Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 65
Filtrar
Mais filtros










Intervalo de ano de publicação
1.
Appl Biochem Biotechnol ; 193(4): 1215-1230, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33200266

RESUMO

This study evaluated the potential of dwarf morning-glory Convolvulus tricolor (Convolvulaceae) plants and their hairy roots induced by Agrobacterium rhizogenes for rhizoextraction of heavy metals ions from the liquid medium under aseptic growth conditions. Both the young C. tricolor plants and the generated hairy root culture efficiently removed Cr6+, Ni2+, and Pb2+ ions from the liquid cultivation medium. As determined by atomic absorption spectroscopy, the hairy roots demonstrated a high level of heavy metal ions accumulation (µg/g dry weight): 3942 ± 210 of chromium, 1529 ± 312 of nickel, and 2613 ± 373 of lead. These data show that the hairy roots of morning glory might be of interest for some phytoremediation strategies due to their high bioaccumulation abilities. The comparison of bioaccumulation potential of established hairy roots and young C. tricolor plants give grounds to suppose that roots of C. tricolor play an active role in the absorption of Cr6+, Ni2+, and Pb2+ from liquid media, whereas the aboveground part rather serves as a storage for the accumulated metal ions.


Assuntos
Cromo/metabolismo , Convolvulus/crescimento & desenvolvimento , Chumbo/metabolismo , Níquel/metabolismo , Raízes de Plantas/crescimento & desenvolvimento , Agrobacterium , Biodegradação Ambiental
2.
Phytochemistry ; 182: 112594, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33341029

RESUMO

The scopoletin one of the major bioactive components of Convolvulus prostratus Forssk known to have a role in acetylcholinesterase inhibitor, memory enhancer, antimicrobial, antioxidative etc. properties are investigated in the present study. The concentration of scopoletin in C. prostratus is investigated in leaf, stem and root at different growth stages of plant development viz., 30, 45, 60 and 90 days after sowing (DAS). A highly sensitive LC-MS method was developed to quantify the scopoletin even at low concentration with LOD and LOQ of 8 and 24 ng/ml, respectively. The highest quantity of scopoletin was recorded in stem (732 µg/g dry weight) and leaf (650 µg/g dry weight) collected 90 DAS whereas lowest was recorded at 45 DAS in leaf (90.00 µg/g dry weight) and Stem (110 µg/g dry weight). Based on the highest and lowest concentration of scopoletin in stem and root tissues at 45 and 90 DAS were selected for transcriptome study. Differential gene expression analysis revealed the differential expression of genes involved in scopoletin biosynthesis. Seven genes viz., phenylalanine ammonia-lyase (PAL), 4-coumarate CoA ligase (4CL), trans-cinnamate 4-monooxygenase (TCM), shikimate O- hydroxycinnamoyl transferase (C3'H), 5-O-4-coumaroyl-D-quinate 3'-monooxygenase (HCT), caffeoyl-CoA-O-methyltransferase (CCoAOMT) and feruloyl-CoA 6'-hydroxylase (F6'H) were identified in the phenyl propanoid pathway. Expression of the novel enzyme F6'H showed down regulation in both tissues at 45 DAS. Real-time PCR showed a correlation with the expression of this F6'H genes with the accumulation of scopoletin at 90 DAS. This indicated that the growth stage of plant and expression of F6'H control the scopoletin accumulation in Convolvulus. The results of present investigation may useful in pharmaceutical, drug and cosmetic industries that the harvesting of plant part especially stem of C.prostratus at 90 DAS to get maximum quantity of scopoletin. Also, the novel gene F6'H need to be further characterized to understand its expression dynamics so that scopoletin content can be increase at the highest.


Assuntos
Convolvulus , Escopoletina , Regulação da Expressão Gênica de Plantas , Fenilalanina Amônia-Liase/genética , Folhas de Planta , Transcinamato 4-Mono-Oxigenase
3.
J Photochem Photobiol B ; 209: 111949, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32659646

RESUMO

Scientists are interested in biosynthesis of gold nanoparticles owing to their catalytic and biological features. In this research, the extract of Convolvulus fruticosus (C. fruticosus; CFE) extract was applied to synthesize spherical-like gold nanoparticles (CFE@AuNPs). As-prepared CFE@AuNPs was characterized by TEM, FE-SEM, XRD, FT-IR, EDS, UV-Vis and DLS analysis. Identification analysis revealed that the properties of as-prepared CFE@AuNPs with spherical morphology were homogeneous, regular, high dispersibility and low agglomeration. The particle size of biogenic gold nanoparticles (about 35 nm) was obtained using FE-SEM, TEM and DLS techniques. Photocatalytic experiment of CFE@AuNPs determined by degradation of basic violet 10 (BV10), basic blue 9 (BB9) and acid red 51 (AR51) pollutants with percent degradation of 94.3%, 90.2%, 85.4% under UV and 80.6%, 79.8%, 73.3% under visible light irradiation, respectively. As well as, as-prepared CFE@AuNPs illustrated as a significant inhibitory influence against ATCC strain for both gram-positive and gram-negative bacteria. Owing to the antibacterial results, CFE@AuNPs enhanced antibacterial activity against E. coli, A. baumannii, P. aeruginosa, S. aureus, K. pneumonia, P. mirabilis and E. faecalis with MIC of 0.075, 0.075, 0.075, 0.075, 0.15, 0.075 and 0.037 mg/ml, respectively. All in all, results of the findings showed that C. fruticosus capped gold nanoparticles can find applications in the various arena including biological and removal of toxic pollutants for water purification.


