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1.
PLoS One ; 17(5): e0266683, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35507581

RESUMO

Foodborne diseases have become a serious havoc, where antimicrobial resistance is throwing significant challenges on daily basis. With the increase of drug-resistant bacteria and food-borne infection associated with Vibrio parahaemolyticus, new and effective strategies were needed to control the emergence of vibriosis. Lytic bacteriophages come up as a promising way to resist the pathogenic population in various applications. In this study, a V. parahaemolyticus specific phage vB_VpS_PG28 was isolated from sewage in the seafood market. Results showed vB_VpS_PG28, is strictly a lytic bacteriophage and has a relatively large burst size of 103 plaque-forming units per infected cell. Comparative genomic and bioinformatic analyses proved that vB_VpS_PG28 is a new bacteriophage that had a homologous relation with Vibrio phages of family Siphoviridae, especially with phage VH2_2019, but transmission electron microscopy of vB_VpS_PG28 morphology characterized its morphology is similar to that of Myoviridae family. In silico analysis indicated that the vB_VpS_PG28 genome consists of 82712 bp (48.08% GC content) encoding 114 putative ORFs without tRNA,and any gene associated with resistance or virulence factors has not been found. The bacteriophage in the present study has shown significant outcomes in order to control bacterial growth under in vitro conditions. Thus, we are suggesting a beneficiary agent against foodborne pathogens. Further, to ensure the safe usage of phage oral toxicity testing is recommended.


Assuntos
Bacteriófagos , Vibrio parahaemolyticus , Animais , Crustáceos/genética , Genoma Viral , Filogenia , Alimentos Marinhos , Análise de Sequência , Vibrio parahaemolyticus/genética
2.
Molecules ; 27(7)2022 Apr 03.
Artigo em Inglês | MEDLINE | ID: mdl-35408719

RESUMO

We report here the purification of a novel metal-binding protein from Oratosquilla oratoria (O. oratoria MT-1) by gel and ion-exchange chromatography. SDS-PAGE and MALDI-TOF analyses demonstrated that isolated O. oratoria MT-1 was of high purity with a molecular weight of 12.4 kDa. The fluorescence response to SBD-F derivatives revealed that O. oratoria MT-1 contained a large number of sulfhydryl groups, which is a general property of metallothioneins. Zn and Cu metal stoichiometries for O. oratoria MT-1 were 3.97:1 and 0.55:1, respectively. The proportion of cysteine (Cys) residues in the amino acid composition was 32.69%, and aromatic amino acids were absent. The peptide sequence coverage with Macrobrachium rosenbergii calmodulin (accession AOA3S8FSK5) was 60%. Infrared spectroscopy of O. oratoria MT-1 revealed two obvious peaks at absorption frequencies for the amide I band and the amide II band. CD spectra revealed that the secondary structure was mainly composed of random coil (57.6%) and ß-sheet (39.9%). An evaluation of in vitro antioxidant activity revealed that isolated O. oratoria MT-1 has strong reducing activities, exhibiting scavenging rates for DPPH and OH of 77.8% and 75.8%, respectively (IC50 values 0.57 mg/mL and 1.1 mg/mL). O. oratoria MT-1 may be used as a functional additive in cosmetics, health foods, and medical products, as well as a reference material for quantitative analysis of metallothionein in such products.


Assuntos
Antioxidantes , Metalotioneína , Amidas , Animais , Antioxidantes/farmacologia , Crustáceos , Estrutura Secundária de Proteína
3.
J Fish Biol ; 100(4): 859, 2022 04.
Artigo em Inglês | MEDLINE | ID: mdl-35474575
4.
Zootaxa ; 5093(1): 83-93, 2022 Jan 25.
Artigo em Inglês | MEDLINE | ID: mdl-35390815

RESUMO

Neocypridella fossulata (Daday, 1910) is redescribed based on type material from the Daday collection (Hungarian Natural History Museum, Budapest). The major diagnostic characters of the genus are the ovate shape of the carapace in lateral view, with the left valve overlapping the right valve, the absence of marginal septa on the valve margins, the right valve anterior selvage not inwardly displaced, the presence of a Wouters organ on the first antenna, the divided penultimate segment of the second antenna, the markedly large -seta on the mandibular palp, the subquadrate terminal segment of the maxillula palp, the divided penultimate segment of the second thoracopod with setae d1 and d2 both long, the slender caudal ramus and the slender caudal ramus attachment with thin dorsal and ventral branches. Neocypridella fossulata can be further characterized by the oblique carapace in frontal view, the two smooth bristles on the maxillula third endite and the thin and long Ga claw of the caudal ramus, which is c. 3/5 of the length of the ramus. The morphology of Neocypridella is compared with related taxa and the taxonomic position of the genus is discussed. This revealed that Neocypridella cannot be assigned to any of the extant subfamilies in the Cyprididae. Therefore, a new subfamily, Neocypridellinae subfam. nov., is erected.


