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Previous research shows that family functioning and an individ-uals temperamentalcharacteristics carry significant weight in explaining subjective happiness. However, thelink between these factors is scarcely considered in emerging adulthood. For moreunderstanding, the main aim of our study was to explore the mediating role of negativeaffect on the re-lationship between family functioning and subjective happiness among asample of 347 college students in Spain. Results showed moderate scores in family cohesion and flexibility were positively associated to a young per-son ́s subjective happiness, whilesome extreme scores in those scales showed the opposite pattern. In contrast, negativeaffect was negatively as-sociated to subjective happiness in collegestudents. SEManalyses revealed that negative affect fully mediates the relationship between bothbalanced and unbalanced family functioning and subjective happiness. As regardsdysfunctional family patterns, we found that negative affect fully mediated therelationship between disengaged, enmeshed and chaotic unbalanced scales, andsubjective happiness. However, this mediational pattern failed to replicate in theunbalanced rigid scale. The social and clinical implica-tions of these results on thepromotion of subjective happiness in early adulthood was discussed, taking into accountSpanishculturalbeliefsonfamily functioning and well-being.(AU)
Estudios previos muestran que el funcionamiento familiar y las características temperamentales del individuo tienen un peso importante en la explicación de su felicidad subjetiva. Sin embargo, el vínculo entre estos factores ha sido escasamente estudiado en la adultez emergente. El objetivo de nuestro trabajo fue explorar el papel mediador del afecto negativo en la relación entre funcionamiento familiar y felicidad en una muestra de 347 estudiantes universitarios españoles. Los análisis mostraron que puntuaciones moderadas en cohesión y flexibilidad familiar se asociaron positivamente a la felicidad subjetiva, al contrario que las puntuaciones extremas de dichas escalas. Por el contrario, el afecto negativo se asoció negativamente con la felicidad. Los análisis SEM mostraron que el afecto negativo medió la relación entre funcionamiento familiar (equilibrado y desequilibrado) y felicidad subjetiva. Con respecto a los patrones familiares disfuncionales, encontramos que el afecto negativo medió la relación entre las escalas de desapego, dependencia y caos, y felicidad. Sin embargo, este patrón mediacional no se observó para la escala de rigidez. Se discuten las implicaciones sociales y clínicas que estos resultados tienen en la promoción de la felicidad subjetiva en la adultez temprana, teniendo en cuenta las creencias culturales españolas sobre funcionamiento familiar y bienestar.(AU)
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Humanos , Masculino , Feminino , Adulto Jovem , Afeto , Felicidade , Família , Dependência Psicológica , Espanha , Conflito FamiliarRESUMO
Heart failure impacts patients' quality of life and life expectancy and significantly affects the daily behaviours and feelings of family caregivers. At the end-of-life, the burden for family caregivers depends on their emotional and sentimental involvement, as well as social costs. OBJECTIVES: The aim of this work is to determine whether and how family caregivers' experiences and expectations vary in relation to the places of care and teams involved in heart failure management. METHODS: A systematic literature review was conducted, by screening manuscripts dealing with the experience of Family Care Givers' (FCGs) of patients with Advanced Heart failure. Methods and results were reported following the PRISMA rules. Papers were searched through three databases (PubMed, Scopus and Web of Science). Seven topics were used to synthetize results by reporting qualitative information and quantitative evidence about the experience of FCGs in places of care and with care teams. RESULTS: Thirty-one papers, dealing with the experience of 814 FCGs, were selected for this systematic review. Most manuscripts came from the USA (N = 14) and European countries (N = 13) and were based on qualitative methods. The most common care setting and provider profile combination at the end of life was home care (N = 22) and multiprofessional teams (N = 27). Family caregivers experienced "psychological issues" (48.4%), impact of patients' condition on their life (38,7%) and "worries for the future" (22.6%). Usually, when family caregivers were unprepared for the future, the care setting was the home, and there was a lack of palliative physicians on the team. DISCUSSION: At the end-of-life, the major needs of chronic patients and their relatives are not health related. And, as we observed, non-health needs can be satisfied by improving some key components of the care management process that could be related to care team and setting of care. Our findings can support the design of new policies and strategies.
