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1.
Jpn J Infect Dis ; 75(2): 148-155, 2022 Mar 24.
Artigo em Inglês | MEDLINE | ID: mdl-34470967

RESUMO

Citrobacter koseri (C. koseri) is an opportunistic pathogen that can cause a variety of diseases. Although the mortality rate of C. koseri infections is high, there is a paucity of clinical information. Furthermore, the genomic features of this species are poorly understood. Herein, we present a patient with endogenous endophthalmitis secondary to septicemia, and collected a C. koseri isolate, CKNJ, from the blood of the patient. Whole genome sequencing revealed that CKNJ harbors no plasmids and codes for 67 putative virulence factors. Whole genome single nucleotide polymorphism-based phylogenetic analysis revealed that the CKNJ strain was close to strains with the same isolation sites. Compared to the other sequenced C. koseri chromosomes, CKNJ contains several strain-variable regions, including one prophage and 2 large genomic islands. Sequencing of the first complete genome of a clinical strain from China should reinforce our understanding of the genomic features and pathogenicity of this invasive infection-causing C. koseri with clinical significance.


Assuntos
Citrobacter koseri , Endoftalmite , Infecções por Enterobacteriaceae , Citrobacter koseri/genética , Endoftalmite/diagnóstico , Infecções por Enterobacteriaceae/diagnóstico , Humanos , Filogenia , Sequenciamento Completo do Genoma
3.
Surg Infect (Larchmt) ; 22(10): 1093-1096, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34379520

RESUMO

Background: A 69-year-old man underwent ligation and evacuation of a popliteal artery aneurysm with a femoral-to-popliteal vein bypass. He had a history of Citrobacter koseri prostatitis two months prior to the surgery. One month postoperatively, he presented with extremity swelling, redness, and fluid collections around the graft. Methods: A graft preserving strategy was adopted. The patient underwent operative drainage, washing, and received long-term antibiotic therapy. Fluid culture grew Citrobacter koseri, previously not reported as cause of surgical site infection with infrainguinal graft involvement. Results: The infection was treated successfully, and the patient is remains symptom free 18 months post-operatively. Conclusions: This case highlights the importance of considering culturing the aneurysm content in the presence of infectious history.


Assuntos
Aneurisma , Citrobacter koseri , Idoso , Artéria Femoral/cirurgia , Humanos , Masculino , Artéria Poplítea/diagnóstico por imagem , Artéria Poplítea/cirurgia , Infecção da Ferida Cirúrgica
4.
BMC Infect Dis ; 21(1): 600, 2021 Jun 23.
Artigo em Inglês | MEDLINE | ID: mdl-34162341

RESUMO

BACKGROUND: This antimicrobial surveillance study reports in vitro antimicrobial activity and susceptibility data for a panel of agents against respiratory isolates of Enterobacterales and Pseudomonas aeruginosa. METHODS: Isolates from respiratory specimens were collected in Africa/Middle East, Asia/South Pacific, Europe and Latin America between 2016 and 2018, as part of the Antimicrobial Testing Leadership and Surveillance (ATLAS) program. Broth microdilution methodology was used to quantify minimum inhibitory concentrations, from which rates of susceptibility were determined using EUCAST breakpoints (version 10). Rates of subsets with genes encoding ß-lactamases (extended-spectrum ß-lactamases [ESBLs], serine carbapenemases and metallo-ß-lactamases [MBLs]) were also determined, as well as rates of multidrug-resistant (MDR) P. aeruginosa. RESULTS: Among all respiratory Enterobacterales isolates, susceptibility to ceftazidime-avibactam, meropenem, colistin and amikacin was ≥94.4% in each region. For Enterobacterales isolates that were ESBL-positive or carbapenemase-positive/MBL-negative, ceftazidime-avibactam susceptibility was 93.6 and 98.9%, respectively. Fewer than 42.7% of MBL-positive Enterobacterales isolates were susceptible to any agents, except colistin (89.0% susceptible). Tigecycline susceptibility was ≥90.0% among Citrobacter koseri and Escherichia coli isolates, including all ß-lactamase-positive subsets. ESBL-positive Enterobacterales were more commonly identified in each region than isolates that were ESBL/carbapenemase-positive; carbapenemase-positive/MBL-negative; or MBL-positive. Among all respiratory P. aeruginosa isolates, the combined susceptibility rates (susceptible at standard dosing regimen plus susceptible at increased exposure) were highest to ceftazidime-avibactam, colistin and amikacin (≥82.4% in each region). Susceptibility to colistin was ≥98.1% for all ß-lactamase-positive subsets of P. aeruginosa. The lowest rates of antimicrobial susceptibility were observed among MBL-positive isolates of P. aeruginosa (≤56.6%), with the exception of colistin (100% susceptible). MDR P. aeruginosa were most frequently identified in each region (18.7-28.7%), compared with the subsets of ESBL-positive; carbapenemase-positive/MBL-negative; or MBL-positive isolates. CONCLUSIONS: Rates of susceptibility among the collections of respiratory Enterobacterales and P. aeruginosa isolates were highest to ceftazidime-avibactam, colistin and amikacin in each region. Tigecycline was active against all subsets of C. koseri and E. coli, and colistin was active against all subsets of P. aeruginosa. The findings of this study indicate the need for continued antimicrobial surveillance among respiratory Gram-negative pathogens, in particular those with genes encoding MBLs.


