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1.
Spectrochim Acta A Mol Biomol Spectrosc ; 264: 120229, 2022 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-34371316

RESUMO

In this paper, a one-dimensional shallow convolutional neural network structure combined with elastic nets (1D-SCNN-EN) was firstly proposed to predict the glucose concentration of blood by Raman spectroscopy. A total of 106 different blood glucose spectra were obtained by Fourier transform (FT) Raman spectroscopy. The one-dimensional shallow convolutional neural network, with elastic nets added to the full connected layer, was presented to capture multiple deep features and reduce the complexity of the model. The 1D-SCNN-EN model has a better performance than conventional approaches (partial least squares and support vector machine). The root mean squared error of calibration (RMSEC), the root mean squared error of prediction (RMSEP), the determination coefficient of prediction (RP2), and the residual predictive deviation of prediction (RPD) were 0.10262, 0.11210, 0.99403, and 12.94601, respectively. The experiment results showed that the 1D-SCNN-EN model has a higher prediction accuracy and stronger robustness than the other regression models. The overall studies indicated that the 1D-SCNN-EN model looked promising for predict the glucose concentration of blood by Raman spectroscopy when the sample size is small.


Assuntos
Glicemia , Análise Espectral Raman , Glucose , Análise dos Mínimos Quadrados , Redes Neurais de Computação
2.
Food Chem ; 366: 130530, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34303204

RESUMO

In this study, the effects of different temperatures, incubation times and types of reducing sugars, including glucose and different low molecular weight (Mw) chito-oligosaccharides (COS) with varying acetylation degree (AD), on the extent of Maillard reaction (MR) on chitosan-based films were studied. Interestingly, an improvement of structural and functional properties of all MR-crosslinked films was noted, which is more pronounced by heating at higher temperature and exposure time. These findings were proved through Fourier-transform infrared and X-ray diffraction analyses. In addition, color change and Ultraviolet spectra demonstrate that glucose addition provides the high extent of MR, followed by COS1 (Mw < 4.4 kDa; AD, 18.20%) and COS2 (Mw < 4.4 kDa; AD, 10.63%). These results were confirmed by enhanced water resistance and thermal properties. Moreover, MR-chitosan/COS films showed the highest mechanical properties, whereas, glucose-loaded films were brittle, as demonstrated by scanning electron microscopy micrographs. Furthermore, MR-chitosan/COS1 films exhibited the better antioxidant behavior followed by chitosan/glucose and chitosan/COS2 films, mainly at higher heating-conditions. Thereby, MR-crosslinked chitosan/COS based films were attractive to be applied as functional and active coating-materials in various fields.


Assuntos
Quitosana , Antioxidantes , Glucose , Reação de Maillard , Peso Molecular , Espectroscopia de Infravermelho com Transformada de Fourier
3.
Food Chem ; 370: 130901, 2022 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-34500289

RESUMO

The development of Aspergillus niger (A. niger) spores as glucose oxidase (GOD) biocatalysts to produce gluconic acid is highly anticipated in the food industry. Herein, a piezoelectric sensor (PIS) method has been developed for the detection of GOD activity and better application of rapid screening of GOD activity in A. niger spores. The GOD activity detection is based on GOD catalyzing ß-d-glucose to produce gluconic acid, which results in frequency shift changes recorded by the PIS device in real-time. Using the PIS method, the kinetic parameter 6.5 mg/mL, the correlation equation υ0=31.92CGOD+1.04, the recoveries (89.4%-93.9%, and their RSDs were all within 6.1%) and the optimal GOD activity in A. niger spores under different treatment conditions was obtained. Compared with the classical methods, the proposed method is accurate, rapid, convenient and does not require additional reagents. It has a broad range of potential applications for exploring new GOD biocatalysts.


