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1.
Zhong Nan Da Xue Xue Bao Yi Xue Ban ; 41(1): 65-70, 2016 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-26819427

RESUMO

OBJECTIVE: To investigate the incidence, risk factors and prognosis for contrast-induced acute kidney injury (CI-AKI) according to ESUR and KDIGO criteria in patients undergoing angiography.
 METHODS: We evaluated 260 patients undergoing angiography and/or intervention therapy from April 2011 to January 2012 in the Second Xiangya Hospital of Central South University. All patients received low-osmolality contrast agent (ioversol). Serum creatinine was measured before angiography or at 48 or 72 h after procedure. The multivariate logistic regression was used to analyze the risk factors of CI-AKI. The major adverse events were observed in a year of follow-up.
 RESULTS: Among the 260 patients, 23 experienced CI-AKI and the incidence was 8.8% according to ESUR criteria. Twelve patients experienced CI-AKI and the incidence was 4.6% according to KDIGO criteria. The multivariate logistic regression analysis showed that diabetes mellitus and dehydration were the independent risk factors for CI-AKI according to ESUR criteria; In another KDIGO criteria, chronic kidney disease (CKD), hypercholesterolemia and diabetes mellitus were the independent risk factors for CI-AKI. The prognosis study showed that the mortality of patients with CI-AKI were significantly higher than those without CI-AKI (P<0.05).
 CONCLUSION: The incidence of CI-AKI is associated with diagnostic criteria. Diabetes mellitus, CKD, dehydration and hypercholesterolemia were the independent risk factors for CI-AKI. CI-AKI is a relevant factor for mortality in a year after angiography and/or intervention therapy.


Assuntos
Injúria Renal Aguda/induzido quimicamente , Injúria Renal Aguda/diagnóstico , Meios de Contraste/efeitos adversos , Iodoperaceto/efeitos adversos , Injúria Renal Aguda/mortalidade , Angiografia , Desidratação/epidemiologia , Diabetes Mellitus/epidemiologia , Humanos , Incidência , Modelos Logísticos , Prognóstico , Insuficiência Renal Crônica/epidemiologia , Fatores de Risco
2.
Braz. j. microbiol ; 43(1): 126-134, Jan.-Mar. 2012. ilus, tab
Artigo em Inglês | LILACS | ID: lil-622797

RESUMO

A fractional factorial design 2(5-1) was used to evaluate the effect of temperature, pH, and concentrations of yeast extract, tryptone and Nitsch's trace elements on the biomass, total carotenoids and protection against singlet oxygen by carotenoid extracts of the bacterium Thermus filiformis. In addition, the carotenoid composition was determined by high-performance liquid chromatography connected to a diode array and mass spectrometer detectors (HPLC-DAD-MS/MS). The production of biomass ranged from 0.113 to 0.658 g/L, the total carotenoid from 137.6 to 1,517.4 mg/g and the protection against singlet oxygen from 4.3 to 85.1 %. Results of the fractional factorial design showed that temperature had a negative effect on biomass production and a positive effect on carotenoid content and protection against singlet oxygen, besides, high levels of pH value, concentrations of yeast extract and tryptone had a positive effect on biomass production only at lower temperatures. The main carotenoids of T. filiformis were thermozeaxanthins. In the tested conditions, changes in the levels of the variables influenced the biomass, carotenoid production, and protection against singlet oxygen, although they did not influence the carotenoid profile. The results of this study provide a better understanding on the interactions among certain nutritional and cultivation conditions of a thermophile bacterium, Thermus filiformis, on biomass and carotenoid amounts, as well as on the antioxidant capacity.


