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1.
Anticancer Res ; 42(5): 2665-2673, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35489774

RESUMO

BACKGROUND: The purpose of this study was to evaluate the association of specific threshold values for changes in metabolic metrics measured from 1H magnetic resonance spectroscopic imaging (MRSI) to survival of patients with high-grade glioma treated with multimodality therapy. PATIENTS AND METHODS: Forty-four patients with newly diagnosed high-grade glioma were prospectively enrolled. Serial MRI and MRSI scans provided measures of tumor choline, creatine, and N-acetylaspartate (NAA). Cox regression analyses adjusted for patient age, KPS, and delivery of concurrent chemotherapy were used to assess the association of changes in metabolic metrics with survival. RESULTS: Median follow-up time for patients at risk was 13.4 years. Overall survival (OS) was longer in patients with ≤20% increase (vs. >20%) in normalized choline (p=0.024) or choline/NAA (p=0.024) from baseline to week 4 of RT. During this period, progression-free survival (PFS) was longer in patients with ≤40% increase (vs. >40%) in normalized choline (p=0.013). Changes in normalized creatine, choline/creatine, and NAA/creatine from baseline to mid-RT were not associated with OS. From baseline to post-RT, changes in metabolic metrics were not associated with OS or PFS. CONCLUSION: Threshold values for serial changes in choline metrics on mid-RT MRSI associated with OS and PFS were identified. Metabolic metrics at post-RT did not predict for these survival endpoints. These findings suggest a potential clinical role for MRSI to provide an early assessment of treatment response and could enable risk-adapted therapy in clinical trial development and clinical practice.


Assuntos
Neoplasias Encefálicas , Glioma , Neoplasias Encefálicas/diagnóstico por imagem , Neoplasias Encefálicas/terapia , Colina/metabolismo , Creatina/metabolismo , Glioma/diagnóstico por imagem , Glioma/metabolismo , Glioma/terapia , Humanos , Imageamento por Ressonância Magnética/métodos , Espectroscopia de Ressonância Magnética/métodos
2.
Rev Assoc Med Bras (1992) ; 68(8): 1011-1016, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-36134829

RESUMO

OBJECTIVE: Heart attack is one of the most common causes of sudden death in adults. Therefore, early detection of heart attack and investigation of potential new biomarkers are of great importance. We investigated whether perilipin-5 is a potential biomarker by examining changes in perilipin-5 serum levels along with high-sensitivity cardiac troponin I during a heart attack. METHODS: The subjects were divided into two groups: (1) control group and (2) patients with heart attack, with 150 people in each group. High-sensitivity cardiac troponin I, perilipin-5, total oxidant status, malondialdehyde, reduced glutathione, and superoxide dismutase levels in serum samples were measured. In addition, perilipin-5 mRNA expressions and protein levels were analyzed. RESULTS: There was no overall statistical difference between the demographic characteristics of the groups. However, high-density lipoprotein, creatine kinase, Creatine kinase myocardial band, aspartate amino transferase, lactate dehydrogenase, and calcium levels were higher in the heart attack group compared to the control group. We found that the high-sensitivity cardiac troponin I and perilipin-5 levels increased in the patients with heart attack (p<0.0001) compared to control. Although there was an insignificant increase in malondialdehyde levels in the heart attack group (p>0.05), there was a 35.9% increase in total oxidant status levels and a 33.5 and 24.1% decrease in glutathione and superoxide dismutase levels, respectively (p<0.01), compared to control. Perilipin-5 mRNA and protein levels in heart attack patients increased by 48.2 and 23.6%, respectively, compared to the control group (p<0.01). CONCLUSION: Our results showed that perilipin-5 together with high-sensitivity cardiac troponin I could be a promising biomarker in heart attack.


Assuntos
Infarto do Miocárdio , Troponina I , Adulto , Ácido Aspártico/metabolismo , Biomarcadores , Cálcio/metabolismo , Creatina/metabolismo , Creatina Quinase/metabolismo , Glutationa , Humanos , L-Lactato Desidrogenase , Metabolismo dos Lipídeos , Lipoproteínas HDL , Malondialdeído , Oxidantes , Perilipina-5/metabolismo , RNA Mensageiro/metabolismo , Superóxido Dismutase , Troponina I/metabolismo
3.
Int J Mol Sci ; 23(18)2022 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-36142560

