Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 217
Filtrar
1.
Blood Adv ; 5(14): 2817-2828, 2021 07 27.
Artigo em Inglês | MEDLINE | ID: mdl-34269799

RESUMO

Chronic lymphocytic leukemia (CLL) is caused by the progressive accumulation of mature CD5+ B cells in secondary lymphoid organs. In vitro data suggest that CD4+ T lymphocytes also sustain survival and proliferation of CLL clones through CD40L/CD40 interactions. In vivo data in animal models are conflicting. To clarify this clinically relevant biological issue, we generated genetically modified Eµ-TCL1 mice lacking CD4+ T cells (TCL1+/+AB0), CD40 (TCL1+/+CD40-/-), or CD8+ T cells (TCL1+/+TAP-/-), and we monitored the appearance and progression of a disease that mimics aggressive human CLL by flow cytometry and immunohistochemical analyses. Findings were confirmed by adoptive transfer of leukemic cells into mice lacking CD4+ T cells or CD40L or mice treated with antibodies depleting CD4 T cells or blocking CD40L/CD40 interactions. CLL clones did not proliferate in mice lacking or depleted of CD4+ T cells, thus confirming that CD4+ T cells are essential for CLL development. By contrast, CD8+ T cells exerted an antitumor activity, as indicated by the accelerated disease progression in TCL1+/+TAP-/- mice. Antigen specificity of CD4+ T cells was marginal for CLL development, because CLL clones efficiently proliferated in transgenic mice whose CD4 T cells had a T-cell receptor with CLL-unrelated specificities. Leukemic clones also proliferated when transferred into wild-type mice treated with monoclonal antibodies blocking CD40 or into CD40L-/- mice, and TCL1+/+CD40-/- mice developed frank CLL. Our data demonstrate that CD8+ T cells restrain CLL progression, whereas CD4+ T cells support the growth of leukemic clones in TCL1 mice through CD40-independent and apparently noncognate mechanisms.


Assuntos
Dromaiidae , Leucemia Linfocítica Crônica de Células B , Animais , Linfócitos T CD4-Positivos , Ligante de CD40/genética , Leucemia Linfocítica Crônica de Células B/genética , Camundongos , Camundongos Transgênicos , Proteínas Proto-Oncogênicas
3.
Meat Sci ; 180: 108562, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34077892

RESUMO

The available literature lacks information on the metabolic processes taking place in emu muscles after the cessation of circulation. Hence, this study was undertaken to examine the physicochemical characteristics (pH, drip loss, WHC, TBARS, L*, a*, b*) with concomitant changes in protein expression patterns (SDS-PAGE) of femoral muscle (M. iliotibialis lateralis) that occur post mortem and during the first days (0 h, 24 h, 48 h) of its maturation in 1- and 3-year-old emus. Our results indicated that the interaction between emus age and storage time had significant impact on meat pH and all color indicators. Furthermore, we detected 24 differentially expressed protein bands, representing 22 different gene products. ClueGO pathways analysis revealed that these proteins were mainly involved in glycolysis/gluconeogenesis pathway, pyruvate metabolism and pyrophosphate hydrolysis-driven proton transmembrane transporter activity. Based on the results obtained it can be assumed that early post-mortem metabolism of emu muscle is predominantly based on the glycolysis as reflected by the relative abundance alterations of the glycogenolytic and glycolytic enzymes. Moreover, the energy supplies provided by ATP and other high-energy substances degradation is higher in the group of older emus. Our findings also highlighted the complexity of the molecular mechanisms underlying the conversion of muscle to meat.


Assuntos
Dromaiidae , Carne/análise , Proteínas Musculares/química , Músculo Esquelético/química , Fatores Etários , Animais , Cor , Glicólise , Concentração de Íons de Hidrogênio , Masculino , Mudanças Depois da Morte
4.
Biol Lett ; 17(5): 20210012, 2021 05.
Artigo em Inglês | MEDLINE | ID: mdl-34034528

RESUMO

Islands off southern Australia once harboured three subspecies of the mainland emu (Dromaius novaehollandiae), the smaller Tasmanian emu (D. n. diemenensis) and two dwarf emus, King Island emu (D. n. minor) and Kangaroo Island emu (D. n. baudinianus), which all became extinct rapidly after discovery by human settlers. Little was recorded about their life histories and only a few historical museum specimens exist, including a number of complete eggs from Tasmania and a unique egg from Kangaroo Island. Here, we present a detailed analysis of eggs of dwarf emus, including the first record of an almost complete specimen from King Island. Our results show that despite the reduction in size of all island emus, especially the King Island emu that averaged 44% smaller than mainland birds, the egg remained similar sized in linear measurements, but less in volume and mass, and seemingly had a slightly thinner eggshell. We provide possible reasons why these phenomena occurred.


