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1.
Porto Alegre; Editora Rede Unida; 20220704. 277 p.
Monografia em Português | LILACS | ID: biblio-1378838

RESUMO

A Atenção Primária é sabidamente o pilar de todo sistema de atenção à saúde que aspira à eficiência e à cobertura universal. No Brasil, o Sistema Único de Saúde (SUS), embora operando com um padrão de financiamento ainda restritivo, garante esse horizonte de trabalho com uma capilaridade, cobertura multidisciplinar e resultados que o tornam referência para muitas outras nações. Seu desenvolvimento depende, dentre tantos fatores, de um trabalho contínuo de diagnóstico da realidade do país, ou mais precisamente das realidades diversas do país, e da avaliação da execução de seus programas e ações. Neste ponto, entre outros, o SUS e as Universidades atuam de modo convergente e integrado. A experiência acumulada do trabalho executado no sistema público de saúde gera uma agenda de investigações para pesquisadores e a pesquisa científica realizada no âmbito das instituições acadêmicas torna-se insumo para o aperfeiçoamento das políticas públicas em diferentes contextos. Este livro consolida contribuições de estudos da Atenção Básica à Saúde no estado do Pará, conduzidos no contexto do Programa Nacional de Melhoria do Acesso e da Qualidade da Atenção Básica (PMAQ-AB) por docentes, discentes e técnicos (as) da Universidade Federal do Pará (UFPA), participantes do Grupo de Estudo e Pesquisa em Saúde Coletiva na Amazônia (GEPESCA/UFPA). A realidade investigada é complexa e diversa, como o território e o perfil populacional amazônicos e suas correspondentes demandas por serviços de saúde. O retrato produzido informa sobre as exigências peculiares a que estão expostos os profissionais da saúde, assim como alguns dos obstáculos frequentes ao atendimento de qualidade na região. Nestes tempos de crise sanitária, humanitária, econômica, social sem precedentes, com mais de 620 mil mortes por Covid-19 no Brasil, em grande parte decorrentes da ação de um governo negacionista e neoliberal que promoveu a disseminação do vírus e da discórdia, debruçar-se sobre estes resultados de um trabalho dedicado ao fortalecimento do SUS é um alento.


Assuntos
Atenção Primária à Saúde , Serviços Básicos de Saúde , Política de Saúde , Política Pública , Sistema Único de Saúde , Atenção à Saúde , Crescimento e Desenvolvimento , Serviços de Saúde
2.
Int J Mol Sci ; 23(13)2022 Jul 05.
Artigo em Inglês | MEDLINE | ID: mdl-35806471

RESUMO

Although histone lysine methylation has been studied in thale cress (Arabidopsis thaliana (L.) Heynh.) and rice (Oryza sativa L.) in recent years, its function in maize (Zea mays L.) remains poorly characterized. To better understand the function of histone lysine methylation in maize, SDG102, a H3 lysine 36 (H3K36) methylase, was chosen for functional characterization using overexpressed and knockout transgenic plants. SDG102-deficiency in maize caused multiple phenotypes including yellow leaves in seedlings, late-flowering, and increased adult plant height, while the overexpression of SDG102 led to reduced adult plant height. The key flowering genes, ZCN8/ZCN7 and MADS4/MADA67, were downregulated in SDG102-deficient plants. Chromatin immunoprecipitation (ChIP) experiments showed that H3 lysine 36 trimethylation (H3K36me3) levels were reduced at these loci. Perturbation of SDG102 expression caused the misexpression of multiple genes. Interestingly, the overexpression or knockout of SDG102 also led to genome-wide decreases and increases in the H3K36me3 levels, respectively. Together, our results suggest that SDG102 is a methyltransferase that catalyzes the trimethylation of H3K36 of many genes across the maize genome, which are involved in multiple biological processes including those controlling flowering time.


Assuntos
Arabidopsis , Fenômenos Biológicos , Oryza , Arabidopsis/genética , Regulação da Expressão Gênica de Plantas , Crescimento e Desenvolvimento , Histona-Lisina N-Metiltransferase/genética , Histona-Lisina N-Metiltransferase/metabolismo , Histonas/genética , Histonas/metabolismo , Lisina/metabolismo , Oryza/genética , Zea mays/genética , Zea mays/metabolismo
4.
Proc Natl Acad Sci U S A ; 119(30): e2122148119, 2022 Jul 26.
Artigo em Inglês | MEDLINE | ID: mdl-35858396

