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1.
Artigo em Inglês | MEDLINE | ID: mdl-34499598

RESUMO

A novel species within the genus Eikenella is described, based on the phenotypical, biochemical and genetic characterization of a strain of a facultatively anaerobic, Gram-negative rod-shaped bacterium. Strain S3360T was isolated from the throat swab of a patient sampled during routine care at a hospital. Phylogenetic analyses (full-length 16S rRNA gene and whole-genome sequences) placed the strain in the genus Eikenella, separate from all recognized species but with the closest relationship to Eikenella longinqua (NML 02-A-017T). Eikenella is one of the genera in the HACEK group known to be responsible for rare cases of endocarditis in humans. Until the recent descriptions of Eikenella exigua, Eikenella halliae and Eikenella longinqua, Eikenella corrodens had been the only validly published species in this genus since its description as Bacteroides corrodens in 1958. Unlike these species, strain S3360T is able to metabolize carbohydrates (glucose). The average nucleotide identities of strain S3360T with E. longinqua (NML 02-A-017T) and E. corrodens (NCTC 10596T), the type species of the genus, were 90.5 and 84.7 %, respectively, and the corresponding genome-to-genome distance values were 41.3 and 29.0 %, respectively. The DNA G+C content of strain S3360T was 58.4 mol%. Based on the phenotypical, biochemical and genetic findings, strain S3360T is considered to represent a novel species within the genus Eikenella, for which the name Eikenella glucosivorans sp. nov. is proposed. The type strain is S3360T (DSM 110714T=CCOS 1935T=CCUG 74293T). In addition, an emendation of the genus Eikenella is proposed to include species which are saccharolytic.


Assuntos
Eikenella , Faringe , Filogenia , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Eikenella/classificação , Eikenella/isolamento & purificação , Ácidos Graxos/química , Humanos , Faringe/microbiologia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
2.
Int J Syst Evol Microbiol ; 70(5): 3167-3178, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32302276

RESUMO

The Haemophilus, Aggregatibacter, Cardiobacterium, Eikenella, Kingella (HACEK) group genus Eikenella contained a single species, Eikenella corrodens, for many years. In November 2019, Eikenella exigua was described after recovery from a brain abscess and blood culture in Norway. Coincidentally, characterization of 22 Gram-negative bacteria resembling Eikenella from 17 Canadian patients had been underway. Seven isolates from five patients were conclusively identifiable as E. corrodens. One (NML 120819) was deemed to represent a species of the genus Eikenella most closely related to E. corrodens. Fourteen isolates had 97.6 to 98.8% similarities to E. corrodens by 16S rRNA gene sequencing, forming three distinct groups by genome analyses. The largest contained ten anaerobic isolates from eight patients recovered from blood, brain, bone and other abscesses; upon re-evaluation, this group was found to be most consistent with E. exigua. A second facultatively anaerobic clade consisted of two ocular isolates from one patient and a sinus isolate from a second patient. The third taxon consisted of a single strictly anaerobic blood culture isolate. The novel taxa, like E. corrodens, were poorly reactive biochemically and difficult to discern from each other phenotypically and chemotaxonomically, including by cellular fatty acids. MALDI-TOF (Bruker) and whole-genome sequencing were used to further characterize isolates. Draft genomes for the strains had similar DNA G+C contents (55.38-58.53 mol%) while sizes varied from 1.82 Mb to 2.54 Mb. We propose here emendations of the genus Eikenella and the species Eikenella exigua, as well as describing Eikenella halliae sp. nov. NML 130454T (=LMG 30894T=NCTC 14180T) and Eikenella longinqua sp. nov. NML 02-A-017T (=LMG 30896T=NCTC 14179T), on the basis of these findings.


Assuntos
Sangue/microbiologia , Eikenella/classificação , Filogenia , Bactérias Anaeróbias/classificação , Técnicas de Tipagem Bacteriana , Composição de Bases , Hemocultura , Canadá , DNA Bacteriano/genética , Eikenella/isolamento & purificação , Ácidos Graxos/química , Humanos , Noruega , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
4.
Int J Syst Evol Microbiol ; 70(3): 1478-1488, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31693476

