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1.
Food Chem ; 371: 131366, 2022 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-34808770

RESUMO

In this paper, NC QDs were obtained from agricultural waste (wheat straw), functionalized by 4,5-imidazole dicarboxylic acid and used to detect gentamicin in aqueous solution. The NC QDs were characterized by HRTEM, XPS, UV-Vis, etc. The fluorescence properties of the functionalized NC QDs for GEN detection were studied at λem = 385 nm. The fluorescence intensity showed a good linear relationship with the GEN concentration of samples (I = 343.10 + 30.07C, R2 = 0.9896) for GEN concentrations ranging from 0 to 2.9 × 10-4 mol/L. The sensing mechanism was found to be fluorescence resonance energy transfer (FRET) between the functionalized NC QDs and GEN. The results indicated that the method had good repeatability for GEN detection. The technology could be extended to biological detection.


Assuntos
Pontos Quânticos , Carbono , Transferência Ressonante de Energia de Fluorescência , Corantes Fluorescentes , Gentamicinas , Imidazóis , Nitrogênio
2.
J Pharm Biomed Anal ; 207: 114395, 2022 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-34628292

RESUMO

For the robust analysis of N,N-Carbonyldiimidazole (CDI), its derivatization into a more stable compound may be needed. Herein, the reaction of CDI with N-benzylmethylamine followed by LC-UV quantitative analysis was explored. Reaction conditions as well as LC method feasibility were demonstrated by qualification of selectivity from other impurities and reagents, linearity across a range of 0.05-0.15%w/w, spike and recovery across a range of 0.05-0.15%w/w, reaction reproducibility with various samples, reagents and analytical chemists, and sample stability of over 24 h. Rapid and quantitative derivatization of residual CDI was achieved at 0.1% w/w relative to the synthetic product under consideration. A fit-for-purpose limit test using a RPLC-UV method as an in-process control for the reaction completion of product, at scale, was successfully implemented and executed.


Assuntos
Imidazóis , Indicadores e Reagentes , Reprodutibilidade dos Testes
3.
Spectrochim Acta A Mol Biomol Spectrosc ; 264: 120235, 2022 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-34371314

RESUMO

Curcumin is a natural product that is frequently utilized in cancer prevention and treatment. The significant benefit of vegetable-derived nutraceuticals in combination with widespread cytostatic medication such as ponatinib is to reduce toxicity and side effects. In this paper, we focus the study on analytical quantification of ponatinib and curcumin through highly sensitive synchronous spectrofluorometric method. Applying this method at Δλ = 160 nm, each of ponatinib and curcumin could be measured at 303 and 412 nm without interference from each others. The diverse experimental factors impacting the performance of the method were studied and optimized. The method exhibited a reasonable linearity in the ranges of 5.0-60.0 and 10.0-200.0 ng/mL for ponatinib and curcumin, respectively with detection limits of 1.48 and 1.22 ng/mL and quantitation limits of 4.49 and 3.68 ng/mL, respectively. The anticipated method was employed for the assessment and evaluation of the studied drugs in the spiked human plasma samples. The mean % recoveries in plasma samples (n = 6) for each of ponatinib and curcumin were 99.84 ± 1.86 and 100.06 ± 2.72, accordingly. The developed method was validated in conformity with the requirements of International Council of Harmonization (ICH).


Assuntos
Curcumina , Humanos , Imidazóis , Laboratórios , Piridazinas , Espectrometria de Fluorescência
4.
Chemosphere ; 286(Pt 1): 131578, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34303052

RESUMO

Ionic liquids (ILs) are used as detoxication agents for fermentation of lignin into ethanol because of their good applicability. However, the residual ILs may be toxic to the yeast. In order to improve the use of ILs for fermentation and protected environment, the toxicity of ILs with different carbon chain length to Pichia stipitis was studied in this paper. Four kinds of common imidazolium chloride ILs ([C4mim]Cl, [C6mim]Cl, [C8mim]Cl and [C10mim]Cl) were selected. ILs can inhibit the proliferation of Pichia stipitis and increase their mortality. Oxidative stress reaction occurred in the cells, and the activities of antioxidant enzymes are affected. Comparing with the integrated biomarker response (IBR) index, it was found that the toxicity increases with increasing chain length. ILs may enter cells by damaging cell membranes and reduce ethanol production by damaging organelles such as mitochondria. ILs caused wrinkles and dents on the surface of cells up to cell deformation and even rupture. The toxicity sequence was as follows: [C10mim]Cl> [C8mim]Cl>[C6mim]Cl>[C4mim]Cl. Due to this toxicity to Pichia stipitis, these compounds should be used carefully in the fermentation process and also to avoid toxic effects on other organisms in the environment.


