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1.
Rev Sci Instrum ; 95(10)2024 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-39373609

RESUMO

Determining a substance's refractive index (RI) is of great importance in a wide variety of disciplines, such as chemistry, biomedicine, engineering, and optics, to name just a few. This work utilizes critical-angle reflectometry to measure the refractive index of dentin, a biological tissue characterized by its high RI and complex structure. We fabricated a hollow semicircular prism and filled it with bromonaphthalene (1-B), allowing us to measure RIs up to 1.65. We used 5 tooth samples with exposed dentin and a laser diode beam (λ = 650 nm) incident on interface 1-B/Dentine; the reflectance curve was measured from 60° to 75°. The critical angle is determined, and dentin RI is calculated. The real part of the calculated dentine's RI coincided well with those determined by other more expensive and specialized techniques with high technical support.


Assuntos
Dentina , Refratometria , Humanos , Dentina/química , Refratometria/instrumentação
2.
J Appl Oral Sci ; 32: e20240154, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39258716

RESUMO

OBJECTIVE: Adhesion to dentin is a first step for a successful microbial root canal colonization. Cell hydrophobicity seems to have some influence in the Candida species adhesion to surfaces. To measure cell surface hydrophobicity and to investigate the adherence ability to human dentin among Candida albicans strains isolated from root canal and lingual dorsum via an in vitro study. METHODOLOGY: adhesion was quantified in function of dentin area covered by blastospores and/or hyphae presence detected by epifluorescence microscope. Cell surface hydrophobicity was estimated by assessing the percentage migration of cells from an aqueous phase to a hydrocarbon phase. Contact angles were measured by the sessile drop technique on the dentin surface using a contact angle measurements apparatus. We also examined the correlation between adhesion ability and hydrophobicity. RESULTS: although there was some intra-species variation in cell surface hydrophobicity, most isolates were characterized by moderate hydrophobicity. There was no significant difference in this parameter when the isolation niche was considered. Both root canal and lingual dorsum yeasts were able to adhere to dentin. No association was found between the strains' site of isolation and adhesion. Moreover, cell surface hydrophobicity and adhesion ability were not correlated. CONCLUSION: although hydrophobicity can influence Candida albicans virulence in many ways, this study suggests that this parameter by itself was not a good predictor of adhesion to dentin.


Assuntos
Candida albicans , Adesão Celular , Dentina , Interações Hidrofóbicas e Hidrofílicas , Propriedades de Superfície , Candida albicans/fisiologia , Humanos , Dentina/microbiologia , Adesão Celular/fisiologia , Cavidade Pulpar/microbiologia , Microscopia de Fluorescência
3.
Microb Pathog ; 196: 106965, 2024 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-39321968

RESUMO

OBJECTIVE: This work aimed to evaluate the in vitro effect of zinc oxide-eugenol paste (ZOE) on planktonic aggregates (EfPA) and biofilm (EfBio) of Enterococcus faecalis, focusing on their morphological aspects observed and analyzed using atomic force microscopy (AFM). DESIGN: The eugenol and paste were characterized by Gas Chromatography coupled with Mass Spectrometry (GC-MS) and Fourier Transform Infrared Spectroscopy (FTIR), respectively. The effect of ZOE on EfPA and EfBio was evaluated by a direct-contact test through colony counting and crystal violet staining protocol. AFM images of untreated and treated EfPA and EfBio growth on bovine dentin were obtained to analyze the morphological damage caused by the treatments. RESULTS: The characterization showed high purity in the eugenol composition and chemical interaction between the components of the paste. A bactericidal effect on aggregates was observed after 6 h of exposure, and on biofilm after 24 h of treatment (p < 0.001). A disruptive effect on the biofilm was also evident. AFM images revealed the formation of EfPA, with a notable presence of an exopolysaccharide matrix. After 6 h of ZOE treatment, there was a significant increase in the size and surface roughness profile of treated cells (p < 0.05). Loss of typical cell morphology was observed after 24 h. The effect on the biofilm showed a tendency towards a less condensed biofilm pattern in the treated group, with no differences in surface roughness. CONCLUSION: ZOE presents bactericidal action on EfPA and EfBio, promoting significant morphological changes after treatment, especially in the aggregates.


Assuntos
Biofilmes , Enterococcus faecalis , Microscopia de Força Atômica , Biofilmes/efeitos dos fármacos , Biofilmes/crescimento & desenvolvimento , Enterococcus faecalis/efeitos dos fármacos , Enterococcus faecalis/ultraestrutura , Animais , Bovinos , Espectroscopia de Infravermelho com Transformada de Fourier , Plâncton/efeitos dos fármacos , Antibacterianos/farmacologia , Cromatografia Gasosa-Espectrometria de Massas , Eugenol/farmacologia , Cimento de Óxido de Zinco e Eugenol/farmacologia , Dentina/efeitos dos fármacos , Dentina/microbiologia , Materiais Restauradores do Canal Radicular/farmacologia
4.
Clin Oral Investig ; 28(10): 550, 2024 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-39320486