RESUMO

We herein describe the first novel species within the genus Eikenella since it was established in 1972 by the reclassification of 'Bacteroides corrodens' to Eikenella corrodens. From a polymicrobial brain abscess, we encountered an Eikenella isolate, PXXT, that could not validly be named E. corrodens. The isolate grew on blood agar with small, translucent, pitting colonies after 3 days of anaerobic incubation. By reviewing previously collected invasive isolates, we found an additional Eikenella strain, EI-02, from a blood culture exhibiting the same properties as PXXT. Phylogenetic analyses based on both whole genome and individual house-keeping genes confirmed that the two strains allocate in a phylogenetic cluster separate from E. corrodens. Using specific amplification and sequencing of the Eikenella nusG gene, we further detected the novel Eikenella species in six historic brain abscesses previously reported to contain E. corrodens based on 16S metagenomics. Out of 24 Eikenella whole-genome projects available in GenBank, eight cluster together with PXXT and EI-02. These isolates were recovered from brain abscess (n=2), blood (n=1), bone/soft tissue (n=3), parotid gland (n=1) and unknown (n=1). It remains to be investigated whether the new species can cause endocarditis. The average nucleotide identity value between strain PXXT and the E. corrodens type strain ATCC 23834T was 92.1 % and the corresponding genome-to-genome distance value was 47.1 %, both supporting the classification of PXXT as a novel species. For this species we propose the name Eikenella exigua. The type strain of E. exigua is PXXT (DSM 109756T, NCTC 14318T).


Assuntos
Abscesso Encefálico/microbiologia , Eikenella/classificação , Filogenia , Técnicas de Tipagem Bacteriana , Composição de Bases , Hemocultura , DNA Bacteriano/genética , Eikenella/isolamento & purificação , Humanos , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
5.
Microbes Infect ; 21(2): 109-112, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-30385304

RESUMO

Sialolithiasis represents the most common disorders of salivary glands in middle-aged patients. It has been hypothesized that the retrograde migration of bacteria from the oral cavity to gland ducts may facilitate the formation of stones. Thus, in the present study, a microbiome characterization of salivary calculi was performed to evaluate the abundance and the potential correlations between microorganisms constituting the salivary calculi microbiota. Our data supported the presence of a core microbiota of sialoliths constituted principally by Streptococcus spp., Fusobacterium spp. and Eikenella spp., along with the presence of important pathogens commonly involved in infective sialoadenitis.


Assuntos
Biofilmes , Eikenella/fisiologia , Fusobacterium/fisiologia , Microbiota/fisiologia , Cálculos Salivares/microbiologia , Sialadenite/microbiologia , Streptococcus/fisiologia , Idoso , Eikenella/isolamento & purificação , Feminino , Fusobacterium/isolamento & purificação , Humanos , Masculino , Pessoa de Meia-Idade , Cálculos das Glândulas Salivares/microbiologia , Streptococcus/isolamento & purificação
6.
Enferm. infecc. microbiol. clín. (Ed. impr.) ; 35(supl.3): 29-43, oct. 2017. ilus, tab
Artigo em Espanhol | IBECS | ID: ibc-170748

RESUMO

Las bacterias del grupo HACEK (Haemophilus, Aggregatibacter, Cardiobacterium, Eikenella, Kingella), Pasteurella y Capnocytophaga son las bacterias gramnegativas de crecimiento lento que con mayor frecuencia causan infecciones en el ser humano. Forman parte de la microbiota del tracto respiratorio superior y genitourinario del ser humano y de animales, y pueden causar infecciones en cualquier localización, pero fundamentalmente de piel y tejidos blandos, así como bacteriemia y endocarditis. Su clasificación taxonó- mica es compleja y está en constante revisión. Son bacterias nutricionalmente exigentes, y para el desarrollo de colonias visibles requieren agar sangre y agar chocolate, una atmósfera aerobia, generalmente enriquecida en CO2 y una incubación de 48 h. La identificación fenotípica de especie es complicada y no siempre es posible, ya que requiere múltiples sustratos que normalmente no están disponibles en los laboratorios de rutina, ni en los sistemas automatizados. La aplicación de las técnicas moleculares y proteómicas ha permitido una mejor identificación de estas bacterias. El tratamiento de estas infecciones se encuentra con el problema de que los datos de sensibilidad a los agentes antimicrobianos son limitados; no obstante, de los datos disponibles se conoce que amoxicilina-ácido clavulánico, cefalosporinas de segunda y tercera generaciones y fluoroquinolonas son generalmente activas frente a ellas (AU)