Assuntos
Líquidos Iônicos , Carbono , Imidazóis/toxicidade , Líquidos Iônicos/toxicidade , Saccharomycetales
5.
Talanta ; 237: 122925, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34736662

RESUMO

Herein, a facile and fast surface-enhanced Raman scattering (SERS) method with ratiometric strategy was developed for detection of 4-methylimidazole (4-MI). Via a chemical derivatization reaction with 3-amino-5-mercapto-1,2,4-triazole (AMTA) diazonium salts, 4-MI could be converted to SERS-sensitive species. The SERS intensity ratio between the peaks at 1243 cm-1 and 1110 cm-1 (I1243/I1110) was used for the quantification of 4-MI. In addition, the method sensitivity was further improved by the aggregation of beta-cyclodextrin-modified Ag nanoparticles (beta-CD-AgNPs). Under the optimal conditions, the limit of detection (LOD) and the limit of quantification (LOQ) for 4-MI were 1.7 nM (S/N = 3) and 5.7 nM (S/N = 10), respectively. The relative standard deviation (RSD) for 0.5 µM 4-MI was 8.2% (n = 20). This method was successfully used for the determination of 4-MI in cola samples with recoveries ranging from 92% to 106%. The present method is convenient, sensitive, selective, reliable and may have a promising application in determination of the compounds with an imidazole ring containing active hydrogen atoms.


Assuntos
Nanopartículas Metálicas , Análise Espectral Raman , Imidazóis , Limite de Detecção , Prata
6.
Food Chem ; 369: 130952, 2022 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-34474283

RESUMO

l-glycine and l-serine are the building blocks of proteins and exhibit various biological activities. This work found that l-glycine and l-serine show low scavenging capacity for methylglyoxal at moderate conditions (pH 7.0, 37 °C). However, they efficiently eliminate methylglyoxal and formaldehyde when the two aldehydes co-exist, via generation of imidazole salt, a compound formed by one molecule of methylglyoxal and formaldehyde, and two molecules of amino acids. The imidazole salts were identified in biscuits and fried potato crisps. Moreover, the formation of imidazole salts greatly decreased the cytotoxicity of their precursors, methylglyoxal and formaldehydes. This finding suggests that glycine and serine can be used to scavenge these two harmful aldehydes both after intake and during food processing.


Assuntos
Glicina , Aldeído Pirúvico , Formaldeído , Imidazóis , Sais , Serina
7.
Water Sci Technol ; 84(9): 2288-2303, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34810312

RESUMO

In recent research, the composite of Fe3O4 and metal-organic frameworks have shown great potential in removing potentially toxic metals from water. We conducted the adsorption studies of potentially toxic metal ions (Cu2+, Co2+ and Cd2+) using the composite of Fe3O4 and zeolitic imidazole framework-8 (Fe3O4@ZIF-8) for the first time. The solvothermal technique was used to synthesize the Fe3O4. The magnetic ZIF-8 offers high thermal stability, greater adsorption surface, good removability, and high chemical and thermal stability. Characterization techniques such as X-ray diffraction (XRD), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), X-ray photoelectron spectroscopy (XPS) and Fourier-transform infrared spectroscopy (FTIR) were used to characterize the synthesized samples. The SEM and XRD results revealed the high purity and structural integrity of ZIF-8 crystallites. To remove potentially toxic metals (Cu2+, Co2+ and Cd2+), the influence of adsorbent dosage, contact time, pH, and adsorbate concentration on the adsorption performance of Fe3O4@ZIF-8 was investigated. The Langmuir isotherm accurately represented the adsorption processes, with absorption magnitudes of Fe3O4@ZIF-8 determined to be 46.82 mg g-1, 71.29 mg g-1 and 54.49 mg g-1 for Cu2+, Co2+ and Cd2+, respectively. According to the adsorption mechanism analysis, the primary Cu2+, Co2+ and Cd2+ removal methods of Fe3O4@ZIF-8 were ion exchange and coordination bonds. The uptake capacity of Cu2+, Co2+ and Cd2+ solution by Fe3O4@ZIF-8 were not significantly affected by the presence of counter ions. The material exhibited superior regenerative properties for Cu2+, Co2+ and Cd2+ ions from water for up to three cycles. This study concluded that the Fe3O4@ZIF-8 could be a viable candidate for eliminating potentially toxic metals (Cu2+, Co2+ and Cd2+).