Assuntos
Crustáceos , Distribuição Animal , Animais
5.
Zootaxa ; 5100(3): 301-348, 2022 Feb 21.
Artigo em Inglês | MEDLINE | ID: mdl-35391072

RESUMO

We describe Malawidopsis gen. nov., a new genus of Cypridopsinae Kaufmann, 1900 from the African ancient Lake Malawi. The genus comprises at least 17 new species, which makes it a significant ostracod radiation in this lake, parallel to a similar (but independent) cypridopsine radiation in Lake Tanganyika. Three of these new species are here described: Malawidopsis stellae gen. et. sp. nov., the type species of the new genus; M. ruwaydae gen. et. sp. nov. and M. antoniae gen. et. sp. nov.. The other new species are briefly illustrated and described, but are left in open nomenclature (sp. A, B, C, etc.). Cypridopsis cunningtoni Sars, 1910 and Potamocypris fuelleborni Daday, 1910 are transferred to Malawidopsis gen. nov. and are identical to Malawidopsis spec. F and Malawidopsis spec. N, respectively. The new tribe Plesiocyprisopsini trib. nov. is erected, and comprises the cypridopsine genera previously in the Cypridopsini s.l. with the right valve overlapping the left valve, at least anteriorly. Potential drivers of speciation within this endemic clade in Lake Malawi are briefly discussed. Bathymetry might have been important, with most species being restricted to shallower depths and only four species also occurring at depths of 75 m or more, but very few specimens were retrieved from greater depths. Most species occurred on coarse sand, but this sediment category coincides with shallower stations. Overall, most species appear to have a wide geographical distribution in the lake, so no geographical parapatric speciation is apparent. The occurrence of all species in sexual populations and the significant differences in the male sexual organs and the valves suggest that sexual selection might have been the most important driver in the speciation process of this species flock, but this should be further explored. Following deep coring results in Lake Malawi, the present clade could be (at least) c one million years old.


Assuntos
Crustáceos , Lagos , Animais , Malaui , Masculino
6.
Zootaxa ; 5125(1): 75-91, 2022 Apr 07.
Artigo em Inglês | MEDLINE | ID: mdl-35391093

RESUMO

Nanomysis siamensis W.M. Tattersall, 1921 is a shallow water mysid which was first described from the Thale Luang and the Thale Sap waters, Songkhla Lagoon System in southern Thailand. Recently, this species was captured further south, in the Thale Sap Songkhla waters. In this study, we provide updated records on spatial distribution of the species in the Songkhla Lagoon System. A total of 272 individuals of this species were observed, and illustrations of the body including all appendages are presented. Among them, the mandible, maxillule and maxilla are described for the first time. Morphological characteristics of N. siamensis are compared with other two known species of the genus Nanomysis.


Assuntos
Crustáceos , Mandíbula , Animais , Extremidades , Tailândia
7.
Zootaxa ; 5116(2): 200-222, 2022 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-35391338

RESUMO

Two new genera of parapseudid Tanaidacea belonging to the Discapseudes-Halmyrapseudes Complex sensu Heard et al. (=Complex) are designated from Mexican and Australian waters. Mexctenapseudes boeschi n. gen., n. sp. is described from Campeche Mexico and Pseudolongiflagrum n. gen. is designated from Australia. The new Mexican genus displays similarities to the West Pacific and Indian Ocean genera Ctenapseudes Bamber, Ariyananda Silva, 1997 and Longiflagrum Guu, 1995. The new related genus, Pseudolongiflagrum, having affinities with Ctenapseudes and Mexctenapseudes n. gen., is designated to receive Longiflagrum caeruleus (Boesch) known from Australia. A key to the genera within the parapseudid subfamily Parapseudinae Guu is presented and possible factors concerning the ecology, origins, and distribution of the members of the Complex considered.


