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1.
Food Chem ; 368: 130768, 2022 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-34392120

RESUMO

Apoptosis plays a critical role in sea cucumber autolysis. To investigate the ultraviolet (UV)-induced apoptosis, sea cucumbers with and without injection of BAPTA-AM (cytosolic calcium chelator) were exposed to UV (15 W/m2) for 30 min. The results showed that UV irradiation caused several changes in sea cucumber coelomocytes, including calcium imbalance, abnormal morphology of endoplasmic reticulum, upregulation of pro-apoptotic proteins CRT, CHOP, and caspases 9 and 3, and downregulation of anti-apoptotic protein Bcl-2. A comparison between the two groups showed that injection of the calcium chelator into sea cucumbers helped maintain coelomocyte intracellular calcium homeostasis and suppressed other abnormal changes caused by ER stress, indicating apoptosis in sea cucumbers is mediated by calcium imbalance and follows the activation of the ER stress pathway. Therefore, this study broadens understanding of the apoptotic mechanism involved in sea cucumber autolysis, which is helpful in developing preservative agents for sea cucumbers.


Assuntos
Pepinos-do-Mar , Stichopus , Animais , Autólise , Cálcio , Homeostase
2.
Chemosphere ; 286(Pt 1): 131625, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34303901

RESUMO

Captan is a non-systematic fungicide widely used in agricultural production, and its residues have been found in the environment and daily diet. Previous studies confirmed that captan exerts several toxic effects on tissues, but its effect on the mammalian female reproductive system is unclear. In current study, we reported that captan affected mouse ovarian homeostasis and disrupted female hormone receptor expression, leading to impaired follicular development. Ovarian follicles from the captan exposure group showed an increased level of inflammation, endoplasmic reticulum stress and apoptosis. In addition, captan exposure disrupted oocyte development. Transcriptomic analysis indicated that captan changed multiple genes expression in oocytes, including autophagy and apoptosis. Further molecular testing showed that captan induced oxidative stress and mitochondrial dysfunction, as indicated by the increased level of reactive oxygen species, disrupted mitochondrial structure and distribution, and depolarized membrane potential. Furthermore, captan triggered DNA damage, autophagy and early apoptosis, as shown by the enhanced levels of γ-H2AX, LC3, and Annexin-V and increased expression of related genes. Taken together, these results indicated that captan exposure impairs ovarian homeostasis and subsequently affects oocyte development.


Assuntos
Captana , Oócitos , Animais , Apoptose , Captana/metabolismo , Feminino , Homeostase , Camundongos , Mitocôndrias/metabolismo , Oócitos/metabolismo , Estresse Oxidativo , Espécies Reativas de Oxigênio/metabolismo
3.
Chemosphere ; 286(Pt 1): 131642, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34351280

RESUMO

Neonicotinoids are replacement insecticides increasingly used for organophosphates, methylcarbamates, and pyrethroids. Experimental evidence suggests neonicotinoids may affect glucose metabolism and insulin secretion through pancreatic ß cell dysfunction, oxidative stress, and inflammation. However, no epidemiologic study has investigated neonicotinoids as potential diabetogens. We examined associations between neonicotinoids with insulin and glucose homeostasis parameters among 1381 non-diabetic adults in the National Health and Nutrition Examination Survey (2015-2016). Urinary concentrations of acetamiprid, clothianidin, imidacloprid, N-desmethyl-acetamiprid, and 5-hydroxy-imidacloprid were quantified. Fasting plasma glucose, insulin, and hemoglobin A1c (HbA1c) were assessed. Insulin resistance was defined as a homeostatic model assessment of insulin resistance ≥2.5. We used weighted linear and logistic regression to estimate associations between detectable neonicotinoids with insulin and glucose homeostasis parameters compared to non-detectable neonicotinoid concentrations. Weighted detection frequencies for imidacloprid, 5-hydroxy-imidacloprid, and N-desmethyl-acetamiprid were 4.4 %, 21.5 %, and 32.8 %, respectively. Detectable imidacloprid (ß = -4.7 µIU/mL, 95 % confidence interval [CI] -8.5, -0.8) and 5-hydroxy-imidacloprid (ß = -2.4 µIU/mL, 95 % CI -4.6, -0.2) were associated with lower fasting plasma insulin levels. Individuals with detectable 5-hydroxy-imidacloprid had lower odds of insulin resistance (odds ratio [OR] = 0.3, 95 % CI 0.2, 0.7). We observed evidence of sexually dimorphic associations between N-desmethyl-acetamiprid with glucose (pint = 0.079) and 5-hydroxy-imidacloprid with HbA1c (pint = 0.038), with patterns suggesting positive associations in males and negative associations in females. Associations between 5-hydroxy-imidacloprid and insulin were modified by body mass index (BMI) (pint = 0.013). We additionally observed age modified associations between 5-hydyroxy-imidacloprid and glucose (pint = 0.048). Results suggest neonicotinoids may be associated with insulin and glucose homeostasis indices and call for prospective studies to examine the metabolic impact of these replacement insecticides in humans.