Assuntos
Poluentes Químicos da Água , Zeolitas , Adsorção , Cádmio , Cobalto , Concentração de Íons de Hidrogênio , Imidazóis , Íons , Ferro , Cinética , Óxidos , Água , Poluentes Químicos da Água/análise
9.
Environ Sci Technol ; 55(22): 15301-15312, 2021 11 16.
Artigo em Inglês | MEDLINE | ID: mdl-34719228

RESUMO

Zeolitic imidazolate frameworks (ZIFs) have been developed quickly and have attracted considerable attention for use in the detection and removal of various pollutants. Understanding the environmental risks of ZIFs is a prerequisite to their safe application by industry and new chemical registration by governments; however, the persistence and recovery of toxicity induced by ZIFs remain largely unclear. This study finds that typical ZIFs (e.g., ZIF-8 and ZIF-67) at a concentration of 0.01-1 mg/L induce significant algal growth inhibition, plasmolysis, membrane permeability, chloroplast damage, and chlorophyll biosynthesis, and the above alterations are recoverable. Unexpectedly, a persistent decrease in reactive oxygen species (ROS) is observed due to the quenching of hydroxyl free radicals. The adverse effects of ZIF-8 are weak and easily alleviated compared with those of ZIF-67. ZIF-8 is internalized mainly by caveolae-mediated endocytosis, while ZIF-67 is internalized mainly by clathrin-mediated endocytosis. Omics studies reveal that the downregulation of mRNA associated with oxidative phosphorylation and the inhibition of chlorophyll and adenosine triphosphate (ATP) synthesis in mitochondria are related to the persistence of phytotoxicity. These findings highlight the phenomena and mechanisms of the persistence and recovery of phytotoxicity, indicating the need to reconsider the environmental risk assessments of ZIFs.


Assuntos
Imidazóis , Zeolitas , Atenção , Imidazóis/toxicidade , Zeolitas/toxicidade
10.
J Agric Food Chem ; 69(46): 13933-13941, 2021 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-34756022

RESUMO

The effects of the deacetylation degree (DD) of chitosan on heterocyclic aromatic amine formation were investigated in chemical models and beef patties. The results in model systems showed that at lower addition levels (10 mg), chitosan with 85% DD showed the strongest inhibitory effect against 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) formation, while chitosan with a higher DD (95%) or a lower DD (72 and 50%) did not show any significantly inhibitory effect. Further mechanism study showed that chitosan addition reduced the content of Maillard reaction intermediates including phenylacetaldehyde and the aldol condensation product but increased the PhIP precursor creatinine residue in the chemical model, indicating that chitosan at least partially competed with creatinine to react with phenylacetaldehyde to inhibit PhIP formation. In roast beef patties, 0.15% (w/w) chitosan (85% DD) significantly reduced the formation of PhIP, MeIQx, 4,8-DiMeIQx, Harman, and Norharman by 56.21, 33.32, 31.35, 25.14, and 28.12%, respectively. Moreover, chitosan significantly inhibited the formation of aldehydes in roast beef patties, further confirming the above-mentioned inhibition mechanism. However, the addition of chitosan might promote fatty acid oxidation. In addition, chitosan addition below 0.15% (w/w) had no significant effect on the textural properties of the roast samples.


