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1.
Int J Mol Sci ; 23(12)2022 Jun 12.
Artigo em Inglês | MEDLINE | ID: mdl-35742997

RESUMO

Cell fusion (fusogenesis) occurs in natural and pathological conditions in prokaryotes and eukaryotes. Cells of monocyte-macrophage lineage are highly fusogenic. They create syncytial multinucleated giant cells (MGCs) such as osteoclasts (OCs), MGCs associated with the areas of infection/inflammation, and foreign body-induced giant cells (FBGCs). The fusion of monocytes/macrophages with tumor cells may promote cancer metastasis. We describe types and examples of monocyte-macrophage lineage cell fusion and the role of actin-based structures in cell fusion.


Assuntos
Células Gigantes de Corpo Estranho , Monócitos , Diferenciação Celular , Fusão Celular , Células Gigantes/patologia , Células Gigantes de Corpo Estranho/metabolismo , Células Gigantes de Corpo Estranho/patologia , Monócitos/metabolismo , Osteoclastos/metabolismo
2.
J Cutan Pathol ; 48(6): 781-784, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33474744

RESUMO

Hydrophilic polymer-coated devices have been increasingly utilized for various endovascular procedures, however not been without adverse effects. We report two cases of subacute cutaneous lesions on the neck encountered in our dermatology clinic. Histopathologic findings were significant for a nodular aggregate of epithelioid histiocytes and lymphocytes with numerous foreign body giant cells in the dermis. The granulomatous infiltrate was associated with an amorphous basophilic non-polarizable material. Further chart review reveals both patients receiving a central venous procedure in the past, thus attributing the hydrophilic polymers as the likely source of the foreign material found at the insertion site. Our cases contrast to the more commonly reported distal embolization by these hydrophilic polymer layers. We suspect the incidence of retained hydrophilic polymer at the site of prior endovascular procedures may be underreported in the literature with the more inconspicuous presentations. Therefore, retained foreign material should be considered by both treating physicians and dermatopathologists in presenting cases of lesions that occur at common sites of endovascular procedures.


Assuntos
Procedimentos Endovasculares/efeitos adversos , Reação a Corpo Estranho/patologia , Células Gigantes de Corpo Estranho/patologia , Polímeros/efeitos adversos , Idoso , Idoso de 80 Anos ou mais , Biópsia , Procedimentos Endovasculares/instrumentação , Feminino , Reação a Corpo Estranho/diagnóstico , Reação a Corpo Estranho/etiologia , Neoplasias de Cabeça e Pescoço/patologia , Humanos , Doença Iatrogênica/epidemiologia
3.
J Biol Chem ; 296: 100129, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33262217

RESUMO

Multinucleated giant cells are formed by the fusion of macrophages and are a characteristic feature in numerous pathophysiological conditions including the foreign body response (FBR). Foreign body giant cells (FBGCs) are inflammatory and destructive multinucleated macrophages and may cause damage and/or rejection of implants. However, while these features of FBGCs are well established, the molecular mechanisms underlying their formation remain elusive. Improved understanding of the molecular mechanisms underlying the formation of FBGCs may permit the development of novel implants that eliminate or reduce the FBR. Our previous study showed that transient receptor potential vanilloid 4 (TRPV4), a mechanosensitive ion channel/receptor, is required for FBGC formation and FBR to biomaterials. Here, we have determined that (a) TRPV4 is directly involved in fusogenic cytokine (interleukin-4 plus granulocyte macrophage-colony stimulating factor)-induced activation of Rac1, in bone marrow-derived macrophages; (b) TRPV4 directly interacts with Rac1, and their interaction is further augmented in the presence of fusogenic cytokines; (c) TRPV4-dependent activation of Rac1 is essential for the augmentation of intracellular stiffness and regulation of cytoskeletal remodeling; and (d) TRPV4-Rac1 signaling axis is critical in fusogenic cytokine-induced FBGC formation. Together, these data suggest a novel mechanism whereby a functional interaction between TRPV4 and Rac1 leads to cytoskeletal remodeling and intracellular stiffness generation to modulate FBGC formation.


