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1.
Sci Rep ; 10(1): 15354, 2020 09 18.
Artigo em Inglês | MEDLINE | ID: mdl-32948808

RESUMO

Recent studies have shown how intestinal parasites can modulate gut microbiota. This observation is not surprising since the human intestinal lumen, like any other niche, is a battlefield of microbial competition, and Eukaryotes can affect bacterial populations. Intestinal pathogenic protist has been associated with reshaping the microbial community structure; however, the interactions between the colonic bacterial communities and parasites like Blastocystis spp., Entamoeba coli, and Endolimax nana have been poorly studied. In this work, we studied the distal intestinal bacterial microbiota of 49 children attending 7 public daycare centers in Medellin, Colombia, and compared the bacterial microbiota structure in the presence or absence of the protists Blastocystis spp., E. coli, and E. nana. Parasite colonization was associated with an increase in bacterial richness. Moreover, Blastocystis spp. presented a positive relationship with Prevotella, since this bacterium was selectively enriched in children carrying it. Remarkably, the E. coli colonized children showed a microbial profile that was closer to uninfected controls, although some bacterial taxa displayed to be enriched. This is the case for Akkermansia, which showed to be favored in E. coli colonized individuals, while notably reduced in the Blastocystis spp. parasitized group.


Assuntos
Amebíase/microbiologia , Fezes/parasitologia , Microbioma Gastrointestinal/fisiologia , Bactérias/genética , Blastocystis/patogenicidade , Infecções por Blastocystis/microbiologia , Pré-Escolar , Colômbia , Endolimax/patogenicidade , Entamoeba/patogenicidade , Entamebíase/microbiologia , Fezes/microbiologia , Feminino , Microbioma Gastrointestinal/genética , Humanos , Lactente , Enteropatias Parasitárias/microbiologia , Enteropatias Parasitárias/parasitologia , Masculino , Prevotella/genética
2.
Ann Lab Med ; 40(5): 382-389, 2020 09.
Artigo em Inglês | MEDLINE | ID: mdl-32311851

RESUMO

BACKGROUND: Giardia duodenalis is conventionally diagnosed in fecal samples using parasitological methods. However, sensitivity is poor when only a single sample is analyzed, due to intermittent excretion of cysts in feces. Alternatively, the serum antibodies to G. duodenalis can be used for parasite diagnosis and epidemiological studies to determine previous exposure. We compared the rate of G. duodenalis infection between serum anti-Giardia IgG and IgA antibodies and fecal examination in Brazilian children. METHODS: Fecal and serum samples were tested from 287 children at a clinical laboratory and from 187 children at daycare centers. Fecal samples were processed using conventional parasitological methods and coproantigen detection for Giardia diagnosis. Serum samples were tested using an in-house ELISA for detection of anti-Giardia IgG and IgA. RESULTS: G. duodenalis was found in 8.2% (N=39) of the 474 children analyzed. The sensitivity and specificity of ELISA were 80.0% and 90.0% for IgG and 80.0% and 83.3% for IgA, respectively. The total positivity rate of anti-Giardia IgG and IgA in the sera was 13.9% (N=66) and 23.6% (N=112). The agreement between the positivity of specific antibodies and the detection of G. duodenalis in feces was moderate for ELISA-IgG, kappa index (95% CI)=0.543 (0.422-0.664), and mild for ELISA-IgA, kappa index (95% CI)=0.283 (0.162-0.404). Among the children infected with other enteroparasites, 11.6% (N=10) and 24.4% (N=21) showed reactivity to anti-Giardia IgG and to IgA, respectively. This cross-reactivity was more frequent in samples from children infected with Endolimax nana and Entamoeba coli. CONCLUSIONS: The higher frequency of specific antibody reactivity compared with G. duodenalis diagnosis in feces could reflect continuous exposure of children to G. duodenalis infection, resulting in long-lasting immunological memory and/or cross-reactivity with other intestinal amoebas.


Assuntos
Ensaio de Imunoadsorção Enzimática/métodos , Giardia/imunologia , Giardíase/diagnóstico , Imunoglobulina A/sangue , Imunoglobulina G/sangue , Criança , Pré-Escolar , Estudos Transversais , Endolimax/imunologia , Fezes/parasitologia , Feminino , Giardia/isolamento & purificação , Giardia/metabolismo , Giardíase/parasitologia , Humanos , Imunoglobulina A/imunologia , Imunoglobulina G/imunologia , Lactente , Recém-Nascido , Masculino , Sensibilidade e Especificidade
3.
J Infect Dev Ctries ; 14(3): 321-322, 2020 03 31.
Artigo em Inglês | MEDLINE | ID: mdl-32235095

RESUMO

Endolimax nana is a commensal protozoan of the colon. We report a case of chronic urticaria associated with E. nana in a 34-year-old Italian woman. The patient suffered from abdominal pain, diarrhoea and weight loss. The disease appeared after a trip to Vietnam. Laboratory examinations showed mild blood eosinophilia. Three coproparasitological examinations were positive for cysts of E. nana. The patient was successfully treated with two courses of metronidazole (2 g/day for 10 days each). No antihistamines were used. Three coproparasitological examinations, carried out at the end of the therapy, were negative. Follow up (six months) was negative. E. nana can be responsible for very rare cases of abdominal pain, diarrhoea, polyarthritis and urticaria.


Assuntos
Disenteria Amebiana/diagnóstico , Endolimax/isolamento & purificação , Viagem , Dor Abdominal/etiologia , Adulto , Antiprotozoários/administração & dosagem , Antiprotozoários/uso terapêutico , Disenteria Amebiana/complicações , Disenteria Amebiana/tratamento farmacológico , Fezes/parasitologia , Feminino , Humanos , Itália , Metronidazol/administração & dosagem , Metronidazol/uso terapêutico , Urticária/etiologia
4.
ISME J ; 14(2): 609-622, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-31719654

RESUMO

Documenting the natural diversity of eukaryotic organisms in the nonhuman primate (NHP) gut is important for understanding the evolution of the mammalian gut microbiome, its role in digestion, health and disease, and the consequences of anthropogenic change on primate biology and conservation. Despite the ecological significance of gut-associated eukaryotes, little is known about the factors that influence their assembly and diversity in mammals. In this study, we used an 18S rRNA gene fragment metabarcoding approach to assess the eukaryotic assemblage of 62 individuals representing 16 NHP species. We find that cercopithecoids, and especially the cercopithecines, have substantially higher alpha diversity than other NHP groups. Gut-associated protists and nematodes are widespread among NHPs, consistent with their ancient association with NHP hosts. However, we do not find a consistent signal of phylosymbiosis or host-species specificity. Rather, gut eukaryotes are only weakly structured by primate phylogeny with minimal signal from diet, in contrast to previous reports of NHP gut bacteria. The results of this study indicate that gut-associated eukaryotes offer different information than gut-associated bacteria and add to our understanding of the structure of the gut microbiome.