Assuntos
Quitosana , Modelos Químicos , Animais , Bovinos , Culinária , Imidazóis , Carne/análise , Piridinas
11.
J Agric Food Chem ; 69(46): 13871-13880, 2021 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-34780187

RESUMO

IGPD is an essential metalloenzyme that catalyzes histidine biosynthesis. We found that its C-terminus loop region has a vital role in determining enzyme activity but has been hardly mentioned before. In this work, we focused on the dynamic feature and function of C-Loop in Arabidopsis thaliana and Saccharomyces cerevisiae IGPD (At_IGPD and Sc_IGPD, respectively). Due to the high flexibility of this region, we performed a total of 3.4 µs of accelerated molecular dynamics simulation to enhance sampling. Inhibitor C348 in At-IGPD exhibited instability in the later stage of simulation, while the characteristic sequence in Sc_IGPD reduced solvent interference and significantly restrained the interaction mode. For the C-Loop-assisted ligand-binding process, we proposed a "Lock-Lid" model. Meanwhile, the dissociated ligand in At_IGPD served as a probe, a metastable pocket was determined at the root of C-Loop, and its rationality was proved by theoretical verification and enzyme mutation experiments. This study complemented the important structural features of C-Loop and provided a basis for the design of selective inhibitors. Considering the absence in mammals, we suggested that IGPD could be a promising germicide target.


Assuntos
Glicerol , Fosfatos , Animais , Hidroliases/genética , Imidazóis , Saccharomyces cerevisiae/genética
12.
Korean J Parasitol ; 59(5): 481-487, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-34724767

RESUMO

The objective of this study was to evaluate the efficacy of an imidacloprid 10% and flumethrin 4.5% polymer matrix collar against the developmental stages of Haemaphysalis longicornis infesting dogs using the hair from treated dogs in a semi-in-vitro assay set. When incubated with 0.5 g of the hair collected from the dogs installed with the drug-embedded collar after 10 days, average death rate of the larval, nymphal, and adult H. longicornis was 21.5%, 77.9%, and 100% at 30 min, 1 hr, and 2 hr, respectively. This study showed the larval stages as well as the nymphal and adult stages of H. longicornis ticks are killed upon contact with the hair from dogs treated with the collar within 2 hr.


Assuntos
Ixodidae , Infestações por Carrapato , Animais , Cães , Imidazóis , Larva , Neonicotinoides , Nitrocompostos , Polímeros , Piretrinas , Infestações por Carrapato/veterinária
13.
Molecules ; 26(19)2021 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-34641279

RESUMO

(1) Background: different previously synthesized pyrazoles and imidazo-pyrazoles showed interesting anti-angiogenic action, being able to interfere with ERK1/2, AKT and p38MAPK phosphorylation in different manners and with different potency; (2) Methods: here, a new small compound library, endowed with the same differently decorated chemical scaffolds, has been synthetized to obtain new agents able to inhibit different pathways involved in inflammation, cancer and human platelet aggregation. (3) Results: most of the new synthesized derivatives resulted able to block ROS production, platelet aggregation and p38MAPK phosphorylation both in platelets and Human Umbilical Vein Endothelial cells (HUVEC). This paves the way for the development of new agents with anti-angiogenic activity.


Assuntos
Anti-Inflamatórios/síntese química , Imidazóis/química , Pirazóis/química , Bibliotecas de Moléculas Pequenas/síntese química , Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo , Anti-Inflamatórios/química , Anti-Inflamatórios/farmacologia , Células Endoteliais da Veia Umbilical Humana , Humanos , Fosforilação/efeitos dos fármacos , Agregação Plaquetária/efeitos dos fármacos , Espécies Reativas de Oxigênio/metabolismo , Bibliotecas de Moléculas Pequenas/química , Bibliotecas de Moléculas Pequenas/farmacologia , Relação Estrutura-Atividade
14.
Value Health Reg Issues ; 26: 182-190, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34673349