Assuntos
Células Gigantes de Corpo Estranho/metabolismo , Células Gigantes/metabolismo , Macrófagos/metabolismo , Neuropeptídeos/metabolismo , Canais de Cátion TRPV/metabolismo , Proteínas rac1 de Ligação ao GTP/metabolismo , Animais , Fusão Celular , Células Cultivadas , Modelos Animais de Doenças , Células Gigantes/patologia , Células Gigantes de Corpo Estranho/patologia , Macrófagos/patologia , Mecanotransdução Celular , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Neuropeptídeos/genética , Transdução de Sinais , Canais de Cátion TRPV/genética , Proteínas rac1 de Ligação ao GTP/genética
6.
Acta Biomater ; 109: 95-108, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-32268238

RESUMO

In order to elicit a desired barrier function in guided bone regeneration (GBR) or guided tissue regeneration (GTR), a barrier membrane has to maintain its integrity for a certain period of time to guarantee the regeneration of target tissue. Due to the complexity and variety of clinical conditions, the healing time required for tissue regeneration varies from one case to another, which implies the need for tailoring the barrier membranes to diverse conditions via manipulating their degradation property. As a "non-self" biomaterial, a barrier membrane will inevitably trigger host-membrane immune response after implantation, which entails the activation of phagocytic cells. In the degradation process of a barrier membrane, the cell-mediated degradation may play a more vital role than enzymatic and physicochemical dissolution; however, limited studies have been carried out on this topic. In this context, we investigated the cell-mediated degradation and illustrated the possible key cells and mediators for immunomodulation via in vivo and in vitro studies. We discovered that IL-13, a key cytokine mainly released by T helper 2 cells (Th2), induced the formation of foreign body giant cells (FBGCs), thus resulting in membrane degradation. Neutralizing IL-13 could suppress membrane degradation and formation of FBGC. The contributions of this study are (1) unveiling the immune mechanisms underlying the cell-mediated collagen membrane degradation; (2) allowing the formation of an "immunodegradation" strategy to develop an "immune-smart" barrier membrane to manipulate its degradation; (3) providing the key regulatory immune cells and cytokines for the immunomodulation target in collagen membrane degradation. STATEMENT OF SIGNIFICANCE: The significance of this research includes.


Assuntos
Colágeno/metabolismo , Imunidade Celular/efeitos dos fármacos , Fatores Imunológicos/farmacologia , Interleucina-13/metabolismo , Membranas Artificiais , Receptores Tipo II de Interleucina-4/metabolismo , Implantes Absorvíveis , Animais , Colágeno/química , Colágeno/imunologia , Células Gigantes de Corpo Estranho/imunologia , Células Gigantes de Corpo Estranho/metabolismo , Interleucina-13/antagonistas & inibidores , Leucócitos Mononucleares/imunologia , Leucócitos Mononucleares/metabolismo , Masculino , Ratos Wistar , Suínos , Células Th2/metabolismo
8.
Biomedica ; 39(Supl. 2): 26-31, 2019 08 01.
Artigo em Inglês, Espanhol | MEDLINE | ID: mdl-31529831

RESUMO

Patients with lepromatous leprosy that have received treatment for many years usually get follow up biopsies for persistent skin lesions or positive bacilloscopy even if the values are lower than in the initial bacilloscopy. We report the case of a 48-year old woman with long-standing lepromatous leprosy of 15 years of evolution, with a bacterial index of 4 in the direct smear and the initial skin biopsy. The patient was treated with multidrug therapy for 32 months although the treatment recommended by the World Health Organization (WHO) is only for 12 months. A skin biopsy was taken to determine if there was an active disease. We observed a diffuse dermal inflammation with numerous foreign body giant cells and vacuolated macrophages (Virchow´s cells). These cells contained granular acid-fast material that was also positive with immunohistochemistry for BCG. There were fragmented bacilli and the BI was 2. These cells were also strongly positive for CD68. The biopsy was interpreted as a residual form of lepromatous leprosy that did not require further multidrug therapy. We have observed similar histological profiles in several cases. The lack of clinical data makes it a histological challenge. The accumulation of lipids in these giant cells is due to bacillary destruction and fusion of vacuolated macrophages. We discuss here the role of bacillary and host lipids in the pathogenesis of lepromatous leprosy. We concluded that there was no need to extend the 12-month multidrug therapy recommended by WHO.