Assuntos
Biomassa , Cromatografia Líquida , Carotenoides/análise , Iodoperaceto/análise , Oxigênio Singlete/análise , Thermus/genética , Thermus/isolamento & purificação , Leveduras , Técnicas de Química Combinatória , Concentração de Íons de Hidrogênio , Métodos
3.
Inorg Chem ; 49(4): 1301-3, 2010 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-20070119

RESUMO

A new class of coumarin-based iridium tris-cyclometalated complexes has been developed. These complexes are highly emissive, with emission colors ranging from green to orange-red. Besides modification of ligand structures, color tuning was realized by incorporation of ligands with different electrochemical properties in a heteroleptic structure. The organic light-emitting diodes (OLEDs) using these compounds as emissive dopants are highly efficient and stable. Unlike other Ir(III) phosphorescent dopants, these coumarin-based Ir(III) dopants can effectively trap and transport electrons in the emissive layer.


Assuntos
Cumarínicos/química , Elétrons , Iodoperaceto/química , Irídio/química , Luz , Medições Luminescentes/métodos , Estrutura Molecular , Fotoquímica/métodos , Espectrometria de Fluorescência , Termodinâmica
4.
J Nucl Med ; 45(1): 80-5, 2004 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-14734677

RESUMO

UNLABELLED: Recently, complementary DNA (cDNA) encoding a p-aminohippurate (PAH) transporter designated rat organic anion transporter 1 (OAT1) was isolated. OAT1, a multispecific organic anion transporter at the basolateral membrane, is exclusively expressed in the middle segment of the proximal tubule in the rat kidney. It has been proposed that OAT1 is indirectly involved in PAH uptake via the Na(+) dicarboxylate cotransporter. In this study, in molecular biologic experiments using OAT1-expressing Xenopus laevis oocytes, we obtained evidence that (99m)Tc-mercaptoacetylglycylglycylglycine (MAG3) is transported via OAT1. METHODS: Capped OAT1 complementary RNA (cRNA) was synthesized from library plasmid cDNA linearized with BamHI using in vitro transcription. Defolliculated oocytes were injected with 10 ng of OAT1 cRNA. Two to 3 d after injection, uptake of (99m)Tc-MAG3 was measured using ND96 solution containing 18.5 kBq of (99m)Tc-MAG3. Before the uptake experiments, OAT1-expressing oocytes were preincubated for 2 h with 1 mmol/L glutarate (a dicarboxylate), to generate an outwardly directed glutarate gradient. Then, after incubation for 60 min at room temperature, radioactivity of oocytes was determined. For the inhibition experiments, uptake was assessed in the absence or presence of inhibitor: 2 mmol/L of PAH, o-iodohippurate (OIH), probenecid, 3,5-diiodo-4-pyridone-N-acetate (iodopyracet), furosemide, ethacrynic acid, glucoheptonate, maleic acid, L-Tyr, or tetraethylammonium (TEA) or 0.1 mmol/L of 2,4-dinitrophenol (DNP). RESULTS: Na(+) had a significant effect on (99m)Tc-MAG3 uptake (P < 0.05). Accumulated glutarate stimulated simultaneous (99m)Tc-MAG3 uptake and glutarate excretion (P < 0.001). The following compounds significantly inhibited (99m)Tc-MAG3 uptake: PAH, 8.5% +/- 16.2% of (99m)Tc-MAG3 uptake in the absence of an inhibitor; OIH, 26.4% +/- 21.7%; probenecid, 29.1% +/- 12.4%; iodopyracet, 15.8% +/- 7.9%; furosemide, 30.5% +/- 15.7%; ethacrynic acid, 21.6% +/- 10.6%; glucoheptonate, 35.6% +/- 22.6%; and maleic acid, 60.1% +/- 18.7%. (99m)Tc-MAG3 accumulation in Xenopus laevis oocytes was not significantly inhibited by TEA, L-Tyr, or DNP. CONCLUSION: The following substances had a cis-inhibitory effect on (99m)Tc-MAG3 transport: PAH, OIH, probenecid, iodopyracet, furosemide, ethacrynic acid, and glucoheptonate. Glutarate had a trans-stimulative effect on (99m)Tc-MAG3 transport. (99m)Tc-MAG3 acts as a substrate of OAT1, an organic anion/dicarboxylate exchanger.