Assuntos
Antibacterianos/farmacologia , Enterobacteriaceae/efeitos dos fármacos , Enterobacteriaceae/isolamento & purificação , Pseudomonas aeruginosa/efeitos dos fármacos , Pseudomonas aeruginosa/isolamento & purificação , Amicacina/farmacologia , Compostos Azabicíclicos/farmacologia , Proteínas de Bactérias/genética , Ceftazidima/farmacologia , Citrobacter koseri/efeitos dos fármacos , Citrobacter koseri/isolamento & purificação , Colistina/farmacologia , Combinação de Medicamentos , Monitoramento Epidemiológico , Escherichia coli/efeitos dos fármacos , Escherichia coli/isolamento & purificação , Humanos , Meropeném/farmacologia , Testes de Sensibilidade Microbiana , Tigeciclina/farmacologia , beta-Lactamases/genética
5.
Antimicrob Agents Chemother ; 65(7): e0015021, 2021 06 17.
Artigo em Inglês | MEDLINE | ID: mdl-33972237

RESUMO

KPC-82 is a KPC-2 variant identified in a carbapenem-nonsusceptible Citrobacter koseri that confers high-level resistance to ceftazidime-avibactam. Genomic analysis revealed that blaKPC-82 is carried by a chromosomally integrated Tn4401 transposon (disrupting porin gene phoE) and evolved by a 6-nucleotide tandem repeat duplication causing a two-amino-acid insertion (Ser-Asp) within the Ala267-Ser275 loop. Similar to related KPC variants, KPC-82 showed decreased carbapenemase activity when expressed in a heterologous background and remained susceptible to carbapenem/ß-lactamase inhibitor combinations.


Assuntos
Carbapenêmicos , Citrobacter koseri , Antibacterianos/farmacologia , Compostos Azabicíclicos/farmacologia , Proteínas de Bactérias/genética , Carbapenêmicos/farmacologia , Ceftazidima/farmacologia , Combinação de Medicamentos , Klebsiella pneumoniae , Testes de Sensibilidade Microbiana , beta-Lactamases/genética
6.
J Med Microbiol ; 70(3)2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33439115

