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1.
J Clin Pediatr Dent ; 47(1): 58-66, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36627221

RESUMO

This research aimed to assess the efficacy of different irrigants applied with different types of needle tips on smear layer removal (SLR) in primary incisors. This study was carried out with 35 freshly extracted upper primary incisors. The samples were randomly distributed to five study groups (n = 7) (1 to 4: experimental, 5: control). These included Group 1: 5% Ethylenediaminetetraacetic Acid (EDTA) + 1% Sodium Hypochlorite (NaOCl) applied with open-ended needle (OEN), Group 2: 6% Citric Acid (CA) + 1% NaOCl applied with OEN, Group 3: 5% EDTA + 1% NaOCl applied with double side-vented needle (DSVN), Group 4: 6% CA + 1% NaOCl applied with DSVN and Group 5: 1% NaOCl applied with OEN. Accordingly, the inner root surfaces were examined using scanning electron microscopy (SEM). The differences between the groups were analyzed using Kruskal-Wallis, Friedman and Siegel-Castellan tests (p < 0.05). In the coronal third, all the experimental groups (groups 1, 2, 3 and 4) were superior to the control group (p = 0.002, p = 0.002, p < 0.001 and p < 0.001, respectively). Groups 2, 3 and 4 showed superior SLR to the control group (p = 0.024, p = 0.001 and p = 0.004, respectively) in the middle third. DSVN groups of EDTA and CA showed superior SLR efficacy than the control (p < 0.001 and p = 0.002, respectively) in the apical third. The SLR efficacy was higher in the coronal third compared to the apical third in the experimental groups (groups 1, 2, 3 and 4) (p = 0.015, p = 0.048, p = 0.048 and p = 0.048, respectively). In addition, 3 samples of EDTA showed erosion (2 in the coronal with OEN, 1 in the middle with DSVN). It was possible to conclude that the SLR efficacy of DSVNs and OENs was similar. CA could be recommended since it did not cause erosive damage compared to EDTA in primary incisors.


Assuntos
Camada de Esfregaço , Humanos , Ácido Cítrico , Cavidade Pulpar , Ácido Edético , Microscopia Eletrônica de Varredura , Irrigantes do Canal Radicular , Preparo de Canal Radicular , Hipoclorito de Sódio/farmacologia
2.
Lasers Med Sci ; 38(1): 30, 2023 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-36595139

RESUMO

The aim of this study was to examine the effectiveness of different irrigation activation methods on smear layer removal and tubular penetration. One hundred-five distal roots of mandibular molar teeth in total; 50 for smear removal efficiency (n = 10) analysis using scanning electron microscopy (SEM), and 55 roots were used to examine tubular penetration using confocal laser scanning microscope (CLSM). Five different irrigation activation methods were used in this study; conventional needle irrigation (CNI), sonic irrigation device of EDDY, passive ultrasonic irrigation (PUI), PIPS and SWEEPS techniques, which are two different laser irrigation activation methods. The obtained data were statistically analyzed and the significance level was determined as p < 0.05. At the apical level, the cleanest canal walls were observed when laser methods PIPS and SWEEPS were used, while in the middle third, there was no difference in smear removal efficiencies between all groups except for the CNI (p > 0.05). Penetration depths and percentages increased from apically to coronally in all groups. The PUI and EDDY generally showed similar penetration depths and percentages to the CNI, except at the coronal root level (p > 0.05). In all groups, when PIPS was used, it showed greater penetration depth and percentage (p < 0.05). PIPS and SWEEPS techniques showed lowest and similar smear scores compared to PUI and EDDY in the apical area where access and effectiveness of the irrigation solution are difficult.


Assuntos
Preparo de Canal Radicular , Camada de Esfregaço , Humanos , Preparo de Canal Radicular/métodos , Ultrassom , Irrigantes do Canal Radicular , Irrigação Terapêutica/métodos , Lasers , Cavidade Pulpar
3.
Biomed Res Int ; 2023: 8858283, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36685672

RESUMO

Irrigation of root canal system is of great significance to the success of endodontic treatment, where sodium hypochlorite (NaOCl) is the most widely used irrigant in chemical preparation. NaOCl functions by eliminating bacterial biofilms and dissolving organic tissue, which may vary according to several factors such as the microbiology of root canal infection and the concentration of the irrigant. It has been proposed that the effectiveness of NaOCl could be enhanced via several methods, including heating the irrigant, applying in conjunction with certain reagents, or activating by agitation techniques. Despite its antibacterial and tissue-dissolving capacities, NaOCl should be used with caution to avoid detrimental effect due to its cytotoxicity and negative effect on dentin properties. In this narrative review, we discussed the factors that affect the properties of NaOCl, the methods to improve its efficacy, and the side effects that might occur in clinical practice.


