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1.
Microbiol Res ; 265: 127179, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36099814

RESUMO

In present research, a potent fungal strain was isolated from paper mill effluent (black liquor) in order to investigate its potential for the biodegradation of lignin. Two step strategy was used to screen most efficient fungal strain having ability to growin MSM-black liquor medium and to degrade alkali lignin.The results of initial screening indicated that the strain M-2 produced comparatively higher ligninolytic zone on MSN agar plates supplemented with black liquor (BL) and alkali ligninase compared to the other isolates.The results of 18S rRNA gene sequencing revealed that strain M-2 showed ≥ 99% sequence homology with Dipodasceus australiansis.The process for the biodegradation of lignin was optimized using Taguchi Orthogonal Array design. Under optimized conditions of pH 9, 40 °C and 4% inoculum, a maximum of 89% lignin was degraded with 41% color reduction after 8 days of incubation period by Dipodasceus australiansis M-2. The pH and temperature were found to be significant terms with the p-values of 0.002 and 0.001 respectively. The laccase activity of the Dipodascus australiensis was found to be maximum of 1.511 U/mL. The HPLC analysis of lignin biodegradation indicated sharp transformation of peaks as compared to the control. Our results suggested that the strain Dipodascus australiensis M-2 possess excellent lignin degradation and color reduction capability and can be applied in waste treatment systems for pulp and paper mill effluent. In present work we are reporting first hand information regarding biodegradation of lignin by a potent strain of Dipodascus australiensis and statistical optimization of the bioprocess.


Assuntos
Resíduos Industriais , Lignina , Ágar , Álcalis , Biodegradação Ambiental , Dipodascus , Resíduos Industriais/análise , Lacase/metabolismo , Lignina/metabolismo , Papel
3.
Biochemistry (Mosc) ; 83(5): 552-561, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-29738689

RESUMO

Mitochondria are involved in many processes in eukaryotic cells. They play a central role in energy conservation and participate in cell metabolism and signaling pathways. Mitochondria are the main source of reactive oxygen species, excessive generation of which provokes numerous pathologies and cell death. One of the most promising approaches to the attenuation of oxidative stress in mitochondria is the use of targeted (i.e., transported exclusively into mitochondria) lipophilic cationic antioxidants. These compounds offer advantages over conventional water-soluble antioxidants because they induce the so-called "mild uncoupling" and can prevent collapse of the membrane potential in low, nontoxic concentrations. A novel mitochondria-targeted antioxidant, SkQT1, was synthesized and tested within the framework of the research project guided by V. P. Skulachev. The results of these experiments were initially reported in 2013; however, one publication was not able to accommodate all the data on the SkQT1 interactions with isolated mitochondria and cells. Here, we examined comparative effects of SkQT1 and SkQ1 on rat liver mitochondria (with broader spectrum of energy parameters being studied) and yeast cells. SkQT1 was found to be less effective uncoupler, depolarizing agent, inhibitor of respiration and ATP synthesis, and "opener" of a nonspecific pore compared to SkQ1. At the same time SkQ1 exhibited higher antioxidant activity. Both SkQT1 and SkQ1 prevented oxidative stress and mitochondria fragmentation in yeast cells exposed to t-butyl hydroperoxide and promoted cell survival, with SkQT1 being more efficient than SkQ1. Together with the results presented in 2013, our data suggest that SkQT1 is the most promising mitochondria-targeted antioxidant that can be used for preventing various pathologies associated with the oxidative stress in mitochondria.


Assuntos
Antioxidantes/farmacologia , Benzoquinonas/farmacologia , Dipodascus/efeitos dos fármacos , Mitocôndrias Hepáticas/efeitos dos fármacos , Compostos Organofosforados/farmacologia , Plastoquinona/análogos & derivados , Animais , Dipodascus/citologia , Dipodascus/metabolismo , Relação Dose-Resposta a Droga , Masculino , Mitocôndrias Hepáticas/metabolismo , Oxigênio/metabolismo , Plastoquinona/farmacologia , Ratos , Ratos Wistar , Espécies Reativas de Oxigênio/metabolismo , Relação Estrutura-Atividade
4.
Rev Med Chil ; 145(8): 1067-1071, 2017 Aug.
Artigo em Espanhol | MEDLINE | ID: mdl-29189866

