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1.
Environ Pollut ; 302: 118961, 2022 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-35183667

RESUMO

The intestinal microbiota has a key role in human health via the interaction with the somatic and immune cells in the digestive tract environment. Food, through matrix effect, nutrient and non-nutrient molecules, is a key regulator of microbiota diversity. As a food contaminant, the pesticide chlorpyrifos (CPF) has an effect on the composition of the intestinal microbiota and induces perturbation of microbiota. Prebiotics (and notably inulin) are known for their ability to promote an equilibrium of the microbiota that favours saccharolytic bacteria. The SHIME® dynamic in vitro model of the human intestine was exposed to CPF and inulin concomitantly for 30 days, in order to assess variations in both the bacterial populations and their metabolites. Various analyses of the microbiota (notably temporal temperature gradient gel electrophoresis) revealed a protective effect of the prebiotic through inhibition of the enterobacterial (E. coli) population. Bifidobacteria were only temporarily inhibited at D15 and recovered at D30. Although other potentially beneficial populations (lactobacilli) were not greatly modified, their activity and that of the saccharolytic bacteria in general were highlighted by an increase in levels of short-chain fatty acids and more specifically butyrate. Given the known role of host-microbiota communication, CPF's impact on the body's homeostasis remains to be determined.


Assuntos
Clorpirifos , Microbiota , Clorpirifos/toxicidade , Escherichia coli/metabolismo , Humanos , Inulina/metabolismo , Inulina/farmacologia , Prebióticos/análise
2.
Food Res Int ; 159: 111591, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-35940790

RESUMO

Present investigation describes immobilization efficiency of endoinulinase onto hetero-functionalized halloysite nanoclay using 3-aminopropyltriethoxysilane and glutaraldehyde as crosslinkers. Under optimal conditions (APTES 0.75%, sonication time 2.25 h, glutaraldehyde 0.75%, activation-time 65 min, immobilized endoinulinase load 60 IU and coupling-time 1 h), maximum yield in enzyme activity (70.65%) and immobilization (89.61%) was obtained. Developed immobilized biocatalyst shown maximum activity at 65 °C and pH 5.0 with wide range thermal (50-80 °C) and pH (4.0-9.0) stability. Increase in half-life (28.70-fold) of immobilized endoinulinase was observed as compared to free enzyme. An enhanced Km and reduced Vmax of endoinulinase for inulin was recorded after immobilization. Maximum FOSs production 98.42% was obtained, under optimized conditions (inulin 10%; immobilized endoinulinase load 85 IU; hydrolysis-time 10 h and agitation rate 130 rpm) containing kestose (36.26%), nystose (27.02%), fructofuranosylnystose (9.98%) and FOSs DP 5-9 (25.15%). Developed immobilized biocatalyst exhibited a splendid operational stability for 18 batch cycles.


Assuntos
Glicosídeo Hidrolases , Inulina , Argila , Glutaral , Concentração de Íons de Hidrogênio , Oligossacarídeos
3.
Sci Rep ; 12(1): 13401, 2022 Aug 04.
Artigo em Inglês | MEDLINE | ID: mdl-35927320

RESUMO

The current study aimed to figure out the effect of using a combination of 2% inulin, and 2% Fructo-oligosaccharides (FOS) with Lactobacillus acidophilus and their bacteriocin on some yogurt properties such as coagulation time, extending the shelf life of set yogurt and its microbiological quality, also the acceptance by consumers. The results indicated that coagulation time increased by 22.75% in yogurts prepared with Lactobacillus acidophilus and their bacteriocins compared to the control, and titratable acidity increased gradually in all treatments during storage. Hence control acidity (%) increased from 0.84 ± 0.02A at zero time to 1.23 ± 0.03A after 14 days of cold storage, while treatment (T4) was 0.72 ± 0.01C at zero time and reached 1.20 ± 0.5A after 39 days at the same conditions. The sensory properties showed the superiority of inulin, FOS, and Lactobacillus acidophilus bacteriocin groups. Lactobacillus bulgaricus, Streptococcus thermophiles, and Lactobacillus acidophilus count increased in the treatments compared to the control group, with an extended shelf life to 39 days of storage in the medicines containing lactobacillus acidophilus bacteriocin. Coliforms, Moulds, and yeasts did not detect in the treatments comprising 2% inulin, 2% FOS, and lactobacillus acidophilus bacteriocin for 39 days of refrigerated storage. This study proved that 2% inulin, 2% FOS, and Lactobacillus acidophilus bacteriocin fortification extended the shelf life by more than 5 weeks.


