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1.
PLoS One ; 17(6): e0269854, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35737708

RESUMO

Orthohepevirus B, commonly known as avian hepatitis E virus (aHEV), causes big liver and spleen disease (BLS) or hepatitis-splenomegaly syndrome (HSS) in chickens. BLS is an emerging disease among chicken flocks in several countries around the world. In our previous studies, serology and molecular biology screening revealed that chicken flocks are widely affected by aHEV in Poland. The present study, which was conducted between 2019 and 2020, aimed to investigate the prevalence of aHEV in chicken flocks and other poultry, including ducks, geese, and turkeys. A total of 307 flocks were examined. In addition, 29 samples from captive wild birds (western capercaillies, Tetrao urogallus) were analyzed. In all the investigated poultry species, except turkeys, the nucleic acid sequence covering part of the ORF1 gene of the aHEV genome was detected (34/336 samples, 10.1%). The infection rate was found to be the highest in broiler breeder chicken flocks (14/40 samples; 35%). Phylogenetic analysis of partial ORF1 gene, which encodes helicase, revealed that the obtained sequences belonged to genotypes 2 and 4, while one belonged to genotype 3. Genotype 2 was detected for the first time in domestic geese and ducks, and genotype 4 was detected for the first time in Poland. The study demonstrated the presence of aHEV among the investigated western capercaillies, suggesting that this species is susceptible to aHEV infections and biosecurity is therefore required in western capercaillie breeding facilities.


Assuntos
Hepatite Viral Animal , Hepevirus , Doenças das Aves Domésticas , Animais , Galinhas , Patos , Gansos , Hepatite Viral Animal/epidemiologia , Filogenia , Polônia/epidemiologia , Doenças das Aves Domésticas/epidemiologia , Codorniz , Perus
2.
Viruses ; 14(6)2022 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-35746777

RESUMO

A novel gout disease, characterized by visceral urate deposition with high-mortality, with outbreaks in goslings in China since 2016 was caused by a novel goose astrovirus (GoAstV) and resulted in serious economic loss. However, the epidemiology and variation of the GoAstV in goslings in southern China and its evolutionary history as well as the classification of the GoAstV are unclear. In the present study, systematic molecular epidemiology, and phylogenetic analyses of the GoAstV were conducted to address these issues. Our results showed that the GoAstV is widespread in goslings in southern China, and the genomes of six GoAstV strains were obtained. Two amino acid mutations (Y36H and E456D) were identified in capsid proteins in this study, which is the dominant antigen for the GoAstV. In addition, the GoAstV could be divided into two distinct clades, GoAstV-1 and GoAstV-2, and GoAstV-2 is responsible for gout outbreaks in goslings and could be classified into Avastrovirus 3 (AAstV-3), while GoAstV-1 belongs to Avastrovirus 1 (AAstV-1). Moreover, the emergence of GoAstV-2 in geese was estimated to have occurred in January 2010, approximately 12 years ago, while GoAstV-1 emerged earlier than GoAstV-2 and was estimated to have emerged in April 1985 based on Bayesian analysis. The mean evolutionary rate for the GoAstV was also calculated to be approximately 1.42 × 10-3 nucleotide substitutions per site per year. In conclusion, this study provides insight into the epidemiology of the GoAstV in goslings in southern China and is helpful for understanding the origin and evolutionary history as well as the classification of the GoAstV in geese.


Assuntos
Infecções por Astroviridae , Avastrovirus , Gota , Doenças das Aves Domésticas , Animais , Infecções por Astroviridae/epidemiologia , Infecções por Astroviridae/veterinária , Avastrovirus/genética , Teorema de Bayes , China/epidemiologia , Gansos , Gota/epidemiologia , Gota/veterinária , Filogenia , Doenças das Aves Domésticas/epidemiologia
3.
Viruses ; 14(6)2022 Jun 16.
Artigo em Inglês | MEDLINE | ID: mdl-35746790

RESUMO

West Nile virus (WNV) is an emerging infectious pathogen circulating between mosquitoes and birds but also infecting mammals. WNV has become autochthonous in Germany, causing striking mortality rates in avifauna and occasional diseases in humans and horses. We therefore wanted to assess the possible role of free-ranging poultry in the WNV transmission cycle and infected 15 goslings with WNV lineage 2 (German isolate). The geese were monitored daily and sampled regularly to determine viremia, viral shedding, and antibody development by molecular and serological methods. Geese were euthanized at various time points post-infection (pi). All infected geese developed variable degrees of viremia from day 1 to day 10 (maximum) and actively shed virus from days 2 to 7 post-infection. Depending on the time of death, the WN viral genome was detected in all examined tissue samples in at least one individual by RT-qPCR and viable virus was even re-isolated, except for in the liver. Pathomorphological lesions as well as immunohistochemically detectable viral antigens were found mainly in the brain. Furthermore, all of the geese seroconverted 6 days pi at the latest. In conclusion, geese are presumably not functioning as important amplifying hosts but are suitable sentinel animals for WNV surveillance.