Assuntos
Preparo de Canal Radicular , Hipoclorito de Sódio , Hipoclorito de Sódio/química , Preparo de Canal Radicular/métodos , Cavidade Pulpar , Irrigantes do Canal Radicular/farmacologia , Irrigantes do Canal Radicular/química , Tratamento do Canal Radicular , Dentina
4.
Sci Rep ; 13(1): 1206, 2023 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-36681702

RESUMO

This study devised a dual-species biofilm model to investigate bacteria, lipoteichoic acid (LTA), and lipopolysaccharide (LPS) simultaneously, and compared the efficacy of conventional and contemporary disinfection protocols. Seventy single-rooted mandibular premolars were included. Fourteen teeth were negative control, and 56 teeth were infected with 3-week-old E. faecalis and E. coli GFP biofilm. Fourteen/56 teeth were positive control, with seven teeth processed for CLSM analysis and seven teeth sampled with paper points (PPs) and cryogenically ground for bacterial, LTA, and LPS analyses. Forty-two teeth were randomly divided into three groups: GWS (GentleWave system) + MIT (minimally invasive technique), PUI (passive ultrasonic irrigation) + CIT (conventional instrumentation technique), and XP-EF (XP-endo Finisher) + CIT (All, n = 14). Samples were collected before (s1) and after disinfection (s2) with PPs and after cryogenically ground (s3). CFUs were counted, and LTA and LPS were quantified with LTA-ELISA and LAL assay, respectively. XP-EF was as effective as PUI (p > 0.05). GWS + MIT was the most effective disinfection protocol against bacteria, LTA, and LPS (p < 0.05). In conclusion, PUI, XP-EF, and GWS were highly effective against bacteria, LTA, and LPS, with GWS being the most effective.


Assuntos
Lipopolissacarídeos , Preparo de Canal Radicular , Bactérias , Cavidade Pulpar , Desinfecção/métodos , Escherichia coli , Irrigantes do Canal Radicular , Preparo de Canal Radicular/métodos , Hipoclorito de Sódio
5.
BMC Oral Health ; 23(1): 20, 2023 01 13.
Artigo em Inglês | MEDLINE | ID: mdl-36639767

RESUMO

BACKGROUND: Inter-appointment medication of the root canals with appropriate intracanal medicaments has been advocated to improve root canal disinfection. Graphene oxide (GO) has shown promising antimicrobial activity against a wide range of microorganisms, besides the capability of carrying antibiotics. The current study aimed to compare the antibacterial activity of double antibiotic paste (DAP) and GO per se and in combination (GO-DAP) against Enterococcus faecalis (E. faecalis). METHODS: A total of 108 extracted human mandibular premolars were contaminated with three-week-old E. faecalis and subjected to a primary microbial assessment. The samples were categorized into 15 groups concerning the intracanal medicament (DAP, GO, GO-DAP, and control) and contact time (1, 7, and 14 days). Then, the root canals were medicated, incubated, and resubjected to a secondary antimicrobial evaluation. The colony-forming units (CFU) were counted to calculate the antimicrobial efficacy. The data were analyzed via the Kruskal-Wallis test (α = 0.05). RESULTS: GO-DAP was the only medicament that completely eradicated E. faecalis in 1 day. The percentage reduction of CFU/ml in the GO-DAP and DAP groups was higher than that in the GO group at all allocated contact times. Furthermore, a significant decrease of the CFU/ml was seen in the GO and DAP groups after 7 and 14 days of being medicated (P < 0.05). CONCLUSION: Since GO-DAP improved root canal disinfection, this novel material can be introduced as a promising intracanal medicament against E. faecalis even in the short run.


Assuntos
Antibacterianos , Anti-Infecciosos , Humanos , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Cavidade Pulpar/microbiologia , Enterococcus faecalis , Irrigantes do Canal Radicular/farmacologia , Irrigantes do Canal Radicular/uso terapêutico
6.
Beijing Da Xue Xue Bao Yi Xue Ban ; 55(1): 38-43, 2023 Feb 18.
Artigo em Chinês | MEDLINE | ID: mdl-36718687