Assuntos
Oócitos/metabolismo , Canais de Potássio/metabolismo , Sódio/metabolismo , Tecnécio Tc 99m Mertiatida/farmacocinética , Animais , Transporte Biológico Ativo/efeitos dos fármacos , Transporte Biológico Ativo/fisiologia , Células Cultivadas , Furosemida/farmacologia , Iodoperaceto/farmacologia , Taxa de Depuração Metabólica , Oócitos/diagnóstico por imagem , Oócitos/efeitos dos fármacos , Canais de Potássio/efeitos dos fármacos , Probenecid/farmacologia , Técnica de Diluição de Radioisótopos , Cintilografia , Ratos , Proteínas Recombinantes/metabolismo , Sódio/farmacologia , Xenopus laevis
7.
Eksp Klin Farmakol ; 57(6): 53-4, 1994.
Artigo em Russo | MEDLINE | ID: mdl-7756965

RESUMO

The intravenous injection of cimetidine in a dose of 20 mg/kg enhanced verografine and iodamide excretion in chronic canine experiments. The higher verografine and iodamide excretion was due to their increased renal tubular secretion. In dogs, cimetidine unchanged the secretion of cardiotrast, a test agent for anionic transport. Possible extrarenal mechanisms of action of cimetidine on verografine and iodamide transport were also examined.


Assuntos
Cimetidina/farmacologia , Diatrizoato de Meglumina/metabolismo , Iodamida/metabolismo , Rim/efeitos dos fármacos , Animais , Cimetidina/administração & dosagem , Diatrizoato de Meglumina/análise , Diurese/efeitos dos fármacos , Cães , Interações Medicamentosas , Taxa de Filtração Glomerular/efeitos dos fármacos , Iodamida/análise , Iodoperaceto/análise , Iodoperaceto/metabolismo , Rim/metabolismo , Fatores de Tempo
8.
J Pharmacobiodyn ; 13(3): 179-85, 1990 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-2374087

RESUMO

To predict quantitatively drug interaction kinetics from the single-drug clearance studies, we examined the effect of iodopyracet (IOD) on sulfamethizole (SMZ) excretion in rabbits. Even though the decline of systemic IOD plasma concentration was linear, the renal clearance of SMZ decreased significantly in the presence of IOD. The results could be described by a perfusion model incorporated with the competitive inhibition for tubular secretion. For IOD with a high extraction ratio, it was suggested that a heavy load of the drug was supplied to the sites of secretion and caused the saturation of transport systems, even though the renal excretion kinetics were apparently linear in respect to the systemic circulation. These facts indicated that a linear relationship between the concentrations in the systemic circulation and at the sites of tubular secretion can not always be presumed. Consequently, SMZ-IOD interaction study stressed the importance of the drug concentrations at the sites of interaction for quantitative elucidation of drug-drug interactions.


Assuntos
Iodoperaceto/farmacologia , Córtex Renal/metabolismo , Sulfametizol/farmacocinética , Sulfatiazóis/farmacocinética , Animais , Interações Medicamentosas , Técnicas In Vitro , Córtex Renal/efeitos dos fármacos , Masculino , Taxa de Depuração Metabólica/efeitos dos fármacos , Modelos Biológicos , Coelhos
9.
J Pharmacobiodyn ; 13(2): 97-107, 1990 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-2384854

