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1.
Food Res Int ; 159: 111620, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-35940811

RESUMO

This study aimed to assess Spirulina platensis, Chlorella vulgaris, Scenedesmus quadricauda, and Lagerheimia longiseta microalgae potential as protective agents for probiotic cultures [(Lactobacillus acidophilus (La-05) and Lacticaseibacillus casei (Lc-01)] during freeze-drying, refrigeration storage (4 °C, 120 days), and in vitro simulated gastrointestinal conditions (SGIC). The occurrence of membrane damage and ultrastructural aspects of the cells were also verified. Fructooligosaccharides (FOS) were used as a positive control and saline solution as a negative control. The effects of the cryoprotectants on probiotic survival depended on the tested probiotic culture and microalgae biomass. For La-05, all tested cryoprotectants caused a lower reduction in probiotic counts during the freeze-drying and up to 90 days of storage. S. platensis kept higher probiotic counts during storage, while C. vulgaris protected the probiotic against the SGIC. L. longiseta decreased the probiotic membrane damage, mainly due to the production of exopolysaccharides, which was observed in the scanning electron microscopy (SEM). For Lc-01, all tested cryoprotectants promoted a lower reduction in probiotic counts up to 120 days of storage. FOS and S. quadricauda protected the probiotics during freeze-drying and refrigeration storage, while C. vulgaris protected the probiotic against the SGIC and caused lower membrane damage, mainly due to physical protection observed in SEM. In conclusion, microalgae biomasses exerted similar or better cryoprotectant effects on probiotics than FOS, a recognized cryoprotective agent.


Assuntos
Chlorella vulgaris , Lactobacillus casei , Microalgas , Probióticos , Biomassa , Crioprotetores/farmacologia , Água Doce , Lactobacillus acidophilus , Probióticos/química
2.
Sci Rep ; 12(1): 13401, 2022 Aug 04.
Artigo em Inglês | MEDLINE | ID: mdl-35927320

RESUMO

The current study aimed to figure out the effect of using a combination of 2% inulin, and 2% Fructo-oligosaccharides (FOS) with Lactobacillus acidophilus and their bacteriocin on some yogurt properties such as coagulation time, extending the shelf life of set yogurt and its microbiological quality, also the acceptance by consumers. The results indicated that coagulation time increased by 22.75% in yogurts prepared with Lactobacillus acidophilus and their bacteriocins compared to the control, and titratable acidity increased gradually in all treatments during storage. Hence control acidity (%) increased from 0.84 ± 0.02A at zero time to 1.23 ± 0.03A after 14 days of cold storage, while treatment (T4) was 0.72 ± 0.01C at zero time and reached 1.20 ± 0.5A after 39 days at the same conditions. The sensory properties showed the superiority of inulin, FOS, and Lactobacillus acidophilus bacteriocin groups. Lactobacillus bulgaricus, Streptococcus thermophiles, and Lactobacillus acidophilus count increased in the treatments compared to the control group, with an extended shelf life to 39 days of storage in the medicines containing lactobacillus acidophilus bacteriocin. Coliforms, Moulds, and yeasts did not detect in the treatments comprising 2% inulin, 2% FOS, and lactobacillus acidophilus bacteriocin for 39 days of refrigerated storage. This study proved that 2% inulin, 2% FOS, and Lactobacillus acidophilus bacteriocin fortification extended the shelf life by more than 5 weeks.


Assuntos
Bacteriocinas , Probióticos , Bacteriocinas/farmacologia , Inulina/farmacologia , Lactobacillus acidophilus , Oligossacarídeos/farmacologia , Probióticos/farmacologia , Iogurte/microbiologia
3.
Food Funct ; 13(15): 8107-8118, 2022 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-35793105

RESUMO

Lactobacillus acidophilus, as the main probiotic in the small intestine, has a role in modulating intestinal immune activity; however, its potential molecular regulatory mechanisms have rarely been investigated. To investigate the effects of Lactobacillus acidophilus LA85 in immunoprophylaxis, LA85 at the dose of 1 × 108 CFU mL-1, 1 × 109 CFU mL-1, and 1 × 1010 CFU mL-1 were orally administered to immunocompromised ICR mice that had been treated intraperitoneally with 80 mg kg-1 cyclophosphamide. The experimental results showed that LA85 could regulate the Notch signaling pathway and promote intestinal mucosal repair. And it could enhance the immune response of the body through the TLR4/NF-κB signaling pathway. 16S rDNA gene sequencing revealed that LA85 reduced the ratio of Firmicutes/Bacteroidetes and reshaped the gut microbiota. Furthermore, we discovered the correlation between Erysipelatoclostridium, Rikenella, and the intestinal Notch signaling pathway, and further discovered the potential mechanism of LA85 in intestinal mucosal repair. Based on the results of the study, we found that the effect of high doses of LA85 was more significant. Therefore, LA85 can be used as a dietary supplement to potentially enhance the immune capacity of patients.