RESUMO

In Darwin's and Mendel's times, researchers investigated a wealth of organisms, chosen to solve particular problems for which they seemed especially well suited. Later, a focus on a few organisms, which are accessible to systematic genetic investigations, resulted in larger repertoires of methods and applications in these few species. Genetic animal model organisms with large research communities are the nematode Caenorhabditis elegans, the fly Drosophila melanogaster, the zebrafish Danio rerio, and the mouse Mus musculus. Due to their specific strengths, these model organisms have their strongest impacts in rather different areas of biology. C. elegans is unbeatable in the analysis of cell-to-cell contacts by saturation mutagenesis, as worms can be grown very fast in very high numbers. In Drosophila, a rich pattern is generated in the embryo as well as in adults that is used to unravel the underlying mechanisms of morphogenesis. The transparent larvae of zebrafish are uniquely suited to study organ development in a vertebrate, and the superb versatility of reverse genetics in the mouse made it the model organism to study human physiology and diseases. The combination of these models allows the in-depth genetic analysis of many fundamental biological processes using a plethora of different methods, finally providing many specific approaches to combat human diseases. The plant model Arabidopsis thaliana provides an understanding of many aspects of plant biology that might ultimately be useful for breeding crops.


Assuntos
Arabidopsis , Crescimento e Desenvolvimento , Modelos Animais , Animais , Arabidopsis/genética , Arabidopsis/crescimento & desenvolvimento , Caenorhabditis elegans/genética , Drosophila melanogaster/genética , Pesquisa em Genética , Crescimento e Desenvolvimento/genética , Humanos , Camundongos , Melhoramento Vegetal , Peixe-Zebra/genética
5.
Nature ; 607(7919): 604-609, 2022 07.
Artigo em Inglês | MEDLINE | ID: mdl-35831506

RESUMO

Chromosome segregation errors during cell divisions generate aneuploidies and micronuclei, which can undergo extensive chromosomal rearrangements such as chromothripsis1-5. Selective pressures then shape distinct aneuploidy and rearrangement patterns-for example, in cancer6,7-but it is unknown whether initial biases in segregation errors and micronucleation exist for particular chromosomes. Using single-cell DNA sequencing8 after an error-prone mitosis in untransformed, diploid cell lines and organoids, we show that chromosomes have different segregation error frequencies that result in non-random aneuploidy landscapes. Isolation and sequencing of single micronuclei from these cells showed that mis-segregating chromosomes frequently also preferentially become entrapped in micronuclei. A similar bias was found in naturally occurring micronuclei of two cancer cell lines. We find that segregation error frequencies of individual chromosomes correlate with their location in the interphase nucleus, and show that this is highest for peripheral chromosomes behind spindle poles. Randomization of chromosome positions, Cas9-mediated live tracking and forced repositioning of individual chromosomes showed that a greater distance from the nuclear centre directly increases the propensity to mis-segregate. Accordingly, chromothripsis in cancer genomes9 and aneuploidies in early development10 occur more frequently for larger chromosomes, which are preferentially located near the nuclear periphery. Our findings reveal a direct link between nuclear chromosome positions, segregation error frequencies and micronucleus content, with implications for our understanding of tumour genome evolution and the origins of specific aneuploidies during development.


Assuntos
Aneuploidia , Posicionamento Cromossômico , Segregação de Cromossomos , Cromossomos , Proteína 9 Associada à CRISPR , Linhagem Celular , Linhagem Celular Tumoral , Segregação de Cromossomos/genética , Cromossomos/genética , Cromossomos/metabolismo , Cromotripsia , Crescimento e Desenvolvimento/genética , Humanos , Interfase , Micronúcleos com Defeito Cromossômico , Mitose , Neoplasias/genética , Neoplasias/patologia , Organoides/citologia , Organoides/metabolismo , Análise de Sequência de DNA , Análise de Célula Única
6.
Fungal Biol ; 126(6-7): 429-437, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35667830

RESUMO

Polyamines are ubiquitous polycationic molecules with multiple effects. Spermidine was present in all the life stages of Phycomyces blakesleeanus, fulfilled the physiological requirement for polyamines during germination, and became most abundant at the emergence of germinating tubes. Putrescine was not found in resting spores or in stationary cultures, but was synthesized during apical growth and greatly exceeded spermidine in fast-growing stages of the vegetative and sexual life cycles. Changes in the polyamines did not correlate with the various stages of sporulation. Ornithine decarboxylase was so strongly inhibited in vitro by its product, putrescine, that it would completely block the enzyme if not compartmentalized away. 1,4-Diamino-2-butanone inhibited mycelial growth throughout the vegetative cycle without killing the cells. The inhibition was counteracted very effectively by putrescine, which acts as a close analog of the inhibitor, and very little by spermidine. Four independent spe mutants were obtained by a procedure that selected for resistance to diaminobutanone among functionally-uninucleate spores that survived exposure to N-methyl-N'-nitro-N-nitrosoguanidine. The stability of the enzyme, in vivo and in vitro, and its inhibition by diaminobutanone in vitro were the same in the wild type and in the mutants. Two of these were hypomorph mutants, with lower affinity of their ornithine decarboxylase for its substrate, ornithine, and lower maximal velocity. The other two were hypermorph transport mutants; we propose that they are affected in a protein that binds putrescine and its analogs for transport across the plasmalemma and sequestration away from the active enzyme. The transport mutants concentrated the exogenous diaminobutanone and the endogenous putrescine in inactive compartments; the highest enzyme activity was reached when the plasmalemma of the mutants was permeabilized with diethylaminoethyl dextran.