Los pacientes con lepra lepromatosa (LL) que han recibido tratamiento durante años, usualmente tienen seguimiento con biopsias de piel para lesiones persistentes o con baciloscopia positiva, con valores menores a los iniciales. Presentamos una mujer de 48 años con LL de 15 años de evolución, con índice bacilar (IB) 4 en el extendido directo y en la biopsia, que recibió terapia multidroga durante 32 meses, aunque el tratamiento recomendado por la Organización mundial de la salud (OMS) es de 12 meses. Se tomó una biopsia de piel para determinar si la enfermedad estaba activa. Se observó inflamación dérmica difusa con numerosas células gigantes tipo cuerpo extraño y macrófagos vacuolados (células de Virchow). Estas células, CD68 positivas, contenían material granular ácido-alcohol resistente, positivo con inmunohistoquímica para BCG. Se encontraron bacilos fragmentados y el IB fue de 2. Se interpretó como una forma residual de LL y que la paciente no requería MDT adicional. Este perfil histológico lo hemos observado en casos similares. Sin datos clínicos estas biopsias son un reto diagnóstico. La acumulación de lípidos en estas células gigantes se debe a la destrucción bacilar y a la fusión de macrófagos vacuolados. Revisamos el papel de los lípidos del bacilo y del huésped en la patogénesis de la LL. En estos casos no es necesario extender los 12 meses de MDT recomendados por la OMS. En el seguimiento de los pacientes se recomienda contar con los hallazgos clínicos, la baciloscopia, la biopsia anual de piel y los títulos IgM anti-glicolípido fenólico.


Assuntos
Células Espumosas/patologia , Células Gigantes de Corpo Estranho/patologia , Hanseníase Virchowiana/patologia , Pele/patologia , Antígenos CD/análise , Antígenos de Diferenciação Mielomonocítica/análise , Biópsia , Parede Celular/química , Quimioterapia Combinada , Feminino , Células Espumosas/química , Células Espumosas/microbiologia , Células Gigantes de Corpo Estranho/química , Células Gigantes de Corpo Estranho/microbiologia , Interações Hospedeiro-Patógeno , Humanos , Hansenostáticos/uso terapêutico , Hanseníase Virchowiana/tratamento farmacológico , Lipídeos/análise , Pessoa de Meia-Idade , Mycobacterium leprae/química , Mycobacterium leprae/isolamento & purificação , Pele/microbiologia , Vacúolos
9.
J Mater Sci Mater Med ; 30(9): 103, 2019 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-31493091

RESUMO

Metal-on-metal (MoM) hip arthroplasties produce abundant implant-derived wear debris composed mainly of cobalt (Co) and chromium (Cr). Cobalt-chromium (Co-Cr) wear particles are difficult to identify histologically and need to be distinguished from other wear particle types and endogenous components (e.g., haemosiderin, fibrin) which may be present in MoM periprosthetic tissues. In this study we sought to determine whether histological stains that have an affinity for metals are useful in identifying Co-Cr wear debris in MoM periprosthetic tissues. Histological sections of periprosthetic tissue from 30 failed MoM hip arthroplasties were stained with haematoxylin-eosin (HE), Solochrome Cyanine (SC), Solochrome Azurine (SA) and Perls' Prussian Blue (PB). Sections of periprosthetic tissue from 10 cases of non-MoM arthroplasties using other implant biomaterials, including titanium, ceramic, polymethylmethacrylate (PMMA) and ultra-high molecular weight polyethylene (UHMWP) were similarly analysed. Sections of 10 cases of haemosiderin-containing knee tenosynovial giant cell tumour (TSGCT) were also stained with HE, SC, SA and PB. In MoM periprosthetic tissues, SC stained metal debris in phagocytic macrophages and in the superficial necrotic zone which exhibited little or no trichrome staining for fibrin. In non-MoM periprosthetic tissues, UHMWP, PMMA, ceramic and titanium particles were not stained by SC. Prussian Blue, but not SC or SA, stained haemosiderin deposits in MoM periprosthetic tissues and TSGT. Our findings show that SC staining (most likely Cr-associated) is useful in distinguishing Co-Cr wear particles from other metal/non-metal wear particles types in histological preparations of periprosthetic tissue and that SC reliably distinguishes haemosiderin from Co-Cr wear debris.