RESUMO

OBJECTIVES: Advanced melanoma accounts for 4% of malignant skin tumors, and approximately 80% of deaths are attributed to it. The most frequent mutation of the RAF gene is BRAFV600, which has been associated with a worse prognosis. The objective of the research was to evaluate the cost-effectiveness of the combined regimen of dabrafenib plus trametinib (D + T) compared with other targeted therapies, immunotherapy, and dacarbazine for the treatment of unresectable/metastatic melanoma with BRAFV600 mutation from the perspective of the Colombian health system. METHODS: A partitioned survival model with 3 states (progression-free survival, progression, and death) was used to evaluate the cost-effectiveness for a time horizon of 20 years. Owing to the perspective of the analysis, only direct medical costs were taken into account. The efficacy of the evaluated treatment and the comparators were measured in terms of overall survival and progression-free survival. All costs were expressed in Colombian pesos as of 2018, and outcomes and costs were discounted at 5% annually. Two analysis scenarios were considered, one in which only monitoring and follow-up costs were included in the progression phase and another in which costs of acquisition of possible treatment sequences were also included. RESULTS: In the first scenario (without postprogression medication costs), the combined D + T regimen was a dominant alternative to vemurafenib + cobimetinib but was not a cost-effective option compared with vemurafenib, nivolumab, ipilimumab, nivolumab + ipilimumab, pembrolizumab, and dacarbazine. In the second scenario (with drug costs in postprogression), D + T was dominant compared with vemurafenib + cobimetinib and cost-effective compared with nivolumab and pembrolizumab. Compared with other schemes, the incremental cost-effectiveness ratio was above the threshold of 3 gross domestic product per capita. Probabilistic sensitivity analyses showed that a willingness-to-pay threshold of Col$56 484 300 (US$19 108) per quality-adjusted life-year would not be reached at the current price of schema in Colombia. CONCLUSIONS: The combined scheme could be a cost-effective and even a cost-saving alternative to vemurafenib + cobimetinib, nivolumab, and pembrolizumab if the costs associated with the use of other medications are taken into account after progression to the first line of treatment. Compared with the other comparators, it produces a greater number of quality-adjusted life-years, but the incremental cost-effectiveness ratio is above that of the willingness to pay.


Assuntos
Dacarbazina , Melanoma , Colômbia , Análise Custo-Benefício , Humanos , Imidazóis , Imunoterapia , Melanoma/tratamento farmacológico , Melanoma/genética , Mutação , Oximas , Piridonas , Pirimidinonas
15.
J Chromatogr A ; 1658: 462592, 2021 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-34656844

RESUMO

A new method involving matrix solid-phase dispersion (MSPD) and dispersive liquid-liquid microextraction (DLLME) was optimized with the aid of stoichiometry and applied to the extraction, purification, and determination of benzimidazole residues in vegetables. Carbendazim, thiabendazole, and thiophanate-methyl were selectively extracted from vegetables using cucurbit[7]uril as the MSPD extractant and transferred to an aqueous solution, then further enriched using DLLME with acetonitrile and chloroform as dispersive and extraction solvents, respectively. The optimal extraction conditions of MSPD and DLLME were selected by two-level full-factorial design and central-composite design (CCD). The developed method (MSPD-DLLME-HPLC-UV) showed good linearity in the range of 0.025-5 µg/g, with R2 > 0.9984. Intra- and interday precisions were 5.3-10.9% and 10.6-12.4%, respectively, and the limit of detection was between 0.004 and 0.007 µg/g of fresh weight. This method was applied to the analysis of four different types of vegetables, and the recoveries ranged from 65.4% to 124.0%. The method was environmentally friendly, easy to operate, and sensitive.


Assuntos
Fungicidas Industriais , Microextração em Fase Líquida , Benzimidazóis/análise , Hidrocarbonetos Aromáticos com Pontes , Cromatografia Líquida de Alta Pressão , Fungicidas Industriais/análise , Imidazóis , Verduras
16.
Prog Brain Res ; 266: 1-73, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34689857