RESUMO

The aim of the study was to investigate the effects of short-term oral administration of inorganic nitrate (NaNO3; n = 8) or placebo (NaCl; n = 9) (each 0.1 mmol/kg body weight/d for 9 days) on plasma amino acids, creatinine, and oxidative stress in healthy young men. At baseline, the plasma concentrations of amino acids did not differ between the groups. At the end of the study, the plasma concentrations of homoarginine (hArg; by 24%, p = 0.0001), citrulline and ornithine (Cit/Orn; by 16%, p = 0.015), and glutamine/glutamate (Gln/Glu; by 6%, p = 0.0003) were higher in the NaNO3 group compared to the NaCl group. The plasma concentrations of sarcosine (Sarc; by 28%, p < 0.0001), tyrosine (by 14%, p = 0.0051), phenylalanine (by 8%, p = 0.0026), and tryptophan (by 8%, p = 0.0047) were lower in the NaNO3 group compared to the NaCl group. These results suggest that nitrate administration affects amino-acid metabolism. The arginine/glycine amidinotransferase (AGAT) catalyzes two reactions: (1) the formation of l-homoarginine (hArg) and l-ornithine (Orn) from l-arginine (Arg) and l-lysine (Lys): Arg + Lys <-> hArg + Orn, with equilibrium constant Kharg; (2) the formation of guanidinoacetate (GAA) and Orn from Arg and glycine (Gly): Arg + Gly <-> GAA + Orn, with equilibrium constant Kgaa. The plasma Kgaa/KhArg ratio was lower in the NaNO3 group compared to the NaCl group (1.57 vs. 2.02, p = 0.0034). Our study suggests that supplementation of inorganic nitrate increases the AGAT-catalyzed synthesis of hArg and decreases the N-methyltransferase-catalyzed synthesis of GAA, the precursor of creatine. To our knowledge, this is the first study to demonstrate elevation of hArg synthesis by inorganic nitrate supplementation. Remarkably, an increase of 24% corresponds to the synthesis capacity of one kidney in healthy humans. Differences in the association between plasma concentrations of amino acids in the NaNO3 and NaCl groups suggest changes in amino-acid homeostasis. Plasma concentrations of the oxidative stress marker malondialdehyde (MDA) did not change after supplementation of NaNO3 or NaCl over the whole exercise time range. Plasma nitrite concentration turned out to be a more discriminant marker of NaNO3 ingestion than plasma nitrate (area under the receiver operating characteristic curve: 0.951 vs. 0.866, p < 0.0001 each).


Assuntos
Homoarginina , Nitratos , Arginina/metabolismo , Citrulina , Creatina , Creatinina , Suplementos Nutricionais , Glutamatos , Glutamina , Glicina , Homoarginina/metabolismo , Humanos , Lisina , Masculino , Malondialdeído , Metiltransferases , Nitritos , Ornitina , Fenilalanina , Sarcosina , Cloreto de Sódio , Triptofano , Tirosina
4.
PLoS One ; 17(9): e0274883, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36155549

RESUMO

BACKGROUND: Creatinine is widely used to estimate renal function, but this is not practical in critical illness. Low creatinine has been associated with mortality in many clinical settings. However, the associations between predialysis creatinine level, Sepsis-related Organ Failure Assessment (SOFA) score, fluid overload, and mortality in acute kidney injury patients receiving dialysis therapy (AKI-D) has not been fully addressed. METHODS: We extracted data for AKI-D patients in the eICU and MIMIC databases. We conducted a retrospective observational cohort study using the eICU dataset. The study cohort was divided into the high-creatine group and the low-creatinine group by the median value (4 mg/dL). The baseline patient information included demographic data, laboratory tests, medications, and comorbid conditions. The independent association of creatinine level with 30-day mortality was examined using multivariate logistic regression analysis. In sensitivity analyses, the associations between creatinine, SOFA score, and mortality were analyzed in patients with or without fluid overload. We also carried out an external validity using the MIMIC dataset. RESULTS: In all 1,600 eICU participants, the 30-day mortality rate was 34.2%. The crude overall mortality rate in the low-creatinine group (44.9%) was significantly higher than that in the high-creatinine group (21.9%; P < 0.001). In the fully adjusted models, the low-creatinine group was associated with a higher risk of 30-day mortality (odds ratio, 1.77; 95% confidence interval, 1.29-2.42; P < 0.001) compared with the high-creatinine group. The low-creatinine group had higher SOFA and nonrenal SOFA scores. In sensitivity analyses, the low-creatinine group had a higher 30-day mortality rate with regard to the BMI or albumin level. Fluid overloaded patients were associated with a significantly worse survival in the low-creatinine group. The results were consistent when assessing the external validity using the MIMIC dataset. CONCLUSIONS: In patients with AKI-D, lower predialysis creatinine was associated with increased mortality risk. Moreover, the mortality rate was substantially higher in patients with lower predialysis creatinine with concomitant elevation of fluid overload status.


Assuntos
Injúria Renal Aguda , Sepse , Injúria Renal Aguda/etiologia , Albuminas , Creatina , Creatinina , Humanos , Unidades de Terapia Intensiva , Prognóstico , Diálise Renal , Estudos Retrospectivos , Fatores de Risco , Sepse/complicações
5.
Nutrients ; 14(18)2022 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-36145081

RESUMO

Methionine restriction and selenium supplementation are recommended because of their health benefits. As a major nutrient form in selenium supplementation, selenomethionine shares a similar biological process to its analog methionine. However, the outcome of selenomethionine supplementation under different methionine statuses and the interplay between these two nutrients remain unclear. Therefore, this study explored the metabolic effects and selenium utilization in HepG2 cells supplemented with selenomethionine under deprived, adequate, and abundant methionine supply conditions by using nuclear magnetic resonance-based metabolomic and molecular biological approaches. Results revealed that selenomethionine promoted the proliferation of HepG2 cells, the transcription of selenoproteins, and the production of most amino acids while decreasing the levels of creatine, aspartate, and nucleoside diphosphate sugar regardless of methionine supply. Selenomethionine substantially disturbed the tricarboxylic acid cycle and choline metabolism in cells under a methionine shortage. With increasing methionine supply, the metabolic disturbance was alleviated, except for changes in lactate, glycine, citrate, and hypoxanthine. The markable selenium accumulation and choline decrease in the cells under methionine shortage imply the potential risk of selenomethionine supplementation. This work revealed the biological effects of selenomethionine under different methionine supply conditions. This study may serve as a guide for controlling methionine and selenomethionine levels in dietary intake.