Assuntos
Benzenossulfonatos , Corantes/farmacologia , Análise de Falha de Equipamento/métodos , Articulação do Quadril/patologia , Nanopartículas Metálicas/análise , Próteses Articulares Metal-Metal , Coloração e Rotulagem/métodos , Artroplastia de Quadril/efeitos adversos , Artroplastia de Quadril/instrumentação , Azurina/química , Azurina/farmacologia , Benzenossulfonatos/química , Benzenossulfonatos/farmacologia , Cromo/química , Corantes/síntese química , Corantes/química , Amarelo de Eosina-(YS)/química , Amarelo de Eosina-(YS)/farmacologia , Ferrocianetos/química , Ferrocianetos/farmacologia , Células Gigantes de Corpo Estranho/efeitos dos fármacos , Células Gigantes de Corpo Estranho/patologia , Hematoxilina/química , Hematoxilina/farmacologia , Articulação do Quadril/química , Articulação do Quadril/efeitos dos fármacos , Prótese de Quadril , Técnicas Histológicas/métodos , Humanos , Macrófagos/efeitos dos fármacos , Macrófagos/patologia , Próteses Articulares Metal-Metal/efeitos adversos , Polietilenos/análise , Polietilenos/química
10.
J Cell Sci ; 133(5)2019 09 26.
Artigo em Inglês | MEDLINE | ID: mdl-31444283

RESUMO

Implanting biomaterials in tissues leads to inflammation and a foreign body response (FBR), which can result in rejection. Here, we live image the FBR triggered by surgical suture implantation in a translucent zebrafish model and compare with an acute wound response. We observe inflammation extending from the suture margins, correlating with subsequent avascular and fibrotic encapsulation zones: sutures that induce more inflammation result in increased zones of avascularity and fibrosis. Moreover, we capture macrophages as they fuse to become multinucleate foreign body giant cells (FBGCs) adjacent to the most pro-inflammatory sutures. Genetic and pharmacological dampening of the inflammatory response minimises the FBR (including FBGC generation) and normalises the status of the tissue surrounding these sutures. This model of FBR in adult zebrafish allows us to live image the process and to modulate it in ways that may lead us towards new strategies to ameliorate and circumvent FBR in humans.This article has an associated First Person interview with the first author of the paper.


Assuntos
Materiais Biocompatíveis , Reação a Corpo Estranho/patologia , Células Gigantes de Corpo Estranho/ultraestrutura , Implantes Experimentais , Animais , Adesão Celular , Forma Celular , Fibrose , Células Gigantes de Corpo Estranho/citologia , Modelos Animais , Peixe-Zebra
12.
Am J Pathol ; 189(8): 1505-1512, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-31121133

RESUMO

The presence of biomaterials and devices implanted into soft tissue is associated with development of a foreign body response (FBR), a chronic inflammatory condition that can ultimately lead to implant failure, which may cause harm to or death of the patient. Development of FBR includes activation of macrophages at the tissue-implant interface, generation of destructive foreign body giant cells (FBGCs), and generation of fibrous tissue that encapsulates the implant. However, the mechanisms underlying the FBR remain poorly understood, as neither the materials composing the implants nor their chemical properties can explain triggering of the FBR. Herein, we report that genetic ablation of transient receptor potential vanilloid 4 (TRPV4), a Ca2+-permeable mechanosensitive cation channel in the transient receptor potential vanilloid family, protects TRPV4 knockout mice from FBR-related events. The mice showed diminished collagen deposition along with reduced macrophage accumulation and FBGC formation compared with wild-type mice in a s.c. implantation model. Analysis of macrophage markers in spleen tissues and peritoneal cavity showed that the TRPV4 deficiency did not impair basal macrophage maturation. Furthermore, genetic deficiency or pharmacologic antagonism of TRPV4 blocked cytokine-induced FBGC formation, which was restored by lentivirus-mediated TRPV4 reintroduction. Taken together, these results suggest an important, previously unknown, role for TRPV4 in FBR.


