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Sci Rep ; 13(1): 223, 2023 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-36604532


This study aimed to develop a predictive system for prognostic evaluation of osteosarcoma patients. We obtained osteosarcoma sample data from 1998 to 2016 using SEER*Stat software version 8.3.8, and established a multivariable Cox regression model using R-4.0.3 software. Data were extracted from the Surveillance, Epidemiology, and End Results (SEER) database. The diagnosis of the model was completed through influential cases, proportionality, and multicollinearity. The predictive ability of the model was tested using area under the curve (AUC), calibration curves, and Brier scores. Finally, the bootstrap method was used to internally verify the model. In total, data from 3566 patients with osteosarcoma were included in this study. The multivariate Cox regression model was used to determine the independent prognostic variables. A nomogram and Kaplan-Meier survival curve were established. The AUC and Brier scores indicated that the model had a good predictive calibration. In addition, we found that the radiotherapy appears to be a risk factor of patients with osteosarcoma and made a discussion. We developed a prognostic evaluation system for patients with osteosarcoma for 1-, 3-, and 5-year overall survival with good predictive ability using sample data extracted from the SEER database. This has important clinical significance for the early identification and treatment of high-risk groups of osteosarcoma patients.

Neoplasias Ósseas , Osteossarcoma , Humanos , Área Sob a Curva , Neoplasias Ósseas/mortalidade , Neoplasias Ósseas/radioterapia , Calibragem , Nomogramas , Osteossarcoma/mortalidade , Osteossarcoma/radioterapia , Prognóstico , Programa de SEER , Efeitos da Radiação
J Med Entomol ; 59(1): 320-327, 2022 01 12.
Artigo em Inglês | MEDLINE | ID: mdl-34595516


The sterilization process using gamma irradiation is a crucial component in a program using sterile insect technique (SIT) to control Aedes aegypti. Unfortunately, there is no efficient standard protocol for sterilizing mosquitoes that can produce a high level of sterility while maintaining mating ability and longevity. Therefore, we conducted a study of the critical factors necessary to develop such a standard protocol. In this study, male Ae. aegypti pupae, as well as adults aged 1 d and 3 d, were irradiated using a Gamma-cell 220 irradiator doses of 0, 20, 40, 60, 70, 80, and 100 Gray (Gy). In addition, male Ae. aegypti in the pupal and adult stage aged 1 d were irradiated at a dose of 70 Gy at various temperatures. Changes in emergence rates, longevity, sterility, and mating competitiveness were recorded for each combination of parameters. Results showed that an increase of irradiation dose leads to a rise of induced sterility at all developmental stages, while simultaneously reducing emergence rate, survival, and mating competitiveness. Higher temperatures resulted in increased levels of sterility, reduced longevity, and did not affect the ability to mate. This study found that an irradiation dose of 70 Gy at a temperature between 20.00 and 22.30°C administered in the pupal stage induced a high level of sterility (around 98%), while maintaining mating competitiveness and longevity.

Aedes/efeitos da radiação , Controle de Mosquitos/métodos , Esterilização/métodos , Animais , Longevidade , Mosquitos Vetores/efeitos da radiação , Pupa/efeitos da radiação , Radiação , Efeitos da Radiação , Fatores Sexuais , Comportamento Sexual Animal , Temperatura
Rev. medica electron ; 43(5): 1285-1296, 2021. tab
Artigo em Espanhol | LILACS, CUMED | ID: biblio-1352111


RESUMEN Introducción: el daño actínico crónico es un grupo de alteraciones en la estructura, función y apariencia de la piel como resultado de la exposición no controlada a las radiaciones ultravioletas. Puede provocar el cáncer de piel. Objetivo: caracterizar a los pacientes con daño actínico crónico, atendidos en la consulta de Dermatología del Hospital Comunitario Valle Hermoso, en el departamento de Cochabamba, Bolivia. Materiales y métodos: se realizó un estudio clínico descriptivo, prospectivo, en un universo de 1 833 pacientes diagnosticados con daño actínico crónico, atendidos en la consulta de Dermatología del Hospital Comunitario Valle Hermoso, en Cochabamba, entre septiembre de 2017 y septiembre de 2018. Se evaluaron las variables edad, sexo, color y fototipo de piel, ocupación, uso de medios de protección solar, exposición a otro tipo de radiaciones, manifestaciones clínicas de fotodaño y altitud del lugar de residencia. Resultados: predominaron el grupo de edad de 25 a 59 años, el sexo femenino, el color de piel mestizo (77,08 %), el fototipo de piel IV (76,98 %) y la ocupación comerciante (72,56 %). La mayoría de los pacientes (82,7 %) no utilizaron medios de protección solar, y el 99,8 % no tuvieron exposición a otro tipo de radiaciones. Las lesiones por fotodaño que prevalecieron fueron melasma (83,03 %) y lentigos (12,22 %). El 99,29 % vivían en zonas de gran altitud. Conclusiones: se caracterizaron los pacientes con daño actínico crónico, obteniendo en algunas variables estudiadas resultados similares a los mencionados por otros investigadores (AU).