Assuntos
Aspergillus niger , Glucose Oxidase , Glucose , Esporos Fúngicos
4.
Spectrochim Acta A Mol Biomol Spectrosc ; 266: 120432, 2022 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-34607092

RESUMO

Diabetes has become one of the three chronic non-communicable diseases threatening human health in the world, and the detection of glucose concentration is of great importance for the prevention and treatment of diabetes. The noninvasive detection of glucose in tears has attracted interest over the past several decades, however, time-consuming, expensive equipment, and specialist technicians make tear analysis still challenging. Here, flexible surface-enhanced Raman scattering (SERS) substrates composed of gold nanoparticles (AuNPs) and two-dimensional MXene Ti3C2TX nanosheets have been designed. The GMXeP (gold nanoparticles with MXene nanosheets loaded on paper) SERS substrates show good sensitivity, reproducibility, and stability, yielding an enhancement factor (EF) of 3.7 × 105 at the concentration of 10-9 M. The GMXeP SERS substrates are used to detect glucose of diabetic tears within a linear range of 1-50 µM, the lowest detection concentration is 0.39 µM and the significant correlation between tear glucose and blood glucose indicates that this method is suitable for sensitive and noninvasive detection of blood glucose.


Assuntos
Diabetes Mellitus , Nanopartículas Metálicas , Glucose , Ouro , Humanos , Reprodutibilidade dos Testes , Análise Espectral Raman
5.
Spectrochim Acta A Mol Biomol Spectrosc ; 264: 120299, 2022 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-34474221

RESUMO

The optical sensor with ratiometric and turn-off dual modes is constructed to detect H2O2 and glucose based on blue fluorescent carbon dots (CDs) and MnO2 nanosheets with great ability of fluorescence quenching and scattering. Employing CDs@MnO2 nanosheets nanocomposite as the probe, H2O2 is detected by simultaneously collecting first-order scattering (FOS, 353.5 nm), fluorescence (440 nm), and second-order scattering (SOS, 710 nm) under the excitation of 350 nm. H2O2 with strong oxidation property can etch the lamellar structure of MnO2 nanosheets into nano-fragments, which made the fluorescence of CDs in the system recover and the scattering intensity (FOS and SOS) of the system decrease significantly. Therefore, the optical sensor combined FOS and fluorescence signals in ratiometric mode, and SOS signal in turn-off mode to realize sensitive determination of H2O2. The linear ranges of ratiometric mode and turn-off mode for H2O2 detection were 0.2-40 and 0.2-15 µM, respectively. And the limits of detection (LODs) of two modes were 73 and 104 nM, respectively. Furthermore, the sensor was also successfully applied to the detection of glucose which can react to produce H2O2. Satisfactorily, the LODs of this sensor for glucose detection were 95 and 113 nM for ratiometric mode and turn-off mode, respectively. This work not only provides a new method for the accurate detection of H2O2 and glucose, but also extends a new idea for the study of the combination of scattering and fluorescence.


Assuntos
Carbono , Pontos Quânticos , Corantes Fluorescentes , Glucose , Peróxido de Hidrogênio , Compostos de Manganês , Óxidos
6.
Food Chem ; 369: 130940, 2022 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-34474292

RESUMO

Berries are rich food sources of potentially health-beneficial (poly)phenols. However, they may undergo chemical modifications during gastrointestinal digestion. The effect of simulated gastrointestinal digestion on the content and composition of secondary metabolites from Gaultheria phillyreifolia and G. poeppigii berries was studied. The influence of the digested extracts on the in vitro metabolism and absorption of carbohydrates was evaluated. After simulated digestion, 31 compounds were detected by UHPLC-DAD-MS. The total content of anthocyanins decreased by 98-100%, flavonols by 44-56%, phenylpropanoids by 49-75% and iridoids by 33-45%, the latter showing the highest stability during digestion. Digested extracts inhibited α-glucosidase (IC50 2.8-24.9 µg/mL) and decreased the glucose uptake in Caco-2 cells by 17-28%. Moreover, a decrease in the mRNA expression of glucose transporters SGLT1 (38-92%), GLUT2 (45-96%), GLUT5 (28-89%) and the enzyme sucrase-isomaltase (82-97%) was observed. These results show the effect of simulated gastrointestinal digestion on the content and composition of Gaultheria berries.