Assuntos
Febre do Nilo Ocidental , Vírus do Nilo Ocidental , Animais , Anticorpos Antivirais , Gansos , Cavalos , Mamíferos , Viremia/veterinária , Vírus do Nilo Ocidental/genética
4.
Poult Sci ; 101(7): 101931, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35679667

RESUMO

Due to the demand for modern goose production and the pressure of environmental protection, the rearing systems of geese are changing from traditional waterside rearing to intensive rearing systems such as floor rearing (FR) and cage rearing (CR) systems. However, little is known about the effects of different rearing systems on goose intestinal functions and cecal microbial composition. Therefore, this study aimed to compare intestinal histomorphology and cecal microbial composition differences in geese reared under CR and FR at 270 d of age. Histomorphological analysis showed that the ileal villus height (VH) to crypt depth (CD) ratio was significantly greater in CR than in FR (P < 0.001). Taxonomic analysis showed that the dominant bacteria of cecal microorganisms in both rearing systems were roughly similar, with Bacteroidota, Firmicutes, Fusobacteriota, and Proteobacteria being the dominant phyla while Bacteroides, Fusobacterium, and uncultured_bacterium_o_Bacteroidales being the dominant genera. Differentially abundant taxa between CR and FR were also identified using Linear Discriminant Analysis Effect Size (LEfSe) analysis (P < 0.05, LDA score > 3.5). Megamonas and Anaerobiospirillum were significantly enriched in the CR group at the genus level, while uncultured_bacterium_f_Rikenellaceae and Sutterella were significantly enriched in the FR group. Notably, we found that the relative abundance of uncultured_bacterium_f_Rikenellaceae was significantly negatively correlated with the ileal VH and VH/CD (P < 0.05). The relative abundance of Megamonas and Anaerobiospirillum were significantly negatively correlated with abdominal fat weight and relative abdominal fat weight (P < 0.01), whereas that of Sutterella was significantly positively correlated with abdominal fat weight and relative abdominal fat weight (P < 0.01). Furthermore, PICRUSt2 analysis indicated that the lipid metabolism pathways of cecal microorganisms were lower enriched in CR than in FR. In conclusion, compared with FR, the CR significantly changed goose ileal histomorphological characteristics and cecal microbial composition, thereby affecting goose physiological functions and production performance.


Assuntos
Microbioma Gastrointestinal , Gansos , Animais , Bactérias , Bacteroidetes , Ceco/microbiologia , Galinhas/fisiologia , Gansos/microbiologia , Intestinos
5.
Poult Sci ; 101(7): 101929, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35691050

RESUMO

In recent years, ostrich disease characterized by paralysis and diarrhea has been circulating in some regions of China, causing huge economic losses to the ostrich breeding industry. In our study, clinical samples from diseased ostriches were collected, and only parvovirus was detected. The virus distribution analysis by histopathology and quantitative real-time PCR assays indicated that the virus had a wide range of tissue tropisms. The full-length genome of the ostrich parvovirus (OsPV) was sequenced and comprehensively analyzed. Interestingly, the phylogenetic and alignment results indicated that the OsPV and the goose parvovirus (GPV) form a separate branch. In contrast to GPV strains, OsPV showed 2 new 14 nucleotide deletions in the inverted terminal repeat (ITR) region. Furthermore, recombination analysis indicated that OsPV was a recombination strain between the vaccine strain SYG61v and the virulent strain B strain, with the major parent of OsPV as the SYG61v strain and the minor parent as the B strain. The 14 nucleotide deletions in the ITR region as well as recombination may be some of the reasons for the cross-species transmission of parvovirus from goose to ostrich. The above data will contribute to a better understanding of the molecular biology of the novel OsPV and help to develop the vaccine candidate strain.