RESUMO

OBJECTIVE: To construct a model of Enterococcus faecalis (E. faecalis) infection in dentinal tubules by gradient centrifugation and to evaluate the antibacterial effect of low-temperature plasma on E. faecalis in dentinal tubules. METHODS: Standard dentin blocks of 4 mm×4 mm×2 mm size were prepared from single root canal isolated teeth without caries, placed in the E. faecalis bacterial solution, centrifuged in gradient and incubated for 24 h to establish the model of dentinal tubule infection with E. faecalis. The twenty dentin blocks of were divided into five groups, low-temperature plasma jet treatment for 0, 5 and 10 min, calcium hydroxide paste sealing for 7 d and 2% chlorhexidine gel sealing for 7 d. Scanning electron microscopy and confocal laser scanning microscope were used to assess the infection in the dentinal tubules and the antibacterial effect of low-temperature plasma. RESULTS: The results of scanning electron microscopy and confocal laser scanning microscopy showed that after 24 h of incubation by gradient centrifugation, E. faecalis could fully enter the dentinal tubules to a depth of more than 600µm indicating that this method was time-saving and efficient and could successfully construct a model of E. faecalis infection in dentinal tubules. Low-temperature plasma could enter the dentinal tubules and play a role, the structure of E. faecalis was still intact after 5 min of low-temperature plasma treatment, with no obvious damage, and after 10 min of low-temperature plasma treatment, the surface morphology of E. faecalis was crumpled and deformed, the cell wall was seriously collapsed, and the normal physiological morphology was damaged indicating that the majority of E. faecalis was killed in the dentinal tubules. The antibacterial effect of low-temperature plasma treatment for 10 min exceeded that of the calcium hydroxide paste sealing for 7 d and the 2% chlorhexidine gel sealing for 7 d. These two chemicals had difficulty entering deep into the dentinal tubules, and therefore only had a few of antibacterial effect on the bacterial biofilm on the root canal wall, and there was also no significant damage to the E. faecalis bacterial structure. CONCLUSION: Gradient centrifugation could establish the model of E. faecalis dentin infection successfully. Low-temperature plasma treatment for 10 min could kill E. faecalis in dentinal tubules effectively, which is superior to the calcium hydroxide paste sealing for 7 d and the 2% chlorhexidine gel sealing for 7 d.


Assuntos
Hidróxido de Cálcio , Clorexidina , Clorexidina/farmacologia , Hidróxido de Cálcio/farmacologia , Enterococcus faecalis/fisiologia , Temperatura , Dentina , Biofilmes , Antibacterianos/farmacologia , Irrigantes do Canal Radicular/farmacologia , Cavidade Pulpar
7.
J Endod ; 49(2): 198-204, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36509168

RESUMO

INTRODUCTION: The purpose of this study was to evaluate the antimicrobial efficacy of a novel irrigation strategy using synchronized microbubble photodynamic activation (SYMPA) in a minimally prepared single canal. METHODS: Single-canal mandibular incisors were inoculated with Enterococcus faecalis for 3 weeks and randomly allocated to 4 groups based on the irrigation protocols: (1) control (saline), (2) conventional needle irrigation (CI), (3) ultrasonic-assisted irrigation (UI), and (4) irrigation with SYMPA. The first 3 groups were instrumented to size 25.07v (WaveOne Gold Primary; Dentsply Sirona, Johnson City, TN), and the SYMPA group was minimally prepared to size 20.07v (WaveOne Gold Small, Dentsply Sirona). The apical 5 mm was resected for microbiological assessment using the culture technique (colony-forming unit), adenosine-5'-triphosphate-based viability assay (relative luminescence units), and the percentage of live bacteria using confocal laser scanning microscopy. RESULTS: Log colony-forming units from the UI (2.37 ± 0.66) and SYMPA (2.21 ± 0.86) groups showed a reduction compared with the control (5.16 ± 0.75) and CI (4.08 ± 1.19) groups. Relative luminescence unit reduction was significant for UI (619.08 ± 352.78) and SYMPA (415.25 ± 329.51) compared with the control (1213.2 ± 880.03) (P < .05). The percentage of live bacteria was significantly lower in the UI and SYMPA groups compared with the control and CI groups. Although higher microbial reduction was observed in SYMPA compared with UI, there was no statistical significance (P > .05). CONCLUSION: SYMPA in minimally prepared canals showed significant antimicrobial efficacy. The novel irrigation strategy using SYMPA could be an effective disinfection strategy for minimally prepared root canals.


Assuntos
Anti-Infecciosos , Cavidade Pulpar , Cavidade Pulpar/microbiologia , Enterococcus faecalis/fisiologia , Microbolhas , Irrigantes do Canal Radicular/farmacologia , Irrigantes do Canal Radicular/uso terapêutico , Preparo de Canal Radicular/métodos , Hipoclorito de Sódio
8.
Int Endod J ; 56(1): 118-127, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36148855