Assuntos
Dromaiidae , Nanismo , Animais , Aves , Ilhas , Austrália do Sul
5.
J Mycol Med ; 31(2): 101133, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33774386

RESUMO

A total of 70 feathers samples of Emu (Dromaius novaehollandiae) were collected from 7 Emu farms situated at two districts (Raigad and Thane) of Maharashtra (India) and screened for resident keratinophilic fungi. Among them, 44 isolates were recovered and identified by evaluating characteristic macro- and micro-morphological features. Further gene products corresponding to the ITS1-5.8S-ITS2 rDNA region from all isolates were amplified and sequenced. Homology search was performed using BLAST program against non-redundant nucleotide database, and significantly matched DNA sequences deposited to the NCBI Gene Bank for reference purposes. Eight identified fungal species belongs to 7 different genera named as Aphanoascus terreus Ac_MW577456 (21.43%), Microsporum gypseum Ac_MW580920 (14.29%), Ctenomyces serratus Ac_MW577459 (10.0%), Uncinocarpus orissi Ac_MW577461 (5.17%), Aphanoascus verrucosus Ac_MW577458 (4.29%), Gymnascella dankaliensis Ac_MW577460 (2.86%), Gymnoascoideus petalosporus Ac_MW577462 (2.86%) and Arthroderma tuberculatum Ac_MW577457 (1.43%).


Assuntos
Dromaiidae/microbiologia , Plumas/microbiologia , Fungos/classificação , Fungos/genética , Queratinas/metabolismo , Animais , DNA Ribossômico/genética , Dromaiidae/anatomia & histologia , Fazendas , Fungos/isolamento & purificação , Índia , Microbiologia do Solo
6.
Sci Rep ; 11(1): 851, 2021 01 13.
Artigo em Inglês | MEDLINE | ID: mdl-33441670

RESUMO

In Australia, significant shifts in species distribution have occurred with the loss of megafauna, changes in indigenous Australian fire regime and land-use changes with European settlement. The emu, one of the last megafaunal species in Australia, has likely undergone substantial distribution changes, particularly near the east coast of Australia where urbanisation is extensive and some populations have declined. We modelled emu distribution across the continental mainland and across the Great Dividing Range region (GDR) of eastern Australia, under historical, present and future climates. We predicted shifts in emu distribution using ensemble modelling, hindcasting and forecasting distribution from current emu occurrence data. Emus have expanded their range northward into central Australia over the 6000 years modelled here. Areas west of the GDR have become more suitable since the mid-Holocene, which was unsuitable then due to high precipitation seasonality. However, the east coast of Australia has become climatically sub-optimal and will remain so for at least 50 years. The north east of NSW encompasses the range of the only listed endangered population, which now occurs at the margins of optimal climatic conditions for emus. Being at the fringe of suitable climatic conditions may put this population at higher risk of further decline from non-climatic anthropogenic disturbances e.g. depredation by introduced foxes and pigs. The limited scientific knowledge about wild emu ecology and biology currently available limits our ability to quantify these risks.


