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1.
Ann Agric Environ Med ; 28(3): 414-418, 2021 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-34558263

RESUMO

INTRODUCTION: Fusaria are microscopic filamentous fungi which are spread in soil, in various organic substrates, and include more than 80 phytopathogenic species which are predominantly hosted by cereals, fruits and vegetables. Many of these species, under certain conditions, are capable of synthesizing secondary metabolites, mycotoxins. At present, various substances are used for their elimination and one of the solutions appears to be essential oils. In the presented study, the antifungal activity of essential oils was researched in vitro. MATERIAL AND METHODS: In this study, two standard fungal isolates Fusarium graminearum CCM F-683 and Fusarium graminearum CCM 8244 (Brno, Czech Republic) were used. The antifungal effect of 6 tested essential oils (Syzygium aromaticum, Origanum vulgare, Thymus vulgaris, Hyssopus officinalis , Ocimum basilicum, Myristica fragrans) was determined using the broth microdilution method, which allows reading of the MIC (minimum inhibitory concentration). According to the results obtained, the growth inhibition of Fusarium graminearum was determined by assay for the inhibition of radial growth of the mycelium. RESULTS: The inhibitory effects of thymus, oregano, basil, myristica, hyssop and syzygium essential oil (EO) on mycelial growth of Fusarium graminearum CCM F-683 and CCM 8244 were investigated. The best antifungal activity against the both strains of Fusarium graminearum (37.4%; 40.7%) was demonstrated by Origanum vulgare EO at the concentration 100 µg/mL. Among the four tested oils, three (Syzygium aromaticum, Thymus vulgaris, Origanum vulgare) achieved the best inhibitory effect (100%) at concentrations 500 µg/mL and 1000 µg/mL. CONCLUSIONS: In the protection of plants against pathogenic fungi, essential oils appear to be a suitable substitute for synthetic chemicals.


Assuntos
Fungicidas Industriais/farmacologia , Fusarium/efeitos dos fármacos , Óleos Voláteis/farmacologia , Óleos Vegetais/farmacologia , Fusarium/crescimento & desenvolvimento , Hyssopus/química , Testes de Sensibilidade Microbiana , Myristica/química , Ocimum basilicum/química , Origanum/química , Syzygium/química , Thymus (Planta)/química
2.
Biol Trace Elem Res ; 199(2): 753-762, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32394355

RESUMO

To investigate the effect of harvest height on the amassed elements and ash content of Hyssop (Hyssopus officinalis L.), an experiment was conducted in a randomized complete block design with three replications. Treatments included four harvest heights, i.e., 15, 25, 35, and 45 cm (from the tip of the plant) and the residual stalks. The dependent variables were the amassed content of elements N, K, P, Ca, Mg, Cu, Zn, and Pb in different heights of the plant and the associated ash content (total ash (TA), acid-insoluble ash (AA), and water-insoluble ash (WA)). The results showed that by moving from the upper shoots toward the ground, the amassed content of Mg, Ca, Cu, Zn, and Pb increased by 22.67%, 43.74%, 12.87%, 39.02%, and 85.04%, respectively. Further, a downward trend was observed for N (50.16%) and K (6.41%) content, while an upward trend reported for P (29.06%) content. As for the residual stalks, by moving from the upper shoots toward the ground, Mg, Ca, Cu, Zn, and Pb contents decreased by 1.01%, 21.03%, 9.11%, 17.02%, and 51.06%, respectively, while N and P contents increased by 60.59% and 3.15%, respectively, and a 34.74% increase was seen in P content. With increasing harvest height, TA, AA, and WA values increased by 33.48%, 27.03%, and 18.25%, respectively. As for the residues, these variables increased by 11.44%, 6.35%, and 5.22%, respectively. Our results showed that 15 cm harvest height had the highest quality with the lowest heavy metal content. Graphical Abstract.