RESUMO

Saprochaete capitata (S. capitata) fungal sepsis is a severe condition with a clinical presentation that is similar to other yeast originated fungal sepsis. It is observed in patients with hematological malignancies such as acute myeloid leukemia and neutropenia. We report a 23 year old male presenting with cough, fever and malaise. A bone marrow biopsy led to the diagnosis of acute myeloid leukemia. During the first cycle of chemotherapy the patient presented fever: blood cultures were positive for Klebsiella pneumoniae. Despite antimicrobial treatment, fever persisted; a computed tomography showed a focal splenic lesion; a left exudative pleural effusion appeared. A Matrix Assisted Laser Desorption Ionization-Time of Flight mass spectrometry identified the presence of S. capitata. After multiple antifungal treatments and pleural cavity cleansing by means of videothoracoscopy and laparoscopic splenectomy, the infection resolved and the patient completed his chemotherapy.


Assuntos
Dipodascus/isolamento & purificação , Fungemia/cirurgia , Leucemia Mieloide Aguda/microbiologia , Antifúngicos/uso terapêutico , Drenagem/métodos , Fungemia/tratamento farmacológico , Fungemia/patologia , Humanos , Masculino , Pleurisia/microbiologia , Pleurisia/patologia , Esplenectomia/métodos , Esplenopatias/microbiologia , Esplenopatias/patologia , Esplenopatias/cirurgia , Resultado do Tratamento , Adulto Jovem
5.
Digit J Ophthalmol ; 23(3): 75-77, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-29162991

RESUMO

We describe our experience managing a case of fungal keratitis in a 71-year-old immunocompetent woman. Intracameral cultures isolated Magnusiomyces capitatus, a dimorphic commensal microbe with limited pathogenicity, in an immune competent patient. Repeated corneal cultures for bacteria and fungus were negative. Initial broad-spectrum topical fortified antibiotics were eventually escalated to include topical natamycin and voriconazole and oral voriconazole. The patient underwent emergent corneal transplantation, but recurrent infection eventually required anterior chamber washout and intracameral injection of amphotericin B. A combination of topical, systemic, intracameral administration of voriconazole, intracameral administration of amphotericin B, anterior chamber washout, and penetrating keratoplasty were required to clear the infection.


Assuntos
Dipodascus/isolamento & purificação , Infecções Oculares Fúngicas/microbiologia , Ceratite/microbiologia , Idoso , Antifúngicos/uso terapêutico , Infecções Oculares Fúngicas/diagnóstico , Infecções Oculares Fúngicas/tratamento farmacológico , Feminino , Humanos , Hospedeiro Imunocomprometido , Ceratite/diagnóstico , Ceratite/tratamento farmacológico
6.
Rev. méd. Chile ; 145(8): 1067-1071, ago. 2017. graf
Artigo em Espanhol | LILACS | ID: biblio-902586

RESUMO

Saprochaete capitata (S. capitata) fungal sepsis is a severe condition with a clinical presentation that is similar to other yeast originated fungal sepsis. It is observed in patients with hematological malignancies such as acute myeloid leukemia and neutropenia. We report a 23 year old male presenting with cough, fever and malaise. A bone marrow biopsy led to the diagnosis of acute myeloid leukemia. During the first cycle of chemotherapy the patient presented fever: blood cultures were positive for Klebsiella pneumoniae. Despite antimicrobial treatment, fever persisted; a computed tomography showed a focal splenic lesion; a left exudative pleural effusion appeared. A Matrix Assisted Laser Desorption Ionization-Time of Flight mass spectrometry identified the presence of S. capitata. After multiple antifungal treatments and pleural cavity cleansing by means of videothoracoscopy and laparoscopic splenectomy, the infection resolved and the patient completed his chemotherapy.