Assuntos
Biodiversidade , Microbioma Gastrointestinal , Metagenômica , Primatas/microbiologia , Primatas/parasitologia , Animais , Animais Selvagens/microbiologia , Animais Selvagens/parasitologia , Blastocisto/classificação , Cercopithecidae/microbiologia , Cercopithecidae/parasitologia , Cilióforos/classificação , Cilióforos/genética , Cilióforos/isolamento & purificação , Dieta , Endolimax/classificação , Endolimax/genética , Endolimax/isolamento & purificação , Entamoeba/classificação , Entamoeba/genética , Eucariotos/classificação , Eucariotos/genética , Eucariotos/isolamento & purificação , Fezes/microbiologia , Fezes/parasitologia , Fungos/classificação , Fungos/genética , Fungos/isolamento & purificação , Hominidae/microbiologia , Hominidae/parasitologia , Especificidade de Hospedeiro , Lemur/microbiologia , Lemur/parasitologia , Nematoides/classificação , Nematoides/genética , Nematoides/isolamento & purificação , Filogenia , Platirrinos/microbiologia , Platirrinos/parasitologia
5.
MedUNAB ; 23(3): 434-440, 26/11/2020.
Artigo em Espanhol | LILACS | ID: biblio-1141195

RESUMO

Introducción. La contaminación enteroparasitaria en autobuses por parte de usuarios infectados con malas conductas sanitarias potencia el riesgo de infección del resto de los pasajeros con el subsecuente peligro del desarrollo de patologías gastrointestinales. Por esto se evaluó la frecuencia de contaminación de autobuses con el fin de aportar datos que son desconocidos en Venezuela e inexistentes en Latinoamérica. Metodología. El trabajo fue descriptivo, transversal, con muestra no probabilística y accidental, donde la obtención de la muestra consistió en frotar el pasamanos de cada autobús (100 vehículos examinados) tres veces con un hisopo impregnado en solución salina isotónica 0.85%, resuspendiendolo en un tubo de ensayo con 10 ml de solución salina. La muestra se centrifugó a 3000 rpm por diez minutos para luego observar el sedimento al microscopio. Resultados. El 16% de las muestras presentaron contaminación, encontrándose solo dos especies de enteroparásitos, Blastocystis spp. (14% del total de muestras examinadas) y Endolimax nana (5%). Discusión. La abundancia del potencial patógeno Blastocystis sp., se relaciona con lo descrito en exámenes de heces de habitantes de Barquisimeto y del estado Lara, demostrando que los usuarios enfermos con malas conductas higiénicas convierten a los autobuses en un foco de infección que debe ser controlado. Conclusión. La falta de aseo constante en unidades de transporte público y la ausencia de conciencia sanitaria de algunos pasajeros infectados facilitan la transmisión de enteroparásitos endémicos potencialmente productores de enfermedades gastrointestinales. Cómo citar: Traviezo, L. Frecuencia de contaminación por enteroparásitos en pasamanos de autobuses de Barquisimeto, Venezuela. MedUNAB. 2020;23(3):434-440 doi: doi.org/10.29375/01237047.3913


Introduction. Enteroparasite contamination by infected users with poor sanitary habits in buses increases other passengers' risk of infection, with the subsequent danger of developing gastrointestinal pathologies. For this reason, bus contamination frequency was evaluated in order to provide data that is unknown in Venezuela and non-existent in Latin America. Methodology. The work was descriptive and cross-sectional, with non-probability and accidental sampling. The sample was obtained by rubbing each bus' handrail (100 examined vehicles) three times with a swab permeated with a 0.85% isotonic saline solution, resuspending it in a test tube with 10 ml of saline solution. The sample was centrifuged at 3,000 rpm for ten minutes to then observe the sediment under a microscope. Results. Sixteen percent of samples were contaminated, observing only two species of enteroparasites, Blastocystis sp. (14% of total examined samples) and Endolimax nana (5%). Discussion. The abundance of the potential pathogen, Blastocystis sp. is related to what has been described in feces exams on the inhabitants of Barquisimeto and the state of Lara, demonstrating that sick users with poor hygiene habits make buses a point of infection that must be controlled. Conclusion. The lack of constant cleaning in public transportation units and the absence of some infected passengers' sanitary awareness facilitates transmitting endemic enteroparasites that could potentially produce gastrointestinal diseases. Cómo citar: Traviezo, L. Frecuencia de contaminación por enteroparásitos en pasamanos de autobuses de Barquisimeto, Venezuela. MedUNAB. 2020;23(3):434-440 doi: doi.org/10.29375/01237047.3913


Introdução. A contaminação enteroparasitária em ônibus por usuários infectados com comportamentos sanitários inadequados aumenta o risco de infecção do resto dos passageiros com o consequente perigo de desenvolvimento de patologias gastrointestinais. Por isso, avaliou-se a frequência de contaminação de ônibus, a fim de fornecer dados até agora desconhecidos na Venezuela e inexistentes na América Latina. Metodologia. O trabalho foi descritivo, transversal, com amostra não-probabilística e acidental, em que a obtenção da amostra consistiu em esfregar o corrimão de cada ônibus (100 veículos examinados) três vezes com um cotonete impregnado em solução salina isotônica 0.85%, ressuspendendo-o em um tubo de ensaio com 10 ml de solução salina. A amostra foi centrifugada a 3,000 rpm por dez minutos para posteriormente observar o sedimento ao microscópio. Resultados. 16% das amostras apresentaram contaminação, sendo encontradas apenas duas espécies de enteroparasitas, Blastocystis sp. (14% do total das amostras examinadas) e Endolimax nana (5%). Discussão. A abundância do potencial patógeno Blastocystis sp., está relacionada ao que foi descrito em exames de fezes de moradores dos estados de Barquisimeto e Lara, mostrando que usuários doentes e com comportamentos sanitários inadequados fazem do ônibus um foco de infecção que deve ser controlado. Conclusão. A falta de limpeza constante nas unidades de transporte público e a falta de conscientização sobre a saúde de alguns passageiros infectados facilitam a transmissão de enteroparasitos endêmicos potencialmente produtoras de doenças gastrointestinais. Cómo citar: Traviezo, L. Frecuencia de contaminación por enteroparásitos en pasamanos de autobuses de Barquisimeto, Venezuela. MedUNAB. 2020;23(3):434-440 doi: doi.org/10.29375/01237047.3913