RESUMO

AIM: Little is known about the influence of isthmus morphology on the debridement efficacy of activated irrigation. The aim of this study was to investigate the influence of isthmus morphology on the debridement efficacy of laser-activated irrigation (LAI), EDDY and needle irrigation (NI), and to explain the methods of isthmus cleaning by LAI and EDDY. METHODOLOGY: Four root canal models (apical diameter: 0.30 mm, taper: 0.06, curvature: 23°, length: 20 mm) were produced by CAD-CAM with different isthmus morphologies: long-wide (4 mm; 0.4 mm), long-narrow (4 mm; 0.15 mm), short-wide (2 mm; 0.4 mm) and short-narrow (2 mm; 0.15 mm). The isthmuses were filled with a hydrogel containing dentine debris. The canals were filled with irrigant and models were assigned to the following irrigation protocols (n = 240): needle irrigation (NI) with a 30G needle, Eddy, and LAI (2940 nm Er:YAG-laser, 15 Hz, 40 mJ, SWEEPS, tip at the canal entrance). Standardized images of the isthmuses were taken before and after irrigation, and the amount of removed hydrogel was determined using image analysis software and compared across groups using Kruskal-Wallis test followed by Dunn's multiple comparison. Visualization of the isthmus during activation was achieved using a high-speed camera. The pattern and speed of the flow in the isthmus as well as transient and stable cavitation were analysed using imaging software. RESULTS: Laser-activated irrigation, EDDY and NI removed more hydrogel in short-wide isthmuses than in narrow isthmuses (p < .001). LAI and EDDY removed more hydrogel than NI in every isthmus configuration (p < .001). EDDY showed eddies and stable cavitation, and LAI showed transient cavitation at each pulse, and pulsed horizontal flow with the highest particle speed in closed short isthmuses. CONCLUSIONS: Isthmus morphology influences debridement in all irrigation groups. Short-wide isthmuses were the easiest to clean while narrow isthmuses were the most challenging to clean. Width seems to be a more critical anatomical parameter than length. LAI and EDDY resulted in the greatest biofilm removal and performed better than NI. EDDY produced eddies and stable cavitation in the isthmus, and LAI showed transient cavitation and pulsed horizontal flow.


Assuntos
Cavidade Pulpar , Hidrogéis , Irrigantes do Canal Radicular , Lasers de Estado Sólido , Desbridamento , Tratamento do Canal Radicular
9.
J Endod ; 49(2): 205-211, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36528174

RESUMO

INTRODUCTION: Recurrent endodontic infections are primarily caused by Enterococcus faecalis and are more challenging to treat, compared with primary infection of the root canal system. Calcium hydroxide (CH) is used as an interappointment dressing in endodontics despite its inefficacy against E. faecalis and other pathogens. To improve antimicrobial properties and limit cytotoxicity of CH, we added salicylic acid to CH (CASA) to disinfect the canal. CASA overcomes the main pathogen responsible for recurrent endodontic infections. The aim of this study was to evaluate the antimicrobial activity of CASA and its cytotoxicity against dental pulp stem cells (DPSCs) and its effect on the differentiation potential of DPSCs. METHODS: Mature E. faecalis biofilm cultured on dentin chips was exposed to CASA and studied using confocal laser scanning microscopy. The dose-dependency of CASA was also studied using the liquid suspension test. The cytotoxicity was tested against DPSCs, and its effect on the expression of osteocalcin and alkaline phosphatase was studied. RESULTS: CASA produced larger zones of inhibition than CH for all species tested and demonstrated superior efficacy than CH against E. faecalis biofilm. Cytotoxicity studies indicated DPSC's high tolerance for CASA; addition of CASA to DPSCs was observed to increase the expression of biological markers related to mineralization. CONCLUSIONS: CASA was proved to have superior antibacterial efficacy against E. faecalis when compared with CH. It also increased the expression of some DPSC differentiation markers involved in mineralization.


Assuntos
Cavidade Pulpar , Endodontia , Cavidade Pulpar/microbiologia , Tratamento do Canal Radicular , Antibacterianos/farmacologia , Hidróxido de Cálcio/farmacologia , Enterococcus faecalis , Biofilmes , Irrigantes do Canal Radicular/farmacologia , Dentina
10.
Braz Oral Res ; 36: e068, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36507755

RESUMO

We aimed to evaluate the penetration of endodontic cement following the removal of calcium hydroxide (Ca(OH)2) dressing using the XP-endo Finisher in association with different irrigating solutions. Sixty premolars were instrumented and applied with a Ca(OH)2 dressing. To remove Ca(OH)2, the teeth were divided into six groups, each with a different volume of sodium hypochlorite (NaOCl) and ethylenediaminetetraacetic acid (EDTA), as well as solution stirring time with XP-endo Finisher (0, 30, and 60 sec). Root canals were filled using the lateral condensation technique. Fluorescein dye was added to the cement for microscopic laser scanning analysis. In the generated images, linear measurements were taken in micrometers, and their averages were calculated. To analyze the perimeter penetration ratio of the cement, the total perimeter of the canal and the segment of the total perimeter of the canal where the endodontic cement penetrated into the dentinal tubules were measured in micrometers. We found that using an XP-endo Finisher in irrigation was more effective than using a needle and syringe during the extension and penetration of endodontic cement. Shaking with XP-endo Finisher with 17% EDTA increased the extent and perimeter of the penetration of the endodontic cement into the dentinal tubules. However, using the XP-endo Finisher with EDTA only was more efficient than using the instrument interchangeably in NaOCl and EDTA. Although XP-endo Finisher contributes to the removal of Ca(OH)2, none of the protocols or instruments used removed all Ca(OH)2 from the root system.


