Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 7.381
Filtrar
1.
Life Sci Alliance ; 6(1)2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36384894

RESUMO

The role of the alternate G protein-coupled estrogen receptor 1 (GPER1) in colorectal cancer (CRC) development and progression is unclear, not least because of conflicting clinical and experimental evidence for pro- and anti-tumorigenic activities. Here, we show that low concentrations of the estrogenic GPER1 ligands, 17ß-estradiol, bisphenol A, and diethylstilbestrol cause the generation of lagging chromosomes in normal colon and CRC cell lines, which manifest in whole chromosomal instability and aneuploidy. Mechanistically, (xeno)estrogens triggered centrosome amplification by inducing centriole overduplication that leads to transient multipolar mitotic spindles, chromosome alignment defects, and mitotic laggards. Remarkably, we could demonstrate a significant role of estrogen-activated GPER1 in centrosome amplification and increased karyotype variability. Indeed, both gene-specific knockdown and inhibition of GPER1 effectively restored normal centrosome numbers and karyotype stability in cells exposed to 17ß-estradiol, bisphenol A, or diethylstilbestrol. Thus, our results reveal a novel link between estrogen-activated GPER1 and the induction of key CRC-prone lesions, supporting a pivotal role of the alternate estrogen receptor in colon neoplastic transformation and tumor progression.


Assuntos
Centrossomo , Estrogênios , Receptores de Estrogênio , Receptores Acoplados a Proteínas G , Humanos , Centrossomo/metabolismo , Instabilidade Cromossômica/genética , Colo , Dietilestilbestrol/farmacologia , Estradiol/farmacologia , Estrogênios/farmacologia , Receptores de Estrogênio/genética , Receptores de Estrogênio/metabolismo , Receptores Acoplados a Proteínas G/genética , Receptores Acoplados a Proteínas G/metabolismo
2.
Food Chem ; 402: 134128, 2023 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-36130434

RESUMO

The continuous intake of diethylstilbestrol (DES) residue seriously threatens the health of consumers, so the rapid and sensitive detection of DES is helpful for timely food safety warning. In this study, a photothermal-sensing microfluidic paper-based analytical chip (PT-Chip) was developed for effectively screening of DES residue in foods. Competitive inhibition immunochromatography was introduced and the composite of black phosphorus nanosheets and gold nanoparticles (BP-Au) was used as signal indicators. Then, the test area was irradiated by an 808 nm laser and the photothermal transduction signal was recorded by a thermal imager to achieve sensitive photothermal detection. The amount of DES was obtained by analyzing the temperature change curve of test area. The PT-Chip can achieve accurate quantification of DES with a detection limit of 0.1 µg/L. And the successful determination of DES in different food samples indicating that the PT-Chip can be used as a promising detection device.


Assuntos
Ouro , Nanopartículas Metálicas , Ouro/química , Microfluídica , Nanopartículas Metálicas/química , Dietilestilbestrol , Fósforo
3.
Chem Biol Interact ; 368: 110243, 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36374910

RESUMO

Many environmental pollutants act as endocrine-disrupting compounds by inhibiting human placental 3ß-hydroxysteroid dehydrogenase/Δ5-4 isomerase type 1 (HSD3B1) and aromatase (CYP19A1) activities. In this study, we screened 13 chemicals of environmental concern for their ability to inhibit human HSD3B1 and CYP19A1 by measuring the conversion of pregnenolone to progesterone for HSD3B1 activity and the conversion of testosterone to 17ß-estradiol for CYP19A1 activity in human JEG-3 choriocarcinoma cell microsomes. HSD3B1 had an apparent Km of 0.323 µM and an apparent Vmax of 0.111 nmol/mg/min and CYP19A1 had an apparent Km of 56 nM and an apparent Vmax of 0.177 nmol/mg protein/min. 17ß-Estradiol, bisphenol A, and bisphenol AF competitively inhibited HSD3B1 with Ki values of 0.8, 284.1, and 141.2 µM, respectively, while diethylstilbestrol had a mixed inhibition on human HSD3B1 with the Ki of 8.0 µM. Ketoconazole, bisphenol A, and bisphenol AF noncompetitively inhibited CYP19A1 with Ki values of 10.3, 54.4, and 45.7 µM, respectively, while diethylstilbestrol and zearalenone competitively suppressed CYP19A1 with Ki values of 63.0 and 16.6 µM, respectively. Docking analysis showed that 17ß-estradiol, diethylstilbestrol, bisphenol A, and bisphenol AF bound the steroid binding pocket facing the catalytic residues Y155 and K159 of HSD3B1, and that ketoconazole, bisphenol A, and bisphenol AF bound heme binding pocket while diethylstilbestrol and zearalenone bound the steroid binding site of CYP19A1. In conclusion, 17ß-estradiol, diethylstilbestrol, bisphenol A, and bisphenol AF are human HSD3B1 inhibitors, and ketoconazole, zearalenone, diethylstilbestrol, bisphenol A, and bisphenol AF are human CYP19A1 inhibitors.