Assuntos
Bacteriocinas , Probióticos , Bacteriocinas/farmacologia , Inulina/farmacologia , Lactobacillus acidophilus , Oligossacarídeos/farmacologia , Probióticos/farmacologia , Iogurte/microbiologia
4.
Molecules ; 27(13)2022 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-35807483

RESUMO

Inulin is a popular prebiotic that is often used in the production of ice cream, mainly to improve its consistency. It also reduces the hardness of ice cream, as well as improving the ice cream's organoleptic characteristics. Inulin can also improve the texture of sorbets, which are gaining popularity as an alternative to milk-based ice cream. Sorbets can be an excellent source of natural vitamins and antioxidants. The aim of this study was to evaluate the effect of the addition of inulin on the sensory characteristics and health-promoting value of avocado, kiwi, honey melon, yellow melon and mango sorbets. Three types of sorbets were made-two with inulin (2% and 5% wt.) and the other without-using fresh fruit with the addition of water, sucrose and lemon juice. Both the type of fruit and the addition of inulin influenced the sorbet mixture viscosity, the content of polyphenols, vitamin C, acidity, ability to scavenge free radicals using DPPH reagent, melting resistance, overrun and sensory evaluation of the tested sorbets (all p < 0.05). The addition of inulin had no impact on the color of the tested sorbets, only the type of fruit influenced this feature. In the sensory evaluation, the mango sorbets were rated the best and the avocado sorbets were rated the worst. Sorbets can be a good source of antioxidant compounds. The tested fruits sorbets had different levels of polyphenol content and the ability to scavenge free radicals. Kiwi sorbet had the highest antioxidant potential among the tested fruits. The obtained ability to catch free radicals and the content of polyphenols proved the beneficial effect of sorbets, particularly as a valuable source of antioxidants. The addition of inulin improved the meltability, which may indicate the effect of inulin on the consistency. Further research should focus on making sorbets only from natural ingredients and comparing their health-promoting quality with the ready-made sorbets that are available on the market, which are made from ready-made ice cream mixes.


Assuntos
Sorvetes , Mangifera , Antioxidantes/análise , Frutas/química , Sorvetes/análise , Inulina , Polifenóis/análise
5.
Enzyme Microb Technol ; 160: 110097, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-35835015

RESUMO

The thermostability of enzymes is an essential factor that performs a vital role during practical applications. Inulin fructotransferases can efficiently convert inulin into bio-functional difructose anhydrides (DFAs). The present study aimed to improve the thermostability of a previously reported inulin fructotransferase, SpIFTase, and apply it to the biosynthesis of DFA I. In silico rational design was used to predict mutation sites, based on sequential and structural information. Two triple-site mutants, Q69L/Q234L/K310G and E201I/Q234L/K310G, were characterized and exhibited enhanced thermostability with approximately 5 °C higher in melting temperature (Tm), respectively, and a 45-fold longer half-life (t1/2) at 70 °C, compared to that of SpIFTase. Molecular dynamic simulations and elaborate structural analysis suggested that the combinations of hydrophobic interaction, electrostatic potential distribution, and decreased flexibility via stabilization of loops and α-helix improved the thermostability of SpIFTase. Additionally, the promising mutants exhibited great potential to the industrial production of DFA I.