Assuntos
Antibacterianos/farmacologia , Convolvulus/química , Ouro/química , Nanopartículas Metálicas/química , Extratos Vegetais/farmacologia , Catálise , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Positivas/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Microscopia Eletrônica de Varredura , Microscopia Eletrônica de Transmissão , Análise Espectral/métodos , Temperatura , Difração de Raios X
4.
Neuroreport ; 31(8): 597-604, 2020 05 22.
Artigo em Inglês | MEDLINE | ID: mdl-32282574

RESUMO

The memory-boosting property of Indian traditional herb, Convolvulus pluricaulis, has been documented in literature; however, its effect on synaptic plasticity has not yet been reported. Two important forms of synaptic plasticity known to be involved in the processes of memory formation are long-term potentiation (LTP) and long-term depression (LTD). In the present study, the effect of C. pluricaulis plant extract on LTP and LTD were evaluated. The adult male Wistar rats were fed orally with 250, 500 and 1000 mg/kg of this extract for 4 weeks and the effect was determined on LTP and LTD in the Schaffer collaterals of the hippocampal cornu ammonis region CA1. We found that the 500 mg/kg dose of the extract could significantly enhance LTP compared to the vehicle treated ones. Moreover, the same dose could also reduce LTD while used in a separate set of animals. Also, a fresh group of animals treated with the effective dose (500 mg/kg) of plant extract were examined for memory retention in two behavioral platforms namely, contextual fear conditioning (CFC) and novel object recognition test (NORT). Increased fear response to the conditioned stimulus and enhanced recognition of objects were observed in CFC and NORT, respectively, both indicating strengthening of memory. Following up, ex-vivo electrophysiology experiments were performed with the active single molecule scopoletin, present in C. pluricaulis extract and similar patterns in synaptic plasticity changes were obtained. These findings suggest that prolonged treatment of C. pluricaulis extract, at a specific dose in healthy animals, can augment memory functions by modulating hippocampal plasticity.


Assuntos
Convolvulus , Hipocampo/efeitos dos fármacos , Hipocampo/fisiologia , Potenciação de Longa Duração/efeitos dos fármacos , Depressão Sináptica de Longo Prazo/efeitos dos fármacos , Extratos Vegetais/administração & dosagem , Animais , Condicionamento Clássico/efeitos dos fármacos , Condicionamento Clássico/fisiologia , Masculino , Memória/efeitos dos fármacos , Memória/fisiologia , Ratos Wistar
5.
J Ethnopharmacol ; 249: 112393, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31743764

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Convolvulus pluricaulis Choisy commonly known as Shankhapushpi, is traditionally prescribed for nerve debility, loss of memory, epilepsy and as nervine tonic. Plant also proved to have diverse pharmacological activity but the neuroprotection in ischemic stroke were not found. AIM OF THE STUDY: To investigate the effect of Convolvulus pluricaulis against bilateral common carotid artery (BCCA) occlusion induced cerebral ischemic reperfusion injury. MATERIALS AND METHODS: The neuroprotective activity of Convolvulus pluricaulis against bilateral common carotid artery (BCCA) occlusion induced cerebral ischemic reperfusion (I/S) injury. Sprague-Dawley rats of either sex (200-250 g) were divided into nine groups of 8 rats each. Sham and control group, saline treated 10 ml/kg orally. Third group treated with Quercetin 25 mg/kg orally and fourth to ninth groups treated with chloroform and ethanol extract of Convolvulus pluricaulis 100, 200, and 400 mg/kg (p.o.) respectively. Control, Quercetin and extract treated groups underwent 30 min BCCA occlusion and 24 h reperfusion on 10th day but sham underwent same surgery without BCCA occlusion and 24 h reperfusion on 10th day. The antioxidant enzymatic and non-enzymatic levels were estimated by UV spectroscopic method and cerebral infarction area, Blood brain barrier disruption, microtubule-associated protein 2 immunohistochemical and histopathological studies were carried out. RESULTS: The results of the study indicate that the chloroform and ethanol extract of Convolvulus pluricaulis showed neuroprotective activity by a significant decrease in lipid peroxidation (p < 0.001) and an increase in superoxide dismutase (p < 0.01, p < 0.001), catalase (p < 0.01, p < 0.001), glutathione (p < 0.001), and total thiol (p < 0.001) levels in extract-treated groups as compared to control group. Measurement of cerebral infarction area, blood brain barrier disruption, microtubule-associated protein 2 immunohistochemical and histopathological studies further supported the protective effect of the extract. CONCLUSIONS: Present study revile that Convolvulus pluricaulis has potent neuroprotection against bilateral common carotid artery (BCCA) occlusion induced cerebral ischemic reperfusion injury.