Assuntos
Aromatase , Zearalenona , Humanos , Feminino , Gravidez , Aromatase/metabolismo , Placenta , Cetoconazol , Dietilestilbestrol/metabolismo , Linhagem Celular Tumoral , Inibidores da Aromatase/farmacologia , Inibidores da Aromatase/química , Inibidores da Aromatase/metabolismo , Complexos Multienzimáticos , Estradiol/metabolismo
4.
Int J Mol Sci ; 23(18)2022 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-36142518

RESUMO

Cyclic nucleotides (cAMP, cGMP) play a major role in normal and pathologic signaling. Beyond receptors, cyclic nucleotide phosphodiesterases; (PDEs) rapidly convert the cyclic nucleotide in its respective 5'-nucleotide to control intracellular cAMP and/or cGMP levels to maintain a normal physiological state. However, in many pathologies, dysregulations of various PDEs (PDE1-PDE11) contribute mainly to organs and tissue failures related to uncontrolled phosphorylation cascade. Among these, PDE4 represents the greatest family, since it is constituted by 4 genes with multiple variants differently distributed at tissue, cellular and subcellular levels, allowing different fine-tuned regulations. Since the 1980s, pharmaceutical companies have developed PDE4 inhibitors (PDE4-I) to overcome cardiovascular diseases. Since, they have encountered many undesired problems, (emesis), they focused their research on other PDEs. Today, increases in the knowledge of complex PDE4 regulations in various tissues and pathologies, and the evolution in drug design, resulted in a renewal of PDE4-I development. The present review describes the recent PDE4-I development targeting cardiovascular diseases, obesity, diabetes, ulcerative colitis, and Crohn's disease, malignancies, fatty liver disease, osteoporosis, depression, as well as COVID-19. Today, the direct therapeutic approach of PDE4 is extended by developing allosteric inhibitors and protein/protein interactions allowing to act on the PDE interactome.


Assuntos
COVID-19 , Doenças Cardiovasculares , Inibidores da Fosfodiesterase 4 , 3',5'-AMP Cíclico Fosfodiesterases , Doenças Cardiovasculares/tratamento farmacológico , Doenças Cardiovasculares/genética , GMP Cíclico , Nucleotídeo Cíclico Fosfodiesterase do Tipo 4 , Dietilestilbestrol/análogos & derivados , Humanos , Nucleotídeos Cíclicos , Preparações Farmacêuticas , Diester Fosfórico Hidrolases
5.
Indian J Cancer ; 59(2): 269-272, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35946187

RESUMO

Clear cell carcinoma (CCC) of the uterine cervix is a rare gynecologic cancer that accounts for 4-9% of adenocarcinoma of the uterine cervix. Two types of uterine cervical CCCs are known: A type that is associated with in utero exposure to diethylstilbestrol (DES) and idiopathic type that is unrelated to DES exposure. Due to its rare incidence, the clinical behavior and pathological characteristics of CCCs are not fully described and treatment recommendations are not standardized. Moreover, only a few cases are reported on the recurrent metastatic CCCs and the results of various treatment trials are inconsistent. We present a case of successfully treated idiopathic metastatic CCC of the uterine cervix that recurred after concurrent chemoradiotherapy.