Assuntos
Sinalização do Cálcio , Reação a Corpo Estranho/metabolismo , Células Gigantes de Corpo Estranho/metabolismo , Macrófagos Peritoneais/metabolismo , Mecanotransdução Celular , Canais de Cátion TRPV/metabolismo , Animais , Cálcio/metabolismo , Reação a Corpo Estranho/genética , Reação a Corpo Estranho/patologia , Células Gigantes de Corpo Estranho/patologia , Macrófagos Peritoneais/patologia , Camundongos , Camundongos Knockout , Canais de Cátion TRPV/genética
13.
BMJ Case Rep ; 12(4)2019 Apr 24.
Artigo em Inglês | MEDLINE | ID: mdl-31023740

RESUMO

Gingival pathology is a daily presentation, however a small number of systemic conditions can manifest similar to a common gingival condition and have fatal results. Dentist referred 56-year-old woman to Oral and Maxillofacial Surgery department with a 2-week medical history of gingival bleeding not responding to local measures. Biopsy showed eosinophilic infiltrate and vasculitis, and blood tests showed positive markers including cytoplasmic antineutrophil cytoplasmatic antibodies. Granulomatosis with polyangiitis is a rare disease affecting the respiratory tract, blood vessels and kidneys. Oral lesions are rarely the primary presenting feature. When left untreated, most cases are fatal within a year of diagnosis. The diagnosis can only be made when certain criteria are found, including granular oral lesions exhibiting an eosinophilic inflammatory infiltrate on biopsy. With 5% of cases showing intraoral lesions as the primary feature, it is essential that dentists have the knowledge of this rare disease to refer and not to treat as a common gingival condition.


Assuntos
Odontólogos/educação , Doenças da Gengiva/diagnóstico , Granulomatose com Poliangiite/patologia , Úlceras Orais/etiologia , Assistência ao Convalescente , Antibacterianos/uso terapêutico , Anticorpos Anticitoplasma de Neutrófilos/metabolismo , Biópsia , Diagnóstico Diferencial , Eosinófilos/patologia , Feminino , Células Gigantes de Corpo Estranho/patologia , Doenças da Gengiva/patologia , Glucocorticoides/uso terapêutico , Granulomatose com Poliangiite/sangue , Granulomatose com Poliangiite/tratamento farmacológico , Hemorragia/diagnóstico , Hemorragia/etiologia , Humanos , Pessoa de Meia-Idade , Úlceras Orais/diagnóstico , Úlceras Orais/tratamento farmacológico , Prednisolona/administração & dosagem , Prednisolona/uso terapêutico , Doenças Raras , Resultado do Tratamento , Combinação Trimetoprima e Sulfametoxazol/administração & dosagem , Combinação Trimetoprima e Sulfametoxazol/uso terapêutico
14.
J Biomed Mater Res A ; 107(4): 780-790, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-30549210

RESUMO

The biomaterials physicochemical characteristics influence their cellular reaction, degradation and regenerative capacities. Macrophages and multinucleated giant cells (MNGCs) are observed in the augmentation area of biomaterials. This study, for the first time, evaluated the polarization pattern of macrophages and MNGCs in response to two different bone substitute materials (synthetic bone substitute material [SBSM] = NanoBone vs. xenogeneic bone substitute material [XBSM] = Bio-Oss) in human bone biopsies compared to non-augmented bone (control). Histomorphometrical analysis of the polarization in proinflammatory (M1) and anti-inflammatory (M2) cells was performed using different immunohistochemical markers: CD-68 = macrophages; CCR-7 and Cox-2 (M1) and CD-206 and CD-163 (M2) and tartrate-resistant acid phosphatase (TRAP). The macrophage polarization pattern in SBSM showed a significantly higher number of M1 cells than did XBSM and non-augmented bone. XBSM induced a significantly higher number of CD-206-positive macrophages than SBSM did. No significant difference was found between XBSM and the non-augmented bone. MNGCs expressed CD-68 and TRAP. In both test-groups, MNGCs showed a high proinflammatory character (CCR-7 and Cox-2-positive) and their number in the SBSM group was significantly higher than that of XBSM. The tissue distribution showed a significantly low percentage of the remaining biomaterial in SBSM compared to XBSM. Within the limitations of this study, these findings show that MNGCs exhibit a rather proinflammatory character and lead to biomaterial degradation, once they are induced in a high number. The premature degradation of bone substitute materials is compensated with a high percentage of connective tissue and not new bone formation. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 780-790, 2019.