RESUMO

Introduction. Food allergies (FAs) occur due to intestinal immune dysfunction elicited by dysbiotic conditions. It was previously determined by us that Citrobacter species propagate in the faeces of mice with FAs and worsen allergic symptoms by inducing the allergenic cytokine IL-33. Dendritic cells can play important roles in regulation of FA responses.Hypothesis. Citrobacter species propagating in intestines of mice worsen allergic symptoms by stimulating dendritic cells to induce IL-33 expression.Aim. The aim of the present study was to analyse whether C. koseri stimulates dendritic cells to induce IL-33 expression.Methodology. IL-33 expression was evaluated in a DC2.4 mouse dendritic cell line stimulated by live or heat-inactivated C. koseri JCM1658, ATP, LPS extracted from C. koseri JCM1658 or other enterobacteria by real-time PCR. The ATP concentration and number of live bacteria in the culture supernatant were measured simultaneously.Results. Live C. koseri JCM1658 induced higher levels of IL-33 expression than other enterobacteria tested, but such a response was not elicited by heat-inactivated C. koseri JCM1658. LPS extracted from C. koseri JCM1658 did not induce IL-33 expression and suppressed live C. koseri JCM1658-induced IL-33 expression via the activation of Toll-like receptor 4 signalling. Furthermore, ATP produced by C. koseri JCM1658 stimulated dendritic cells to induce IL-33 expression by stimulating the P2X7 receptor, and LPS attenuated extracellular ATP-induced IL-33 expression. C. koseri JCM1658 was observed to proliferate more vigorously and produce more ATP than other enterobacteria.Conclusion. C. koseri acts as an allergenic bacterium through ATP production, stimulating dendritic cells to induce IL-33 expression, while LPS released from inactivated C. koseri JCM1658 attenuates this allergenicity.


Assuntos
Trifosfato de Adenosina/metabolismo , Citrobacter koseri/patogenicidade , Infecções por Enterobacteriaceae , Hipersensibilidade Alimentar , Interleucina-33/imunologia , Animais , Linhagem Celular , Células Dendríticas/microbiologia , Infecções por Enterobacteriaceae/imunologia , Infecções por Enterobacteriaceae/microbiologia , Hipersensibilidade Alimentar/imunologia , Hipersensibilidade Alimentar/microbiologia , Camundongos , Transdução de Sinais
7.
J Trop Pediatr ; 67(3)2021 07 02.
Artigo em Inglês | MEDLINE | ID: mdl-32594151

RESUMO

Since 2019 coronavirus disease (COVID-19) is highly contagious with a high mortality rate. France has taken strict infection control measures. According to the report by the Center for Disease Control and Prevention, children are less affected with COVID-19 and seem to have less severe disease than adults. We reported the first confirmed infant case of co-infection with SARS-CoV-2 and Citrobacter koseri urinary infection in 6-week-old child admitted on 25 March 2020 with mild symptoms in the Pediatric COVID Unit of Amiens University Hospital, France.


Assuntos
COVID-19 , Citrobacter koseri , Coinfecção , Humanos , Lactente , Controle de Infecções , SARS-CoV-2
9.
J Korean Med Sci ; 35(36): e303, 2020 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-32924341

RESUMO

BACKGROUND: Carbapenemase-producing Enterobacteriaceae (CPE) are emerging as a worldwide threat. Long-term care facilities (LTCFs) are considered a reservoir for CPE and play a central role in transmission to acute care hospitals. We investigated the CPE positivity in patients exposed to CPE in LTCFs. Furthermore, we analyzed the CPE positivity rates in the environment exposed to CPE. METHODS: We collected rectal swab specimens from patients residing in LTCFs who were exposed to CPE. Environmental sampling was performed by infection control practitioners from sites classified as patient private space, common space in the patient room, common space other than patient rooms, and nursing station. Each sample was cultured on a Chrom Klebsiella pneumoniae carbapenemase (KPC) agar for CPE screening. The positive isolates were subjected to a polymerase chain reaction to identify the presence of blaKPC, blaVIM, blaIMP, blaOXA-48, and blaNDM and determine CPE genotype. RESULTS: From 65 index cases, a total of 24 hospitals and 481 patients were enrolled; 414 patients who had resided in the same patient room as a patient with confirmed CPE and 67 patients who were newly admitted to that patient room. A total of 117 (24.3%) patients were positive for CPE among which 93 (22.5%, 93/414) were already admitted patients and 24 (35.8%, 24/67) were newly admitted patients. A total of 163 CPEs were detected and K. pneumoniae (n = 104, 63.8%) was the most common bacteria followed by Escherichia coli (n = 43, 26.4%) and Citrobacter koseri (n = 11, 6.7%). Environmental sampling was performed in 24 hospitals and 604 sites. A total of 12 sites (2.0%) were positive for CPE and sink in the nursing station (n = 6, 4.2%) was the most contaminated space. CONCLUSION: CPE colonization rates in patients exposed to CPE in LTCFs were higher than those found in acute care hospitals. Proper infection control measures for detecting and reducing CPE colonization in patients residing in LTCFs are required. Newly admitted patients could also be carriers; therefore, infection control for newly admitted patients also needs to be thorough.