Assuntos
Microbioma Gastrointestinal , Probióticos , Animais , Ciclofosfamida/farmacologia , Terapia de Imunossupressão , Lactobacillus acidophilus/metabolismo , Camundongos , Camundongos Endogâmicos ICR , NF-kappa B/genética , NF-kappa B/metabolismo , Probióticos/farmacologia , Transdução de Sinais , Receptor 4 Toll-Like/genética
4.
Immunohematology ; 38(2): 55-61, 2022 Jul 05.
Artigo em Inglês | MEDLINE | ID: mdl-35852063

RESUMO

The use of probiotics brings numerous benefits to the immune system, including an increase in antibody production. The development of ABO antibodies may occur naturally due to the bacteria of the intestinal microbiota. However, high titers of ABO antibodies can lead to hemolytic disease of the fetus and newborn and can cause immune transfusion reactions. In this context, this study aimed to evaluate the effect of probiotic consumption on ABO antibody titers in humans. ABO blood group, ABO antibody titer, and fecal pH and Bifidobacteria concentration were determined for 126 healthy individuals before and after daily consumption of yogurt containing Lactobacillus acidophilus and Bifidobacterium lactis over a 1-month period. No changes in fecal pH were observed after probiotic consumption, regardless of ABO blood group. There was, however, an increase in the fecal concentration of Bifidobacteria in individuals with blood group A but not for those with group B or O. A decrease in the titer of anti-B was observed, despite the increase in the concentration of Bifidobacteria in feces being unrelated to fecal pH, in blood group A individuals. Our study, therefore, sought to understand the relationship between probiotics and the antibody titer of the ABO blood system. Despite our findings, further human studies are needed with other probiotic strains and molecular analyses of the intestinal microbiota.


Assuntos
Sistema ABO de Grupos Sanguíneos , Probióticos , Bifidobacterium , Humanos , Recém-Nascido , Lactobacillus acidophilus , Iogurte/microbiologia
5.
Nutrients ; 14(13)2022 Jun 24.
Artigo em Inglês | MEDLINE | ID: mdl-35807801

RESUMO

Investigations that focused on the protective role of probiotics against Surgical Site Infections (SSI) in multiple-trauma (MT) patients are generally few, probably due to the complexity of the concept of trauma. We aimed to assess the efficacy of a four-probiotic regime to reduce the incidence of SSI in MT patients, with a brain injury included. MT patients, being intubated and expected to require mechanical ventilation for >10 days, were randomly allocated into placebo (n = 50) or probiotic treatment (n = 53) comprising Lactobacillus acidophilus LA-5 (1.75 × 109 cfu), Lactiplantibacillus plantarum UBLP-40 (0.5 × 109 cfu), Bifidobacterium animalis subsp. lactis BB-12 (1.75 × 109 cfu), and Saccharomycesboulardii Unique-28 (1.5 × 109 cfu) in sachets. All patients received two sachets of placebo or probiotics twice/day for 15 days and were followed-up for 30 days. The operations were classified as neurosurgical, thoracostomies, laparotomies, orthopedics, and others; then, the SSI and the isolated pathogen were registered. A total of 23 (46.0%) and 13 (24.5%) infectious insults in 89 (50 placebo patients) and 88 (53 probiotics-treated) operations (p = 0.022) were recorded, the majority of them relating to osteosynthesis-17 and 8, respectively. The most commonly identified pathogens were Staphylococcus aureus and Acinetobacter baumannii. Our results support published evidence that the prophylactic administration of probiotics in MT patients exerts a positive effect on the incidence of SSI.