Assuntos
Metais Pesados , Plantas Medicinais , Hyssopus
3.
BMC Complement Med Ther ; 20(1): 228, 2020 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-32689984

RESUMO

BACKGROUND: Hyssopus cuspidatus Boriss has been used as an important ethnomedicinal plant for long to eliminate phlegm, relieve cough and as well as having antibacterial, antioxygenation, and antitumor activities. In this study, the polyphenol contents, flavonoid contents, free radical scavenging assay and animal antioxygenation property assay of ethanol extract of H. cuspidatus were measured. METHODS: This study determined the total polyphenol and flavonoid contents in H. cuspidatus by UV-VIS. Caffeic, ferulic, and rosmarinic acids were measured using HPLC-DAD. Free radical scavenging assay of H. cuspidatus was studied by colorimetric method. Animal antioxygenation property assay of H. cuspidatus was studied with mice by biochemical assay kits. RESULTS: The total polyphenol and flavonoid contents of H. cuspidatus in 2017, 2018, 2019 were determined and the contents of H. cuspidatus in 2019 was the highest. In addition, rosmarinic acid was the phenolic acid with the highest content in H. cuspidatus. Compared with those of DPPH free radical, hydroxyl free radical, and superoxide anion free radical, the scavenging ability of H. cuspidatus of ABTS free radical was stronger, the average IC50 value was 0.0245 mg/mL. In animal antioxygenation property experiment, the model group was successfully established with decreased activities of SOD, CAT, and GSH-px and increased content of MDA. The ethanol extract of H. cuspidatus increased the activities of SOD, CAT, and GSH-px and reduced the content of MDA. Each group of samples and the ascorbic acid positive control group showed significant differences in the results of free radical scavenging and animal antioxygenation property experiments (P < 0.05). CONCLUSIONS: These results suggest that H. cuspidatus exerts an antioxygenation property, which can be attributed to the contents of total polyphenol and flavonoid. Given its strong antioxygenation property, H. cuspidatus can be used as a new natural antioxidant in food preservation and disease treatment.


Assuntos
Antioxidantes/química , Flavonoides/química , Sequestradores de Radicais Livres/química , Hyssopus/química , Extratos Vegetais/química , Polifenóis/química , Animais , Ácidos Cafeicos/química , China , Cromatografia Líquida de Alta Pressão , Cinamatos/química , Ácidos Cumáricos/química , Depsídeos/química , Masculino , Camundongos , Estrutura Molecular
4.
Food Chem ; 333: 127433, 2020 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-32659662

RESUMO

This study examined the effects of exogenous citrulline (control, 1 and 2 mM) and water availability (100%, 50% and 25% WA) on antioxidant attributes and essential oil constituents of Hyssopus officinalis L. in two successive harvests. Hyssop tolerantly responded to water deficiency by well-promoted antioxidant enzymes (i.e., superoxide dismutase, and catalase), strong DPPH-scavenging activity, and increasing polyphenols; however, the essential oil content was negatively reduced by water stress. External citrulline further increased the activity of antioxidant enzymes. Citrulline application at 2 mM under severe water stress could also improve essential oil (EO) content in the first and second harvests by about 15 and 30%, respectively. Furthermore, under severe drought, citrulline at 2 mM could obtain the highest yield of isopinocamphone (47%) as the main component of EO. The results showed the high potential of this novel applied metabolite agent to be used in a well-fulfilled production of this medicinal plant.


Assuntos
Antioxidantes/metabolismo , Citrulina/farmacologia , Hyssopus/efeitos dos fármacos , Hyssopus/fisiologia , Óleos Voláteis/metabolismo , Canfanos/metabolismo , Catalase/metabolismo , Catecol Oxidase/metabolismo , Desidratação , Secas , Hyssopus/química , Óleos Voláteis/análise , Proteínas de Plantas/metabolismo , Plantas Medicinais/efeitos dos fármacos , Plantas Medicinais/fisiologia , Polifenóis/metabolismo , Superóxido Dismutase/metabolismo
5.
Bioelectromagnetics ; 41(6): 403-412, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32573004

RESUMO

The present study was carried out in order to evaluate the promoting effect of a static magnetic field (SMF) on drought tolerance and medicinal properties in Hyssopus officinalis. In the current work, the effect of seed priming with SMF (45, 90, 200, and 250 mT for 5 min) was investigated in 60-day-old hyssop (H. officinalis) plants that were irrigated every 8 days. The assessments consisted of total dry mass, membrane integrity, photosynthetic pigment concentrations, polyphenol content, antioxidant enzyme activities, and antioxidant capacity. Compared with exclusively water stress, magnetopriming, particularly at 200 mT, significantly altered these parameters in the grown plants. At this intensity, the level of total dry mass, total chlorophyll, and polyphenol content increased by 94%, 2.5- and 7.7-fold, respectively. Also, the level of electrolyte leakage and malondialdehyde decreased by 35% and 33%. The reducing power, DPPH (1,1-diphenyl-2-picrylhydrozyl), and superoxide anion-scavenging activities were highly augmented as well. Magnetopriming at 200 mT increased catalase (+92%) and ascorbate peroxidase (+2.3-fold) activities. However, the highest activity of guaiacol peroxidase was recorded at 90 mT. Generally, the present study illustrated the positive effect of magnetopriming (200 mT) on improvement of drought tolerance in H. officinalis through protection of cellular membrane integrity, maintenance of photosynthetic pigment content, and alternation of antioxidant enzyme activities. Furthermore, the data showed this treatment (200 mT) not only had no negative effect on medicinal properties of H. officinalis, but also improved it via increasing total phenolic content and antioxidant capacity. Bioelectromagnetics. 2020;41:403-412. © 2020 Bioelectromagnetics Society.