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Cuidadores , Insuficiência Cardíaca , Humanos , Cuidadores/psicologia , Cuidados Paliativos/métodos , Qualidade de Vida , Motivação , Insuficiência Cardíaca/terapia , Morte , FamíliaRESUMO
This article is part of a series, Supporting Family Caregivers: No Longer Home Alone, published in collaboration with the AARP Public Policy Institute. Results of focus groups, conducted as part of the AARP Public Policy Institute's No Longer Home Alone video project, supported evidence that family caregivers aren't given the information they need to manage the complex care regimens of family members. This series of articles and accompanying videos aims to help nurses provide caregivers with the tools they need to manage their family member's health care at home. This new group of articles provides practical information nurses can share with family caregivers of persons living with pain. To use this series, nurses should read the articles first, so they understand how best to help family caregivers. Then they can refer caregivers to the informational tear sheet-Information for Family Caregivers-and instructional videos, encouraging them to ask questions. For additional information, see Resources for Nurses. Cite this article as: Horgas, A.L., et al. Assessing Pain in Older Adults. Am J Nurs 2022; 122 (12): 42-48.
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Cuidadores , Família , Humanos , Idoso , Grupos FocaisRESUMO
Compared to nurses in acute care settings, home care nurses face unique threats to safety, including unsanitary conditions in homes, dangerous pets, firearms in the home, hostile patients or family members, high-crime neighborhoods, and motor vehicle accidents while traveling between patients. The purpose of this descriptive study was to explore specific personal safety and environmental concerns faced by home care nurses. Seventy-five home care and home hospice nurses completed an anonymous Qualtrics survey. Seventy-eight percent admitted to feeling unsafe during a home visit. Safety threats included unsafe neighborhoods, aggressive dogs, aggressive or drug-seeking family members, patients with mental health concerns, sexual harassment, and most alarmingly feeling threatened by a firearm. Participants also identified environmental concerns such as secondhand smoke and bedbugs, and a high number of musculoskeletal injuries they perceived to be related to their work in home care. Home care is a rapidly growing industry with a critical need to attract and retain workers. Safety training specific to the workers' role should be offered at the time of hire and annually thereafter. Home care nurses should be aware of threats to safety and employ the strategies of preparation, awareness, alertness, and prevention before and during visits.
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Serviços de Assistência Domiciliar , Humanos , Animais , Cães , Família , Inquéritos e Questionários , Gestão da SegurançaAssuntos
Estimulantes do Sistema Nervoso Central , Cardiopatias Congênitas , Metilfenidato , Gravidez , Feminino , Humanos , Metilfenidato/efeitos adversos , Primeiro Trimestre da Gravidez , Estimulantes do Sistema Nervoso Central/efeitos adversos , Cardiopatias Congênitas/induzido quimicamente , FamíliaRESUMO
OBJECTIVES: to analyze the perception of nursing professionals in an intensive care unit in Angola about humanized care and identify resources necessary for its implementation. METHODS: a qualitative, descriptive study conducted with 15 professionals in June-October/2020 in intensive care unit in Angola. The data were collected through semi-structured interviews; analysis based on the collective subject discourse technique. RESULTS: five central ideas emerged: three related to the perception of humanized care ("From integral vision and empathy to a set of actions in all phases of care", "Humanizing is extending care to family members and companions", "Humanized care requires the establishment of a bond of trust and guarantee of individualized care"); and two on the resources necessary for this care ("Need for infrastructure - human and material resources", "Professional training and humanized care are interconnected"). FINAL CONSIDERATIONS: humanized care involves objectivity and subjectivity; it includes family members. An adequate infrastructure can provide it.