Bacteria from the HACEK group (Haemophilus, Aggregatibacter, Cardiobacterium, Eikenella, Kingella), Pasteurella and Capnocytophaga are slow-growing gram-negative bacteria that most frequently cause infections in humans. They are part of the microbiota of the upper respiratory and genitourinary tracts of humans and animals, and can cause infections in any location, although mainly skin and soft tissue infections, as well as bacteraemia and endocarditis. Taxonomic classification is complex and under constant review. These are nutritionally demanding bacteria that require blood and chocolate agar, an aerobic atmosphere, generally CO2-enriched, and 48 h incubation for the development of visible colonies. Phenotypic identification at the species level is complicated and not always possible because it requires multiple substrates that are not normally available in routine laboratories or in automated systems. Application of molecular and proteomic techniques has enabled better identification of these bacteria. Treatment of related infections is hindered by a lack of data on susceptibility to antimicrobial agents. However, evidence suggests that amoxicillin-clavulanic acid, second- and third-generation cephalosporins and fluoroquinolones are generally active against these bacteria (AU)


Assuntos
Humanos , Bactérias Gram-Positivas/isolamento & purificação , Capnocytophaga/isolamento & purificação , Pasteurella/isolamento & purificação , Bacteriemia/microbiologia , Doenças por Vírus Lento/classificação , Doenças por Vírus Lento/microbiologia , Doenças por Vírus Lento/epidemiologia , Microbiota , Combinação Amoxicilina e Clavulanato de Potássio/uso terapêutico , Cefalosporinas/uso terapêutico , Infecções por Haemophilus/microbiologia , Haemophilus/isolamento & purificação , Aggregatibacter/isolamento & purificação , Cardiobacterium/isolamento & purificação , Eikenella/isolamento & purificação , Kingella/isolamento & purificação
7.
Rev. colomb. biotecnol ; 16(1): 34-44, ene.-jun. 2014. ilus, tab
Artigo em Espanhol | LILACS | ID: lil-715296

RESUMO

La purificación de lipopolisacáridos (LPS) o endotoxinas y su caracterización es un aspecto esencial para estudios que buscan aclarar el papel de estas biomoléculas de bacterias Gram negativas presentes en la cavidad oral y su relación con enfermedades locales periodontales y sistémicas. Este estudio implementa una metodología para la extracción, purificación y caracterización de LPS a partir de bacteria completa de Eikenella corrodens 23834 y Porphyromonas gingivalis W83, utilizando técnicas previamente descritas. La extracción cruda de LPS se realizó con fenol-agua caliente; la purificación se realizó con tratamiento enzimático con nucleasas y proteasa, seguido de cromatografía de exclusión por tamaño (Sephacryl S-200 HR) con deoxicolato de sodio como fase móvil. La caracterización de los extractos purificados se realizó por barrido espectrofotométrico, pruebas bioquímicas de electroforesis SDS-PAGE, ensayo Purpald y la prueba cromogénica de LAL. Como control para la identificación y caracterización de los extractos purificados se utilizaron LPS comerciales de Escherichia coli, Salmonella typhimurium, Rodobacter sphaeroides y Porphyromonas gingivalis. La metodología implementada permitió la obtención de LPS de elevada pureza con la identificación de KDO o heptosas, un quimiotipo de LPS-S (liso) para E. corrodens y LPS-SR (semi-rugoso) para P. gingivalis W83. Ambos LPS purificados mostraron capacidad endotóxica a bajas concentraciones. La metodología implementada en este estudio para la purificación y caracterización de LPS a partir de bacteria completa fue eficiente al compararla con los LPS comerciales.


Purification of lipopolysaccharide (LPS) or endotoxins and its characterization is an important aspect for studies aimed at clarify the role of these biomolecules from Gram negative bacteria present in the oral cavity and its relationship with periodontal and systemic diseases. This study describes an extraction, purification and characterization method of LPS from Eikenella corrodens 23834 and Porphyromonas gingivalis W83. LPS extraction was performed by using hot phenol-water; the purification was done with nuclease and protease enzymatic treatment, followed by size-exclusion chromatography (Sephacryl S-200 HR) with sodium deoxycholate as mobile phase. The characterization of the purified extracts was performed by spectrophotometric scanning, SDS-PAGE biochemical tests, Purpald assay and chromogenic LAL test. As control, commercial LPS from Escherichia coli, Salmonella typhimurium, P. gingivalis, and Rodobacter sphaeroides were used. The methodology mentioned above had allowed obtaining high purity LPS by identifying KDO or heptoses, a chemotype S-LPS (smooth) to E. corrodens; SR-LPS (semi-rough) for P. gingivalis W83. Both purified LPS showed endotoxic capacity at low concentrations. The methodology used in this study for purification and characterization of LPS from the whole bacteria was efficient when it was compared with commercial LPS.