Assuntos
Inseticidas , Insulina , Adulto , Feminino , Glucose , Homeostase , Humanos , Inseticidas/toxicidade , Masculino , Neonicotinoides , Nitrocompostos , Inquéritos Nutricionais , Estudos Prospectivos
4.
J Sci Food Agric ; 102(1): 434-444, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34143895

RESUMO

BACKGROUND: The incidence of inflammatory bowel disease (IBD) continues to increase worldwide. Multiple factors, including diet, loss of the intestinal barrier function, and imbalance of the immune system can cause IBD. A balanced diet is important for maintaining a healthy bowel and preventing IBD from occurring. The effects of probiotic Lactobacillus gasseri-fermented Maillard reaction products (MRPs) prepared by reacting whey protein with galactose on anti-inflammation and intestinal homeostasis were investigated in this study, which compared MPRs and probiotics separately. RESULTS: In an animal colitis model induced by 2% dextran sulfate sodium (DSS), FWG administration alleviated colon length loss and maintained intestinal immune system homeostasis as reflected by down-regulated interleukin (IL)-6, IL-10, tumor necrosis factor (TNF)-α output, and metallopeptidase-9, and epithelial barrier balance as reflected by up-regulated occludin, E-cadherin, and zonula occludens-1 production in the colon. Furthermore, the expression of splenic cytokines such as IL-6, TNF-α, and IL-10 was up-regulated in the FWG-treated mice in a comparable amount to the control group to ensure the balance of immune responses. CONCLUSION: This study showed that the use of FWG protects the intestines from colitis caused by DSS and maintains immune balance. FWG increased antioxidant enzyme activity, increased intestinal permeability, and regulated the balance of pro- and anti-inflammatory cytokines in the intestines and spleen. Continued intake of FWG can alleviate IBD symptoms through the preservation of mucosal immune responses, epithelial junction and homeostasis through the regulated splenic cytokines. © 2021 Society of Chemical Industry.


Assuntos
Colite/tratamento farmacológico , Produtos Finais de Glicação Avançada/administração & dosagem , Lactobacillus gasseri/metabolismo , Probióticos/administração & dosagem , Animais , Anti-Inflamatórios/administração & dosagem , Colite/induzido quimicamente , Colite/imunologia , Colite/fisiopatologia , Colo/efeitos dos fármacos , Colo/imunologia , Sulfato de Dextrana/efeitos adversos , Modelos Animais de Doenças , Galactose/metabolismo , Produtos Finais de Glicação Avançada/metabolismo , Homeostase/efeitos dos fármacos , Humanos , Interleucina-10/genética , Interleucina-10/imunologia , Interleucina-6/genética , Interleucina-6/imunologia , Mucosa Intestinal/efeitos dos fármacos , Mucosa Intestinal/imunologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Junções Íntimas/genética , Junções Íntimas/imunologia , Fator de Necrose Tumoral alfa/genética , Fator de Necrose Tumoral alfa/imunologia , Proteínas do Soro do Leite/metabolismo
5.
Biol Trace Elem Res ; 200(1): 164-171, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33661473