Assuntos
Infecções por Parvoviridae , Parvovirus , Doenças das Aves Domésticas , Struthioniformes , Animais , Galinhas , China/epidemiologia , Patos , Gansos , Genômica , Nucleotídeos , Infecções por Parvoviridae/veterinária , Parvovirinae , Parvovirus/genética , Filogenia , Doenças das Aves Domésticas/epidemiologia
6.
In Vivo ; 36(4): 1651-1666, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35738589

RESUMO

BACKGROUND/AIM: The ATP-binding cassette subfamily B member 5 (ABCB5) transporter plays a pivotal role in melanocyte progenitor cell fusion and has been identified as a tumor-initiating cell marker. In this study, we determined ABCB5 expression in normal tissues among various species, i.e., Homo sapiens, Mus musculus (mouse), Rattus norvegicus (rat), Sus scrofa domesticus (pig), Gallus gallus (chicken), Anser anser (goose), Poecilia reticulata (Guppy fish), and Lumbricus terrestris (earthworm), as well as 426 biopsies of different human tumor types (colorectal, cervical, endometrium, vaginal, nasopharyngeal, kidney, breast, colon, prostate, pancreas, lung, gallbladder, bladder, brain, liver, skin, small intestine, testis, tonsil, uterus, thyroid, stomach, esophagus, fallopian, parotid, and ovary). MATERIALS AND METHODS: Using immunohistochemical staining, ABCB5 expression was detected and evaluated in formalin-fixed, paraffin-embedded sections. RESULTS: High ABCB5 expression was found in normal tissues in specialized cells with secretory and excretory functions, chorionic villi of the placenta, hepatocytes, and blood-tissue barrier sites in the brain and testis. Besides, heterogeneous expression of ABCB5 was also observed in many different tumor types derived from breast, endometrium, ovary, uterus, cervix, prostate, lung, brain, colon, liver, nasopharynx, and others. CONCLUSION: The localization of ABCB5 in different normal tissues suggests that this protein has an excretory pumping role for physiological metabolites and xenobiotics. This physiological role highlighted its possible impact on the development of multidrug resistance in tumors. Further studies are required to establish the possible clinical significance of ABCB5 as a predictive marker for drug resistance and as a prognostic marker for patient survival.


Assuntos
Membro 1 da Subfamília B de Cassetes de Ligação de ATP , Células-Tronco Neoplásicas , Subfamília B de Transportador de Cassetes de Ligação de ATP/genética , Membro 1 da Subfamília B de Cassetes de Ligação de ATP/metabolismo , Animais , Biomarcadores/metabolismo , Galinhas , Feminino , Gansos , Humanos , Masculino , Camundongos , Células-Tronco Neoplásicas/metabolismo , Oligoquetos , Gravidez , Ratos , Pele/metabolismo , Suínos
7.
Artigo em Inglês | MEDLINE | ID: mdl-35564785

RESUMO

Functional trait diversity represents ecological differences among species, and the structure of waterbird communities is an important aspect of biodiversity. To understand the effect of meteorological changes on the waterbird functional diversity and provide suggestions for management and conservation, we selected a study area (726 km2) in Liaohe Estuary, located in northeast China. We explored the trends of the waterbird functional diversity changes in response to meteorological factors using fourth corner analysis. Our study demonstrated that temperature was a key factor that impacted waterbird functional diversity in spring, while precipitation had a greater impact in autumn. The population size of goose and duck was positively associated with temperature and negatively with precipitation, while that of the waders (Charadriiformes) showed opposite association trends. Herbivores and species nesting on the bare ground exhibited responses to meteorological factors similar to those of geese and ducks, while benthivores and waterbirds nesting under grass/shrubs exhibited trends similar to those of waterbirds. Waterbirds with smaller bodies, shorter feathers, and lower reproductive rates preferred higher temperatures and less precipitation than other waterbirds. In addition, we observed seasonal variations in waterbird functional diversity. In spring, we should pay attention to waders, herbivores, and waterbirds nesting on the bare ground when the temperature is low. In autumn, waders, benthivores, and omnivores need more attention under extreme precipitation. As the global climate warms in this study area, waterbird functional diversity is expected to decline, and community composition would become simpler, with overlapping niches. Biodiversity management should involve protecting intertidal habitats, supporting benthic macrofaunal communities, preparing bare breeding fields for waterbirds favoring high temperatures to meet their requirements for population increase, and preventing the population decline of geese and ducks, herbivores, and species nesting under grass/shrubs. The findings of our study can aid in developing accurate guidelines for waterbird biodiversity management and conservation.


