NC micromoldings using a research inkjet printer. Adhesive capability of mouse fibroblasts onto the chitinous substrates was greatly improved by alkali deacetylation. The deacetylated products remained rod-like nanostructures, but the original chitin crystal form changed to that of chitosan by an intensive deacetylation. The adhered cells could be recovered glycolytically. The chitinous micropatterning substrates can be utilized for biomedical applications such as controlling of cellular shapes, precise monitoring molecular events in biochemistry, and drug screening.