Assuntos
Humanos , Masculino , Adulto Jovem , Leucemia Mieloide Aguda/microbiologia , Fungemia/cirurgia , Dipodascus/isolamento & purificação , Pleurisia/microbiologia , Pleurisia/patologia , Esplenectomia/métodos , Esplenopatias/cirurgia , Esplenopatias/microbiologia , Esplenopatias/patologia , Drenagem/métodos , Resultado do Tratamento , Fungemia/patologia , Fungemia/tratamento farmacológico , Antifúngicos/uso terapêutico
7.
Adv Exp Med Biol ; 973: 81-88, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28213808

RESUMO

In August 2015, Dipodascus capitatus was isolated from two patients admitted to the neonatal intensive care unit. Nosocomial acquisition of the fungus was suspected and epidemiological studies were undertaken. The patients were simultaneously hospitalized, and the comparison of the two isolates by two independent molecular typing methods have confirmed clonal dissemination of a single strain of D. capitatus. Antimicrobial susceptibility testing was useful for identifying the appropriated antifungal therapy in micafungin. To our knowledge these are the first described cases of neonatal D. capitatus infection and also the first report of successful treatment by micafungin.


Assuntos
Infecção Hospitalar/microbiologia , Dipodascus/efeitos dos fármacos , Dipodascus/isolamento & purificação , Micoses/microbiologia , Dipodascus/genética , Dipodascus/imunologia , Feminino , Genótipo , Hospitais/estatística & dados numéricos , Humanos , Lactente , Recém-Nascido , Unidades de Terapia Intensiva Neonatal/estatística & dados numéricos , Masculino , Sicília
8.
Transpl Infect Dis ; 17(4): 588-92, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-26012493

RESUMO

We describe a 33-year-old man with relapsed acute myelogenous leukemia who developed subcutaneous nodules >6 months after allogeneic hematopoietic stem cell transplant. These nodules were caused by Blastoschizomyces capitatus. The lesions progressed after treatment with a posaconazole suspension. The lesions resolved after switching to voriconazole, which was given for 21 weeks. B. capitatus is a rare infection affecting immunocompromised patients, which responds to azoles.


Assuntos
Antifúngicos/uso terapêutico , Dermatomicoses/tratamento farmacológico , Dipodascus/isolamento & purificação , Transplante de Células-Tronco Hematopoéticas , Leucemia Mieloide Aguda/terapia , Infecções Oportunistas/tratamento farmacológico , Voriconazol/uso terapêutico , Adulto , Dermatomicoses/complicações , Dermatomicoses/diagnóstico , Humanos , Leucemia Mieloide Aguda/complicações , Masculino , Infecções Oportunistas/complicações , Infecções Oportunistas/diagnóstico
9.
Folia Microbiol (Praha) ; 59(2): 107-13, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-23913100

RESUMO

Blastoschizomyces capitatus is an uncommon, opportunistic pathogenic fungus, which causes invasive and disseminated infections. This microorganism is normally present in both environmental and normal human flora. Within a host, B. capitatus is able to grow in both unicellular yeast and multicellular filamentous growth forms. In this study, we obtained in vitro morphological conversion of B. capitatus from yeast-to-mycelial phase to investigate the presence and expression of glutathione transferase (GST) enzymes in both cell forms. A protein with GST activity using the model substrate 1-chloro-2,4-dinitrobenzene was detected in both morphologies and identified by tandem mass spectrometry as a eukaryotic elongation factor 1Bγ (eEF1Bγ) protein, a member of the GST superfamily. No significant difference in GST-specific activity and kinetic constants were observed between mycelial and yeast forms, indicating that eEF1Bγ protein did not show differential expression between the two phases.


Assuntos
Dipodascus/enzimologia , Glutationa Transferase/metabolismo , Fator 1 de Elongação de Peptídeos/metabolismo , Dinitroclorobenzeno/metabolismo , Dipodascus/citologia , Micélio/citologia , Micélio/enzimologia , Espectrometria de Massas em Tandem , Leveduras/citologia , Leveduras/enzimologia
10.
Rev Iberoam Micol ; 30(4): 248-55, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23583265