Assuntos
Hexosiltransferases , Inulina , Dissacarídeos , Estabilidade Enzimática , Hexosiltransferases/genética , Hexosiltransferases/metabolismo , Temperatura
6.
Front Endocrinol (Lausanne) ; 13: 876657, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35784546

RESUMO

Background: Latent autoimmune diabetes in adults (LADA) is a heterogeneous form of diabetes, characterized by autoimmune destruction of pancreatic ß-cells as well as insulin resistance and is triggered by environmental factors in the context of genetic susceptibility. Berberine (BBR), a small alkaloid isolated from medicinal plants, has antidiabetic, anti-inflammatory, and antibacterial effects. Inulin is a common prebiotic that has been shown to improve glycemic control, alter the gut microbiota and suppress inflammation. The primary purpose of this study was to evaluate the effects of oral BBR and inulin combined with insulin therapy on diabetes care in patients with LADA. Methods and Analysis: We will conduct a single-center, prospective, randomized, double-blind, placebo-controlled trial. A total of 240 patients with LADA who have started insulin therapy will be randomly allocated either to the intervention or control group. After a 1-week run-in period, they will complete a 3-month treatment of BBR alone, inulin plus BBR, inulin alone, or placebo. Anthropometric and clinical data will be collected at five time points: baseline, 3 months, 6 months, 9 months, and 12 months from baseline. The primary outcome was the change in glycated hemoglobin levels. Dynamic blood glucose parameters, ß-cell function, and gut microbiota, as well as adverse events and quality of life will be monitored. Discussion: Glycemic control is critical for preventing the progression of diabetes. Although insulin is a recommended treatment for patients with LADA, there are currently no drugs that can effectively prevent the progressive destruction of pancreatic ß-cells or maintain their function. Several studies have found that when berberine and prebiotics are used alone, they have beneficial metabolic effects. This clinical research protocol will assess the efficacy of the combined treatment of berberine plus inulin and provide new ideas for future pharmacological research and clinical practices in diabetes care and glycemic control for LADA patients. Ethics and Dissemination: This study has been approved by the Ethics Committee of National Clinical Research Center of the Second Xiangya Hospital of Central South University (approval number: 2021-046). Clinical Trial Registration: ClinicalTrials.gov, identifier NCT04698330.


Assuntos
Berberina , Diabetes Mellitus Tipo 1 , Intolerância à Glucose , Diabetes Autoimune Latente em Adultos , Adulto , Berberina/uso terapêutico , Diabetes Mellitus Tipo 1/tratamento farmacológico , Humanos , Insulina , Inulina/uso terapêutico , Estudos Prospectivos , Qualidade de Vida , Ensaios Clínicos Controlados Aleatórios como Assunto
7.
Carbohydr Polym ; 294: 119742, 2022 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-35868743

RESUMO

Atractylodes macrocephalon polysaccharides of alleviating weightlessness-induced bone loss (WIBL) are rarely reported. In this study, a neutral polysaccharide fragment named AMP1-1 was isolated and purified. Monosaccharide composition and gel permeation chromatography analysis indicated that AMP1-1 was composed of glucose and fructose with molecular weight of 1.433 kDa. Based on data of gas chromatography-mass spectrometer (GCMS), a linear backbone consisted of α-d-Glcp-(1→ and →1)-ß-d-Fruf-2→ was discovered. Combining results from nuclear magnetic resonance (NMR), the inulin-type fructan AMP1-1 was identified as α-d-Glcp-1→(2-ß-d-Fruf-1)7. Anti-WIBL activity of AMP1-1 was evaluated though analyzation of mechanical properties, BALP and TRAP 5b activities on femur. In vitro mRNA expression indicated that anti-WIBL activity of AMP1-1 was achieved by promote bone formation and inhibit bone resorption in primary osteoblasts and RAW264.7 cell lines under simulated weightlessness. In conclusion, the inulin-type fructan AMP1-1 with α-d-Glcp-1→(2-ß-d-Fruf-1)7 had anti-WIBL activity via remodeling bone homeostasis.