RESUMO

Military personnel deployed in combat operations are highly prone to develop Parkinson's disease (PD) in later lives. PD largely involves dopaminergic pathways with hallmarks of increased alpha synuclein (ASNC), and phosphorylated tau (p-tau) in the cerebrospinal fluid (CSF) precipitating brain pathology. However, increased histaminergic nerve fibers in substantia nigra pars Compacta (SNpc), striatum (STr) and caudate putamen (CP) associated with upregulation of Histamine H3 receptors and downregulation of H4 receptors in human cases of PD is observed in postmortem cases. These findings indicate that modulation of histamine H3 and H4 receptors and/or histaminergic transmission may induce neuroprotection in PD induced brain pathology. In this review effects of a potent histaminergic H3 receptor inverse agonist BF-2549 or clobenpropit (CLBPT) partial histamine H4 agonist with H3 receptor antagonist, in association with monoclonal anti-histamine antibodies (AHmAb) in PD brain pathology is discussed based on our own observations. Our investigation shows that chronic administration of conventional or TiO2 nanowired BF 2649 (1mg/kg, i.p.) or CLBPT (1mg/kg, i.p.) once daily for 1 week together with nanowired delivery of HAmAb (25µL) significantly thwarted ASNC and p-tau levels in the SNpC and STr and reduced PD induced brain pathology. These observations are the first to show the involvement of histamine receptors in PD and opens new avenues for the development of novel drug strategies in clinical strategies for PD, not reported earlier.


Assuntos
Fármacos Neuroprotetores , Doença de Parkinson , Corpo Estriado , Histamina , Humanos , Imidazóis , Fármacos Neuroprotetores/farmacologia , Doença de Parkinson/tratamento farmacológico , Receptores Histamínicos H4 , Tioureia/análogos & derivados
17.
Curr Hematol Malig Rep ; 16(5): 448-454, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-34661874

RESUMO

PURPOSE OF REVIEW: Chronic myeloid leukemia (CML) is a myeloproliferative neoplasm characterized by uncontrolled proliferation of mature and maturing granulocytes. The disease is characterized by the presence of translocation t(9;22) leading to the abnormal BCR-ABL fusion. Historically, treatment options included hydroxyurea, busulfan, and interferon-α (IFN-α), with allogeneic stem cell transplant being the only potential curative therapy. More recently, the development of tyrosine kinase inhibitors (TKIs) has revolutionized the treatment of CML and turned a once fatal disease into a chronic and manageable disorder. This review aims to discuss the frontline treatment options in chronic-phase CML, provide recommendations for tailoring frontline treatment to the patient, and explore emerging therapies in the field. RECENT FINDINGS: The first-generation TKI, imatinib, was FDA approved in 2001 for use in CML. Following the approval and success of imatinib, second- and third-generation TKIs have been developed providing deeper responses, faster responses, and different toxicity profiles. With numerous options available in the frontline setting, choosing the best initial treatment for each individual patient has become a more complex decision. When choosing a frontline therapy for patients with chronic-phase CML, one should consider disease risk, comorbid conditions, and the goal of therapy.


Assuntos
Antineoplásicos/uso terapêutico , Leucemia Mieloide de Fase Crônica/tratamento farmacológico , Inibidores de Proteínas Quinases/uso terapêutico , Compostos de Anilina/uso terapêutico , Animais , Dasatinibe/uso terapêutico , Humanos , Mesilato de Imatinib/uso terapêutico , Imidazóis/uso terapêutico , Nitrilas/uso terapêutico , Proteínas Tirosina Quinases/antagonistas & inibidores , Piridazinas/uso terapêutico , Pirimidinas/uso terapêutico , Quinolinas/uso terapêutico
18.
PLoS Pathog ; 17(10): e1009928, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-34695164

RESUMO

Non-specific protective effects of certain vaccines have been reported, and long-term boosting of innate immunity, termed trained immunity, has been proposed as one of the mechanisms mediating these effects. Several epidemiological studies suggested cross-protection between influenza vaccination and COVID-19. In a large academic Dutch hospital, we found that SARS-CoV-2 infection was less common among employees who had received a previous influenza vaccination: relative risk reductions of 37% and 49% were observed following influenza vaccination during the first and second COVID-19 waves, respectively. The quadrivalent inactivated influenza vaccine induced a trained immunity program that boosted innate immune responses against various viral stimuli and fine-tuned the anti-SARS-CoV-2 response, which may result in better protection against COVID-19. Influenza vaccination led to transcriptional reprogramming of monocytes and reduced systemic inflammation. These epidemiological and immunological data argue for potential benefits of influenza vaccination against COVID-19, and future randomized trials are warranted to test this possibility.