Assuntos
Hidróxido de Cálcio , Hipoclorito de Sódio , Hidróxido de Cálcio/uso terapêutico , Ácido Edético/uso terapêutico , Hipoclorito de Sódio/uso terapêutico , Cimentos de Ionômeros de Vidro , Preparo de Canal Radicular , Cavidade Pulpar , Irrigantes do Canal Radicular/uso terapêutico
11.
BMC Oral Health ; 22(1): 601, 2022 12 13.
Artigo em Inglês | MEDLINE | ID: mdl-36514017

RESUMO

BACKGROUND: To examine post-operative pain (PP) after conventional irrigation and sonic activation methods, with and without laser disinfection in mandibular molars. METHODS: Eighty patients with symptomatic apical periodontitis were included in this randomized clinical study. There were four study groups. In group 1, conventional irrigation only was applied. In group 2, a sonic irrigation activation system (EDDY (VDW, Munich, Germany)), was applied. In groups 3 and 4, irradiation with a 980-nm diode laser was performed, following irrigation with the conventional method and sonic irrigation activation system, respectively. The patients were instructed to record their PP and analgesic intake using a numerical rating scale 8, 24, 48 h and 7 days post-procedure. A chi-square test, Fisher's exact chi-square test and Fisher-Freeman-Halton exact test were used to assess qualitative data. Inter-group and intra-group parameters were assessed using the Kruskal-Wallis test and Wilcoxon's test at a significance level of p < 0.05. RESULTS: There was no statistically significant difference among the groups in terms of age, sex, pre-operative pain, PP and analgesic intake (p > 0.05). CONCLUSIONS: The use of sonic irrigation activation system in the final irrigation protocol and irradiation with the 980-nm diode laser did not significantly reduce PP levels and analgesic intake.


Assuntos
Irrigantes do Canal Radicular , Preparo de Canal Radicular , Humanos , Preparo de Canal Radicular/métodos , Desinfecção , Irrigação Terapêutica/métodos , Dente Molar/cirurgia , Dor Pós-Operatória/prevenção & controle , Cavidade Pulpar
12.
BMC Oral Health ; 22(1): 660, 2022 12 31.
Artigo em Inglês | MEDLINE | ID: mdl-36585632

RESUMO

INTRODUCTION: Enterococcus faecalis (E. faecalis) is the most commonly isolated bacterium from infected root canals. It is found in the form of a biofilm, which makes it more resistant to antimicrobials, and requires optimal chemomechanical strategies to maximize root canal disinfection. AIM: To evaluate the efficacy of 4 different endodontic file systems against E. faecalis biofilm growth in root canals using colony-forming units per milliliter (CFU/mL) and scanning electron microscope (SEM). METHODS: Eighty-five extracted human mandibular premolars with straight root canals and apical diameters not larger than the #15 K-file were randomly selected. After performing a pilot study (n = 15) to determine the ideal incubation period for E. faecalis biofilm development, sixty-five root canals were infected with E. faecalis, incubated for 3 weeks, and then mechanically prepared using one of four single files (XP-endo Shaper, Hyflex EDM, One Curve, and Fanta. AFTM F One) (n = 15). Five infected root canals were excluded for the positive control. Five non-contaminated root canals were included for the negative control. Samples were collected using sterile paper points pre- and post-instrumentation to determine the bacterial load (CFU/mL). Root canals from each group were topographically evaluated at the coronal, middle, and apical segments using scanning electron microscope (SEM). Bacterial reduction data were estimated and statistically analyzed by Kruskal-Wallis and Mann-Whitney U tests (post hoc test) (P ≤ .05). RESULTS: XP-endo Shaper, Hyflex DEM, and One Curve significantly could eradicate E. faecalis biofilms in infected root canals with no significant difference among them compared to Fanta. AF™ F One. CONCLUSION: None of the systems were capable of completely eliminating biofilms. XP-endo Shaper, Hyflex EDM, and One Curve mechanically eliminated E. faecalis biofilms compared to Fanta. AF™ F One from infected root canals.