Assuntos
Atractylodes , Atractylodes/química , Frutanos/química , Inulina/química , Inulina/farmacologia , Peso Molecular , Polissacarídeos/química
8.
Carbohydr Polym ; 294: 119777, 2022 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-35868792

RESUMO

Aspidopterys obcordata vine is a Chinese Dai ethnic herb used to treat urolithiasis. However, the material basis and underlying mechanisms remain undefined. In this study, a 2.3 kD inulin-like A. obcordata fructan (AOFOS) was isolated by size exclusion column chromatography and characterized by ultrahigh-performance liquid chromatography-ion trap-time of flight mass spectrometry (UPLC-IT-TOF-MS), nuclear magnetic resonance (NMR) spectroscopy, gas chromatography mass spectrometry (GC-MS) and high-performance gel permeation chromatography (HGPC). In addition, AOFOS showed unique anti-urolithiasis activity in Drosophila kidney stone models. Mechanism study indicated that AOFOS reduced the size of calcium oxalate crystals by inhibiting the formation of large size crystals and the generation rate of calcium oxalate crystals as well as the crystal form conversion from calcium oxalate monohydrate (COM) to calcium oxalate dihydrate (COD).


Assuntos
Cálculos Renais , Malpighiaceae , Oxalato de Cálcio/química , Cristalização , Frutanos , Inulina , Cálculos Renais/química
9.
J Agric Food Chem ; 70(29): 9154-9165, 2022 Jul 27.
Artigo em Inglês | MEDLINE | ID: mdl-35849535

RESUMO

The present study aimed to investigate the effects of fructo-, inulin-, and galacto-oligosaccharides (FOS, IOS, and GOS) on forming the Maillard reaction products such as browning, α-dicarbonyl compounds, and advanced glycation end products (AGEs). The model solutions at pH 6.8 containing each carbohydrate (mono-, di-, and oligosaccharides) and whey protein were incubated at 50 °C for 8 weeks. In the IOS model, sugars of DP3 or larger were significantly decreased at 4 weeks, whereas at 6 weeks in the FOS model. The residual amount of GOS after 8 weeks was higher than FOS and IOS; however, a large amount of 3-deoxyglucosone was formed compared to the other models. Nε-Carboxymethyllysine (CML) concentrations in oligosaccharide models were about half of those in monosaccharide and lactose models. The highest concentrations of glyoxal- and methylglyoxal-derived hydroimidazolones 3 (G-H3 and MG-H3) were observed in the IOS model, indicating the involvement of fructose units linked by ß-2 → 1 bonds. G-H3 and MG-H3 quantification could be a useful indicator to reflect the modification of an arginine residue by fructose if used acid-hydrolysis for AGE analysis. CML, G-H3, and MG-H3 were considerably formed even in the FOS model, which has no reducing terminal site, suggesting that degradation products of oligosaccharides probably participated in the formation of AGEs.


Assuntos
Produtos Finais de Glicação Avançada , Reação de Maillard , Frutose , Produtos Finais de Glicação Avançada/química , Glioxal/química , Inulina , Oligossacarídeos/química , Aldeído Pirúvico/metabolismo , Proteínas do Soro do Leite
10.
Food Res Int ; 158: 111544, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-35840239

RESUMO

The effect of the addition of the prebiotic inulin and free and microencapsulated Bifidobacterium animalis subsp. Lactis (BB-12) strains to synbiotic dry coppa formulations was evaluated during 45 days of ripening. The following formulations were made: control C without probiotic and prebiotic; PROB with free probiotic; SYNB with free probiotic and inulin; ENPROB with microencapsulated probiotic, and ENSYNB with microencapsulated probiotic and inulin. The incorporation of BB-12 with inulin provided adequate physicochemical characteristics (proximate composition, weight loss, pH, water activity (aw), and instrumental color). The treatments PRO and SYNB showed lower lipid and protein oxidation levels. The treatments PROB, SYNB, ENPROB, and ENSYNB had viable cell counts above 109 CFU/g and can be considered probiotic. In the in vitro gastrointestinal simulation, the BB-12 strain showed survival and growth capacity in saline solution and at low pH values for all treatments. The sample SYNB was the most accepted by the assessors in the sensory evaluation. Therefore, dry coppa can be used as a vehicle for the development of a synbiotic fermented meat product.