Assuntos
Migração Animal/fisiologia , Demografia/tendências , Dromaiidae/fisiologia , Algoritmos , Animais , Austrália , Mudança Climática , Demografia/métodos , Ecossistema , Modelos Teóricos
7.
Gene ; 769: 145238, 2021 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-33068676

RESUMO

The emu is a useful and new breed of poultry, but their genetic improvement has not advanced yet due to their very recent domestication. Pedigree information is difficult to record because of their complex reproduction system (polyandry). To identify parent-offspring relationships in the emu, parentage test based on polymorphic DNA markers have to be developed. In this study, we isolated more than 25,000 microsatellite (simple sequence repeat, SSR) regions from Next-generation sequencing data via the QDD pipeline and developed 49 SSR markers with polymorphism in the Japanese farmed emu. The dinucleotide motifs, (AC)n, (AT)n and (AG)n, were the most frequently detected and were found on 10,167 (38.55%), 8,114 (30.76%) and 4,796 (18.18%) contigs, respectively. Forty-nine novel SSR markers were characterized in 20 individuals and showed NA ranged from 2 to 12, with an average of 4.2. HE/HO ranged from 0.389/0.071 to 0.702/1.000 with an average of 0.601/0.515. PIC value ranged from 0.059 to 0.886 with an average of 0.528, and 17 of 49 markers showed a higher polymorphism than 0.500. Thirty-four individuals were genotyped using 12 markers, and CERVUS simulations based on genotype showed that parents of all offspring were identified with 0.9995-1.0 probability. Thus, 49 novel SSR markers and a robust method for parentage test for the Japanese emu were developed.


Assuntos
Dromaiidae/genética , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Repetições de Microssatélites/genética , Animais , Feminino , Masculino , Linhagem , Polimorfismo Genético , Sequências Repetitivas de Ácido Nucleico
8.
J Vet Pharmacol Ther ; 44(4): 603-618, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33141479

RESUMO

Meloxicam is a widely used nonsteroidal anti-inflammatory drug in avian species. However, variability in pharmacokinetic (PK) and pharmacodynamic (PD) parameters in birds warrants species-specific studies for dose and dosing interval optimization. We performed a perioperative PK study of meloxicam (0.5 mg/kg, intravenously) on emus of three different age groups: 3 chicks (5 weeks old, 3.5 kg), 4 juveniles (26 weeks old, 18.8 kg) and 6 adults (66 weeks old, 38.8 kg). A two-compartment population PK model including weight as a significant covariate on clearance and central volume of distribution (V1) best fitted the data. The typical values (20 kg bird) for clearance and V1 were 0.54 L/kg/h and 0.095 L/kg. Both parameters significantly decreased with increasing weight/age. Meloxicam potency and selectivity for COX-1 and COX-2 were measured in whole blood assays (TxB2 production endpoint). Meloxicam was partially selective in emus (IC50 COX-1:COX-2 = 9.1:1). At the current empirical dose (0.5 mg/kg/24 hr), plasma meloxicam concentration is above IC50 of COX-2 for only 2 hr. PK/PD predicted dose required for 80% COX-2 inhibition over 24 hr were 3.4, 1.4 and 0.95 L/kg/day in chicks, juveniles and adult emus, respectively. The safety, therapeutic efficacy and practicality of modifying the daily dose or dose interval should be considered for dose recommendations in emus.


Assuntos
Dromaiidae , Animais , Anti-Inflamatórios não Esteroides/farmacologia , Galinhas , Ciclo-Oxigenase 2 , Meloxicam
9.
Sci Rep ; 10(1): 16541, 2020 10 06.
Artigo em Inglês | MEDLINE | ID: mdl-33024210

RESUMO

The present study reports a natural infection of emus, Dromaius novaehollandiae, by the nematode Procyrnea uncinipenis. Five adult emus from a scientific breeding farm at North Fluminense State University located in the city of Campos dos Goytacazes, Rio de Janeiro state, Brazil were necropsied, and their gastrointestinal tract were collected and examined for the presence of parasites from October 2013 to November 2015. Two of the five (40%) emus necropsied were infected with nematodes, and a portion of the nematodes were processed for light microscopy. In addition, two other nematodes (a male and a female) were prepared for scanning electron microscopy. In a female bird, one nematode was collected in the proventriculus and two nematodes in the gizzard and in the male bird four nematodes were collected in the gizzard. The morphological and morphometric analyzes allowed to identify the nematodes as P. uncinipenis, this being the first report of an infection by P. uncinipenis in emus. Therefore, we infer that these emus were naturally infected by nematodes that were considered specific to rheas.