Assuntos
Conservação dos Recursos Naturais , Áreas Alagadas , Animais , Ecossistema , Estuários , Gansos , Melhoramento Vegetal , Estações do Ano
8.
J Virol Methods ; 306: 114542, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-35577125

RESUMO

Goose astrovirus (GAstV) is a novel pathogen that was discovered in 2018. It has two genotypes, GAstV-1 and GAstV-2, and both can cause visceral gout of goslings and result in significant economic losses. The present work aimed to develop a duplex TaqMan real-time quantitative reverse transcription PCR (RT-qPCR) assay to distinguish the two genotypes. MegAlign software was used to design two pairs of primers and a pair of matched probes based on the open reading frame 2 (ORF2) sequence with the greatest difference between GAstV-1 and GAstV-2, and primer and probe concentrations and annealing temperatures were optimised. Fluorescence signals were obtained for GAstV-1 and GAstV-2 in the FAM and VIC channels, respectively, but no fluorescent signal was observed for other pathogens. The detection limit for GAstV-1 and GAstV-2 was 33.3 and 33.7 DNA copies/µL, respectively. Intra- and inter-assay variability tests revealed excellent reproducibility. Furthermore, the assay detected GAstV-1 and GAstV-2 in allantoic fluids (100% positive) spiked with viruses, and 70 clinical gout gosling samples were examined, of which 11.4% were positive for GAstV-1, 74.3% were positive for GAstV-2%, and 5.7% were positive for mixed infection. In summary, the developed duplex RT-qPCR assay has high specificity, sensitivity, and reproducibility, and can be used in the clinic for detection of GAstV-1 and GAstV-2.


Assuntos
Infecções por Astroviridae , Avastrovirus , Gota , Animais , Infecções por Astroviridae/diagnóstico , Infecções por Astroviridae/veterinária , Avastrovirus/genética , Gansos , Genótipo , Reação em Cadeia da Polimerase em Tempo Real , Reprodutibilidade dos Testes , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Sensibilidade e Especificidade
9.
Poult Sci ; 101(7): 101888, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35550999

RESUMO

Since 2017, outbreaks of gosling astroviruses (GoAstV) causing the major symptoms related to gout in geese have posed a threat to China's poultry industry and caused huge economic losses. In this study, tissue samples from goslings with gout and urate deposition as the main symptoms were taken from 14 goose farms in different regions of China and screened for pathogen infection. The infection rate of GoAstV was 100%, whereas the infection rates of goose parvovirus, reovirus, Tembusu virus, and goose hemorrhagic polyomavirus were 2, 4, 0, and 0%, respectively. In total, 14 GoAstV strains were isolated and their complete genomes were sequenced. Based on the phylogenetic trees, the 14 isolated strains were classified as GoAstV (G-I) and were considered distant from strains belonging to GoAstV (G-II). The multiple sequence alignments indicated a tremendous amount of amino acid mutations in some parts of the encoding proteins of these strains; the main mutations were located in open reading frames (ORFs)-ORF1a and ORF2, such as M533V and F568S in ORF1a and A614T in ORF2. On the other hand, Further, 2 of the 14 GoAstV strains were possibly derived through inter-GoAstV-I recombination. Taken together, these findings indicate that GoAstVs are evolving in a more complex manner and have diverse transmission routes.


Assuntos
Infecções por Astroviridae , Gota , Doenças das Aves Domésticas , Animais , Infecções por Astroviridae/epidemiologia , Infecções por Astroviridae/veterinária , Galinhas/genética , China/epidemiologia , Gansos/genética , Heterogeneidade Genética , Genoma Viral , Gota/epidemiologia , Gota/genética , Gota/veterinária , Filogenia
10.
Poult Sci ; 101(7): 101800, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35580375

RESUMO

Goose astrovirus (GoAstV) is a new Avastrovirus of the genus astrovirus causing gout, hemorrhage, and swellings of kidneys that have affected goslings around the major goose-producing regions in China. The GoAstV is divided into goose astrovirus type 1 (GoAstV-1) and goose astrovirus type 2 (GoAstV-2). Although GoAstV-2 is known to be the causative agent of goose gout, little published information about the relationship between GoAstV-1 and goose gout is unknown. In this study, we investigated the presence of GoAstV-1 in 293 visceral tissue/dead embryos samples with gout on different farms in Jiangxi province, China. A survey result indicated that the mono-infection of GoAstV-1 (32.08%) and co-infection of GoAstV-1 (12.28%) with GoAstV-2 in gout goslings in Jiangxi, China. JXGZ, a GoAstV-1 strain, was effectively isolated from the visceral tissue of gosling gout and serially propagated for more than 25 passages in a goose embryo. The JXGZ strain's whole genome was sequenced and investigated. Phylogenetic analysis of complete genome and capsid protein sequences of JXGZ strain show that it was more closely related to GoAstV-1 strain than GoAstV-2 strain and was grouped within the GoAstV-1 cluster. These findings will aid in the development of efficient diagnostic reagents and possible vaccinations by providing insight into the prevalence and genetic evolution of GoAstV-1 in China.