Assuntos
Hyssopus/crescimento & desenvolvimento , Hyssopus/metabolismo , Campos Magnéticos , Compostos Fitoquímicos/metabolismo , Estresse Fisiológico , Água/farmacologia , Antioxidantes/metabolismo , Hyssopus/efeitos dos fármacos , Hyssopus/fisiologia , Fotossíntese
6.
Nat Prod Res ; 34(4): 534-540, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30417654

RESUMO

Eighteen compounds were isolated from the ethyl acetate fraction of the aerial part of Hyssopus cuspidatus. Their structures were established by analysis of mass and NMR data, as well as comparison with previous published data in the literatures. Among them, ten compounds were found from the Hyssopus genus for the first time, and one compound was isolated from H. cuspidatus for the first time. HPLC-DAD-ESI-HRMS/MS investigations was applied to further obtain the phenolic profiling of the ethyl acetate fraction, and nine derivatives of caffeic acid and ferulic or isoferulic acid were identified. Antioxidant activity against DPPH free radical and antibacterial activity against Candida albicans, Escherichia coli and Staphylococcus aureus were evaluated. The ethyl acetate fraction exhibited weak antioxidant activity and moderate antibacterial activity. The isolated compounds showed weak to potent antioxidant and antibacterial activity. To the best of our knowledge, this is the first report on the antioxidant and antibacterial activity of H. cuspidatus.


Assuntos
Antibacterianos/isolamento & purificação , Antioxidantes/isolamento & purificação , Hyssopus/química , Extratos Vegetais/química , Antibacterianos/química , Antibacterianos/farmacologia , Antioxidantes/química , Antioxidantes/farmacologia , Bactérias/efeitos dos fármacos , Cromatografia Líquida de Alta Pressão/métodos , Espectrometria de Massas/métodos , Fenóis/análise
7.
Andrologia ; 52(1): e13450, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31692026

RESUMO

The unclear bio-safety issue and potential risk of nanoparticles (NPs) on various organelles can be considered as a major challenge. In the present study, we have assessed the green synthesis of ZnO nanoparticles using Hyssop (Hyssopus officinalis) extract and their effects on PC3 cell line and BALB/c mice model. The cytotoxicity of the ZnO-NPs was assessed on PC3 cell line by MTT test after characterisation. Apoptotic effect of ZnO-NPs was determined by in vitro AO/PI staining. The histopathological assessments and determination of LH and FSH levels carried out as in vivo analysis in BALB/c adult male mice. The expression of major genes involved in spermatogenesis and sperm maturation (Adam3, Prm1, Spata19, Tnp2, Gpx5) were also analysed. The obtained result demonstrated that the IC50 for PC3 cell line treated with green-synthesised ZnO-NPs during 24 and 48 hr was reported 8.07 and 5 µg/ml respectively. Meanwhile, the induced apoptosis was recorded 26.6% ± 0.05, 44% ± 0.12 and 80% ± 0.07 of PC3 cells. The results of gene expression analysis revealed that the increase in the concentration of ZnO-NPs significantly (p < .05) down-regulated the Adam3, Prm1, Spata-19, Tnp2 and Gpx5 genes. The overall results of this research elucidated that ZnO-NPs impaired spermatogenesis, sperm maturation process and sperm motility.