RESUMO

OBJECTIVE: To evaluate the efficacy and cytotoxicity of experimental 6% and 35% hydrogen peroxide gels (HP6 or HP35) incorporated with titanium dioxide nanoparticles (NP) co-doped with nitrogen and fluorine and irradiated with a violet LED light (LT). METHODS: Bovine enamel-dentin disks adapted to artificial pulp chambers were randomly assigned to bleaching (n = 8/group): NC (negative control), NP, HP6, HP6 + LT, HP6 + NP, HP6 + NP + LT, HP35, HP35 + LT, HP35 + NP, HP35 + NP + LT, and commercial HP35 (COM). Color (ΔE00) and whiteness index (ΔWID) changes were measured before and 14 days after bleaching. The extracts (culture medium + diffused gel components) collected after the first session were applied to odontoblast-like MDPC-23 cells, which were assessed concerning their viability, oxidative stress, and morphology. The amount of HP diffused through the disks was determined. Data were analyzed by generalized linear models or Kruskal Wallis Tests (α = 5%).  RESULTS: HP6 + NP + LT exhibited ΔE00 and ΔWID higher than HP6 (p < 0.05) and similar to all HP35 groups. HP6 + NP + LT showed the lowest HP diffusion, and the highest cell viability (%) among bleached groups, preserving cell morphology and number of living cells similar to NC and NP. HP6 + LT, HP6 + NP, and HP6 + NP + LT exhibited the lowest cell oxidative stress among bleached groups (p < 0.05). HP35, HP35 + LT, and HP35 (COM) displayed the lowest cell viability. CONCLUSION: HP6 achieved significantly higher color and whiteness index changes when incorporated with nanoparticles and light-irradiated and caused lower cytotoxicity than HP35 gels. The nanoparticles significantly increased cell viability and reduced the hydrogen peroxide diffusion and oxidative stress, regardless of HP concentration. CLINICAL SIGNIFICANCE: Incorporation of co-doped titanium dioxide nanoparticles combined with violet irradiation within the HP6 gel could promote a higher perceivable and acceptable efficacy than HP6 alone, potentially reaching the optimal esthetic outcomes rendered by HP35. This approach also holds the promise of reducing cytotoxic damages and, consequently, tooth sensitivity.


Assuntos
Sobrevivência Celular , Géis , Peróxido de Hidrogênio , Nanopartículas , Titânio , Clareadores Dentários , Clareamento Dental , Peróxido de Hidrogênio/farmacologia , Peróxido de Hidrogênio/toxicidade , Clareamento Dental/métodos , Titânio/química , Titânio/toxicidade , Animais , Bovinos , Clareadores Dentários/toxicidade , Clareadores Dentários/farmacologia , Sobrevivência Celular/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Técnicas In Vitro , Odontoblastos/efeitos dos fármacos , Esmalte Dentário/efeitos dos fármacos , Distribuição Aleatória , Dentina/efeitos dos fármacos
5.
Braz Dent J ; 35: e245910, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39320002

RESUMO

This study aimed to evaluate the masking ability of different resin composite (RC) layering techniques over discolored substrates. Layering strategies were tested (n=10), using different RCs: flowable opaque, white dentin, A1 dentin, A1 body, and A1 enamel (Filtek Z350XT; 3M ESPE). Bilayer and trilayer RC combinations resulted in final thicknesses of 1 mm, 1.5 mm, and 2 mm. Substrates tested were: A1 (reference), A3, A4, B3, C2, and C4 (Filtek Z350XT Dentin; 3M ESPE). Color differences (∆E00) were measured for the RC layers over discolored substrates with the CIEDE2000 formula. The results were compared statistically (One-way ANOVA) and descriptively (acceptability=1.77 and perceptibility=0.81 thresholds). The layering strategy influenced the ∆E00 of RCs over all substrates (P<0.001). The 1 mm bilayer group combining 0.5 mm of dentin and 0.5 mm of enamel led to ∆E00 below AT for substrates A3 and B3; the 1.5 mm bilayer group combining A1 dentin (1 mm) and enamel (0.5 mm) provided ∆E00 below AT for substrates A3, A4, and C2 and ∆E00 below PT for B3; for substrate C4, the 2 mm trilayer group combining flowable opaque (0.2 mm), A1 dentin (1.3 mm) and enamel (0.5 mm) provided ∆E00 below PT, and the 1.5 mm trilayer groups (flowable opaque + 0.8 mm dentin or body + enamel) led to ∆E00 below AT. Resin Composites were effective in masking discolored substrates. The most adequate layering strategy depended on substrate shade.


Assuntos
Resinas Compostas , Resinas Compostas/química , Cor , Humanos , Teste de Materiais , Propriedades de Superfície , Esmalte Dentário , Dentina
6.
Braz Oral Res ; 38: e087, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39292126

RESUMO

This study employed e-Vol DXS cone beam computed tomography (CBCT) software to assess dentin remnants in the furcation area of mesial canals in mandibular molars during root canal retreatment (RCR). Four groups (Reciproc®, ProTaper Next®, Race Evo®, Protaper Gold®) were subjected to RCR, and CBCT images were captured before (T1) and after (T2) treatment. Measurements of remaining dentin thickness at 1 mm and 3 mm below the furcation were scrutinized. Results revealed no significant differences in mean thicknesses of mesiobuccal (MB) and mesiolingual (ML) canals at 1 mm and 3 mm from the furcation pre-treatment (T1). Post-treatment (T2) showed analogous findings, with no significant differences in mean thicknesses. However, disparities were found between MB and ML canals at both distances, both before and after retreatment. In essence, the evaluated instruments exhibited safety in RCR, implying that they are appropriate for use in critical areas of mandibular molars without inducing excessive wear. This study underscores the reliability of these instruments in navigating danger zones during RCR, and contributes valuable insights for dental practitioners who handle complex root canal scenarios in mandibular molars.