RESUMO

Dietary cholesterol causes atherosclerosis along with a reduction of copper concentrations in the atherosclerosis wall. This study was to determine the relationship between aorta copper concentrations and the severity of atherosclerotic lesions as well as copper homeostasis in multiple organs in cholesterol-fed rabbits. Male New Zealand white rabbits, 10-week-old and averaged 2.0 kg, were fed a diet containing 1% (w/w) cholesterol or the same diet without cholesterol as controls. Twelve weeks after the feeding, aortic atherosclerotic lesions, serum cholesterol, and multiple organ copper concentrations were measured. Compared to controls, rabbits fed cholesterol-supplemented diet displayed higher serum cholesterol levels and developed atherosclerosis. Copper concentrations in the cholesterol-fed rabbits were increased in the serum and kidney but decreased in the atherosclerosis wall and multiple organs, including heart, liver, spleen, and lung. Furthermore, aorta copper concentrations negatively correlated, respectively, with the severity of the atherosclerotic lesion (r = - 0.64, p = 0.01), the microscope atherosclerotic lesion area (r = - 0.60, p = 0.02), and the stenosis of the lumen (r = - 0.54, p = 0.04). Dietary cholesterol not only causes atherosclerosis but also disturbs copper homeostasis in multiple organ systems. The negative correlation between aorta copper concentrations and the severity of atherosclerotic lesions suggests a vicious cycle between copper reduction and the pathogenesis of atherosclerosis. These changes in copper homeostasis would be additive to atherosclerosis as a risk factor for cardiovascular disease in humans.


Assuntos
Aterosclerose , Colesterol na Dieta , Animais , Aorta , Aterosclerose/induzido quimicamente , Cobre , Homeostase , Masculino , Coelhos
6.
Ann Anat ; 239: 151836, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34563672

RESUMO

Soy isoflavone genistein interplays with numerous physiological or pathophysiological processes during ageing. However, its protective role and underlying mechanisms of action in the regulation of calcium (Ca2+) and phosphate (Pi) homeostasis in an animal model of the andropause are yet to be fully clarified. Wistar male rats (16-month-old) were divided into sham-operated, orchidectomized, orchidectomized estradiol-treated (0.625 mg/kg b.m./day) and orchidectomized genistein-treated (30 mg/kg b.m./day) groups. Treatments were administered subcutaneously for 3 weeks, while the controls received vehicle alone. Estradiol treatment increased the expression level of fibroblast growth factor receptor (FGFR) and parathyroid hormone 1 receptor (PTH1R), and activated mitogen - activated protein kinase kinase 1/2 (MEK 1/2) signaling pathway in the kidneys. Genistein application induced a prominent gene and protein expression of Klotho and downregulated the expression of FGFR and PTH1R in the kidney of andropausal rats. Activation of protein kinase B (Akt) signalling pathway was observed, while MEK 1/2 signaling pathway wasn't altered after genistein treatment. The increase of 25 (OH) vitamin D in the serum and decrease in Ca2+ urine content was observed after genistein application. Our findings strongly suggest genistein as a potent biocompound with beneficial effects on the regulation of Ca2+ and Pi homeostasis, especially during aging process when the balance of mineral metabolism is impaired. These novel data provide closer insights into the physiological roles of genistein in the regulation of mineral homeostasis.


Assuntos
Andropausa , Genisteína , Animais , Cálcio , Modelos Animais de Doenças , Genisteína/farmacologia , Homeostase , Masculino , Quinases de Proteína Quinase Ativadas por Mitógeno , Orquiectomia , Fosfatos , Ratos , Ratos Wistar , Transdução de Sinais
7.
Chemosphere ; 286(Pt 3): 131827, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34403897

RESUMO

Precision farming using nanoparticles is a cutting-edge technology for safe cultivation of crop plants in marginal areas afflicted with environmental/climatic stresses like salinity, drought, extremes of temperature, ultraviolet B stress or polluted with xenobiotics like toxic heavy metals and fluoride. Major cereal crops like rice, wheat, maize, barley, sorghum and millets which provide the staple food for the entire global population are mainly glycophytes and are extremely susceptible to abiotic stress-induced oxidative injuries. Nanofertilization/exogenous spraying of beneficial nanoparticles alleviates the oxidative damages in cereals by altering the homeostasis of phytohormones like abscisic acid, gibberellins, cytokinins, auxins, salicylic acid, jasmonic acid and melatonin and by triggering the synthesis of gasotransmitter nitric oxide. Signaling cross-talks of nanoparticles with plant growth regulators enable activation of the defence machinery, comprising of antioxidants, thiol-rich compounds and glyoxalases and restrict xenobiotic mobilization by suppressing the expression of associated transporters. Accelerated nutrient uptake and grain biofortification under the influence of nanoparticles result in optimum crop productivity under sub-optimal conditions. However, over-dosing of even beneficial nanoparticles promotes severe phytotoxicity. Hence, the concentration of nanoparticles and mode of administering need to be thoroughly standardized before large-scale field applications, to ensure sustainable cereal cultivation with minimum ecological imbalance.