Assuntos
Doenças das Aves/parasitologia , Dromaiidae/parasitologia , Trato Gastrointestinal/parasitologia , Nematoides/isolamento & purificação , Infecções por Nematoides/veterinária , Animais , Doenças das Aves/epidemiologia , Brasil/epidemiologia , Feminino , Moela das Aves/parasitologia , Masculino , Nematoides/anatomia & histologia , Infecções por Nematoides/epidemiologia , Nova Zelândia
10.
Mater Sci Eng C Mater Biol Appl ; 117: 111234, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32919621

RESUMO

It is very well known that bone is a hierarchically organized material produced by bone cells residing in the fluid environments filling (larger) vascular pores and (smaller) lacunar pores. The extracellular space consists of hydroxyapatite crystals, collagen type I molecules, and water with non-collageneous organics. It is less known to which extent the associated quantities (mineral, organic, and water concentrations; vascular, lacunar, and extracellular porosities) vary across species, organs, and ages. We here investigate the aforementioned quantities across femoral shaft tissues from cow, horse, emu, frog, ostrich, pig, and rabbit; by means of light microscopy and dehydration-demineralization tests; thereby revealing interesting invariances: The extracellular volume fractions of organic matter turn out to be similar across all tested non-amphibian tissues; as do the extracellular volume fractions of hydroxyapatite across all tested mammals. Hence, the chemical composition of the femoral extracellular bone matrix is remarkably "invariant" across differently aged mammals; while the water content shows significant variations, as does the partitions of water between the different pore spaces. The latter exhibit strikingly varying morphologies as well. This finding adds to the ample "universal patterns" in the sense of evolutionary developmental biology; and it provides interesting design requirements for the development of novel biomimetic tissue engineering solutions.


Assuntos
Osso e Ossos , Dromaiidae , Animais , Bovinos , Durapatita , Feminino , Cavalos , Osteócitos , Porosidade , Coelhos , Suínos
11.
Int J Dev Neurosci ; 80(7): 613-635, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32767787

RESUMO

In the current study, we examined adult neurogenesis throughout the brain of the common ostrich (Struthio camelus) and emu (Dromaius novaehollandiae) using immunohistochemistry for the endogenous markers PCNA which labels proliferating cells, and DCX, which stains immature and migrating neurons. The distribution of PCNA and DCX labelled cells was widespread throughout the brain of both species. The highest density of cells immunoreactive to both markers was observed in the olfactory bulbs and the telencephalon, especially the subventricular zone of the lateral ventricle. Proliferative hot spots, identified with strong PCNA and DCX immunolabelling, were identified in the dorsal and ventral poles of the rostral aspects of the lateral ventricles. The density of PCNA immunoreactive cells was less in the telencephalon of the emu compared to the common ostrich. Substantial numbers of PCNA immunoreactive cells were observed in the diencephalon and brainstem, but DCX immunoreactivity was weaker in these regions, preferentially staining axons and dendrites over cell bodies, except in the medial regions of the hypothalamus where distinct DCX immunoreactive cells and fibres were observed. PCNA and DCX immunoreactive cells were readily observed in moderate density in the cortical layers of the cerebellum of both species. The distribution of putative proliferating cells and immature neurons in the brain of the common ostrich and the emu is widespread, far more so than in mammals, and compares with the neognathous birds, and suggests that brain plasticity and neuronal turnover is an important aspect of cognitive brain functions in these birds.


Assuntos
Proliferação de Células/fisiologia , Hipotálamo/fisiologia , Ventrículos Laterais/fisiologia , Neurogênese/fisiologia , Neurônios/fisiologia , Animais , Dromaiidae , Células-Tronco Neurais/fisiologia , Reiformes
12.
Sci Rep ; 10(1): 11082, 2020 07 06.
Artigo em Inglês | MEDLINE | ID: mdl-32632331