Assuntos
Gaultheria , Polifenóis , Antocianinas , Antioxidantes , Células CACO-2 , Digestão , Frutas/química , Glucose , Humanos , Iridoides , Extratos Vegetais , Polifenóis/análise
7.
Biosens Bioelectron ; 195: 113586, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34455144

RESUMO

Enzyme-based biosensors are sensitive to temperature due to their strong temperature dependency of catalytic activity. Aiming at enhancing biosensing detection for glucose assay over a wide range of applicable temperatures, we designed a thermal self-regulatory intelligent biosensor through an innovative integration of phase change material (PCM) and bioelectrocatalytic substances. An electroactive phase-change microcapsule system was firstly fabricated by microencapsulating n-docosane as a PCM core in the SiO2 shell, followed by depositing polydopamine along with carbon nanotubes as an electroactive layer on the surface of SiO2 shell. The resultant microcapsules showed a regularly spherical morphology and well-defined core-shell microstructure. They also exhibited a satisfactory latent heat capacity of around 137 J/g for implementing temperature regulation with a good working stability. An electrochemical biosensing system was constructed with the resultant electroactive microcapsules together with glucose oxidase as a redox enzyme, achieving a thermal self-regulation capability to enhance the biosensing detection of glucose under in-situ thermal management at higher temperatures. With a high sensitivity of 5.95 µA⋅mM-1⋅cm-2 and a lower detection limit of 13.11 µM at 60 °C, the intelligent biosensor developed by this study demonstrated a superior determination capability and better detection performance toward glucose than conventional biosensors in a high temperature region thanks to effective regulation of microenvironment temperature in the electrode system. This study provides a promising strategy for the development of thermal self-regulatory smart biosensors with an enhanced identification ability to detect various chemical substances over a wide range of applicable temperatures.


Assuntos
Técnicas Biossensoriais , Nanotubos de Carbono , Cápsulas , Eletrodos , Enzimas Imobilizadas , Glucose , Glucose Oxidase , Dióxido de Silício
8.
Chemosphere ; 286(Pt 1): 131642, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34351280

RESUMO

Neonicotinoids are replacement insecticides increasingly used for organophosphates, methylcarbamates, and pyrethroids. Experimental evidence suggests neonicotinoids may affect glucose metabolism and insulin secretion through pancreatic ß cell dysfunction, oxidative stress, and inflammation. However, no epidemiologic study has investigated neonicotinoids as potential diabetogens. We examined associations between neonicotinoids with insulin and glucose homeostasis parameters among 1381 non-diabetic adults in the National Health and Nutrition Examination Survey (2015-2016). Urinary concentrations of acetamiprid, clothianidin, imidacloprid, N-desmethyl-acetamiprid, and 5-hydroxy-imidacloprid were quantified. Fasting plasma glucose, insulin, and hemoglobin A1c (HbA1c) were assessed. Insulin resistance was defined as a homeostatic model assessment of insulin resistance ≥2.5. We used weighted linear and logistic regression to estimate associations between detectable neonicotinoids with insulin and glucose homeostasis parameters compared to non-detectable neonicotinoid concentrations. Weighted detection frequencies for imidacloprid, 5-hydroxy-imidacloprid, and N-desmethyl-acetamiprid were 4.4 %, 21.5 %, and 32.8 %, respectively. Detectable imidacloprid (ß = -4.7 µIU/mL, 95 % confidence interval [CI] -8.5, -0.8) and 5-hydroxy-imidacloprid (ß = -2.4 µIU/mL, 95 % CI -4.6, -0.2) were associated with lower fasting plasma insulin levels. Individuals with detectable 5-hydroxy-imidacloprid had lower odds of insulin resistance (odds ratio [OR] = 0.3, 95 % CI 0.2, 0.7). We observed evidence of sexually dimorphic associations between N-desmethyl-acetamiprid with glucose (pint = 0.079) and 5-hydroxy-imidacloprid with HbA1c (pint = 0.038), with patterns suggesting positive associations in males and negative associations in females. Associations between 5-hydroxy-imidacloprid and insulin were modified by body mass index (BMI) (pint = 0.013). We additionally observed age modified associations between 5-hydyroxy-imidacloprid and glucose (pint = 0.048). Results suggest neonicotinoids may be associated with insulin and glucose homeostasis indices and call for prospective studies to examine the metabolic impact of these replacement insecticides in humans.