Assuntos
Adenocarcinoma de Células Claras , Carcinoma , Neoplasias do Colo do Útero , Adenocarcinoma de Células Claras/patologia , Adenocarcinoma de Células Claras/terapia , Dietilestilbestrol/uso terapêutico , Feminino , Humanos , Recidiva Local de Neoplasia/tratamento farmacológico , Neoplasias do Colo do Útero/tratamento farmacológico , Neoplasias do Colo do Útero/epidemiologia
6.
Cancer Causes Control ; 33(8): 1121-1124, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-35767133

RESUMO

PURPOSE: Women exposed to diethylstilbestrol (DES) in utero were at elevated risk of clear-cell adenocarcinoma of the vagina and cervix (CCA) as young women. Previous research suggested that this elevated risk of CCA may persist into adulthood. We extended a published analysis to measure CCA risk as these women aged. METHODS: Standardized incidence ratios (SIR) compared CCA risk among women born from 1947 through 1971 (the DES-era) to CCA risk among the comparison group of women born prior to 1947, using registry data that covered the US population. RESULTS: Incidence rates of CCA among both cohorts increased with age. Among the DES-era birth cohort, higher rates of CCA were observed across all age groups except 55-59 years. SIR estimates had wide confidence intervals that often included the null value. CONCLUSIONS: Results are consistent with prior research and suggest an elevated risk of CCA in midlife and at older ages among women exposed in utero to DES. These results highlight unresolved issues regarding cancer risk among aging DES daughters and appropriate screening guidance. The examination of population-based cancer surveillance data may be a useful tool for monitoring trends in the incidence of other rare cancers over time among specific birth cohorts.


Assuntos
Adenocarcinoma de Células Claras , Efeitos Tardios da Exposição Pré-Natal , Neoplasias do Colo do Útero , Neoplasias Vaginais , Adenocarcinoma de Células Claras/induzido quimicamente , Adenocarcinoma de Células Claras/epidemiologia , Adulto , Colo do Útero , Dietilestilbestrol/efeitos adversos , Feminino , Humanos , Efeitos Tardios da Exposição Pré-Natal/induzido quimicamente , Efeitos Tardios da Exposição Pré-Natal/epidemiologia , Neoplasias do Colo do Útero/induzido quimicamente , Neoplasias do Colo do Útero/epidemiologia , Vagina , Neoplasias Vaginais/induzido quimicamente , Neoplasias Vaginais/diagnóstico , Neoplasias Vaginais/epidemiologia
7.
Biochem Biophys Res Commun ; 617(Pt 2): 18-24, 2022 08 30.
Artigo em Inglês | MEDLINE | ID: mdl-35689838

RESUMO

Endometriosis is known to be a gynaecological condition characterised by persistent inflammation and abnormal development of endometrial stroma and glands. Researchers require a rodent model to analyse the disease environment. Animal models are the best option for investigating the etiology and effective treatment of debilitating illnesses in women since rodents, like humans, menstruate. In order to develop the model system, diethylstilbestrol (DES) was examined for its ability to induce endometriosis in rats by investigating its effect on the estrus cycle, hormones, and key markers. The results demonstrated that animals given DES had an erratic estrus cycle and aberrant hormone levels. Histomorphology revealed the development of an endometriosis environment with degenerative epithelium and enlarged glandular cells after DES induction. The higher levels of estrogen, progesterone, and MCP-1 were shown in the endometriosis induced animals. Endometriosis-induced groups had decreased levels of HOXA10 and HOXA11 and increased levels of VEGF and COX-2. Finally, the DES demonstrated endometriosis induction efficacy, implying that it might be a viable replacement for endometriosis induction.


Assuntos
Endometriose , Animais , Dietilestilbestrol/farmacologia , Endometriose/induzido quimicamente , Endométrio , Feminino , Humanos , Progesterona , Ratos , Receptores de Progesterona
8.
Sci Total Environ ; 838(Pt 3): 156386, 2022 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-35662599

RESUMO

Endocrine disruption results from exposure to chemicals that alter the function of the endocrine system in animals. Chronic 60 days of exposure to a low dose (0.1 µM) of ioxynil (IOX) or diethylstilbestrol (DES) via food was used to determine the effects of these chemicals on the physiology of the heart and thyroid follicles in juvenile zebrafish. Immunofluorescence analysis and subsequent 3D morphometric analysis of the zebrafish heart revealed that chronic exposure to IOX induced ventricle deformation and significant volume increase (p < 0.001). DES exposure caused a change in ventricle morphology, but volume was unaffected. Alongside, it was found that DES exposure upregulated endothelial related genes (angptl1b, mhc1lia, mybpc2a, ptgir, notch1b and vwf) involved in vascular homeostasis. Both IOX and DES exposure caused a change in thyroid follicle morphology. Notably, in IOX exposed juveniles, thyroid follicle hypertrophy was observed; and in DES-exposed fish, an enlarged thyroid field was present. In summary, chronic exposure of juvenile zebrafish to IOX and DES affected the heart and the thyroid. Given that both chemicals are able to change the morphology of the thyroid it indicates that they behave as endocrine disruptive chemicals (EDCs). Heart function dynamically changes thyroid morphology, and function and hence it is likely that the observed cardiac effects of IOX and DES are the source of altered thyroid status in these fish.