Assuntos
Nanopartículas Metálicas/efeitos adversos , Neoplasias da Próstata/tratamento farmacológico , Espermatogênese/efeitos dos fármacos , Testículo/efeitos dos fármacos , Óxido de Zinco/efeitos adversos , Administração Oral , Animais , Apoptose/efeitos dos fármacos , Linhagem Celular Tumoral , Ensaios de Seleção de Medicamentos Antitumorais , Química Verde/métodos , Humanos , Hyssopus/química , Concentração Inibidora 50 , Masculino , Nanopartículas Metálicas/administração & dosagem , Nanopartículas Metálicas/química , Camundongos , Camundongos Endogâmicos BALB C , Extratos Vegetais/química , Folhas de Planta/química , Próstata/citologia , Próstata/efeitos dos fármacos , Neoplasias da Próstata/patologia , Motilidade Espermática/efeitos dos fármacos , Testículo/patologia , Testes de Toxicidade Subaguda , Óxido de Zinco/administração & dosagem , Óxido de Zinco/síntese química
8.
Biomolecules ; 9(12)2019 12 11.
Artigo em Inglês | MEDLINE | ID: mdl-31835857

RESUMO

This study aimed at determining the effect of aqueous ethanolic extracts from lemon balm, hyssop and nettle, and butylated hydroxyanisole (BHA) on properties of shortbread cookies. This was achieved by instrumental measurements of color and sensory properties of the cookies directly after baking and by determination of peroxide (PV) and p-anisidine (p-AnV) values, and specific extinction coefficients (K232 and K268 values) for fat extracted from the cookies stored for 3 months at room temperature. Increase of the herbal extracts' concentration from 0.02% to 0.2% in the cookies caused a reduction of L* (the brightness) and a* values (the red coordinate), while b* values (the yellow coordinate) increased when the cookies were enriched with lemon balm and nettle extracts. Among the cookies studied, those prepared with BHA and 0.1 and 0.2% addition of lemon balm extracts were characterized by the highest scores for aroma, taste, and overall acceptability. Incorporation of BHA and 0.02% hyssop extract into the cookies caused a decrease of PV values (the peroxide value) for fat extracted from the cookies after 3 months of their storage compared to a (control) sample without additives and produced the lowest K232 values. Changes in the p-AnV values for the fat samples studied occurred gradually and slowly during the storage and the obtained values were lower compared to the control sample. All of the studied fat samples also showed a higher ability to scavenge DPPH radicals than the control sample. Considering both PV and p-AnV values as indicators of fat oxidation, BHA protected fat extracted from cookies against oxidation better than the herbal extracts used.


Assuntos
Cor , Gorduras na Dieta/análise , Alimentos/normas , Sequestradores de Radicais Livres/química , Extratos Vegetais/química , Plantas Medicinais/química , Compostos de Bifenilo/antagonistas & inibidores , Hidroxianisol Butilado/química , Sequestradores de Radicais Livres/farmacologia , Hyssopus/química , Melissa/química , Picratos/antagonistas & inibidores , Extratos Vegetais/farmacologia , Urtica dioica/química
9.
Molecules ; 24(22)2019 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-31717325

RESUMO

The aim of this study was to establish the kinetics regression models for yield and composition of Ruta graveolens fruit and Hyssopus officinalis subsp. aristatus aboveground biomass essential oil (EO), collected at different time intervals during the hydrodistillation process. The hypothesis was that collecting the EO fractions during specific time frames may result in EOs with dissimilar composition that may have differential use by the industry. Furthermore, we calculated the kinetics regression models for the composition of EO, isolated by hydrodistillation in a Clevenger-type apparatus and characterized by GC-MS and GC-FID analyses. The EO yield of R. graveolens fruits was 0.39% (relative area % of GC-FID chromatogram), with major constituents in the Control fraction (0-90 min) being 2-nonanone, 2-undecanone, and 2-undecanol, representing 65% of the total oil. The highest concentration of 2-nonanone (60%) was found in the 30-60 min oil fraction, the concentration of 2-undecanone (35%) was highest in the Control (0-90 min) fraction, and the concentration of eucalyptol (19%) was highest in the 5-10 min fraction. The EO yield of H. officinalis subsp. aristatus dried biomass was 1.12%. The major constituents in the Control fraction (0-90 min) of H. officinalis biomass were eucalyptol, α-pinene, sabinene, ß-pinene, and cis-3-pinanone, representing 86% of the total. Eucalyptol (58%) was the highest in the 0-5 min fraction. The highest ß-pinene (15%) and cis-3-pinanone (20%) contents were found in the 20-40 min fraction. The kinetics regression models that were developed for EO composition of R. graveolens were second-order polynominal, Michaelis-Menten, and Exponential decay, while for EO composition of H. officinalis subsp. aristatus biomass were Exponential decay and Power. The results from this study could benefit the EO industry.