ABSTRACT Introduction: chronic actinic damage is a group of alterations in the structure, function, and appearance of the skin as a result of uncontrolled exposure to ultraviolet radiation. It can cause skin cancer. Objective: to characterize the patients with chronic actinic damage, treated at the Dermatology consultation of Valle Hermoso Community Hospital, in the department of Cochabamba, Bolivia. Materials and methods: a descriptive, prospective clinical study was conducted in a universe of 1,833 patients diagnosed with chronic actinic damage, treated at the Dermatology clinic of the Valle Hermoso Community Hospital, Cochabamba, between September 2017 and September 2018. The variables age, sex, skin color, skin phototype, occupation, use of sun protectors, exposure to other types of radiation, clinical manifestations of photodamage and altitude of the place of residence were evaluated. Results: the age group from 25 to 59 years, the female sex, mestizo skin color (77.08 %), the IV skin phototype (76.98 %) and merchant occupation (72.56 %) predominated. Most patients (82.7 %) did not use sun protection means, and 99.8 % had no other radiation exposure. The prevailing photodamage lesions were melasma (83.03 %) and lentigo (12.22 %). 99.29 % lived in high altitude areas. Conclusions: the patients with chronic actinic damage were characterized, obtaining in some variables studied results similar to those mentioned by other researchers (AU).

Humanos , Masculino , Feminino , Pacientes/classificação , Transtornos de Fotossensibilidade/epidemiologia , Transtornos de Fotossensibilidade/diagnóstico , Efeitos da Radiação , Diagnóstico Clínico , Lentigo/diagnóstico , Melanose/diagnóstico
Rev. Ciênc. Méd. Biol. (Impr.) ; 20(1): 5-13, maio 5, 2021. tab, fig
Artigo em Inglês | LILACS | ID: biblio-1354757


Introduction: sunscreen is a cosmetic used to protect human skin from the deleterious effects of UV radiation and thus minimize the risks of developing skin cancer. Extracts produced from fruits, such as Abiu, Guava, Rambutan and Grape, have significant concentrations of chromophores and phenolic substances that have the ability to absorb UV light and neutralize free radicals. The production of cosmetics from naturally occurring substances with such activities will be of great importance for Brazilian industry and could reduce the cost of production, given the large existing biodiversity in the country. Objectives: carry out a patent search for formulations and cosmetic products that contains phenolic rich fruit extracts as photoprotective and antioxidant bioactives. Methodology: a survey of patents was carried out in the database of the National Institute of Intellectual Property (INPI) and the European Patent Office (EPO), covering a time period between 2008 and 2018, using a combination of keywords related to herbal cosmetics and sunscreens. Results: this research identifies a significant number of patents related to plant extract in association with sunscreen product, however, no patent filings were identified for cosmetic products containing extracts of the evaluated fruits, either alone or in combination, for photoprotective and antioxidant purposes. Conclusion: the Brazilian and international market for sun care products moves millions to billions of dollars annually and have a significant annual growth prospect. Considering the great biodiversity existing in the country, the production of cosmetics from naturally occurring substances with such activities will be of great importance for the Brazilian industrial sector.

Introdução: os filtros solares são cosméticos usados para proteger a pele humana dos efeitos deletérios da radiação UV e assim, minimizar os riscos de desenvolvimento de câncer de pele. Extratos produzidos a partir de frutos, como Abiu, Goiaba, Rambutan e Uva, têm concentrações significativas de cromóforos e substâncias fenólicas que têm a capacidade de absorver a radiação UV e neutralizar os radicais livres. A produção de cosméticos a partir de substâncias naturais com essas atividades será de grande importância para a indústria brasileira e poderá reduzir o custo de produção, dada a grande biodiversidade existente no país. Objetivos: realizar uma busca de registros de patentes de formulações e produtos cosméticos que contêm extratos de frutos ricos em fenólicos como bioativos fotoprotetores e antioxidantes. Metodologia: foi realizado um levantamento de patentes no banco de dados do Instituto Nacional de Propriedade Intelectual (INPI) e do Escritório Europeu de Patentes (EPO), abrangendo um período de tempo entre 2008 e 2018, utilizando uma combinação de palavras-chave relacionadas a cosméticos à base de plantas e protetores solares. Resultados: esta pesquisa identificou um número significativo de patentes relacionadas ao extrato de plantas em associação ao produto protetor solar, no entanto, não foram identificados pedidos de patente para produtos cosméticos que contenham extratos das frutas avaliadas, isoladamente ou em combinação, para fins fotoprotetores e antioxidantes. Conclusão: o mercado brasileiro e internacional de produtos de proteção solar movimenta milhões a bilhões de dólares anualmente e tem a perspectiva de crescimento anual significativa. Considerando a grande biodiversidade existente no país, a produção de cosméticos a partir de substâncias naturais com essas atividades será de grande importância para o setor industrial brasileiro.

Plantas , Efeitos da Radiação , Protetores Solares , Cosméticos , Compostos Fenólicos , Fator de Proteção Solar , Base de Dados
Eur J Epidemiol ; 36(4): 401-414, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33742296


Past reports indicated that total-body irradiation at low to moderate doses could be responsible for cardiovascular disease risks, but the mechanism remains unclear. The purpose of this study was to investigate the association between radiation exposure and atherosclerosis, an underlying pathology of cardiovascular diseases, in the Japanese atomic bomb survivors. We performed a cross-sectional study measuring 14 clinical-physiological atherosclerosis indicators during clinical exams from 2010 to 2014 in 3274 participants of the Adult Health Study cohort. Multivariable analyses were performed by using a structural equation model with latent factors representing underlying atherosclerotic pathologies: (1) arterial stiffness, (2) calcification, and (3) plaque as measured with indicators chosen a priori on the basis of clinical-physiological knowledge. Radiation was linearly associated with calcification (standardized coefficient per Gy 0.15, 95 % confidence interval: CI [0.070, 0.23]) and plaque (0.11, 95 % CI [0.029, 0.20]), small associations that were comparable to about 2 years of aging per Gy of radiation exposure, but not with arterial stiffness (0.036, 95 % CI [- 0.025, 0.095]). The model fitted better and had narrower confidence intervals than separate ordinary regression models explaining individual indicators independently. The associations were less evident when the dose range was restricted to a maximum of 2 or 1 Gy. By combining individual clinical-physiological indicators that are correlated because of common, underlying atherosclerotic pathologies, we found a small, but significant association of radiation with atherosclerosis.