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Empatia , Unidades de Terapia Intensiva , Humanos , Pesquisa Qualitativa , Família , AngolaRESUMO
BACKGROUND: Hospital at home (HaH) provides acute healthcare services in patients' homes instead of traditional in-patient care. Research has reported positive outcomes for patients and reduced costs. Although HaH has developed into a global concept, we have little knowledge about the involvement and role of family caregivers (FCs) of adults. The aim of this study was to explore FC involvement and role during HaH treatment as perceived by patients and FCs in a Norwegian healthcare context. METHODS: A qualitative study was carried out among seven patients and nine FCs in Mid-Norway. The data was obtained through fifteen semi-structured interviews; fourteen were performed individually and one as duad interview. The age of the participants varied between 31 and 73 years, and mean age of 57 years. A hermeneutic phenomenological approach was used, and the analysis was performed according to Kvale and Brinkmann's description of interpretation. RESULTS: We identified three main categories and seven subcategories regarding FC involvement and role in HaH: (1) Preparing for something new and unfamiliar, including the subcategories `Lack of involvement in the decision process` and `Information overload affecting caregiver readiness`, (2) Adjusting to a new everyday life at home, including the subcategories `The critical first days at home`, `Coherent care and support in a novel situation`, and `Prior established family roles influencing the new everyday life at home`, (3) FCs` role gradually diminishes and looking back, including the subcategories `A smooth transition to life beyond hospital at home` and `Finding meaning and motivation in providing care`. CONCLUSIONS: FCs played an important role in HaH, although their tasks, involvement and effort varied across different phases during HaH treatment. The study findings contribute to a greater understanding of the dynamic nature of the caregiver experiences during HaH treatment, which can guide healthcare professionals on how they can provide timely and appropriate support to FCs in HaH over time. Such knowledge is important to decrease the risk of caregiver distress during HaH treatment. Further work, such as longitudinal studies, should be done to examine the course of caregiving in HaH over time to correct or support the phases described in this study.
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Cuidadores , Serviços de Assistência Domiciliar , Humanos , Adulto , Pessoa de Meia-Idade , Idoso , Hospitais , Pesquisa Qualitativa , Noruega , FamíliaRESUMO
The patient empowerment movement has highlighted the importance of providing information to the patients to improve care outcome. However, relatives of patients are not yet taken into consideration. This is especially problematic during surgeries since families are often left without real-time information about the trajectory of the patient, inducing worries. Based on this observation we have developed the SMS-Chir solution that connects our surgery service management system with the automatic sending of SMS at key moments in order to inform families about the progression of the surgery. The system has been conceived thanks to the results of a focus group involving four experts. The evaluation was done by monitoring the use of the system over time and by sending questionnaires after intervention. Results analysis shows a limited use of the system but a high satisfaction of the beneficiaries. This study highlights the importance of managerial factors (resistance to change) in order to onboard the necessary stakeholders in the process.
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Participação do Paciente , Pacientes , Humanos , Grupos Focais , Inquéritos e Questionários , FamíliaRESUMO
INTRODUCTION: Transitional-aged youth (TAY) with mental health and/or addictions (MHA) concerns and their families experience significant challenges finding, accessing, and transitioning through needed MHA care. To develop appropriate supports that assist TAY and their families in navigating MHA care, their experiences of transitions in the MHA care system must be better understood. This scoping review identifies and explores the needs, barriers, and facilitators for TAY and their families when transitioning through MHA care. METHODS: This scoping review commenced with a search of five relevant databases. Three research team members were involved in title, abstract, and full-text scanning and data extraction. Sources focusing on TAY anywhere between the ages of 12-29 years and meeting the study objectives were included. Extractions compiled background and narrative information about the nature and extent of the data. Analysis and synthesis of findings involved numerical description of the general information extracted (e.g., numbers of sources by country) and thematic analysis of narrative information extracted (e.g., family involvement in TAY help-seeking). RESULTS: A total of 5894 sources were identified. Following title and abstract scanning, 1037 sources remained for full-text review. A total of 66 sources were extracted. Findings include background information about extracted sources, in addition to five themes that emerged pertaining to barriers and facilitators to access and transitions through care and the needs and roles of TAY and families in supporting help-seeking and care transitions: holistic supports, proactive preparation, empowering TAY and families, collaborative relationships, and systemic considerations. These five themes demonstrate approaches to care that can ensure TAY and families' needs are met, barriers are mitigated, and facilitators are enhanced. CONCLUSION: This review provides essential contextual information regarding TAY with MHA concerns and their families' needs when seeking care. Such findings lend to an enhanced understanding of how MHA programs can support this population's needs, involve family members as appropriate, reduce the barriers experienced, and work to build upon existing facilitators.