Assuntos
Grão Comestível , Nanopartículas , Homeostase , Nanopartículas/toxicidade , Nutrientes , Estresse Fisiológico , Xenobióticos/toxicidade
8.
Sci Total Environ ; 805: 150360, 2022 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-34818773

RESUMO

Previous studies have suggested that perfluoroalkyl substances (PFASs) can act as endocrine disruptors, but few studies have investigated the effects of serum PFASs on estrogen homeostasis during pregnancy. The present study included 557 pregnant women in Tangshan City, North China, and determined 11 serum PFASs in the early term of pregnancy and three typical estrogens (estrone (E1), estradiol (E2) and estriol (E3)) in the early (n = 557), middle (n = 339), and late (n = 286) terms of pregnancy. Sociodemographic factors and diet information were obtained by structured questionnaires. After adjusting for potential confounders, multiple linear regression model demonstrated negative associations of natural logarithmic transformed serum perfluoroundecanoic acid (Ln PFUdA) with Ln E1and Ln E3 in the early term of pregnancy with ß coefficients of -0.060 (95% confidence interval (CI): -0.101 to -0.019) and -0.041 (95% CI: -0.070 to -0.011), respectively. Ln perfluorodecanoic acid (PFDA) was negatively associated with averaged E1 in the early and middle (EM) terms of pregnancy with a ß coefficient of -0.205 (95% CI: -0.357 to -0.053). Ln perfluorononanoic acid (PFNA) tended to be negatively associated with E2 in the late term of pregnancy with a ß coefficient of -0.134 (95% CI: -0.253 to -0.016) although p-value was slightly greater than 0.05 after false discovery rate (FDR) correction. Mixed effect model found that serum PFDA was negatively associated with E1 (ß = -0.123, 95% CI: -0.235 to -0.012) during the entire pregnancy. These findings suggested that exposure to PFASs disturbed estrogen homeostasis in pregnant women and the effects varied with the terms of pregnancy.


Assuntos
Ácidos Alcanossulfônicos , Poluentes Ambientais , Fluorcarbonetos , Estrogênios , Feminino , Homeostase , Humanos , Gravidez , Gestantes
9.
Adv Exp Med Biol ; 1354: 127-143, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-34807440

RESUMO

Amino acids (AAs) not only serve as building blocks for protein synthesis in microorganisms but also play important roles in their metabolism, survival, inter-species crosstalk, and virulence. Different AAs have their distinct functions in microbes of the digestive tract and this in turn has important impacts on host nutrition and physiology. Deconjugation and re-conjugation of glycine- or taurine- conjugated bile acids in the process of their enterohepatic recycling is a good example of the bacterial adaptation to harsh gut niches, inter-kingdom cross-talk with AA metabolism, and cell signaling as the critical control point. It is also a big challenge for scientists to modulate the homeostasis of the pools of AAs and their metabolites in the digestive tract with the aim to improve nutrition and regulate AA metabolism related to anti-virulence reactions. Diversity of the metabolic pathways of AAs and their multi-functions in modulating bacterial growth and survival in the digestive tract should be taken into consideration in recommending nutrient requirements for animals. Thus, the concept of functional amino acids can guide not only microbiological studies but also nutritional and physiological investigations. Cutting edge discoveries in this research area will help to better understand the mechanisms responsible for host-microbe interactions and develop new strategies for improving the nutrition, health, and well-being of both animals and humans.


Assuntos
Aminoácidos , Estado Nutricional , Animais , Bactérias , Ácidos e Sais Biliares , Homeostase , Humanos , Necessidades Nutricionais
10.
Chemosphere ; 287(Pt 3): 132332, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34563771