Assuntos
Bifidobacterium animalis , Probióticos , Simbióticos , Bifidobacterium animalis/metabolismo , Inulina/química , Prebióticos
11.
Sci Rep ; 12(1): 13014, 2022 Jul 29.
Artigo em Inglês | MEDLINE | ID: mdl-35906473

RESUMO

Inulin might improve body composition in obese children. We aimed to determine the effects of inulin supplementation on body composition and metabolic outcomes in obese children. A randomized, double-blinded placebo-controlled study was conducted in obese Thai children aged 7-15 years. Participants were assigned to 3 treatment groups for 6 months: 13 g of extracted inulin powder from Thai Jerusalem artichoke, isocaloric maltodextrin, and dietary fiber advice groups. Body composition was assessed by bioelectrical impedance analysis. One-hundred and fifty-five children completed the study (mean age 10.4 ± 2.2 years, BMI z-score 3.2 ± 1.0, 59% male). The drop-out rate was 6%. The inulin extract yielded more than 90% compliance without significant gastrointestinal side effects. All three groups demonstrated a significant decrease in BMI z-score, fat mass index (FMI), and trunk FMI, but the differences between groups were not observed. Fat-free mass index significantly increased only in the inulin group (16.18 ± 1.90 vs. 16.38 ± 1.98 kg/m2, P = 0.009). There were no significant differences in the metabolic profiles between groups. Despite showing no substantial effect on adiposity, inulin may increase fat-free mass in obese children. Further research in the change of gut microbiota composition is needed to determine inulin's impact on host-microbe interaction in pediatric obesity.


Assuntos
Helianthus , Obesidade Pediátrica , Composição Corporal , Índice de Massa Corporal , Criança , Fibras na Dieta/uso terapêutico , Suplementos Nutricionais , Feminino , Humanos , Inulina/farmacologia , Masculino , Obesidade Pediátrica/tratamento farmacológico
12.
J Agric Food Chem ; 70(30): 9399-9411, 2022 Aug 03.
Artigo em Inglês | MEDLINE | ID: mdl-35881537

RESUMO

Vegetable oils with varying saturated fat levels were inoculated with Lacticaseibacillus rhamnosus GG (LGG), subjected to different heat treatments in the absence and presence of inulin and stored for 12 months at room temperature. After storage, the heat-treated probiotics actively grew to high concentrations after removal of the oils and reculturing. The bacterial samples, regardless of aerobic or anaerobic conditions and treatment methods, showed no changes in their growth behavior. The random amplified polymorphic DNA-polymerase chain reaction, antimicrobial, morphology, and motility tests also showed no major differences. Samples of LGG treated with a higher antioxidant content (Gal400) showed reduced inflammatory and anti-inflammatory properties. These findings have been confirmed by metabolite and genome sequencing studies, indicating that Gal400 showed lower concentrations and secretion percentages and the highest number of single nucleotide polymorphisms. We have shown proof of concept that LGG can be stored in oil with minimum impact on probiotic in vitro viability.


Assuntos
Lactobacillus rhamnosus , Probióticos , Inulina , Óleos Vegetais , Temperatura
13.
BMJ Open ; 12(7): e058875, 2022 07 06.
Artigo em Inglês | MEDLINE | ID: mdl-35793918

RESUMO

INTRODUCTION: This review aims to assess the effects of dietary supplementation with inulin-type fructans (ITF) compared with no supplementation on cardiovascular disease risk factors in adults and assess the quality of trial reporting using the Consolidated Standards of Reporting Trials (CONSORT) and CONSORT for abstract (CONSORT-A) checklists. METHODS AND ANALYSIS: We will search randomised controlled trials (RCTs) in MEDLINE, EMBASE, CINAHL, Emcare, AMED and the Cochrane Database of Systematic Reviews from inception to 31 March 2022, without any language restrictions. The RCTs need to administer ITF in adults for at least 2 weeks and assess effects on at least one cardiovascular risk factor. We will exclude RCTs that (1) assessed the postprandial effects of ITF; (2) included pregnant or lactating participants; (3) enrolled participants undergoing treatment that might affect the response to ITF. We will assess the study risk of bias (RoB) using V.2 of the Cochrane RoB tool for RCTs (RoB 2) and the certainty of the evidence using the Grading of Recommendations, Assessment, Development and Evaluations (GRADE) approach. We will pool data using a random-effects model. We will use the χ2 test to compare compliance of CONSORT and CONSORT-A checklists and Poisson regression to identify factors associated with better reporting. ETHICS AND DISSEMINATION: Ethics approval is not required for secondary analysis of already published data. We will publish the reviews in a peer-review journal. PROSPERO REGISTRATION NUMBER: CRD42019136745.