RESUMO

BACKGROUND: Saprochaete capitata (formerly known as Geotrichum capitatum and Blastoschizomyces capitatus) is a ubiquitous fungus found in soil, water, air, plants and dairy products. It colonizes the skin, and bronchial and intestinal tract of healthy people producing serious opportunistic infections in patients with haematological malignancies, especially in those with acute leukaemia. Since 1960s its presence is being increasingly recognized in this group of patients. The clinical spectrum of S. capitata disseminated infections is very similar to that produced by Candida, being easily misinterpreted. The associated high mortality and low susceptibility to fluconazole and echinocandins of S. capitata require the acknowledgement of this emergent infection so that it can be properly treated. CASE REPORT: We report 5 new cases of S. capitata disseminated infection in patients with advanced haematological malignancies observed in the haematology unit between the years 2004 and 2010, and review the state-of-the-art for diagnosis and treatment of this infection. CONCLUSIONS: Based on our experience, the prophylactic use of or the empirical antifungal treatment with fluconazole and/or echinocandins would not be adequate for oncohaematological patients in those hospitals where S. capitata infection may be highly prevalent.


Assuntos
Antifúngicos/uso terapêutico , Infecção Hospitalar/microbiologia , Dipodascus/isolamento & purificação , Fungemia/microbiologia , Leucemia/complicações , Infecções Oportunistas/microbiologia , Adulto , Idoso , Protocolos de Quimioterapia Combinada Antineoplásica/efeitos adversos , Linfoma de Burkitt/complicações , Linfoma de Burkitt/tratamento farmacológico , Infecções Relacionadas a Cateter/tratamento farmacológico , Infecções Relacionadas a Cateter/microbiologia , Infecção Hospitalar/tratamento farmacológico , Dipodascus/efeitos dos fármacos , Farmacorresistência Fúngica , Quimioterapia Combinada , Evolução Fatal , Neutropenia Febril/induzido quimicamente , Feminino , Fungemia/tratamento farmacológico , Humanos , Hospedeiro Imunocomprometido , Leucemia/tratamento farmacológico , Masculino , Pessoa de Meia-Idade , Infecções Oportunistas/tratamento farmacológico
12.
Rev Iberoam Micol ; 30(4): 261-3, 2013.
Artigo em Espanhol | MEDLINE | ID: mdl-23318163

RESUMO

BACKGROUND: The significant increase in systemic fungal infections is mainly due to the increase in immunocompromised patients. The high morbimortality of these infections, along with the high hospitalization costs they generate, makes them a problem of great importance in our hospital practice. Saprochaete capitata is a rare fungus that causes invasive infections, usually in immunocompromised patients, and for which there is still no consensus on the treatment regimen to be used. CASE REPORT: We present a case of disseminated infection by this fungus in a heavily immunosuppressed patient, who died as a result of multiple organ failure despite the life support measures taken and the wide spectrum antibiotics. CONCLUSIONS: It is vital to begin the antibiotic treatment as soon as possible, as well as the monitoring and follow-up cultures to test for fungi in neutropenic patients.


Assuntos
Antifúngicos/uso terapêutico , Dipodascus/isolamento & purificação , Fungemia/microbiologia , Insuficiência de Múltiplos Órgãos/etiologia , Adulto , Anti-Infecciosos/uso terapêutico , Bacteriemia/microbiologia , Infecções por Citomegalovirus/complicações , Diagnóstico Diferencial , Dipodascus/efeitos dos fármacos , Quimioterapia Combinada , Esofagite/virologia , Evolução Fatal , Feminino , Fungemia/tratamento farmacológico , Fungemia/etiologia , Herpes Zoster/diagnóstico , Humanos , Hospedeiro Imunocomprometido , Infecções por Klebsiella/complicações , Lúpus Eritematoso Sistêmico/complicações , Testes de Sensibilidade Microbiana , Neutropenia/complicações
13.
J Bioenerg Biomembr ; 43(6): 633-44, 2011 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-22138819