RESUMO

In order to quantify the renal handling of iodopyracet (IOD) and sulfamethizole (SMZ), single-drug clearance studies in rabbits were performed under quasi-steady state conditions with stepwise increasing the infusion rate of IOD or SMZ. Although concentration dependence of plasma protein binding was observed for both drugs, the urinary excretion rate of IOD was proportional to its total plasma concentration at low total plasma concentrations of 0.05-0.8 mM. On the other hand, the relationship between urinary excretion rate and total plasma concentration of SMZ was a concave-ascending curve at low plasma concentrations and the renal clearance of SMZ was sensitive to changes in plasma protein binding. However, renal clearances referenced to unbound plasma concentration at total plasma concentrations of 0.05 mM for IOD and SMZ were 9.5 and 38 l/h, respectively. Those values were much greater than the effective plasma flow in rabbits. These facts indicated that the intrinsic clearances at the sites of tubular secretion were high and that the rates of secretion were fully or partially limited by the renal plasma flow. Furthermore it was suggested that unbound drug was liberated from plasma protein at the sites of tubular secretion. The data obtained at high plasma concentrations indicated that the tubular secretion of IOD had capacity limited characteristics and that the urinary excretion of SMZ involved tubular reabsorption as well as saturable tubular secretion. From the data obtained, a perfusion-limited pharmacokinetic model was constructed characterizing the excretory processes, namely, glomerular filtration, passive tubular reabsorption, saturable tubular secretion and reequilibrium between bound and unbound drugs in plasma. For both drugs, the estimates for bulk flow rate were reasonable values of effective renal plasma flow and the dissociation constants for tubular secretion agreed well with those for in vitro renal cortex accumulation, suggesting that the kinetic model based on physiological concepts was useful for the understanding of the drug elimination processes.


Assuntos
Iodoperaceto/farmacocinética , Rim/metabolismo , Coelhos/metabolismo , Sulfametizol/farmacocinética , Sulfatiazóis/farmacocinética , Animais , Proteínas Sanguíneas/metabolismo , Córtex Renal/anatomia & histologia , Glomérulos Renais/fisiologia , Túbulos Renais/metabolismo , Modelos Biológicos , Perfusão , Ligação Proteica , Sulfametizol/sangue , Sulfametizol/urina
10.
Farmakol Toksikol ; 53(1): 62-4, 1990.
Artigo em Russo | MEDLINE | ID: mdl-2328806

RESUMO

In chronic experiments on dogs it was shown that verografin and iodamide are excreted from the body not only by filtration but by tubular excretion as well. The maximal transport of verografin and iodamide is significantly lower than that of cardiotrast. In experiments on rats similar results were obtained. Concurrent administration of verografin and iodamide with cardiotrast decreases their excretion in the urine in rats that is probably due to competition for the common transport system in the epithelium of renal tubules.


Assuntos
Diatrizoato de Meglumina/farmacocinética , Iodamida/farmacocinética , Iodobenzoatos/farmacocinética , Iodoperaceto/farmacocinética , Rim/metabolismo , Animais , Transporte Biológico/efeitos dos fármacos , Diatrizoato de Meglumina/análise , Cães , Interações Medicamentosas , Inulina/farmacocinética , Iodamida/análise , Iodoperaceto/análise , Rim/efeitos dos fármacos , Ratos
11.
Biull Eksp Biol Med ; 107(3): 264-6, 1989 Mar.
Artigo em Russo | MEDLINE | ID: mdl-2713458

RESUMO

The effect of a new immunomodulator taktivin (7-20 micrograms/kg subcutaneously, for 6 days) on the diuresis and tubular transport of cardiotrast (diodrast) was studied on rats. taktivin was shown to increase the tubular transport of the xenobiotic without significant changes in glomerular filtration and renal excretion of water, sodium, potassium, uric acid and creatinine. Possible mechanism of taktivin's action on the tubular transport of xenobiotics is discussed.