Assuntos
Selênio , Selenometionina , Aminoácidos , Ácido Aspártico , Colina , Citratos , Creatina , Suplementos Nutricionais , Glicina , Células Hep G2 , Humanos , Hipoxantinas , Lactatos , Metionina/metabolismo , Metionina/farmacologia , Açúcares de Nucleosídeo Difosfato , Racemetionina , Selênio/metabolismo , Selênio/farmacologia , Selenometionina/farmacologia , Selenoproteínas
6.
Nutrients ; 14(17)2022 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-36079854

RESUMO

The reactive 1,2-dicarbonyl compound methylglyoxal (MGO) is consumed with food and its concentrations decrease during digestion. In the present paper, the reaction of MGO with creatine, arginine, and lysine during simulated digestion, and its reaction with creatine during the digestion in human volunteers, was studied. Therefore, simulated digestion experiments with a gastric and an intestinal phase were performed. Additionally, an intervention study with 12 subjects consuming MGO-containing Manuka honey and creatine simultaneously or separately was conducted. Derivatization with o-phenylenediamine and HPLC-UV was used to measure MGO, while creatine and glycated amino compounds were analyzed via HPLC-MS/MS. We show that MGO quickly reacts with creatine and arginine, but not lysine, during simulated digestion. Creatine reacts with 56% of MGO to form the hydroimidazolone MG-HCr, and arginine reacted with 4% of MGO to form the hydroimidazolone MG-H1. In the intervention study, urinary MG-HCr excretion is higher in subjects who consumed MGO and creatine simultaneously compared to subjects who ingested the substances separately. This demonstrates that the 1,2-dicarbonyl compound MGO reacts with amino compounds during human digestion, and glycated adducts are formed. These contribute to dietary glycation products consumed, and should be considered in studies investigating their physiological consequences.


Assuntos
Creatina , Aldeído Pirúvico , Arginina , Digestão , Produtos Finais de Glicação Avançada , Voluntários Saudáveis , Humanos , Lisina , Óxido de Magnésio , Espectrometria de Massas em Tandem
7.
Int J Mol Sci ; 23(18)2022 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-36142537

RESUMO

Tuberous sclerosis complex (TSC) is caused by mutations in the hamartin (TSC1) or tuberin (TSC2) genes. Using a mouse model of TSC renal cystogenesis that we have previously described, the current studies delineate the metabolic changes in the kidney and their relation to alterations in renal gene expression. To accomplish this, we compared the metabolome and transcriptome of kidneys from 28-day-old wildtype (Wt) and principal cell-specific Tsc1 KO (Tsc1 KO) mice using targeted 1H nuclear magnetic resonance targeted metabolomic and RNA-seq analyses. The significant changes in the kidney metabolome of Tsc1 KO mice included reductions in the level of several amino acids and significant decreases in creatine, NADH, inosine, UDP-galactose, GTP and myo-inositol levels. These derangements may affect energy production and storage, signal transduction and synthetic pathways. The pertinent derangement in the transcriptome of Tsc1 KO mice was associated with increased collecting duct acid secretion, active cell division and the up-regulation of signaling pathways (e.g., MAPK and AKT/PI3K) that suppress the TSC2 GTPase-activating function. The combined renal metabolome and transcriptome alterations observed in these studies correlate with the unregulated growth and predominance of genotypically normal A-intercalated cells in the epithelium of renal cysts in Tsc1 KO mice.


Assuntos
Esclerose Tuberosa , Proteínas Supressoras de Tumor , Carotenoides , Creatina/metabolismo , GTP Fosfo-Hidrolases/genética , Galactose/metabolismo , Guanosina Trifosfato/metabolismo , Humanos , Inosina/metabolismo , Inositol/metabolismo , Rim/metabolismo , Metaboloma , NAD/metabolismo , Fosfatidilinositol 3-Quinases/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Transcriptoma , Esclerose Tuberosa/metabolismo , Proteína 1 do Complexo Esclerose Tuberosa/genética , Proteína 1 do Complexo Esclerose Tuberosa/metabolismo , Proteína 2 do Complexo Esclerose Tuberosa/genética , Proteína 2 do Complexo Esclerose Tuberosa/metabolismo , Proteínas Supressoras de Tumor/genética , Difosfato de Uridina/metabolismo , Vitamina A/análogos & derivados
8.
J Pak Med Assoc ; 72(7): 1369-1372, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-36156562