Assuntos
Infecções por Astroviridae , Avastrovirus , Gota , Doenças das Aves Domésticas , Animais , Infecções por Astroviridae/epidemiologia , Infecções por Astroviridae/veterinária , Avastrovirus/genética , Galinhas , China/epidemiologia , Gansos , Gota/epidemiologia , Gota/veterinária , Filogenia , Doenças das Aves Domésticas/epidemiologia
11.
Am Psychol ; 77(3): 478, 2022 04.
Artigo em Inglês | MEDLINE | ID: mdl-35587404

RESUMO

Reports an error in "How personality and policy predict pandemic behavior: Understanding sheltering-in-place in 54 countries at the onset of COVID-19" by Friedrich M. Götz, Andrés Gvirtz, Adam D. Galinsky and Jon M. Jachimowicz (American Psychologist, 2021[Jan], Vol 76[1], 39-49). In the article "How Personality and Policy Predict Pandemic Behavior: Understanding Sheltering-in-Place in 55 Countries at the Onset of COVID-19," by Friedrich M. Götz, Andrés Gvirtz, Adam D. Galinsky, and Jon M. Jachimowicz (American Psychologist, 2021, Vol. 76, No. 1, pp. 39-49, https://doi.org/10.1037/amp0000740), there were two errors. First, there were translation errors in the Japanese and Korean versions of the Ten Item Personality Inventory (TIPI; Gosling et al., 2003). Second, there was an error in the termination logic that applied to 195 individuals: The skip logic that was meant to automatically move participants to terminate the survey if they selected "no, I would not like to participate" was not working for all participants, and 195 of these participants completed the survey even after selecting this option. To rectify these errors, we (a) recoded the data from the Korean version (in which two items had been accidentally swapped in their presentation order), (b) dropped all participants who completed the Japanese version of the data (which contained an inaccurate translation), and (c) dropped all participants for whom the termination logic did not work properly. Together these exclusions amounted to 0.81% of our sample. When we reran all analyses with the corrected sample of 100,196 participants from 54 countries (i.e., 99.19% of the original sample size), all interpretations, significance levels, and standard errors remained exactly the same. There were only minor changes in a few coefficients in our focal model, and these were rare and very small (Model 3, see Table 1). Among the focal predictors, these are "stringency index" (coefficient changes from .094 to .092) and "extraversion" (coefficient changes from -.025 to -.024). Among the control variables, these are "female" (coefficient changes from .036 to .034), "health" (coefficient changes from -.015 to -.016), "logged confirmed cases (t - 1)" (coefficient changes from -.115 to -.122), "logged confirmed deaths (t - 1)" (coefficient changes from .026 to .027) and "estimated infections in one month" (coefficient changes from .012 to .013). The full set of updated analyses is available in the online supplemental materials: https://doi.org/10.1037/ amp0000740.supp. The online version of this article has been corrected. (The abstract of the original article appeared in record 2020-76208-001.) (PsycInfo Database Record (c) 2022 APA, all rights reserved).


Assuntos
COVID-19 , Animais , Gansos , Humanos , Pandemias , Personalidade , Políticas
12.
Gene ; 834: 146612, 2022 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-35618220

RESUMO

Although graylag geese (A. anser) showed similar plumages of white, grey, and white with grey patches compared to those in swan geese (A. cygnoides), it was believed the substantial molecular mechanism for plumage variations were different. To date, studies on genes responsible for diverse plumages among graylag geese were limited and causal mutations remain unknown. In this study, genomes from 57 individuals belonging to six breeds showing different plumages were sequenced at ∼10X depth. Firstly, the allele frequency differences (AFD) of variants on the scaffold394 (NW_013185915.1) between grey and white goose breeds (A. anser) was calculated and a genomic region between 768,290-779,889 bp was detected to carry candidate variants associated with plumages, including one SNP (g. 775,151G > T, ∼18.6 kb upstream of EDNRB2) found to be fixed in white geese. This region was overlapped with the one detected by the haplotype-based sweep analysis, in which significant signals defined a candidate region of 736,610-820,622 bp on the same scaffold. Results from the transcriptomic data showed that expression levels of EDNRB2 and many other melanogenesis-related genes were significantly decreased among white geese compared to that in grey geese, especially at late embryonic stages (>E15). Modifications at transcriptional levels might result in abnormal melanocyte developments and thus the white plumages when they grow up. In addition, a frameshift mutation (C > -) in exon4 of MLANA gene on scaffold176 (NW_013185876.1) was suggested as the causal mutation for sex-linked dilution phenotype in graylag geese although this requires more demonstration experiments. Together with observed white plumages caused by EDNRB2 mutations in coding regions among swan geese and chicken, our study provided new examples to study the parallel evolution.