Assuntos
Parasitos , Venezuela , Blastocystis , Endolimax , Fômites
6.
Biosci Trends ; 13(5): 402-410, 2019 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-31597818

RESUMO

Certain Desulfovibrio sp. (anaerobic sulfate-reducing bacteria) are indigenous to swine cecum and colon, which are also common habitats for parasitic amoebae such as Entamoeba polecki and Entamoeba suis. In this study, we evaluated the growth-promoting effects of D. desulfuricans co-cultured with Escherichia coli (DH5α) and its products [e.g., hydrogen sulfide (H2S) and certain iron-sulfide (FeS) compounds] using Robinson's medium, on the 4 amoeba isolates from swine-Entamoeba polecki subtype (ST)-1, E. polecki ST-3, Entamoeba suis, and Endolimax sp., and, consequently, a continuous culture system for these amoebae was established. However, this novel culture system was required to regulate the excess H2S dissolved in the medium by increasing air space as amoeba isolates thrive only in large air spaces (30-40%). The effects of air space and H2S and FeS compounds on the growth of E. polecki ST-1 (TDP-5) were determined. E. polecki ST-1 (TDP-5) thrived well in culture bottles with an air space of 30-40% (aerobic) (H2S: ~250-400 µmoles/L), but did not grow at all in an air space < 5% (microaerobic) ( H2S:~800 µmoles/L) and in anaerobic vessels (H2S: 20-30 µmoles/L). In both H2S-depleted and FeS compound-depleted conditions, the amoebae sp. could not thrive either. It was hypothesized that an appropriate concentration of H2S and FeS compounds might function as important physiologically active components of electron carriers such as FeS and ferredoxin.


Assuntos
Divisão Celular/efeitos dos fármacos , Desulfovibrio desulfuricans/metabolismo , Endolimax/efeitos dos fármacos , Entamoeba/efeitos dos fármacos , Sulfeto de Hidrogênio/farmacologia , Animais , Endolimax/crescimento & desenvolvimento , Entamoeba/crescimento & desenvolvimento , Escherichia coli/citologia , Sulfeto de Hidrogênio/metabolismo , Suínos
7.
PLoS One ; 14(7): e0218681, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31291262

RESUMO

Rural children are one of the populations that are most vulnerable to gastrointestinal parasite infections. Such diseases decrease the quality of life and result in growth and cognitive delays in the long term. This cross-sectional study was conducted to determine the frequency of intestinal parasite infections among rural schoolchildren in the municipality of Apulo, Colombia. A total of 97 stool samples from children aged between 5 and 15 years were collected and examined via direct light microscopy. Microscopic examination was repeated with sediments obtained using a fecal parasite concentrator, and the Kato-Katz test was performed. Frequency of intestinal parasite infection was 100%. Endolimax nana (77.35%), Blastocystis sp. (71.1%), Giardia intestinalis (39.1%), Entamoeba coli (25.7%), and the Entamoeba histolytica/dispar/moshkovskii complex (9.2%) were the most prevalent protozoa. Trichuris trichiura was the most prevalent helminth (12.3%), followed by Enterobius vermicularis (6.15%) and Ascaris lumbricoides (5.1%). Among the analyzed associated factors, consumption of untreated water increased the risk of acquiring pathogenic intestinal parasites. Finally, because G. intestinalis was the most prevalent pathogenic protozoan, molecular analysis was conducted to establish genetic assemblages and subassemblages of Giardia through sequence-based genotyping of the glutamate dehydrogenase, triose phosphate isomerase, and beta-giardin genes. A total of 14 G. intestinalis-positive samples were genotyped, which revealed the presence of subassemblages AI (n = 1), AII (n = 7), BIII (n = 2), BIV (n = 2), and BIII/BIV (n = 1) as well as a mixed subassemblage AII + BIII (n = 1). Our results indicate that gastrointestinal parasite infections in the tested population were mainly caused by suboptimal water quality. Moreover, molecular typing of G. intestinalis suggested contamination of water by animal- and human-derived cysts.


Assuntos
Água Potável/parasitologia , Fezes/parasitologia , Infecções por Nematoides/epidemiologia , Infecções por Protozoários/epidemiologia , Adolescente , Animais , Ascaris lumbricoides/classificação , Ascaris lumbricoides/isolamento & purificação , Blastocystis/classificação , Blastocystis/isolamento & purificação , Criança , Pré-Escolar , Colômbia/epidemiologia , Estudos Transversais , Endolimax/classificação , Endolimax/isolamento & purificação , Entamoeba/classificação , Entamoeba/isolamento & purificação , Enterobius/classificação , Enterobius/isolamento & purificação , Feminino , Giardia lamblia/classificação , Giardia lamblia/genética , Giardia lamblia/isolamento & purificação , Humanos , Higiene , Masculino , Infecções por Nematoides/parasitologia , Infecções por Nematoides/transmissão , Prevalência , Infecções por Protozoários/parasitologia , Infecções por Protozoários/transmissão , Qualidade de Vida , População Rural , Trichuris/classificação , Trichuris/isolamento & purificação
8.
Parasitology ; 146(6): 821-827, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-30638175

RESUMO

Amoebae are single-celled parasites frequently colonizing human gut. However, few molecular tools are available for accurate identification. Here, we evaluated a panel of polymerase chain reactions (PCRs) targeting Entamoeba histolytica, Entamoeba dispar, Entamoeba coli, Entamoeba hartmanni, Entamoeba polecki, Endolimax nana and Iodamoeba bütschlii. Thirty-six faecal samples (18 containing at least one amoeba species by microscopy and 18 microscopy negative for amoebae) were tested. Real-time PCRs were used for detection and differentiation of E. histolytica and E. dispar. Conventional PCR with Sanger sequencing were applied for detection and differentiation of E. coli, E. hartmanni, E. polecki, E. nana and I. bütschlii. All microscopy results were confirmed by DNA-based methods. However, more samples were positive for single and mixed amoebic species by DNA-based assays than by microscopy (22 vs 18 and 7 vs 1, respectively). DNA sequencing allowed identification of E. coli subtypes (ST1 and ST2), showed low intra-specific variation within E. hartmanni, identified two phylogenetically distinct groups within E. nana, and identified Iodamoeba at the ribosomal lineage level. Taking into account the high intra-genetic diversity within some of the species at the small subunit (SSU) rRNA gene level, amplification of SSU rRNA genes with subsequent sequencing represents a useful method for detecting, differentiating and subtyping intestinal amoebae.