Assuntos
Enterobacteriáceas Resistentes a Carbapenêmicos/isolamento & purificação , Infecções por Enterobacteriaceae/diagnóstico , Citrobacter koseri/isolamento & purificação , Infecções por Enterobacteriaceae/microbiologia , Escherichia coli/isolamento & purificação , Hospitais , Humanos , Klebsiella pneumoniae/isolamento & purificação , Assistência de Longa Duração , Reto/microbiologia , República da Coreia , Estudos Retrospectivos , Seul
10.
Bioprocess Biosyst Eng ; 43(9): 1599-1607, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32333194

RESUMO

Transaminase responsible for alienating prochiral ketone compound is applicable to asymmetric synthesis of herbicide L-phosphinothricin (L-PPT). In this work, the covalent immobilization of recombinant transaminase from Citrobacter koseri (CkTA) was investigated on different epoxy resins. Using optimum ES-105 support, a higher immobilized activity was obtained via optimizing immobilization process in terms of enzyme loading, coupling time and initial PLP concentration. Crucially, due to blocking unreacted epoxy groups on support surface with amino acids, the reaction temperature of blocked immobilized biocatalyst was enhanced from 37 to 57 °C. Its thermostability at 57 °C was also found to be superior to that of free CkTA. The Km value was shifted from 36.75 mM of free CkTA to 39.87 mM of blocked immobilized biocatalyst, demonstrating that the affinity of enzyme to the substrate has not been apparently altered. Accordingly, the biocatalyst performed the consecutive synthesis of L-PPT for 11 cycles (yields>91%) with retaining more than 91.13% of the initial activity. The seemingly the highest reusability demonstrates this biocatalyst has prospective for reducing the costs of consecutive synthesis of L-PPT with high conversion.


Assuntos
Aminobutiratos/síntese química , Proteínas de Bactérias/química , Citrobacter koseri/enzimologia , Enzimas Imobilizadas/química , Resinas Epóxi/química , Transaminases/química , Proteínas de Bactérias/genética , Citrobacter koseri/genética , Enzimas Imobilizadas/genética , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Transaminases/genética
11.
Braz J Microbiol ; 51(3): 969-977, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32291739

RESUMO

Currently, poultry farming is one of the sectors that have a significant impact on the global economy. In recent years, there has been an increase in the production of broilers, inflicting this segment of the industry to generate tons of keratin due to huge disposal of chicken feathers. This points to the need to degrade these chicken feathers, as they have emerged as a major threat to the environment. Thus, in this study we aimed to identify keratinases that are produced by the bacterium Citrobacter diversus and further investigate the biochemical characteristics of these keratin-degrading enzymes. In a submerged medium, the bacterium was capable of degrading chicken feathers almost completely after 36 h of fermentation. We found a maximum caseinolytic activity at pH 9-10.5 and 50-55 °C, and keratinolytic activity at pH 8.5-9.5 and 50 °C. Thus, given its stability at higher temperatures, upon incubation of this enzyme extract for 1 h at 50 °C, it showed approximately 50% of the keratinolytic and 100% of the caseinolytic activity. Further, under pH stability for 48 h at 4 °C, the enzyme extract maintained greater residual activity in the pH range 6-8. Caseinolytic activity was inhibited by EDTA and PMSF, whereas the keratinolytic activity was inhibited only by EDTA. Additionally, due to its alkaline activity and detergent compatibility, this enzyme extract could improve washing performance when added to a commercial detergent formulation. Using application tests, we could demonstrate a potential use of this bacterial enzyme extract as an additive in detergents to remove egg stains from cloth.