Assuntos
Cavidade Pulpar , Preparo de Canal Radicular , Humanos , Biofilmes , Cavidade Pulpar/microbiologia , Enterococcus faecalis , Projetos Piloto , Irrigantes do Canal Radicular , Tratamento do Canal Radicular
13.
Evid Based Dent ; 23(4): 148-149, 2022 12.
Artigo em Inglês | MEDLINE | ID: mdl-36526839

RESUMO

Study population and design This randomised observational study compared the analgesic efficacy of intra-canal local ibuprofen paste (Odontocide) with systemic ibuprofen capsules. Ninety patients with symptomatic apical periodontitis scheduled to undergo endodontic therapy were randomised to receive either local ibuprofen, systemic ibuprofen, or no ibuprofen (control). Using a visual analogue scale, pain scores were measured at set time points in the first 48 hours after endodontic instrumentation. The endodontic instrumentation procedures were standardised to use the same endodontic files, sodium hypochlorite, and EDTA irrigants. Canals were dressed with either Odontocide (local ibuprofen paste) or calcium oxide in the systemic ibuprofen and control groups.Data analysis Friedman and Wilcoxon tests were used to compare groups. A power calculation was listed in the methods of the paper which is an important and appropriate step.Results Systemic ibuprofen gave statistically significant better pain relief compared with local ibuprofen paste applied to root canals but only in the first 24 hours after root canal instrumentation. After the 24-hour mark, the systemic ibuprofen had a lower mean pain score but this was not statistically significant. Systemic ibuprofen was always statistically better at providing pain relief than the control.


Assuntos
Ibuprofeno , Periodontite Periapical , Humanos , Ibuprofeno/uso terapêutico , Irrigantes do Canal Radicular/uso terapêutico , Preparo de Canal Radicular/métodos , Dor Pós-Operatória , Periodontite Periapical/terapia , Ensaios Clínicos Controlados Aleatórios como Assunto , Estudos Observacionais como Assunto
14.
J Ayub Med Coll Abbottabad ; 34(4): 766-770, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36566396

RESUMO

BACKGROUND: The treatment of infected root canals is one of the most essential areas in dentistry. The endodontic therapy comprises of a sequence of steps that includes the purging of disease and infectious tissues from the root canal system. The objective of the study is to compare the effect of an experimental herbal root canal irrigant (Sapindus mukorossi) and Ethylenediaminetetraacetic acid, on the micro hardness of human dentin. METHODS: Ninety single rooted teeth were collected displayed intact external morphology the selected teeth were than divided randomly into 3 groups (n=30); 1 control and 2 experimental groups: Group A consisted of specimen treated with Ethanolic extract of Sapindus mukorossi. Group B consisted of specimen treated with 17% Ethylenediaminetetraacetic acid and Group C (control group) consisted of specimen of roots were sectioned immersed in distilled water. After the removal of crowns, the roots were sectioned embedded in the polymer resin leaving the root dentine exposed, the micro hardness was determined. Then, the samples from each group were treated with respective irrigant solution for 15 minutes and then observation regarding the micro hardness were noted again by using Vickers micro hardness tester. Analysis of data were obtained by application of Wilcoxon signed rank test. RESULTS: The study revealed that there was no significant difference noted in dentin microhardness of group "A" sample after dipping in experimental irrigant solution (Sapindus mukorossi). However, the study found significant difference in context of decrease in dentin microhardness after dipping a sample in a group "B" irrigant solution (17% Ethylenediaminetetraacetic acid). CONCLUSIONS: In order to preserve the strength of dentin that will ultimately be beneficial for increase in survival of teeth in function, probably the herbal extract of Sapindus mukorossi would be better candidate for future endeavour and presented as an economic and effective alternative amongst root canal irrigants.


Assuntos
Irrigantes do Canal Radicular , Sapindus , Humanos , Cavidade Pulpar , Dentina , Ácido Edético/farmacologia , Irrigantes do Canal Radicular/farmacologia , Hipoclorito de Sódio/farmacologia
15.
Lasers Med Sci ; 38(1): 8, 2022 Dec 21.
Artigo em Inglês | MEDLINE | ID: mdl-36539627

RESUMO

This study aimed to assess the apical extrusion of sodium hypochlorite in immature teeth caused by needle irrigation, ultrasonic irrigation, EDDY, Er:YAG, and diode lasers. Seventy-five human maxillary anterior teeth were selected. After the apical 3 mm of the roots was resected, the canal lumen was enlarged with #2 to #5 Gates-Glidden burs. The teeth were fixed to the lid of a flat-sided clear plastic container, and the plastic containers were filled with 0.2% agarose gel containing 1 mL of 0.1% m-cresol purple. Then, the specimens were allocated into five groups according to the irrigation activation techniques: needle irrigation, ultrasonic irrigation, EDDY, Er:YAG laser, and diode laser. The apical extrusion of sodium hypochlorite caused the color change in the gel. The containers were digitally photographed, and the percentage of pixels of irrigant extrusion (the color change) in each photograph was calculated. The diode laser group showed a significantly lower percentage of pixels than needle irrigation, EDDY, and Er:YAG laser (p < 0.05). The irrigant extrusion value of the ultrasonic irrigation group was significantly lower than that of the EDDY and Er:YAG laser groups (p < 0.05). There was no significant difference between the other groups (p > 0.05). All the groups caused irrigant extrusion in the immature teeth. The diode laser and ultrasonic irrigation caused less sodium hypochlorite extrusion than EDDY and Er:YAG laser. The needle irrigation showed more irrigant extrusion than the only diode laser group.