RESUMO

OBJECTIVE: To find out the association between poor glycaemic levels and biochemical and haematological abnormalities in patients of corona virus disease-2019. METHODS: The prospective, observational, cohort study was conducted at the Combined Military Hospital, Quetta, Pakistan, from September 2020 to February 2021, and comprised all patients who tested positive for coronavirus disease-2019 on polymerase chain reaction test and were subsequently admitted. The patients were divided into two groups on the basis of random blood glucose level at the time of admission; ≥11.1mmol/l (206mg/dl) in group A and 4-11.1mmol (74-206mg/dl) in group B. Association between categorical variables was evaluated and hazard ratio was measured. Data was analysed using SPSS 21. RESULTS: Of the 349 patients, there were 56(16%) in group A; 40(71.4%) males and 16(28.6%) females with age range 39-61 years. There were 293(84%) subjects in group B; 239(81.5%) males and 54(18.5%) females with age range 27-53 years. Overall, 75(21.4%) patients were known type 2 diabetics. A significant association was found between poor glycaemic control and raised levels of C-reactive protein, lactate dehydrogenase, ferritin, erythrocyte sedimentation rate, troponin, creatine kinase, creatine kinase-MB, alanine aminotransferase, creatinine and D-dimers (p<0.05). Inter-group differences were significant for acute kidney injury, acute liver injury, Intensive care unit admission for coagulation abnormalities and for overall mortality (p<0.05). CONCLUSIONS: Poor glycaemic control was found to be a risk factor for developing multisystem complications in patients of coronavirus disease-2019.


Assuntos
COVID-19 , Adulto , Alanina Transaminase , Glicemia/análise , Proteína C-Reativa/análise , COVID-19/epidemiologia , Estudos de Coortes , Creatina , Creatina Quinase Forma MB , Creatinina , Feminino , Ferritinas , Humanos , L-Lactato Desidrogenase , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , SARS-CoV-2 , Centros de Atenção Terciária , Troponina
9.
Turk J Med Sci ; 52(1): 11-20, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36161599

RESUMO

BACKGROUND: COVID - 19 disease may be seen with different clinical presentations in pregnant women. Comorbid diseases are important factors affecting the progression of this disease. In this study, we aimed to evaluate the clinical and laboratory findings in pregnant women with COVID - 19 who had no comorbid disease. METHODS: This retrospective designed study included 217 patients with Covid PCR positive in typically COVID - 19 clinic. The patients were classified into asymptomatic, nonsevere, and severe disease groups. The symptoms, laboratory results, hospital followups and intensive care records of the patients and the findings of new borns are presented. RESULTS: Most of the patients (78%) were in the third trimester of pregnancy, and 103 patients in the study group had severe disease. Fever in the non-severe group and respiratory distress in the severe group were the most common symptoms in the patients. The severe clinical manifestations were specifically observed in the third trimester patients. In the severe group, neutrophil, lactat dehydrogenase, ferritin, CK - MB, IL - 6, and hospital stay were statistically higher than those in other groups (p < 0.05). Increase in BUN and creatine were the most predictive parameters in intensive care admission. While the intensive care unit (ICU) requirement was higher in patients in the severe group, premature birth was observed more frequently in the severe group (p < 0.05) .


Assuntos
COVID-19 , COVID-19/epidemiologia , Comorbidade , Creatina , Feminino , Ferritinas , Humanos , Oxirredutases , Gravidez , Estudos Retrospectivos , SARS-CoV-2
10.
Methods Mol Biol ; 2546: 129-140, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36127584

RESUMO

Cerebral creatine deficiency syndromes are caused by the dysfunctional creatine biosynthesis or transport and comprise three hereditary neurodevelopmental defects including arginine-glycine amidinotransferase (AGAT), guanidinoacetate methyltransferase (GAMT), and creatine transporter deficiencies. All conditions are characterized by seizures, intellectual disability, and behavioral abnormalities. Laboratory diagnosis of these disorders relies on the determination of creatine and guanidinoacetate concentrations in both plasma and urine. Here we describe a rapid quantitative UPLC/MS/MS method for the simultaneous determination of these analytes using a normal-phase HILIC column after analyte derivatization. The approach is suitable for neonatal screening follow-ups and monitoring of the treatment for creatine deficiency syndromes.


Assuntos
Creatina , Guanidinoacetato N-Metiltransferase , Glicina/análogos & derivados , Humanos , Recém-Nascido , Triagem Neonatal , Síndrome , Espectrometria de Massas em Tandem
11.
Mediators Inflamm ; 2022: 1623478, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36105682