Assuntos
Biomassa , Destilação , Frutas/química , Hyssopus/química , Óleos Voláteis/química , Ruta/química , Cinética , Óleos Voláteis/análise , Compostos Fitoquímicos/análise , Compostos Fitoquímicos/química , Fatores de Tempo
10.
J Biosci ; 44(2)2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31180043

RESUMO

In this study, zinc oxide nanoparticles (ZnO-NPs) were synthesized using the extract of Hyssops officinalis L. via green method and confirmed by transmission electron microscopy, field emission scanning electron microscopy, X-ray powder diffraction and Fourier transforms infrared spectroscopy techniques. In the in vivo section, the anti-angiogenesis and antiinflammatory properties of the NPs were evaluated by the chorioallantoic membrane (CAM) assay and mouse paw edema test (induced by carrageenan), respectively. In the in vitro section, changes in the expression of angiogenesis genes (VEGF and VEGFR) and inflammatory genes (IL-1B and IL-10) were investigated by real-time quantitative polymerase chain reaction technique. In order to evaluate the cytotoxicity of ZnO-NPs, 3-5, 4-dimethylthiazol-2-yl) -5, 2-tetrazolium bromide (MTT) test was used on MDA-MB231 breast adenocarcinoma cell line. The results of the CAM assay showed that the ZnO-NPs significantly reduced the number and length of blood vessels, as well as the size and weight of the embryos. Evaluation of mouse paw edema showed that the NPs are able to decrease inflammation. Changes in the expression pattern of VEGF and VEGFR genes in MCF7 cells showed that the NPs have inhibitory effect on the expression of both genes. Expression levels of IL-10 and IL-1B genes also increased and decreased, respectively. The MTT test showed that the NP have the ability to decrease breast cancer cells. In conclusion, our results confirm that the ZnO-NPs synthesized by green method have promising anti-cancer properties.


Assuntos
Inibidores da Angiogênese/síntese química , Anti-Inflamatórios/síntese química , Citotoxinas/síntese química , Edema/tratamento farmacológico , Hyssopus/química , Nanopartículas/administração & dosagem , Óxido de Zinco/farmacologia , Inibidores da Angiogênese/farmacologia , Animais , Anti-Inflamatórios/farmacologia , Carragenina/administração & dosagem , Sobrevivência Celular/efeitos dos fármacos , Embrião de Galinha , Membrana Corioalantoide/irrigação sanguínea , Membrana Corioalantoide/efeitos dos fármacos , Citotoxinas/farmacologia , Edema/induzido quimicamente , Edema/fisiopatologia , Regulação da Expressão Gênica/efeitos dos fármacos , Química Verde , Membro Posterior , Humanos , Interleucina-10/genética , Interleucina-10/metabolismo , Interleucina-1beta/genética , Interleucina-1beta/metabolismo , Células MCF-7 , Camundongos , Nanopartículas/química , Extratos Vegetais/química , Fator A de Crescimento do Endotélio Vascular/genética , Fator A de Crescimento do Endotélio Vascular/metabolismo , Receptor 1 de Fatores de Crescimento do Endotélio Vascular/genética , Receptor 1 de Fatores de Crescimento do Endotélio Vascular/metabolismo , Óxido de Zinco/química
11.
Arkh Patol ; 81(1): 24-30, 2019.
Artigo em Russo | MEDLINE | ID: mdl-30830101

RESUMO

OBJECTIVE: To establish the mechanisms of pathomorphism of transplantable kidney cancer in rats that used flavonoid-containing hedge hyssop (Gratiola officinalis L.) extract in an in vivo experiments. MATERIAL AND METHODS: The experiment was carried out on 30 male Wistar rats with transplantable kidney cancer PA. At 72 hours after tumor inoculation, the rats in the experimental groups received hedge hyssop extract at an oral or intramuscular dose of 110 mg/kg/day for 12 days. A comparison group consisted of the animals with a tumor, but without exposure. The investigators used the immunohistochemical markers of apoptosis (p53, bax, bcl-2, Fas-receptor, Fas-ligand), autophagy (LC3B), proliferation (Ki-67), and angiogenesis (VEGF). During statistical data processing, the Shapiro-Wilk test was used to test the normality of the indicator distribution in the groups. Cramer-Welch's t-test was also employed to compare the groups. RESULTS: Histological examination of tumor tissue under the action of hedge hyssop extract showed the emergence of extensive areas of damage (necrosis and apoptotic bodies). With both routes of hedge hyssop extract administration, there was a sharp decrease in the expression of the proliferation markers Ki-67 and the angiogenesis marker VEGF and a high expression of the apoptosis markers p53, bax, CD95 (Fas/APO-1), and FAS-ligand in tumor cells and its absence in the comparison group. All the described changes were more pronounced with intramuscular administration. The expression of the autophagy marker LC3B increased with the oral administration of hedge hyssop extract and decreased with its intramuscular administration. CONCLUSION: A pronounced pathomorphism of kidney cancer develops due to consumption of hedge hyssop extract. This suppresses the proliferation, angiogenesis, and activation of apoptotic signaling and mitochondrial pathways and blocks protective autophagy. The autophagy marker LC3B can be used as an additional criterion for evaluating the therapeutic pathomorphism of tumors.