Assuntos
Convolvulus/química , Fármacos Neuroprotetores/farmacologia , Extratos Vegetais/farmacologia , Traumatismo por Reperfusão/tratamento farmacológico , Animais , Antioxidantes/metabolismo , Isquemia Encefálica/prevenção & controle , Infarto Cerebral/tratamento farmacológico , Relação Dose-Resposta a Droga , Feminino , Masculino , Proteínas Associadas aos Microtúbulos/metabolismo , Fármacos Neuroprotetores/administração & dosagem , Fármacos Neuroprotetores/isolamento & purificação , Estresse Oxidativo/efeitos dos fármacos , Extratos Vegetais/administração & dosagem , Quercetina/farmacologia , Ratos , Ratos Sprague-Dawley , Traumatismo por Reperfusão/patologia , Acidente Vascular Cerebral/prevenção & controle
6.
Phytother Res ; 34(2): 315-328, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31713286

RESUMO

Convolvulus genus is a representative of the family of Convolvulaceae. Convolvulus plants are broadly distributed all over the world and has been used for many centuries as herbal medicine. Convolvulus genus contains various phytochemicals such as flavonoids, alkaloids, carbohydrates, phenolic compounds, mucilage, unsaturated sterols or terpenes, resin, tannins, lactones, and proteins. This review highlights the phytochemical composition, antimicrobial and antioxidant activities, application as food preservative, traditional medicine use, anticancer activities, and clinical effectiveness in human of Convolvulus plants. All the parts of Convolvulus plants possess therapeutic benefits; preliminary pharmacological data validated their use in traditional medicine. However, further preclinical and clinical experiments are warranted before any application in human health.


Assuntos
Convolvulus/química , Compostos Fitoquímicos/farmacologia , Extratos Vegetais/farmacologia , Plantas Medicinais/química , Animais , Anti-Infecciosos/farmacologia , Antineoplásicos Fitogênicos/farmacologia , Antioxidantes/farmacologia , Convolvulus/classificação , Conservantes de Alimentos , Humanos , Medicina Tradicional , Fitoterapia , Plantas Medicinais/classificação
7.
J Chromatogr Sci ; 57(10): 920-930, 2020 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-31644789

RESUMO

The use of herbs as medicine is an ancient form of healthcare known to mankind. Standardization of herbal medicines is however a challenging task and is the major bottleneck in their acceptance as the primary therapeutic option. The aim of this study was to develop and validate a simple, rapid HPLC method for standardizing the mixture of extracts of three Medhya Rasayanas (neurotonic), Convolvulus pluricaulis, Withania somnifera and Bacopa monnieri. Simultaneous estimation of the respective bioactive markers of these plants viz., scopoletin, withaferin A, bacoside A 3, bacopaside II, jujubogenin and bacosaponin C has been reported for the first time. The method was developed using Waters Hybrid X-Bridge shield with BEH technology 2.5 µm, 4.6 × 75 mm column and validated according to ICH guidelines. The 20 minutes run time makes the method eco-friendly. The method was linear over a range of 12.5-400 ng/10 µL for scopoletin and 62.5-2,000 ng/10 µL for withaferin A, bacoside A 3, bacopaside II, jujubogenin and bacosaponin C with detection limits of 8.0, 48.3, 30.4, 40.7, 15.6 and 18.9 ng/10 µL and quantification limits of 24.5, 146.5, 92.2, 123.4, 47.4 and 57.4 ng/10 µL, respectively. The correlation coefficient for each analyte was >0.999. The intra-day and inter-day precision was <2%. These results confirmed the precision, accuracy and robustness of the proposed method.