Assuntos
Cromatografia , Eikenella , Endotoxinas , Lipopolissacarídeos , Periodontite , Porphyromonas
8.
J Periodontal Res ; 48(1): 30-6, 2013 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-22762355

RESUMO

BACKGROUND AND OBJECTIVE: There is a bidirectional relationship between periodontal disease and type-2 diabetes mellitus (DM). Inflammatory mediators may negatively affect glycemic control, and increased glucose levels and resultant glycation end-products may alter the host response against bacterial infection. However, no agreement has been reached regarding the effect of DM on periodontal subgingival microbiota. Therefore, the purpose of the present study was to compare the subgingival biodiversity in deep periodontal pockets of subjects with chronic periodontitis and either uncontrolled type-2 diabetes or no diabetes using 16S rRNA gene cloning and sequencing. MATERIAL AND METHODS: Twelve subjects with uncontrolled type-2 diabetes (glycated hemoglobin > 8%) and eleven nondiabetic subjects presenting severe and generalized chronic periodontitis were selected. Subgingival biofilm from periodontal pockets > 5 mm were assessed using the 16S rRNA gene cloning and sequencing technique. RESULTS: Significant differences were observed in subgingival microbiota between diabetic and nondiabetic subjects. Diabetic subjects presented higher percentages of total clones of TM7, Aggregatibacter, Neisseria, Gemella, Eikenella, Selenomonas, Actinomyces, Capnocytophaga, Fusobacterium, Veillonella and Streptococcus genera, and lower percentages of Porphyromonas, Filifactor, Eubacterium, Synergistetes, Tannerella and Treponema genera than nondiabetic individuals (p < 0.05). Moreover, some phylotypes, such as Fusobacterium nucleatum, Veillonella parvula, V. dispar and Eikenella corrodens were detected significantly more often in diabetic subjects than in nondiabetic subjects (p < 0.05). CONCLUSION: Subjects with uncontrolled type-2 diabetes and chronic periodontitis presented significant dissimilarities in subgingival biodiversity compared with nondiabetic subjects.


Assuntos
Bactérias/classificação , Biodiversidade , Periodontite Crônica/microbiologia , Diabetes Mellitus Tipo 2/microbiologia , Gengiva/microbiologia , Actinobacillus/isolamento & purificação , Actinomyces/isolamento & purificação , Adulto , Bactérias/isolamento & purificação , Bacteroides/isolamento & purificação , Biofilmes/classificação , Capnocytophaga/isolamento & purificação , Periodontite Crônica/classificação , Diabetes Mellitus Tipo 2/sangue , Eikenella/isolamento & purificação , Eubacterium/isolamento & purificação , Feminino , Fusobacterium/isolamento & purificação , Gemella/isolamento & purificação , Bactérias Anaeróbias Gram-Negativas/isolamento & purificação , Humanos , Masculino , Neisseria/isolamento & purificação , Perda da Inserção Periodontal/microbiologia , Bolsa Periodontal/microbiologia , Porphyromonas/isolamento & purificação , RNA Bacteriano/análise , RNA Ribossômico 16S/análise , Selenomonas/isolamento & purificação , Streptococcus/isolamento & purificação , Treponema/isolamento & purificação , Veillonella/isolamento & purificação
11.
J Am Dent Assoc ; 141(7): 861-6, 2010 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-20592406

RESUMO

BACKGROUND: Necrotizing fasciitis is a rapidly spreading, soft-tissue infection involving the subcutaneous tissues. Necrotizing fasciitis originating from a dental-related source is rare. Practitioners should be aware that this infection could occur in patients who are immunocompromised and in patients who are healthy. Practitioners must treat this disease aggressively with surgical debridement and intensive medical support. CASE DESCRIPTION: The authors present a case report of a man with poorly controlled diabetes mellitus in whom a periapical infection progressed into a maxillofacial space abscess and finally cervical necrotizing fasciitis (CNF). A delay in his initial visit to a dentist was evident. The authors observed a successful outcome in the patient after he underwent several wide surgical debridement procedures, hyperbaric oxygen therapy and a protracted, intensive hospital stay lasting 34 days. CONCLUSIONS: Dentists should suspect that a patient has CNF when maxillofacial cellulitis or an abscess does not respond to conventional therapy. Findings of spreading skin erythema, induration, purple discoloration and anesthesia suggest necrotizing fasciitis. Early computed tomography scans may reveal gas within the deep tissues of the neck, fascial plane involvement or both. CLINICAL IMPLICATIONS: CNF has high morbidity and mortality rates if rapid aggressive therapy is not pursued. Spread of this polymicrobial infection can lead to mediastinitis or cranial base involvement. Mortality is directly proportional to the time to intervention.