Assuntos
Doenças Cardiovasculares , Frutanos , Adulto , Doenças Cardiovasculares/prevenção & controle , Frutanos/farmacologia , Frutanos/uso terapêutico , Humanos , Inulina , Metanálise como Assunto , Ensaios Clínicos Controlados Aleatórios como Assunto , Revisões Sistemáticas como Assunto
14.
Food Chem ; 393: 133391, 2022 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-35689919

RESUMO

Bougainvillea glabra bracts extract was encapsulated by freeze drying using the prebiotic fibers polydextrose and inulin as encapsulating materials. The stability and the in vitro gastrointestinal digestion of B. glabra powders were evaluated. High retentions of betacyanins (77-86%) were obtained at 30 °C after 42 days of storage. At 50 °C, took place the highest betacyanins degradation, resulting in retentions ranging from 50 to 64% in the powders containing inulin and polydextrose, respectively. Total phenolic compounds had a final retention of 49-59% and antioxidant capacity varied from 45 to 53% at 50 °C. The betacyanins degradation followed the first-order kinetic and the degradation rates ranged from 0.0030 to 0.0163 days-1. The simulation of gastrointestinal digestion showed a maximum release and stability of betacyanins and phenolics in the gastric phase; however the release was slower in the powders containing inulin. The powders showed great potential as bioactive ingredients in foods.


Assuntos
Betacianinas , Nyctaginaceae , Antioxidantes , Inulina , Fenóis , Extratos Vegetais/metabolismo , Pós
15.
Food Funct ; 13(12): 6737-6748, 2022 Jun 20.
Artigo em Inglês | MEDLINE | ID: mdl-35665791

RESUMO

Dietary fibers such as fructans can protect the intestinal epithelial barrier integrity, but the mechanisms underlying this protection are not completely understood. We aimed to study the protective effect of ß(2→1)-ß(2→6) branched graminan-type fructans (GTFs) on gut epithelial barrier function that was disrupted by three different agents which impact the barrier function via different cellular mechanisms. The effects of GTFs were compared with those of linear ß(2→1) inulin-type fructans (ITFs). T84 intestinal epithelial monolayers were incubated with GTFs and ITFs. Afterwards, the monolayers were challenged with the barrier disruptors calcium ionophore A23187, 12-myristate 13-acetate (PMA) and deoxynivalenol (DON). Transepithelial resistance was measured with an electric cell-substrate impedance sensing system. All fructans studied prevented the barrier disruption induced by A23187. ITF II protected from the disruptive effects of PMA. However, none of the studied fructans influenced the disruption induced by DON. As a measure of disruption-induced inflammation, interleukin-8 (IL-8) production by the intestinal epithelium was determined by ELISA. The production of IL-8 induced by A23187 was decreased by all fructans, whereas IL-8 production induced by DON decreased only upon pre-treatment with ITF II. None of the studied fructans prevented PMA induced IL-8 production. GTFs just like ITFs can influence the barrier function and inflammatory processes in gut epithelial cells in a structure-dependent fashion. These distinct protective effects are dependent on the different signaling pathways that lead to gut barrier disruption.