Assuntos
Bacopa/química , Cromatografia Líquida de Alta Pressão/métodos , Convolvulus/química , Extratos Vegetais/análise , Withania/química , Biomarcadores/análise , Limite de Detecção , Modelos Lineares , Extratos Vegetais/química , Reprodutibilidade dos Testes , Escopoletina/análise , Triterpenos/análise , Vitanolídeos/análise
8.
Nat Prod Res ; 34(10): 1490-1493, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-30518259

RESUMO

Convolvulus trabutianus Schweinf. & Muschl. is an endemic plant from northern Sahara used in folk medicine. Herein we report, the isolation, characterization and evaluation of the radical scavenging properties of twenty three compounds from different extracts of this species by on-line HPLC-ABTS•+ screening. These compounds include nine phenolic acids: 2, 6, 10-16, two phytosterols: 3-4, four coumarins: 5, 7-9, two quinic acids: 21 and 22 and six flavonoids: 1, 17-20 and 23 among which the most active were: 10, 16, 21 and 22. All the extracts showed a significant antioxidant activity on-line. These results were validated off-line by ORAC and TEAC assays. Four compounds: 1, 5, 18 and 19 were described for the first time from the Convolvulaceae family, whereas compounds 2, 6, 8, 10, 13 and 21 were new for the genus Convolvulus.


Assuntos
Antioxidantes/farmacologia , Convolvulus/química , Avaliação Pré-Clínica de Medicamentos/métodos , Polifenóis/farmacologia , África do Norte , Antioxidantes/isolamento & purificação , Cromatografia Líquida de Alta Pressão/métodos , Flavonoides/análise , Flavonoides/isolamento & purificação , Hidroxibenzoatos , Extratos Vegetais/química , Plantas Medicinais/química , Polifenóis/isolamento & purificação , Ácido Quínico/isolamento & purificação
9.
Sci Rep ; 9(1): 8133, 2019 05 31.
Artigo em Inglês | MEDLINE | ID: mdl-31148556

RESUMO

Convolvulus arvensis is a troublesome weed that is naturally tolerant to glyphosate. This weed tolerates glyphosate at a rate 5.1 times higher than that of glyphosate-susceptible Calystegia hederacea. Glyphosate-treated C. arvensis plants accumulated less shikimic acid than C. hederacea plants. The overexpression of EPSPS genes from the two species in transgenic Arabidopsis thaliana resulted in similar glyphosate tolerance levels. qPCR of genomic DNA revealed that the EPSPS copy number in C. arvensis was approximately 2 times higher than that in C. hederacea. Moreover, glyphosate treatment caused a marked increase in EPSPS mRNA in C. arvensis compared to C. hederacea. GUS activity analysis showed that the promoter of CaEPSPS (CaEPSPS-P) highly improved GUS expression after glyphosate treatment, while no obvious differential GUS expression was observed in ChEPSPS-P transgenic A. thaliana in the presence or absence of glyphosate. Based on the obtained results, two coexisting mechanisms may explain the natural glyphosate tolerance in C. arvensis: (i) high EPSPS copy number and (ii) specific promoter-mediated overexpression of EPSPS after glyphosate treatment.


Assuntos
3-Fosfoshikimato 1-Carboxiviniltransferase/genética , Arabidopsis/efeitos dos fármacos , Calystegia/efeitos dos fármacos , Convolvulus/efeitos dos fármacos , Regulação da Expressão Gênica de Plantas , Glicina/análogos & derivados , 3-Fosfoshikimato 1-Carboxiviniltransferase/biossíntese , Arabidopsis/enzimologia , Bioensaio , Calystegia/enzimologia , Convolvulus/enzimologia , Bases de Dados Genéticas , Relação Dose-Resposta a Droga , Tolerância a Medicamentos , Glicina/química , Resistência a Herbicidas/genética , Herbicidas/química , Plantas Geneticamente Modificadas , Reação em Cadeia da Polimerase , Pós , Regiões Promotoras Genéticas , Ácido Chiquímico/metabolismo
10.
J Nat Prod ; 82(6): 1593-1598, 2019 06 28.
Artigo em Inglês | MEDLINE | ID: mdl-31181918

RESUMO

Six new glycosidic acids, arvensic acids E-J (1-6), were obtained from a glycosidic acid fraction afforded by alkaline hydrolysis of the crude resin glycosides from Convolvulus arvensis whole plants. Their structures were established from the spectroscopic data obtained and by chemical evidence. They were defined as heptasaccharides or hexasaccharides, comprising d-fucose, d-glucose, and l-rhamnose units. Compounds 1, 3, and 5 were assigned the 11 S-hydroxyheptadecanoic acid as the aglycone, while compounds 2, 4, and 6 were found to possess 11 S-hydroxyhexadecanoic acid as the aglycone. Compounds 1, 3, and 5 are the first representatives of resin glycosides with 11 S-hydroxyheptadecanoic acid as the aglycone.