Assuntos
Fasciite Necrosante/etiologia , Abscesso Periapical/complicações , Injúria Renal Aguda/etiologia , Injúria Renal Aguda/terapia , Antibacterianos/uso terapêutico , Desbridamento , Diabetes Mellitus Tipo 1/complicações , Eikenella , Enterobacter , Fasciite Necrosante/microbiologia , Fasciite Necrosante/cirurgia , Fasciite Necrosante/terapia , Hidratação , Haemophilus , Humanos , Oxigenação Hiperbárica , Tempo de Internação , Masculino , Pessoa de Meia-Idade , Pescoço , Neisseria , Abscesso Periapical/microbiologia , Streptococcus pyogenes , Fatores de Tempo
12.
Pediatr Emerg Care ; 25(4): 267-8, 2009 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-19369843

RESUMO

Sinusitis can rarely be latent and present directly with intracranial complications. We present the case of an 11-year-old girl who presented with typical features of meningitis. She underwent neuroimaging because of slow improvement and concern for a brain abscess. Despite no history or examination findings suggestive of sinusitis, she was found to have pansinusitis with intracranial extension causing meningitis and epidural abscess.


Assuntos
Transtornos da Consciência/etiologia , Emergências , Abscesso Epidural/diagnóstico , Meningites Bacterianas/diagnóstico , Sinusite/complicações , Infecções por Bacteroidaceae/complicações , Infecções por Bacteroidaceae/tratamento farmacológico , Infecções por Bacteroidaceae/cirurgia , Cefotaxima/administração & dosagem , Cefotaxima/uso terapêutico , Ceftriaxona/administração & dosagem , Ceftriaxona/uso terapêutico , Criança , Terapia Combinada , Diagnóstico por Imagem/métodos , Quimioterapia Combinada , Eikenella/isolamento & purificação , Endoscopia , Abscesso Epidural/tratamento farmacológico , Abscesso Epidural/etiologia , Feminino , Infecções por Fusobacterium/complicações , Infecções por Fusobacterium/tratamento farmacológico , Infecções por Fusobacterium/cirurgia , Infecções por Bactérias Gram-Negativas/complicações , Infecções por Bactérias Gram-Negativas/tratamento farmacológico , Infecções por Bactérias Gram-Negativas/cirurgia , Infecções por Bactérias Gram-Positivas/complicações , Infecções por Bactérias Gram-Positivas/tratamento farmacológico , Infecções por Bactérias Gram-Positivas/cirurgia , Humanos , Meningites Bacterianas/tratamento farmacológico , Meningites Bacterianas/etiologia , Meningites Bacterianas/microbiologia , Metronidazol/administração & dosagem , Metronidazol/uso terapêutico , Procedimentos Cirúrgicos Otorrinolaringológicos , Peptostreptococcus/isolamento & purificação , Prevotella intermedia/isolamento & purificação , Sinusite/tratamento farmacológico , Sinusite/microbiologia , Sinusite/cirurgia , Infecções Estafilocócicas/complicações , Infecções Estafilocócicas/tratamento farmacológico , Infecções Estafilocócicas/cirurgia , Vancomicina/administração & dosagem , Vancomicina/uso terapêutico
13.
J Clin Microbiol ; 44(1): 257-9, 2006 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-16390985

RESUMO

The incidence of and average time to detection for Haemophilus, Actinobacillus, Cardiobacterium, Eikenella, and Kingella (HACEK) bacteria in blood cultures with standard incubation and the utility of extended incubation of blood culture bottles were reviewed at four tertiary care microbiology laboratories. HACEK organisms were isolated from 35 (<0.005%) of 59,203 positive blood cultures. None of 407 blood cultures with extended incubation grew HACEK or other bacteria. Bacteremia from HACEK bacteria is rare, and extended incubation of blood cultures to recover HACEK bacteria is unnecessary.