Assuntos
Bifidobacterium animalis , Probióticos , Bifidobacterium , Método Duplo-Cego , Humanos , Lactobacillus acidophilus , Probióticos/uso terapêutico , Infecção da Ferida Cirúrgica/prevenção & controle
6.
Nutrients ; 14(13)2022 Jun 28.
Artigo em Inglês | MEDLINE | ID: mdl-35807868

RESUMO

The evaluation of probiotics' efficacy in treating irritable bowel syndrome is supported by an increasing number of clinical studies based on a heterogeneous approach of products tested and the patient cohort involved. Although the role of gut microbiota dysbiosis in IBS pathogenesis and the beneficial contribution of probiotics were demonstrated, a tool to discriminate symptom-specific strains and a personalised medicine protocol are still lacking. Thus, this study employs, for the first time, a method that combines the preferred reporting items for systematic reviews and meta-analysis and multi-criteria decision analysis methods in a structured decision-making tool to analyze the efficacy of probiotic mix, in order to identify the most effective formulation and to discriminate which probiotics are more efficient in treating different symptoms. The PRISMA methodology resulted in a qualitative and quantitative analysis of 104 clinical studies from 2011 to 2021, revealing a prevalence of Lactobacillus rhamnosus, Lactobacillus acidophilus, and Bifidobacterium animalis subsp. lactis. MCDA analysis showed that formulations based on Lactobacillus rhamnosus and Lactobacillus acidophilus have the highest efficacy, especially on quality of life, bloating, and abdominal pain. This methodological approach could become more specific by modelling clinical studies according to the age and gender of patients and probiotic strain.


Assuntos
Bifidobacterium animalis , Síndrome do Intestino Irritável , Lactobacillus rhamnosus , Probióticos , Técnicas de Apoio para a Decisão , Humanos , Síndrome do Intestino Irritável/tratamento farmacológico , Lactobacillus acidophilus , Probióticos/uso terapêutico , Qualidade de Vida
7.
Arch Biochem Biophys ; 727: 109327, 2022 09 30.
Artigo em Inglês | MEDLINE | ID: mdl-35760145

RESUMO

This study synthesized new modified imaging nanocapsules (NCs) of gallium@deferoxamine/folic acid/chitosan/polyaniline/polyvinyl alcohol (Ga@DFA/FA/CS/PANI/PVA) containing Morus nigra extract by selenium nanoparticles prepared from Lactobacillus acidophilus. Se nanoparticles were then deposited on (Ga@DFA/FA/CS/PANI/PVA) using the impregnation method. The modified contrast agents were further mixed with M. nigra extract, and their antibacterial activities were investigated by applying them on L929 cell lines. The influence of variable factors including surfactant, solvent, aqueous phase, pH, buffer, minimum Inhibitory concentration (MIC), minimum bactericidal concentration (MBC), cytotoxicity on cancer cells., antibiotic, antibiogram, release and loading, stirring effect, the concentration of nanoparticle, olive oil, and thermotical methods was investigated. The structure and morphology of the synthesized contrast agents were characterized by zeta potential sizer analysis (ZPS), X-Ray diffraction (XRD), Fourier-transform infrared (FT-IR), energy dispersive X-ray (EDX), ultraviolet-visible (UV-Vis) spectra, and scanning electron microscope (SEM). The experimental section was conducted and monitored by response surface methods (RSM) and MTT, MIC, MBC, and cancer cytotoxic conversion assay. Antibiogram testing of NCs on Pseudomonas aeruginosa bacteria was successful, and MIC = 2 factor was obtained with less harmful effect.


Assuntos
Quitosana , Nanopartículas Metálicas , Nanocápsulas , Selênio , Antibacterianos/química , Antibacterianos/farmacologia , Meios de Contraste , Lactobacillus acidophilus , Nanopartículas Metálicas/química , Testes de Sensibilidade Microbiana , Extratos Vegetais/química , Selênio/química , Espectroscopia de Infravermelho com Transformada de Fourier , Difração de Raios X
8.
Food Chem ; 395: 133564, 2022 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-35763922

RESUMO

We herein delved into the microencapsulation of Lactobacillus acidophilus (LA) into solid lipid microparticles (SLMs) via the cryomilling technique. For this aim, a frozen lipid mixture containing LA was pulverized at different times (7, 14, 21, 28, and 35 min) using a cryogenic mixer mill to produce probiotic-loaded SLMs. The impacts of different cryomilling durations on the SLMs properties (morphology, particle size, water activity, polymorphism, crystallinity, and thermal behavior) and the viability of LA were evaluated. Microencapsulation improved the viability of LA in simulated gastrointestinal fluids, heat stress, and different concentrations of salt and sucrose. SLMs also were suitable to be incorporated into foods. However, once the cryomilling time was prolonged, the viability of encapsulated LA declined, and particle size grew. The cryomilling technique showed great potential as an alternative approach for encapsulation due to the lack of solvent, short processing time, and simplicity.