Assuntos
Convolvulus/química , Glicosídeos/química , Resinas Vegetais/química , Glicosídeos/isolamento & purificação , Hidrólise , Espectroscopia de Ressonância Magnética , Estrutura Molecular
11.
Environ Entomol ; 48(3): 747-754, 2019 06 07.
Artigo em Inglês | MEDLINE | ID: mdl-30958875

RESUMO

Zebra chip disease (ZC) in potato (Solanum tuberosum L. [Polemoniales: Solanaceae]) can produce unmarketable tubers with striped necrotic patterns. ZC is associated with the bacterium "Candidatus Liberibacter solanacearum" (Lso), which is transmitted by the potato psyllid, Bactericera cockerelli (Sulc) (Hemiptera: Triozidae). Potato psyllids are associated with numerous noncrop host plants, especially from the Solanaceae and Convolvulaceae; however, the contribution and importance of these hosts to ZC epidemiology in potato is poorly understood. To clarify seasonal phenologies on two such hosts, we sampled potato psyllids from bittersweet nightshade, Solanum dulcamara L. (Polemoniales: Solanaceae), and field bindweed, Convolvulus arvensis L. (Polemoniales: Convolvulaceae), over 2013-2017 and 2014-2016, respectively. Adult psyllids were sampled using yellow sticky traps, vacuum samples, and beat sheets. Each psyllid was tested for the presence of Lso by polymerase chain reaction. Psyllids often were abundant on bittersweet nightshade during May to November, with low numbers observed over each winter. Vacuum samples often captured more psyllids than other methods. Lso incidence was low except during 2016 when vacuum samples showed 23% incidence. Potato psyllids regularly overwinter on bittersweet nightshade in Idaho; however, differences in psyllid populations and Lso incidence from those found on potato suggest that this host plant may only partly contribute to infestations in potato. Observations of psyllids on field bindweed suggest only transient visits to this plant around potato harvest, with no evidence of overwintering and no Lso detected. Further work is needed to clarify how potato psyllid use of other noncrop hosts is related to their abundance in Idaho potato fields.


Assuntos
Convolvulus , Hemípteros , Solanum tuberosum , Solanum , Animais , Idaho , Doenças das Plantas
12.
J Chem Neuroanat ; 95: 115-122, 2019 01.
Artigo em Inglês | MEDLINE | ID: mdl-29051039

RESUMO

Convolvulus pluricaulis (Shankhapushpi) has long been used as traditional herbal medicine in India as nerve tonic. We studied the neuroprotective effects of C. pluricaulis extract (aqueous) against human microtubule-associated protein tau (hMAPτ) induced neurotoxicity in Alzheimer's disease (AD) Drosophila model. We analysed the lifespan, locomotor activity, τ protein level, reactive oxygen species (ROS), lipid peroxidation (LPO), catalase (CAT), superoxide dismutase (SOD) and acetylcholinesterase (AChE) activities in 10th, 20th and 30th days old control (wild type), τ control tauopathy Drosophila reared on C. pluricaulis supplemented with regular food or regular standard food. C. pluricaulis significantly offsets hMAPτ induced early death and extends the lifespan and diminishes the level of τ protein in tauopathy Drosophila. C. pluricaulis also enhances the antioxidant enzyme activities and ameliorates the τ-induced oxidative stress and restore the depleted AChE activity in the fly model. This study provides the first evidence that supplementation of C. pluricaulis along with the regular standard food ameliorate the neurotoxic effect of hMAPτ in AD Drosophila model and also reveals that it is a potent neuroprotective agent.


Assuntos
Doença de Alzheimer/patologia , Materia Medica/farmacologia , Fármacos Neuroprotetores/farmacologia , Extratos Vegetais/farmacologia , Animais , Convolvulus , Modelos Animais de Doenças , Drosophila melanogaster , Humanos , Estresse Oxidativo/efeitos dos fármacos , Tauopatias/patologia , Proteínas tau/genética , Proteínas tau/toxicidade
13.
Biomed Pharmacother ; 109: 1698-1708, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30551424

RESUMO

Depression is a heterogeneous disorder and has been regarded as an inflammatory disease. The aerial parts of the Convolvulus pluricaulis are used in Indian traditional medicines for the management of nervous disorders. However, the influence of methanolic extract of aerial parts of Convolvulus pluricaulis (CPE) on a chronic animal model of depression has not been investigated yet, and associated biochemical changes are still unclear. Therefore, this study investigates the effects of CPE on a chronic rat model of depression and explores its underlying mechanism of action on neuroinflammation and brain monoamines. The antidepressant-like effect of CPE (25, 50, and 100 mg/kg, p.o.) was depicted using the sucrose preference test and the forced swimming test (FST) while CUMS-induced alteration in the locomotor index was measured using the open field test (OFT) and actophotometer. A consecutive one-week treatment of CPE (50, and 100 mg/kg) or fluoxetine (10 mg/kg, p.o.) treatment significantly increased sucrose preference index, reduced immobility time in the FST, and increased the number of squares crossed, the number of rearing in the OFT and locomotion in the actophotometer in the CUMS-exposed rats. Moreover, elevated levels of pro-inflammatory cytokines IL-1ß, IL-6, TNF-α and liver biomarkers ALT, AST were also significantly reversed by CPE (50, and 100 mg/kg) or fluoxetine administration in the CUMS-exposed rats. Furthermore, a one-week treatment of CPE (50 and 100 mg/kg) or fluoxetine also remarkably restored the serotonin and noradrenaline levels in the hippocampus as well as in the prefrontal cortex of the CUMS-exposed rats. However, CPE (25 mg/kg) exerted insignificant protection against CUMS-induced depressive-like behavior and associated neuroinflammation. Therefore, this study demonstrates that CPE exerted antidepressant-like effect which could be mediated by anti-inflammatory potential, restoring liver biomarkers or monoaminergic responses in the stressed rats.