Assuntos
Actinobacillus/isolamento & purificação , Sangue/microbiologia , Cardiobacterium/isolamento & purificação , Eikenella/isolamento & purificação , Haemophilus/isolamento & purificação , Kingella/isolamento & purificação , Sepse/diagnóstico , Técnicas Bacteriológicas , Estudos Retrospectivos
14.
J Clin Periodontol ; 28(5): 377-88, 2001 May.
Artigo em Inglês | MEDLINE | ID: mdl-11350499

RESUMO

BACKGROUND: The relationship of cigarette smoking to the composition of the subgingival microbiota is not clear. Some studies indicated higher levels of certain species in smokers, while other studies failed to detect differences in the microbiota between subjects with different smoking histories. Thus, the purpose of the present investigation was to examine the prevalence, proportions and levels of the subgingival species in adult subjects who were current, past or never smokers. METHOD: 272 adult subjects ranging in age from 20-86 years with at least 20 teeth were recruited for study. Smoking history was obtained using a questionnaire. Clinical measures were taken at 6 sites per tooth at all teeth excluding third molars at a baseline visit. Subgingival plaque samples were taken from the mesial surface of all teeth excluding third molars in each subject at baseline and assayed individually for counts of 29 subgingival species using checkerboard DNA-DNA hybridization. Subjects were subset according to smoking history into never (n=124), past (n=98) and current smokers (n=50). Uni-variate and multi-variate analyses were used to seek associations between smoking category and the counts, proportions and prevalence of subgingival species. RESULTS: Greater differences were observed for the prevalence (% of sites colonized) of the test species in the 3 smoking groups than were observed for counts or proportions of total counts. Members of the orange and red complexes including E. nodatum, F. nucleatum ss vincentii, P. intermedia, P. micros, P. nigrescens, B. forsythus, P. gingivalis and T. denticola were significantly more prevalent in current smokers than in the other 2 groups. The difference in prevalence between smokers and non-smokers was due to greater colonization at sites with pocket depth <4 mm. Stepwise multiple linear regression analysis indicated that combinations of the prevalence of 5 microbial species and pack years accounted for 44% of the variance for mean pocket depth (p<0.000001), while the prevalence of 3 microbial taxa along with age, pack years, current smoking and gender accounted for 31% of the variance in mean attachment level (p<0.000001). The difference in prevalence between current and never smokers of all members of the red complex and 8 of 12 members of the orange complex was significantly greater in the maxilla than in the mandible. CONCLUSIONS: The major difference between the subgingival microbiota in subjects with different smoking history was in the prevalence of species rather than counts or proportions. The greater extent of colonization in smokers appeared to be due to greater colonization at pocket depths <4 mm. Differences in colonization patterns between current and never smokers were greater in the maxilla than in the mandible.


Assuntos
Bactérias/classificação , Gengiva/microbiologia , Fumar/fisiopatologia , Adulto , Fatores Etários , Idoso , Idoso de 80 Anos ou mais , Análise de Variância , Bactérias/crescimento & desenvolvimento , Bacteroides/crescimento & desenvolvimento , Contagem de Colônia Microbiana , Placa Dentária/microbiologia , Eikenella/crescimento & desenvolvimento , Feminino , Fusobacterium nucleatum/crescimento & desenvolvimento , Humanos , Modelos Lineares , Masculino , Pessoa de Meia-Idade , Análise Multivariada , Hibridização de Ácido Nucleico , Peptostreptococcus/crescimento & desenvolvimento , Bolsa Periodontal/microbiologia , Periodontite/microbiologia , Porphyromonas gingivalis/crescimento & desenvolvimento , Prevotella/crescimento & desenvolvimento , Prevotella intermedia/crescimento & desenvolvimento , Estudos Retrospectivos , Fatores Sexuais , Treponema/crescimento & desenvolvimento
15.
Oral Microbiol Immunol ; 15(2): 103-11, 2000 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-11155173

RESUMO

A diversity of microbial species has been detected in children's oral flora at an early age. To investigate the composition of the subgingival microbiota of different groups of teeth in children with mixed dentition, 40 systemically healthy children, aged 7-8 years, randomly chosen, were examined. Subgingival plaque samples were taken from the mesiobuccal sites of 21, 41, 16 and 36 permanent teeth and 53, 73, 64 and 84 deciduous teeth. The samples were cultured for bacterial isolation anaerobically and in 10% CO2 plus air using selective and nonselective media. Forty-five different microbial species were isolated from both permanent and deciduous teeth. Streptococcus sanguis (79-70%), Streptococcus mitis (66-65%), Prevotella melaninogenica (51-57%), Eikenella corrodens (51-52%), Capnocytophaga gingivalis (46-34%), Capnocytophaga ochracea (45-45%), Actinomyces naeslundii (39-60%) and Prevotella intermedia (42-35%) were among the most frequently detected species in permanent and deciduous teeth respectively. Several suspected periodontal pathogens, such as Porphyromonas gingivalis, Prevotella loescheii, Campylobacter gracilis, Bacteroides forsythus, Campylobacter concisus, Peptostreptococcus micros and Selenomonas sputigena, albeit less frequently detected, were present in the microbiota of these children. The bacterial species Streptococcus constellatus, Peptostreptococcus micros, Pseudoramibacter alactolyticus, E. corrodens and Fusobacterium nucleatum were associated with non-bleeding permanent and deciduous teeth whereas Streptococcus intermedius, C. concisus, P. intermedia and P. loescheii were associated with bleeding.