Assuntos
Amebíase/diagnóstico , Archamoebae/isolamento & purificação , Endolimax/isolamento & purificação , Entamoeba histolytica/isolamento & purificação , Fezes/parasitologia , Técnicas de Diagnóstico Molecular/métodos , Archamoebae/classificação , Archamoebae/genética , Doenças Assintomáticas , DNA de Protozoário/química , DNA de Protozoário/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Endolimax/classificação , Endolimax/genética , Entamoeba histolytica/classificação , Entamoeba histolytica/genética , Humanos , Microscopia , Reação em Cadeia da Polimerase/métodos , RNA Ribossômico 18S/genética , Análise de Sequência de DNA/métodos
9.
Acta Parasitol ; 63(4): 819-825, 2018 Dec 19.
Artigo em Inglês | MEDLINE | ID: mdl-30367755

RESUMO

The aim of the research was to determine the prevalence of non-pathogenic protozoa circulating in the human population of Slovakia. We particularly focused on the socially deprived areas with poor sanitation conditions, as they are one of the factors affecting the transmission of these infections. Within this study, 2760 people were coprologically screened for the presence of protozoan cysts. The analyzed group comprised 1173 men and 1587 women from different regions of Slovakia. The total prevalence (2.03%) of non-pathogenic protozoa species was determined. The prevalence of Entamoeba coli was 0.80%, the prevalence of Endolimax nana 0.58%, and the prevalence of Blastocystis hominis was 0.65%. The presence of non-pathogenic protozoa was more frequent in women than that in men, in all age groups. The highest incidence of Entamoeba coli was found in children aged one month - seven years (0.79%), the lowest in the age group of 19-88 years (0.66%). Endolimax nana was most frequent in 8-18 year-olds (0.95%), where the statistical significance was found (p<0.05). The prevalence of Blastocystis hominis by the age group ranged from 0.39 to 0.95%. We did not find any statistical significance (p>0.05) for Entamoeba coli, and similarly for Blastocystis hominis associated with the sex and age. Although the circulation of non-pathogenic protozoa in the human population is far from being limited to the developing countries, their occurrence is also frequent in the population of developed countries. Despite their controversial pathogenicity, they should not be neglected, particularly in the patients with gastrointestinal symptoms.


Assuntos
Gastroenteropatias/epidemiologia , Enteropatias Parasitárias/epidemiologia , Infecções por Protozoários/epidemiologia , Adolescente , Adulto , Distribuição por Idade , Idoso , Idoso de 80 Anos ou mais , Amebíase/epidemiologia , Amebíase/parasitologia , Infecções por Blastocystis/epidemiologia , Infecções por Blastocystis/parasitologia , Blastocystis hominis/isolamento & purificação , Criança , Pré-Escolar , Endolimax/isolamento & purificação , Entamoeba/isolamento & purificação , Entamebíase/epidemiologia , Entamebíase/parasitologia , Fezes/parasitologia , Feminino , Gastroenteropatias/parasitologia , Humanos , Incidência , Lactente , Enteropatias Parasitárias/parasitologia , Masculino , Pessoa de Meia-Idade , Prevalência , Infecções por Protozoários/parasitologia , Distribuição por Sexo , Eslováquia/epidemiologia , Adulto Jovem
10.
Dis Aquat Organ ; 130(3): 235-240, 2018 09 27.
Artigo em Inglês | MEDLINE | ID: mdl-30259875

RESUMO

Systemic amoebiasis of Senegalese sole Solea senegalensis is caused by Endolimax piscium Constenla, Padrós & Palenzuela, 2014 a cryptic parasitic member of the Archamoebae whose epidemiology is yet unknown. To test whether the parasite can be transmitted horizontally, an experimental trial by cohabitation between non-infected and infected fish was designed. Transmission of the parasite from naturally infected to healthy fish was confirmed in the experiment, with the water as the most likely route of infection. Under the conditions of the study, the infection process was remarkably slow, as parasites could be detected by in situ hybridization within the intestinal mucosa of recipient fish only after 17 wk of cohabitation, and none of the new hosts displayed clinical signs of disease. Long prepatent period and the need for additional triggering factors for the development of the clinical condition are suggested. The intestinal mucosa is proposed as the tissue where the amoeba can survive as endocommensal, but also as an invasion route from which the parasite would disperse to other organs.


Assuntos
Amebíase , Endolimax , Doenças dos Peixes , Amebíase/veterinária , Animais , Endolimax/patogenicidade , Doenças dos Peixes/transmissão , Linguados/microbiologia
11.
Parasitol Res ; 117(12): 3715-3723, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30220046

RESUMO

The purpose of the present study was to determine the prevalence of intestinal helminths and protozoa in colorectal cancer (CRC) patients and to evaluate the possible association between the prevalence and CRC pathogenesis. A total of 200 CRC patients and 200 residents of Tashkent, who had no complaints related to the gastrointestinal tract, were examined by triple coproscopy using a concentration method and estimations of protozoan infection intensity. Of the CRC patients tested, 144 were classified as T1-4N0M0 (without metastases) and 56 were classified as T1-4N1-2M0-1 (with metastases). Parasitological examination was performed during CRC diagnosis before and after surgery and chemotherapy. A significantly higher prevalence of Blastocystis sp., Chilomastix mesnili, Jodamoeba butschlii, and Endolimax nana was found in CRC patients than in the control population (p < 0.0001), amounting to 80, 20, 22.5, and 11.5%, respectively. The high prevalence of Blastocystis sp., as well as the patterns of infection intensity, was stable at all stages of examination. The ratio of the number of CRC patients with and without Blastocystis sp. in the T1-4N0M0 and T1-4N1-2M0-1 groups amounted to 3.3 and 7.0, respectively. The ratios for C. mesnili, E. coli, J. butschlii, and E. nana in both groups were 0.2 and 0.2, 0.07 and 0.07, 0.3 and 0.16, and 0.18 and 0.01, respectively. The prevalence of helminths and Giardia lamblia in CRC patients and the control population was not significantly different. Taken together, these data indicate a possible role for Blastocystis sp. in CRC pathogenesis. Diagnosis, treatment, and further observation of patients with Blastocystis sp. are necessary at all stages of CRC, including during diagnosis and before and after surgery and chemotherapy.


