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1.
Electron. j. biotechnol ; 12(3): 12-13, July 2009. ilus, tab
Artigo em Inglês | LILACS | ID: lil-551890

RESUMO

16S ribosomal RNA (rRNA)-targeted fluorescent in situ hybridization combined with polymerase chain reaction (PCR)-cloning, light microscopy using Gram stains, scanning electron microscopy and denatured gradient gel electrophoresis were used to reveal the distribution of methanogens within an anaerobic closed digester tank fed with palm oil mill effluent. For specific detection of methanogens, 16S rRNA-cloning analysis was conducted followed by restriction fragment length polymorphism (RFLP) for presumptive identification of methanogens. To cover the drawbacks of the PCR-cloning study, the organization of the microorganisms was visualized in the activated sludge sample by using fluorescent oligonucleotide probes specific to several different methanogens, and a probe for bacteria. In situ hybridization with methanogens and bacterial probes and denatured gradient gel electrophoresis within activated sludge clearly confirmed the presence of Methanosaeta sp. and Methanosarcina sp. cells. Methanosaeta concilii was found to be the dominant species in the bioreactor. These results revealed the presence of possibly new strain of Methanosaeta in the bioreactor for treating palm oil mill effluent called Methanosaeta concilii SamaliEB (Gene bank accession number: EU580025). In addition, fluorescent hybridization pictured the close association between the methanogens and bacteria and that the number of methanogens was greater than the number of bacteria.


Assuntos
Óleo de Palmeira/análise , Clonagem Molecular , Digestão Anaeróbia/análise , Genes de RNAr , Methanosarcina/isolamento & purificação , Methanosarcinales/isolamento & purificação , Óleo de Palmeira , Tanques Imhoff/análise , Hibridização in Situ Fluorescente , Reação em Cadeia da Polimerase/métodos
2.
In. AIDIS. Trabajos presentados: tomo 1. Buenos Aires, AIDIS, 1999. p.1-19.
Monografia em Espanhol, Espanhol | BINACIS | ID: bin-139381

RESUMO

Describe las caracteristicas generales de el pozo IMHOFF, sus criterios de aplicacion y criterios de dimensionado. Se describen ademas las caracteristicas de los filtros biologicos, sus criterios de aplicacion, los parametros de entrada para la etapa del tratamiento biologico y los del proceso


Assuntos
Tanques Imhoff , Digestores , Filtros Biológicos , Digestão Anaeróbia
3.
In. Anon. Memoria del curso: Microbiología y aplicaciones en los procesos biológicos de tratamiento de aguas. México, SIE, 1983. p.1-14, ilus.
Monografia em Espanhol | LILACS | ID: lil-146339

RESUMO

Describe el tanque Imhoff el cual es recomendado para aguas residuales domesticas y para comunidades de 50000 personas. Presenta una remoción de 40 al 65 por ciento dependiendo de la concentración de sólidos sedimentables en mg/l y del tiempo de retención. Presenta las bases y las formulas para su diseño y algunos ejemplos de aplicación. Incluye tres métodos comunes para la disposición de lodos


Assuntos
Eliminação Final de Lodos , Saúde Ambiental , Tanques Imhoff
4.
Buenos Aires; AIDIS; s.n. (64835).
Monografia em Espanhol | BINACIS | ID: bin-64835

RESUMO

Describe las caracteristicas generales de el pozo IMHOFF, sus criterios de aplicacion y criterios de dimensionado. Se describen ademas las caracteristicas de los filtros biologicos, sus criterios de aplicacion, los parametros de entrada para la etapa del tratamiento biologico y los del proceso


Assuntos
Tanques Imhoff , Digestores , Filtros Biológicos , Digestão Anaeróbia
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