Assuntos
Apoptose , Autofagia , Flavonoides , Neoplasias Renais , Animais , Apoptose/efeitos dos fármacos , Apoptose/genética , Flavonoides/farmacologia , Hyssopus/química , Neoplasias Renais/metabolismo , Masculino , Extratos Vegetais/farmacologia , Proteínas Proto-Oncogênicas c-bcl-2 , Ratos , Ratos Wistar , Receptor fas
12.
Phytomedicine ; 57: 305-314, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30807985

RESUMO

BACKGROUND: Hyssopus cuspidatus Boriss has been used to treat bronchial asthma for many years in Uighur medicine. JAX2, an ethanol extract from this plant, has effectiveness against bronchial asthma. However, the molecular basis for the anti-inflammatory effects of JAX2 remains unclear. PURPOSE: This study aimed to evaluate the mechanism of JAX2 against bronchial asthma. METHODS: We established an asthma model in rats using ovalbumin (OVA), and an inflammatory model in RAW264.7 cells using lipopolysaccharide (LPS) stimulation. To evaluate the anti-inflammatory effects of JAX2, the concentrations of tumor necrosis factor (TNF)-α, interleukin (IL)-4, IL-6, IL-17, eotaxin and immunoglobulin (Ig)E were measured by enzyme-linked immunosorbent assay (ELISA). Cell viability was investigated by the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)2H-tetrazolium, inner salt (MTS) assay. Further, nitric oxide (NO) and reactive oxygen species (ROS) were determined using Griess reagent and 2,7-dichlorofluorescein diacetate. The phosphorylation of p-extracellular signal-regulated kinase (ERK), p-Jun-NH2-terminal kinase (JNK), p38 kinases (p38) and p-inhibitor of nuclear factor kappa-B kinase (IKK), and nuclear translocation of p-p65 kinases (p-p65) were determined by immunofluorescence to uncover the effects of JAX2 on the Mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B (NF-κB) signaling pathways. RESULTS: After JAX2 administration to rats, Interferon (IFN)-γ concentrations in BALF increased significantly. Further, the concentrations of TNF-α, IL-4, IL-6, IL-17 and eotaxin in BALF, and IgE in serum decreased. JAX2 decreased TNF-α, IL-6 and NO in cell supernatant, and reduced ROS intracellularly. Concurrently, IFN-γ concentrations increased in cell supernatant significantly. In LPS-induced RAW264.7 cells, JAX2 inhibited phosphorylation of p-ERK, p-JNK and p-38 MAPK. The subsequent phosphorylation of p-IKK and nuclear translocation of the p-p65 subunit of NF-κB were also suppressed. CONCLUSION: Based on these findings, we believe that JAX2 has both preventive and treatment effects in bronchial asthma. Furthermore, in the RAW264.7 cell inflammatory model, JAX2 also inhibited NF-κB and MAPK signaling pathways.


Assuntos
Antiasmáticos/farmacologia , Asma/prevenção & controle , Hyssopus/química , Extratos Vegetais/farmacologia , Animais , Asma/metabolismo , Modelos Animais de Doenças , Etanol/química , Feminino , Lipopolissacarídeos/farmacologia , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Masculino , Camundongos , NF-kappa B/metabolismo , Óxido Nítrico/metabolismo , Fosforilação/efeitos dos fármacos , Extratos Vegetais/química , Células RAW 264.7 , Ratos Sprague-Dawley , Transdução de Sinais/efeitos dos fármacos
13.
Nat Prod Res ; 33(6): 866-870, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29179564

RESUMO

Hyssopus seravshanicus (Dubj.) Pazij has been used as traditional herb and food due to its wide biological properties. Seventeen known compounds were isolated from the ethyl acetate fraction. Their structures were identified by spectroscopic data and comparison with published data. Among them, 14 ones were identified from H. seravshanicus for the first time. DPPH and ABTS radical scavenging activities for crude ethanol extract (CEE), ethyl acetate fraction (EAF), butanol fraction (BF) and compounds 1, 3, 8, 10, 11 and 13 were performed. CEE, compounds 1, 3, 11 and 13 exhibited potent antioxidant activities. Compound 1 was found to increase the melanin content and tyrosinase activity of B16 melanoma cells. Moreover, the quantitative estimation of compound 1 in the ethyl acetate fraction was carried out by UPLC-DAD and the method was validated. This is the first report on the isolation and bioactivity research on the non-valotile components of H. seravshanicus.