Assuntos
Ornitina Descarboxilase , Poliaminas , Crescimento e Desenvolvimento , Ornitina Descarboxilase/genética , Ornitina Descarboxilase/metabolismo , Phycomyces , Poliaminas/metabolismo , Putrescina/farmacologia , Espermidina/farmacologia
7.
Zhonghua Wai Ke Za Zhi ; 60(6): 558-566, 2022 Jun 01.
Artigo em Chinês | MEDLINE | ID: mdl-35658343

RESUMO

Objective: To explore the effect of atlantoaxial fusion on the growth and development of children's cervical spine. Methods: The clinical data of 12 children with atlantoaxial dislocation who underwent posterior atlantoaxial fusion at Department of Orthopaedics,the 909th Hospital of Joint Logistics Support Force of Chinese People's Liberation Army from June 2002 to September 2013 were retrospective analyzed. There were 7 males and 5 females,with age of (8.1±3.1)years (range:3 to 13 years).Nine cases were traumatic and 3 cases were congenital malformations,1 of the children had Down syndrome. All children underwent posterior atlantoaxial fusion. Furthermore,the information of the height and anteroposterior width of the cervical vertebral bodies and vertical growth rate of the fusion mass were collected from all patients immediately postoperatively and during the follow-up.The range of motion in cervical spine were collected preoperatively and during follow-up period. Data were compared using independent sample t test, paired sample t test and repeated-measurement. Results: All 12 children had regular follow-up within (122.4±25.3)months(range:65 to 163 months). The height and anteroposterior width of the cervical vertebral bodies were similar to these results with those in published reports of growth in normal children of the same age(all P<0.01). At the last follow-up,atlantoaxial fusion of 11 cases had substantial growth (vertical growth rate of the fusion mass:11 cases ≥10%, 1 case <10%);the range of motion in cervical spine was close to the normal level (flexion(55.2±5.0)°,extension (65.3±4.9)°,left bending (41.7±4.5)°,right bending (42.4±4.4)°,left rotation (66.4±5.6)°,right rotation (68.5±5.8)°). Conclusions: Atlantoaxial fusion surgery is satisfactory in the treatment of pediatric atlantoaxial dislocation.During the follow-up,the growth and development of the cervical spine is close to that of normal children of the same age.In long-term observation,it has been found that the operation has no negative effect on the growth and development of the children's cervical spine.


Assuntos
Articulação Atlantoaxial , Luxações Articulares , Fusão Vertebral , Articulação Atlantoaxial/anormalidades , Articulação Atlantoaxial/cirurgia , Vértebras Cervicais/cirurgia , Criança , Anormalidades Congênitas , Feminino , Crescimento e Desenvolvimento , Humanos , Luxações Articulares/cirurgia , Masculino , Estudos Retrospectivos , Fusão Vertebral/métodos , Resultado do Tratamento
8.
PLoS One ; 17(6): e0269446, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35657910

RESUMO

Green roof systems, aimed at reducing anthropic impact on the environment, are considered environmental mitigation technologies and adopted by many countries across the world to strengthen urban ecosystem services. This study evaluates two mixtures of succulent: one of Crassulaceae and the other of Aizoaceae, used in the creation of a continuous and homogenous plant groundcover in Mediterranean environments. To assess the species mixtures, the parameters plant height, growth index, cover percentage and flowering were observed. Hydrological observations were also carried out to evaluate the rainfall retained by the test system in any given month. All data were subjected to analysis of variance. Growth indicators in the study showed trends characteristic of xeric plants, which tend to slow down in dry, summer climate conditions to the point of halting plant vertical growth and ground cover development completely. The Aizocaeae mix, during the initial stage, showed prevalent horizontal growth, confirmed by greater a greater growth index (13,21) and cover percentage (45%) compared to Sedum (Growth index: 3,61; Cover: 36%). In contrast, the Sedum mix recorded greater vertical growth at the beginning (Sedum mixture: 7.53 cm; Aizoaceae mixture: 6,11 cm). During the final stages of observations, however, greater vertical growth in the Aizoaceae (7,88 cm) became apparent together with a recovery in horizontal growth in the Sedum (79%), albeit not sufficient to outperform the Aizoaceae mixture (87%). Flowering in the two mixtures occurred between late spring and late summer. The Sedum mixture guaranteed a longer flowering period (130 days) compared to the Aizoaceae (93 days), with a gradual start followed by steady flower emission. Regarding rainfall water retention, a comparison of the mixtures in late winter/early spring revealed that the Sedum performed best (44.9 L m2 vs 37.4 L m2), whilst the Aizoaceae outperformed the Sedum in Autumn (63 L m2 vs 55 L m2), in conjunction with favorable growth rates in both species mixtures. Both mixtures demonstrated satisfying results and are considered suited to a Mediterranean environment. Furthermore, based on the different growth rates of the species in the two test mixtures, this study suggests that new combinations of Sedum and Aizoaceae together might prove more resilient in Mediterranean environments.