Assuntos
Lactobacillus acidophilus , Probióticos , Lipídeos , Tamanho da Partícula
9.
Eur J Pharm Sci ; 175: 106235, 2022 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-35697287

RESUMO

Olsalazine is a typical 5-aminosalicylic acid (5-ASA) drug that depends on gut microbiota to liberate its anti-inflammatory moiety 5-ASA in the treatment of ulcerative colitis (UC). In recent decades, 5-ASA drugs combined with probiotics have achieved a better effective treatment for UC. Mechanisms of combination therapy have been widely discussed from a pharmacodynamic perspective. However, it is still unclear whether the better therapeutic efficacy of combination therapy was made by changing the metabolism of 5-ASA drugs in the colon under the regulation of probiotics. In the present study, combined with pharmacokinetic and gut microbiota analyses, we systematically evaluated the potential effect of Lactobacillus acidophilus (L. acidophilus) on the metabolism of Olsalazine at three levels (pharmacokinetic characteristics, metabolic microbiota, and metabolic enzymes) to offer some insights into this issue. As pharmacokinetic results showed, L. acidophilus barely had an influence on the pharmacokinetic parameters of Olsalazine, 5-ASA, and N-Ac-5-ASA. Notably, the colonic exposure of 5-ASA was not affected by L. acidophilus. Gut microbiota results also illustrated that L. acidophilus did not change the total abundance of azoreductase (azoR) and N-acetyltransferase (NAT) associated gut microbiota and enzymes, which are involved in the metabolism of Olsalazine. Both pharmacokinetic and gut microbiota results revealed that L. acidophilus did not increase the colonic exposure of 5-ASA to improve the efficacy of combination therapy. L. acidophilus played its role in UC treatment by regulating gut microbiota composition and amino acid, phenolic acid, oligosaccharide, and peptidoglycan metabolic pathways. There was no potential medication risk of combination therapy of Olsalazine and L. acidophilus. In summary, this research provided strong evidence of medication safety and a comprehensive understanding of therapeutic advantages for combination therapy of probiotics and 5-ASA drugs from the pharmacokinetic and gut microbiota perspectives.


Assuntos
Colite Ulcerativa , Microbioma Gastrointestinal , Probióticos , Ácidos Aminossalicílicos , Animais , Colite Ulcerativa/tratamento farmacológico , Colite Ulcerativa/metabolismo , Lactobacillus acidophilus , Mesalamina/uso terapêutico , Ratos
10.
Front Cell Infect Microbiol ; 12: 863208, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35646732

RESUMO

The vaginal microbiota plays vital protection in women. This probiotic activity is caused not only by individual Lactobacillus species but also by its multi-microbial interaction. However, the probiotic activity promoted by multi-microbial consortia is still unknown. The aim of this study was the individual and collective analysis on the prevalence of five vaginal lactobacilli (Lactobacillus iners, Lactobacillus crispatus, Lactobacillus gasseri, Lactobacillus jensenii, and Lactobacillus acidophilus) among healthy women and women with bacterial vaginosis (BV) or aerobic vaginitis (AV). PCR assays were realized on 436 vaginal samples from a previous study. Chi-square, univariable, and multivariable logistic regression analyses with the Benjamini-Hochberg adjustment evaluated associations between these lactobacilli and vaginal microbiota. Multi-microbial clustering model was also realized through Ward's Minimum Variance Clustering Method with Euclidean squared distance for hierarchical clustering to determine the probiotic relationship between lactobacilli and vaginal dysbiosis. Concerning the individual effect, L. acidophilus, L. jensenii, and L. crispatus showed the highest normalized importance values against vaginal dysbiosis (100%, 79.3%, and 74.8%, respectively). However, only L. acidophilus and L. jensenii exhibited statistical values (p = 0.035 and p = 0.050, respectively). L. acidophilus showed a significant prevalence on healthy microbiota against both dysbioses (BV, p = 0.041; and AV, p = 0.045). L. jensenii only demonstrated significant protection against AV (p = 0.012). Finally, our results evidenced a strong multi-microbial consortium by L. iners, L. jensenii, L. gasseri, and L. acidophilus against AV (p = 0.020) and BV (p = 0.009), lacking protection in the absence of L. gasseri and L. acidophilus.