Assuntos
Inseticidas , Insulina , Adulto , Feminino , Glucose , Homeostase , Humanos , Inseticidas/toxicidade , Masculino , Neonicotinoides , Nitrocompostos , Inquéritos Nutricionais , Estudos Prospectivos
9.
Food Chem ; 370: 131015, 2022 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-34509943

RESUMO

The present study investigates the substitute of apple juice concentrate with some cheap sweeteners including glucose syrup, fructose syrup, and date concentrate, as the most common adulterants. For this purpose, pure and authenticated apple juice concentrate was individually adulterated with 10% to 50% of glucose syrup, fructose syrup, and date concentrate. High-performance liquid chromatography coupled with a refractive index detector (HPLC-RID) was applied to determine the carbohydrates profile of samples. The results of HPLC-RID were subjected to multivariate statistical analysis, namely principal component analysis (PCA) and linear discriminant analysis (LDA). The results showed that the glucose/fructose ratio and maltose content were the best indicators to detect adulteration of apple juice concentrate. A set of glucose, sorbitol, sucrose, maltose, and glucose/fructose ratio was used as a discriminating factor. Using this approach, adulteration of apple juice concentrate with cheaper sweeteners was detected at a limit of 10%, depending on the adulterant.


Assuntos
Malus , Cromatografia Líquida de Alta Pressão , Contaminação de Alimentos/análise , Frutose , Glucose , Refratometria
10.
Spectrochim Acta A Mol Biomol Spectrosc ; 265: 120401, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34555694

RESUMO

Chemiluminescent (CL) reaction between hydrogen peroxide (H2O2) and luminol was dramatically enhanced by sodium molybdate (Na2MoO4) for 284-fold. CL mechanism investigation indicated that Na2MoO4 increased the production of hydroxyl radical (•OH) and superoxide anion (•O2-) in the H2O2-luminol system, which could attribute to the enhanced-CL intensity and gave us new insights into the CL-enhanced property of Na2MoO4. The CL intensity of Na2MoO4-H2O2-luminol system increased with the concentration of H2O2, based on which, a convenient and sensitive CL determination method could be developed for H2O2 in the concentration ranging from 0.5 to 60 µmol/L, with a detection limit of 0.25 µmol/L. Combining with glucose oxidase, the Na2MoO4-H2O2-luminol system could also be applied for glucose detection. Glucose in human serum has been successfully detected with satisfied recoveries in the range of 96.7 % to 105.4 %.


Assuntos
Técnicas Biossensoriais , Luminescência , Glucose , Humanos , Peróxido de Hidrogênio , Limite de Detecção , Medições Luminescentes , Luminol , Molibdênio
11.
Spectrochim Acta A Mol Biomol Spectrosc ; 265: 120376, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34571376

RESUMO

Metal-organic framework (MOF) MOF-Fe nanosols were prepared, which exhibits strongly catalysis of the new fluorescence indicator reaction of 3, 3', 5, 5'-tetramethylbenzidine (TMB)-H2O2 to produce the oxidation product TMBOX. The TMBOX fluorescent probe has a strong fluorescence peak at 405 nm. After optimizing the various conditions for the determination of H2O2 system and glucose system, the linear range of fluorescence determination of H2O2 was 0.75-7.5 µM, and the detection limit was 0.3 µM. Since H2O2 is the product of glucose oxidase (GOD) catalyzed oxidation of glucose, and a simple and convenient fluorescence method was also established for glucose. The results show that the glucose concentration in the range of 0.2-20 µM has a good correlation with the fluorescence intensity, and the detection limit of glucose was 0.1 µM. This method has been used to detect the content of glucose in drinks with satisfactory results.


Assuntos
Peróxido de Hidrogênio , Estruturas Metalorgânicas , Catálise , Colorimetria , Glucose , Glucose Oxidase/metabolismo , Limite de Detecção , Oxirredução
12.
J Sci Food Agric ; 102(1): 53-61, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34031881