Assuntos
Proteínas de Bactérias/metabolismo , Citrobacter koseri/enzimologia , Detergentes/metabolismo , Peptídeo Hidrolases/metabolismo , Animais , Proteínas de Bactérias/isolamento & purificação , Biodegradação Ambiental , Caseínas/metabolismo , Galinhas , Citrobacter koseri/metabolismo , Meios de Cultura/metabolismo , Detergentes/química , Plumas/metabolismo , Fermentação , Concentração de Íons de Hidrogênio , Queratinas/metabolismo , Peptídeo Hidrolases/isolamento & purificação , Temperatura
12.
Bioprocess Biosyst Eng ; 43(4): 637-644, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31865453

RESUMO

The biocatalyzed synthesis of purine nucleosides and their analogs is a case widely studied due to the high pharmaceutical interest of these compounds, providing the whole-cell biocatalysts, a useful tool for this purpose. Vidarabine and fludarabine are commercial examples of expensive bioactive nucleosides that can be prepared using a microbial transglycosylation approach. Citrobacter koseri whole-cells immobilized on agarose beads proved to be an interesting option to transform this biotransformation in a preparative process. The entrapment matrix provided a useful and resistant multipurpose biocatalyst regarding its stability, mechanical strength, microbial viability and reuse. Immobilized biocatalyst retained the initial activity for up to 1 year storage and after 10 years, the biocatalyst did not show cell leaking and still exhibited residual activity. In addition, the biocatalyst could be reused in batch 68 times keeping up to 50% of the initial biocatalytic activity and for at least 124 h in a continuous process.


Assuntos
Biocatálise , Células Imobilizadas/metabolismo , Citrobacter koseri/metabolismo , Nucleosídeos/biossíntese , Sefarose/química , Células Imobilizadas/citologia , Citrobacter koseri/citologia
13.
Transfusion ; 60(2): 245-249, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-31804713

RESUMO

BACKGROUND: Transfusion-transmitted bacterial infection is a rare occurrence but the most feared complication in transfusion practices. Between 2012 and 2017, five cases of platelet concentrates (PCs) contaminated with the bacterial pathogen Citrobacter koseri (PC-Ck) have been reported in France, with two leading to the death of the recipients. We tested the possibilities of the emergence of a PC-specific clone of C. koseri (Ck) and of specific bacterial genes associated with PC contamination. STUDY DESIGN AND METHODS: The phylogenetic network, based on a homemade Ck core genome scheme, inferred from the genomes of 20 worldwide Ck isolates unrelated to PC contamination taken as controls (U-Ck) and the genomes of the five PC-Ck, explored the clonal relationship between the genomes and evaluated the distribution of PC-Ck throughout the species. Along with this core genome multilocus sequence typing approach, a Ck pan genome has been used to seek genes specific to PC-Ck isolates. RESULTS: Our genomic approach suggested that the population of C. koseri is nonclonal, although it also identified a cluster containing three PC-Ck and eight U-Ck. Indeed, the PC-Ck did not share any specific genes. CONCLUSION: The elevated incidence of PCs contaminated by C. koseri in France between 2012 and 2017 was not due to the dissemination of a clone. The determinants of the recent outbreaks of PC contamination with C. koseri are still unknown.


Assuntos
Citrobacter koseri/genética , Antibacterianos/farmacologia , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Citrobacter koseri/efeitos dos fármacos , França , Genótipo , Humanos , Filogenia
14.
Clin Med (Lond) ; 20(1): 92-94, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31704728

RESUMO

BACKGROUND: Isolated aortic infection is rare and is typically associated with an underlying aortic aneurysm or a prosthetic aortic graft. CASE SUMMARY: A 38-year-old woman was admitted with symptoms of left upper limb ischaemia and had imaging findings suggestive of thrombus extending from the ascending aorta into the subclavian and brachial arteries. She underwent evacuation of the aortic masses and replacement of the ascending aorta. Citrobacter koseri was isolated from the excised tissue and the patient received 6 weeks of appropriate antibiotic therapy. DISCUSSION: This is an unusual case of acute upper limb ischaemia due to a mass infected with Citrobacter koseri in the ascending aorta without heart valve involvement.