Assuntos
Agave , Chicória , Agave/metabolismo , Calcimicina/farmacologia , Chicória/metabolismo , Frutanos/farmacologia , Humanos , Interleucina-8/genética , Interleucina-8/metabolismo , Mucosa Intestinal/metabolismo , Inulina/metabolismo , Inulina/farmacologia
16.
Food Res Int ; 156: 111156, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35651022

RESUMO

Until now, although different studies have shown the potential prebiotic effect of seaweed carbohydrates, no studies with the whole seaweeds have been carried out. In addition, the prebiotic effect throughput sequencing remains poorly investigated since most of the published works used qPCR or FISH to estimate bacterial changes. In this work, an in vitro model of the human distal colon was used to determine, for the first time, the potential prebiotic effect of a brown whole seaweed Himanthalia elongata. The whole seaweed was characterized in basis of its nutritional and mineral composition and submitted to the entire gastrointestinal digestion. The prebiotic effect was evaluated by the microbial modulation through 16S rRNA amplicon sequencing, qPCR and short-chain fatty acid analysis. The obtained results indicated that the colonic fraction of H. elongata was used selectively by the Bacteroides genus, more specifically by the specie Bacteoides ovatus, whereas inulin was used mainly by the Parabacteroides genus, being Parabacteroides distasonis the most abundant identified specie. Selective use of inulin by P. distasonis is, therefore, reported by the first time. qPCR analysis shown no significative differences in Bifidobacterium population and a decrease in Lactobacillus along the fermentation assays with both substrates. Regarding to the short-fatty acid production, maximal concentration, 56.11 ± 20.48 mM, was achieved for H. elongata, at 24 h of fermentation whereas for inulin total acid production was 93.66 ± 21.82 mM at 48 h of assay. The metabolic pathways associated with bacterial genera were not significantly different between the two tested substrates. Although more studies are necessary to elucidate the prebiotic character of H. elongata, the results presented in this work are promissory and could open new opportunities of research and application in the area of Nutrition and Food Chemistry.


Assuntos
Besouros , Feófitas , Alga Marinha , Animais , Colo/microbiologia , Humanos , Inulina , Feófitas/química , Prebióticos , RNA Ribossômico 16S/genética , Verduras
17.
Food Res Int ; 156: 111161, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35651028

RESUMO

Algal oil is an aquatic plant source of polyunsaturated fatty acids, especially docosahexaenoic acid (DHA), which may exhibit health benefits when consumed in sufficiently high quantities. But its application as a healthy lipid in functional foods is often limited by its tendency to rapidly oxidize and produce rancid off-flavors. In this study, algal oil-in-water emulsions were prepared using pea protein isolate (PPI)-inulin conjugates as emulsifiers. These conjugates were prepared via the Maillard reaction using a combination of ultrasound and pH-shift treatments. The algal oil phase was mixed with lemon oil (0 to 1.2 wt%) prior to homogenization to improve its sensory characteristics. The addition of 0.6 wt% of lemon oil did not significantly affect the formation and physical stability of the emulsions but it did mask the unpleasant odor of algae oil. The conjugate-stabilized emulsions exhibited shear thinning behavior and had a higher viscosity than PPI-stabilized emulsions. They also had a higher surface load and formed thicker interfacial layers. The conjugates, especially the conjugates prepared by the combined treatments, also significantly improved the physical and oxidative stability of the emulsions. These effects were attributed to an increase in the steric repulsion between the oil droplets due to the hydrophilic inulin chains, as well as to an increase in the antioxidant activity of the interfacial layers due to the presence of more antioxidant proteins. Overall, our results show that the quality attributes and shelf life of DHA-fortified algae oil emulsions can be significantly improved using a plant-based protein-inulin conjugate.


Assuntos
Proteínas de Ervilha , Emulsões , Concentração de Íons de Hidrogênio , Inulina , Reação de Maillard , Estresse Oxidativo , Água
18.
J Environ Manage ; 318: 115525, 2022 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-35724574