Assuntos
Crustáceos , Meio Ambiente , Animais , Austrália , México
8.
Zootaxa ; 5116(2): 151-199, 2022 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-35391339

RESUMO

Taxonomic chimeras, artificial taxa created unintentionally by amalgamation of morphological traits belonging to different taxonomic units, can be found in 19th century to present day scientific literature. We recognise two types of such artefacts in Ostracoda. Chimera Type 1 is represented by species defined by morphological traits belonging to two (or more) different valid taxa at the rank of species. A thorough comparative analysis of carapace and limb characteristics of Fabaeformiscandona balatonica (Daday) sensu Bronshtein (1947) allows us to conclude that it is a chimera comprising F. balatonica and F. levanderi (Hirschmann), for which we provide new, expanded diagnoses. Chimera Type 2 refers to a genus defined by juvenile morphological traits that also occur in other genera. Analysis of Candoniella Schneider, 1956 shows it to be an artefact based on morphological traits belonging to juveniles of at least three genera: Pseudocandona Kaufmann, 1900, Fabaeformiscandona Krsti, 1972 and Neglecandona Krsti, 2006. Elimination of taxonomic artefacts is necessary to improve not only taxonomy but also adjacent domains of investigation like the ecology and geographical distribution of confused taxa. Considered in historical contexts, the creation and perpetuation of such accidental monstrosities may be attributed to social motivations as well as limitations of material, literature and communication.


Assuntos
Quimera , Crustáceos , Animais , Artefatos
9.
Zootaxa ; 5098(1): 1-84, 2022 Feb 09.
Artigo em Inglês | MEDLINE | ID: mdl-35391429

RESUMO

Type material of 10 genera, one subgenus, 110 species and 28 subspecies described by Wicher (1959), Krmmelbein (1961, 1962, 1963, 1964a,b, 1965a,b), Krmmelbein Weber (1971) and Bate (1972, 1994) are re-illustrated using optical digital technology in order to provide a standard reference for future systematic work and its biostratigraphical and palaeoenvironmental application. The genera are: Brasacypris, Coriacina, Hourcqia, Ilhasina, Looneyellopsis, Pattersoncypris, Petrobrasia, Reconcavona, Salvadoriella, and Tucanocypris, and subgenus Cypridea (Sebastianites).


Assuntos
Crustáceos , Animais , Brasil
10.
Zootaxa ; 5094(3): 351-395, 2022 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-35391446

RESUMO

Details of the post-embryonic development of two Notodromadidae species, Notodromas trulla Smith Kamiya, 2014 and Newnhamia fenestrata King, 1855, (subfamily Notodromadinae) are provided, and compared with previous ontogenetic studies on other podocopid families and superfamilies. The ontogenetic development is generally similar to other families, consisting of eight free-living juvenile stages and one adult stage, but the first instar, with a leg-like mandible, resembles that of the Cyprididae, rather than other families. From the A-7 instar onwards, the ventral margin of the carapace is a flattened ovoid, and the dorsolateral eye cups are separated, resembling those of the adults, suggesting that a neustonic lifestyle, similar to that of the adults, is embraced from a very early age. In addition to the ventral margin, other apomorphies of the Notodromadinae include spur-like protrusions on the walking legs of juveniles, which become reduced in adults, and features of the mandibles, probably related to neustonic feeding. Overall, Ne. fenestrata has more plesiomorphic features than No. trulla, and most differences between the two species are related to sexually selected characters, such as different sexually dimorphic features of the antennae. This suggests that sexual selection has been the main evolutionary driving force causing morphological divergence in the subfamily. The two taxa, one from Japan (No. trulla), the other Australia (Ne. fenestrata), have perhaps been separated since the breakup of Pangaea, which started in the Middle Jurassic. This suggests that despite the long geographical isolation, many aspects of ostracod anatomy have remained unchanged over long periods of time. On reviewing the taxonomy of the family, we conclude that monophyly needs to be confirmed with further work, and the subfamily Notodromadinae can be divided into two groups: the Notodromas-group and the Newnhamia-group.