Assuntos
Vaginose Bacteriana , Vulvovaginite , Análise por Conglomerados , Disbiose , Equador , Feminino , Humanos , Lactobacillus , Lactobacillus acidophilus , Consórcios Microbianos , Vaginose Bacteriana/epidemiologia , Vaginose Bacteriana/microbiologia , Vaginose Bacteriana/prevenção & controle
11.
BMC Oral Health ; 22(1): 230, 2022 06 09.
Artigo em Inglês | MEDLINE | ID: mdl-35681128

RESUMO

BACKGROUND: Preventive measures are essential during the length of orthodontic treatment to reduce the risk of decalcification and white spot lesions formation. With the evolution of procedures that enable coating of the orthodontic brackets using nanoparticles known for their good antibacterial activity, coating the brackets with nanoparticles of silver, zinc oxide and combination of silver and zinc oxide to evaluate their antibacterial effect in comparison to a control group without coating was carried out in this study. METHODS: Four groups of 12 brackets each were included in the study. The coating procedure was carried out using physical vapor deposition. The antibacterial activity was tested on Streptococcus mutans and Lactobacillus Acidophilus using colony forming count. The antibacterial activity was evaluated immediately after coating and later after 3 months. RESULTS: Brackets coated with combination of silver and zinc oxide nanoparticles had the highest ability on reduction of both Streptococcus mutans and Lactobacillus Acidophilus count followed by silver nanoparticles and then zinc oxide nanoparticles. No significant difference was found between the first and second antibacterial tests. CONCLUSION: The silver/zinc oxide nanoparticles coated brackets had the highest antibacterial effect in comparison to silver nanoparticles and zinc oxide nanoparticles individually coated brackets on Streptococcus mutans and Lactobacillus acidophilus, and all types of coatings showed enhanced antibacterial effect in comparison to the uncoated bracket. Coating of orthodontic brackets could be further assessed in clinical application to prevent decalcification.


Assuntos
Nanopartículas Metálicas , Braquetes Ortodônticos , Óxido de Zinco , Antibacterianos/farmacologia , Humanos , Lactobacillus acidophilus , Prata/farmacologia , Streptococcus mutans , Zinco , Óxido de Zinco/farmacologia
12.
Food Chem Toxicol ; 166: 113244, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-35728727

RESUMO

Sour cream is a dairy product with good potential to act as a food carrier for probiotic bacteria. The effect of probiotic cultures on the quality of sour cream from dry dairy ingredients was assessed during 5 weeks of storage. The treatments design was as follows: Bifidobacterium, Bifidobacterium + Lactobacillus casei, Lactobacillus acidophilus, Lactobacillus acidophilus + Lactobacillus casei and Lactobacillus casei. Fatty acids profile, texture, microbial load and sensory characteristics were analyzed. Comparison of fatty acids of different creams produced showed that there is a significant difference between the produced creams. There are a wide range of fatty acids in the structure of sour cream fat. Also, with the addition of these bacteria, the texture of the samples became firmer during storage. The sensory analyses showed that the addition of all three bacteria increased the overall acceptance of this product by the panelists. It is suggested that sour cream fortified with probiotics well orientated functional food had accepted composition and sensory properties.


Assuntos
Lactobacillus casei , Probióticos , Bifidobacterium , Ácidos Graxos , Lactobacillus acidophilus
13.
BMC Microbiol ; 22(1): 167, 2022 06 27.
Artigo em Inglês | MEDLINE | ID: mdl-35761217

RESUMO

Usage of "probiotics" for treatment of food-borne pathogens associated diseases, makes a significant reduction in transmission of resistant bacteria, and antimicrobial resistance genes from aquaculture environments to humans. In this research, the authors aim to evaluate the immunomodulatory, and histological effects of two probiotic strains on the Zebrafish model. Fish models were treated with Lactobacillus delbrueckii (G2), Lactobacillus acidophilus (G3) and both probiotics (G4) and compared with the control group (G1) (only infected by pathogen and receiving no probiotic). Biometric tests, height, weight, and mortality rate of the fishes were assessed. Afterward, RT-PCR was conducted for bacterial existence of probiotic strains, and quantitative assessment of alterations in targeted immune genes. Subsequently, histological sampling was done for investigation of spatial distribution, and villus length in proximal, middle, and distal sections of intestinal tissues. Based on the results, G4 showed the highest gene expression for Lactobacillus acidophilus after 28 days (P < 0.05). G4 also showed an increase in the number of goblet cells and villus length in the middle and distal sections of intestinal tissue after 56 days. Furthermore, after 56 days, the highest number of intraepithelial cells was observed in the proximal sections of intestinal tissue in G4. G2 and G3 showed significant differences in comparison with G1 (P < 0.05). After 60 days, the highest gene expression for Lactobacillus bulgaricus was found in group treated with only this probiotic bacteria. The highest expression level of IL-1ß and TNF-α were found in G1. The highest survival rate was in the case of groups only treated with Lactobacillus bulgaricus (G2). To sum up, it seems that usage of probiotics for the improvement of public health and fisheries industries can be helpful.