RESUMO

Studies were carried out to determine the influence of age and sex on two types of fat (back fat and abdominal fat) in the emu, as these are factors that influence the composition of animal tissues. The material involved 26 emus at the age 1 (6 males), 3 (6 males) and 15 years (8 females and 6 males), kept on the same farm and fed the feed of the same nutritional value. The basic chemical composition, cholesterol and mineral content, as well as fatty acid profile of back and abdominal fat of emu were determined. Abdominal fat was characterized by higher content of fat and ash, as well as Mn and Ba. Back fat, on the other hand, showed a higher level of protein, cholesterol, C16:1 and the elements K, P, Si, Na, Ca, Mg, Fe, Zn, Se and Cu. With age, regardless of the type of fat tissue, fat content decreased and water content increased. The highest content of protein, ash, cholesterol, some fatty acids (C18:0, C18:1n9c, C18:2n6c), generally higher content of MUFA, PUFA and the elements K, P, Ca, Mg, Fe, Zn, Pb, Se, Cr, Cd, were found in the fatty tissue of 15-year-old emus. Sex did influence the content of Si, Ca, Cu, Sr, which was higher in the fatty tissue of males. The composition of emu storage fat is determined by factors such as age, sex and the location of the fat tissue in the body.


Assuntos
Tecido Adiposo/metabolismo , Colesterol/metabolismo , Dromaiidae/fisiologia , Ácidos Graxos/metabolismo , Minerais/metabolismo , Envelhecimento , Animais , Feminino , Masculino , Fatores Sexuais
13.
Seizure ; 80: 96-99, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32554293

RESUMO

PURPOSE: Complete visual review of prolonged video-EEG recordings at an EMU (Epilepsy Monitoring Unit) is time consuming and can cause problems in times of paucity of educated personnel. In this study we aimed to show non inferiority for electroclinical diagnosis using sampled review in combination with EEG analysis softreferware (P13 software, Persyst Corporation), in comparison to complete visual review. METHOD: Fifty prolonged video-EEG recordings in adults were prospectively evaluated using sampled visual EEG review in combination with automated detection software of the complete EEG record. Visually assessed samples consisted of one hour during wakefulness, one hour during sleep, half an hour of wakefulness after wake-up and all clinical events marked by the individual and/or nurses. The final electro-clinical diagnosis of this new review approach was compared with the electro-clinical diagnosis after complete visual review as presently used. RESULTS: The electro-clinical diagnosis based on sampled visual review combined with automated detection software did not differ from the diagnosis based on complete visual review. Furthermore, the detection software was able to detect all records containing epileptiform abnormalities and epileptic seizures. CONCLUSION: Sampled visual review in combination with automated detection using Persyst 13 is non-inferior to complete visual review for electroclinical diagnosis of prolonged video-EEG at an EMU setting, which makes this approach promising.


Assuntos
Dromaiidae , Epilepsia , Adulto , Animais , Eletroencefalografia , Epilepsia/diagnóstico , Humanos , Convulsões , Software
14.
Nat Commun ; 11(1): 2250, 2020 05 18.
Artigo em Inglês | MEDLINE | ID: mdl-32418985

RESUMO

Explanations for the Upper Pleistocene extinction of megafauna from Sahul (Australia and New Guinea) remain unresolved. Extinction hypotheses have advanced climate or human-driven scenarios, in spite of over three quarters of Sahul lacking reliable biogeographic or chronologic data. Here we present new megafauna from north-eastern Australia that suffered extinction sometime after 40,100 (±1700) years ago. Megafauna fossils preserved alongside leaves, seeds, pollen and insects, indicate a sclerophyllous forest with heathy understorey that was home to aquatic and terrestrial carnivorous reptiles and megaherbivores, including the world's largest kangaroo. Megafauna species diversity is greater compared to southern sites of similar age, which is contrary to expectations if extinctions followed proposed migration routes for people across Sahul. Our results do not support rapid or synchronous human-mediated continental-wide extinction, or the proposed timing of peak extinction events. Instead, megafauna extinctions coincide with regionally staggered spatio-temporal deterioration in hydroclimate coupled with sustained environmental change.