Assuntos
Disruptores Endócrinos , Peixe-Zebra , Animais , Dietilestilbestrol/toxicidade , Iodobenzenos , Nitrilas , Glândula Tireoide , Peixe-Zebra/fisiologia
9.
Mikrochim Acta ; 189(6): 229, 2022 05 25.
Artigo em Inglês | MEDLINE | ID: mdl-35610519

RESUMO

An impedimetric sensing strategy was developed for sensitively determining diethylstilbestrol (DES) based on a platform of porphyrin-containing covalent-organic framework (p-COF). The p-COF was synthesized using 5,10,15,20-tetra (4-aminophenyl) porphyrin (TAPP) and 1,3,6,8-tetrakis(4-formylphenyl) pyrene (TFPy) as building blocks via condensation reaction, for which p-COF was named as TAPP-TFPy-COF. Considering the large specific surface area (302.9 m2 g-1), high porosity, rich nitrogen functionality, superior electrochemical activity, and strong bioaffinity toward DNA strands, the TAPP-TFPy-COF-based platform exhibited enhanced, non-label, and amplified electrochemical signal, large number of immobilized DES-targeted aptamer strands, and fast-response toward the analyte. Electrochemical results reveal that the TAPP-TFPy-COF-based aptasensor promoted the sensing performance for the detection of DES, resulting in an extremely low limit of detection of 0.42 fg mL-1 within a DES concentration ranging from 1 fg mL-1 to 0.1 pg mL-1, which was substantially lower than those of most reported DES sensors. Furthermore, the TAPP-TFPy-COF-based aptasensor possessed outperformed stability, high selectivity, ascendant reproducibility, and acceptable applicability in diverse environments. The recovery values for DES detection in milk, tap water, and frozen shrimp were in the range 91.80-118.50% with low relative standard deviation of 0.11-4.26%. This work provides a new sensing electrochemical approach based on COF network for DES detection and shows a deep insight into the construction of COF-based biosensors, which can be extended to be used for other target compounds.


Assuntos
Aptâmeros de Nucleotídeos , Estruturas Metalorgânicas , Porfirinas , Aptâmeros de Nucleotídeos/química , Dietilestilbestrol , Limite de Detecção , Estruturas Metalorgânicas/química , Porfirinas/química , Reprodutibilidade dos Testes
10.
Anal Bioanal Chem ; 414(17): 4897-4907, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35595839

RESUMO

In the current study, the C18-modified halloysite was fabricated via silylation reaction and subsequently used as sorbent in matrix solid-phase dispersion (MSPD) for the extraction of bisphenol A and diethylstilbestrol from human placenta, followed by high-performance liquid chromatography-tandem mass spectrometry analysis. The as-prepared sorbent was characterized by scanning electron microscopy, energy-dispersive spectrometry, Fourier transform infrared spectroscopy, X-ray diffraction, and thermo-gravimetric analysis. Varied parameters such as methanol concentration in wash solvent, pH and salt concentration in elution solvent, elution volume, and mass ratio of sample to sorbent were optimized. The adsorption capacities of bisphenol A and diethylstilbestrol on the developed C18-modified halloysite were 6.3 and 14.2 mg g-1, respectively, higher than those on the commercial C18 silica gel. Under the optimal condition, the average recoveries of bisphenol A and diethylstilbestrol by MSPD varied from 91.0 to 106.0%, and the relative standard deviations were less than 10.6% for human placenta samples. The limits of detection in the human placenta were 0.2 µg kg-1 for bisphenol A and diethylstilbestrol. The simple C18-modified halloysite-based MSPD method holds great potential for the determination of trace bisphenol A and diethylstilbestrol in the human placenta and other tissues of pregnant women with high sensitivity, accuracy, and reliability.