RESUMO

Background: Middle-aged (45-59 years old) patients with major depressive disorder (MDD) have a predilection for dementia and cognitive disorders (CDs); however, the characteristics and mechanisms of CDs in these patients remain unclear. There are also known connections between thyroid-stimulating hormone (TSH), brain biochemical metabolism, and cognitive function (CF); however, there is scanty of information about these connections in middle-aged MDD patients. Methods: Cognitive assessment was performed on 30 first-episode, untreated middle-aged patients with MDD and 30 well-matched healthy controls (HCs) using the MATRICS Consensus Cognitive Battery (MCCB). N-acetyl aspartate (NAA)/creatine (Cr) and choline (Cho)/Cr ratios in the prefrontal cortex (PFC) and cerebellum were also obtained via proton magnetic resonance spectroscopy (1H-MRS), and the TSH level was measured by chemiluminescence analysis. Results: MDD patients presented significantly lower processing speed, working memory, verbal learning, reasoning problem-solving, visual learning, and composite cognition scores than controls, with a statistically lower NAA/Cr ratio in the right cerebellum. Age was positively related to reasoning problem-solving in the MDD group (r = 0.6249, p = 0.0220). Education also showed a positive association with visual learning, social cognition, and composite cognition. The 24-item Hamilton Depression Rating Scale (HDRS-24) score was negatively related to all domains of CF. TSH levels were markedly decreased in the MDD group, and a positive connection was determined between the NAA/Cr ratio in the right PFC and the TSH level. Conclusions: Middle-aged MDD patients have multidimensional CDs. There are changes in PFC and cerebellar biochemical metabolism in middle-aged patients with MDD, which may be related to CDs or altered TSH levels.


Assuntos
Transtorno Depressivo Maior , Cognição , Creatina/metabolismo , Humanos , Pessoa de Meia-Idade , Espectroscopia de Prótons por Ressonância Magnética/métodos , Tireotropina
12.
Zhong Nan Da Xue Xue Bao Yi Xue Ban ; 47(7): 910-919, 2022 Jul 28.
Artigo em Inglês, Chinês | MEDLINE | ID: mdl-36039588

RESUMO

OBJECTIVES: Many neuropsychiatric diseases are related to the abnormal development of brain tissue in infants. This study aims to analyze the changes in the parameters of magnetic resonance spectroscopy (MRS) and diffusion tensor imaging (DTI) in brain development of infants aged from 2 months to 2 years old, and to explore the value of MRS combined with DTI in evaluating brain development of infants aged from 2 months to 2 years old. METHODS: A total of 116 normal infants, who received whole brain MRS and DTI examinations after delivery in Children Hospital of Shanxi Province from September 2020 to May 2021, were selected and were divided into a group A (n=7, at the age of 2-6 months), a group B (n=28, at the age of 7-12 months), a group C (n=41, at the age of 13-18 months), and a group D (n=40, at the age of 19-24 months). After collecting the MRS and DTI data, statistical analysis was performed to compare DTI parameters and MRS metabolic products ratio. RESULTS: There were significant differences in the DTI parameters of frontal lobe, temporal lobe, occipital lobe, hind limb of internal capsule, fore limb of internal capsule, knee of corpus callosum, splenium of corpus callosum, and optic radiation among the 4 groups (P<0.05 or P<0.01). The values of fractional anisotropy (FA) showed an upward trend from the group A to the group D, while the values of apparent diffusion coefficient (ADC), axial diffusivity (AD), and radial diffusivity (RD) showed a downward trend, and the changes of parameters tended to slow down with age. In the left or right lentiform nucleus, the ratio of choline (Cho)/creatine (Cr) was decreased from the group A to the group D, and the group D was significantly lower than the group A and B (all P<0.01). The ratio of Cho/N-acetyl aspartate (NAA) was decreased from the group A to the group D, and the group D was significantly lower than the group A, B, and C (left lentiform nucleus, P<0.05 or P<0.01) or the group A, B (right lentiform nucleus, both P<0.01). The ratio of glutamine/glutamate (Glx)/Cr was decreased from the group A to the group D, and the group D was significantly lower than the group A, B and C (P<0.05 or P<0.01). The ratio of myo-inositol (mI)/Cr was increased from the group A to the group D, and the group D was significantly higher than the group A, B, and C (P<0.05 or P<0.01). The ratio of NAA/Cr was increased from the group A to the group D, and the group B, C, and D were significantly higher than the group A (P<0.05 or P<0.01). The ratios of mI/Cr and NAA/Cr in different brain regions from the group A to the group D showed an upward trend, and the ratios of Cho/Cr, Cho/NAA, and Glx/Cr showed a downward trend. The variation of each parameter tends to decrease with age. CONCLUSIONS: MRS and DTI can detect the brain development of infants aged from 2 months to 2 years old, and provide a basis for predicting brain diseases.


Assuntos
Imagem de Tensor de Difusão , Imageamento por Ressonância Magnética , Ácido Aspártico , Encéfalo/patologia , Pré-Escolar , Colina , Creatina , Imagem de Difusão por Ressonância Magnética/métodos , Imagem de Tensor de Difusão/métodos , Humanos , Lactente , Espectroscopia de Ressonância Magnética/métodos
13.
Tunis Med ; 100(3): 262-269, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36005919

RESUMO

AIM: To evaluate the effects of dry cupping therapy (DCT) and creatine supplementation (CS) on cardiovascular and inflammatory responses to the Wingate test. METHODS: In this quasi-experimental study, 12 male handball young players were selected in a crossover design. Players were studied in four conditions: DCT; CS; CS+DCT, control. In all conditions, blood pressure, heart-rate, and body composition were measured pre- and post- Wingate test. Players were assessed by the Wingate test in two 30-second phases with a 1-minute break between the phases. Blood [lactate-dehydrogenase (LDH), creatine phosphokinase (CK)] was drawn pre- and immediately post- the Wingate test. In players with CS condition, 60 g of creatine was consumed per day in three consecutive days prior to the study (3 meals of 20 g in morning, noon, and night). The DCT was performed after Wingate test to consider its possible effects for alleviating the muscle injury markers. Data were evaluated using analysis of covariance followed by a post-hoc Bonferoni test. RESULTS: The heart-rate' means in DCT, CS and CS+DCT conditions were lower compared to the control-condition (p.