Aterosclerose/etiologia , Sobreviventes de Bombas Atômicas , Efeitos da Radiação , Exposição à Radiação/efeitos adversos , Lesões por Radiação/complicações , Adulto , Idoso , Índice Tornozelo-Braço , Espessura Intima-Media Carotídea , Estudos Transversais , Humanos , Japão , Análise de Classes Latentes , Masculino , Pessoa de Meia-Idade , Armas Nucleares , Análise de Onda de Pulso
Int J Radiat Oncol Biol Phys ; 109(5): 1559-1569, 2021 04 01.
Artigo em Inglês | MEDLINE | ID: mdl-33321193


PURPOSE: Local ionizing radiation (IR) can lead to systemic lymphocyte depletion, which is associated with poor survival outcomes in patients with cancer. Interleukin-7 (IL-7) plays an important role in lymphocyte homeostasis; however, its role in alleviating radiation-induced lymphopenia remains unclear. Hence, we established a radiation-induced lymphopenia animal model and evaluated the effect of exogenous IL-7 administration. METHODS: C3H/HeN mice underwent x-ray irradiation of 30 Gy in 10 fractions at the right hind limbs. Next, 10 mg/kg of IL-7 was injected subcutaneously, and the lymphocyte count in blood was measured. Murine hepatocellular carcinoma (HCa-1) cells were inoculated subcutaneously into the right thighs of tumor model mice, which underwent the same treatment. RESULTS: In the naïve mouse model, the decreased CD45+ cell count after irradiation gradually recovered to the initial level over 3 weeks in the IR group, whereas it markedly increased to 373% of the initial level in 1 week in the IR+IL-7 group. Similar trends were observed for the CD3+, CD8+, CD4+, regulatory T cells, and CD19+ B cell counts. Similar findings were observed in the tumor mouse model. CD8+ and CD4+ T cell infiltration in tumor specimens was higher in the IL-7 and IR+IL-7 groups than in the nontreated and IR groups. Tumor growth was significantly more suppressed in the IR+IL-7 group than in the IR group. The median survival time was significantly longer in the IR+IL-7 group (not reached) than in the IR (56 days; P = .0382), IL-7 (36 days; P = .0004), or nontreated groups (36 days; P < .0001). CONCLUSIONS: Administration of exogenous IL-7 after IR not only restored lymphocyte counts but also enhanced the antitumor effect. Exogenous IL-7 can be beneficial in overcoming radiation-induced lymphopenia and in enhancing the treatment outcome in combination with radiation therapy, which needs validation through future clinical studies.

Linfócitos B , Interleucina-7/uso terapêutico , Depleção Linfocítica , Linfopenia/tratamento farmacológico , Linfócitos T , Animais , Linfócitos B/citologia , Linfócitos B/efeitos dos fármacos , Linfócitos B/imunologia , Linfócitos B/efeitos da radiação , Carcinoma Hepatocelular/tratamento farmacológico , Carcinoma Hepatocelular/imunologia , Carcinoma Hepatocelular/radioterapia , Terapia Combinada/métodos , Modelos Animais de Doenças , Ensaio de Imunoadsorção Enzimática , Imuno-Histoquímica , Neoplasias Hepáticas/tratamento farmacológico , Neoplasias Hepáticas/imunologia , Neoplasias Hepáticas/radioterapia , Contagem de Linfócitos , Linfopenia/etiologia , Masculino , Camundongos , Camundongos Endogâmicos C3H , Doses de Radiação , Efeitos da Radiação , Linfócitos T/citologia , Linfócitos T/efeitos dos fármacos , Linfócitos T/imunologia , Linfócitos T/efeitos da radiação , Ensaios Antitumorais Modelo de Xenoenxerto
Medicine (Baltimore) ; 99(52): e23766, 2020 Dec 24.
Artigo em Inglês | MEDLINE | ID: mdl-33350761


OBJECTIVES: This study aimed to evaluate the diagnostic performance of magnetic resonance perfusion-weighted imaging (PWI) as a noninvasive method to assess post-treatment radiation effect and tumor progression in patients with glioma. METHODS: A systematic literature search was performed in the PubMed, Cochrane Library, and Embase databases up to March 2020. The quality of the included studies was assessed by the quality assessment of diagnostic accuracy studies 2. Data were extracted to calculate sensitivity, specificity, and diagnostic odds ratio (DOR), 95% Confidence interval (CI) and analyze the heterogeneity of the studies (Spearman correlation coefficient, I2 test). We performed meta-regression and subgroup analyses to identify the impact of study heterogeneity. RESULTS: Twenty studies were included, with available data for analysis on 939 patients and 968 lesions. All included studies used dynamic susceptibility contrast (DSC) PWI, four also used dynamic contrast-enhanced PWI, and three also used arterial spin marker imaging PWI. When DSC was considered, the pooled sensitivity and specificity were 0.83 (95% CI, 0.79 to 0.86) and 0.83 (95% CI, 0.78 to 0.87), respectively; pooled DOR, 21.31 (95% CI, 13.07 to 34.73); area under the curve (AUC), 0.887; Q∗, 0.8176. In studies using dynamic contrast-enhanced, the pooled sensitivity and specificity were 0.73 (95% CI, 0.66 to 0.80) and 0.80 (95% CI, 0.69 to 0.88), respectively; pooled DOR, 10.83 (95% CI, 2.01 to 58.43); AUC, 0.9416; Q∗, 0.8795. In studies using arterial spin labeling, the pooled sensitivity and specificity were 0.79 (95% CI, 0.69 to 0.87) and 0.78 (95% CI, 0.67 to 0.87), respectively; pooled DOR, 15.63 (95% CI, 4.61 to 53.02); AUC, 0.8786; Q∗, 0.809. CONCLUSIONS: Perfusion magnetic resonance imaging displays moderate overall accuracy in identifying post-treatment radiation effect and tumor progression in patients with glioma. Based on the current evidence, DSC-PWI is a relatively reliable option for assessing tumor progression after glioma radiotherapy.