Assuntos
Gansos , Genômica , Animais , Sequência de Bases , Gansos/genética , Haplótipos , Mutação
13.
Dev Comp Immunol ; 133: 104435, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-35562079

RESUMO

Interferon regulatory factor (IRF) 3 and IRF7 are the most important nuclear transcription factors regulating type-I interferon (IFN) production in mammals and the IRF3 is missing in birds. Our previous study found that IFR7 is the most important IRF in chickens, however, its functions in geese remain unknown. We cloned goose IRF7 (GoIRF7) and conducted bioinformatics analyses to compare the chromosomal location and protein homology of IRF7 in different species. Overexpression of GoIRF7 in DF-1 cells induced the activation of IFN-ß, and this activation correlated positively with the dosage of transfected plasmids. Overexpression of GoIRF7 in goose embryonic fibroblasts (GEFs) induced the expression of IFNs, proinflammatory cytokines, and IFN-stimulated genes (ISGs); it also inhibited replication of Newcastle disease virus (NDV) and vesicular stomatitis virus (VSV). Our results suggest that GoIRF7 is an important regulator of IFNs, proinflammatory cytokines, and ISGs and plays a role in antiviral innate immunity in geese.


Assuntos
Galinhas , Gansos , Animais , Citocinas , Imunidade Inata/genética , Fator Regulador 7 de Interferon/genética , Mamíferos , Vírus da Doença de Newcastle , Replicação Viral
14.
Reprod Biol ; 22(2): 100640, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35429808

RESUMO

Forkhead box L2 (FOXL2) is a forkhead transcription factor essential for proper reproductive function in females and plays a crucial role in ovarian development in many species of vertebrates. However, little research on goose FOXL2 gene has been conducted. In this study, the cDNA and genomic DNA sequences of goose FOXL2 gene were cloned and characterized. The goose FOXL2 is a single exon gene and contains one open reading frame of 918 bp encoding a protein of 305 amino acids. Bioinformatics analysis displays that the deduced FOXL2 amino acid sequence contains the highly conserved forkhead box domain, which shares greatest similarity to avian species, especially to that of ducks and chicken. RT-qPCR analysis indicates that the FOXL2 mRNA is widely expressed in all examined tissues of fertilized female eggs (28 days), and differentially expressed in female adult (70 days) and laying Zhedong White geese (270 days). Meanwhile, FOXL2 is highly expressed in the hypothalamic-pituitary-ovarian axis, especially in the ovary tissues of the adult and laying geese. Furthermore, one microsatellite (TGTC1415-1418----) and five single nucleotide polymorphisms (A1290G, G1495A, T1554C, T1692A, C1695G and T1697G) were identified in the 3'-untranslated regions. All the information derived from this study could be valuable and facilitate further researches on the function of FOXL2 gene in geese.


Assuntos
Gansos , Perfilação da Expressão Gênica , Sequência de Aminoácidos , Animais , Clonagem Molecular , DNA Complementar/química , Feminino , Gansos/genética , Gansos/metabolismo , Filogenia
15.
Water Res ; 217: 118397, 2022 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-35421690

RESUMO

Migratory geese could influence the microbiological water quality; however, their impacts on pathogen dynamics remain largely unknown. In this study, we analyzed the population dynamics of Campylobacter and Arcobacter group bacteria (AGB) in a freshwater lake in Japan over two years. The bacteria were quantified by using both culture-dependent and -independent methods. The potential sources of these bacteria were examined by a high-throughput flaA sequencing approach. Campylobacter was abundantly detected both by culture-dependent and -independent methods in the lake, especially when migratory geese were present in the lake. High-throughput flaA sequencing suggests that geese were the likely source of Campylobacter in the lake. The viable population of Campylobacter exceeds the concentrations that can potentially cause 10-4 infections per person per year when water is used to grow fresh vegetables. The occurrence of AGB, on the other hand, was not directly related to the population of migratory geese. AGB were not detected in geese fecal samples. Diverse AGB flaA genotypes occurred in the lake over multiple seasons. Our results suggest that AGB likely comprise a part of the indigenous microbial population of the lake and grow in response to high nutrient, warm temperature, and low dissolved oxygen concentrations in the lake. Geese therefore can indirectly impact the AGB population by providing nutrients to cause eutrophication and lower the dissolved oxygen concentration. Since geese travel long-distance and disperse their fecal microbiota and nutrients to wide areas, they may have significant impacts on water quality and public health.