Assuntos
Ventosaterapia , Esportes , Composição Corporal , Creatina/farmacologia , Estudos Cross-Over , Suplementos Nutricionais , Humanos , Masculino
14.
Front Cell Infect Microbiol ; 12: 887407, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36034698

RESUMO

Background: Parkinson's disease (PD) is a common neurodegenerative disorder, accompanied by motor deficits as well as gastrointestinal dysfunctions. Recent studies have proved that the disturbance of gut microbiota and metabolism contributes to the pathogenesis of PD; however, the mechanisms underlying these effects have yet to be elucidated. Curcumin (CUR) has been reported to provide neuroprotective effects on neurological disorders and modulate the gut flora in intestinal-related diseases. Therefore, it is of significant interest to investigate whether CUR could exert a protective effect on PD and whether the effect of CUR is dependent on the intestinal flora and subsequent changes in metabolites. Methods: In this study, we investigated the neuroprotective effects of CUR on a mouse model of PD induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). 16S rRNA sequencing was performed to explore the profile of the gut microbiota among controls, MPTP-treated mice and CUR-treated mice. Then, antibiotic treatment (ABX) and fecal microbiota transplantation (FMT) experiments were conducted to examine the role of intestinal microbes on the protective effects of CUR in PD mice. Furthermore, ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS)-based metabolomics analysis was used to identify the landscape of the CUR-driven serum metabolome. Finally, Pearson's analysis was conducted to investigate correlations between the gut flora-metabolite axis and CUR-driven neuroprotection in PD. Results: Our results showed that CUR intervention effectively improved motor deficits, glial cell activation, and the aggregation of α-synuclein (α-syn) in MPTP-treated mice. 16S rRNA sequencing showed elevated abundances of Muribaculaceae, Lactobacillaceae, Lachnospiraceae and Eggerthellaceae but depleted abundances of Aerococcaceae and Staphylococcaceae in CUR-treated mice when compared with MPTP mice. ABX and FMT experiments further confirmed that the gut microbiota was required for CUR-induced protection in PD mice. Serum metabolomics analysis showed that CUR notably upregulated the levels of tyrosine, methionine, sarcosine and creatine. Importantly, strong correlations were identified among crucial taxa (Aerococcaceae, Staphylococcaceae, Muribaculaceae, Lactobacillaceae, Lachnospiraceae and Eggerthellaceae), pivotal metabolites (tyrosine, methionine, sarcosine and creatine) and the motor function and pathological results of mice. CUR treatment led to a rapid increase in the brain levels of tyrosine and levodopa (dopa) these changes were related to the abundances of Lactobacillaceae and Aerococcaceae. Conclusions: CUR exerts a protective effect on the progression of PD by modulating the gut microbiota-metabolite axis. Lactobacillaceae and Aerococcaceae, along with key metabolites such as tyrosine and dopa play a dominant role in CUR-associated neuroprotection in PD mice. Our findings offer unique insights into the pathogenesis and potential treatment of PD.


Assuntos
Curcumina , Microbioma Gastrointestinal , Fármacos Neuroprotetores , Doença de Parkinson , 1-Metil-4-Fenil-1,2,3,6-Tetra-Hidropiridina , Animais , Creatina , Modelos Animais de Doenças , Levodopa , Metaboloma , Metionina , Camundongos , Camundongos Endogâmicos C57BL , Doenças Neuroinflamatórias , RNA Ribossômico 16S , Sarcosina
15.
Neuroscience ; 501: 72-84, 2022 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-35961525

RESUMO

Swimming training (ST) can mitigate functional disorders in neurological diseases, but the effect and mechanism of ST in improving the neurological function of intracerebral haemorrhage (ICH) have not been reported. Our study aimed to explore the protective effect of early ST on ICH mice and its relationship with the serine-threonine kinase (Akt)/glycogen synthase kinase 3ß (GSK3ß) pathway. Our findings showed that the ICH model mice had poor behavioural manifestations in the Y maze test and open field test compared to the ST group and sham group. The modified neurological severity score was increased in the ICH mice, and 7 days of ST intervention significantly attenuated the neurological deficits. The ratios of myo-inositol/creatine, lactate/creatine and glutamate/creatine were decreased, and the ratios of N-acetylaspartate/creatine and choline/creatine were increased in the ICH mice with ST intervention. ST intervention decreased the expression of Iba1 and GFAP. Seven days of ST significantly increased the expression of p-Akt/Akt compared to that in the ICH mice. Furthermore, the Akt kinase inhibitor GSK690693 exacerbated neurological impairment, increased the expression of Iba1, GFAP and Bax/Bcl-2, and reversed the anti-apoptotic effects and anti-glia activation of ST, which was associated with the inhibition of p-Akt/Akt and p-GSK3ß/GSK3ß expression. These results indicated that the protective role of ST in ICH was mediated via the Akt/GSK3ß pathway. In conclusion, ST displayed neuroprotection by inhibiting apoptosis and glial activation in ICH mice by activating the Akt/GSK3ß signalling pathway.