Assuntos
Tomografia Computadorizada de Feixe Cônico , Cavidade Pulpar , Dentina , Mandíbula , Dente Molar , Retratamento , Humanos , Tomografia Computadorizada de Feixe Cônico/métodos , Dente Molar/diagnóstico por imagem , Dente Molar/anatomia & histologia , Retratamento/métodos , Mandíbula/diagnóstico por imagem , Mandíbula/anatomia & histologia , Reprodutibilidade dos Testes , Cavidade Pulpar/diagnóstico por imagem , Cavidade Pulpar/anatomia & histologia , Dentina/diagnóstico por imagem , Instrumentos Odontológicos , Tratamento do Canal Radicular/métodos , Tratamento do Canal Radicular/instrumentação , Valores de Referência , Preparo de Canal Radicular/instrumentação , Preparo de Canal Radicular/métodos , Desenho de Equipamento , Estatísticas não Paramétricas
7.
J Dent ; 150: 105330, 2024 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-39214276

RESUMO

OBJECTIVES: To evaluate in vitro the effects of nano-sized sodium trimetaphosphate (TMPnano) and sodium fluoride (F) added to a 17.5 % hydrogen peroxide (H2O2) bleaching gel on the color change, enamel mechanical and morphological properties, and H2O2 transamelodentinal diffusion. MATERIALS AND METHODS: Bovine enamel/dentin discs (n = 180) were divided according to the bleaching gel: 17.5 % H2O2 (17.5 % HP); 17.5 % H2O2 + 0.1 % F (HP/F); 17.5 % H2O2 + 1 % TMPnano (HP/TMPnano); 17.5 % H2O2 + 0.1 % F + 1 % TMPnano (HP/F/TMPnano) and 35 % H2O2 (35 % HP). The gels were applied for 40 min on three sessions, each session spaced 7 days apart. The total color change (ΔE*ab) according to the Commission Internationale de l'Eclairage (CIE) L*a*b* color change measured by CIEDE2000 (ΔE00), whitening index (ΔWID), surface hardness (SH), surface roughness (Ra), cross-sectional hardness (ΔKHN), and transamelodentinal diffusion were assessed. Enamel surfaces were examined using Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray (EDS) analysis. The data were analyzed using ANOVA, followed by the Student-Newman-Keuls test (p < 0.05). RESULTS: ΔE*ab, ΔE00, and ΔWID values were comparable among the gels that produced a bleaching effect post-treatment (p < 0.001). The HP/F/TMPnano group exhibited lower mineral loss (SH and ΔKHN), Ra, and H2O2 diffusion compared to the 17.5 % HP and 35 % HP groups, which had the highest values (p < 0.001). SEM/EDS analysis revealed surface changes in all bleached groups, though these changes were less pronounced with F/TMPnano. CONCLUSIONS: The 17.5 % HP gel containing F/TMPnano maintains the bleaching effect while reducing enamel demineralization, roughness, H2O2 diffusion, and enamel morphological changes. CLINICAL RELEVANCE: Low-Concentration H2O2 bleaching gel containing F/TMPnano can be used as a novel approach to enhance safety and maintain the performance of aesthetic effects.


Assuntos
Esmalte Dentário , Géis , Dureza , Peróxido de Hidrogênio , Polifosfatos , Fluoreto de Sódio , Propriedades de Superfície , Clareadores Dentários , Clareamento Dental , Peróxido de Hidrogênio/administração & dosagem , Bovinos , Animais , Esmalte Dentário/efeitos dos fármacos , Clareadores Dentários/química , Clareadores Dentários/administração & dosagem , Clareadores Dentários/farmacologia , Polifosfatos/farmacologia , Polifosfatos/química , Polifosfatos/administração & dosagem , Clareamento Dental/métodos , Dentina/efeitos dos fármacos , Microscopia Eletrônica de Varredura , Nanopartículas/química , Difusão , Cor , Teste de Materiais , Espectrometria por Raios X
8.
Oper Dent ; 49(5): 540-550, 2024 09 01.
Artigo em Inglês | MEDLINE | ID: mdl-39135205

RESUMO

PURPOSE: This study aimed to assess the impact of battery levels in single-peak and multi-peak light-curing units (LCUs) on irradiance, and their effects on glass fiber post push-out bond strength to root dentin and the degree of conversion of dual-cure universal resin cement. METHODS AND MATERIALS: Sixty bovine roots underwent endodontic treatment and were randomly distributed into 6 groups (n=10), formed by combining two LCUs (single-peak and multipeak) and three battery levels (100%, 50%, and 10%). A spectrophotometer measured irradiance (mW/ cm2) and spectral irradiance (mW/cm2/nm). Push-out bond strength (PBS) tests occurred at three root regions (cervical, middle, and apical), with optical and scanning electron microscopy for failure mode analysis. Degree of conversion (DC) was evaluated across the root regions. Data were analyzed using two-way repeated measures ANOVA and the Tukey HSD test. The Fisher exact test verified failure modes (α=0.05). RESULTS: As multipeak LCU battery levels decreased, emitted irradiance also diminished, with notable PBS reductions in the apical thirds. Failure modes were influenced by different conditions, primarily exhibiting mixed modes. Battery levels significantly impacted DC in the multipeak LCU, particularly in the cervical region, while the single-peak LCU exhibited DC reduction at the 10% battery level in the cervical region. CONCLUSIONS: Emitted irradiance, resin cement DC, and glass fiber post bond strength to root dentin may be influenced by varying cordless LCUs and battery levels.