Assuntos
Mudança Climática/história , Extinção Biológica , Fósseis , Animais , Austrália , Carnivoridade , Classificação , Clima , Dromaiidae , Ecossistema , Florestas , História Antiga , Humanos , Macropodidae , Marsupiais , Nova Guiné , Paleontologia , Datação Radiométrica , Répteis , Urânio
15.
J Vet Med Sci ; 82(6): 731-734, 2020 Jun 16.
Artigo em Inglês | MEDLINE | ID: mdl-32249252

RESUMO

The emu (Dromaius novaehollandiae) is a new poultry. In this study, we investigated the haplotype composition of mitochondrial DNA among emu populations farmed in Japan. We sequenced the D-loop region in 109 individuals, and detected four substitution sites and three haplotypes (Hap-a, -b, and -c). Hap-a was the most frequently observed haplotype in the Japanese populations. Although Hap-c was a rare haplotype in not only Japanese but also Australian populations, it was detected with high frequency in the Japanese farmed population. The AMOVA indicated that 9% of total variance was "among population". The FST value was 0.087 and genetic differentiation was significant (P<0.01). These results may contribute to conserving the genetic resources available for the Japanese emu industry.


Assuntos
DNA Mitocondrial , Dromaiidae/genética , Variação Genética , Animais , Pesqueiros , Japão , Reação em Cadeia da Polimerase
16.
Mol Biol Rep ; 47(4): 2521-2527, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32086719

RESUMO

The emu (Dromaius novaehollandiae) is a useful poultry animal farmed for fat, meat, and eggs. Genetic structure and relationships among farmed emu populations in Japan are unknown and the number of microsatellite markers for genetic analysis of the emu is insufficient. In this study, we isolated 16 microsatellites from the emu genome and developed ten new microsatellite markers. These microsatellite markers were used to characterize three farm emu populations in Japan. The number of alleles ranged from 3 to 13 and the expected (HE) and observed heterozygosity (HO) of these microsatellite loci was 0.187-0.802 and 0.179-0.647, respectively. The polymorphic information content ranged from 0.176 to 0.786. Positive inbreeding coefficient (FIS) values were detected in all tested populations, and they ranged from 0.027 to 0.540. These results suggest that farm populations of the emu in Japan resulted from inbreeding. The fixation index (FST) values ranged from 0.026 to 0.061, and phylogenetic trees and population structure analysis confirmed no definitive genetic differentiation among the three populations. Therefore, these populations are at a relatively low level of genetic differentiation at present. The microsatellite markers developed in our study can be utilized for genetic analysis and preservation of genetic resources in the emu.


Assuntos
Dromaiidae/genética , Variação Genética/genética , Repetições de Microssatélites/genética , Alelos , Animais , Cruzamento/métodos , Fazendas , Feminino , Heterozigoto , Japão , Masculino , Filogenia , Polimorfismo Genético , Aves Domésticas/genética
17.
Braz. j. biol ; 80(1): 66-72, Feb. 2020. graf
Artigo em Inglês | LILACS | ID: biblio-1089274

RESUMO

Abstract Emus are large flightless birds in the ratite group and are native to Australia. Since the mid-1980s, there has been increased interest in the captive breeding of emus for the production of leather, meat and oil. The aim of this study was to identify gastrointestinal parasites in the feces of emus Dromaius novaehollandiae from a South American scientific breeding. Fecal samples collected from 13 birds were examined by direct smears, both with and without centrifugation, as well as by the fecal flotation technique using Sheather's sugar solution. Trophozoites, cysts and oocysts of protozoa and nematode eggs were morphologically and morphometrically evaluated. Molecular analysis using PCR assays with specific primers for the genera Entamoeba, Giardia and Cryptosporidium were performed. Trophozoites and cysts of Entamoeba spp. and Giardia spp., oocysts of Eimeria spp. and Isospora dromaii, as well as eggs belonging to the Ascaridida order were found in the feces. Three animals were diagnosed with Giardia spp., and three were positive for Entamoeba spp. based on PCR techniques. After analyzing the data, we concluded that emus were infected enzootically by nematode and protozoan species.