Assuntos
Antioxidantes/farmacologia , Hyssopus/química , Melaninas/biossíntese , Monofenol Mono-Oxigenase/metabolismo , Extratos Vegetais/farmacologia , Animais , Antioxidantes/isolamento & purificação , Melanoma Experimental , Camundongos , Estrutura Molecular , Oxirredução , Compostos Fitoquímicos/isolamento & purificação , Compostos Fitoquímicos/farmacologia
14.
J Chromatogr A ; 1579: 9-19, 2018 Dec 07.
Artigo em Inglês | MEDLINE | ID: mdl-30430990

RESUMO

To determine the optimum condition for the extraction of Hyssopus officinalis L. essential oil (EO) by instant controlled pressure drop (in French: Détente Instantanée Contrôlée or DIC), the influential parameters of pressure, heating time per cycle, number of cycles, mean particle diameter of the herb, and water concentration in the steam generator feed were evaluated using response surface methodology (RSM). The impact of using an ethanol-water mixture to generate the required vapor in the DIC process on EO extraction was investigated. The optimum condition was found to be at pressure = 1 bar, heating time = 100 s, cycles = 12, particle diameter = 1 mm, and water concentration = 80 v%. The addition of ethanol turned out to be more efficient at higher heating times and cycle numbers. The optimum yield in DIC was highest compared with hydrodistillation (HD), ultrasound-assisted extraction (UAE), and Soxhlet (SOX) methods; their values, expressed as total area percentages per internal standard area percentage, were 39.04, 10.98, 1.58, and 7.71, respectively. Comparison of the four methods also indicated that the DIC-EO was of a superior quality. The analysis of the DIC-treated residual herb in the optimum experiment revealed that unlike the low amount of valuable oxygenated monoterpenes, the availability of sesquiterpenes increased, which proved that DIC performs selectively while extracting more valuable EO components. The DIC-EO and its extract were examined for total phenolic content (TPC) and antioxidant activity using Folin-Ciocalteu and DPPH assay, respectively. Comparison of these results with those obtained from HD demonstrated higher TPC and antioxidant activity of the DIC-EO. Moreover, scanning electron microscopy (SEM) of the raw material and residual herb in the optimum experiment showed significant structure modifications induced by the DIC process.


Assuntos
Técnicas de Química Analítica/métodos , Hyssopus/química , Óleos Voláteis/isolamento & purificação , Pressão , Etanol/química , Temperatura Alta , Fenóis/análise , Sesquiterpenos/química , Vapor
15.
Chem Biodivers ; 15(1)2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-28801935

RESUMO

This work presents the biocidal (insecticidal, ixodicidal, nematicidal, and phytotoxic) effects and chemical compositions of three essential oils obtained from the industrial steam distillation (IEOs) of hyssop (Hyssopus officinalis L.), lavandin (Lavandula × intermedia or L. × hybrida var. Super), and cotton lavender (Santolina chamaecyparissus L.). Their chemical composition analyzed by gas chromatography coupled to mass spectrometry showed 1,8-cineole (53%) and ß-pinene (16%) as the major components of H. officinalis, linalyl acetate (38%) and linalool (29%) of L. × intermedia; and 1,8-cineole (10%) and 8-methylene-3-oxatricyclo[5.2.0.02,4 ]nonane (8%) in S. chamaecyparissus. The biocidal tests showed that L. × intermedia IEO was the most active against the insect Spodoptera littoralis and toxic to the tick Hyalomma lusitanicum, IEO of H. officinalis was strongly active against S. littoralis, and finally, S. chamaecyparissus IEO was a strong antifeedant against the aphid Rhopalosiphum padi, toxic to H. lusitanicum and with moderate effects against Leptinotarsa decemlineata, S. littoralis, and Lolium perenne.