Assuntos
Lasers de Estado Sólido , Hipoclorito de Sódio , Humanos , Preparo de Canal Radicular , Lasers Semicondutores/uso terapêutico , Ultrassom , Irrigantes do Canal Radicular , Lasers de Estado Sólido/uso terapêutico , Plásticos , Irrigação Terapêutica , Cavidade Pulpar
16.
BMC Oral Health ; 22(1): 590, 2022 12 09.
Artigo em Inglês | MEDLINE | ID: mdl-36494809

RESUMO

The purpose of this study is to investigate the effects of different root canal irrigation protocols applied to the dentin and artificial aging procedures on the micro pushout bond strength (mPBS) between dentin and hybrid ceramic posts. Seventy-five single-rooted mandibular premolar teeth were divided into 5 groups (Gr1-5). 50 of the teeth were used for the mPBS tests (n = 10), whereas 25 were used for the smear layer examinations (n = 5). Post space were prepared and irrigated with different irrigation-protocols in each group. (Gr1:[SS], Gr2:[NaOCl] + SS, Gr3:[EDTA] + NaOCl + SS, Gr4:[MA] + NaOCl + SS, Gr5:[Ch] + NaOCl + SS). Post and core pattern were fabricated with pattern resin and a fiber post, after scanning, the posts were milled with Vita Enamic resin ceramic block, and cemented. After 7 days the roots were sliced at thicknesses of 1 mm; half of them were subjected to mPBS test, while the other half were tested after undergoing mechanical cycling for artificial aging. For data analysis, the Shapiro-Wilk test was utilized to test normal distributions, 3-way analysis of variance was used to compare mPBS, and Tukey's HSD test was conducted for multiple comparisons. SEM analysis was performed for examination of failure modes and smear layer removal. Different root canal irrigation protocols affected mPBS significantly. While Gr4 had the highest mPBS, Gr1 had the lowest. Regarding to different zones, the highest mPBS was in coronal zone, and the lowest one was in the apical zone. The aging procedure also led to a statistically-significant decrease in mPBS. Most frequent failure modes were cohesive failure in dentin and mixed failure. Irrigation with 7%MA (Gr4) showed better performance than 17% EDTA (Gr3) in smear layer removal, especially at the apical zone of the tooth. This is critical for the success of root canal treatment and increased the mPBS to a higher extent in all zones of the tooth.


Assuntos
Colagem Dentária , Camada de Esfregaço , Humanos , Irrigantes do Canal Radicular/uso terapêutico , Irrigantes do Canal Radicular/química , Cavidade Pulpar , Dentina , Colagem Dentária/métodos , Hipoclorito de Sódio/uso terapêutico , Hipoclorito de Sódio/química , Ácido Edético/análise , Ácido Edético/química , Ácido Edético/farmacologia , Dente Pré-Molar , Preparo de Canal Radicular/métodos , Teste de Materiais
17.
BMC Oral Health ; 22(1): 643, 2022 12 26.
Artigo em Inglês | MEDLINE | ID: mdl-36567353

RESUMO

Dental pulp and periapical diseases are common conditions in stomatology, caused by various pathogenic microorganisms. Antimicrobial peptides, as new antibiotics, offer promising applications in the irrigation and disinfection medicaments for root canals.One patient with chronic periapical periodontitis was selected to extract the clinical pathogenic bacteria. Porphyromonas gingivalis (Pg) (ATCC 33,277), Streptococcus mutans (Sm) (ATCC 25,175), and Prevotella intermedius (Pi) (ATCC 25,611) were used as test strains. The effects of plantaricin (Pln) 149 on the biofilm formation and growth in infected root canals were evaluated by RT-PCR, laser confocal scanning microscopy, and bacterial diversity analysis. In addition, the cytotoxicity of Pln 149 (100 µg/mL) to human dental pulp stem cells (hDPSCs) was assessed using an MTT assay. Pln 149 exhibited significant inhibitory effects on Pg Sm and Pi (P < 0.05), with significant differences in the biofilm images of the laser confocal scanning microscope (P < 0.05). There were no significant differences in hDPSCs viability or proliferation between the Pln 149 and control groups. Considering the excellent antimicrobial effects and low cytotoxicity, we suggest that Pln 149 might be a promising option for root canal irrigation solutions.