Assuntos
COVID-19/imunologia , Proteção Cruzada/fisiologia , Imunidade Inata/fisiologia , Vacinas contra Influenza/administração & dosagem , COVID-19/epidemiologia , COVID-19/prevenção & controle , Citocinas/imunologia , Citocinas/metabolismo , Regulação para Baixo , Imidazóis/imunologia , Incidência , Vacinas contra Influenza/imunologia , Países Baixos/epidemiologia , Recursos Humanos em Hospital , Poli I-C/imunologia , Proteômica , Fatores de Risco , Análise de Sequência de RNA
19.
Anticancer Res ; 41(10): 4867-4874, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34593434

RESUMO

BACKGROUND/AIM: BCR-ABL tyrosine kinase inhibitors (TKIs) are exceptionally effective drugs in the treatment of chronic myeloid leukemia, nevertheless, TKIs have also an effect on platelets. We aimed to investigate the effect of a third-generation TKI, ponatinib on platelet functions. MATERIALS AND METHODS: Collagen-induced platelet aggregation and coated-platelet formation were examined using in vitro and in ex vivo samples of patients on ponatinib therapy. RESULTS: In platelet rich plasma of healthy volunteers, ponatinib at a supra-therapeutic concentration (1,000 nM) significantly impaired collagen induced platelet aggregation (p≤0.01) and reduced the formation of coated-platelets at 150 nM ponatinib concentration (p≤0.05). In addition, upon glycoprotein VI (GPVI) receptor activation, a significantly lower percentage of PAC1 binding platelets (p≤0.05) was observed at 1,000 nM final concentration of ponatinib. Platelets, isolated from patients on ponatinib therapy showed impaired collagen elicited aggregation response, already in pre-dose samples compared to healthy donors. CONCLUSION: The therapeutic concentration of ponatinib impairs platelet activation processes elicited by GPVI receptor agonists.


Assuntos
Antineoplásicos/uso terapêutico , Plaquetas/efeitos dos fármacos , Colágeno/farmacologia , Imidazóis/uso terapêutico , Leucemia Mielogênica Crônica BCR-ABL Positiva/tratamento farmacológico , Agregação Plaquetária/efeitos dos fármacos , Piridazinas/uso terapêutico , Adulto , Idoso , Estudos de Casos e Controles , Feminino , Seguimentos , Humanos , Leucemia Mielogênica Crônica BCR-ABL Positiva/metabolismo , Leucemia Mielogênica Crônica BCR-ABL Positiva/patologia , Masculino , Pessoa de Meia-Idade , Prognóstico
20.
Anal Chim Acta ; 1184: 338984, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34625268

RESUMO

In this study, Zeolitic Imidazole Framework-8/cellulose aerogel (ZIF-8/CA) hybrid was successfully fabricated through a simple doping method and ZIF-8 acted as the major component for adsorption. In order to elucidate the adsorption mechanism deeply, molecular simulation was adopted to the expound the interaction modes between ZIF-8 and the fluoroquinolones (FQs). ZIF-8/CA was used as the adsorbent for semi-automated pipette tip solid phase extraction (PT-SPE). In combination with high performance liquid chromatography tandem fluorescence detector (HPLC-FLD), the established method was successfully employed to determine trace amount of FQs in water samples. Extraction parameters such as the content of ZIF-8, pH of sample solution, volume of sample, flow rate of sampling, type and volume of elution solvent were investigated. Under the optimized conditions, satisfactory linearity was achieved with the correlation coefficient (R2) ranging from 0.9954 to 0.9992. The limits of detection were in the range of 0.337-1.707 ng L-1. And the recoveries varied from 75.9% to 96.8% with RSD less than 8.0%. The established method was demonstrated to be sensitive, efficient and convenient.


Assuntos
Poluentes Químicos da Água , Zeolitas , Celulose , Fluoroquinolonas/análise , Imidazóis , Extração em Fase Sólida , Água , Poluentes Químicos da Água/análise
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