Assuntos
Neoplasias Colorretais/patologia , Neoplasias Colorretais/parasitologia , Helmintíase/epidemiologia , Enteropatias Parasitárias/epidemiologia , Intestinos/parasitologia , Infecções por Protozoários/epidemiologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Amoeba/isolamento & purificação , Animais , Blastocystis/isolamento & purificação , Endolimax/isolamento & purificação , Fezes/parasitologia , Feminino , Giardia lamblia/isolamento & purificação , Helmintíase/parasitologia , Helmintos/isolamento & purificação , Humanos , Enteropatias Parasitárias/parasitologia , Masculino , Pessoa de Meia-Idade , Prevalência , Infecções por Protozoários/parasitologia , Retortamonadídeos/isolamento & purificação , Adulto Jovem
12.
Parasitol Res ; 117(11): 3567-3573, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30121754

RESUMO

The aim of this study was to compare formalin-ethyl acetate concentration and FLOTAC Pellet techniques for the diagnosis of intestinal parasites in human stool samples. The anal swab method was used for the specific detection of Enterobius vermicularis. The study was performed in children and youth from Puerto Madryn (South Argentina). A total of 174 individuals were examined using the formalin-ethyl acetate concentration technique (FECT), the FLOTAC Pellet technique with saturated sodium chloride and zinc sulphate flotation solutions and anal swabs. The performance of copromicroscopic techniques was evaluated according to sensitivity, negative predictive value and Kappa index. Overall, 39.1% of the individuals were parasitised. The most prevalent species was Blastocystis sp. (19%) followed by E. vermicularis (17.8%), Giardia lamblia (6.3%), Entamoeba coli (5.7%), Hymenolepis nana and Endolimax nana (1.1%). The FECT was the most sensitive technique for Blastocystis sp., G. lamblia and E. coli infections, whereas FLOTAC Pellet techniques were the most sensitive for H. nana diagnosis. Anal swabs detected the highest percentage of E. vermicularis infection. This was the first time that the FLOTAC Pellet technique was used to detect intestinal parasites in humans. The FECT continues to be a reliable method for detecting protozoa and the FLOTAC Pellet technique gains importance in the diagnosis of helminths. Anal swab test remains the method of choice for the detection of E. vermicularis. However, when comparing techniques, key factors as preservation methods, preservation times and flotation solutions should be taken into account.


Assuntos
Canal Anal/parasitologia , Enteropatias Parasitárias/diagnóstico , Enteropatias Parasitárias/parasitologia , Acetatos/análise , Adolescente , Animais , Argentina , Criança , Pré-Escolar , Estudos Transversais , Endolimax/isolamento & purificação , Entamoeba/isolamento & purificação , Enterobius/isolamento & purificação , Fezes/parasitologia , Feminino , Formaldeído/análise , Giardia/isolamento & purificação , Giardia lamblia/isolamento & purificação , Helmintos/isolamento & purificação , Humanos , Hymenolepis nana/isolamento & purificação , Lactente , Masculino , Parasitos , Prevalência , Sensibilidade e Especificidade
13.
Am J Trop Med Hyg ; 98(3): 763-767, 2018 03.
Artigo em Inglês | MEDLINE | ID: mdl-29363443

RESUMO

Intestinal parasitic infection rate among school-aged children in Thailand has been decreasing. However, certain intestinal parasites remain problematic in some regions. This cross-sectional study was conducted between February and September 2016 in three suburban government primary schools (KK, BR, and HK), Saraburi, Thailand. Stool was collected from 263 asymptomatic subjects (4-15 years old), using simple direct smear, formalin-ether concentration, Boeck and Drbohlav's Locke-Egg-Serum (LES) medium culture, and agar plate culture. A self-administered questionnaire was used to collect data about lifestyle and socioeconomic status. The overall rate of intestinal parasites was 22.1% (15.6% single infection and 6.5% multiple infections). The helminths involving the digestive system found were Strongyloides stercoralis (1.5%) and Opisthorchis viverrini (0.4%). For protozoan infection, the major cause was Blastocystis hominis (17.5%). The other protozoa included Endolimax nana (4.6%), Entamoeba coli (3.4%), Entamoeba histolytica/Entamoeba dispar (1.1%), and Giardia intestinalis (0.8%). The sensitivity for the detection of B. hominis increased with the LES culture technique. The infection rate of each organism was not significantly different among the three schools except for B. hominis which showed the highest prevalence in the HK school (P = 0.001). This was correlated with the questionnaire results in which the HK school showed the highest risk of drinking contaminated water (P = 0.004). The present study emphasized the persistent problems of protozoan infections among suburban school-aged children. Lifestyle was still an important factor for intestinal parasitic infections among suburban school-aged Thai children in this study. Health education as well as routine surveillance was necessary to control the infections.


Assuntos
Amebíase/epidemiologia , Infecções por Blastocystis/epidemiologia , Entamebíase/epidemiologia , Opistorquíase/epidemiologia , Estrongiloidíase/epidemiologia , Adolescente , Amebíase/diagnóstico , Amebíase/parasitologia , Animais , Infecções por Blastocystis/diagnóstico , Infecções por Blastocystis/parasitologia , Blastocystis hominis/isolamento & purificação , Blastocystis hominis/patogenicidade , Criança , Pré-Escolar , Estudos Transversais , Endolimax/isolamento & purificação , Endolimax/patogenicidade , Entamoeba/isolamento & purificação , Entamoeba/patogenicidade , Entamebíase/diagnóstico , Entamebíase/parasitologia , Fezes/parasitologia , Feminino , Humanos , Masculino , Opistorquíase/diagnóstico , Opistorquíase/parasitologia , Opisthorchis/isolamento & purificação , Opisthorchis/patogenicidade , Classe Social , Strongyloides stercoralis/isolamento & purificação , Strongyloides stercoralis/patogenicidade , Estrongiloidíase/diagnóstico , Estrongiloidíase/parasitologia , Inquéritos e Questionários , Tailândia/epidemiologia
14.
Salud(i)ciencia (Impresa) ; 22(7): 625-630, oct.-nov. 2017. tab.
Artigo em Português | LILACS, BINACIS | ID: biblio-1047084