RESUMO

A novel nano-adsorbent zinc oxide impregnated graphene oxide/inulin (ZGI) was prepared for the investigation of the removal efficiency of enrofloxacin. Characterization of the nano-adsorbent was accomplished through Fourier transform Infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM) coupled with EDS, transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS) and Raman spectroscopy. The average crystallite size of nanomaterial (ZGI) calculated from XRD data was 14.82 nm. The adsorption of enrofloxacin onto ZGI was performed in batch mode. The variables of adsorption process such as adsorbent dose, pH, contact time and initial concentration of enrofloxacin were optimized by Taguchi method to achieve the maximum removal efficiency. The optimum values of variables were: adsorbent dose = 25 mg, pH = 7, contact time = 60 min and initial concentration = 50 mg/L. The maximum adsorption capacity and removal efficiency of the material for enrofloxacin were 317.83 mg/g and 98.60%, respectively at 303 K. Redlich-Peterson isotherm model was the best fitted among the various isotherm models based on highest R2 values (0.9978-0.9981) and lowest χ2 (3.43 ×10-4-2.00×10-3). Kinetic data followed pseudo-second order model (R2 ≥ 0.9974) more accurately as compared to pseudo-first order model (R2≤0.9772). The adsorption mechanism was illustrated on the basis of XPS and Raman studies. Reusability investigation showed that the nano-adsorbent ZGI could be used up to 5 adsorption-desorption cycles with greater than 90% removal efficiency.


Assuntos
Grafite , Poluentes Químicos da Água , Óxido de Zinco , Adsorção , Enrofloxacina , Grafite/química , Concentração de Íons de Hidrogênio , Inulina , Cinética , Espectroscopia de Infravermelho com Transformada de Fourier , Água , Poluentes Químicos da Água/química , Óxido de Zinco/química
19.
Rev Esc Enferm USP ; 56(spe): e20210440, 2022.
Artigo em Inglês, Português | MEDLINE | ID: mdl-35770890

RESUMO

OBJECTIVE: To evaluate the effect of curcumin on renal function, hemodynamics, and renal oxidative profile of rats with chronic kidney disease (CKD) subjected to renal ischemia-reperfusion injury (IRI). METHODS: Wistar rats, 250-300 g, distributed in four groups: Sham (n = 5), CKD simulation; CKD (n = 5), 5/6 renal ablation for CKD induction; CKD + IRI (n = 5), CKD and renal pedicle clamping for 30 minutes; and CKD + IRI+curcumin (n = 5), CKD + IRI, curcumin administration 30 mg/kg/day, orally, for 10 days. Renal function (inulin clearance, urine flow, plasma creatinine), hemodynamics (blood pressure), and oxidative profile (peroxides, TBARS, and urine nitrate, non-protein soluble thiols in renal tissue) were evaluated. RESULTS: The CKD + IRI + curcumin group showed increased inulin clearance and reduced plasma creatinine, decreased RVR and increased RBF, decreased oxidative metabolites in urine and increased thiols in renal tissue when compared with the CKD + IRI group. CONCLUSION: The treatment with curcumin preserved renal function and hemodynamics of animals with acute CKD, improving oxidative profile, with reduction of oxidants and preservation of antioxidant reserve.


Assuntos
Injúria Renal Aguda , Curcumina , Insuficiência Renal Crônica , Animais , Creatinina , Curcumina/uso terapêutico , Inulina , Isquemia , Ratos , Ratos Wistar , Insuficiência Renal Crônica/complicações , Reperfusão , Compostos de Sulfidrila
20.
Food Funct ; 13(13): 6875-6893, 2022 Jul 04.
Artigo em Inglês | MEDLINE | ID: mdl-35703137

RESUMO

The incidence rate of ulcerative colitis (UC) has increased significantly over the past decades and it places an increasing burden on health and social systems. The current studies on UC implicate a strong correlation between host gut microbiota immunity and the pathogenesis of UC. Meanwhile, more and more functional oligosaccharides have been reported as prebiotics to alleviate UC, since many of them can be metabolized by gut microbiota to produce short-chain fatty acids (SCFAs). The present review is focused on the structure, sources and specific applications of various functional oligosaccharides related to the prevention and treatment of UC. The available evidence for the usage of functional oligosaccharides in UC treatment are summarized, including fructo-oligosaccharides (FOS), galacto-oligosaccharides (GOS), chito-oligosaccharides (COS), alginate-oligosaccharides (AOS), xylooligosaccharides (XOS), stachyose and inulin.


Assuntos
Colite Ulcerativa , Prebióticos , Colite Ulcerativa/tratamento farmacológico , Colite Ulcerativa/prevenção & controle , Fezes , Humanos , Inulina/química , Inulina/uso terapêutico , Oligossacarídeos/metabolismo
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