Assuntos
Antígenos de Diferenciação/biossíntese , Materiais Biocompatíveis/efeitos adversos , Substitutos Ósseos/efeitos adversos , Durapatita/efeitos adversos , Reação a Corpo Estranho/metabolismo , Células Gigantes de Corpo Estranho/metabolismo , Transdução de Sinais , Dióxido de Silício/efeitos adversos , Materiais Biocompatíveis/química , Substitutos Ósseos/química , Combinação de Medicamentos , Durapatita/química , Reação a Corpo Estranho/patologia , Regulação da Expressão Gênica , Células Gigantes de Corpo Estranho/patologia , Humanos , Dióxido de Silício/química
15.
J Biomed Mater Res A ; 106(10): 2726-2734, 2018 10.
Artigo em Inglês | MEDLINE | ID: mdl-30051967

RESUMO

Biomaterials can cause a chronic local inflammation called foreign body reaction, with formation of foreign body giant cells (FBGC) by monocyte/macrophage fusion. However, FBGC appearance and role for biomaterials with different physicochemical properties are not yet fully understood. This study aimed at examining FBGC and inflammatory cells after intramuscular implantation of poly(l-lactide-co-d/l-lactide) (PLA) as membranes and uncoated electro-spun fiber meshes or meshes with a positively charged plasma-polymer coating into rats. After 7, 14 and 56 days, CD68+ and CD163+ macrophages, T lymphocytes, MHC-II+ cells, FBGC, and nestin-stained tissue area as regeneration marker were morphometrically analyzed. FBGC occurrence was primarily determined by material morphology, as their numbers for meshes were 10-fold higher during acute and 50-fold higher during chronic inflammation than for membranes but comparable between uncoated and coated meshes. CD68+ macrophages decreased around and within meshes, while CD163+ macrophages and MHC-II+ cells increased within meshes. T lymphocytes within meshes were higher for coated meshes, suggesting that the peri-implant tissue immunological response is also influenced by surface chemistry. FBGC were predominantly CD68+ and CD163- , and nestin-stained tissue area was negatively correlated with CD68+ monocytes/macrophages numbers and positively correlated with CD163+ macrophages numbers, highlighting differing roles in FBGC formation and tissue regeneration. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2726-2734, 2018.


Assuntos
Células Gigantes de Corpo Estranho/patologia , Inflamação/etiologia , Poliésteres/efeitos adversos , Poliésteres/química , Próteses e Implantes/efeitos adversos , Animais , Células Apresentadoras de Antígenos/metabolismo , Antígenos CD/metabolismo , Antígenos de Histocompatibilidade Classe II/metabolismo , Inflamação/patologia , Macrófagos/metabolismo , Masculino , Ratos Endogâmicos Lew , Propriedades de Superfície , Linfócitos T/metabolismo
16.
BMJ Case Rep ; 20182018 May 12.
Artigo em Inglês | MEDLINE | ID: mdl-29754129

RESUMO

The aim of this study is to describe a case of delayed granuloma formation associated with tendon necrosis in response to Ethibond confirmed by histopathological examination and to review and discuss the related literature. A 40-year-old woman underwent a patellar tendon repair with Krakow-like #5 Ethibond sutures. Four years after the repair, she noticed progressive soreness with knee extension and swelling. An ultrasound examination revealed a proximal partial patellar tendon rupture. Based on these findings, the patient was advised to undergo surgical intervention due to a diagnosis of re-rupture. Tendinosis, fibrosis and necrosis scar tissue surrounding the previous suture were observed and excised, and samples were sent for histopathological and microbiological examination. Stripping of the patellar paratenon was performed. Surprisingly, a giant cell foreign body reaction surrounding the synthetic refringent material, as well as polymorphonuclear cells surrounding the necrotic tendon, was reported.