Assuntos
Lâmpadas de Polimerização Dentária , Vidro , Técnica para Retentor Intrarradicular , Cimentos de Resina , Cimentos de Resina/química , Bovinos , Técnica para Retentor Intrarradicular/instrumentação , Animais , Teste de Materiais , Microscopia Eletrônica de Varredura , Colagem Dentária/métodos , Análise do Estresse Dentário , Cura Luminosa de Adesivos Dentários/métodos , Técnicas In Vitro , Dentina
9.
Oper Dent ; 49(5): 564-573, 2024 09 01.
Artigo em Inglês | MEDLINE | ID: mdl-39169512

RESUMO

This study evaluated the mechanical properties of demineralized dentin matrix submitted to different bleaching treatments, as well as the changes in mass and collagen biodegradation brought about by endogenous protease. Dentin collagen matrices were prepared to receive the following treatments (n=12): no bleaching treatment (C-control), 10% carbamide peroxide (CP-Opalescence PF, Ultradent, South Jordan, UT, USA) 10%/8 hours/ day/14 days, and 40% hydrogen peroxide (HP-Opalescence Boost, Ultradent), 40 minutes per session/3 sessions. The dentin matrices were evaluated for elastic modulus and mass before and after treatments and ultimate tensile strength after treatments. The solution collected during storage was evaluated for hydroxyproline release. There was no statistically significant difference between CP and C in terms of the elastic modulus (p=0.3697) or mass variation (p=0.1333). Dentin beams treated with HP and C presented significant mass loss after the first session (p=0.0003). HP treatment led to complete degradation of collagen matrices after the second bleaching session. After the second session, CP showed higher hydroxyproline concentration than C (p<0.0001). Ultimate tensile strength was lower for CP than C (p=0.0097). CP did not affect the elastic modulus or the dentin collagen matrix mass but did promote hydroxyproline release by endogenous protease and reduce the ultimate tensile strength. HP significantly affected the mechanical properties of dentin and promoted complete degradation of the demineralized dentin collagen matrix.


Assuntos
Peróxido de Carbamida , Colágeno , Dentina , Módulo de Elasticidade , Peróxido de Hidrogênio , Resistência à Tração , Clareadores Dentários , Clareamento Dental , Dentina/metabolismo , Dentina/efeitos dos fármacos , Colágeno/metabolismo , Humanos , Clareadores Dentários/farmacologia , Peróxido de Carbamida/farmacologia , Clareamento Dental/métodos , Peptídeo Hidrolases/metabolismo , Peróxidos , Ureia/análogos & derivados , Ureia/farmacologia , Hidroxiprolina/metabolismo , Técnicas In Vitro
10.
Microbiome ; 12(1): 145, 2024 Aug 06.
Artigo em Inglês | MEDLINE | ID: mdl-39107803

RESUMO

BACKGROUND: This study aimed to engineer and optimise a dysbiotic biofilm model to develop in vitro root caries for investigating microbial modulation strategies. The model involved growing complex biofilms from a saliva inoculum collected from four volunteers using two strategies. In the first strategy ("pre-treatment strategy"), bovine root slabs were used, and two natural compounds were incorporated at time 0 of the 10-day biofilm experiment, which included sucrose cycles mimicking the cariogenic environment. In the second strategy ("post-treatment strategy"), mature biofilms were grown in a modified Calgary biofilm device coated with collagen and hydroxyapatite for 7 days and then were exposed to the same natural compounds. The metatranscriptome of each biofilm was then determined and analysed. Collagenase activity was examined, and the biofilms and dentine were imaged using confocal and scanning electron microscopy (SEM). Mineral loss and lesion formation were confirmed through micro-computed tomography (µ-CT). RESULTS: The pH confirmed the cariogenic condition. In the metatranscriptome, we achieved a biofilm compositional complexity, showing a great diversity of the metabolically active microbiome in both pre- and post-treatment strategies, including reads mapped to microorganisms other than bacteria, such as archaea and viruses. Carbohydrate esterases had increased expression in the post-treated biofilms and in samples without sugar cycles, while glucosyltransferases were highly expressed in the presence of sucrose cycles. Enrichment for functions related to nitrogen compound metabolism and organic cyclic component metabolism in groups without sucrose compared to the sucrose-treated group. Pre-treatment of the roots with cranberry reduced microbial viability and gelatinase (but not collagenase) activity (p < 0.05). SEM images showed the complexity of biofilms was maintained, with a thick extracellular polysaccharides layer. CONCLUSIONS: This root caries model was optimized to produce complex cariogenic biofilms and root caries-like lesions, and could be used to test microbial modulation in vitro. Pre-treatments before biofilm development and cariogenic challenges were more effective than post-treatments. The clinical significance lies in the potential to apply the findings to develop varnish products for post-professional tooth prophylaxis, aiming at implementing a strategy for dysbiosis reversal in translational research. Video Abstract.