Assuntos
Anti-Infecciosos , Lactobacillus delbrueckii , Probióticos , Aeromonas hydrophila , Animais , Lactobacillus acidophilus , Lactobacillus delbrueckii/genética , Probióticos/farmacologia , Peixe-Zebra/microbiologia
14.
Sci Rep ; 12(1): 9168, 2022 06 02.
Artigo em Inglês | MEDLINE | ID: mdl-35654932

RESUMO

We aimed to explore the lncRNA-miR-mRNA network in response to Lactobacillus acidophilus (L. acidophilus) consumption in rectal cancer patients. The candidate miRs were first taken from the GEO and TCGA databases. We constructed the lncRNA-miR-mRNA network using the high-throughput sequencing data. At last, we created a heatmap based on the experimental data to show the possible correlation of the selected targets. The expression levels of selected targets were measured in the samples of 107 rectal cancer patients undergoing placebo and probiotic consumption and 10 noncancerous subjects using Real-Time PCR. Our analysis revealed a group of differentially expressed 12 miRs and 11 lncRNAs, and 12 genes in rectal cancer patients. A significant expression increase of the selected tumor suppressor miRs, lncRNAs, and genes and a substantial expression decrease of the selected oncomiRs, onco-lncRNAs, and oncogenes were obtained after the probiotic consumption compared to the placebo group. There is a strong correlation between some network components, including miR-133b and IGF1 gene, miR-548ac and MSH2 gene, and miR-21 and SMAD4 gene. In rectal cancer patients, L. acidophilus consumption was associated with improved expression of the lncRNA-miR-mRNA network, which may provide novel monitoring and therapeutic approaches.


Assuntos
MicroRNAs , Probióticos , RNA Longo não Codificante , Neoplasias Retais , Redes Reguladoras de Genes , Humanos , Lactobacillus acidophilus/genética , Lactobacillus acidophilus/metabolismo , MicroRNAs/genética , MicroRNAs/metabolismo , Probióticos/uso terapêutico , RNA Longo não Codificante/genética , RNA Longo não Codificante/metabolismo , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Neoplasias Retais/genética
15.
Nutrients ; 14(10)2022 May 19.
Artigo em Inglês | MEDLINE | ID: mdl-35631269

RESUMO

In the past decades, the regulation of pro-inflammatory cytokine production, including interleukin-8 (IL-8), has been the goal of many targeted therapeutic interventions for Necrotising enterocolitis (NEC), a gastrointestinal disease commonly associated with a very low birth weight in preterm infants. In this study, the ability to regulate the production of IL-8 of the water-soluble non-starch polysaccharide (WS-NSP) from taro corm (Tc-WS-NSP) extracted using a conventional (CE) or improved conventional (ICE) extraction method, of the probiotics Lactobacillus acidophilus, Bifidobacterium breve, and Bifidobacterium infantis, and their synbiotic mixtures were evaluated. The TNF-α stimulated HT-29 cells were incubated with undigested or digested Tc-WS-NSPs (CE or ICE), probiotics, and their synbiotic mixtures with Klebsiella oxytoca, an NEC-positive-associated pathogen. Overall, the synbiotic mixtures of digested Tc-WS-NSP-ICE and high bacterial concentrations of L. acidophilus (5.57 × 109), B. breve (2.7 × 108 CFU/mL), and B. infantis (1.53 × 108) demonstrated higher (42.0%, 45.0%, 43.1%, respectively) ability to downregulate IL-8 compared to the sole use of Tc-WS-NSPs (24.5%), or the probiotics L. acidophilus (32.3%), B. breve (37.8%), or B. infantis (33.1%). The ability demonstrated by the Tc-WS-NSPs, the probiotics, and their synbiotics mixtures to downregulate IL-8 production in the presence of an NEC-positive-associated pathogen may be useful in the development of novel prophylactic agents against NEC.