Assuntos
Gengiva/microbiologia , Dente Decíduo/microbiologia , Dente/microbiologia , Actinomyces/isolamento & purificação , Capnocytophaga/isolamento & purificação , Criança , Eikenella/isolamento & purificação , Feminino , Gengiva/patologia , Hemorragia/microbiologia , Humanos , Masculino , Prevotella/isolamento & purificação , Streptococcus/isolamento & purificação
16.
J Clin Pediatr Dent ; 25(1): 91-4, 2000.
Artigo em Inglês | MEDLINE | ID: mdl-11314361

RESUMO

It is well established that infective endocarditis (IE) involving the HACEK (Hemophilus, Actinobacillus, Cardiobacter, Eikenella, Kingella) group of microbes occurs in patients with congenital heart defects (CHD) and in those with prosthetic grafts. Dental caries and gingival disease have been presumed to be the focus of microbial shedding. The purpose of this study was to determine if children with CHD had a more severe gingival inflammatory condition and harbored the HACEK group of microbes to a greater extent than normal children. Two groups of 12 age and sex matched children were selected for this study. The experimental group consisted of twelve children with CHD, 1-1/2 to 8 years of age. The control group consisted of 12 healthy children 2 to 8 years of age. Each child had a gingival index score recorded as described by Massler. Subgingival cultures were obtained. Gingival samples were cultured for HACEK microbes and total Streptococcus (spp) using standard techniques. Fisher's exact test was performed with significance defined at P < 0.05. Children with CHD had more severe gingival inflammatory index than the control group (P < 0.05). 8/12 CHD patient had Actinobacillus actinomycetemcomitans (A.a.) as compared with 2/12 controls (P < 0.05). Furthermore, all cyanotic CHD patients (4/4) had A.a. whereas, only 2/12 controls did (P < 0.05). 4/12 CHD patients harbored Eikenella corrodens (E.c.) compared to 1/12 controls (N.S.). There was no significant difference in colonization with E.c. or A.a. between cyanotic and acyanotic patients. No significant difference in total Streptococcus (spp) was found between the two groups. This study suggests that children with CHD have a more severe gingival inflammatory index and are colonized with specific HACEK microbes more so than normal children.


Assuntos
Actinobacillus/classificação , Eikenella/classificação , Gengivite/microbiologia , Bacilos Gram-Negativos Anaeróbios Facultativos/classificação , Haemophilus/classificação , Cardiopatias Congênitas/microbiologia , Kingella/classificação , Actinobacillus/crescimento & desenvolvimento , Aggregatibacter actinomycetemcomitans/crescimento & desenvolvimento , Estudos de Casos e Controles , Criança , Pré-Escolar , Contagem de Colônia Microbiana , Cianose/microbiologia , Cárie Dentária/microbiologia , Eikenella/crescimento & desenvolvimento , Feminino , Gengiva/microbiologia , Gengivite/classificação , Bacilos Gram-Negativos Anaeróbios Facultativos/crescimento & desenvolvimento , Haemophilus/crescimento & desenvolvimento , Humanos , Lactente , Kingella/crescimento & desenvolvimento , Masculino , Índice Periodontal , Estatística como Assunto , Streptococcus/classificação , Streptococcus/crescimento & desenvolvimento
18.
Zentralbl Bakteriol ; 288(3): 319-29, 1998 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-9861676

RESUMO

Ten catalase-positive isolates and one catalase-negative isolate that had been assigned to Eikenella corrodens were compared to the nomenclatural type strain regarding selected phenotypic and molecular features and chromosomal deoxyribonucleic acid (DNA) relatedness using the spectrophotometric method. Five catalase-positive human isolates were assigned to the genomic species Eikenella corrodens on the basis of high DNA relatedness levels. Three others, among them strain Chen UB 204, exhibited only moderate degrees of DNA relatedness to the type strain and with each other. Two catalase-positive isolates from dogs were closely interrelated, but yielded only low degrees of DNA binding with Eikenella corrodens and the Eikenella-like human isolates. These findings confirm that the human eikenellas comprise more than one genomic species and that the canine strains represent a distinct taxonomic entity. The differentiation of the strains investigated by conventional phenotypic features, hydrolytic enzyme reactions, and cellular carbohydrate patterns was considered.