Assuntos
Arcobacter , Campylobacter , Animais , Bactérias/genética , Campylobacter/genética , Gansos/microbiologia , Humanos , Lagos , Oxigênio
16.
Vet Res ; 53(1): 29, 2022 Apr 04.
Artigo em Inglês | MEDLINE | ID: mdl-35379320

RESUMO

Interferon regulatory factors (IRFs) play a key role in many aspects of immune response, and IRF1, IRF3, and IRF7 are positive regulators of IFN induction in mammals. However, IRF3, as the most critical regulatory factor in mammals, is naturally absent in birds, which attracts us to study the functions of other members of the avian IRF family. In the present study, we cloned goose IRF1 (GoIRF1) and conducted a series of bioinformatics analyses to compare the protein homology of GoIRF1 with that of IRF1 in other species. The overexpression of GoIRF1 in DF-1 cells induced the activation of IFN-ß, and this activation is independent of the dosage of the transfected GoIRF1 plasmids. The overexpression of GoIRF1 in goose embryonic fibroblasts (GEFs) induced the expression of IFNs, proinflammatory cytokines, and IFN-stimulated genes (ISGs); it also inhibited the replication of green fluorescent protein (GFP)-tagged Newcastle disease virus (NDV) (NDV-GFP) and GFP-tagged vesicular stomatitis virus (VSV) (VSV-GFP). Our results suggest that GoIRF1 is an important regulator of IFNs, proinflammatory cytokines, and ISGs and plays a role in antiviral innate immunity in geese.


Assuntos
Gansos , Vírus da Doença de Newcastle , Animais , Imunidade Inata/genética , Interferon beta/metabolismo , Mamíferos , Vírus da Doença de Newcastle/metabolismo , Replicação Viral/genética
17.
Theriogenology ; 186: 146-154, 2022 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-35468548

RESUMO

Egg-ceasing is a phenomenon that occurs in most avian species and significantly reduces productivity. Although several factors are reported to regulate the reproduction progress, the underlying molecular mechanism of egg-ceasing remains obscure. Herein, we identified and explored the differentially expressed miRNAs and mRNAs involved in ovarian atrophy via high throughput sequencing. We identified a total of 901 mRNAs and 50 miRNAs that were differentially expressed in egg-laying and atrophic ovaries. Among them, numerous differentially expressed gene (DEG) transcripts and target genes for miRNAs were significantly enriched in Gene Ontology terms such as reproductive processes, cell proliferation, and apoptosis pathways. In addition, an interaction network was constructed by considering target relationships and correlation of the expression levels between ovary development-related genes, miRNAs and pathways. We discovered mRNA and miRNAs transcripts that are candidate regulators of ovary development in egg-ceased geese. Our findings expanded our understanding of the functional of miRNAs in ovarian atrophy and demonstrated that RNA-Seq is a powerful tool for examining the molecular mechanism in regulating egg-ceasing.


Assuntos
Gansos , MicroRNAs , Animais , Atrofia/metabolismo , Atrofia/veterinária , Feminino , Gansos/genética , Gansos/metabolismo , Perfilação da Expressão Gênica/veterinária , MicroRNAs/genética , MicroRNAs/metabolismo , Ovário/metabolismo , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Estações do Ano
18.
Appl Environ Microbiol ; 88(8): e0245721, 2022 04 26.
Artigo em Inglês | MEDLINE | ID: mdl-35389252