Assuntos
Ecossistema , Sedum , Clima , Conservação dos Recursos Naturais/métodos , Crescimento e Desenvolvimento , Plantas
9.
Front Public Health ; 10: 898266, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35712283

RESUMO

For children aged 7-8 years in China, "immobility" is a key problem hindering their physical and mental development in recent years. It is widely accepted that motor performance development in children is accompanied by physical and mental growth and development. However, few studies have clarified the relationship among motor behavior, motor development and motor performance. To bridge this knowledge gap, an empirical analysis of children aged 7-8 years in China was conducted. This study developed scales for testing motor performance, motor behavior and motor performance, respectively, and collected data of these tests on children aged 7-8 years in China. Canonical correlation analysis was used to analyze the correlations among motor performance, motor behavior and motor performance, and partial least squares regression was used to evaluate the relationship between dependent and independent variables. It was found that, for the children aged 7-8 years in China, there were significant positive correlations among the motor performance, motor behavior, and motor development. The three tests were closely related and could be applied to promote children's sports performance through improved training activities for targeting specific indicators. The study found there was no significant differences in the application of the three tests for children with different age and gender. This finding lays a foundation for further testing in older children and meets the measurement requirements of modern medicine's "bio-psycho-social model of health promotion". Additionally, the theoretical motional quotient model of "The Bio-Behavior-Task" is constructed as a comprehensive motor performance evaluation system, aligning with students' physical and mental development standards.


Assuntos
Desempenho Atlético , Estudantes , Criança , China , Crescimento e Desenvolvimento , Humanos
10.
Rev. SPAGESP ; 23(1): 103-116, jan.-jun. 2022. tab
Artigo em Português | LILACS, Index Psicologia - Periódicos | ID: biblio-1356773

RESUMO

RESUMO O uso das mídias digitais por bebês é um fenômeno recente. Algumas evidências apontam para associações do uso de telas na primeira infância e desfechos adversos no desenvolvimento. O objetivo deste estudo é apresentar o processo de construção, implementação e avaliação do estudo-piloto de uma intervenção educativa para pais, profissionais da saúde e educadores sobre uso de mídias digitais na primeira infância. A intervenção segue o modelo de educação continuada, consistiu em quatro encontros presenciais no formato expositivo seguido de discussão em grupo e foi avaliada em termos da aprendizagem dos participantes e satisfação com o curso. Participaram 16 pessoas, majoritariamente profissionais de saúde e educação. Os resultados indicaram melhora significativa nos conhecimentos do tema e satisfação com a intervenção, sendo discutidos de forma a subsidiar o desenvolvimento de novas intervenções sobre a temática.


ABSTRACT The use of digital media by babies is a recent phenomenon. Some evidence highlights associations between the use of screens in early childhood and adverse developmental outcomes. This study aims to report the development, implementation, and evaluation processes of an educational intervention for parents, health professionals, and educators on the use of digital media in early childhood. The intervention follows the model of continuing education, with four face-to-face meetings and presentations followed by group discussions. It was evaluated by participants' learning and satisfaction with the course. Sixteen people participated, mostly health and education professionals. The results indicate a significant improvement in participants’ knowledge and high satisfaction rates. Findings are discussed to support the development of new interventions on the subject.


RESUMEN El uso de medios digitales por parte de los bebés es un fenómeno reciente. Algunas evidencias apuntan asociaciones entre el uso de pantallas en la primera infancia y los resultados adversos del desarrollo. El objetivo del presente estudio es presentar el proceso de construcción, implementación y evaluación del estudio piloto de una intervención educativa para padres, profesionales de la salud y educadores sobre el uso de medios digitales en la primera infancia. La intervención sigue el modelo de educación continua y consistió en cuatro reuniones presenciales en formato expositivo, seguidas de discusión grupal y aún fue evaluada en términos del aprendizaje y satisfacción de los participantes con el curso. Participaron 16 personas, en su mayoría identificadas como profesionales de la salud y de la educación. Los resultados indicaron una mejora significativa en el conocimiento del tema y satisfacción con la intervención, siendo discutida con el fin de apoyar el desarrollo de nuevas intervenciones sobre el tema.