Assuntos
Biofilmes , Microbiota , Cárie Radicular , Saliva , Humanos , Cárie Radicular/microbiologia , Saliva/microbiologia , Bovinos , Animais , Bactérias/genética , Bactérias/isolamento & purificação , Dentina/microbiologia , Colagenases/metabolismo
11.
J Dent ; 149: 105283, 2024 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-39096997

RESUMO

OBJECTIVE: The aim of this study was to evaluate the effect of an adhesive loaded with 0.2 % copper (Cu) and 5 % zinc oxide (ZnO) nanoparticles (Nps) on its adhesive properties and enzymatic activity at the hybrid layer ex vivo in a randomized clinical model. METHODS: Fifteen patients participated in this study, and a total of 30 third molars were used. Occlusal cavities (4 × 4 × 2 mm) were made in each tooth, and randomly divided into 2 groups: (i) Experimental group: commercial adhesive loaded with 0.2wt % CuNps and 5wt % ZnONps; and (ii) Control Group: non-loaded commercial adhesive. Teeth were restored with resin composite. Thirty days later, extractions were performed. Extracted teeth were longitudinally sectioned. Nps in powder were characterized by field emission scanning electron microscope (FE-SEM) and energy dispersive X-ray (EDX) analysis. Microtensile bond strength (µTBS), degree of conversion (DC), and nanoleakeage (NL) tests were executed. In situ zymography (Zym) was performed to evaluate the gelatinolytic activity at the hybrid layer. Student's t-test (α = 0.05) was applied for all tests. RESULTS: µTBS and DC did not show significant differences (p > 0.05) between both groups. However, NL and gelatinolytic activity at the hybrid layer showed significant values (p < 0.05) for experimental group in comparison with control group. CONCLUSION: The addition of 0.2 % CuNps and 5 % ZnONps to a universal adhesive decreases NL and gelatinolytic activity at the hybrid layer, without jeopardizing its adhesive properties. SIGNIFICANCE: This randomized clinical trial with ex vivo analysis demonstrate that a commercial adhesive modified with 0.2wt % Cu and 5wt % ZnO Nps that does not affect its adhesive properties, reducing gelatinolytic activity and nanoleakage at the hybrid layer, which should contribute to an improvement of long term bonding-dentine clinical performance.


Assuntos
Resinas Compostas , Cobre , Colagem Dentária , Microscopia Eletrônica de Varredura , Resistência à Tração , Óxido de Zinco , Humanos , Óxido de Zinco/química , Cobre/química , Colagem Dentária/métodos , Resinas Compostas/química , Nanopartículas/química , Adesivos Dentinários/química , Dentina/efeitos dos fármacos , Dentina/enzimologia , Teste de Materiais , Masculino , Cimentos de Resina/química , Adulto , Feminino , Propriedades de Superfície , Cimentos Dentários/química , Dente Serotino , Restauração Dentária Permanente/métodos , Espectrometria por Raios X
12.
J Evid Based Dent Pract ; 24(3): 101990, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-39174162

RESUMO

OBJECTIVE: The present systematic review with a network meta-analysis (NMA) aimed to evaluate the effect of high-power lasers, associated or not with fluoride compounds, to control and prevent Erosive Tooth Wear (ETW). METHODS: The review was registered in the PROSPERO (CRD42021242547) and followed the PICO question: P (population): enamel and dentin substrate; I (Intervention): high-power laser irradiation, associated or not with fluoride compounds; C (Control): no-treatment; and O (Outcomes): prevention/control of ETW. The electronic databases PubMed, Scopus, and EMBASE were searched. Two independent reviewers evaluated in vitro and in situ studies. The risk of bias was assessed using the RoBDEMAT tool. The estimated treatment effect derived from direct and indirect comparisons were analyzed and the difference between these effects was calculated based on the data of enamel and dentin surface loss (in µm). RESULTS: A total of 179 studies were retrieved and after the exclusion of duplicates, 103 studies had their titles and abstracts evaluated. Thirty-nine studies had their full text analyzed for data extraction (Cohen Kappa = 0.88). For sound enamel, the laser irradiation (L), fluoride application (F) and, the association of treatments (L + F) promoted higher protection than No-Treatment (NT). For eroded enamel, L + F and F did not differ, but both treatments reduced surface loss compared to NT and L. For sound and eroded dentin, treatments with laser increased surface loss. CONCLUSIONS: Although a high-power laser has some potential to prevent erosive tooth wear, this effect is not better than that of standard fluoride. The use of laser in the management of dentin erosive wear can be harmful.


Assuntos
Fluoretos , Erosão Dentária , Humanos , Erosão Dentária/prevenção & controle , Metanálise em Rede , Desgaste dos Dentes/prevenção & controle , Desgaste dos Dentes/etiologia , Esmalte Dentário/efeitos da radiação , Esmalte Dentário/efeitos dos fármacos , Dentina/efeitos dos fármacos , Dentina/efeitos da radiação , Terapia com Luz de Baixa Intensidade/métodos , Terapia a Laser
13.
Eur J Oral Sci ; 132(5): e13015, 2024 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-39210526