Resumo Emus são aves grandes que não voam pertencentes ao grupo das ratitas e são originários da Austrália. Desde meados da década de 1980, aumentou o interesse pela criação de emus em cativeiro para a produção de couro, carne e óleo. O objetivo deste estudo foi identificar parasitas gastrointestinais nas fezes de emus Dromaius novaehollandiae de um criatório científico da América do Sul. Amostras de fezes coletadas de 13 aves foram examinadas por esfregaços diretos, tanto com e sem centrifugação, quanto com a técnica de flutuação fecal utilizando solução de açúcar de Sheather. Trofozoítos, cistos e oocistos de protozoários e ovos de nematóides foram avaliados morfologicamente e morfometricamente. Foram realizadas análises moleculares utilizando ensaios de PCR com primers específicos para os gêneros Entamoeba, Giardia e Cryptosporidium. Trofozoítos e cistos de Entamoeba spp. e Giardia spp., oocistos de Eimeria spp. e Isospora dromaii, bem como ovos pertencentes à ordem Ascaridida foram encontrados nas fezes. Três animais foram diagnosticados com Giardia spp., e três foram positivos para Entamoeba spp. com base em técnicas de PCR. Depois de analisar os dados, concluímos que os emus estavam infectados enzooticamente por espécies de nematóides e protozoários.


Assuntos
Animais , Dromaiidae , Criptosporidiose , Cryptosporidium , Parasitos , Brasil , Fezes
18.
Blood Adv ; 4(1): 28-39, 2020 01 14.
Artigo em Inglês | MEDLINE | ID: mdl-31899800

RESUMO

Numerous B-cell lymphomas feature translocations linking oncogenes to different locations in the immunoglobulin heavy chain (IgH) locus. During Burkitt lymphoma (BL), IgH breakpoints for c-myc translocation stand either close to JH segments or within switch regions. Transcription, accessibility, and remodeling of the IgH locus are under the control of the 2 potent cis-acting enhancer elements: Eµ and the 3' regulatory region (3'RR). To ensure their respective contributions to oncogene deregulation in the context of the endogenous IgH locus, we studied transgenic mice harboring a knock-in of c-myc in various positions of the IgH locus (3' to JH segments, 5' to Cµ with Eµ deletion and Cα). The observed spectrum of tumors, kinetics of emergence, and transcriptome analysis provide strong evidence that both Eµ and 3'RR deregulate c-myc and cooperate together to promote B-cell lymphomagenesis. Transgenics mimicking endemic BL (with c-myc placed 3' to JH segments) exhibited the highest rate of B-cell lymphoma emergence, the highest Ki67 index of proliferation, and the highest transcriptomic similarities to human BL. The 3'RR enhancer alone deregulated c-myc and initiated the development of BL-like lymphomas, suggesting that its targeting would be of therapeutic interest to reduce c-myc oncogenicity in vivo.


Assuntos
Dromaiidae , Linfoma de Células B , Animais , Cadeias Pesadas de Imunoglobulinas/genética , Linfoma de Células B/genética , Camundongos , Camundongos Transgênicos , Sequências Reguladoras de Ácido Nucleico
19.
Braz J Biol ; 80(1): 66-72, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31116295

RESUMO

Emus are large flightless birds in the ratite group and are native to Australia. Since the mid-1980s, there has been increased interest in the captive breeding of emus for the production of leather, meat and oil. The aim of this study was to identify gastrointestinal parasites in the feces of emus Dromaius novaehollandiae from a South American scientific breeding. Fecal samples collected from 13 birds were examined by direct smears, both with and without centrifugation, as well as by the fecal flotation technique using Sheather's sugar solution. Trophozoites, cysts and oocysts of protozoa and nematode eggs were morphologically and morphometrically evaluated. Molecular analysis using PCR assays with specific primers for the genera Entamoeba, Giardia and Cryptosporidium were performed. Trophozoites and cysts of Entamoeba spp. and Giardia spp., oocysts of Eimeria spp. and Isospora dromaii, as well as eggs belonging to the Ascaridida order were found in the feces. Three animals were diagnosed with Giardia spp., and three were positive for Entamoeba spp. based on PCR techniques. After analyzing the data, we concluded that emus were infected enzootically by nematode and protozoan species.


Assuntos
Criptosporidiose , Cryptosporidium , Dromaiidae , Parasitos , Animais , Brasil , Fezes
20.
Curr Biol ; 29(21): R1131-R1133, 2019 11 04.
Artigo em Inglês | MEDLINE | ID: mdl-31689398

RESUMO

The vestigial wings of emus are a striking illustration of morphological evolution. A new study points to reduced activity of an essential signaling pathway as a factor in the evolution of the emu's stunted wings.


Assuntos
Dromaiidae , Animais , Membro Anterior , Transdução de Sinais , Asas de Animais
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...