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Comportamento Aditivo , Serviços de Saúde Mental , Humanos , Adolescente , Idoso , Criança , Adulto Jovem , Adulto , Saúde Mental , Comportamento Aditivo/terapia , FamíliaRESUMO
Importance: The expansion of genetic and genomic testing in health care has led to recognition that these tests provide personal as well as clinical utility to patients and families. However, available systematic reviews on this topic have not reported the demographic backgrounds of participants in studies of personal utility, leaving generalizability unclear. Objective: To determine the demographic characteristics of participants in studies examining the personal utility of genetic and genomic testing in health care. Evidence Review: For this systematic review, we utilized and updated the results of a highly cited 2017 systematic review on the personal utility of genetics and genomics, which identified relevant articles published between January 1, 2003, and August 4, 2016. We also used the original methods to update this bibliography with literature published subsequently up to January 1, 2022. Studies were screened for eligibility by 2 independent reviewers. Eligible studies reported empirical data on the perspectives of patients, family members, and/or the general public in the US on the personal utility of any type of health-related genetic or genomic test. We utilized a standardized codebook to extract study and participant characteristics. We summarized demographic characteristics descriptively across all studies and by subgroup based on study and participant characteristics. Findings: We included 52 studies with 13â¯251 eligible participants. Sex or gender was the most frequently reported demographic characteristic (48 studies [92.3%]), followed by race and ethnicity (40 studies [76.9%]), education (38 studies [73.1%]), and income (26 studies [50.0%]). Across studies, participants disproportionately were women or female (mean [SD], 70.8% [20.5%]), were White (mean [SD], 76.1% [22.0%]), had a college degree or higher (mean [SD], 64.5% [19.9%]), and reported income above the US median (mean [SD], 67.4% [19.2%]). Examination of subgroups of results by study and participant characteristics evidenced only small shifts in demographic characteristics. Conclusions and Relevance: This systematic review examined the demographic characteristics of individual participants in studies of the personal utility of health-related genetic and genomic testing in the US. The results suggest that participants in these studies were disproportionately White, college-educated women with above-average income. Understanding the perspectives of more diverse individuals regarding the personal utility of genetic and genomic testing may inform barriers to research recruitment and uptake of clinical testing in currently underrepresented populations.
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Etnicidade , Genômica , Humanos , Feminino , Masculino , Escolaridade , Renda , FamíliaRESUMO
BACKGROUND: Maternal and child health (MCH) improvement has been prioritised in resource-constrained countries. This is due to the desire to meet the global sustainable development goals of achieving a maternal mortality rate of 70 per 100000 live births by 2030. The uptake of key maternal and child health services is crucial for reducing maternal and child health mortalities. Community-Based Interventions (CBIs) have been regarded as among the important strategies to improve maternal and child health service uptake. However, a paucity of studies examines the impacts of CBIs and related strategies on maternal and child health. This paper unveils the contribution of CBIs toward improving MCH in Tanzania. METHODS: Convergent mixed method design was employed in this study. Questionnaires were used to examine the trajectory and trend of the selected MCH indicators using the baseline and end-line data for the implemented CBI interventions. Data was also collected through in-depth interviews and focus group discussions, mainly with implementers of the interventions from the community and the implementation research team. The collected quantitative data was analysed using IBM SPSS, while qualitative data was analysed thematically. RESULTS: Antenatal care visits increased by 24% in Kilolo and 18% in Mufindi districts, and postnatal care increased by 14% in Kilolo and 31% in Mufindi districts. Male involvement increased by 5% in Kilolo and 13% in Mufindi districts. The uptake of modern family planning methods increased by 31% and 24% in Kilolo and Mufindi districts, respectively. Furthermore, the study demonstrated improved awareness and knowledge on matters pertaining to MCH services, attitude change amongst healthcare providers, and increased empowerment of women group members. CONCLUSION: Community-Based Interventions through participatory women groups are vital for increasing the uptake of MCH services. However, the success of CBIs depends on the wide array of contextual settings, including the commitment of implementers of the interventions. Thus, CBIs should be strategically designed to enlist the support of the communities and implementers of the interventions.