Assuntos
Adjuvantes Imunológicos/farmacologia , Diurese/efeitos dos fármacos , Iodoperaceto/farmacocinética , Túbulos Renais/efeitos dos fármacos , Peptídeos/farmacologia , Extratos do Timo/farmacologia , Animais , Transporte Biológico/efeitos dos fármacos , Taxa de Filtração Glomerular/efeitos dos fármacos , Túbulos Renais/fisiologia , Ratos , Fatores de Tempo
12.
Biopharm Drug Dispos ; 10(2): 137-52, 1989.
Artigo em Inglês | MEDLINE | ID: mdl-2706315

RESUMO

Plasma kinetics and renal excretion of iodopyracet (3.0 g, administered i.v.) with and without concomitant administration of probenecid were studied in the beagle dog. Pharmacokinetic analysis revealed that tubular secretion is the predominant route of excretion, and that secretion is inhibited by probenecid. A physiologically based kidney model is proposed comprising all the functional characteristics of the kidney that determine the excretion of iodopyracet, i.e. renal plasma flow, urine flow, protein binding, glomerular filtration, tubular secretion, and tubular accumulation. The model enabled an accurate description and analysis of the measured plasma levels and renal excretion rates. Renal clearance of iodopyracet is characterized by supply-limited elimination at low plasma concentrations and capacity-limited elimination at high plasma levels. The interaction with probenecid could be adequately described with the model by competitive inhibition of the carrier-mediated uptake of iodopyracet into the tubular cells. Model calculations showed that in the control experiments tubular secretion was accompanied by a pronounced accumulation of iodopyracet within the cells, which was clearly diminished in the presence of probenecid.


Assuntos
Iodoperaceto/farmacocinética , Probenecid/farmacologia , Animais , Proteínas Sanguíneas/metabolismo , Cães , Interações Medicamentosas , Meia-Vida , Iodoperaceto/urina , Rim/metabolismo , Masculino , Modelos Biológicos , Ligação Proteica
13.
Farmakol Toksikol ; 52(1): 48-50, 1989.
Artigo em Russo | MEDLINE | ID: mdl-2707421

RESUMO

Administration of cardiotrast (50-80 mg twice a day subcutaneously) during pregnancy was shown to induce a significant suppression of tubular secretion of the xenobiotic in the offspring. When cardiotrast was given only in the postnatal period, tolerance failed to develop.


Assuntos
Iodoperaceto/metabolismo , Túbulos Renais/metabolismo , Prenhez/efeitos dos fármacos , Animais , Depressão Química , Diurese/efeitos dos fármacos , Feminino , Iodoperaceto/farmacologia , Túbulos Renais/efeitos dos fármacos , Gravidez , Ratos , Fatores de Tempo
14.
Biull Eksp Biol Med ; 104(11): 602-3, 1987 Nov.
Artigo em Russo | MEDLINE | ID: mdl-3676500

RESUMO

A new inducer of the monooxygenase system zixoryn (oral dose--100 mg/kg, for 4 days) increased the excretion and maximum tubular transport of cardiotrast (diodrast) in rats. Zixoryn had no effect on daily and water diuresis and renal excretion of sodium, potassium and creatinine.


Assuntos
Compostos Benzidrílicos/farmacologia , Diurese/efeitos dos fármacos , Iodoperaceto/metabolismo , Rim/metabolismo , Animais , Indução Enzimática/efeitos dos fármacos , Ratos
16.
Tsitologiia ; 29(3): 309-14, 1987 Mar.
Artigo em Russo | MEDLINE | ID: mdl-3495918

RESUMO

Numerous granules are formed in frog erythrocytes under the influence of cardiotrast (C) (diethanolamine-3,5-diiodo-4-pyridone-1-acetic acid). However, as revealed by cytospectrophotometric investigation and X-ray microanalysis, no C was accumulated in these granules. It is known that C can dissociate into diethanolamine and 3,5-diiodo-4-pyridone-1-acetic acid. It was assumed that under the influence of C granule formation may occur at the expense of an uncharged diethanolamine form penetrating into lysosome-like structures to be accumulated by protonation process. Diethanolamine was found to provoke granule formation in frog erythrocytes. It is impossible to reveal substance in granules because it is colourless and has no ultraviolet absorbtion band. Under the influence of some inhibitors of energy metabolism on granule formation and the granules formed, their inhibitory effect is exerted on the process of granule formation. In granules isolated by differential centrifugation activity of some marker lysosomal enzymes was found which enabled us to attribute these granules to lysosome-like structures.