Assuntos
Arteriopatias Oclusivas , Citrobacter koseri , Trombose , Adulto , Antibacterianos/uso terapêutico , Aorta , Feminino , Humanos
15.
J Glob Antimicrob Resist ; 20: 94-97, 2020 03.
Artigo em Inglês | MEDLINE | ID: mdl-31323426

RESUMO

OBJECTIVES: This study reported the resistome content of sewage sludge-isolated carbapenem-resistant Citrobacter koseri (C. koseri) carrying blaOXA-181. It also provided a general phylogenomic analysis highlighting antibiotic resistance genes (ARGs), plasmids and pathogenicity of C. koseri genomes. METHODS: The carbapenem-resistantC. koseri AS1 strain was isolated from sewage sludge on CHROMagar™ mSuperCARBA™ media. Whole genome sequencing of C. koseri AS1 was performed using an HiSeq X™ Ten instrument. Additional C. koseri genomes were downloaded from National Center for Biotechnology Information (NCBI). Phylogenomic analysis was established through CSI Phylogeny. ARGs, plasmids and pathogenicity were identified using ResFinder 3.1, PlasmidFinder 2.0 and PathogenFinder 1.1, respectively. RESULTS: The phylogenomic tree indicated a polyclonal pattern ofC. koseri genomes. Resistome analysis of C. koseri AS1 revealed ß-lactam resistance genes (blaMAL-1 and blaOXA-181) as well as a fosfomycin resistance gene (fosA7). Three plasmids (ColKP3, ColRNAI and IncX30) were identified in the C. koseri AS1 genome. In addition, 25 ARGs were found in downloaded genomes. Of these, clinically significant ARGs such as blaKPC-2 and blaOXA-48 were found in two and four genomes, respectively. Assessment of the genomes using PathogenFinder revealed all genomes as putative human pathogens. CONCLUSIONS: It is believed that noC. koseri genome has been reported to carry blaOXA-181; therefore, C. koseri AS1 is the first of its kind. This study also highlighted the resistome contents of C. koseri genomes.


Assuntos
Carbapenêmicos/farmacologia , Citrobacter koseri/classificação , Esgotos/microbiologia , Sequenciamento Completo do Genoma/métodos , beta-Lactamases/genética , Proteínas de Bactérias/genética , Citrobacter koseri/efeitos dos fármacos , Citrobacter koseri/genética , Citrobacter koseri/isolamento & purificação , Farmacorresistência Bacteriana Múltipla , Fosfomicina/farmacologia , Tamanho do Genoma , Genoma Bacteriano , Sequenciamento de Nucleotídeos em Larga Escala , Testes de Sensibilidade Microbiana , Filogenia , Plasmídeos/genética
16.
Retin Cases Brief Rep ; 14(2): 187-191, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-29155695

RESUMO

PURPOSE: We present a case of endogenous endophthalmitis because of an unusual bacterium, Citrobacter koseri. PATIENT: A 57-year-old woman without previous history of eye surgery or trauma presented with diabetic ketoacidosis and a painful right eye with the reduction of vision. C. koseri was identified in blood culture; thus, a diagnosis of right eye endogenous endophthalmitis was made. Intravenous and intravitreal antibiotics were both started, and vitreous culture further confirmed C. koseri as the causative organism. Computed tomography of the abdomen and pelvis revealed a right C-shaped perinephric abscess, which was drained under ultrasound guidance. RESULTS: Because of rapid progression to corneal melting, evisceration was performed. CONCLUSION: Cases of endogenous endophthalmitis caused by Citrobacter are very limited, and a review of all published cases in the English literature and the present case revealed that endogenous Citrobacter endophthalmitis arose almost entirely from Citrobacter renal infection. Early recognition and drainage of renal abscess may lower the chance of uncontrolled infection and endogenous spread to the eyes. Despite prompt and intensive treatment, the clinical outcome of Citrobacter endogenous endophthalmitis seems to be poor.