RESUMO

O parasitismo acontece quando há associação de dois seres vivos onde um deles se desenvolve e prejudica de alguma forma o outro ser, chamado hospedeiro (El parasitismo ocurre cuando existe una asociación de dos seres vivos, donde uno de ellos se desarrolla y afecta de algún modo al otro, que se llama huésped). Alguns parasitos como Entamoeba histolytica/Entamoeba dispar, Giardia lamblia, Hymenolepis nana, Taenia solium, Ascaris lumbricoides, Trichuris trichiura e Enterobius vermicularis são transmitidos pela água e/ou (se transmiten por el agua o por) alimentos contaminados e afetam grande parte da população mundial no desempenho (y afectan a una gran parte de la población mundial en el desarrollo) de suas atividades físicas, mentais e sociais, expondo-a a diversas manifestações clínicas que podem gerar elevados níveis (pueden generar altos niveles) de morbidade. Considerando que, aproximadamente, 11% da população brasileira sofrem com algum tipo de deficiência intelectual e/ou múltipla e pouco se tem estudado sobre a ocorrência (y poco se ha estudiado acerca de la existencia) de enteroparasitos nesse grupo populacional, estudos parasitológicos laboratoriais foram realizados em 50 estudantes da Associação de Pais e Amigos dos Excepcionais (APAE) da cidade de São Mateus, Espírito Santo, Brasil. Do total de amostras analisadas 28 (56%) estavam positivas para um ou mais parasitos e comensais, sendo (uno o más parásitos y comensales, siendo) 5 (17.85%) por G. lamblia, 7 (25.00%) por E. histolytica/E. dispar/E. moschkovskii, 11 (39.28%) por Entamoeba coli, 6 (21.43%) por Balantidium coli, 2 (7.14%) por Endolimax nana e 3 (10.7%) por E. vermicularis. Considerando a elevada frequência de indivíduos parasitados torna-se necessário a busca (se hace necesaria la búsqueda) de investimentos em profilaxia e educação higiênico-sanitária, além da realização (además de la realización) constante de exames parasitológicos, garantindo uma melhor (garantizando una mejor) qualidade de vida à população da APAE de São Mateus.


Parasitism occurs when there is an association between two living beings, in which one develops and in some way harms the other being, called host. Some parasites such as Entamoeba histolytica/Entamoeba dispar, Giardia lamblia, Hymenolepis nana, Taenia solium, Ascaris lumbricoides, Trichuris trichiura and Enterobius vermicularis are transmitted by contaminated water and/or food and affect a large number of the world population in their physical, mental and social activities, exposing them to diverse clinical manifestations that can generate high levels of morbidity. Considering that approximately 11% of the Brazilian population suffers from some type of intellectual and/or multiple deficiency and little has been studied about the occurrence of enteroparasites in this population group, laboratory parasitological studies were performed in 50 students of the Associação de Pais e Amigos dos Excepcionais (APAE) of the city of São Mateus, Espírito Santo, Brazil. From the total of the analyzed samples, 28 (56%) were positive for one or more parasites and commensals, of which 5 (17.85%) were positive for G. lamblia, 6 (25.00%) for E. histolytica/E. dispar, 11 (39.28%) for Entamoeba coli, 7 (21.43%) for Balantidium coli, 2 (7.14%) for Endolimax nana and 3 (10.7%) for E. vermicularis. Considering the high prevalence of parasitic infection in this population, it is necessary to seek greater investment in prophylaxis and hygienic-sanitary education, in addition to constantly conducting parasitological examinations that might guarantee a better quality of life for the population of São Mateus APAE.


Assuntos
Doenças Parasitárias , Parasitologia , Balantidíase , Giardia lamblia/parasitologia , Endolimax , Entamoeba/parasitologia , Entamoeba histolytica/parasitologia , Enterobius/parasitologia , Infecções/parasitologia , Enteropatias Parasitárias , Intestinos/parasitologia
15.
Braspen J ; 32(1): 63-67, jan.-mar. 2017.
Artigo em Português | LILACS | ID: biblio-847971

RESUMO

Introdução: A salada de frutas é um alimento saboroso, nutritivo e comercializado pronto para consumo, porém, muitas vezes o seu preparo e manipulação podem ser feitos de forma inadequada, tornando-a, quando contaminada, um veículo para a disseminação de doenças. Objetivo: O presente trabalho teve como objetivo avaliar as características microbiológicas das saladas de frutas comercializadas por ambulantes em Juazeiro do Norte, CE. Método: As amostras foram coletadas aleatoriamente nas ruas do município em estudo e, posteriormente, diluídas em solução salina estéril, filtradas e inoculadas em tubo contendo o meio Brain Heart Infusion e posteriormente em Ágar Sabouraud, Ágar Eosin Methilen Blue e Ágar Salmonella Shigella. Resultados: Das 10 amostras, 60% apresentaram Klebsiella spp. e Escherichia coli, seguido de E. coli, com 20%, Klebsiella spp., com 10%. Os 10% restantes não apresentaram contaminação. Quanto à análise micológica, todas as amostras apresentaram-se contaminadas por fungos. As amostras ainda foram submetidas à técnica de Hoffman Modificado para análise parasitológica, tendo como parasita mais prevalente Endolimax nana, estando presente de forma isolada, em 40% das amostras, e associada com Entamoeba coli em 20%, Entamoeba histolytica em 10%, Giardia spp. em 10%, enquanto 20% não apresentou contaminação parasitária. Conclusão: Os dados demonstraram que a maioria das saladas de frutas analisadas estava contaminadas por microrganismos patogênicos, havendo assim uma necessidade ainda maior de programas de capacitação higiênica e que os órgãos responsáveis disponibilizem produtos para a higienização dos manipuladores e do local onde acontece a manipulação desses alimentos no momento do seu preparo.(AU)