RESUMO

This study evaluated the effect of solutions containing aminomethacrylate copolymer (AA) and sodium fluoride (F; 225 ppm F-) or fluoride plus stannous chloride (FSn; 225 ppm F-, 800 ppm Sn2+) against enamel and dentin erosion/abrasion. Solutions F, FSn, AA, F+AA, FSn+AA, and deionized water as negative control were tested. Bovine enamel and dentin specimens (n = 13/solution/substrate) underwent a set of erosion-abrasion cycles (0.3% citric acid [5 min, 4×/day], human saliva [1 h, 4×/day], brushing [15 s, 2×/day], and treatments [2 min, 2×/day]) for each of five days. Initial enamel erosion was evaluated using Knoop microhardness after the first and second acid challenge on day 1, and surface loss with profilometry after day 5. KOH-soluble fluoride was assessed. Data were analyzed with ANOVA/Tukey tests. The combination of fluoride and AA resulted in higher protection against enamel erosion, whereas this was not the case for the combination of AA and FSn. All treatments protected against enamel and dentin loss. The lowest surface loss values were observed with F+AA and FSn+AA. The polymer did not significantly influence the KOH-soluble fluoride formation on enamel/dentin specimens. The aminomethacrylate copolymer effectively enhanced the efficacy of sodium fluoride against initial erosion and improved the control of enamel and dentin wear of F and FSn solutions.


Assuntos
Esmalte Dentário , Dentina , Fluoreto de Sódio , Abrasão Dentária , Erosão Dentária , Erosão Dentária/prevenção & controle , Bovinos , Esmalte Dentário/efeitos dos fármacos , Dentina/efeitos dos fármacos , Animais , Fluoreto de Sódio/uso terapêutico , Fluoreto de Sódio/farmacologia , Humanos , Abrasão Dentária/prevenção & controle , Abrasão Dentária/etiologia , Saliva/efeitos dos fármacos , Saliva/química , Fluoretos de Estanho/uso terapêutico , Cariostáticos/farmacologia , Cariostáticos/uso terapêutico , Dureza , Fluoretos/uso terapêutico , Ácido Cítrico/farmacologia , Ácido Cítrico/efeitos adversos , Escovação Dentária , Compostos de Potássio/uso terapêutico , Hidróxidos , Metacrilatos , Compostos de Estanho
14.
Braz Oral Res ; 38: e066, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39109763

RESUMO

This study assessed the physicochemical and antibiofilm properties of white mineral trioxide aggregate (MTA) associated with 1 or 2% of farnesol. Setting time was evaluated based on ISO 6876/2012. Radiopacity was evaluated by radiographic analysis. pH was assessed after time intervals of 1, 3, 7, 14, 21, and 28 days. Solubility (% mass loss) and volumetric change (by micro-CT) of the cements were evaluated after immersion in distilled water. The presence of voids inside the materials was assessed by using micro-CT. Antibiofilm activity against Enterococcus faecalis was evaluated by crystal violet assay and the modified direct contact test performed with biofilm previously formed on bovine root dentin for 14 days. Data were submitted to ANOVA/Tukey tests with 5% significance level. The incorporation of farnesol into MTA increased its setting time, but decreased its solubility at 30 days and its volumetric loss in all periods (p < 0.05). Radiopacity and solubility after 7 days were similar among the materials (p > 0.05). The association of farnesol showed the highest pH value after 1 and 3 days (p < 0.05). The association of farnesol with MTA promoted a decrease in the presence of voids, and increased the antimicrobial activity on biofilm biomass of E. faecalis (p < 0.05). In conclusion, the addition of farnesol can be suggested to improve the antimicrobial properties and the consistency of MTA.


Assuntos
Compostos de Alumínio , Biofilmes , Compostos de Cálcio , Combinação de Medicamentos , Enterococcus faecalis , Farneseno Álcool , Teste de Materiais , Óxidos , Materiais Restauradores do Canal Radicular , Silicatos , Solubilidade , Silicatos/farmacologia , Silicatos/química , Óxidos/farmacologia , Óxidos/química , Biofilmes/efeitos dos fármacos , Compostos de Cálcio/farmacologia , Compostos de Cálcio/química , Enterococcus faecalis/efeitos dos fármacos , Compostos de Alumínio/farmacologia , Compostos de Alumínio/química , Farneseno Álcool/farmacologia , Farneseno Álcool/química , Concentração de Íons de Hidrogênio , Fatores de Tempo , Bovinos , Materiais Restauradores do Canal Radicular/farmacologia , Materiais Restauradores do Canal Radicular/química , Animais , Análise de Variância , Reprodutibilidade dos Testes , Dentina/efeitos dos fármacos , Valores de Referência , Propriedades de Superfície/efeitos dos fármacos
15.
Braz Dent J ; 35: 5746, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39045986

RESUMO

This study aimed to evaluate the bond strength between relined fiberglass posts with different composite resin opacities in different thirds of the intraradicular dentin. Thirty single-rooted premolars were endodontically treated and divided into three groups (n=10): fiberglass posts relined with translucent composite resin, fiberglass posts relined with opaque composite resin, and fiberglass posts without relining. After cementation of the posts, the specimens were cut perpendicular to the long axis of the tooth in slices of approximately 1.3 mm of each third to be analyzed (cervical, middle, and apical) and then subjected to the push-out test. The bond strength (MPa) data were subjected to Shapiro-Wilk normality tests and two-way repeated measures analysis of variance, considering the experimental groups and different regions as study factors. Tukey's post-hoc test (p<.05) was applied for comparisons between the groups. In the cervical third, higher values of bond strength were found for the group relined with translucent resin, with a statistically significant difference for the other groups. In the other regions, both opaque and translucent resins showed similar results, but both showed higher values of bond strength compared to fiberglass posts without relining. The bond strength of fiberglass posts to different thirds of intraradicular dentin is influenced by composite resin relining as well as the opacity of the resin. The use of translucent composite resin is a more effective alternative for fiberglass posts relining.