Assuntos
Fármacos Neuroprotetores , Proteínas Serina-Treonina Quinases , Animais , Apoptose , Hemorragia Cerebral/metabolismo , Colina/farmacologia , Creatina/farmacologia , Glutamatos , Glicogênio Sintase Quinase 3 beta , Inositol/farmacologia , Lactatos/farmacologia , Camundongos , Fármacos Neuroprotetores/farmacologia , Proteínas Proto-Oncogênicas c-akt/metabolismo , Proteínas Proto-Oncogênicas c-bcl-2 , Natação , Proteína X Associada a bcl-2
16.
J Int Soc Sports Nutr ; 19(1): 529-542, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35966022

RESUMO

Background: Creatine monohydrate (CrM) supplementation has been shown to be an effective and safe nutritional supplement to improve performance; however, the impact of CrM supplementation in young basketball players is less clear. This study evaluated the effects of CrM supplementation during a strength and conditioning training (SCT) program on lower-limb strength parameters and performance in under-16 (U16) basketball players. Methods: Twenty-three male U16 basketball players participated in this study (14.3 ± 0.4 years; BMI: 20.7 ± 2.2 kg∙m-2). The players were randomly assigned to either a CrM group (n = 12) that ingested 0.1 g·kg-1·day-1 of CrM or to a non-supplemented control group (n = 11, CON). The athletes participated in an 8-week SCT program consisting of two lower-limb resistance-training sessions and two plyometric sessions per week. Squat jump (SJ), drop jump (DP), countermovement jump (CMJ), and Abalakov (ABK) jump power tests as well as basketball performance (points and minutes per game) were measured before, during and/or after the intervention. Data were analyzed using a general linear model with repeated measures with independent Student's t-test pairwise comparisons. Results: The results (95% confidence interval for mean change from baseline) show that there were significant differences for all variables for CrM and CON, respectively: SJ (cm): 2.6 - 6.4, P < 0.01 and 2.2-5.1 P < 0.01; DJ (cm): 2.5-5.6, P < 0.01, and 1.8-4.4, P < 0.01; CMJ (cm): 0.3-0.8, P < 0.01, and 0.2-0.5, P < 0.01; ABK (cm): 2.8-5.5, P < 0.01 and 0.7-2.6, P = 0.003. A significant group x time interaction (p = 0.003, ηp 2 = 0.342) was observed in ABK performance. No significant group x time effects were seen in squat jump (p = 0.449, ηp 2 = 0.028), drop jump (p = 0.143, ηp 2 = 0.099), or counter movement jump (p = 0.304, ηp 2 = 0.05). A significant interaction effect was also observed in points per game (p = 0.049, ηp 2 = 0.149), while a non-significant but medium effect was seen in minutes per game (p = 0.166, ηp 2 = 0.094). Conclusions: CrM supplementation in conjunction with resistance and plyometric training increased the lower-limb ABK power and scoring performance in U16 basketball players.


Assuntos
Desempenho Atlético , Basquetebol , Treinamento de Força , Creatina , Humanos , Masculino , Força Muscular , Treinamento de Força/métodos
17.
J Strength Cond Res ; 36(9): 2663-2670, 2022 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-36000773

RESUMO

ABSTRACT: Fazio, C, Elder, CL, and Harris, MM. Efficacy of alternative forms of creatine supplementation on improving performance and body composition in healthy subjects: a systematic review. J Strength Cond Res 36(9): 2663-2670, 2022-Novel forms of creatine have appeared in the marketplace with substantial claims of improved efficacy compared to creatine monohydrate (CrM). The purpose of this study was to conduct a systematic review on alternative forms of creatine to determine (a) whether they are effective ergogenic aids and (b) whether they outperform CrM. A separate comparison was conducted to determine average cost of various forms of creatine. Cochrane Central Register of Controlled Trials (CENTRAL), PubMed, Medline, and Google Scholar were systematically reviewed according to PRISMA guidelines. The design of the review was set to answer the PICOS model (subjects, interventions, comparators, outcomes, and study design). Seventeen randomized placebo controlled clinical trials examining exercise performance outcomes and body composition were included in the analysis. Magnesium-creatine chelate and creatine citrate, malate, ethyl ester, nitrate, and pyruvate were the only forms researched in the literature. Of these studies, only 3 studies compared the alternative creatine form to CrM, making it difficult to compare efficacy to CrM. There were no consistent findings of performance enhancement among alternative forms of creatine when compared to placebo. A review of the marketplace shows that CrM is the lowest cost form of creatine. Due to the paucity of studies on alternative forms of creatine as well as high prices on the market of these alternative forms, CrM remains as the most extensively studied form of creatine that shows efficacy, safety, and lowest cost to consumer.