Assuntos
Humanos , Lactente , Pré-Escolar , Pais , Desenvolvimento Infantil , Crescimento e Desenvolvimento , Mídia Audiovisual , Aprendizagem
11.
Trials ; 23(1): 505, 2022 Jun 16.
Artigo em Inglês | MEDLINE | ID: mdl-35710445

RESUMO

BACKGROUND: Globally, household air pollution (HAP) is a leading environmental cause of morbidity and mortality. Our trial aims to assess the impact of liquefied petroleum gas (LPG) for cooking to reduce household air pollution exposure on child health outcomes, compared to usual cooking practices in Bangladesh. The primary aim is to evaluate if reduced exposure to HAP through the provision of LPG for cooking from early gestation through to age 2 improves child anthropometry, health, and neuro-cognitive developmental outcomes, compared to children exposed to emissions from usual practice. METHODS: Two-arm parallel cluster randomized controlled trial (cCRT). We will extend the intervention and follow-up of our existing "Poriborton" trial. In a subset of the original surviving participants, we will supply LPG cylinders and LPG stoves (intervention) compared to usual cooking practices and extend the follow-up to 24 months of age. The expected final sample size, for both (intervention and control) is 1854 children with follow-up to 2 years of age available for analysis. DISCUSSION: This trial will answer important research gaps related to HAP and child health and neuro-cognitive developmental outcomes. This evidence will help to understand the impact of a HAP intervention on child health to inform policies for the adoption of clean fuel in Bangladesh and other similar settings. TRIAL REGISTRATION: The Poriborton: Change trial: Household Air Pollution and Perinatal and early Neonatal mortality is registered with the Australian New Zealand Clinical Trials Registry, ACTRN12618001214224, original trial registered on 19th July 2018, extension approved on 23rd June 2021. www.anzctr.org.au .


Assuntos
Poluição do Ar em Ambientes Fechados , Poluição do Ar , Utensílios Domésticos , Petróleo , Poluição do Ar/análise , Poluição do Ar em Ambientes Fechados/efeitos adversos , Poluição do Ar em Ambientes Fechados/prevenção & controle , Austrália , Criança , Pré-Escolar , Culinária , Feminino , Crescimento e Desenvolvimento , Humanos , Recém-Nascido , Gravidez , Ensaios Clínicos Controlados Aleatórios como Assunto
12.
Rev Saude Publica ; 56: 56, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35766785

RESUMO

OBJECTIVE: To analyze the impact of the covid-19 pandemic on the use of primary health care services to follow-up the child growth and development in Brazil. METHOD: A total of 7.9 million consultations of children (0-2 years old) across Brazil between March 2017 and May 2020 were studied. Differences between medians were analyzed using non-parametric tests, the Global Moran Index (IGM) and the Local Indicators of Spatial Association. RESULTS: During the initial period of the pandemic, the median number of consultations was significantly lower than the same period in previous years, reducing more than 50%. The drop in 2020, compared to 2019, ranged from 49% to 62.2% across all regions of the country, except the South. The percentage reduction registered in 2019-2020 showed significant spatial autocorrelation (IGM = 0.20; p = 0.04), with the presence of low-low (states with reduction between 29% and 51%) and high-high (states with reduction between 55% and 69%) spatial clusters. CONCLUSION: The covid-19 pandemic impacted the follow-up of child growth and development in primary health care services in Brazil, with a geographically uneven reduction.


Assuntos
COVID-19 , Brasil/epidemiologia , COVID-19/epidemiologia , Criança , Pré-Escolar , Seguimentos , Crescimento e Desenvolvimento , Humanos , Imunoglobulina M , Lactente , Recém-Nascido , Pandemias
13.
Sci Rep ; 12(1): 9272, 2022 06 03.
Artigo em Inglês | MEDLINE | ID: mdl-35661116

RESUMO

The use of complementary light spectra is a potential new approach to studying the increase in plant resilience under stress conditions. The purpose of this experiment was to investigate the effect of different spectra of complementary light on the growth and development of strawberry plants under salinity and alkalinity stress conditions. Plants were grown in the greenhouse under ambient light and irradiated with blue (460 nm), red (660 nm), blue/red (1:3), and white/yellow (400-700 nm) light during the developmental stages. The stress treatments were as follows: control (non-stress), alkalinity (40 mM NaHCO3), and salinity (80 mM NaCl). Our results showed that salinity and alkalinity stress decreased fresh and dry weights and the number of green leaves, and increased chlorotic, tip burn, and dry leaves. The blue and red spectra had a greater effect on reducing the effects of stress compared to other spectra. Stress conditions decreased SPAD and RWC, although blue light increased SPAD, and blue/red light increased RWC under stress conditions. Blue/red and white/yellow light had the greatest effect on reproductive traits. Stress conditions affected fruit color indicators, and red and blue light had the most significant effect on these traits. Under stress conditions, sodium uptake increased, while K, Ca, Mg, and Fe uptake decreased, markedly. Blue and red light and their combination alleviated this reducing effect of stress. It can be concluded that the effects of salinity and alkalinity stresses can be reduced by manipulating the supplemental light spectrum. The use of artificial light can be extended to stresses.