Introduction: A fruit salad is a delicious food, nutritious and sold ready to eat, but often their preparation and handling can be performed improperly, making it, when contaminated, a vehicle for the spread of diseases. Objective: This study aimed to evaluate the microbiological characteristics of fruit salads sold by street vendors in Juazeiro do Norte, CE. Methods: The samples were collected randomly in the city's streets in study and further diluted in sterile saline, filtered, and inoculated into tube containing the medium Brain Heart Infusion Agar Sabouraud and later, Agar Eosin Methilen Blue Agar Salmonella and Shigella. Results: Of the 10 samples, 60% were Klebsiella spp. and Escherichia coli, E. coli followed with 20%, Klebsiella spp. with 10% and the remaining 10% showed no contamination. The mycological analysis, all samples had infected by fungi. The samples were further subjected to Hoffman technique modified for parasitological analysis, with the more prevalent parasitic Endolimax nana, being present separately, in 40% of samples, and is associated with Entamoeba coli 20%, Entamoeba histolytica in 10% Giardia spp. 10%, while 20% showed no parasitic contamination. Conclusion: The data show that most of the analyzed fruit salads were contaminated by pathogenic microorganisms, so there is an even greater need for hygienic training programs and the bodies make available products for the hygiene of food handlers and where happens the handling of these foods in time of its preparation.(AU)


Assuntos
Humanos , Contaminação de Alimentos , Manipulação de Alimentos , Doenças Transmitidas por Alimentos/prevenção & controle , Frutas/microbiologia , Manejo de Espécimes/instrumentação , Endolimax/parasitologia , Entamoeba/parasitologia , Giardia/parasitologia
16.
Korean J Parasitol ; 54(3): 345-8, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-27417092

RESUMO

Although intestinal protozoans are common etiologies of diarrhea, few studies have been conducted in Myanmar. This study planned to investigate the prevalence of Giardia lamblia, Entamoeba coli, Entamoeba histolytica, and Endolimax nana among schoolchildren and their guardians in suburban areas near Yangon, Myanmar. We performed a cross-sectional survey among schoolchildren and their guardians from 7 primary schools in South Dagon and Hlaing Thar Yar districts, Yangon, Myanmar. Stool samples were observed with a microscope after concentration technique and iodine staining. Total 821 stool samples, including 556 from schoolchildren and 265 from guardians, were examined. The median age was 6 years old for schoolchildren and 36 years old for guardians. A 53.1% of the school children and 14.6 % of the guardians were males. The overall prevalence of each intestinal protozoan species was as follows: 3.4% (28/821) for G. lamblia; 3.5% (29/821) for E. coli; 1.2% (10/821) for E. histoytica, and 3.0% for E. nana. This study showed that intestinal protozoans are common in primary schoolchildren and their guardians in suburban areas near Yangon, Myanmar. Health interventions, such as hand washing education, improvement of sanitation, and establishment of water purification systems are urgently needed in this area.


Assuntos
Amebíase/epidemiologia , Endolimax/isolamento & purificação , Entamoeba/isolamento & purificação , Giardia lamblia/isolamento & purificação , Giardíase/epidemiologia , Enteropatias Parasitárias/epidemiologia , Adulto , Amebíase/parasitologia , Criança , Pré-Escolar , Estudos Transversais , Fezes/parasitologia , Feminino , Giardíase/parasitologia , Humanos , Enteropatias Parasitárias/parasitologia , Masculino , Microscopia , Mianmar/epidemiologia , Parasitologia , Prevalência , Instituições Acadêmicas , Estudantes , População Suburbana
17.
J Fish Dis ; 39(12): 1433-1443, 2016 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-27260115

RESUMO

Systemic amoebiasis of sole is caused by Endolimax piscium, a cryptic parasitic archamoeba whose epidemiology and pathogeny are yet unknown. To establish reliable detection methods for this parasite, a battery of molecular diagnostic tools (ISH, PCR and qPCR) were developed and evaluated with a panel of clinical samples from symptomatic diseased fish and from apparently normal animals of different stocks. As there is neither enough background information on the epidemiology of the disease nor a validated reference method, comparison of tests used a composite reference method approach. The ISH technique was the most specific and sensitive in intestine samples and particularly useful as a reference confirmatory method, while the best method in muscle samples was qPCR. Application of the tests to asymptomatic fish demonstrated presence of parasites in a large proportion (>25%) of their intestines, suggesting that this is the point of entry of the amoebae and the initial stage in the development of the disease. The triggering factors that facilitate the breaching of the intestinal barrier by E. piscium, causing granulomatous lesions in other organs and systemic spreading, are not completely understood but our results point to the connective tissue as a preferential target for parasite development and migration.


Assuntos
Amebíase/veterinária , Endolimax/isolamento & purificação , Doenças dos Peixes/diagnóstico , Linguados , Amebíase/diagnóstico , Amebíase/parasitologia , Animais , Doenças dos Peixes/parasitologia , Intestinos/parasitologia , Sensibilidade e Especificidade
18.
Salud(i)ciencia (Impresa) ; 21(8): 814-823, abr. 2016. tab., graf.
Artigo em Espanhol | LILACS, BINACIS | ID: biblio-1116849

RESUMO

Introduction: Various families of small horticultural producers who supply the regional market live in Pereyra Iraola Park. This paper addresses an integral parasitological study designed to assess the sanitary conditions in the place and their impact on human health. Material and methods: Serial human stool and anal swabs (n = 80), canine faeces (n = 8), farm animals (n = 12), and vegetables samples (n = 21) were analyzed. Concentration techniques by flotation and sedimentation were applied. Through semi-structured surveys socio-environmental variables were studied. Results: The study showed that 84% of the human population was parasitized. The highest prevalence corresponded to Blastocystis sp. (41.2%), Enterobius vermicularis (37.5%) and Entamoeba coli (33.7%). Significant associations between parasitic species were found. Trichuris vulpis (37.5%) was the most prevalent species in canines followed by Ancylostoma caninum, Uncinaria stenocephala and Toxocara canis (25%). Farm animals showed Balantidium coli, Trichuris suis, Capillaria sp., Heterakis sp., Trichostrongylus sp., among others. In vegetables, amoeba cysts, coccidia oocysts, strongylid eggs and larvae of free-living nematodes were found. The presence of G. lamblia in the human population was significantly associated with irregular work of the mother, the lack of parental education and the possession of a vegetable garden. Conclusions: The results observed in the population, and species of zoonotic importance in animals and vegetables suggest that these studies should be furthered and control and environmental sanitation measures implemented in order to improve the quality of life of horticulturists and their productive activity