Assuntos
Evolução Biológica , Crustáceos , Animais , Humanos
11.
Zootaxa ; 5094(2): 288-300, 2022 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-35391451

RESUMO

A new stomatopod-associated species of the alpheid shrimp genus Athanas Leach, 1814 is described based on several specimens collected in shallow waters of northern New Caledonia. Athanas philippei n. sp. differs from all of its congeners by a unique combination of morphological characteristics, as well as a diagnostic colour pattern. All specimens of A. philippei n. sp. were collected from burrows, including one with its host, a large tiger mantis shrimp, Lysiosquillina maculata (Fabricius, 1793) (Lysiosquillidae). Thus, the new species represents the fifth species of Athanas known or presumed to be associated with the spacious burrows of stomatopods.


Assuntos
Decápodes , Distribuição Animal , Estruturas Animais/anatomia & histologia , Animais , Crustáceos , Nova Caledônia
12.
Carbohydr Polym ; 287: 119349, 2022 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-35422296

RESUMO

Chitin is one of the most diverse and naturally occurring biopolymers, and it is mainly present in crustaceans, insects, and fungi. Chitosan is derived from chitin by deacetylation process. It is important to note that the conventional chemical method of extracting chitin includes disadvantages and it poses various environmental issues. Recently, the green extraction techniques have perceived substantial development in the field of polymer chemistry. A variety of methods have been successfully developed using green extraction techniques for extracting chitin and chitosan from various resources. It includes the use of ionic liquids (ILs), deep eutectic solvents (DES), microbial fermentation, enzyme-assisted extraction (EAE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), subcritical water extraction (SWE), and electrochemical extraction (ECE). In this review, the extraction of chitin and chitosan using greener approaches were summarized. In addition, challenges, opportunities and future perspectives of green extraction methods have also been narrated.


Assuntos
Quitosana , Líquidos Iônicos , Animais , Biopolímeros , Quitina , Crustáceos , Solventes
13.
Sci Rep ; 12(1): 4931, 2022 03 23.
Artigo em Inglês | MEDLINE | ID: mdl-35322086

RESUMO

The class Branchiopoda, whose origin dates back to Cambrian, includes ~ 1200 species which mainly occupy freshwater habitats. The phylogeny and systematics of the class have been debated for long time, until recent phylogenomic analyses allowed to better clarify the relationships among major clades. Based on these data, the clade Anostraca (fairy and brine shrimps) is sister to all other branchiopods, and the Notostraca (tadpole shrimps) results as sister group to Diplostraca, which includes Laevicaudata + Spinicaudata (clam shrimps) and Cladoceromorpha (water fleas + Cyclestherida). In the present analysis, thanks to an increased taxon sampling, a complex picture emerges. Most of the analyzed mitogenomes show the Pancrustacea gene order while in several other taxa they are found rearranged. These rearrangements, though, occur unevenly among taxa, most of them being found in Cladocera, and their taxonomic distribution does not agree with the phylogeny. Our data also seems to suggest the possibility of potentially homoplastic, alternative gene order within Daphniidae.


Assuntos
Cladóceros , Genoma Mitocondrial , Animais , Cladóceros/genética , Crustáceos , Ordem dos Genes , Rearranjo Gênico , Filogenia
14.
Artigo em Inglês | MEDLINE | ID: mdl-35302910

RESUMO

Contamination of vegan products and vegan shrimps with real shrimps and crustaceans must be avoided to comply with vegan claims, even more for preventing strong allergenic reactions. Therefore, the detection of crustaceans must be reliable and sensitive enough for authenticity, traceability, and food safety purposes. A new real-time PCR assay was developed targeting the mitochondrial 16S rRNA gene of crustaceans and was optimised to avoid critical mismatches with primers and probe. By testing several crustacean species and common food ingredients, the method was demonstrated to be specific to crustaceans only. To comply with the limit of non-vegan contamination established at 0.1% (w/w) by the European Vegetarian Union, dedicated cut-off CT values were determined on vegan raw materials and on vegan shrimps spiked with crustacean materials. The method reached a sensitivity ≤ 0.0005% (w/w), which was further confirmed on reference materials containing a similar amount of crustacean.