Neoplasias Encefálicas , Glioma , Angiografia por Ressonância Magnética/métodos , Neoplasias Encefálicas/diagnóstico , Neoplasias Encefálicas/radioterapia , Progressão da Doença , Glioma/diagnóstico , Glioma/radioterapia , Humanos , Estadiamento de Neoplasias , Efeitos da Radiação
Rev. esp. med. nucl. imagen mol. (Ed. impr.) ; 39(5): 303-315, sept.-oct. 2020. graf
Artigo em Espanhol | IBECS | ID: ibc-198292


El modelo lineal sin umbral (MLSU) es una función dosis-respuesta teórica obtenida de extrapolar los efectos tardíos debidos a la exposición a altas dosis de radiación ionizante al rango de las bajas dosis, pero existen grandes incertidumbres respecto a su validez. La aceptación del MLSU como modelo probabilístico preponderante ha sobrevivido hasta nuestros días y constituye la piedra angular que sostiene las políticas actuales de protección radiológica. En las últimas décadas, los avances en biología molecular y evolutiva, en la inmunología del cáncer, así como los resultados obtenidos de los estudios epidemiológicos y en modelos animales, han puesto en entredicho la fiabilidad del MLSU en favor de otras alternativas, como la teoría hormética. A la vista de las evidencias, se hace necesario un debate entre las sociedades científicas implicadas y los organismos reguladores que aborde una redefinición de las bases de la protección radiológica, cuya importancia sería capital en el ámbito médico

The linear non-threshold model (LNTM) is a theoretical dose-response function as a result of extrapolating the late effects of high-dose exposure to ionizing radiation to the low-dose range, but there is great uncertainty about its validity. The acceptance of LNTM as the dominant probabilistic model have survived to the present day and it is actually the cornerstone of current radiation protection policies. In the last decades, advances in molecular and evolutive biology, cancer immunology, and many epidemiological and animal studies have cast serious doubts about the reliability of the NLTM, as well as suggesting alternative models, like the hormetic theory. Considering the given evidences, a discussion between the involved scientific societies and the regulatory commissions is promtly required in order to to reach a redefiniton of theradiation protection basis, as it would be specially crucial in the medical field

Humanos , Relação Dose-Resposta à Radiação , Hormese/efeitos da radiação , Radiobiologia/métodos , Carcinogênese/efeitos da radiação , Lesões por Radiação/prevenção & controle , 51569/prevenção & controle , Proteção Radiológica/normas , Doses de Radiação , Fracionamento da Dose de Radiação , Modelos Lineares , Medicina Nuclear/métodos , Radiação Ionizante/classificação , Efeitos Adversos de Longa Duração/prevenção & controle , Efeitos da Radiação , Radiometria/métodos
Isr J Health Policy Res ; 9(1): 34, 2020 06 30.
Artigo em Inglês | MEDLINE | ID: mdl-32605644


BACKGROUND: Until 1960, hundreds of thousands of children worldwide had been treated for scalp ringworm by epilation via irradiation. The discovery of late health effects in adulthood prompted investigation of the medical aspects of irradiation in childhood and led to the establishment of strict protocols for the use of X-ray irradiation. These studies ignored alopecia, which affects some individuals who underwent irradiation for scalp ringworm as children. This study examined the impact of alopecia due to irradiation for scalp ringworm on the health and psychosocial status of affected women. METHODS: We analysed a random sample of 130 medical files of women recognised by Israel's state committees as suffering from permanent hair loss as a result of scalp ringworm irradiation in childhood. The coded medical data included demographic variables, self-reported mental health conditions, self-reported physical health conditions, self-reported social conditions, and spousal relationship. RESULTS: Compared with the general population of women in Israel, research participants reported significantly higher rates of depression, anti-depressant and/or anti-anxiety drug use, psychotherapy or psychiatric hospitalisation, attempted suicide, migraines, cancer, and divorce. Many described humiliating social experiences due to their appearance, both in childhood and adulthood, that led them to curtail their social interactions. The participants also reported that alopecia negatively affected their spousal relationships. CONCLUSIONS: Life with hair loss from scalp ringworm irradiation in childhood has a negative impact on women's health status and psychosocial state. Health policy-makers must broaden their approach to women who underwent scalp ringworm irradiation by addressing the effects of their hair loss in addition to the effects of the radiation treatment per se. This may be achieved by guiding physicians who provide medical services to these women to take into account the psychosocial and health risks related to hair loss in their diagnosis and treatment as well as by creating a cadre of specially trained mental health professionals who can address their unique psychosocial needs. They must also consider including the specialized mental health services tailored for these women's unique needs in the Healthcare Basket.