RESUMO

BACKGROUND: Corn silk is a very important by-product of corn production with medicinal value. Corn silk polysaccharide (CSP) is the main active ingredient. In the present study, ultrasound and spheroidization by anti-solvent were applied to improve the biological activity of CSP. RESULTS: The results showed that ultrasonic degradation improved the α-glucosidase inhibitory activity of CSP by changing its physicochemical characteristics. As the anti-solvent ratio increased, the particle size of the nanoparticles (NPs) from the spheroidization of ultrasonic-degraded corn silk polysaccharide (UCSP) gradually increased, and NP-1 exhibited the highest inhibitory effect of α-glucosidase. Isothermal titration calorimetry (ITC) results indicated that the enhanced activity might be due to more α-glucosidase binding sites with NP-1 compared with no spheroidization. Western blotting results showed that NP-1 could improve the 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-d-glucose (2-NBDG) uptake in the L6 cells by regulating the phosphatidylinositol 3-kinase (PI3K)/Akt signal pathway and the translocation of glucose transporter 4 (GLUT4). NP-1 also exhibited excellent stability in different environments. CONCLUSION: The study revealed that ultrasonic treatment and spheroidization processing showed potential applications for improving the biological activity of polysaccharides. © 2021 Society of Chemical Industry.


Assuntos
Extratos Vegetais/química , Extratos Vegetais/farmacologia , Polissacarídeos/química , Polissacarídeos/farmacologia , Zea mays/química , Animais , Transporte Biológico/efeitos dos fármacos , Linhagem Celular , Glucose/metabolismo , Transportador de Glucose Tipo 4/genética , Transportador de Glucose Tipo 4/metabolismo , Inibidores de Glicosídeo Hidrolases/química , Fosfatidilinositol 3-Quinase/genética , Fosfatidilinositol 3-Quinase/metabolismo , Proteínas Proto-Oncogênicas c-akt/genética , Proteínas Proto-Oncogênicas c-akt/metabolismo , Ratos , Transdução de Sinais/efeitos dos fármacos , Ultrassom , alfa-Glucosidases/química
13.
Talanta ; 237: 122954, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34736679

RESUMO

Herein, a simple microfluidic paper-based analytical device (µPAD) by using platinum nanoparticles (Pt NPs) as highly active peroxidase mimic for simultaneous determination of glucose and uric acid was fabricated. The µPAD consisted of one sample transportation layer, four paper-based detection chips, and two layers of hydrophobic polyethylene terephthalate (PET) films. The four detection chips were immobilized with various chromogenic reagents, Pt NPs, and specific oxidase (glucose oxidase or uricase). H2O2 generated by specific enzymatic reactions could oxidize co-immobilized chromogenic reagents to produce colored products by using Pt NPs as efficient catalyst. The multi-layered structure of µPAD could effectively improve the color uniformity and color intensity. Total color intensity from each two detection chips modified with distinct chromogenic reagents were used for quantitative analysis of glucose and uric acid, respectively, resulting in significantly improved sensitivity. The linear range for glucose and uric acid detection was 0.01-5.0 mM and 0.01-2.5 mM, respectively. Satisfied results were obtained for glucose and uric acid detection in real serum samples. An easy-to-use smartphone APP was developed for convenient and intelligent detection. The developed µPAD integrated with smartphone as detector holds great applicability for simple and portable on-site analysis.


Assuntos
Nanopartículas Metálicas , Técnicas Analíticas Microfluídicas , Colorimetria , Glucose , Peróxido de Hidrogênio , Microfluídica , Papel , Peroxidase , Peroxidases , Platina , Smartphone , Ácido Úrico
14.
BMJ Case Rep ; 14(11)2021 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-34728514

RESUMO

Fanconi-Bickel syndrome (FBS) is a rare autosomal recessive disease, resulting from mutations in the SLC2A2 gene, causing impaired glucose transporter 2 protein transporter protein function, impaired glucose and galactose utilisation, hepatorenal glycogen accumulation and organ dysfunction. Clinical features include failure to thrive, hepatomegaly, rickets, short stature and delayed puberty. Therapy includes electrolyte supplementation and uncooked cornstarch. We present a 15-year-old boy diagnosed with FBS in infancy. Growth velocity was normal on standard treatment until age 8.5 years, at which time growth failure led to a diagnosis of acquired growth hormone (GH) deficiency. Initiation of recombinant human GH (rhGH) replacement of 0.25 µg/kg/week resulted in marked improvement in growth velocity and height. While short stature is expected in FBS, growth velocity that falls below the normal range despite adequate therapy should prompt further evaluation. Our case suggests that acquired GH deficiency can arise in FBS and benefits from rhGH therapy.