Assuntos
Citrobacter koseri/isolamento & purificação , Endoftalmite/microbiologia , Infecções por Enterobacteriaceae/microbiologia , Infecções Oculares Bacterianas/microbiologia , Acuidade Visual , Corpo Vítreo/microbiologia , Endoftalmite/diagnóstico , Infecções por Enterobacteriaceae/diagnóstico , Infecções Oculares Bacterianas/diagnóstico , Feminino , Humanos , Pessoa de Meia-Idade , Ultrassonografia , Corpo Vítreo/diagnóstico por imagem
17.
Br J Neurosurg ; 34(1): 94-95, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29235359

RESUMO

A 60-year-old female had a frontal bone intraosseous meningioma resected 10 years previously. On follow up CT head, an enlarging intraosseous frontal bone lesion was noted. This was thought to be a recurrent frontal meningioma. Intraooperatively, she was found to have an abscess deep to the cranioplasty.


Assuntos
Encefalopatias/microbiologia , Infecções do Sistema Nervoso Central/microbiologia , Citrobacter koseri , Infecções por Enterobacteriaceae/microbiologia , Abscesso/microbiologia , Abscesso/cirurgia , Encefalopatias/diagnóstico por imagem , Infecções do Sistema Nervoso Central/diagnóstico por imagem , Infecções por Enterobacteriaceae/diagnóstico por imagem , Feminino , Humanos , Meningioma/cirurgia , Pessoa de Meia-Idade , Infecção da Ferida Cirúrgica/microbiologia , Infecção da Ferida Cirúrgica/cirurgia , Tomografia Computadorizada por Raios X
18.
Molecules ; 24(24)2019 Dec 13.
Artigo em Inglês | MEDLINE | ID: mdl-31847159

RESUMO

In this study, we determined the antimicrobial activity of ten essential oils (EOs)-oregano, thyme, clove, arborvitae, cassia, lemongrass, melaleuca, eucalyptus, lavender, and clary sage-against drug-resistant microorganisms previously isolated from patients with skin infections. The essential oil compositions were determined using gas chromatography coupled to mass spectrometry (GC/MS). The assayed bacteria included Pseudomonas aeruginosa, Proteus vulgaris, Citrobacter koseri, and Klebsiella pneumoniae. Two drug-resistant yeasts (Candida albicans and Candida parapsilosis) were also involved in our survey. Oregano, thyme, cassia, lemongrass and arborvitae showed very strong antibacterial and antifungal activity against all tested strains. These results show that these essential oils may be effective in preventing the growth of the drug-resistant microorganisms responsible for wound infections. In this study, the genotoxic effects of tested essential oils on healthy human keratinocytes HaCaT were evaluated using the comet assay for the first time. These results revealed that none of the essential oils induced significant DNA damage in vitro after 24 h. Moreover, the treatment of HaCaT cells with essential oils increased the total antioxidant status (TAS) level. The obtained results indicate that EOs could be used as a potential source of safe and potent natural antimicrobial and antioxidant agents in the pharmaceutical and food industries.


Assuntos
Farmacorresistência Bacteriana/efeitos dos fármacos , Farmacorresistência Fúngica/efeitos dos fármacos , Óleos Voláteis/química , Óleos Vegetais/química , Dermatopatias Infecciosas/microbiologia , Candida albicans/efeitos dos fármacos , Candida parapsilosis/efeitos dos fármacos , Cassia/química , Linhagem Celular , Citrobacter koseri/efeitos dos fármacos , Cymbopogon/química , Humanos , Klebsiella pneumoniae/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Óleos Voláteis/farmacologia , Origanum/química , Óleos Vegetais/farmacologia , Proteus vulgaris/efeitos dos fármacos , Pseudomonas aeruginosa/efeitos dos fármacos , Thuja/química , Thymus (Planta)/química
19.
Biochem Biophys Res Commun ; 517(3): 427-432, 2019 09 24.
Artigo em Inglês | MEDLINE | ID: mdl-31376937