Assuntos
Crustáceos , Veganos , Animais , Crustáceos/genética , Primers do DNA , Humanos , RNA Ribossômico 16S/genética , Reação em Cadeia da Polimerase em Tempo Real/métodos
15.
Artigo em Inglês | MEDLINE | ID: mdl-35302928

RESUMO

In this study, we developed and validated a liquid chromatography triple quadrupole tandem mass spectrometry (LC-MS/MS) method used to simultaneously determine levels of four fluoroquinolones (ofloxacin, norfloxacin, lomefloxacin, and pefloxacin) and two antipsychotics (diazepam and methaqualone) in fish and shrimp. The samples were extracted with a mixture of anhydrous sodium sulfate and acetonitrile, and purified by C18 cartridge solid-phase extraction with an optimized eluent. The MS2 method was applied to recognize the molecular structure of these compounds according to a main fragmentation scheme. The key ions of identification and quantification were deduced from chemical structures. Multiple reaction monitoring was used to quantitatively analyse the compounds of interest. Satisfactory linearities were obtained (R2 ≥0.99) with the limits of quantitation (LOQs) ranging between 0.03 and 1.96 µg kg-1. The recoveries were 74-122%, with a relative standard deviation (RSD) below 4.9% for these compounds at the spiking level of three, five, and ten times the LODs, respectively. The LC-MS/MS method allows precise and sensitive determination of residues of six important banned veterinary drugs in fish and shrimp tissue. This methodological approach solved the problem imposed by the need for two or more analysis methods to analyse the compounds of interest described in this study.


Assuntos
Antipsicóticos , Espectrometria de Massas em Tandem , Animais , Cromatografia Líquida de Alta Pressão , Cromatografia Líquida , Crustáceos , Peixes , Fluoroquinolonas/análise , Extração em Fase Sólida
16.
Mar Drugs ; 20(3)2022 Mar 12.
Artigo em Inglês | MEDLINE | ID: mdl-35323505

RESUMO

In recent years, the food, pharma, and cosmetic industries have shown considerable interest in bioactive molecules of marine origin that show high potential for application as nutraceuticals and therapeutic agents. Astaxanthin, a lipid-soluble and orange-reddish-colored carotenoid pigment, is one of the most investigated pigments. Natural astaxanthin is mainly produced from microalgae, and it shows much stronger antioxidant properties than its synthetic counterpart. This paper aims to summarize and discuss the important aspects and recent findings associated with the possible use of crustacean byproducts as a source of astaxanthin. In the last five years of research on the crustaceans and their byproducts as a source of natural astaxanthin, there are many new findings regarding the astaxanthin content in different species and new green extraction protocols for its extraction. However, there is a lack of information on the amounts of astaxanthin currently obtained from the byproducts as well as on the cost-effectiveness of the astaxanthin production from the byproducts. Improvement in these areas would most certainly contribute to the reduction of waste and reuse in the crustacean processing industry. Successful exploitation of byproducts for recovery of this valuable compound would have both environmental and social benefits. Finally, astaxanthin's strong biological activity and prominent health benefits have been discussed in the paper.


Assuntos
Crustáceos , Resíduos , Animais , Indústria de Processamento de Alimentos , Humanos , Alimentos Marinhos , Xantofilas/farmacologia , Xantofilas/uso terapêutico
17.
Carbohydr Polym ; 286: 119281, 2022 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-35337528

RESUMO

Extraction of chitin from crustacean waste with acidic natural deep eutectic solvents (NADESs) is usually accompanied by degradation of chitin, which lowers the yield and molecular weight of product. Herein, this study proposed a eco-friendly and feasible route for effectively improving the yield and molecular weight of chitin by introducing N-acetyl-D-glucosamine into ternary NADESs. A high molecular weight chitin with molecular weight of 3.92 × 105 Da, purity of 90.2% and yield of 85.6% was obtained from crab shell. Compared with conventional acid/alkali and binary NADESs method, the maximum yield of chitin extracted by ChCl-G-FA2 was increased by 1.57 times and 1.39 times respectively. Molecular weight of chitin was 3.16 times that of acid/alkali method. Recycling performance evaluation revealed that the purity of chitin could still reach 80.4% after five cycles of NADESs. This study provided a eco-friendly utilization strategy for crustacean waste based on multifunctional NADESs.