Assuntos
Síndrome de Fanconi , Adolescente , Criança , Insuficiência de Crescimento , Síndrome de Fanconi/diagnóstico , Síndrome de Fanconi/tratamento farmacológico , Síndrome de Fanconi/genética , Glucose , Hormônio do Crescimento , Humanos , Masculino , Mutação
15.
Zhongguo Dang Dai Er Ke Za Zhi ; 23(11): 1174-1183, 2021 Nov 15.
Artigo em Inglês, Chinês | MEDLINE | ID: mdl-34753551

RESUMO

OBJECTIVES: To study the effect of high-fat diet for maternal Sprague-Dawley rats at different stages on glucose and lipid metabolism in offspring and related mechanisms. METHODS: According to the diet before pregnancy and during pregnancy and lactation, maternal rats were randomly divided into four groups (n=9 each): CC (control diet before pregnancy and during pregnancy and lactation), HC (high-fat diet before pregnancy and control diet during pregnancy and lactation), CH (control diet before pregnancy and high-fat diet during pregnancy and lactation), and HH (high-fat diet before pregnancy and during pregnancy and lactation), and all offspring rats were given control diet after weaning. The body weight of maternal rats was recorded before and during pregnancy. Male offspring rats were selected from each group at the juvenile stage (3-week old) and the adult stage (12-week old) to measure the levels of fasting blood glucose (FBG) and fasting insulin (FINS) and the levels of triglyceride (TG) and total cholesterol (TC) in the liver. Homeostasis Model Assessment of Insulin Resistance (HOMA-IR) index was calculated, and the area under the curve (AUC) was calculated for glucose tolerance test (GTT) and insulin tolerance test (ITT). Lipid deposition in the liver was observed, and the mRNA and protein expression levels of the key genes in glucose and lipid metabolism (IR, IRS, and AKT), FASN, SREBP1c, and PPARα in the liver were also measured. RESULTS: Compared with the control diet groups (CC and CH groups), the groups with high-fat diet before pregnancy (HC and HH groups) had a significant increase in body weight (P<0.001). Compared with the CC group, the HC, CH, and HH groups had a significantly greater increase in body weight (P<0.001). Compared with the CC group, the HC, CH, and HH groups had significant increases in body weight, the levels of TG and TC in the liver, and the mRNA and protein expression levels of FASN, SREBP1c, and PPARα in the offspring rats at week 3 after birth (P<0.05), as well as a significant increase in lipid deposition in the liver, with the most significant increase of the parameters in the HH group. Compared with the CC group, the HH group had significant increases in the levels of FBG and FINS, HOMA-IR index, GTT-AUC, ITT-AUC, and the protein expression level of p-IRS in the liver and significant reductions in the mRNA and protein expression levels of IR and IRS in the liver in the offspring rats at week 3 after birth (P<0.05). Compared with the CC group, the HC, CH, and HH groups had significant increases in body weight, the levels of FBG and FINS, HOMA-IR index, GTT-AUC, ITT-AUC, the levels of TG and TC in the liver, protein expression level of p-IRS in the liver, and the mRNA and protein expression levels of FASN, SREBP1c, and PPARα in the offspring rats at week 12 after birth (P<0.05), as well as a significant increase in lipid deposition in the liver, with the most increase of the parameters in the HH group. Compared with the CC group, the HC, CH, and HH groups had significant reductions in the mRNA expression levels of IR, IRS, and AKT and the protein expression levels of IR, IRS, and p-AKT in the offspring rats at week 12 after birth (P<0.05). There were no significant differences in the levels of glucose and lipid metabolism between the HC and CH groups at various stages (P>0.05). CONCLUSIONS: High-fat diet for rats at different stages before and after pregnancy has different effects on glucose and lipid metabolism of offspring rats, and high-fat diet before pregnancy and during pregnancy and lactation has the greatest effect. The effect of high-fat diet on glucose and lipid metabolism of offspring rats is considered associated with the changes in the expression of genes involved in glucose and lipid metabolism.