Assuntos
Dietilestilbestrol , Extração em Fase Sólida , Compostos Benzidrílicos , Cromatografia Líquida de Alta Pressão/métodos , Argila , Feminino , Humanos , Fenóis , Placenta , Gravidez , Reprodutibilidade dos Testes , Extração em Fase Sólida/métodos , Solventes/química
11.
J Hazard Mater ; 432: 128692, 2022 06 15.
Artigo em Inglês | MEDLINE | ID: mdl-35316640

RESUMO

Polydiacetylene (PDA) is very suited for sensitively detecting large biomolecules, and its unique chromatic properties enable visual read-out. However, application to the selective detection of small molecules remains challenging. Here, bifunctional ligands are studied to amplify the color change of PDA for biorecognition of small molecules for the smartphone-based detection of diethylstilbestrol (DES). PDA is decorated with streptavidin (PDA-SA, blue), and biotin-modified DES (bio-DES) is prepared as a bifunctional ligand to couple with PDA-SA and DES antibody. Since multiple bio-DES can bind to a single SA, then multiple SAs on PDA lead to an increased surface coverage of the vesicle. In samples without DES, PDA-SA-bio-DES-DES antibody complexes will form, leading to a color transition (blue to red); this color transition is greatly amplified by antibody-induced aggregation of the complexes. When DES is present, aggregation is inhibited due to competition for the antibody and PDA-SA-bio-DES retains its blue color. A linear relationship (0.4-1250 ng mL-1) is found between the colorimetric response and the logarithmic DES concentration, with adequate selectivity, accuracy (82.24-118.64%), and precision (below 8.24%). Finally, a paper-based DES PDA biosensor is developed with visual and smartphone-based detection limits of 10 ng mL-1 and 0.85 ng mL-1 in water, respectively.


Assuntos
Técnicas Biossensoriais , Dietilestilbestrol , Ligantes , Polímero Poliacetilênico , Smartphone
12.
Mikrochim Acta ; 189(4): 137, 2022 03 09.
Artigo em Inglês | MEDLINE | ID: mdl-35260926

RESUMO

Conventional molecularly imprinted polymers (MIP)-based electrochemical sensors are generally susceptible to the changes of personal operation, electrode surface, and solution conditions. Herein, a ratiometric strategy was employed through introducing Cu2O nanoparticles (NPs) as inner reference probe to realize the reliable detection of diethylstilbestrol (DES). MIP film was prepared by electropolymerization of 1H-pyrrole-3-carboxylicacid in the presence of DES on carbon nanotubes/cuprous oxide/titanium carbide (CNT/Cu2O NPs/Ti3C2Tx) modified electrodes. The Ti3C2Tx with accordion-like structure not only possessed good electrical conductivity, but also facilitated the immobilization of Cu2O NPs, which contributed to stabilizing the signal. CNT was introduced to further improve the sensitivity of the sensor. Under optimum conditions, the MIP/CNT/Cu2O NPs/Ti3C2Tx electrochemical sensors showed a broad linear response range of 0.01 to 70 µM, and a low detection limit of 6 nM (S/N = 3). Moreover, the sensor was applied to detect DES in real samples including lake water, milk, and pork, and the recoveries for spiked standard were 88-112%. Thus, this work provides a new way for reliable DES detection.


Assuntos
Impressão Molecular , Nanopartículas , Nanotubos de Carbono , Cobre , Dietilestilbestrol , Técnicas Eletroquímicas , Limite de Detecção , Polímeros Molecularmente Impressos , Nanopartículas/química , Nanotubos de Carbono/química , Polímeros/química , Titânio
13.
Talanta ; 241: 123184, 2022 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-35032900