RESUMO

Nanotechnology has become a valuable novel approach to manage several environmental challenges through providing innovative and effective solutions. Heavy metal stress is an important abiotic limiting factor. Seed priming with selenium (Se) alleviates various kinds of environmental stresses; yet, the potential of seed priming with selenium nanoparticles (SeNPs) under cadmium (Cd) stress for coriander crop has never been evaluated. This research work was designed to explore the effects of seed priming with three levels (0, 5, 10 and 15 mg L-1) of SeNPs solution on the physio-biochemical characteristics, nutrition, antioxidative defense system and growth of coriander under Cd stress. Cadmium toxicity reduced chlorophyll content, photosynthetic activity and growth of treated plants. Moreover, Cd stressed plants exhibited modulations in proline level, together with decreased water potential, and leaf osmotic potential. However, SeNPs increased growth attributes, chlorophyll content, total soluble sugars, leaf relative water content, and gas exchange parameters in treated plants which were conversely decreased by Cd toxicity. The seeds priming with SeNPs promoted antioxidant response by increasing catalase (CAT), ascorbate peroxidase (APX) and peroxidase (POX) activity and safeguarding cellular structures through scavenging free radicals and reactive oxygen species. Furthermore, Cd stressed plants displayed an upper level of MDA (1.91 fold) while SeNPs improved membranous integrity through detoxification of hydrogen peroxide. Additionally, SeNPs enhanced nutrients contents (P, K, Ca, Mg, Zn), metal tolerance index and diminished Cd content in plants resulting in the improved growth and development of Cd affected coriander plants.


Assuntos
Coriandrum , Nanopartículas , Selênio , Antioxidantes , Cádmio/toxicidade , Homeostase , Peróxido de Hidrogênio , Nanopartículas/toxicidade , Estresse Oxidativo
11.
An Acad Bras Cienc ; 93(suppl 4): e20210088, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34730622

RESUMO

This study aimed to evaluate the effects of the dietary supplementation Curcuma longa hydrolate on Nile tilapia (Oreochromis niloticus) reared in a recirculation system. Hemato-immunological parameters, growth performance, nitrogen and phosphorus retention, as well as body composition and its interaction with the intestinal microbiota, were studied. Nile tilapia fingerlings (120) were distributed randomly in 8 polyethylene tanks (40 L). The experimental units were divided into two treatments, in quadruplicate: commercial diet supplemented with 2.5% of C. longa hydrolate and commercial diet without supplementation (control). After 45 days, the treatment supplemented hydrolate showed higher survival than the control group, 95.25% and 82.22%, receptively. In the blood profile, fish supplemented with hydrolate had a higher count of total leukocytes and neutrophils, as well as mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration, than control group. The hydrolate group showed a substantial increase in the relative abundance of Cetobacterium and Romboutsia, as well as lower diversity in gut microbiota. The dietary addition of C. longa hydrolate for Nile tilapia seems to have a beneficial effect on gut microbiota, in addition to a likely positive effect on the physiological performance of Nile tilapia by maintaining intestinal homeostasis and promoting survival in reared conditions.


Assuntos
Ração Animal , Ciclídeos , Microbiota , Ração Animal/análise , Animais , Curcuma , Dieta/veterinária , Suplementos Nutricionais , Homeostase
12.
Plant Sci ; 313: 111087, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34763872

RESUMO

Four members of phosphoinositide-specific phospholipase C (PI-PLC) are predicted in rice genome. Although the involvement of OsPLC1 and OsPLC4 in the responses of rice to salt and drought stresses has been documented, the role of OsPLC3 in which, yet, is elusive. Here, we report that OsPLC3 was ubiquitously expressed in various tissues during the development of rice. The expression of YFP-tagged OsPLC3 was observed at the plasma membrane (PM), cytoplasm and nucleus of rice protoplasts, onion epidermal cells and tobacco leaves. The catalytic activity of OsPLC3 was measured using the thin-layer chromatography (TLC) method. The inhibition of OsPLC3 expression was detected in the treatments of NaCl and mannitol. Overexpression (OE) of OsPLC3 produced plants showing more sensitive to osmotic stresses when they were compared to the wild-type (HJ) and osplc3 mutants, the phenomena such as decreased plant fresh weight and increased water loss rate (WLR) were observed. Under the treatment of NaCl or mannitol, expressions of a subset osmotic stress-related genes were altered, in both OE and osplc3 mutant lines. In addition, the expressions and the enzyme activities of reactive oxygen species (ROS) scavengers were significantly decreased in OE lines, leading to over-accumulation of ROS together with less osmotic adjustment substances including proline, soluble sugars and soluble proteins in OE plants which caused the growth inhibition. Thus, our results suggested that, via modulating ROS homeostasis, OsPLC3 is involved in responses to the osmotic stress in rice.