Assuntos
Dieta Hiperlipídica , Glucose , Resistência à Insulina , Metabolismo dos Lipídeos , Animais , Peso Corporal , Dieta Hiperlipídica/efeitos adversos , Feminino , Glucose/metabolismo , Insulina , Fígado/metabolismo , Masculino , Gravidez , Ratos , Ratos Sprague-Dawley
16.
Ann Palliat Med ; 10(10): 10887-10895, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-34763450

RESUMO

BACKGROUND: Heart failure (HF) often complicates atrial fibrillation (AF) and conversely, AF frequently complicates HF. Furthermore, the coexistence of both conditions significantly increases the risk of cardiovascular complications. Sodium-glucose cotransporter-2 (SGLT-2) inhibitors have been demonstrated to decrease hospitalizations for HF and reduce cardiovascular death. Therefore, this study evaluated the effects of SGLT-2 inhibitors on AF in patients with HF. METHODS: A total of 903 patients with HF were enrolled in this study. Basic patient data including demographic characteristics, medical history, cardiovascular medications, and results of biochemical tests were collated. Logistic regression analysis was performed to examine the association between SGLT-2 inhibitors and the risk of AF. The effects of SGLT-2 inhibitors on AF were further analyzed according to subgroups. RESULTS: Patients treated with SGLT-2 inhibitors experienced a lower prevalence of AF (8.4% vs. 12.1%, P<0.001) compared to patients without SGLT-2 treatment. Controlling for potential confounders revealed that SGLT-2 inhibitors decreased the risk of AF by 24% [odds ratio (OR): 0.76; 95% confidence interval (CI): 0.70-0.85; P<0.001]. The effect of SGLT-2 inhibitors on AF was consistent in patients aged < 65 years and patients aged ≥65 years (OR =0.82 and 95% CI: 0.71-0.88 vs. OR =0.84 and 95% CI: 0.77-0.92, respectively; P interaction =0.501). Similarly, neither gender, body mass index (BMI), estimated glomerular filtration rate (eGFR), nor the New York Heart Association (NYHA) classification affected the protective effect of SGLT-2 inhibitors. CONCLUSIONS: SGLT-2 inhibitors reduced the risk of AF in patients with HF, and the effect was consistent irrespective of age, gender, BMI, eGFR, and NYHA classification.


Assuntos
Fibrilação Atrial , Diabetes Mellitus Tipo 2 , Insuficiência Cardíaca , Inibidores do Transportador 2 de Sódio-Glicose , Fibrilação Atrial/tratamento farmacológico , Diabetes Mellitus Tipo 2/complicações , Diabetes Mellitus Tipo 2/tratamento farmacológico , Glucose , Insuficiência Cardíaca/tratamento farmacológico , Insuficiência Cardíaca/prevenção & controle , Humanos , Fatores de Risco , Sódio , Inibidores do Transportador 2 de Sódio-Glicose/uso terapêutico
17.
Anal Chim Acta ; 1188: 339188, 2021 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-34794574

RESUMO

A CoFe Prussian blue analogue (CoFe PB) modified FTO electrode, prepared via a facile electrodeposition method, is investigated as a non-enzymatic glucose sensor under neutral conditions. The electrode exhibits a linear detection of glucose in the 0.1-8.2 mmol/L range with a detection limit of 67 µM, a sensitivity of 18.69 µA/mM.cm2, and a fast response time of less than 7 s under neutral conditions. Its stability is confirmed with both electrochemical experiments and characterization studies performed on the pristine and post-mortem electrode. We also conducted a comprehensive electrochemical analysis to elucidate the identity of the active site and the glucose oxidation mechanism on the Prussian blue surface.


Assuntos
Técnicas Eletroquímicas , Glucose , Cobalto , Eletrodos , Ferrocianetos
18.
Rev Esp Salud Publica ; 952021 Nov 12.
Artigo em Espanhol | MEDLINE | ID: mdl-34728595

RESUMO

OBJECTIVE: The therapeutic arsenal of hypoglycaemic drugs has been increased by the commercialization of new molecules in recent years. The main objective of this study was to know the trend of use of this group of drugs in a healthcare area. METHODS: The data was obtained through an electronic medical prescription billing computer application that contains all the information on the consumption of prescription drugs. The last seven years were analyzed in a healthcare area that covers a population of 85 thousand inhabitants and the defined daily dose (DDD) per inhabitant per day was used as a measurement variable. RESULTS: In our health area, an increasing and constant use of antidiabetic drugs was observed from a value of 55.62 DHD per inhabitant per day in 2014 to a value of 65.04 in 2020. Throughout the period studied, oral hypoglycemic drug combinations and sodium-glucose cotransporter-2 inhibitors were the therapeutic subgroups that experienced growth greater than 50%. CONCLUSIONS: The consumption of hypoglycemic agents experienced a notable growth in the period analyzed, mainly at the expense of new therapeutic groups and new commercialized molecules.