Assuntos
Quitina , Animais , Crustáceos , Solventes
18.
J Exp Biol ; 225(6)2022 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-35224643

RESUMO

Stomatopod crustaceans, or mantis shrimps, are known for their extensive range of spectral sensitivity but relatively poor spectral discrimination. Instead of the colour-opponent mechanism of other colour vision systems, the 12 narrow-band colour channels they possess may underlie a different method of colour processing. We investigated one hypothesis in which the photoreceptors are proposed to act as individual wave-band detectors, interpreting colour as a parallel pattern of photoreceptor activation, rather than a ratiometric comparison of individual signals. This different form of colour detection has been used to explain previous behavioural tests in which low-saturation blue was not discriminated from grey, potentially because of similar activation patterns. Results here, however, indicate that the stomatopod Haptosquilla trispinosa was able to easily distinguish several colours, including blue of both high and low saturation, from greys. The animals did show a decrease in performance over time in an artificially lit environment, indicating plasticity in colour discrimination ability. This rapid plasticity, most likely the result of a change in opsin (visual pigment) expression, has now been noted in several animal lineages (both invertebrate and vertebrate) and is a factor we suggest needs attention and potential re-examination in any colour-based behavioural tests. As for stomatopods, it remains unclear why they achieve poor colour discrimination using the most comprehensive set of spectral sensitivities in the animal kingdom and also what form of colour processing they may utilise.


Assuntos
Visão de Cores , Animais , Percepção de Cores/fisiologia , Crustáceos/fisiologia , Opsinas , Células Fotorreceptoras de Invertebrados/fisiologia , Pigmentos da Retina/fisiologia
19.
Environ Toxicol Chem ; 41(5): 1311-1318, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35156233

RESUMO

Chemical herding agents are surfactant mixtures used to coalesce spilled oil and increase slick thickness to facilitate mechanical recovery or in situ burning. Only two herders are currently listed on the United States' National Oil and Hazardous Substances Pollution Contingency Plan or National Contingency Plan product schedule for potential use in spill response: the surface collecting agents Siltech OP-40™ and ThickSlick 6535™. Toxicity data for spill response agents are frequently available only for two estuarine species, mysid shrimp (Americamysis bahia) and inland silversides (Menidia beryllina), and are particularly limited for herding agents. Toxicity can vary over several orders of magnitude across product type and species, even within specific categories of spill response agents. Seven aquatic species were tested with both Siltech OP-40™ and ThickSlick 6535™ to evaluate acute herder toxicity and relative species sensitivity. The toxicity assessment included: acute tests with A. bahia and M. beryllina, the freshwater crustacean Ceriodaphina dubia, and the freshwater fish Pimephales promelas; development of the echinoderm Arbacia unctulate; and growth of a freshwater alga Raphidocelis subcapitata and marine alga Dunaliella tertiolecta. Siltech acute toxicity values ranged from 1.1 to 32.8 ppm. ThickSlick acute toxicity values ranged from 2.2 to 126.4 ppm. The results of present study show greater toxicity of Siltech compared to ThickSlick with estimated acute hazard concentrations intended to provide 95% species protection of 1.1 and 3.6 ppm, respectively, on empirical data and 0.64 and 3.3 ppm, respectively, with the addition of interspecies correlation data. The present study provides a greater understanding of species sensitivity of these two oil spill response agents. Environ Toxicol Chem 2022;41:1311-1318. © 2022 SETAC. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.


Assuntos
Poluição por Petróleo , Petróleo , Poluentes Químicos da Água , Animais , Crustáceos/fisiologia , Peixes , Poluição por Petróleo/análise , Tensoativos , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/toxicidade
20.
J Exp Biol ; 225(6)2022 03 15.
Artigo em Inglês | MEDLINE | ID: mdl-35129197

RESUMO

CRISPR/Cas9 technology has been applied to many arthropods. However, application of this technology to crustaceans remains limited because of the unique characteristics of embryos. Our group has developed a microinjection system to introduce the CRISPR/Cas9 system into Neocaridina heteropoda embryos (one-cell stage). Using the developed method, we mutated the target gene Nh-scarlet (N. heteropoda scarlet), which functions in eye development and pigmentation. The results showed that both eye color and shape were altered in individuals in which Nh-scarlet was knocked out. Furthermore, this system was also successfully applied to another decapod crustacean, Eriocheir sinensis. DNA sequencing revealed that the zoeae with red eyes had an edited version of Es-scarlet. This study provides a stable microinjection method for freshwater crustaceans, and will contribute to functional genomics studies in various decapods.


Assuntos
Sistemas CRISPR-Cas , Decápodes , Animais , Sistemas CRISPR-Cas/genética , Crustáceos , Decápodes/genética , Humanos , Microinjeções , Mutagênese
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