Alopecia/etiologia , Efeitos da Radiação , Tinha/complicações , Tinha/terapia , Adulto , Idoso , Idoso de 80 Anos ou mais , Alopecia/fisiopatologia , Feminino , Humanos , Israel , Pessoa de Meia-Idade , Comportamento Sexual/psicologia , Tinha/fisiopatologia
IEEE Pulse ; 11(2): 17-19, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32386133


Despite the advances in developing nuclear weapons and other technologies, not much is known about the long-term effects of radiation on human health. In a world where nuclear energy could help curb carbon emissions, it almost seems paradoxical that its possible long-term risks and impacts to human health are still poorly understood. While researchers have investigated the effects of acute radiation in the aftermath of nuclear incidents such as Chernobyl, Hiroshima, Nagasaki, and Fukushima, more needs to be done to understand the impacts of long-term and low-dose nuclear radiation. Studying the downwinders from the Nevada Test Site could offer one window to investigate those myriad effects.

Estudos de Associação Genética , Medicina de Precisão , Efeitos da Radiação , Exposição à Radiação , Humanos , Japão , Neoplasias/genética , Armas Nucleares , Liberação Nociva de Radioativos , Radiobiologia , Sudoeste dos Estados Unidos , Ucrânia
J. nurs. health ; 10(2): 20102009, mai.2020.
Artigo em Português | LILACS, BDENF - Enfermagem | ID: biblio-1104137


Objetivo: analisar e categorizar o método de uso e benefícios e principais considerações sobre uso do laser em lesões induzidas por radiação. Método: revisão sistemática desenvolvidas em Public Medline, Literatura Latino-Americana do Caribe em Ciências da Saúde, Cumulative Index to Nursing and Allied Health Literature, SCOPUS e Biblioteca Virtual de Saúde. Resultados: analisou-se sete estudos. Observou-se em 86% (6) desses estudos uma conclusão positiva à terapêutica de laser de baixa intensidade, relatando: 43% (3) diminuição significativa e regressão da dor; 29% (2) redução significativa na duração e gravidade da mucosite e 29% (2) redução da inflamação e cicatrização. Conclusão: evidenciou-se melhora da cicatrização, da dor local e da capacidade anti-inflamatória. É necessário realizar mais pesquisas com amostras robustas que comparem os tipos de lasers, o comprimento das ondas e sua intensidade, para o seu uso como terapêutica.(AU)

Objective: to analyze and categorize the method of use and benefits and main considerations on the use of laser in radiation-induced injuries. Method: systematic review developed in the databases Public Medline, Latin American Caribbean Literature in Health Sciences, Cumulative Index to Nursing and Allied Health Literature, SCOPUS and Virtual Health Library. Results: seven studies were analyzed. A positive conclusion to low-level laser therapy was observed in 86% (6) of these studies, reporting: 43% (3) significant decrease and regression of pain; 29% (2) significant reduction in the duration and severity of mucositis and 29% (2) reduction in inflammation and healing. Conclusion: there was an improvement in healing, local pain and anti-inflammatory capacity. More research is needed with robust samples that compare the types of lasers, the length of the waves and their intensity, for their use as therapy.(AU)

Objetivo: analizar y clasificar el método de uso y los beneficios y las principales consideraciones sobre el uso del láser en lesiones inducidas por radiación. Método: revisión sistemática desarrollada en Public Medline, Literatura Latinoamericana del Caribe en Ciencias de la Salud, Índice acumulativo de literatura de enfermería y salud aliada, SCOPUS y Biblioteca Virtual en Salud. Resultados: se analizaron siete estudios. Se observó una conclusión positiva de la terapia con láser de bajo nivel en el 86% (6) de estos estudios: 43% (3) disminución significativa y regresión del dolor; 29% (2) reducción significativa en la duración y gravedad de la mucositis y 29% (2) reducción en la inflamación y la curación. Conclusión: hubo una mejora en la curación, dolor local y capacidad antiinflamatoria. Se necesita investigación con muestras robustas que comparen los tipos de láser, la longitud de las ondas y su intensidad, para su uso como terapia.(AU)

Efeitos da Radiação , Radiodermatite , Radioterapia , Terapia com Luz de Baixa Intensidade
Sci Rep ; 10(1): 37, 2020 01 08.
Artigo em Inglês | MEDLINE | ID: mdl-31913321


The exposure of germ cells to radiation introduces mutations in the genomes of offspring, and a previous whole-genome sequencing study indicated that the irradiation of mouse sperm induces insertions/deletions (indels) and multisite mutations (clustered single nucleotide variants and indels). However, the current knowledge on the mutation spectra is limited, and the effects of radiation exposure on germ cells at stages other than the sperm stage remain unknown. Here, we performed whole-genome sequencing experiments to investigate the exposure of spermatogonia and mature oocytes. We compared de novo mutations in a total of 24 F1 mice conceived before and after the irradiation of their parents. The results indicated that radiation exposure, 4 Gy of gamma rays, induced 9.6 indels and 2.5 multisite mutations in spermatogonia and 4.7 indels and 3.1 multisite mutations in mature oocytes in the autosomal regions of each F1 individual. Notably, we found two types of deletions, namely, small deletions (mainly 1~12 nucleotides) in non-repeat sequences, many of which showed microhomology at the breakpoint junction, and single-nucleotide deletions in mononucleotide repeat sequences. The results suggest that these deletions and multisite mutations could be a typical signature of mutations induced by parental irradiation in mammals.