Assuntos
Antibacterianos , Bacteriocinas , Cavidade Pulpar , Irrigantes do Canal Radicular , Preparo de Canal Radicular , Humanos , Antibacterianos/farmacologia , Cavidade Pulpar/microbiologia , Irrigantes do Canal Radicular/química , Irrigantes do Canal Radicular/farmacologia , Hipoclorito de Sódio/farmacologia , Bacteriocinas/farmacologia , Porphyromonas gingivalis/efeitos dos fármacos , Porphyromonas gingivalis/isolamento & purificação , Streptococcus mutans/efeitos dos fármacos , Streptococcus mutans/isolamento & purificação , Prevotella/efeitos dos fármacos , Prevotella/isolamento & purificação
18.
Braz. j. oral sci ; 21: e224013, jan.-dez. 2022. ilus
Artigo em Inglês | LILACS, BBO - Odontologia | ID: biblio-1354702

RESUMO

Aim: The aim of the study was to evaluate the cleaning of mandibular incisors with WaveOne Gold® (WO) under different preparation techniques. Methods: A total of 210 human mandibular incisors were selected and divided into seven groups (n = 30), prepared by WO single-files (Small 20/.07 ­ WOS; Primary 25/.07 ­ WOP; Medium 35/.06 ­ WOM; or Large 45/.05 - WOL) and sequential-file techniques (WOS to WOP; WOS to WOM; and WOS to WOL). Further subdivision was made according to irrigation protocol: control group (manual irrigation - CON), E1 Irrisonic® - EIR, and EDDY® - EDD. Debris removal and the smear layer were evaluated by scanning electron microscopy. Data were analyzed by using Spearman's correlation test. The significance level was set at 5%. Results: For debris and smear layer removal, WOS and WOP, EIR differed from CON and EDD (p <0.05). Conclusion: Regardless of the instrumentation used, the agitation of the irrigant solution provided better cleanability. These findings reinforce the need for agitation techniques as adjuvants in cleaning root canal systems in mandibular incisors


Assuntos
Irrigantes do Canal Radicular , Microscopia Eletrônica de Varredura , Preparo de Canal Radicular , Endodontia
19.
Niger J Clin Pract ; 25(11): 1831-1837, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36412290

RESUMO

Background: Gravity impact has been mainly evaluated in mature teeth related to debris extrusion, even though it may affect the amount of apically extruded irrigant. In the literature the influence of gravity on the amount of apically extruded irrigant in immature teeth has been studied by a 45o inclined plate to mimic the position of the maxillary teeth and 90o for the mandibular teeth. However, patients are positioned horizontally in the dental chair while treatment. There is no study in the literature testing the horizontal position to mimic the clinical settings realistically. Aim: The aim of this study was to evaluate the influence of various irrigation systems on the amount of extruded irrigant in simulated immature maxillary and mandibular teeth irrigated in vertical and horizontal positions. Materials and Methods: Twenty-five maxillary central incisors with an apical opening of 1.3 mm in diameter were included. Irrigation procedures were performed with EndoVac, closed-ended, and open-ended needles using a VATEA peristaltic pump. The amount of apically extruded irrigant was determined using a microbalance. Statistical analysis was performed using the Kruskal-Wallis test. Results: The EndoVac system caused almost no irrigant extrusion in all tested positions (P > 0.05); however, closed-ended and open-ended needles extruded more irrigant in a mandibular vertical position compared to maxillary vertical (P < 0.05) and maxillary horizontal positions (P < 0.05). Open-ended needles extruded the highest amount of irrigant. Conclusions: The EndoVac macrocannula is a more reliable and safer irrigation system as it prevents irrigant extrusion independent of the position of the tooth.


Assuntos
Irrigantes do Canal Radicular , Preparo de Canal Radicular , Humanos , Preparo de Canal Radicular/métodos , Ápice Dentário , Agulhas
20.
Future Microbiol ; 17: 1487-1499, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36321479

RESUMO

To enhance endodontics, the search for new antibacterials that can improve infected tooth root canal treatment is ongoing. As potent antibacterial and antibiofilm agents, essential oils (EOs) have been suggested as novel endodontic materials. Several studies indicate that EO-based irrigants and medicaments show promising reductive potential against the most important intracanal pathogen, Enterococcus faecalis, and notably contribute to intracanal biofilm eradication. In terms of additional benefits that EO-based endodontic materials can provide, their antioxidant and anti-inflammatory potential are also important, but they have only scarcely been explored in research. Investigations into the benefits of EO-based endodontic materials together with their biocompatibility are needed. The results presented in this review strongly encourage further research on this topic.


Assuntos
Óleos Voláteis , Irrigantes do Canal Radicular , Óleos Voláteis/farmacologia , Óleos Voláteis/uso terapêutico , Enterococcus faecalis , Biofilmes , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico
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