Assuntos
Asteraceae/química , Hyssopus/química , Inseticidas/farmacologia , Lavandula/química , Óleos Voláteis/farmacologia , Animais , Afídeos/efeitos dos fármacos , Relação Dose-Resposta a Droga , Inseticidas/química , Inseticidas/isolamento & purificação , Óleos Voláteis/química , Óleos Voláteis/isolamento & purificação , Spodoptera/efeitos dos fármacos , Relação Estrutura-Atividade , Carrapatos/efeitos dos fármacos
16.
Parasitol Int ; 66(2): 166-171, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-28110082

RESUMO

Mosquito vector control is facing a number of important and timely challenges, mainly due to the rapid development of pesticide resistance and environmental concerns. In this scenario, screening of botanical resources for their mosquitocidal activity may offer effective and eco-friendly tools against Culicidae vectors. Culex quinquefasciatus Say (Diptera: Culicidae) is a vector of lymphatic filariasis and of dangerous arboviral diseases, such as West Nile and St. Louis encephalitis. In this study, the chemical composition of five essential oils obtained from different plants, namely Pinus nigra J.F. Arnold var. italica (Pinaceae), Hyssopus officinalis L. subsp. aristatus (Lamiaceae), Satureja montana L. subsp. montana (Lamiaceae), Aloysia citriodora Palau (Verbenaceae) and Pelargonium graveolens L'Hér (Geraniaceae), was investigated by GC-MS analysis. Furthermore, it was evaluated their acute toxicity on larvae of C. quinquefasciatus. Then, the most effective oils were selected, in order to focus on the potential synergistic and antagonistic effects, testing them in binary mixtures on C. quinquefasciatus larvae. Results showed that the higher effectiveness was obtained by S. montana subsp. montana essential oil (LC50=25.6µL·L-1), followed by P. nigra var. italica (LC50=49.8µL·L-1) and A. citriodora (LC50=65.6µL·L-1), while the other essential oils showed LC50 values higher than 90µL·L-1. The larvicidal effectiveness can be enhanced by preparing simple binary mixtures of essential oils, such as S. montana+A. citriodora (ratio 1:1), which showed higher larvicidal toxicity (LC50=18.3µL·L-1). On the other hand, testing S. montana+P. nigra (1:1) an antagonistic effect was detected, leading to a LC50 (72.5µL·L-1) higher than the LC50 values calculated for the two oils tested separately. Overall, our results add useful knowledge to allow the employ of synergistic essential oil blends as effective, cheap and eco-friendly mosquito larvicides.


Assuntos
Culex/efeitos dos fármacos , Inseticidas/farmacologia , Mosquitos Vetores/efeitos dos fármacos , Óleos Voláteis/farmacologia , Óleos Vegetais/farmacologia , Animais , Antagonismo de Drogas , Sinergismo Farmacológico , Filariose Linfática/prevenção & controle , Filariose Linfática/transmissão , Encefalite de St. Louis/prevenção & controle , Encefalite de St. Louis/transmissão , Cromatografia Gasosa-Espectrometria de Massas , Hyssopus/química , Inseticidas/química , Larva/efeitos dos fármacos , Óleos Voláteis/química , Pelargonium/química , Pinus/química , Folhas de Planta/química , Óleos Vegetais/química , Satureja/química , Verbenaceae/química , Febre do Nilo Ocidental/prevenção & controle , Febre do Nilo Ocidental/transmissão
17.
Acta Biol Hung ; 67(2): 195-204, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-27165530

RESUMO

The relationships between salt stress and antioxidant enzymes activities, proline, phenol and anthocyanine contents in Hyssopus officinalis L. plants in growth stage were investigated. The plants were subjected to five levels of saline irrigation water, 0.37 (tap water as control) with 2, 4, 6, 8 and 10 dSm(-1) of saline water. After two months the uniform plants were harvested for experimental analysis. Antioxidant enzymes activities and proline, phenol and anthocyanine contents of the plants were examinated. Enhanced activities of peroxidase, catalase and superoxide dismutase were determined by increasing salinity that plays an important protective role in the ROS-scavenging process. Proline, phenol and anthocyanine contents increased significantly with increasing salinity. These results suggest that salinity tolerance of Hyssopus officinalis plants might be closely related with the increased capacity of antioxidative system to scavenge reactive oxygen species and with the accumulation of osmoprotectant proline, phenol and anthocyanine contents under salinity conditions.


Assuntos
Antioxidantes/metabolismo , Hyssopus/enzimologia , Salinidade , Antocianinas/metabolismo , Fenóis/metabolismo , Prolina/metabolismo , Estresse Fisiológico
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