Assuntos
Homeostase/fisiologia , Oryza/crescimento & desenvolvimento , Oryza/genética , Oryza/metabolismo , Pressão Osmótica/fisiologia , Fosfatidilinositóis/metabolismo , Fosfolipases/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Produtos Agrícolas/genética , Produtos Agrícolas/crescimento & desenvolvimento , Produtos Agrícolas/metabolismo , Fosfatidilinositóis/genética , Fosfolipases/genética
13.
Scand J Immunol ; 94(4): e13094, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34780092

RESUMO

The thymus produces self-limiting and self-tolerant T cells through the interaction between thymocytes and thymus epithelial cells (TECs), thereby generating central immune tolerance. The TECs are composed of cortical and medullary thymic epithelial cells, which regulate the positive and negative selection of T cells, respectively. During the process of negative selection, thymocytes with self-reactive ability are deleted or differentiated into regulatory T cells (Tregs). Tregs are a subset of suppressor T cells that are important for maintaining immune homeostasis. The differentiation and development of Tregs depend on the development of TECs and other underlying molecular mechanisms. Tregs regulated by thymic epithelial cells are closely related to human health and are significant in autoimmune diseases, thymoma and pregnancy. In this review, we summarize the current molecular and transcriptional regulatory mechanisms by which TECs affect the development and function of thymic Tregs. We also review the pathophysiological models of thymic epithelial cells regulating thymic Tregs in human diseases and specific physiological conditions.


Assuntos
Linfócitos T Reguladores/citologia , Linfócitos T Reguladores/imunologia , Imunidade Adaptativa , Animais , Doenças Autoimunes/genética , Doenças Autoimunes/imunologia , Diferenciação Celular/imunologia , Células Dendríticas/citologia , Células Dendríticas/imunologia , Células Epiteliais/classificação , Células Epiteliais/citologia , Células Epiteliais/imunologia , Feminino , Homeostase , Humanos , Masculino , Modelos Imunológicos , Poliendocrinopatias Autoimunes/genética , Poliendocrinopatias Autoimunes/imunologia , Gravidez , Transdução de Sinais/imunologia , Linfócitos T Reguladores/classificação , Timócitos/classificação , Timócitos/citologia , Timócitos/imunologia , Timoma/imunologia , Timo/citologia , Timo/imunologia , Neoplasias do Timo/imunologia
14.
Pestic Biochem Physiol ; 179: 104961, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34802511

RESUMO

As a typical glycolytic inhibitor, 3-bromopyruvate (3-BrPA) has been extensively studied in cancer therapy in recent decades. However, few studies focused on 3-BrPA in regulating the growth and development of insects, and the relationship and regulatory mechanism between glycolysis and chitin biosynthesis remain largely unknown. The Hyphantria cunea, named fall webworm, is a notorious defoliator, which caused a huge economic loss to agriculture and forestry. Here, we investigated the effects of 3-BrPA on the growth and development, glycolysis, carbohydrate homeostasis, as well as chitin synthesis in H. cunea larvae. To elucidate the action mechanism of 3-BrPA on H. cunea will provide a new insight for the control of this pest. The results showed that 3-BrPA dramatically restrained the growth and development of H. cunea larvae and resulted in larval lethality. Meanwhile, we confirmed that 3-BrPA caused a significant decrease in carbohydrate, adenosine triphosphate (ATP), pyruvic acid (PA), and triglyceride (TG) levels by inhibiting glycolysis in H. cunea larvae. Further studies indicated that 3-BrPA significantly affected the activities of hexokinase (HK), phosphofructokinase (PFK), pyruvate kinase (PK), glucose 6-phosphate dehydrogenase (G6PDH) and trehalase, as well as expressions of the genes related to glycolysis, resulting in carbohydrate homeostasis disorder. Moreover, it was found that 3-BrPA enhanced 20-hydroxyecdysone (20E) signaling by upregulating HcCYP306A1 and HcCYP314A1, two critical genes in 20E synthesis pathway, and accelerated chitin synthesis by upregulating transcriptional levels of genes in the chitin synthesis pathway in H. cunea larvae. Taken together, our findings provide a novel insight into the mechanism of glycolytic inhibitor in regulating the growth and development of insects, and lay a foundation for the potential application of glycolytic inhibitors in pest control as well.