Assuntos
Catalase/metabolismo , Eikenella corrodens/classificação , Eikenella/classificação , Animais , Técnicas de Tipagem Bacteriana , DNA Bacteriano/análise , Doenças do Cão/microbiologia , Cães , Eikenella/enzimologia , Eikenella/isolamento & purificação , Eikenella corrodens/enzimologia , Eikenella corrodens/isolamento & purificação , Infecções por Bactérias Gram-Negativas/microbiologia , Infecções por Bactérias Gram-Negativas/veterinária , Humanos , Boca/microbiologia , Hibridização de Ácido Nucleico , Fenótipo
19.
Antonie Van Leeuwenhoek ; 69(4): 371-3, 1996 May.
Artigo em Inglês | MEDLINE | ID: mdl-8836435

RESUMO

Interactions between the 'Streptococcus milleri-group' organisms (SMG; S. intermedius, S. constellatus and S. anginosus) and Eikenella corrodens were investigated. Coaggregation reactions occurred frequently between S. anginosus (83% of strain combinations) or S. constellatus (87%) and E. corrodens isolates, but were infrequent between S. intermedius and E. corrodens (28%). No enhancement of enzyme activities against lipid, phosphate, peptide and saccharide substrates tested were detected with combinations of species in comparison to the species alone. Exponential growth of S. constellatus and S. intermedius, in mixed culture with E. corrodens, occurred within 6h post inoculation, in comparison to 25h without E. corrodens. No growth stimulation of S. anginosus was observed. It is concluded that both coaggregation and growth stimulation occur between E. corrodens and SMG isolates, and may be important mechanisms in the establishment of mixed infections involving these bacteria.


Assuntos
Aderência Bacteriana , Eikenella/metabolismo , Infecções/microbiologia , Infecções/patologia , Streptococcus/metabolismo , Divisão Celular , Meios de Cultura/química , Eikenella/classificação , Eikenella/enzimologia , Metabolismo dos Lipídeos , Peptídeos/metabolismo , Streptococcus/classificação , Streptococcus/enzimologia
20.
Int J Syst Bacteriol ; 43(3): 490-9, 1993 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-8347509

RESUMO

Fourteen human periodontal isolates recovered from a purported Eikenella corrodens-selective medium containing 1 microgram of clindamycin per ml displayed biochemical traits which differed from those described for E. corrodens. These organisms were gram-negative rods which corroded agar. The isolates were oxidase positive and urease, indole, and esculin negative. They differed from E. corrodens in catalase, nitrate reduction, lysine decarboxylase, and ornithine decarboxylase activities. One isolate, strain UB-294, was presumptively identified as Kingella denitrificans. A second isolate, strain UB-204, differed from E. corrodens by being catalase positive and nitrate reduction negative. Twelve isolates, including strain UB-38T (T = type strain), were phenotypically similar to Kingella kingae except that they did not produce acid from maltose and were not beta-hemolytic. Essentially complete (1,480-base) 16S rRNA sequences were determined for strains UB-38T, UB-204, and UB-294 and the type strains of Neisseria animalis, Neisseria canis, Neisseria denitrificans, Neisseria elongata, Neisseria flavescens, Neisseria macaca, and Neisseria polysaccharea. These sequences were compared with the previously published sequences of six other species belonging to the family Neisseriaceae. On the basis of the results of the comparative sequence analysis, UB-294 was confirmed as a K. denitrificans strain, UB-204 was identified as a member of a new species which may belong in the genus Eikenella, and UB-38T was identified as a member of a new species of the genus Kingella, for which we propose the name Kingella oralis [corrected]. Since strain UB-204 was the only representative of a new species, it was not named.(ABSTRACT TRUNCATED AT 250 WORDS)


Assuntos
Placa Dentária/microbiologia , Neisseriaceae/classificação , Proteínas de Bactérias/análise , Técnicas de Tipagem Bacteriana , Sequência de Bases , Eikenella/classificação , Dados de Sequência Molecular , Neisseriaceae/isolamento & purificação , Neisseriaceae/ultraestrutura , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Homologia de Sequência do Ácido Nucleico
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