RESUMO

Trace determination of antibacterial agents is crucial to minimize risks of human intoxication and in the prevention of serious environmental impacts. Herein, a simple one-pot solvothermal synthesis approach for a magnetic iron oxide embed nitrogen-doped graphene (MIO@NG) nanohybrid was fabricated without the addition of any extra reductant and its application towards ultrasensitive chloramphenicol (CAP) and diethylstilbestrol (DES) electrochemical sensor is demonstrated to screen for antibiotic residue contamination in milk samples. The prepared nanohybrid was modified on a magnetic screen-printed electrode (MSPE) to make it portable for on-site detection. The determination of two additive drugs, CAP and DES, was achieved based on the reduction current response at MIO@NG modified MSPE (MIO@NG/MSPE) to eliminate interference as far as possible. Uniform dispersed MIO nanoparticles are grown in situ on the surface of nitrogen-doped graphene sheets. The morphology of MIO@NG was confirmed by transmission electron microscopy (TEM) analysis. The chemical structure of the prepared MIO@NG was characterized by x-ray diffraction (XRD), x-ray photoemission spectroscopy (XPS), Raman spectroscopy, and extended x-ray absorption fine structure (EXAFS). Moreover, the superparamagnism property was investigated by vibrating sample magnetometry (VSM). The electrochemical properties of MIO@NG were evaluated with cyclic voltammetry (CV) and square wave voltammetry (SWV). Sensor performance was evaluated by testing the electrochemical activity of CAP and DES in the presence of interferences. The MIO@NG modified electrode presented superior electrochemical performance, including high sensitivity, high catalytic activity, ultimate sensitivity, very fast detection, selectivity, and excellent performance. The MIO@NG modified electrode demonstrated a detection limit of 10 nM for the detection of CAP and 6.5 nM for DES with satisfactory recovery in real samples.


Assuntos
Grafite , Cloranfenicol/análise , Dietilestilbestrol , Técnicas Eletroquímicas , Eletrodos , Grafite/química , Humanos , Limite de Detecção , Nanopartículas Magnéticas de Óxido de Ferro , Fenômenos Magnéticos
14.
J Mol Recognit ; 35(5): e2951, 2022 05.
Artigo em Inglês | MEDLINE | ID: mdl-34981869

RESUMO

Diethylstilbestrol (DES) is a synthetic form of oestrogen that does not easily degrade in the environment and can be harmful to human health. Herein, the mechanism of the interaction between laccase and DES was investigated by various spectroscopic means and high-performance liquid chromatography (HPLC). The results of fluorescence experiments showed that the quenching of intrinsic fluorescence of laccase by DES was due to a static quenching, forming a binding site. According to the Förster non-radiative energy transfer theory (FRET), the action distance R0 between DES and laccase was 4.708 nm, r was 5.81 nm, and the energy transfer efficiency E was 22.08%, respectively. Both UV-Vis absorption spectra and FT-IR spectra indicated changes in the conformation and surroundings of the enzyme and changed in the secondary structure of laccase. Multispectral synthesis showed that the interaction of laccase with DES caused a change in the secondary structure of laccase. The degradation experiments showed that laccase could degrade DES, and the DES content decreased with time. This study provides a new theoretical basis and experimental method for further research on the reaction mechanism of the laccase degradation of DES. It may also provide a reference basis for human biological and environmental safety evaluations.


Assuntos
Dietilestilbestrol , Lacase , Sítios de Ligação , Cromatografia , Dietilestilbestrol/química , Lacase/química , Ligação Proteica , Espectrometria de Fluorescência , Espectroscopia de Infravermelho com Transformada de Fourier
15.
Am J Epidemiol ; 191(5): 775-786, 2022 03 24.
Artigo em Inglês | MEDLINE | ID: mdl-35015807

RESUMO

Suboptimal pregnancy conditions may affect ovarian development in the fetus and be associated with early natural menopause (ENM) for offspring. A total of 106,633 premenopausal participants in Nurses' Health Study II who provided data on their own prenatal characteristics, including diethylstilbestrol (DES) exposure, maternal cigarette smoking exposure, multiplicity, prematurity, and birth weight, were followed from 1989 to 2017. Cox regression models were used to estimate hazard ratios (HRs) and 95% confidence intervals (CIs) for the associations of in utero exposures with ENM. During 1.6 million person-years of follow-up, 2,579 participants experienced ENM. In multivariable models, women with prenatal DES exposure had higher risk of ENM compared with those without it (HR = 1.33, 95% CI: 1.06, 1.67). Increased risk of ENM was observed for those with low (<5.5 pounds (<2.5 kg)) versus normal (7.0-8.4 pounds (3.2-3.8 kg)) birth weight (HR = 1.21, 95% CI: 1.01, 1.45). Decreasing risk was observed per 1-pound (0.45-kg) increase in birth weight (HR = 0.93, 95% CI: 0.90, 0.97). Prenatal smoking exposure, being part of a multiple birth, and prematurity were not associated with ENM. In this large cohort study, lower birth weight and prenatal DES exposure were associated with higher risk of ENM. Our results support a need for future research to examine in utero exposures that may affect offspring reproductive health.