Assuntos
Meios de Contraste/farmacologia , Grânulos Citoplasmáticos/efeitos dos fármacos , Eritrócitos/efeitos dos fármacos , Iodoperaceto/farmacologia , Animais , Citofotometria , Grânulos Citoplasmáticos/enzimologia , Grânulos Citoplasmáticos/ultraestrutura , Eritrócitos/enzimologia , Eritrócitos/ultraestrutura , Etanolaminas/farmacologia , Lisossomos/enzimologia , Rana temporaria , Espectrometria por Raios X , Espectrofotometria
18.
Biull Eksp Biol Med ; 100(11): 584-6, 1985 Nov.
Artigo em Russo | MEDLINE | ID: mdl-4063508

RESUMO

Excretion of cardiotrast (diotrast) was used to study tubular secretion of organic compounds in rats. Immunostimulants prodigiosin (0.05 mg/kg) and levamisole (10 mg/kg) injected subcutaneously three times every other day increased cardiotrast excretion. High levamisole dose (50 mg/kg), having no stimulating effect on the immunity, failed also to influence tubular cardiotrast transport. Possible mechanisms of immunostimulants' action on the tubular secretion of xenobiotics is discussed.


Assuntos
Adjuvantes Imunológicos/farmacologia , Túbulos Renais/metabolismo , Levamisol/farmacologia , Polissacarídeos Bacterianos/farmacologia , Prodigiozan/farmacologia , Animais , Iodoperaceto/urina , Túbulos Renais/efeitos dos fármacos , Ratos
19.
West J Med ; 142(5): 657-64, 1985 May.
Artigo em Inglês | MEDLINE | ID: mdl-4013281

RESUMO

Review of the literature concerning contrast-induced renal dysfunction shows that the currently used agents are remarkably safe with careful patient selection. Clinically apparent kidney failure after their use is essentially nonexistent in those without preexistent renal insufficiency. The incidence rises rapidly in those with azotemia from any cause, however, and diabetic persons with nephropathy are perhaps at special risk. Vigorous volume expansion is possibly effective as a preventive measure and may attenuate adverse effects in those in whom postcontrast dysfunction occurs. New agents are becoming available. It is not yet known if these will prove safer or cost-effective. They have some experimentally demonstrated and theoretic advantages over the presently used agents.


Assuntos
Injúria Renal Aguda/induzido quimicamente , Meios de Contraste/efeitos adversos , Iodoperaceto/análogos & derivados , Urografia , Feminino , Humanos , Iodoperaceto/efeitos adversos , Pessoa de Meia-Idade
20.
Br J Surg ; 71(10): 799-801, 1984 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-6548397

RESUMO

We have performed a prospective study on 117 patients in order to assess the value of a single contrast water soluble enema in acute large bowel obstruction. In 99 cases where the plain films suggested a diagnosis of mechanical obstruction, the Diodone enema confirmed its presence in 52, and relocated its site in 11. There was free flow of contrast to the caecum in the remaining 35, 11 of whom were shown to have idiopathic pseudo-obstruction. The plain films suggested a diagnosis of pseudo-obstruction in 18 patients. The Diodone enema confirmed this diagnosis in 15, but revealed an unsuspected mechanical obstruction in two. The examination failed in two cases. We conclude that the water soluble single contrast enema is of value in the management of large bowel obstruction. It will confirm the diagnosis of mechanical obstruction and prevent unnecessary surgery in patients with pseudo-obstruction.


Assuntos
Enema , Obstrução Intestinal/diagnóstico por imagem , Intestino Grosso/diagnóstico por imagem , Iodoperaceto , Doença Aguda , Adulto , Idoso , Diagnóstico Diferencial , Feminino , Humanos , Pseudo-Obstrução Intestinal/diagnóstico por imagem , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Radiografia , Solubilidade
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