RESUMO

Citrobacter species are opportunistic bacterial pathogens that are implicated in both nosocomial and community-acquired infections. Among the Citrobacter species, Citrobacter koseri is often isolated from clinical material, and it can cause meningitis and brain abscesses in neonates and immunocompromised individuals, thus posing a great threat to human health. However, the virulence determinants of C. koseri remain largely unknown. Myo-inositol is an abundant carbohydrate in the environment and in certain organs of the human body, especially the brain. The C. koseri genome harbors a cluster of genes, QCQ70420.1 to QCQ70429.1 (named the Ino-cluster in this study), which encode IolBCDE, MmsA, and an ATP-binding cassette transporter. The gene cluster may be involved in the utilization of myo-inositol. To investigate the functions of the Ino-cluster in C. koseri, we constructed a mutant strain by deleting the Ino-cluster and found that the mutant could not use myo-inositol as the sole carbon source, confirming that this cluster is responsible for myo-inositol utilization. Moreover, we investigated the function of the Ino-cluster and myo-inositol utilization in C. koseri pathogenicity. Deletion of the Ino-cluster significantly impaired C. koseri colonization of the brain of infected Sprague-Dawley (SD) rats and BALB/c mice, and this increased the survival rate of the infected animals, indicating that the Ino-cluster and the ability to use myo-inositol are essential for C. koseri pathogenicity. Taken together, our findings suggest that using the Ino-cluster products, C. koseri can exploit the abundant myo-inositol in the brain as a carbon source for growth, thus promoting colonization and virulence.


Assuntos
Proteínas de Bactérias/genética , Citrobacter koseri/metabolismo , Citrobacter koseri/patogenicidade , Infecções por Enterobacteriaceae/microbiologia , Genes Bacterianos , Inositol/metabolismo , Animais , Proteínas de Bactérias/metabolismo , Transporte Biológico , Encéfalo/metabolismo , Encéfalo/microbiologia , Encéfalo/patologia , Citrobacter koseri/genética , Citrobacter koseri/crescimento & desenvolvimento , Modelos Animais de Doenças , Infecções por Enterobacteriaceae/metabolismo , Infecções por Enterobacteriaceae/mortalidade , Infecções por Enterobacteriaceae/patologia , Deleção de Genes , Expressão Gênica , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Família Multigênica , Ratos , Ratos Sprague-Dawley , Análise de Sobrevida , Virulência
20.
BMJ Case Rep ; 12(7)2019 Jul 27.
Artigo em Inglês | MEDLINE | ID: mdl-31352398

RESUMO

We present an elderly diabetic man with left hallux pain and drainage who was initially diagnosed with acute gouty arthritis using the diagnostic rule for acute gout and monosodium urate crystals presented on synovial fluid analysis. Further investigation with surgical debridement, plain X-ray, MRI and wound culture revealed concomitant Citrobacter koseri septic arthritis with osteomyelitis. C. koseri is considered an opportunistic infection that rarely causes musculoskeletal infections. Acute gouty arthritis and septic arthritis are rarely seen occurring concomitantly in the same joint and are often difficult to differentiate due to similar findings on exam and imaging. The present case illustrates that osteomyelitis with an opportunistic organism can present concomitantly with acute gouty arthritis, and the diagnosis of one should not exclude the other.


Assuntos
Antibacterianos/uso terapêutico , Artrite Gotosa/microbiologia , Citrobacter koseri/patogenicidade , Pé Diabético/fisiopatologia , Infecções por Enterobacteriaceae/diagnóstico , Ertapenem/uso terapêutico , Osteomielite/diagnóstico , Idoso de 80 Anos ou mais , Artrite Gotosa/terapia , Desbridamento , Pé Diabético/microbiologia , Infecções por Enterobacteriaceae/fisiopatologia , Infecções por Enterobacteriaceae/terapia , Humanos , Masculino , Osteomielite/fisiopatologia , Osteomielite/terapia , Combinação Piperacilina e Tazobactam/uso terapêutico , Resultado do Tratamento , Vancomicina/uso terapêutico
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