Assuntos
Granuloma de Células Gigantes/patologia , Traumatismos do Joelho/cirurgia , Necrose/patologia , Ligamento Patelar/patologia , Polietilenotereftalatos/efeitos adversos , Complicações Pós-Operatórias/patologia , Procedimentos Cirúrgicos Reconstrutivos/efeitos adversos , Adulto , Feminino , Reação a Corpo Estranho/patologia , Células Gigantes de Corpo Estranho/patologia , Granuloma de Células Gigantes/cirurgia , Humanos , Imageamento por Ressonância Magnética , Necrose/diagnóstico por imagem , Necrose/cirurgia , Ligamento Patelar/diagnóstico por imagem , Ligamento Patelar/cirurgia , Complicações Pós-Operatórias/imunologia , Complicações Pós-Operatórias/cirurgia , Suturas/efeitos adversos , Fatores de Tempo , Resultado do Tratamento
17.
J Oral Maxillofac Surg ; 76(8): 1719-1724, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-29608904

RESUMO

After discectomy, interpositional implants (IPIs) are believed to provide temporomandibular joint stability and protect against degenerative joint space remodeling. Alloplastic IPIs gained popularity in the late 1970s because the practice showed early success without donor site morbidity. Unfortunately, these implants were subject to substantial fragmentation resulting in an exuberant foreign body giant cell response that progressively eroded adjacent structures. Most of these alloplastic implants were removed in the years following their recall by the US Food and Drug Administration in 1991, however some remained in circulation. This report describes a case of a failed Proplast/Teflon IPI 27 years after its placement. This case highlights the considerations for managing a patient with a Proplast/Teflon IPI and serves to remind providers that, although increasingly rare, these implants are still present and subject to delayed failure.


Assuntos
Granuloma de Corpo Estranho/etiologia , Prótese Mandibular/efeitos adversos , Politetrafluoretileno/efeitos adversos , Proplast/efeitos adversos , Transtornos da Articulação Temporomandibular/cirurgia , Cefalometria , Feminino , Células Gigantes de Corpo Estranho , Granuloma de Corpo Estranho/diagnóstico por imagem , Humanos , Pessoa de Meia-Idade , Falha de Prótese , Radiografia Panorâmica , Tomografia Computadorizada por Raios X
18.
Int J Surg Pathol ; 26(6): 561-563, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-29529891

RESUMO

Pulse (hyaline ring) granuloma, a rare entity first described in lung and oral cavity, has been reported under various names before the identification of hyaline rings as fragments of pulses (the edible seeds of legumes). Similar lesions were thereafter described in extra-oral localizations, mainly the gastrointestinal tract, or localizations having potential communication with the gastrointestinal tract. Recently, 2 reports described "spiral bodies" surrounded by foreign body-type multinucleated giant cell reaction in pulse granulomas, corresponding to remnant plant vascular structures (helical xylem elements). In this article, we report a case of a 70-year-old male patient presenting to our hospital for an incisional hernia repair. He had a history of antrectomy 2 years previously for perforated duodenal ulcer complicated with fecal peritonitis. During the hernia repair procedure, multiple peritoneal whitish nodules and one subserosal appendiceal nodule were found. Appendectomy and biopsy of a peritoneal nodule were performed. Microscopic examination showed nodular lesions located in the subserosa to be pulse granulomas. Also surrounded by histiocytes, spiraled thin and rigid foreign bodies were identified. In this article, we report a case of pulse granuloma with spiral bodies complicating perforated duodenal ulcer and mimicking a peritoneal carcinomatosis. We also provide a discussion on the origin of spiral bodies in light of relevant literature.