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Serviços de Saúde da Criança , Serviços de Saúde Comunitária , Gravidez , Criança , Feminino , Humanos , Masculino , Tanzânia , Família , Mortalidade da CriançaRESUMO
This study analyzed the anemia status and change trend of 219 835 pregnant women in eight provinces from 2016 to 2020 in the Maternal and Newborn Health Monitoring Program(MNHMP). The results showed that from 2016 to 2020, the anemia rate of pregnant women in eight provinces was 41.27%, and the rates of mild, moderate and severe anemia were 28.56%, 12.59% and 0.12% respectively; the anemia rates in eastern, central and western regions were 41.87%, 36.09% and 44.63% respectively, and the anemia rates in urban and rural areas were 39.87% and 42.23%. From 2016 to 2020, the anemia rate of pregnant women decreased from 44.93% to 38.22%, with an average annual decline of 3.86% (95%CI:-5.84%, -1.85%). The anemia rate among pregnant women of the eastern region (AAPC=-6.16%, 95%CI:-9.79%, -2.38%) fell faster than that among pregnant women of the central region (AAPC=0.71%, 95%CI:-6.59%, 8.57%) and western region (AAPC=-1.53%, 95%CI:-5.19%, 2.28%). From 2016 to 2020, the moderate anemia rate in pregnant women decreased from 14.98% to 10.74%, with an average annual decline of 8.72% (95%CI:-12.90%, -4.34%), with a statistically significant difference (P<0.05); AAPC for mild and severe anemia in pregnant women was 1.56% (95%CI: 3.44%, 0.36%) and 18.86% (95%CI: 39.88%, 9.52%), respectively, without statistically significant difference (P>0.05).
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Anemia , Gestantes , Recém-Nascido , Feminino , Humanos , Gravidez , Prevalência , Anemia/epidemiologia , China/epidemiologia , Família , População RuralRESUMO
Objective: To identify and assess the effect of community-based Knowledge Translation Strategies (KTS) on maternal, neonatal, and perinatal outcomes. Methods: We conducted systematic searches in Medline, Embase, CENTRAL, CINAHL, PsycInfo, LILACS, Wholis, Web of Science, ERIC, Jstor, and Epistemonikos. We assessed the certainty of the evidence of the studies using the Grading of Recommendations Assessment, Development, and Evaluation (GRADE) framework. Results: We identified seven quantitative and seven qualitative studies. Quantitative findings suggest that there is a possible effect on reducing maternal mortality (RR 0.65; 95% CI, 0.48-0.87; moderate evidence certainty); neonatal mortality (RR 0.79; 95% CI 0.70-0.90; moderate evidence certainty); and perinatal mortality (RR 0.84; 95% CI 0.77-0.91; moderate evidence certainty) in women exposed to KTS compared to those who received conventional interventions or no intervention at all. Analysis of qualitative studies identified elements that allowed to generate benefit effects in improving maternal, neonatal, and perinatal outcomes. Conclusion: The KTS in maternal, neonatal, and perinatal outcomes might encourage the autonomy of communities despite that the certainty of evidence was moderate.
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Mortalidade Infantil , Ciência Translacional Biomédica , Recém-Nascido , Gravidez , Feminino , Humanos , Mortalidade Perinatal , FamíliaRESUMO
The object of the study was senile people, who sought dental orthopedic care in municipal healthcare institutions of the town of Cheboksary, the Chuvash Republic. The sample size was 1 000 people. The obtained quantitative indicators were subjected to statistical processing using parametric and nonparametric statistical methods. The analysis of mounted orthopedic dent prosthetic structures manufactured and installed in elderly and senile patients shows that the choice of material and method of manufacture is largely determined by such a social factor as the average monthly income. This, in turn, largely depends on income-generating employment and the possibility of obtaining additional financial support from family members in which the patient lives. Social factors have a significant impact on the quality of life in elderly and senile people.