Genoma , Mutação , Oócitos/fisiologia , Espermatogônias/fisiologia , Animais , Animais Recém-Nascidos , Feminino , Raios gama , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Taxa de Mutação , Oócitos/efeitos da radiação , Efeitos da Radiação , Radiação Ionizante , Espermatogônias/efeitos da radiação , Sequenciamento Completo do Genoma
Front Immunol ; 11: 617510, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33552081


TLRs, key components of the innate immune system, recognize microbial molecules. However, TLRs also recognize some nonmicrobial molecules. In particular, TLR2 and TLR4 recognize hyaluronic acid, a glycosaminoglycan in the extracellular matrix. In neonatal mice endogenous hyaluronic acid binding to TLR4 drives normal intestinal growth. Hyaluronic acid binding to TLR4 in pericryptal macrophages results in cyclooxygenase2- dependent PGE2 production, which transactivates EGFR in LGR5+ crypt epithelial stem cells leading to increased proliferation. The expanded population of LGR5+ stem cells leads to crypt fission and lengthening of the intestine and colon. Blocking this pathway at any point (TLR4 activation, PGE2 production, EGFR transactivation) results in diminished intestinal and colonic growth. A similar pathway leads to epithelial proliferation in wound repair. The repair phase of dextran sodium sulfate colitis is marked by increased epithelial proliferation. In this model, TLR2 and TLR4 in pericryptal macrophages are activated by microbial products or by host hyaluronic acid, resulting in production of CXCL12, a chemokine. CXCL12 induces the migration of cyclooxygenase2-expressing mesenchymal stem cells from the lamina propria of the upper colonic crypts to a site adjacent to LGR5+ epithelial stem cells. PGE2 released by these mesenchymal stem cells transactivates EGFR in LGR5+ epithelial stem cells leading to increased proliferation. Several TLR2 and TLR4 agonists, including hyaluronic acid, are radioprotective in the intestine through the inhibition of radiation-induced apoptosis in LGR5+ epithelial stem cells. Administration of exogenous TLR2 or TLR4 agonists activates TLR2/TLR4 on pericryptal macrophages inducing CXCL12 production with migration of cyclooxygenase2-expressing mesenchymal stem cells from the lamina propria of the villi to a site adjacent to LGR5+ epithelial stem cells. PGE2 produced by these mesenchymal stem cells, blocks radiation-induced apoptosis in LGR5+ epithelial stem cells by an EGFR mediated pathway.

Intestinos/crescimento & desenvolvimento , Efeitos da Radiação , Cicatrização/fisiologia , Animais , Proliferação de Células/fisiologia , Humanos , Mucosa Intestinal/metabolismo , Receptores Toll-Like/metabolismo
Rev. esp. cir. oral maxilofac ; 41(2): 61-67, abr.-jun. 2019. ilus, tab, graf
Artigo em Espanhol | IBECS | ID: ibc-191460


INTRODUCCIÓN: El colgajo peroneo es actualmente una de las técnicas más elegidas para la reconstrucción de defectos mandibulares. Muchos de los pacientes reconstruidos con este colgajo presentan patología oncológica que precisa tratamiento adyuvante con radioterapia. Los efectos adversos de la radioterapia sobre los tejidos son conocidos, pudiendo desembocar en osteoradionecrosis. El objetivo de este estudio es el análisis de la densidad ósea de los colgajos peroneos en los pacientes que han recibido radioterapia frente a los que no y su posible correlación con las complicaciones posteriores. MATERIAL Y MÉTODOS: Realizamos un estudio retrospectivo de los colgajos peroneos realizados por nuestro servicio desde enero 2011 hasta diciembre 2016. Los criterios de inclusión de los pacientes fue el haber sido reconstruidos con un colgajo peroneo y disponer de un TC de control a los tres meses postradioterapia en caso de haberla precisado. Se recogió la edad, densidad ósea peronea, desviación estándar cuerpo mandibular remanente y colgajo peroneo, así como las complicaciones postoperatorias. Los datos extraídos fueron analizados a través del Software SAS (Statistical Analisys System). RESULTADOS: En total se recogieron 61 pacientes, siendo la media de edad de 54,46 años. De estos 61 pacientes se excluyeron 11 al no presentar TC de control; de los 50 pacientes restantes, 27 recibieron tratamiento radioterápico adyuvante (54 %), mientras que 23 no la recibieron (46 %). En los pacientes irradiados, un alto porcentaje (84,62 %) recibieron altas dosis de radioterapia (> 60 Gy). Se encontraron diferencias estadísticamente significativas (p < 0,05) respecto a la edad y la desviación estándar de la densidad ósea del cuerpo mandibular remanente, mientras que en el resto de los parámetros no se encontraron diferencias estadísticamente significativas. Los pacientes irradiados presentaron un mayor porcentaje de complicaciones frente a los pacientes no irradiados, sin diferencias estadísticamente significativas. Discusión: Las nuevas técnicas de radioterapia de intensidad modulada permiten una dosis relativamente uniforme en un objetivo, evitando altas dosis en los tejidos circuncidantes. A pesar del avance en las técnicas de radioterapia, actualmente en la mayoría de centros se continúa realizando un tratamiento completo del volumen del lecho tumoral, que incluye en el campo de irradiación el colgajo con el que se reconstruye. Los casos de osteonecrosis mandibular que se presentaron fueron en pacientes irradiados y a nivel del cuerpo mandibular remanente. La densidad ósea a nivel del colgajo peroneo sí que presentó diferencias estadísticamente significativas en pacientes irradiados frente a no irradiados; este aspecto puede influir en el índice de complicaciones. CONCLUSIONES: Consideramos, con los resultados del estudio, plantear nuevos estudios prospectivos para valorar la necesidad de irradiar el tejido óseo del colgajo peroneo como parte del volumen del lecho tumoral, siendo que este tejido no ha estado en contacto con el tumor primario, ante el mayor índice de complicaciones asociados a la radioterapia en estos tejidos