Assuntos
Células Gigantes de Corpo Estranho/patologia , Granuloma de Corpo Estranho/patologia , Neoplasias Peritoneais/patologia , Idoso , Apendicectomia , Biópsia , Diagnóstico Diferencial , Granuloma de Corpo Estranho/diagnóstico , Granuloma de Corpo Estranho/cirurgia , Humanos , Masculino , Neoplasias Peritoneais/diagnóstico , Peritônio/patologia , Peritônio/cirurgia
19.
J Cell Physiol ; 233(1): 617-629, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-28332708

RESUMO

Monocyte fusion into osteoclasts, bone resorbing cells, plays a key role in bone remodeling and homeostasis; therefore, aberrant cell fusion may be involved in a variety of debilitating bone diseases. Research in the last decade has led to the discovery of genes that regulate osteoclast fusion, but the basic molecular and cellular regulatory mechanisms underlying the fusion process are not completely understood. Here, we reveal a role for Dyrk2 in osteoclast fusion. We demonstrate that Dyrk2 down regulation promotes osteoclast fusion, whereas its overexpression inhibits fusion. Moreover, Dyrk2 also promotes the fusion of foreign-body giant cells, indicating that Dyrk2 plays a more general role in cell fusion. In an earlier study, we showed that fusion is a cell heterotypic process initiated by fusion-founder cells that fuse to fusion-follower cells, the latter of which are unable to initiate fusion. Here, we show that Dyrk2 limits the expansion of multinucleated founder cells through the suppression of the fusion competency of follower cells.


Assuntos
Fusão Celular , Osteoclastos/enzimologia , Proteínas Tirosina Quinases/metabolismo , Animais , Reabsorção Óssea , Diferenciação Celular , Proliferação de Células , Regulação Enzimológica da Expressão Gênica , Células Gigantes de Corpo Estranho/enzimologia , Camundongos , Camundongos Endogâmicos C57BL , Fatores de Transcrição NFATC/genética , Fatores de Transcrição NFATC/metabolismo , Proteínas Tirosina Quinases/genética , Células RAW 264.7 , Interferência de RNA , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Transdução de Sinais , Fatores de Tempo , Transfecção
20.
J Biomed Mater Res A ; 106(3): 746-757, 2018 03.
Artigo em Inglês | MEDLINE | ID: mdl-29052368

RESUMO

The foreign body reaction (FBR) against an implanted device is characterized by the formation of a fibrotic tissue around the implant. In the case of interfaces for peripheral nerves, used to stimulate specific group of axons and to record different nerve signals, the FBR induces a matrix deposition around the implant creating a physical separation between nerve fibers and the interface that may reduce its functionality over time. In order to understand how the FBR to intraneural interfaces evolves, polyimide non-functional devices were implanted in rat peripheral nerve. Functional tests (electrophysiological, pain and locomotion) and histological evaluation demonstrated that implanted devices did not cause any alteration in nerve function, in myelinated axons or in nerve architecture. The inflammatory response due to the surgical implantation decreased after 2 weeks. In contrast, inflammation was higher and more prolonged in the device implanted nerves with a peak after 2 weeks. With regard to tissue deposition, a tissue capsule appeared soon around the devices, acquiring maximal thickness at 2 weeks and being remodeled subsequently. Immunohistochemical analysis revealed two different cell types implicated in the FBR in the nerve: macrophages as the first cells in contact with the interface and fibroblasts that appear later at the edge of the capsule. Our results describe how the FBR against a polyimide implant in the peripheral nerve occurs and which are the main cellular players. Increasing knowledge of these responses will help to improve strategies to decrease the FBR against intraneural implants and to extend their usability. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 746-757, 2018.


Assuntos
Materiais Biocompatíveis/efeitos adversos , Reação a Corpo Estranho/patologia , Imidas/efeitos adversos , Implantes Experimentais , Fibras Nervosas/patologia , Animais , Proteínas de Ligação ao Cálcio/metabolismo , Feminino , Células Gigantes de Corpo Estranho , Inflamação/patologia , Macrófagos/metabolismo , Proteínas dos Microfilamentos/metabolismo , Ratos Sprague-Dawley , Fatores de Tempo
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