Assuntos
Anti-Inflamatórios/uso terapêutico , Antidepressivos/uso terapêutico , Convolvulus , Depressão/tratamento farmacológico , Fármacos Neuroprotetores/uso terapêutico , Estresse Psicológico/tratamento farmacológico , Animais , Anti-Inflamatórios/isolamento & purificação , Anti-Inflamatórios/farmacologia , Antidepressivos/isolamento & purificação , Antidepressivos/farmacologia , Doença Crônica , Depressão/metabolismo , Depressão/psicologia , Relação Dose-Resposta a Droga , Mediadores da Inflamação/antagonistas & inibidores , Mediadores da Inflamação/metabolismo , Masculino , Fármacos Neuroprotetores/isolamento & purificação , Fármacos Neuroprotetores/farmacologia , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/farmacologia , Extratos Vegetais/uso terapêutico , Ratos , Ratos Wistar , Estresse Psicológico/metabolismo , Estresse Psicológico/psicologia
14.
Fitoterapia ; 131: 209-214, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30385401

RESUMO

In this study, a resin glycoside fraction with cytotoxic activity was isolated from the alcoholic extract of C. arvensis whole plants. To describe the chemical feature of the resin glycosides, the fraction was alkaline hydrolyzed and four novel glycosidic acids, named arvensic acids A-D (1-4), were isolated. Their structures were thoroughly elucidated on the basis of spectroscopic data and chemical evidences. They all possess a same heptasacharride core, consisting of one D-fucose, two L-rhamnose and four d-glucose units. The difference among these glycosidic acids was placed on the aglycone, which is 12S-hydroxypentadecanoic acid for 1, 12S-hydroxyhexadecanoic acid for 2, 3S,12S-dihydroxypentadecanoic acid for 3, and 3S,12S- dihydroxyhexadecanoic acid for 4. These aglycones are rarely found in the structures of resin glycosides and are firstly identified in the genus Convolvulus.


Assuntos
Ácidos/química , Convolvulus/química , Glicosídeos/química , Resinas Vegetais/química , Células A549 , Ácidos/isolamento & purificação , China , Glicosídeos/isolamento & purificação , Células HeLa , Humanos , Hidrólise , Células MCF-7 , Compostos Fitoquímicos/isolamento & purificação
15.
J Pharm Pharmacol ; 70(11): 1572-1580, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30182365

RESUMO

OBJECTIVE: The aim of the study was to evaluate the effect of Convolvulus pluricaulis (CP; C. pluricaulis) methanolic extract on Triton WR-1339-induced hyperlipidaemia in rats. METHODS: The study comprised of six groups namely normal control, experimental control and treatment groups (100, 200 and 400 mg/kg of C. pluricaulis, and 65 mg/kg of Fenofibrate). Hyperlipidaemia was induced by a single intraperitoneal injection of Triton WR-1339 400 mg/kg in rats. Parameters such as lipid profile, oxidative stress, histological analysis and atherogenic index were evaluated. The plant extract was further studied by HPLC and LCMS, for analyses of active phytochemicals. KEY FINDINGS: The result of the study showed that C. pluricaulis significantly decreased total cholesterol, triglycerides, LDL-c, MDA levels and atherogenic index while the levels of HDL-c and GSH were found to be raised. Plant extract at the dose of 400 mg had a consistent effect on all lipid profile parameters. Lower doses (100 and 200 mg) did not produce a statistically significant reduction in LDL-c. In addition, the protective effect of C. pluricaulis was confirmed by histological analysis. Further, the findings of the study were found to be comparable with fenofibrate. CONCLUSIONS: Therefore, the present study suggests that C. pluricaulis has the potential for the treatment of hyperlipidaemia.