Assuntos
Creatina , Substâncias para Melhoria do Desempenho , Idoso , Composição Corporal , Suplementos Nutricionais , Voluntários Saudáveis , Humanos , Substâncias para Melhoria do Desempenho/farmacologia
18.
Chemosphere ; 307(Pt 4): 135963, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36007736

RESUMO

Persistent organic pollutants (POPs) are lipophilic environmental toxins, and the level of chemicals accumulated in the body through the food chain has been linked to the incidence of diseases such as type 2 diabetes, cardiovascular disease, and cancer. We analyzed the concentration of POPs and circulating metabolites and investigated the associations between the concentration of plasma metabolites and the levels of polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCPs) to determine the effect of the accumulation of POPs in human samples. Metabolic profiling of plasma from 276 Korean participants was performed using 1H nuclear magnetic resonance (NMR) and statistical analyses. The concentrations of PCBs and OCPs in each sample were measured. Correlation analysis and a covariate-adjusted general linear model (GLM) were used to investigate the association of the concentration of POPs with circulating metabolites in human blood samples. We found that four categories of Σ6PCBs and Σ5OCPs based on rank were significantly correlated with 4 and 5 metabolites, respectively, after adjusting for confounding factors, including age, sex, body mass index (BMI), smoking status, alcohol intake, physical activity, triglycerides, and total cholesterol. According to the GLM analyses, 3 metabolites, namely, creatinine, acetate, and formate, among the 4 correlated metabolites were associated with four categories of rank-based Σ6PCBs. On the other hand, the quartiles of the rank-based Σ5OCPs were not associated with any circulating metabolites among the 5 correlated metabolites. Our findings indicate that the metabolites related to short-chain fatty acids and creatine can be useful risk indicators for estimating the effect of PCB exposure.


Assuntos
Diabetes Mellitus Tipo 2 , Poluentes Ambientais , Hidrocarbonetos Clorados , Praguicidas , Bifenilos Policlorados , Colesterol , Creatina , Creatinina , Formiatos , Humanos , Espectroscopia de Ressonância Magnética , Poluentes Orgânicos Persistentes , Triglicerídeos
19.
Rinsho Shinkeigaku ; 62(9): 716-721, 2022 Sep 28.
Artigo em Japonês | MEDLINE | ID: mdl-36031377

RESUMO

A 59-year-old woman presented with right hemiparesis and was transported from outside hospital. MRI revealed acute infarction and the left middle cerebral artery M2 occlusion. Intravenous infusion of recombinant tissue-type plasminogen activator, and mechanical thrombectomy (MT) were performed. The cause of cerebral infarction was diagnosed as Libman-Sacks endocarditis. She discharged without sequelae. After 10 months later, she presented with mild cognitive decline, and MRI showed new white matter lesion in left deep white matter. In magnetic resonance spectroscopy, the lesion showed an increased rate of choline/creatine, and a decreased rate of N-acetylaspartate/creatine, elevated lactate peak. When new higher brain dysfunction presented after recanalization by MT, it might be related to the delayed white matter lesion.


Assuntos
Endocardite , Substância Branca , Colina , Creatina , Endocardite/complicações , Feminino , Humanos , Infarto da Artéria Cerebral Média/diagnóstico por imagem , Infarto da Artéria Cerebral Média/etiologia , Infarto da Artéria Cerebral Média/terapia , Lactatos , Pessoa de Meia-Idade , Trombectomia/métodos , Ativador de Plasminogênio Tecidual , Substância Branca/diagnóstico por imagem
20.
Biosensors (Basel) ; 12(7)2022 Jul 07.
Artigo em Inglês | MEDLINE | ID: mdl-35884299

RESUMO

A novel assay platform consisting of a microfluidic sliding double-track paper-based chip and a hand-held Raspberry Pi detection system is proposed for determining the albumin-to-creatine ratio (ACR) in human urine. It is a clinically important parameter and can be used for the early detection of related diseases, such as renal insufficiency. In the proposed method, the sliding layer of the microchip is applied and the sample diffuses through two parallel filtration channels to the reaction/detection areas of the microchip to complete the detection reaction, which is a simple method well suited for self-diagnosis of ACR index in human urine. The RGB (red, green, and blue) value intensity signals of the reaction complexes in these two reaction zones are analyzed by a Raspberry Pi computer to derive the ACR value (ALB and CRE concentrations). It is shown that the G + B value intensity signal is linearly related to the ALB and CRE concentrations with the correlation coefficients of R2 = 0.9919 and R2 = 0.9923, respectively. It is additionally shown that the ALB and CRE concentration results determined using the proposed method for 23 urine samples were collected from real suffering chronic kidney disease (CKD) patients are in fine agreement with those acquired operating a traditional high-reliability macroscale method. Overall, for point-of-care (POC) CKD diagnosis and monitoring in clinical applications, the results prove that the proposed method offers a convenient, real time, reliable, and low-spending solution for POC CKD diagnosis.


Assuntos
Creatina , Insuficiência Renal Crônica , Albuminas/análise , Creatinina/urina , Humanos , Microfluídica , Sistemas Automatizados de Assistência Junto ao Leito , Insuficiência Renal Crônica/diagnóstico , Reprodutibilidade dos Testes
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