Assuntos
Resinas Compostas , Colagem Dentária , Dentina , Vidro , Técnica para Retentor Intrarradicular , Humanos , Resinas Compostas/química , Colagem Dentária/métodos , Análise do Estresse Dentário , Teste de Materiais , Propriedades de Superfície , Materiais Dentários/química , Dente não Vital
16.
Braz Dent J ; 35: 5907, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39045992

RESUMO

To assess the effect of cleaning protocols on dentin contaminated with blood in reparative endodontic materials, bovine root samples were divided: no contamination (N); contamination (P); contamination and cleaning with saline (S), 2.5% NaOCl+saline (Na) or 2.5% NaOCl+17% EDTA+saline (NaE) and filled with: mineral trioxide aggregate (MTA), calcium-aluminate-cement (C), or C+collagen (Ccol) (n=13). The samples were evaluated for porosity, chemical composition, and bond strength. MTA porosity was lower than C (p=0.02) and higher than Ccol (p<0.001). P and NaE were similar (p=1.00), but higher than the other groups (p<0.001). MTA bond strength was similar to Ccol (p=0.777) and lower than C (p=0.028). P presented lower bond strength than the N (p<0.001); S and Na were similar to each other (p=0.969), but higher than P and lower than N (p<0.001). It was observed a predominance of mixed and cohesive failures. None of the samples showed Ca/P ratio values similar to human hydroxyapatite. This study showed that contamination with blood increased the materials porosity, but dentin cleaning with 2.5% NaOCl reduced this effect, and the collagen additive reduced the material porosity. Furthermore, blood contamination reduced the materials bond strength, and cleaning with saline or 2.5% NaOCl diminished this effect.


Assuntos
Sangue , Colágeno , Dentina , Porosidade , Bovinos , Dentina/efeitos dos fármacos , Colágeno/química , Animais , Raiz Dentária/química , Silicatos/química , Compostos de Cálcio/química , Colagem Dentária/métodos , Compostos de Alumínio/química , Cerâmica/química , Teste de Materiais , Materiais Biocompatíveis/química , Óxidos/química , Materiais Restauradores do Canal Radicular/química , Combinação de Medicamentos , Hipoclorito de Sódio/química
17.
Sci Rep ; 14(1): 16768, 2024 07 22.
Artigo em Inglês | MEDLINE | ID: mdl-39039132

RESUMO

This study evaluated the biocompatibility, bioactivity, porosity, and sealer/dentin interface of Sealer Plus BC (SP), Bio-C Sealer (BIOC), TotalFill BC Sealer (TF), and AH Plus (AHP). Dentin tubes filled with the sealers and empty tubes (control group) were implanted in the subcutaneous tissue of rats for different periods (n = 6 per group/period). Number of inflammatory cells (ICs), capsule thickness, von Kossa reaction, interleukin-6 (IL-6) and osteocalcin (OCN) were evaluated. Porosity and voids in the interface dentin/sealers were assessed by micro-computed tomography. The data were submitted to ANOVA/Tukey's tests (α = 0.05). Greater capsule thickness, ICs and IL-6 immunolabeling cells were observed in AHP. No significant difference in thickness of capsule, ICs, and IL-6- immunolabeling cells was detected between SP and TF, in all periods, and after 30 and 60 days between all groups. At 60 days all groups had reduction in capsule thickness, ICs and IL-6 immunolabeling cells. Von Kossa-positive and birefringent structures were observed in the capsules around the sealers. BIOC, SP, and TF exhibited OCN-immunolabeling cells. All sealers had porosity values below 5%, besides low and similar interface voids. BIOC, SP and TF are biocompatible, bioactive, and have low porosity and voids. The dentin-tube model used is an alternative for evaluating bioceramic materials.


Assuntos
Materiais Biocompatíveis , Dentina , Teste de Materiais , Animais , Porosidade , Dentina/química , Dentina/metabolismo , Materiais Biocompatíveis/química , Materiais Biocompatíveis/farmacologia , Ratos , Cerâmica/química , Interleucina-6/metabolismo , Microtomografia por Raio-X , Masculino , Ratos Wistar , Selantes de Fossas e Fissuras/química
18.
J Biomed Mater Res A ; 112(12): 2273-2288, 2024 12.
Artigo em Inglês | MEDLINE | ID: mdl-39015005

RESUMO

The objective of this study was to create injectable photo-crosslinkable biomaterials, using gelatin methacryloyl (GelMA) hydrogel, combined with a decellularized bone matrix (BMdc) and a deproteinized (BMdp) bovine bone matrix. These were intended to serve as bioactive scaffolds for dentin regeneration. The parameters for GelMA hydrogel fabrication were initially selected, followed by the incorporation of BMdc and BMdp at a 1% (w/v) ratio. Nano-hydroxyapatite (nHA) was also included as a control. A physicochemical characterization was conducted, with FTIR analysis indicating that the mineral phase was complexed with GelMA, and BMdc was chemically bonded to the amide groups of gelatin. The porous structure was preserved post-BMdc incorporation, with bone particles incorporated alongside the pores. Conversely, the mineral phase was situated inside the pore opening, affecting the degree of porosity. The mineral phase did not modify the degradability of GelMA, even under conditions of type I collagenase-mediated enzymatic challenge, allowing hydrogel injection and increased mechanical strength. Subsequently, human dental pulp cells (HDPCs) were seeded onto the hydrogels. The cells remained viable and proliferative, irrespective of the GelMA composition. All mineral phases resulted in a significant increase in alkaline phosphatase activity and mineralized matrix deposition. However, GelMA-BMdc exhibited higher cell expression values, significantly surpassing those of all other formulations. In conclusion, our results showed that GelMA-BMdc produced a porous and stable hydrogel, capable of enhancing odontoblastic differentiation and mineral deposition when in contact with HDPCs, thereby showing potential for dentin regeneration.