Assuntos
Fragaria , Crescimento e Desenvolvimento , Luz , Folhas de Planta , Salinidade
14.
Postepy Biochem ; 68(1): 46-56, 2022 03 31.
Artigo em Inglês | MEDLINE | ID: mdl-35569044

RESUMO

In plants, lipids serve as one of the major and vital cellular constituents. Neutral lipids reserves play an essential role in the plant life cycle by providing carbon and energy equivalents for periods of active metabolism. The most common form of lipid storage are triacylglycerols (TAGs) packed into specialized organelles called lipid droplets (LDs). They have been observed in diverse plant organs and tissues, like oil seeds or pollen grains. LDs consist of a core, composed mostly of TAGs, enclosed by a single layer of phospholipids that is decorated by a unique set of structural proteins. Moreover, the recent advances in exploration of LDs proteome revealed a plethora of diverse proteins interacting with LDs. This is likely the result of a highly dynamic nature of these organelles and their involvement in many diverse aspect of cellular metabolism, tightly synchronized with plant developmental programs and directly related to plant-environment interactions. In this review we summarize and discuss the current progress in understanding the role of LDs and their cargo during plants life cycle, with a special emphasis on developmental aspects.


Assuntos
Gotículas Lipídicas , Plantas , Crescimento e Desenvolvimento , Gotículas Lipídicas/química , Gotículas Lipídicas/metabolismo , Metabolismo dos Lipídeos , Lipídeos/análise , Plantas/metabolismo , Proteoma/metabolismo
15.
J Oral Rehabil ; 49(9): 915-923, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-35583904

RESUMO

BACKGROUND: Unilateral posterior crossbite, one of the most frequent malocclusions, is often associated with functional lateral shift of the mandible. Although the effects of functional lateral shift on the mandible and temporomandibular joint have been examined in various animal experiments, cranial and maxillary changes have received less attention. OBJECTIVE: The aim of this study was to investigate the effects of functional lateral shift on the craniofacial complex in growing rats. METHODS: Eighty 5-week-old male Sprague-Dawley rats were randomly divided into an experimental group (n = 40), which received an oblique guide appliance that shifted the mandible to the left during closure, and a control group (n = 40). The rats were scanned by cone-beam computed tomography at 3 days and 1, 2, 4 and 8 weeks. The dimensions of the mandibular bone, condyle, maxilla and cranium were measured. RESULTS: The mandibles of rats in the experimental group were smaller than those of the rats in the control group and were asymmetrical. The condyles of the rats in the experimental group were thinner than those of the control rats. The condylar length on the ipsilateral side was shorter and wider than that on the contralateral side from 4 to 8 weeks. No significant differences in cranial length or height were observed between the experimental and control groups. The height of the upper first molar and alveolar bone on the contralateral side was significantly smaller than that on the ipsilateral side and in the controls from 4 to 8 weeks. CONCLUSION: Functional shift in the mandible produces morphological asymmetries in the mandible and maxillary region and may cause bilateral condylar degenerative changes.


Assuntos
Assimetria Facial , Má Oclusão , Animais , Assimetria Facial/complicações , Crescimento e Desenvolvimento , Masculino , Mandíbula/diagnóstico por imagem , Côndilo Mandibular/diagnóstico por imagem , Ratos , Ratos Sprague-Dawley
16.
Parasit Vectors ; 15(1): 177, 2022 May 24.
Artigo em Inglês | MEDLINE | ID: mdl-35610663