Assuntos
Carboidratos , Glicólise , Animais , Homeostase , Larva , Piruvatos
15.
Pestic Biochem Physiol ; 179: 104974, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34802524

RESUMO

Folpet, a fungicide, is utilized even in cosmetics and pharmaceuticals. The LD50 of folpet in mammals, birds, and fish is relatively high. Recently, several negative effects of folpet on the respiratory system and cornea have been reported. However, there is no study on the negative effects of folpet on maternal-fetus interactions. In the present study, we used porcine trophectoderm (pTr) cells and porcine luminal epithelial (pLE) cells to investigate the toxic effects of folpet during implantation. Folpet treatment decreased cell proliferation and promoted apoptosis with cell cycle arrest. In addition, the ERK, JNK, and AKT signal pathways were activated by folpet treatment. Folpet treatment induced calcium overload in pTr and pLE cells mediating antimigratory and antiadhesive effects in both cell lines. Co-treatment with calcium chelates decreased the anti-implantation effect of folpet. Overall, our results demonstrated potential reproductive toxicity of folpet in pig.


Assuntos
Cálcio , Fosfatidilinositol 3-Quinases , Animais , Células Epiteliais , Homeostase , Ftalimidas , Gravidez , Suínos
16.
Rev Assoc Med Bras (1992) ; 67(7): 1026-1032, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34817518

RESUMO

OBJECTIVE: Many chronic diseases such as malignancy, cardiovascular diseases, endothelial dysfunction, and autoimmune diseases, which have been shown to be related to vitamin D in various studies; have similar relations with CTRP-9, TNFα, and thiol-disulfide hemostasis. We aimed to contribute to the literature by evaluating the relationship between CTRP-9, TNFα, and thiol-disulfide hemostasis and vitamin D levels, which we thought may have some effects on the pathogenesis of vitamin D deficiency. METHODS: In our study, 78 female volunteers older than 18 years were included. Volunteers were divided into three groups according to the reference values of vitamin D levels. Biochemical parameters, CTRP-9, TNFα, and thiol/disulfide hemostasis tests taken from all volunteers were studied. RESULTS: In this study, there was a significant difference in CTRP-9, TNFα, total thiol (TT), native thiol (NT), DIS (disulfide), TT/DIS, and NT/DIS levels in vitamin D groups (p<0.05). There was a significant negative correlation between vitamin D and TNFα and DIS, while a significant positive correlation was found with CTRP-9, TT, NT, TT/DIS, and NT/DIS (p<0.05). CONCLUSIONS: It was determined that vitamin D deficiency causes a significant decrease in CTRP-9 level and a significant increase in TNFα level, as well as an increase in thiol/disulfide hemostasis in favor of disulfide, which may be a risk factor for increased oxidative stress. We considered that these changes may play mediator roles for many chronic diseases and metabolic disorders that are increasing in frequency due to vitamin D deficiency.


Assuntos
Dissulfetos , Fator de Necrose Tumoral alfa , Biomarcadores , Feminino , Hemostasia , Homeostase , Humanos , Estresse Oxidativo , Compostos de Sulfidrila , Vitamina D
17.
Adv Exp Med Biol ; 1348: 273-309, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34807425

RESUMO

Cutis laxa (CL) syndromes are a large and heterogeneous group of rare connective tissue disorders that share loose redundant skin as a hallmark clinical feature, which reflects dermal elastic fiber fragmentation. Both acquired and congenital-Mendelian- forms exist. Acquired forms are progressive and often preceded by inflammatory triggers in the skin, but may show systemic elastolysis. Mendelian forms are often pleiotropic in nature and classified upon systemic manifestations and mode of inheritance. Though impaired elastogenesis is a common denominator in all Mendelian forms of CL, the underlying gene defects are diverse and affect structural components of the elastic fiber or impair metabolic pathways interfering with cellular trafficking, proline synthesis, or mitochondrial functioning. In this chapter we provide a detailed overview of the clinical and molecular characteristics of the different cutis laxa types and review the latest insights on elastic fiber assembly and homeostasis from both human and animal studies.