Assuntos
Convolvulus , Hiperlipidemias/tratamento farmacológico , Hipolipemiantes/farmacologia , Lipídeos/sangue , Extratos Vegetais/farmacologia , Polietilenoglicóis , Animais , Aterosclerose/sangue , Aterosclerose/induzido quimicamente , Aterosclerose/patologia , Aterosclerose/prevenção & controle , Biomarcadores/sangue , Convolvulus/química , Modelos Animais de Doenças , Feminino , Fenofibrato/farmacologia , Hiperlipidemias/sangue , Hiperlipidemias/induzido quimicamente , Hiperlipidemias/patologia , Hipolipemiantes/envenenamento , Masculino , Estresse Oxidativo/efeitos dos fármacos , Extratos Vegetais/isolamento & purificação , Placa Aterosclerótica , Ratos Wistar
16.
Sci Total Environ ; 633: 1272-1279, 2018 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-29758880

RESUMO

Green roofs provide important ecosystem services in urban areas. In Mediterranean and other semi-arid climate regions, most perennial plants on green roofs need to be irrigated during the dry season. However, the use of freshwater in such regions is scarce. Therefore, the possibility of using grey water should be examined. Coal ash, produced primarily from the burning of coal in power plants, constitutes an environmental contaminant that should be disposed. One option is to use ash as a growing substrate for plants. Here, we compare the effects of irrigating with grey- versus tap-water and using ash versus perlite as growing substrates in green roofs. The study was conducted in northern Israel in a Mediterranean climate. The design was full factorial with three factors: water-type (grey or tap-water)×substrate-type (coal ash vs perlite)×plant species (Phyla nodiflora, Convolvulus mauritanicus or no-plant). The development of plants and the quality of drainage water along the season, as well as quality of the used substrates were monitored. Both plant species developed well under all the experimental conditions with no effect of water type or substrate type. Under all treatments, both plant species enhanced electrical conductivity (EC) and chemical oxygen demand (COD) of the drainage water. In the summer, EC and COD reached levels that are unacceptable in water and are intended to be reused for irrigation. We conclude that irrigating with grey water and using coal ash as a growth substrate can both be implemented in green roofs. The drainage from tap water as well as from grey water can be further used for irrigating the roof, but for that, COD and EC levels must be lowered by adding a sufficient amount of tap water before reusing.


Assuntos
Óxido de Alumínio/química , Cinza de Carvão/química , Conservação dos Recursos Naturais/métodos , Convolvulus/crescimento & desenvolvimento , Água Potável/química , Lamiales/crescimento & desenvolvimento , Dióxido de Silício/química , Águas Residuárias/química
17.
J Photochem Photobiol B ; 181: 44-52, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29499463

RESUMO

Herein, we reported a facile, green and environmental friendlier biosynthesis of silver nanoparticles using the Convolvulus arvensis extract. The influences of various physicochemical factors such as the concentration of the plant extract, reaction time, and different pH levels were investigated by UV-Vis spectroscopy. The UV-Visible absorption spectrum of biogenic silver nanoparticles at λmax around ~400 nm suggested the biosynthesis of silver nanoparticles. Fourier transform infrared spectroscopy was employed to confirm the chemical transformation and role of various phyto-reductants in the conversion of Ag+ to Ag0. The surface morphology, topography, and elemental composition were analyzed by scanning electron microscopy, transmission electron microscopy, and energy dispersive X-ray spectroscopy, respectively. X-ray diffraction corroborated the face-centered cubic crystalline structure. The dynamic light scattering and zeta potential demonstrate the size distribution (90.9 nm) and surface charge (-18.5). Finally, the newly developed C. arvensis based silver nanoparticles were exploited as a catalyst for the catalytic reduction of azo dyes in the presence of NaBH4 as a reducing agent, and reducing the activity of C. arvensis based silver nanoparticles was evaluated by a decrease in optical density using UV-Vis spectrophotometer. The nanoparticles developed herein displayed potential efficiency for the degradation of all the tested dye pollutants. Conclusively, plant-based synthesis of nanoparticles provides an environmentally-responsive option for the reduction of highly environmental-polluted organic compounds including toxic azo dyes as compared to chemical and physical methods.


Assuntos
Convolvulus/química , Poluentes Ambientais/química , Nanopartículas Metálicas/química , Extratos Vegetais/química , Prata/química , Compostos Azo/química , Catálise , Convolvulus/metabolismo , Recuperação e Remediação Ambiental , Química Verde , Concentração de Íons de Hidrogênio , Microscopia Eletrônica de Transmissão , Oxirredução , Tamanho da Partícula , Espectrometria por Raios X , Espectrofotometria , Espectroscopia de Infravermelho com Transformada de Fourier , Difração de Raios X
18.
Int J Syst Evol Microbiol ; 67(9): 3590-3596, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-28866996