RESUMO

BACKGROUND: Schistosomiasis, an acute and chronic parasitic disease, causes substantial morbidity and mortality in tropical and subtropical regions of the world. Iron is an essential constituent of numerous macromolecules involving in important cellular reactions in virtually all organisms. Trematodes of the genus Schistosoma live in iron-rich blood, feed on red blood cells and store abundant iron in vitelline cells. Ferritins are multi-meric proteins that store iron inside cells. Three ferritin isoforms in Schistosoma japonicum are known, namely SjFer0, SjFer1 and SjFer2; however, their impact on the growth and development of the parasites  is still unknown. In this study we report on and characterize the ferritins in S. japonicum. METHODS: A phylogenetic tree of the SjFer0, SjFer1 and SjFer2 genes was constructed to show the evolutionary relationship among species of genus Schistosoma. RNA interference in vivo was used to investigate the impact of SjFer0 on schistosome growth and development.  Immunofluorescence assay was applied  to localize the expression of the ferritins. RNA-sequencing was performed  to characterize the iron transport profile after RNA interference. RESULTS: SjFer0 was found to have low similarity with SjFer1 and SjFer2 and contain an additional signal peptide sequence. Phylogenetic analysis revealed that SjFer0 can only cluster with some ferritins of other trematodes and tapeworms, suggesting that this ferritin branch might be unique to these parasites. RNA interference in vivo showed that SjFer0 significantly affected the growth and development of schistosomula but did not affect egg production of adult female worms. SjFer1 and SjFer2 had no significant impact on growth and development. The immunofluorescence study showed that SjFer0 was widely expressed in the somatic cells and vitelline glands but not in the testicle or ovary. RNA-sequencing indicated  that, in female, the ion transport process and calcium ion binding function were downregulated after SjFer0 RNA interference. Among the differentially downregulated genes, Sj-cpi-2, annexin and insulin-like growth factor-binding protein may be accounted for the suppression of schistosome growth and development. CONCLUSIONS: The results indicate that SjFer0 affects the growth and development of schistosomula but does not affect egg production of adult female worms. SjFer0 can rescue the growth of the fet3fet4 double mutant Saccharomyces cerevisiae (strain DEY1453), suggesting being able to promote iron absorption. The RNA interference of SjFer0 inferred that  the suppression of worm growth and development may via  down-regulating Sj-cpi-2, annexin, and IGFBP.


Assuntos
Schistosoma japonicum , Esquistossomose Japônica , Animais , Anexinas/genética , Feminino , Ferritinas/genética , Crescimento e Desenvolvimento , Ferro/metabolismo , Filogenia , RNA/metabolismo , Schistosoma japonicum/fisiologia , Esquistossomose Japônica/parasitologia
17.
Environ Toxicol Chem ; 41(7): 1686-1695, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35611556

RESUMO

Recently, the high toxicity of neonicotinoids to the survival and reproduction of adult earthworms has become apparent in standard 56-day toxicity tests. The persistence of some neonicotinoids and/or their repeated application may lead to long-term exposure, possibly also affecting other parts of the life cycle of earthworms. The present study aimed at providing insight into the sublethal effects of imidacloprid, thiacloprid, and clothianidin on juvenile Eisenia andrei exposed for 16 weeks in Lufa 2.2 soil. Significant effects on growth and maturation were observed for all compounds. Exposure to 0.125 mg imidacloprid/kg dry soil and 0.03125 and 0.0625 mg thiacloprid/kg dry soil significantly affected the growth of the earthworms, while significant maturation effects were observed at 0.03125 mg/kg dry soil for imidacloprid and thiacloprid and 0.25 mg clothianidin/kg dry soil. The 16-week no-observed-effect concentrations (NOECs) found in the present study were lower than previously reported NOECs for effects on earthworm reproduction. Predicted environmental concentrations after a single application exceeded the observed NOECs for effects on earthworm maturation in the case of imidacloprid and thiacloprid and for effects on earthworm growth in the case of thiacloprid and clothianidin. Environ Toxicol Chem 2022;41:1686-1695. © 2022 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.


Assuntos
Inseticidas , Oligoquetos , Poluentes do Solo , Animais , Crescimento e Desenvolvimento , Guanidinas , Inseticidas/química , Neonicotinoides/toxicidade , Nitrocompostos , Solo/química , Poluentes do Solo/química , Poluentes do Solo/toxicidade , Tiazinas , Tiazóis
18.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao ; 44(2): 236-243, 2022 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-35538758

RESUMO

Objective To understand the growth and dietary diversity status of children in multi-ethnic areas of Sichuan province,and to explore the associations of dietary diversity with growth and development indicators. Methods Children of 18-36 months old and their primary caregivers were selected with multi-stage cluster random sampling method from rural areas of Han,Tibetan,and Yi ethnic groups in Sichuan province. The sociodemographic information of children and their caregivers was collected using self-designed questionnaire.The dietary diversity score(DDS)was calculated according to the criteria in the Guidelines for Measuring Household and Individual Dietary Diversity released by the Food and Agriculture Organization of the United Nations.The body height(length)and body weight of each child were measured by standard equipment for anthropological measurement,and the height for age Z score(HAZ),weight for age Z score(WAZ),and weight for height Z score(WHZ) were calculated.Multivariate linear regression was performed to analyze the relationship between dietary diversity and growth indicators of children. Results A total of 1092 children were enrolled in this study,and the prevalence of stunting(HAZ<-2),underweight(WAZ<-2),and wasting(WHZ<-2)was 21.1%,4.9%,and 2.5%,respectively.The children had the mean DDS of 4.8±1.7,and 45.3% of children had poor dietary diversity(DDS≤4).The children of Han ethnic group(5.8±1.4)had higher DDS than those of Tibetan ethnic group(4.9±1.6)and Yi ethnic group(3.9±1.6)(P<0.001).The results of multivariate linear regression indicated that DDS was positively correlated with HAZ(ß=0.206,95%CI=0.158-0.254,P<0.001)after adjustment of sex,age,birth weight,preterm birth,and parental body height.After further adjustment of family fixed assets,ethnic group,caregiver's type,and caregiver's education background,the correlation between DDS and HAZ remained significant(ß=0.077,95%CI=0.026-0.128, P=0.003). Conclusions The children in the multi-ethnic rural areas of Sichuan province showed troublesome growth and development status and low dietary diversity,which were conspicuously different between ethnic groups,especially in the rural areas of Yi ethnic group.The dietary diversity was positively associated with HAZ.It is recommended to carry out nutrition and health education according to the local dietary characteristics and thus improve the growth and development of children in multi-ethnic rural areas in Sichuan.