INTRODUCTION: The fibular flap is currently one of the most chosen techniques for the reconstruction of mandibular defects. Many of the patients reconstructed with this flap present oncological pathology that requires adjuvant treatment with radiotherapy. The adverse effects of radiotherapy on tissues are known, and can lead to osteoradionecrosis. The aim of this study is to analyze the bone density of peroneal flaps in patients who have received radiotherapy against those who do not and their possible correlation with subsequent complications. MATERIAL AND METHODS: We designed a retrospective study of the fibular flaps performed by our service from January 2011 to December 2016. The criteria for inclusion of patients was to have been reconstructed with a peroneal flap and have a control CT at 3 months after radiotherapy. Age, bone density, standard deviation and further complications were extracted. The extracted data was analyzed through the SAS (statistical Analisys System) Software. RESULTS: 61 patients were collected; the mean age was of 54.46 years. Of these 61 patients were excluded 11 by not presenting control TC, of the 50 patients remaining, 27 received adjuvant radiotherapy treatment (54 %) while 23 did not receive (46 %). In irradiated patients a high percentage (84.62 %) received high doses of radiotherapy (> 60 Gy). Statistically significant differences were found (P < 0.05) with respect to age and standard deviation of bone density of the remaining mandibular body, while in the rest of the parameters no statistical significant differences were found. Irradiated patients presented a higher percentage of complications compared to non-irradiated patients without statistically significant differences. Discussion: The new techniques of intensity-modulated radiotherapy allow a relatively uniform dose in a target, avoiding high doses in the circumcising tissues. Despite the progress in radiotherapy techniques, currently in most centers, it continues to perform a complete treatment of the volume of the tumor bed that includes in the field of irradiation the flap with which it is reconstructed. The cases of mandibular osteonecrosis that were presented were in irradiated patients and in the remaining mandible. Bone density at the level of the fibular flap showed statistically significant differences in irradiated patients compared to non-irradiated, this aspect may influence in the index of complications. CONCLUSIONS: We consider to propose new prospective studies to assess the need to irradiate the bone tissue of the fibular flap as part of the volume of the tumor bed, knowing that this tissue has not been in contact with the primary tumor, and the high index of complications associated with radiotherapy

Humanos , Retalhos Cirúrgicos/imunologia , Fíbula/transplante , Reconstrução Mandibular/métodos , Neoplasias Mandibulares/radioterapia , Estudos Retrospectivos , Radioterapia/estatística & dados numéricos , Osteorradionecrose/epidemiologia , Resultado do Tratamento , Efeitos da Radiação
Artigo em Espanhol | BINACIS | ID: biblio-1094944


INTRODUCCIÓN: Hasta el momento, los resultados de distintas investigaciones sobre la exposición a Radiofrecuencias y sus consecuencias biológicas, son contradictorios. Nuestro estudio nace como un estudio de replicación, en conjunto con investigadores de la Escuela de Tecnología de la Salud de Lisboa, Lisboa, Portugal. Adoptamos el mismo diseño de estudio, para verificar si los resultados de su evaluación de la frecuencia de micronucleos (MN) en los usuarios de teléfonos móviles eran comparables a los resultados obtenidos en este estudio similar en Córdoba, Argentina. MATERIALES Y METODOS: Para estudiar el daño del ADN por la exposición a radiofrecuencia asociada con el teléfono celular se estudiaron los MN (marcadores celulares de daño genómico) en células bucales exfoliadas, extraídas de frotis de células de las mejillas internas derecha e izquierda de usuarios de teléfonos celulares en 83 voluntarios sanos de 18 a 36 años. Las frecuencias de MN fueron analizadas por la duración y el lado preferencial de uso del teléfono celular. RESULTADOS: no se observó relación entre la frecuencia de MN y la duración del uso como tampoco con el lado preferencial. CONCLUSION: nuestros resultados sugieren que la exposición a radiofrecuencia en los niveles observados no induce la formación de MN en células bucales. (AU)

INTRODUCTION: the results of various research on radiofrequency exposure and its biological consequences are contradictory. Taking into account this reasoning, our study was born as a replication study, together with researchers from the School of Health Technology of Lisbon, Lisbon, Portugal. We adopted the same study design, to verify if the results of your evaluation of the frequency of micronuclei (MN) in cell phone users were comparable to the results obtained in this similar study in Cordoba, Argentina. MATERIALS AND METHODS: To study ADN damage from radiofrequency exposure associated with cell phone, MN (cell markers of genomic damage) were studied in exfoliated buccal cells, extracted from right and left internal cheek cell smears of cell phone in 83 healthy volunteers from 18 to 36 years. RESULTS: The MN frequencies were analyzed by the duration and the preferential side of use of the cell phone. No relationship was observed between the MN frequency and the duration of use as well as the preferential side. CONCLUSION: our results suggest that exposure to radiofrequency in the observed levels does not induce the formation of MN in buccal cells. (AU)