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Envelhecimento , Qualidade de Vida , Humanos , Idoso , Família , Atenção à Saúde , EmpregoRESUMO
Problem: Medication for opioid use disorder (MOUD) is recommended for persons with opioid use disorder (OUD) during pregnancy. However, knowledge gaps exist about best practices for management of OUD during pregnancy and these data are needed to guide clinical care. Period Covered: 2014-2021. Description of the System: Established in 2019, the Maternal and Infant Network to Understand Outcomes Associated with Medication for Opioid Use Disorder During Pregnancy (MAT-LINK) is a surveillance network of seven clinical sites in the United States. Boston Medical Center, Kaiser Permanente Northwest, The Ohio State University, and the University of Utah were the initial clinical sites in 2019. In 2021, three clinical sites were added to the network (the University of New Mexico, the University of Rochester, and the University of South Florida). Persons receiving care at the seven clinical sites are diverse in terms of geography, urbanicity, race and ethnicity, insurance coverage, and type of MOUD received. The goal of MAT-LINK is to capture demographic and clinical information about persons with OUD during pregnancy to better understand the effect of MOUD on outcomes and, ultimately, provide information for clinical care and public health interventions for this population. MAT-LINK maintains strict confidentiality through robust information technology architecture. MAT-LINK surveillance methods, population characteristics, and evaluation findings are described in this inaugural surveillance report. This report is the first to describe the system, presenting detailed information on funding, structure, data elements, and methods as well as findings from a surveillance evaluation. The findings presented in this report are limited to selected demographic characteristics of pregnant persons overall and by MOUD treatment status. Clinical and outcome data are not included because data collection and cleaning have not been completed; initial analyses of clinical and outcome data will begin in 2023. Results: The MAT-LINK surveillance network gathered data on 5,541 reported pregnancies with a known pregnancy outcome during 2014-2021 among persons with OUD from seven clinical sites. The mean maternal age was 29.7 (SD = ±5.1) years. By race and ethnicity, 86.3% of pregnant persons were identified as White, 25.4% as Hispanic or Latino, and 5.8% as Black or African American. Among pregnant persons, 81.6% had public insurance, and 84.4% lived in urban areas. Compared with persons not receiving MOUD during pregnancy, those receiving MOUD during pregnancy were more likely to be older and White and to have public insurance. The evaluation of the surveillance system found that the initial four clinical sites were not representative of demographics of the South or Southwest regions of the United States and had low representation from certain racial and ethnic groups compared with the overall U.S. population; however, the addition of three clinical sites in 2021 made the surveillance network more representative. Automated extraction and processing improved the speed of data collection and analysis. The ability to add new clinical sites and variables demonstrated the flexibility of MAT-LINK. Interpretation: MAT-LINK is the first surveillance system to collect comprehensive, longitudinal data on pregnant person-infant dyads with perinatal outcomes associated with MOUD during pregnancy from multiple clinical sites. Analyses of clinical site data demonstrated different sociodemographic characteristics between the MOUD and non-MOUD treatment groups. Public Health Actions: MAT-LINK is a timely and flexible surveillance system with data on approximately 5,500 pregnancies. Ongoing data collection and analyses of these data will provide information to support clinical and public health guidance to improve health outcomes among pregnant persons with OUD and their children.
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Transtornos Relacionados ao Uso de Opioides , Vigilância da População , Adulto , Feminino , Humanos , Lactente , Gravidez , Etnicidade/estatística & dados numéricos , Família , Hispânico ou Latino/estatística & dados numéricos , Transtornos Relacionados ao Uso de Opioides/tratamento farmacológico , Transtornos Relacionados ao Uso de Opioides/epidemiologia , Transtornos Relacionados ao Uso de Opioides/etnologia , Vigilância da População/métodos , Estados Unidos/epidemiologia , Resultado da Gravidez , Adulto Jovem , Negro ou Afro-Americano/estatística & dados numéricos , Brancos/estatística & dados numéricosRESUMO
Evidence has consistently demonstrated that COVID-19 messenger RNA (mRNA) vaccines are safe when given during pregnancy. COVID-19 mRNA vaccines protect pregnant people and their infants who are too young to receive COVID-19 vaccines. Although generally protective, monovalent vaccine effectiveness was lower during SARS-CoV-2 Omicron variant predominance, in part due to changes in the Omicron spike protein. Bivalent vaccines, that combine ancestral strain and Omicron variant, may improve protection against Omicron variants. Everyone, including pregnant people, should stay up to date with recommended COVID-19 vaccines and bivalent booster, when eligible.