Assuntos
Polpa Dentária , Dentina , Gelatina , Engenharia Tecidual , Dentina/química , Engenharia Tecidual/métodos , Animais , Bovinos , Gelatina/química , Humanos , Polpa Dentária/citologia , Metacrilatos/química , Reagentes de Ligações Cruzadas/química , Hidrogéis/química , Alicerces Teciduais/química , Osso e Ossos , Células Cultivadas , Porosidade
19.
Dent Mater ; 40(10): 1584-1590, 2024 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-39068089

RESUMO

OBJECTIVES: To evaluate the effects of dentin biomodification agents (Proanthocyanidin (PAC), Cardol (CD) and Cardol-methacrylate (CDMA) on dentin hydrophilicity by contact angle measurement, viability of dental pulp stem cells (DPSCs) and nanomechanical properties of the hybrid layer (HL). METHODS: CDMA monomer was synthesized from cardol through methacrylic acid esterification. Human extracted third molars were used for all experiments. For nanomechanical tests, specimens were divided in four groups according to the primer solutions (CD, CDMA, PAC and control) were applied before adhesive and composite coating. Nanomechanical properties of the HL were analyzed by nanoindentation test using a Berkovich probe in a nanoindenter. Wettability test was performed on dentin surfaces after 1 min biomodification and measured by contact angle analysis. Cytotoxicity was assessed by a MTT assay with DPSCs after 48 and 72 h. Data were analyzed with Student's t test or Two-way ANOVA and Tukey HSD test (p < 0.05). RESULTS: CD and CDMA solutions achieved greater hydrophobicity and increased the water-surface contact angles when compared to PAC and control groups (p < 0.05). PAC group showed a greater reduction of elastic modulus in nanoindentation experiments when compared to CD and CDMA groups (p < 0.05) after 4 months of aging. CD inhibited cell proliferation compared to all further materials (p < 0.05), whilst CDMA and PAC indicated no cell cytotoxicity to human DPSCs. SIGNIFICANCE: Cardol-methacrylate provided significantly higher hydrophobicity to dentin and demonstrated remarkable potential as collagen crosslinking, attaining the lowest decrease of HL's mechanical properties. Furthermore, such monomer did not affect pulp cytotoxicity, thereby highlighting promising feasibility for clinical applications.


Assuntos
Sobrevivência Celular , Dentina , Metacrilatos , Molhabilidade , Humanos , Sobrevivência Celular/efeitos dos fármacos , Metacrilatos/química , Metacrilatos/farmacologia , Dentina/química , Proantocianidinas/farmacologia , Proantocianidinas/química , Teste de Materiais , Polpa Dentária/citologia , Dente Serotino , Células-Tronco/efeitos dos fármacos , Propriedades de Superfície , Interações Hidrofóbicas e Hidrofílicas , Técnicas In Vitro
20.
Braz Dent J ; 35: 5858, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39045991

RESUMO

ER:YAG laser and experimental resin-based dental adhesive loaded with functionalized carbonated apatite filler were used in this study to evaluate the dentin interaction in terms of penetration and occlusion of the dentinal tubules aiding in the control of dentin hypersensitivity (DH). Spheroidal Carbonated apatite nanoparticles (N-CAP), with an average size of 20±5 nm diameter, were synthesized, characterized, and incorporated in a universal adhesive "All Bond Universal, Bisco, USA", in (2% weight) concentration. Er:YAG laser "Lightwalker, FOTONA, EU" was adjusted to an energy output of 40mJ/ pulse and pulse repetition of 10 Hz for 10 seconds. Dentin specimens were prepared from the buccal surface of 75 extracted sound human molars. The specimens were randomly divided into five groups (n=15) according to the surface treatment: Group (L): Laser only; Group (LB): Laser in combination with adhesive; Group (LBN): Laser in combination with adhesive loaded with N-CAP; Group (B): adhesive only; and Group (BN): adhesive loaded with N-CAP. Depth of penetration and occlusion of the dentinal tubules were assessed using Environmental Scanning Electron Microscope Examination (ESEM). One-way ANOVA was used to compare groups, followed by a pairwise test for multiple comparisons (α=0.05). Groups (LB), and (LBN) showed the highest mean of dentinal tubules' penetration, with a non-significant difference between them. In contrast, the specimens treated with laser only (L) showed the most minor penetration. The employment of ER-YAG laser irradiation with the adhesive loaded with N-CAP was evaluated to be effective in penetrating and occluding the opened dentinal tubules.


Assuntos
Lasers de Estado Sólido , Lasers de Estado Sólido/uso terapêutico , Humanos , Apatitas/química , Dentina , Microscopia Eletrônica de Varredura , Nanopartículas/química , Sensibilidade da Dentina , Técnicas In Vitro , Cimentos Dentários/química
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