Assuntos
Bifidobacterium breve , Colocasia , Enterocolite Necrosante , Probióticos , Simbióticos , Bifidobacterium , Bifidobacterium longum subspecies infantis , Citocinas , Enterocolite Necrosante/prevenção & controle , Humanos , Lactente , Recém-Nascido , Recém-Nascido Prematuro , Interleucina-8 , Lactobacillus acidophilus , Polissacarídeos/farmacologia , Probióticos/farmacologia , Probióticos/uso terapêutico , Água
16.
Microbiol Spectr ; 10(3): e0269621, 2022 06 29.
Artigo em Inglês | MEDLINE | ID: mdl-35499353

RESUMO

Vulvovaginal candidiasis (VVC) is a common clinical condition with symptoms and signs of vaginal inflammation in the presence of Candida species. At least one episode of VVC is experienced in up to 75% of women in the reproductive age group during their lifetime, and 5% to 8% of such women suffer from the chronic form. Most cases of VVC are still caused by C. albicans. However, the incidence of VVC cases by non-albicans Candida (NAC) species, such as C. parapsilosis, is continuously increasing. Despite the prevalence of VVC from NAC, little is known about these species and almost nothing about the mechanisms that trigger the VVC. Lactobacillus spp. are the most widely before represented microorganisms in the vaginal microbiota of healthy women. Here, cell-free supernatants (CFS) obtained from L. acidophilus, L. plantarum, L. rhamnosus, and L. reuteri were assessed for their effect on C. parapsilosis virulence traits. Moreover, we assessed if such an effect persisted even after the removal of the CFS (CFS preincubation effect). Moreover, a transwell coculture system was employed by which the relevant antifungal effect was shown to be attributable to the compounds released by lactobacilli. Our results suggest that lactobacilli can work (i) by reducing C. parapsilosis virulence traits, as indicated by the reduced fungal proliferation, viability, and metabolic activity, and (ii) by improving epithelial resistance to the fungus. Overall, these data suggest that, in the context of the vaginal microbiota, the lactobacilli may play a role in preventing the onset of mucosal C. parapsilosis infection. IMPORTANCE The incidence of VVC by non-albicans Candida (NAC) species, such as C. parapsilosis, is increasing. Treatment failure is common in NAC-VVC because some species are resistant or poorly susceptible to the antifungal agents normally employed. Research on C. parapsilosis's pathogenic mechanisms and alternative treatments are still lacking. C. albicans triggers the VVC by producing hyphae, which favor the loss of epithelial tolerance. Differently, C. parapsilosis only produces pseudohyphae. Hence, different virulence factors may trigger the VVC. Likewise, the therapeutic options could also involve different fungal targets. Substantial in vitro and in vivo studies on the pathogenicity mechanisms of C. parapsilosis are lacking. The data presented here ascribe a novel beneficial role to different Lactobacillus spp., whose CFS provides a postbiotic-like activity against C. parapsilosis. Further studies are needed to unravel the mechanisms involved in the bioactivities of such compounds, to better understand the role of single postbiotics in the CFS.


Assuntos
Candidíase Vulvovaginal , Síndrome de Fadiga Crônica , Antifúngicos/farmacologia , Antifúngicos/uso terapêutico , Candida , Candida albicans , Candida parapsilosis , Candidíase Vulvovaginal/tratamento farmacológico , Candidíase Vulvovaginal/microbiologia , Candidíase Vulvovaginal/patologia , Técnicas de Cocultura , Células Epiteliais , Síndrome de Fadiga Crônica/tratamento farmacológico , Feminino , Humanos , Lactobacillus , Lactobacillus acidophilus
17.
Molecules ; 27(9)2022 May 08.
Artigo em Inglês | MEDLINE | ID: mdl-35566377

RESUMO

Collagen has become popular in dietary supplements, beverages and sports nutrition products. Therefore, the aim of this study was to evaluate the possibility of using various doses of collagen and collagen hydrolysate to produce probiotic sheep's milk fermented with Lactobacillus acidophilus, Lacticaseibacillus casei, Lacticaseibacillus paracasei and Lacticaseibacillus rhamnosus. The effects of storage time, type and dose of collagen, and different probiotic bacteria on the physicochemical, organoleptic and microbiological properties of fermented sheep's milk at 1 and 21 days of refrigerated storage were investigated. The addition of collagen to sheep's milk increased the pH value after fermentation and reduced the lactic acid contents of fermented milk compared to control samples. After fermentation, the number of probiotic bacteria cells was higher than 8 log cfu g-1. In sheep's milk fermented by L. acidophilus and L. casei, good survival of bacteria during storage was observed, and there was no effect of collagen dose on the growth and survival of both strains. The addition of collagen, both in the form of hydrolysate and bovine collagen, resulted in darkening of the color of the milk and increased the sweet taste intensity of the fermented sheep's milk. However, the addition of hydrolysate was effective in reducing syneresis in each milk sample compared to its control counterpart.