Humanos , Adolescente , Adulto , Telefone Celular , Radiação Eletromagnética , Mucosa Bucal/efeitos da radiação , Efeitos da Radiação
BMC Genomics ; 20(1): 354, 2019 May 09.
Artigo em Inglês | MEDLINE | ID: mdl-31072350


BACKGROUND: Evolution leaves an imprint in species through genetic change. At the molecular level, evolutionary changes can be explored by studying ratios of nucleotide substitutions. The interplay among molecular evolution, derived phenotypes, and ecological ranges can provide insights into adaptive radiations. Caecilians (order Gymnophiona), probably the least known of the major lineages of vertebrates, are limbless tropical amphibians, with adults of most species burrowing in soils (fossoriality). This enigmatic order of amphibians are very distinct phenotypically from other extant amphibians and likely from the ancestor of Lissamphibia, but little to nothing is known about the molecular changes underpinning their radiation. We hypothesised that colonization of various depths of tropical soils and of freshwater habitats presented new ecological opportunities to caecilians. RESULTS: A total of 8540 candidate groups of orthologous genes from transcriptomic data of five species of caecilian amphibians and the genome of the frog Xenopus tropicalis were analysed in order to investigate the genetic machinery behind caecilian diversification. We found a total of 168 protein-coding genes with signatures of positive selection at different evolutionary times during the radiation of caecilians. The majority of these genes were related to functional elements of the cell membrane and extracellular matrix with expression in several different tissues. The first colonization of the tropical soils was connected to the largest number of protein-coding genes under positive selection in our analysis. From the results of our study, we highlighted molecular changes in genes involved in perception, reduction-oxidation processes, and aging that likely were involved in the adaptation to different soil strata. CONCLUSIONS: The genes inferred to have been under positive selection provide valuable insights into caecilian evolution, potentially underpin adaptations of caecilians to their extreme environments, and contribute to a better understanding of fossorial adaptations and molecular evolution in vertebrates.

Proteínas de Anfíbios/genética , Anfíbios/genética , Evolução Molecular , Efeitos da Radiação , Seleção Genética , Proteínas de Anfíbios/efeitos da radiação , Anfíbios/classificação , Animais , Genoma , Anotação de Sequência Molecular , Fenótipo , Filogenia
Rev. luna azul ; 49(0): 200-219, 2019. tab, ilus
Artigo em Espanhol | LILACS | ID: biblio-1121056


Introducción. Este trabajo de investigación se realizó en la Ciudad de Honda, Colombia. Identificó el efecto de la sombra de los árboles en la atenuación de la temperatura, humedad relativa, temperatura de superficie del suelo, radiación solar y ultravioleta (UV) y determinó requerimientos de agua para la vegetación arbórea. Metodología. Se tomaron datos de temperatura del aire y de superficie de suelo, humedad relativa del aire, radiaciones solares y UV bajo la sombra de árboles y a plena exposición. Resultados. Se encontró que la temperatura del aire osciló entre 50ºC a plena exposición solar y 41ºC bajo sombra. La temperatura de superficie presentó a plena exposición solar valores de 66.8ºC y 42.6ºC bajo sombra. La variación de la radiación solar registrada ofreció un valor de 17.13 mW/cm2 a plena exposición solar y 1.69 mW/cm2 bajo sombra. La radiación UV presentó valores de 8 a plena exposición solar y 5 bajo sombra. La demanda hídrica por planta/día varió entre 5 y más de 500 litros, siendo la demanda diaria cercana a 2500 m3 . Conclusiones. En la ciudad no se realiza riego a los árboles, lo que conduce a que se produzca un déficit hídrico evidenciado por la pérdida de follaje ocasionando mínimas atenuaciones a la radiación UV. Las especies más relevantes respecto a la sombra son almendro (Terminalia cattapa), Pallandé (Pitecellobium dulce), naranjuelo (Capparis odoratissima), guayacán carrapo (Bulnesia carrapo), chirlobirlo (Tecoma stands), cumulá (Aspidosperma polyneuron) y mango (Manguifera indica). Los árboles pueden generar un buen servicio ecosistémico por la sombra, este servicio está mediado por la selección de la especie y el manejo de que son objeto los árboles.

Introduction: This research work was carried out in the city of Honda, Colombia. It identified the effect of tree shade on temperature attenuation, relative humidity, soil surface temperature, solar and ultraviolet (UV) radiation, and determined water requirements for arboreal vegetation. Methodology: Data on air and soil surface temperature, relative air humidity, solar and UV radiation were taken under the shade of trees and at full exposure. Results: It was found that the air temperature ranged from 50ºC at full sun exposure to 41ºC under shade. The surface temperature was 66.8ºC and 42.6ºC under shade. The variation of the registered solar radiation offered a value of 17.13 mW/cm2 at full solar exposure and 1.69 mW/cm2 under shade. The UV radiation presented values of 8 at full solar exposure and 5 under shade. The water demand per plant/day varied between 5 and more than 500 liters, being the daily demand close to 2500 m3. Conclusions: There is no irrigation of trees in the city which leads to a water deficit evidenced by the loss of foliage causing minimal attenuations to UV radiation. The most relevant species regarding shade are almond (Terminalia cattapa), Pallandé (Pitecellobium dulce), orange (Capparis odoratissima), guayacán carrapo (Bulnesia carrapo), chirlobirlo (Tecoma stands), cumula (Aspidosperma polyneuron) and mango (Manguifera indica). Trees can generate a good ecosystem service by shade which is mediated by the selection of the species and the management of the trees.

Humanos , Cidades , Efeitos da Radiação , Área Urbana , Ecologia