RESUMO

Tightly coupled mitochondria from Yarrowia lipolytica and Dipodascus (Endomyces) magnusii yeasts were used in this study. The two yeasts are aerobes containing the fully competent respiratory chain with three energy conservation sites. Interaction of the yeast mitochondria with prooxidants (diamide, menadione, oxaloacetate, phenylarsine oxide, hydrogen peroxide, t-butyl peroxide, and ascorbate plus Fe(2+)) was studied. The prooxidants, depending on their chemical nature, either caused uncoupling (e.g., activated state 4 respiration) or inhibited oxidation of respiratory substrates. All of the agents dissipated the membrane potential without megachannel formation (no large-scale swelling of mitochondria was observed). Except for combined application of ascorbate and Fe(2+), the prooxidant-induced decrease in the membrane potential was specifically prevented by ATP, even in the cases when classic antioxidants, e.g., N-acetylcysteine, were ineffective. No permeabilization of yeast mitochondria was observed under concerted action of prooxidants and Ca(2+), suggesting that an mPTP-like pore, if it ever occurs in yeast mitochondria, is not coupled with Ca(2+) uptake.


Assuntos
Cálcio/metabolismo , Dipodascus/metabolismo , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Mitocôndrias/metabolismo , Oxidantes/farmacologia , Yarrowia/metabolismo , Oxirredução/efeitos dos fármacos , Permeabilidade/efeitos dos fármacos
14.
J Bioenerg Biomembr ; 43(6): 623-31, 2011 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-22160850

RESUMO

In this study we used tightly-coupled mitochondria from Yarrowia lipolytica and Dipodascus (Endomyces) magnusii yeasts. The two yeast strains are good alternatives to Saccharomyces cerevisiae, being aerobes containing well structured mitochondria (thus ensuring less structural limitation to observe their appreciable swelling) and fully competent respiratory chain with three invariantly functioning energy conservation points, including Complex I, that can be involved in induction of the canonical Ca²âº/P(i)-dependent mitochondrial permeability transition (mPTP pore) with an increased open probability when electron flux increases(Fontaine et al. J Biol Chem 273: 25734­25740, 1998; Bernardi et al. FEBS J 273:2077­2099, 2006). High amplitude swelling and collapse of the membrane potential were used as parameters for demonstrating pore opening. Previously (Kovaleva et al. J Bioenerg Biomembr 41:239­249, 2009; Kovaleva et al. Biochemistry (Moscow) 75: 297­303, 2010) we have shown that mitochondria from Y.lipolytica and D. magnusii were very resistant to the Ca²âºoverload combined with varying concentrations of P(i),palmitic acid, SH-reagents, carboxyatractyloside (an inhibitor of ADP/ATP translocator), as well as depletion of intramitochondrial adenine nucleotide pools, deenergization of mitochondria, and shifting to acidic pH values in the presence of high [P(i)]. Here we subjected yeast mitochondria to other conditions known to induce an mPTP in animal and plant mitochondria, namely to Ca²âº overload under hypoxic conditions (anaerobiosis). We were unable to observe Ca²âº-induced high permeability of the inner membrane of D. magnusii and Y. lipolytica yeast mitochondria under anaerobic conditions, thus suggesting that an mPTP-like pore, if it ever occurs in yeast mitochondria, is not coupled with the Ca²âº uptake. The results provide the first demonstration of ATP-dependent energization of yeast mitochondria under conditions of anaerobiosis.


Assuntos
Cálcio/metabolismo , Permeabilidade da Membrana Celular/fisiologia , Dipodascus/fisiologia , Mitocôndrias/fisiologia , Yarrowia/fisiologia , Trifosfato de Adenosina/metabolismo , Anaerobiose , Potenciais da Membrana , Dilatação Mitocondrial
15.
Rev Soc Bras Med Trop ; 44(5): 648-50, 2011 Oct.
Artigo em Português | MEDLINE | ID: mdl-22031088

RESUMO

The infections caused by Dipodascus capitatus are rare, and the treatment is difficult. We reported a case of a patient with acute myeloid leukemia. The fungus was first isolated from hemocultures, and the phenotypic identification was based on mycological methods. The genotyping was carried out by sequencing the region D1/D2 from 26 rDNA. The susceptibility tests were assayed by Etest® and by the microdilution technique. None of the antifungal treatments employed were effective. The patient died on day 17 after the mycological diagnosis. The authors discussed the emergence of such infections as well as the difficulty regarding the diagnosis and treatment.