Assuntos
Dietilestilbestrol , Efeitos Tardios da Exposição Pré-Natal , Peso ao Nascer , Estudos de Coortes , Dietilestilbestrol/efeitos adversos , Feminino , Humanos , Menopausa , Gravidez , Efeitos Tardios da Exposição Pré-Natal/induzido quimicamente , Efeitos Tardios da Exposição Pré-Natal/epidemiologia
16.
Curr Med Imaging ; 18(1): 82-85, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-34102982

RESUMO

BACKGROUND: Vaginal adenosis is a rare clinicopathological entity which refers to the presence of glandular endometrial tissue in the vaginal wall. CASE REPORT: A gravida 2 para 0 46-year-old woman presented to the gynecology department with symptoms of chronic pelvic pain. Maternal Diethylstilbestrol (DES) usage has been proven to be effective in the development of vaginal adenosis. It can also be idiopathic. Its sonographic or Magnetic Resonance Imaging (MRI) findings have not been described in English literature previously. CONCLUSION: Here, we report the clinical, transperineal ultrasonographic (US) and MRI findings of a pathologically proven case of vaginal adenosis.


Assuntos
Doenças Vaginais , Dietilestilbestrol , Feminino , Humanos , Imageamento por Ressonância Magnética , Metaplasia/patologia , Pessoa de Meia-Idade , Vagina/diagnóstico por imagem , Vagina/patologia , Doenças Vaginais/diagnóstico por imagem , Doenças Vaginais/patologia
17.
Sci Total Environ ; 805: 150348, 2022 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-34818759

RESUMO

A new highly sensitive, selective, and inexpensive electrochemical method has been developed for simultaneously detecting diethylstilbestrol (DES) and 17ß-estradiol (E2) in environmental samples (groundwater and lake water) using a graphite sensor modified by cerium oxide nanoparticles (CPE-CeO2 NPs). The developed sensor and the materials used in its preparation were characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD). The ab initio simulation was used to evaluate the adsorption energies between both DES and E2 with the surface of the sensor. The peak current of oxidation of both hormones showed two regions of linearity. The region of greatest sensitivity was observed for the linear range of 10 nM-100 nM. The detection and quantification limits for this concentration range were 0.8/2.6 nM and 1.3/4.3 nM for DES and E2, respectively. The analytical performance of the developed method showed high sensitivity, precision, repeatability, reproducibility, and selectivity. The CPE-CeO2 NPs sensor was successfully applied to simultaneously detect DES and E2 in real samples with recovery levels above 98%.


Assuntos
Dietilestilbestrol , Técnicas Eletroquímicas , Eletrodos , Estradiol , Limite de Detecção , Reprodutibilidade dos Testes
18.
Anal Chem ; 93(47): 15667-15676, 2021 11 30.
Artigo em Inglês | MEDLINE | ID: mdl-34787394

RESUMO

Diethylstilbestrol (DES), an endocrine disrupting chemical, has been linked to serious health problems in humans. In this work, a regenerative flexible upconversion-fluorescence biosensor was designed for the detection of DES in foodstuffs and environmental samples. Herein, amino-functionalized upconversion nanoparticles (UCNPs) were synthesized and immobilized on the surface of a flexible polydimethylsiloxane substrate, which was further modified with complementary DNA and dabcyl-labeled DES aptamer. The fluorescence resonance energy transfer (FRET) system was established for DES detection between dabcyl and UCNPs as the acceptor and donor pairs, respectively, which resulted in the quenching of the upconversion luminescence intensity. In the presence of a target, the FRET system was destroyed and upconversion fluorescence was restored due to the stronger affinity of the aptamer toward DES. The designed biosensor was also implemented in a dual-mode signal readout based on images from a smartphone and spectra from a spectrometer. Under the optimized experimental conditions, good linear relationships were achieved based on imaging (y = 53.055x + 36.175, R2 = 0.9851) and spectral data (y = 1.1582x + 1.9561, R2 = 0.9897). The designed biosensor revealed great practicability with a spiked recovery rate of 77.91-97.95% for DES detection in real environment and foodstuff samples. Furthermore, the proposed biosensor was regenerated seven times with an accuracy threshold of 80% demonstrating its durability and reusability. Thus, this biosensor is expected to be applied to point-of-care and on-site detection based on the developed portable smartphone device and android application.