Assuntos
Diabetes Mellitus Tipo 2 , Preparações Farmacêuticas , Inibidores do Transportador 2 de Sódio-Glicose , Atenção à Saúde , Diabetes Mellitus Tipo 2/tratamento farmacológico , Glucose , Humanos , Hipoglicemiantes , Espanha
19.
Nan Fang Yi Ke Da Xue Xue Bao ; 41(10): 1527-1533, 2021 Oct 20.
Artigo em Chinês | MEDLINE | ID: mdl-34755668

RESUMO

OBJECTIVE: To investigate the effect of dissipating phlegm and blood stasis simultaneously for protecting cardiac microvascular endothelial cells (CMECs) against high glucose-induced injury and the role of AGEs/RAGE axis in the underlying mechanism. METHODS: The primary CMECs were isolated from rat heart by enzymatic digestion and identified by immunofluorescence assay. The CMECs exposed to 33 mmol/L glucose for 48 h were divided into model group (MC), resolving phlegm (RP) group, dissipating blood stasis (DBS) group, dissipating phlegm and blood stasis (RPDBS) group and ALT-711 group. After treatment with 10% drug-containing serum and ALT-711 for 48 h, the content of AGEs in the cells were measured with ELISA. The expressions of RAGE mRNA and protein were measured with real-time quantitative PCR, immunofluorescence assay and Western blotting; The activity of NADPH oxidase and ROS level were measured by cytochrome c reduction and fluorescent probe DHE. RESULTS: High glucose exposure significantly increased the content of AGEs, RAGE expressions at the protein and mRNA levels, NADPH oxidase activity and ROS level in the CMECs (P < 0.01). These changes were significantly mitigated by treatments with RP, DBS, RPDBS and ALT-711 (P < 0.01), among which RPDBS caused the most significant decrements in AGEs content, RAGE expression and NADPH oxidase activity (P < 0.01, P < 0.05). The reduction of ROS level in the RPDBS group was significantly greater than that in RP group (P < 0.01), but similar to that in DBS group (P > 0.05). CONCLUSION: Dissipating phlegm and blood stasis simultaneously can be helpful for prevention and treatment of diabetic myocardial microangiopathy by suppressing the excessive activation of AGEs-RAGE signal axis and oxidative stress, thus protecting CMECs against high glucose-induced damage. Dissipating phlegm and blood stasis simultaneously is better than either of the therapy alone.


Assuntos
Diabetes Mellitus , Células Endoteliais , Animais , Glucose , Miocárdio , Estresse Oxidativo , Ratos
20.
Sensors (Basel) ; 21(21)2021 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-34770294

RESUMO

Blood glucose (BG) concentration monitoring is essential for controlling complications arising from diabetes, as well as digital management of the disease. At present, finger-prick glucometers are widely used to measure BG concentrations. In consideration of the challenges of invasive BG concentration measurements involving pain, risk of infection, expense, and inconvenience, we propose a noninvasive BG concentration detection method based on the conservation of energy metabolism. In this study, a multisensor integrated detection probe was designed and manufactured by 3D-printing technology to be worn on the wrist. Two machine-learning algorithms were also applied to establish the regression model for predicting BG concentrations. The results showed that the back-propagation neural network model produced better performance than the multivariate polynomial regression model, with a mean absolute relative difference and correlation coefficient of 5.453% and 0.936, respectively. Here, about 98.413% of the predicted values were within zone A of the Clarke error grid. The above results proved the potential of our method and device for noninvasive glucose concentration detection from the human wrist.


Assuntos
Glicemia , Glucose , Automonitorização da Glicemia , Metabolismo Energético , Humanos , Aprendizado de Máquina
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