Introducción: En el parque Pereyra Iraola viven familias de pequeños productores hortícolas que abastecen al mercado regional. El presente trabajo aborda un estudio parasitológico integral, con el fin de evaluar las condiciones sanitarias y su impacto en la salud humana. Materiales y métodos: Se analizaron muestras seriadas de heces humanas y escobillado anal (n = 80), heces caninas (n = 8), de animales de cría (n = 12), y muestras de hortalizas (n = 21). Se aplicaron técnicas de concentración por flotación y sedimentación. Mediante encuestas semiestructuradas se relevaron variables socioambientales. Resultados: El 84% de la población humana resultó parasitada. Las mayores prevalencias correspondieron a Blastocystis sp. (41.2%), Enterobius vermicularis (37.5%) y Entamoeba coli (33.7%). Se hallaron asociaciones significativas entre especies parásitas. En perros, la especie más prevalente fue Trichuris vulpis (37.5%), seguida de Ancylostoma caninum, Uncinaria stenocephala y Toxocara canis (25%). En animales de cría se halló Balantidium coli, Trichuris suis, Capillaria sp., Heterakis sp., Trichostrongylus sp., entre otros. En las hortalizas se encontraron quistes de amebas, ooquistes de coccidios, huevos de estrongilídeos y larvas de nematodes de vida libre. La presencia de G. lamblia en la población humana se asoció significativamente con trabajo inestable de la madre, falta de educación paterna y tenencia de huerta. Conclusiones: Los resultados observados en la población y las especies de importancia zoonótica en animales y hortalizas sugieren profundizar estos estudios y llevar adelante medidas de control y saneamiento ambiental, que mejoren la calidad de vida de los horticultores y su actividad productiva


Assuntos
Parasitologia , Argentina , Verduras , Parasitologia de Alimentos , Giardia lamblia , Blastocystis , Endolimax , Entamoeba , Enterobius , Horticultura , Enteropatias Parasitárias
19.
Rev. salud pública ; 18(1): 10-12, ene.-feb. 2016. tab
Artigo em Inglês | LILACS | ID: lil-777025

RESUMO

Objective To determine the frequency of intestinal parasites in children and soil from Turbaco- Bolívar and associated risks factors. Methods Analytical study in which 390 children between 2 and 12 years old from 10 neighborhoods of Turbaco were included, whose legal representatives gave informed consent. Three serial samples of feces and 10 soil samples were processed. Risk factors were determined through an interview. Physicochemical and structural characteristics of soils were also evaluated. Results Parasites were found in 30.5 % of children. 162 parasites were observed; the most frequent protozoan was Endolimax nana (30.3 %) and in terms of helminthes, the most frequent was Ascaris lumbricoides (4.9 %). No statistical association between age or sex and intestinal parasites (p>0.05) or between risk factors and intestinal parasites (p>0.05) was found. Low frequencies of intestinal parasites were encountered in soil samples, being more common Entamoeba spp., Giardia spp., and Ascaris lumbricoides. Neighborhoods of Turbaco had sandy dry soil with low content of ions, low conductivity and low organic matter. Conclusion This study showed a low frequency of intestinal parasites in feces and soils. Despite this, pathogenic parasites were found which can affect the health of the population. Besides this, a high percentage of intestinal parasites that are transmitted through feces were detected indicating fecal contamination and low level of hygiene.(AU)


Objetivo Determinar la frecuencia de parásitos intestinales en niños y suelos de Turbaco -Bolívar y su asociación con factores de riesgo. Métodos Estudio analítico realizado en Turbaco-Bolívar. Fueron Incluidos 390 niños entre 2 y 12 años de 10 barrios, cuyos representantes legales dieron un consentimiento informado. Fueron procesadas 3 muestras seriadas de heces fecales y 10 muestras de suelos. Los factores de riesgo fueron evaluados a través de una entrevista. Además se determinaron las características fisicoquímicas y estructurales de los suelos. Resultados Se encontraron parásitos en 30,5 % de los niños. Se observaron 162 parásitos, siendo Endolimax nana (30,3 %) el protozoo más frecuente y Ascaris lumbricoides (4,9 %) el helminto más frecuente. No se encontró asociación estadística entre la edad, el sexo y los factores de riesgo con la presencia de parásitos intestinales (p>0,05). Se encontró una baja frecuencia de parásitos en las muestras de suelo, siendo más comunes Entamoeba spp., Giardia spp. y Ascaris lumbricoides. Los barrios de Turbaco tuvieron suelo arenoso, seco con bajo contenido de iones, baja conductividad y poca materia orgánica. Conclusión Este estudio determinó una baja frecuencia de parásitos intestinales en heces y suelos. Sin embargo, se encontraron parásitos patógenos que pueden afectar la salud de la población. Se detectó un alto porcentaje de parásitos que son transmitidos a través de las heces indicando contaminación fecal y bajo nivel de higiene.(AU)


Assuntos
Humanos , Pré-Escolar , Criança , Doenças Parasitárias/epidemiologia , Ascaris lumbricoides/parasitologia , Endolimax/parasitologia , Fezes/parasitologia , Métodos de Análise , Epidemiologia Descritiva , Colômbia
20.
Rev Salud Publica (Bogota) ; 18(1): 117-128, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-28453159

RESUMO

Objective To determine the frequency of intestinal parasites in children and soil from Turbaco- Colombia and associated risks factors. Methods Analytical study in which 390 children between 2 and 12 years old from 10 neighborhoods of Turbaco were included, whose legal representatives gave informed consent. Three serial samples of feces and 10 soil samples were processed. Risk factors were determined through an interview. Physicochemical and structural characteristics of soils were also evaluated. Results Parasites were found in 30.5 % of children. 162 parasites were observed; the most frequent protozoan was Endolimax nana (30.3 %) and in terms of helminthes, the most frequent was Ascaris lumbricoides (4.9 %). No statistical association between age or sex and intestinal parasites (p>0.05) or between risk factors and intestinal parasites (p>0.05) was found. Low frequencies of intestinal parasites were encountered in soil samples, being more common Entamoeba spp., Giardia spp., and Ascaris lumbricoides. Neighborhoods of Turbaco had sandy dry soil with low content of ions, low conductivity and low organic matter. Conclusion This study showed a low frequency of intestinal parasites in feces and soils. Despite this, pathogenic parasites were found which can affect the health of the population. Besides this, a high percentage of intestinal parasites that are transmitted through feces were detected indicating fecal contamination and low level of hygiene.


Assuntos
Fezes/parasitologia , Solo/parasitologia , Animais , Ascaris lumbricoides/isolamento & purificação , Criança , Pré-Escolar , Colômbia , Endolimax/isolamento & purificação , Entamoeba/isolamento & purificação , Giardia/isolamento & purificação , Humanos , Lactente , Enteropatias Parasitárias/parasitologia , Fatores de Risco
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