Assuntos
Aptâmeros de Nucleotídeos , Técnicas Biossensoriais , Nanopartículas , Dietilestilbestrol , Transferência Ressonante de Energia de Fluorescência , Humanos , Luminescência , Smartphone
19.
Anal Chim Acta ; 1187: 339171, 2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-34753561

RESUMO

Nanozyme-mediated 3,3',5,5'-tetramethylbenzidine (TMB) - H2O2 systems have spawned the establishment of multiple colorimetric sensing platforms that are effective but sometimes subject to low sensitivity. Taking temperature as the output signal, photothermal effects lead to new strategies for sensitive detection. In this paper, a colorimetric and photothermal dual-mode immunoassay for diethylstilbestrol (DES) was constructed. It is based on the oxidation reaction of TMB catalyzed by black phosphorus-gold nanoparticle (BP/Au) nanohybrids, and the kinetics as well as catalytic mechanism of the nanohybrids were investigated in detail for the first time. Herein, the nanohybrids playcatalytic and photothermal dual roles. Moreover, the one-electron oxidation product of TMB (oxidized TMB) not only acts as chromogenic agent but also an excellent NIR laser-driven photothermal agent. The temperature (ΔT/°C) was gauged by a portable digital thermometer. Through an indirect competition strategy, a simple, sensitive, and economic immunosensor was proposed. Higher DES content in the sample correlated with less BP/Au nanohybrids conjugated to the surface of ELISA microplate, a weaker color change, and a lower temperature variation when exposed to laser irradiation. This method was applied for DES determination in real samples with gratifying recovery rates, showing great promise in food safety inspection applications.


Assuntos
Técnicas Biossensoriais , Nanopartículas Metálicas , Colorimetria , Dietilestilbestrol , Ouro , Peróxido de Hidrogênio , Imunoensaio , Peroxidase , Peroxidases , Fósforo
20.
Artigo em Inglês | MEDLINE | ID: mdl-34639263

RESUMO

BACKGROUND: Psychiatric disorders in children exposed in utero to diethylstilbestrol (DES) are still debated. We report here the impact of DES prescribed to suppress lactation on the children born after such treatment and their progeny, focusing particularly on psychiatric disorders. CASE PRESENTATION: We report here an informative family in which one or more psychiatric problems (e.g., bipolarity, suicide attempts and suicide, eating disorders) were detected in all children of second-generation (DES-exposed children; n = 9), but for II-2 who died at the age of 26 years due to rupture of a congenital brain aneurysm, and were associated with non-psychiatric disorders (particularly, endometriosis and hypospadias). In the third generation, 10 out of 19 DES-exposed grandchildren had psychiatric disorders (autism spectrum disorder, bipolar disorder, dyspraxia and learning disabilities, mood and behavioral disorders, and eating disorders), often associated with comorbidities. In the fourth generation (7 DES-exposed great-grandchildren, aged between 0 and 18 years), one child had dyspraxia and autism spectrum disorder. The first daughter of the second generation (not exposed to DES) and her children and grandchildren did not have any psychiatric symptoms or comorbidities. CONCLUSIONS: To our knowledge, the high prevalence of psychiatric disorders of various severities in two, and likely three generations, including DES-free pregnancies and DES-exposed pregnancies from the same family, has never been reported. This work strengthens the hypothesis that in utero exposure to DES contributes to the pathogenesis of psychiatric disorders. It also highlights a multigenerational, and possibly transgenerational, effect of DES in neurodevelopment and psychiatric disorders.


Assuntos
Transtorno do Espectro Autista , Hipospadia , Transtornos Mentais , Efeitos Tardios da Exposição Pré-Natal , Adolescente , Criança , Pré-Escolar , Dietilestilbestrol/toxicidade , Feminino , Humanos , Lactente , Recém-Nascido , Masculino , Transtornos Mentais/induzido quimicamente , Transtornos Mentais/epidemiologia , Gravidez , Efeitos Tardios da Exposição Pré-Natal/induzido quimicamente
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...