Assuntos
Dipodascus/isolamento & purificação , Leucemia Mieloide Aguda/microbiologia , Micoses/microbiologia , Adolescente , DNA Fúngico/análise , DNA Ribossômico/análise , Dipodascus/genética , Evolução Fatal , Feminino , Genótipo , Humanos , Testes de Sensibilidade Microbiana
16.
Rev. Soc. Bras. Med. Trop ; 44(5): 648-650, Sept.-Oct. 2011. ilus, tab
Artigo em Português | LILACS | ID: lil-602915

RESUMO

As infecções causadas por Dipodascus capitatus são raras e de difícil tratamento. Aqui se relata um caso em paciente com leucemia mielocítica aguda. O isolamento fúngico ocorreu a partir de hemocultura e a identificação fenotípica baseou-se em métodos micológicos clássicos; a identificação genotípica foi realizada através do sequenciamento da região D1/D2 do 26 rDNA. Os testes de suscetibilidade foram realizados através do Etest® e microdiluição em caldo. A antifungicoterapia foi ineficaz, registrando-se óbito da paciente no 17° dia após o diagnóstico. Os autores comparam o caso com relatos similares e discutem a emergência destas infecções bem como suas dificuldades diagnósticas e terapêuticas.


The infections caused by Dipodascus capitatus are rare, and the treatment is difficult. We reported a case of a patient with acute myeloid leukemia. The fungus was first isolated from hemocultures, and the phenotypic identification was based on mycological methods. The genotyping was carried out by sequencing the region D1/D2 from 26 rDNA. The susceptibility tests were assayed by Etest® and by the microdilution technique. None of the antifungal treatments employed were effective. The patient died on day 17 after the mycological diagnosis. The authors discussed the emergence of such infections as well as the difficulty regarding the diagnosis and treatment.


Assuntos
Adolescente , Feminino , Humanos , Dipodascus/isolamento & purificação , Leucemia Mieloide Aguda/microbiologia , Micoses/microbiologia , DNA Fúngico/análise , DNA Ribossômico/análise , Dipodascus/genética , Evolução Fatal , Genótipo , Testes de Sensibilidade Microbiana
17.
J Hosp Infect ; 78(4): 274-8, 2011 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-21658800

RESUMO

In July 2002, Blastoschizomyces capitatus was isolated from four neutropenic patients in a haematology unit. Two patients died due to disseminated infection while the other two had oropharyngeal colonisation. Nosocomial acquisition of the fungus was suspected and epidemiological and environmental studies were undertaken. To determine the potential source for the acquisition of the fungus, epidemiological relationships between the patients were investigated. We performed surveillance cultures on all patients and took environmental cultures of air, inanimate surfaces, food samples, blood products and chemotherapy drugs. No direct contact transmission between patients was found and B. capitatus was isolated only in vacuum flasks used for breakfast milk distribution. All isolates were compared by four independent molecular typing methods: pulsed-field gel electrophoresis, genomic DNA restriction endonuclease analysis, randomly amplified polymorphic DNA, and polymerase chain reaction fingerprinting using a single primer specific for one minisatellite or two microsatellite DNAs. Milk vacuum flasks and clinical strains were genetically indistinguishable by all typing techniques. Milk vacuum flasks were withdrawn from all hospital units and no further B. capitatus infection was detected. Our findings suggest that clonal dissemination of a single strain of B. capitatus from vacuum flasks used for milk distribution was responsible for this nosocomial outbreak in the haematological unit.


Assuntos
Infecção Hospitalar/epidemiologia , Dipodascus/isolamento & purificação , Surtos de Doenças , Doenças Transmitidas por Alimentos/epidemiologia , Leite/microbiologia , Micoses/epidemiologia , Animais , Infecção Hospitalar/microbiologia , Impressões Digitais de DNA , DNA Fúngico/genética , Dipodascus/classificação , Dipodascus/genética , Eletroforese em Gel de Campo Pulsado , Doenças Transmitidas por Alimentos/microbiologia , Genótipo , Hospitais , Humanos , Repetições de Microssatélites , Técnicas de Tipagem Micológica/métodos , Polimorfismo de Fragmento de Restrição , Técnica de Amplificação ao Acaso de DNA Polimórfico
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