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COVID-19 , Complicações Infecciosas na Gravidez , Feminino , Gravidez , Lactente , Humanos , Vacinas contra COVID-19 , COVID-19/epidemiologia , COVID-19/prevenção & controle , SARS-CoV-2 , Família , Complicações Infecciosas na Gravidez/prevenção & controleRESUMO
Kinesin family member 23 (KIF23) serves as a tumor-promoting gene with prognostic values in various tumors. However, the role of KIF23 in esophageal carcinoma (ESCA) progression is largely unknown. The overlapping differentially expressed genes (DEGs) in GSE12452, GSE17351, and GSE20347 datasets were identified via GEO2R tool and Venn diagram software. KIF23 expression was analyzed using GSE12452, GSE17351, and GSE20347 datasets, GEPIA database, and qRT-PCR. Cell proliferation was assessed by CCK-8 and EdU incorporation assays. Gene set enrichment analysis (GSEA) analysis was performed to investigate the pathways associated with the regulatory mechanisms of KIF23 in ESCA. The expression of E-cadherin, vimentin, N-cadherin, and matrix metalloproteinase-9 (MMP-9) and alternation of Wnt/ß-catenin pathway were detected by western blot analysis. We identified two overlapping upregulated DEGs, among which KIF23 was selected for subsequent experiments. KIF23 was overexpressed in ESCA samples and cells, and knockdown of KIF23 retarded cell proliferation in ESCA cells. Besides, KIF23 knockdown suppressed epithelial-mesenchymal transition (EMT) process in ESCA cells, as evidenced by the increase of E-cadherin expression and the reduction of vimentin, N-cadherin, and MMP-9 expression. GSEA analysis suggested that Wnt signaling pathway was the significant pathway related to KIF23. Moreover, we demonstrated that KIF23 silencing inhibited the Wnt/ß-catenin pathway in ESCA cells. Activation of Wnt/ß-catenin pathway by SKL2001 reversed the effects of KIF23 silencing on cell proliferation and EMT in ESCA cells. In conclusion, KIF23 knockdown inhibited the proliferation and EMT in ESCA cells through blockage of Wnt/ß-catenin pathway.
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Carcinoma , Neoplasias Esofágicas , Humanos , beta Catenina/genética , beta Catenina/metabolismo , beta Catenina/farmacologia , Caderinas/genética , Caderinas/metabolismo , Caderinas/farmacologia , Carcinoma/genética , Linhagem Celular Tumoral , Proliferação de Células/genética , Transição Epitelial-Mesenquimal/genética , Neoplasias Esofágicas/genética , Família , Regulação Neoplásica da Expressão Gênica , Cinesinas/genética , Cinesinas/metabolismo , Cinesinas/farmacologia , Metaloproteinase 9 da Matriz/genética , Metaloproteinase 9 da Matriz/metabolismo , Metaloproteinase 9 da Matriz/farmacologia , Vimentina/genética , Vimentina/metabolismo , Vimentina/farmacologia , Via de Sinalização Wnt/genéticaRESUMO
Traumatic events such as hospitalization of family members in the intensive care setting can influence communication interactions between health-care workers and family members not just because of the acute situation but because it brings feelings resulting from past traumatic events to the surface. Providing trauma-informed care to all patients and families in the critical care setting improves communication and results in encounters that are less likely to result in an escalation of negative emotions and outbursts and provides an environment that is conducive to interprofessional communication between families, patients, and health-care workers.
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Cuidados Críticos , Família , Humanos , Família/psicologia , Pacientes , Hospitalização , ComunicaçãoRESUMO
Accompanying death is part of the daily life of intensive care workers. However, far from being a trivial experience, it mobilizes the teams emotionally and requires them to constantly adjust in order to carry out their mission of care for the patient and his or her loved ones. How do nurses and orderlies deal with end-of-life care and the particularities of death in an intensive care unit?