RESUMO

We retrospectively investigated 326 samples that were collected from goose farms in Hainan Province, China, in 2017. A total of 33 carbapenem-resistant Klebsiella pneumoniae (CRKP) isolates were identified from 326 samples, and the 33 CRKP isolates were characterized based on whole-genome sequencing (WGS) data from the Illumina and Oxford Nanopore Technologies (ONT) platforms. All of these 33 CRKP isolates possessed blaNDM-5, and a single isolate coharbored mcr-1 and blaNDM-5, while 4 isolates carried multiple virulence and metal tolerance gene clusters. One CRKP strain (CMG-35-2) was selected for long sequence reading. A hybrid plasmid carrying the virulence, resistance, and metal resistance gene in the strain was found. It possessed 2 backbones [IncFIB(K)-IncFII(K)] within a single plasmid that were closely related to K. pneumoniae plasmids from a human-associated habitat in the United States and from a human isolate in Hong Kong. A mouse abdominal infection model indicated that that strain was of the moderate virulence phenotype. This study revealed that K. pneumoniae on goose farms is an important reservoir for blaNDM-5 and these bacteria are represented by a diversity of sequence types. The heterozygous multiple drug resistance genes carried on plasmids highlighted the genetic complexity of CRKP and the urgent need for continued active surveillance. IMPORTANCE CRKP is one of the most important pathogens, which can cause infection not only in humans but also in waterfowl. The discovery of blaNDM-5-producing K. pneumoniae in waterfowl farms in recent years suggests that waterfowl are an important reservoir for blaNDM-5-producing Enterobacteriaceae. However, there are few studies on the spread of blaNDM-5-producing bacteria in waterfowl farms. Our study showed that the IncX3 plasmid carrying blaNDM-5 in goose farms is widely present in K. pneumoniae isolates and a large number of resistance genes are accumulated in it. We found a transferable IncFIB-FII hybrid plasmid that combines virulence, resistance, and metal resistance genes, which allow transfer of these traits between bacteria in different regions. The results of this study contribute to a better understanding of the prevalence and transmission of carbapenem-resistant K. pneumoniae in goose farms.


Assuntos
Antibacterianos , Klebsiella pneumoniae , Animais , Antibacterianos/farmacologia , Carbapenêmicos , Farmacorresistência Bacteriana/genética , Fazendas , Gansos , Camundongos , Estudos Retrospectivos , Virulência/genética , beta-Lactamases/genética
19.
Poult Sci ; 101(6): 101865, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35472739

RESUMO

The aim of this study was to investigate the effects of dietary paddy rice on growth performance, carcass traits, bare skin color, and nutrient digestibility in geese. A total of 300 twenty-eight-day-old male goslings were randomly divided into 5 treatment groups with 6 pens containing 10 goslings each. The geese were raised for 42 d on feed with 0% (control), 13, 26, 39, or 52% dietary paddy rice inclusion. Body weight and feed intake per pen were recorded from the arrival of goslings to the end of the trial. On d 70, two goslings were selected from each pen, one of which was used to measure slaughter performance, meat quality, meat proximate composition, and bare skin color, and one was used to determine nutrient utilization. The results showed that goslings fed a diet containing 26, 39, and 52% paddy rice had a higher final body weight, average daily feed intake, and average daily gain than those in the control group (P < 0.05). The abdominal fat yield in dietary paddy rice groups was higher than that of the control group (P < 0.05). There were no effects on the moisture, protein, and fat contents of breast and thigh muscle among the five treatments (P > 0.05). Compared to the control group, the breast muscle of geese fed the paddy rice had a lower L* value and a higher a* value (P < 0.05). Dietary paddy rice decreased the bill score of geese (P < 0.05). Geese in the paddy rice groups exhibited higher total starch digestibility than geese in the control group (P < 0.05). The digestibility of energy, crude protein, crude fat, crude fiber, NDF, and ADF did not differ between groups (P > 0.05). In conclusion, paddy rice is an excellent energy source in geese diets and could improve the growth performance, breast muscle color, and utilization of total starch but increase the abdominal fat yield and decrease the bill color of geese.


Assuntos
Gansos , Oryza , Ração Animal/análise , Animais , Peso Corporal , Galinhas , Dieta/veterinária , Masculino , Carne/análise , Nutrientes , Pigmentação da Pele , Amido/metabolismo
20.
Arch Virol ; 167(6): 1455-1459, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35469095

RESUMO

Despite their widespread distribution, only a single genotype variant of clade 2.3.4.4b H5N1 influenza viruses has been found so far in Europe. Here, we report the detection of a new highly pathogenic avian influenza H5N1 genotype in geese and ducks from a backyard farm in the Czech Republic. Phylogenetic analysis revealed that the Czech H5N1 virus retained the A/Eurasian_Wigeon/Netherlands/1/2020-like backbone with an altered PB2 segment obtained from co-circulating low-pathogenic avian influenza viruses.


Assuntos
Virus da Influenza A Subtipo H5N1 , Influenza Aviária , Animais , Patos/virologia , Europa (Continente)/epidemiologia , Gansos/virologia , Genótipo , Virus da Influenza A Subtipo H5N1/genética , Influenza Aviária/epidemiologia , Influenza Aviária/virologia , Filogenia
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