RESUMO

A bacterial strain designated Tese-5T was isolated from a water convolvulus field in Taiwan and characterized using the polyphasic taxonomic approach. Strain Tese-5T was an aerobic, Gram-stain-negative, non-motile, rod-shaped bacterium and formed bright yellow coloured colonies. Strain Tese-5T grew at 15-35 °C (optimum, 30 °C), with 0-1.0 % NaCl (optimum, 0-0.5 %) and at pH 5.5-7 (optimum, pH 6). The major fatty acids (>10 %) of strain Tese-5T were C18 : 1ω7c, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0. The polar lipid profile comprised phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine and sphingoglycolipid. The major polyamine was spermidine. The major isoprenoid quinone was Q-10. The DNA G+C content was 65.7 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Tese-5T belonged to the genus Novosphingobium and showed the highest levels of sequence similarity to Novosphingobium chloroacetimidivorans BUT-14T and Novosphingobium mathurense SM117T (96.3 %). Phenotypic characteristics of the novel strain also differed from those of the closest-related species of the genus Novosphingobium. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain Tese-5T represents a novel species in the genus Novosphingobium, for which the name Novosphingobium ipomoeaesp. nov. is proposed. The type strain is Tese-5T (=BCRC 80904T=LMG 28838T=KCTC 42656T).


Assuntos
Convolvulus/microbiologia , Filogenia , Sphingomonadaceae/classificação , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Hibridização de Ácido Nucleico , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Espermidina/química , Sphingomonadaceae/genética , Sphingomonadaceae/isolamento & purificação , Taiwan , Ubiquinona/química
19.
J Appl Microbiol ; 122(5): 1275-1285, 2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-28160348

RESUMO

AIMS: To identify a potential pathogenic isolate of fungus on Convolvulus arvensis and to determine its phytotoxic activity, which revealed the presence of toxic metabolites responsible for the toxicity against the target weed. METHODS AND RESULTS: A high virulent isolate of the fungus, Phoma crystallifera was isolated from symptomatic field bindweed in the west of Iran and was screened for the production of phytotoxins, which promoted necrosis on the detached leaves and seedlings of field bindweed in the bioassays. The isolate was distinct from other isolates of the fungi on the basis of morphological characteristics and the combined sequence database of the ITS region, partial LSU rDNA and ß-tubulin gene. Isolate P. crystalifera P6 produced the highest amount of phytotoxins after 21 days in a shacked culture of Richard's broth. The active metabolites were isolated from a cell-free culture filtrate by ethyl-acetate and purified by thin layer chromatography. The result indicated that six out of nine spots had phytotoxic activity in the bioassays, with Rf values of 0·16, 0·30, 0·36, 0·43, 0·57 and 0·81. CONCLUSIONS: Phoma crystallifera P6 and its active metabolites showed significant phytotoxic effects on the detached leaves of C. arvensis. SIGNIFICANCE AND IMPACT OF THE STUDY: To date, there are no reports of possible biocontrol agent(s) on C. arvensis in Iran. Thus, P. crystallifera P6 is introduced here as a severe pathogenic fungus and which can be used as a biocontrol agent against C. arvensis.


Assuntos
Ascomicetos/isolamento & purificação , Ascomicetos/metabolismo , Convolvulus/microbiologia , Micotoxinas/metabolismo , Doenças das Plantas/microbiologia , Ascomicetos/genética , Ascomicetos/patogenicidade , Convolvulus/efeitos dos fármacos , Irã (Geográfico) , Micotoxinas/farmacologia , Folhas de Planta/microbiologia , Plantas Daninhas/efeitos dos fármacos , Plantas Daninhas/microbiologia , Virulência , Controle de Plantas Daninhas
20.
J Photochem Photobiol B ; 167: 36-44, 2017 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-28039788

RESUMO

In this study, a rapid green synthesis of the silver nanoparticles (AgNPs) using leaves extract of Convolvulus arvensis was investigated. UV-Visible spectra showed the SPR band at around 430nm which proved the AgNP formation. The reduction of silver ions to nanoparticles was completed within 150min. Particle size analysis verified the formation of AgNPs with average size of 28nm, while their PDI was 0.2. XRD patterns verified the crystalline nature of produced AgNPs. SEM images verified the presence of spherical nanoparticles with no evidence of aggregations. FTIR analysis verified the involvement of the phenolic compounds in AgNP formation. It was found that the biosynthesized AgNPs have the antibacterial activity against human pathogen E. coli. Also, nanoparticles exhibit biofilm degrading activity against both bacterial strains of S. aureus and P. aeruginosa. Furthermore, the synthesized AgNPs prevented the coagulation of blood samples. The accelerated reduction of methylene blue (MB) in the presence of AgNPs with rate constant of 0.108min-1, confirmed the catalytic potential of nanoparticles.


Assuntos
Antibacterianos/farmacologia , Biofilmes/efeitos dos fármacos , Convolvulus/química , Nanopartículas Metálicas/química , Extratos Vegetais/farmacologia , Prata/química , Catálise , Humanos , Testes de Sensibilidade Microbiana , Microscopia Eletrônica de Varredura , Extratos Vegetais/química , Pseudomonas aeruginosa/efeitos dos fármacos , Análise Espectral , Staphylococcus aureus/efeitos dos fármacos , Difração de Raios X
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...