Assuntos
Lactobacillus rhamnosus , Probióticos , Animais , Bovinos , Colágeno/análise , Fermentação , Lactobacillus acidophilus , Leite/química , Probióticos/farmacologia , Ovinos
18.
Sci Rep ; 12(1): 8491, 2022 05 19.
Artigo em Inglês | MEDLINE | ID: mdl-35589853

RESUMO

Nucleotide-binding oligomerization domain containing 2 (NOD2) is a critical regulator of immune responses within the gastrointestinal tract. This innate immune receptor is expressed by several cell types, including both hematopoietic and nonhematopoietic cells within the gastrointestinal tract. Vaccination targeting the gastrointestinal mucosal immune system is especially difficult due to both physical and mechanistic barriers to reaching inductive sites. The use of lactic acid bacteria is appealing due to their ability to persist within harsh conditions, expression of selected adjuvants, and manufacturing advantages. Recombinant Lactobacillus acidophilus (rLA) has shown great promise in activating the mucosal immune response with minimal impacts on the resident microbiome. To better classify the kinetics of mucosal vaccination with rLA, we utilized mice harboring knockouts of NOD2 expression specifically within CD11c + cells. The results presented here show that NOD2 signaling in CD11c + cells is necessary for mounting a humoral immune response against exogenous antigens expressed by rLA. Additionally, disruption of NOD2 signaling in these cells results in an altered bacterial microbiome profile in both control mice and mice receiving L. acidophilus strain NCK1895 and vaccine strain LaOVA.


Assuntos
Microbioma Gastrointestinal , Animais , Imunidade Humoral , Lactobacillus acidophilus/metabolismo , Camundongos , Proteína Adaptadora de Sinalização NOD2/metabolismo , Vacinação
19.
PLoS One ; 17(5): e0268953, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35613129

RESUMO

α-L-rhamnosidase catalyzes hydrolysis of the terminal α-L-rhamnose from various natural rhamnoglycosides, including naringin and hesperidin, and has various applications such as debittering of citrus juices in the food industry and flavonoid derhamnosylation in the pharmaceutical industry. However, its activity is lost at high temperatures, limiting its usage. To improve Lactobacillus acidophilus α-L-rhamnosidase stability, we employed molecular dynamics (MD) to identify a highly flexible region, as evaluated by its root mean square fluctuation (RMSF) value, and computational protein design (Rosetta) to increase rigidity and favorable interactions of residues in highly flexible regions. MD results show that five regions have the highest flexibilities and were selected for design by Rosetta. Twenty-one designed mutants with the best ΔΔG at each position and ΔΔG < 0 REU were simulated at high temperature. Eight designed mutants with ΔRMSF of highly flexible regions lower than -10.0% were further simulated at the optimum temperature of the wild type. N88Q, N202V, G207D, Q209M, N211T and Y213K mutants were predicted to be more stable and could maintain their native structures better than the wild type due to increased hydrogen bond interactions of designed residues and their neighboring residues. These designed mutants are promising enzymes with high potential for stability improvement.


Assuntos
Glicosídeo Hidrolases , Lactobacillus acidophilus , Sucos de Frutas e Vegetais , Glicosídeo Hidrolases/metabolismo , Hidrólise , Lactobacillus acidophilus/genética , Lactobacillus acidophilus/metabolismo , Temperatura
20.
Curr Microbiol ; 79(6): 165, 2022 Apr 21.
Artigo em Inglês | MEDLINE | ID: mdl-35445852

RESUMO

To demonstrate that the amount of extracellular polymeric substances (EPS) and the freeze-dried viability of probiotics are correlated. Three strains of probiotics including Lactiplantibacillus plantarum, Lactobacillus acidophilus, and Bifidobacterium bifidum were subjected to environmental challenges, such as temperature, pH, and carbon dioxide. The results indicated that the challenges could stimulate the EPS synthesis of the probiotics. The experimental correlation between the amount of synthesized EPS and the freeze-dried survival rate was also analyzed, and the viability of each of the three strains was represented by the following functions in which the equation of L. plantarum is y = - 0.0336x2 + 2.7059x - 14.849 with R2 = 0.9699, the B. bifidum's equation is y = - 0.0554x2 + 2.6243x - 13.654 with R2 = 0.9554, and the L. acidophilus's one was y = 0.0346x2 + 0.5862x - 9.1339 with R2 = 0.9733. This could be a new approach to determining the freeze-dried viability of probiotic strains based on the measured EPS content.


Assuntos
Matriz Extracelular de Substâncias Poliméricas , Probióticos , Liofilização , Lactobacillus acidophilus , Taxa de Sobrevida
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