Assuntos
Cútis Laxa , Animais , Cútis Laxa/genética , Tecido Elástico , Homeostase , Humanos , Redes e Vias Metabólicas , Síndrome
18.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 29(5): 1380-1386, 2021 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-34627414

RESUMO

OBJECTIVE: To investigate the effect of autophagy to the ferroptosis in acute lymphocytic leukemia (ALL) cells and its mechanism. METHODS: ALL cell lines (including Reh, Jurkat and CCRF-CEM) were selected. The cell viability was detected by MTS assay and trypan blue staining was used to evaluate the death of the cell. The expression of autophagy related protein (including p62, LC3I/II) and Ferritin in ALL cells were detected by Western blot. The alteration of labile iron pool (LIP) in ALL cells was evaluated by flow cytometry. RESULTS: Reh cells showed sensitivity to ferroptosis activator Erastin, while Jurkat and CCRF-CEM cells showed resistant. Autophagy activator rapamycin could promote the sensitivity of Jurkat and CCRF-CEM cells to Erastin, and the ferroptosis of the cells (P<0.001). Autophagy inhibitor chloroquine could reduce the sensitivity of Reh cells to Erastin and resist the ferroptosis of the cells (P<0.001). The expression of Ferritin could be down-regulated after autophagy was activated in Jurkat and CCRF-CEM cells (P<0.05), while the level of LIP was significantly increased (P<0.05). Inhibiting the autophgy in Reh cells could up-regulate the expression of Ferritin (P<0.01),while decrease the level of LIP (P<0.001). CONCLUSION: The iron homeostasis in cells could be regulated by autophagy through affecting Ferritin expression and LIP level. Autophagy can alter sensitivity of ALL cells to ferroptosis activator Erastin, which suggestes that combining autophagy regulator with ferroptosis activator may be a new strategy for the treatment of chemotherapy-resistant ALL.


Assuntos
Ferroptose , Leucemia-Linfoma Linfoblástico de Células Precursoras , Autofagia , Homeostase , Humanos , Ferro , Espécies Reativas de Oxigênio
19.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 29(5): 1623-1630, 2021 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-34627451

RESUMO

OBJECTIVE: To investigate the effect of lysosomal-associated protein transmembrane-4 Beta(Laptm4b) deletion on hematopoietic stem/progenitor cells (HSPCs) homeostasis in mice. METHODS: The hematopoietic system specific Laptm4b-deficient mice were constructed. The number and proportion of HSPCs (LSK, LT, ST, MPP, etc) in Laptm4b-deficient mice were analyzed by flow cytometry. Single SLAM-HSC cell was sorted by flow sorter and cultured in vitro to measure the effect of Laptm4b deletion on the colony forming ability of hematopoietic stem cells (HSCs). The effect of Laptm4b-deficient on the reconstitution ability of HSCs in mice was detected by competitive transplantation experiment of SLAM-HSC cells. RESULTS: Laptm4b deficiency could moderately upregulate the proportion of T cells in the peripheral blood of the mice, but showed no significant effect on the proportion and number of HSPCs. Laptm4b deletion showed no effect on the reconstruction ability of HSCs after competitive transplantation, but it could inhibit the colony formation of HSCs in vitro. CONCLUSION: LAPTM4B may play a role in HSCs under the proliferation stress. Laptm4b-deficient in mice hematopoietic system showed no significant effect on the HSPCs homeostasis maintenance and reconstruction ability.


Assuntos
Células-Tronco Hematopoéticas , Fatores de Transcrição , Animais , Proliferação de Células , Citometria de Fluxo , Homeostase , Camundongos
20.
Front Cell Infect Microbiol ; 11: 696554, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34595127

RESUMO

The circadian clock regulates numerous key physiological processes and maintains cellular, tissue, and systemic homeostasis. Disruption of circadian clock machinery influences key activities involved in immune response and brain function. Moreover, Immune activation has been closely linked to neurodegeneration. Here, we review the molecular clock machinery and the diurnal variation of immune activity. We summarize the circadian control of immunity in both central and peripheral immune cells, as well as the circadian regulation of brain cells that are implicated in neurodegeneration. We explore the important role of systemic inflammation on neurodegeneration. The circadian clock modulates cellular metabolism, which could be a mechanism underlying circadian control. We also discuss the circadian interventions implicated in inflammation and neurodegeneration. Targeting circadian clocks could be a potential strategy for the prevention and treatment of inflammation and neurodegenerative diseases.


Assuntos
Relógios Circadianos , Encéfalo , Ritmo Circadiano , Homeostase , Humanos , Inflamação
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