Assuntos
Nascimento Prematuro , Criança , Pré-Escolar , China/epidemiologia , Feminino , Crescimento e Desenvolvimento , Humanos , Lactente , Recém-Nascido , Estado Nutricional , Gravidez , População Rural
19.
Plant Cell Rep ; 41(8): 1631-1650, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-35575808

RESUMO

Melatonin has attracted widespread attention after its discovery in higher plants. Tomato is a key model economic crop for studying fleshy fruits. Many studies have shown that melatonin plays important role in plant stress resistance, growth, and development. However, the research progress on the role of melatonin and related mechanisms in tomatoes have not been systematically summarized. This paper summarizes the detection methods and anabolism of melatonin in tomatoes, including (1) the role of melatonin in combating abiotic stresses, e.g., drought, heavy metals, pH, temperature, salt, salt and heat, cold and drought, peroxidation hydrogen and carbendazim, etc., (2) the role of melatonin in combating biotic stresses, such as tobacco mosaic virus and foodborne bacillus, and (3) the role of melatonin in tomato growth and development, such as fruit ripening, postharvest shelf life, leaf senescence and root development. In addition, the future research directions of melatonin in tomatoes are explored in combination with the role of melatonin in other plants. This review can provide a theoretical basis for enhancing the scientific understanding of the role of melatonin in tomatoes and the improved breeding of fruit crops.


Assuntos
Lycopersicon esculentum , Melatonina , Secas , Crescimento e Desenvolvimento , Lycopersicon esculentum/fisiologia , Melhoramento Vegetal , Plantas , Estresse Fisiológico
20.
Rev. latinoam. cienc. soc. niñez juv ; 20(1): 303-327, ene.-abr. 2022. tab, graf
Artigo em Espanhol | LILACS | ID: biblio-1365876

RESUMO

Resumen (analítico) El proyecto de vida de la juventud rural colombiana transita por dificultades en su planeación y desarrollo. Por ende, este estudio busca identificar los factores que interfieren en la construcción del proyecto de vida en jóvenes rurales, con una metodología de investigación cualitativa, enfoque etnometodológico y diseño transversal. Se emplearon como técnicas de recolección de información entrevistas semies tructuradas, grupos focales, observación y análisis documental. Participaron 117 adolescentes, ocho docentes y seis padres de familia. Los resultados facilitaron comprender los factores individuales y sociales que inciden en el proyecto de vida de la juventud rural desde el modelo ecológico, que aborda la relación del adolescente rural con las características de su contexto y específicamente desde el microsistema, mesosistema, exosistema y macrosistema.


Abstract (analytical) The life projects of Colombian young people in rural areas are facing difficulties in terms of planning and implementation. This study sought to identify the factors that affect rural young people's construction of life projects. This involved the use of a qualitative research methodology, ethnomethodological approach and cross-sectional design. Semi-structured interviews, focus groups, participant observation and documentary analysis were selected as data collection techniques. A total of 117 adolescents, 8 teachers and 6 parents participated. Using the ecological model, the results facilitated understanding of the individual and social factors that influence the life projects of rural youth. The study identified the importance of the relationship of rural adolescents with the characteristics of their context and specifically the microsystems, mesosystems, exosystems and macrosystems they inhabit.


Resumo (analítico) O projeto de vida da juventude rural colombiana está passando por dificuldades em seu planejamento e desenvolvimento, portanto, este estudo procura identificar os fatores que interferem na construção do projeto de vida na juventude rural, com uma metodologia de pesquisa qualitativa, abordagem etnometodológica e desenho transversal. Foram utilizadas entrevistas semiestruturadas, grupos de foco, observação e análise documental como técnicas de coleta de dados. Participaram um total de 117 adolescentes, 8 professores e 6 pais participaram. Os resultados facilitaram uma compreensão dos fatores individuais e sociais que influenciam o projeto de vida dos jovens rurais a partir do modelo ecológico, que aborda a relação dos adolescentes rurais com as características de seu contexto e especificamente do microssistema, mesossistema, exossistema e macrossistema.


Assuntos
Zona Rural , Pesquisa Qualitativa , Crescimento e Desenvolvimento
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