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1.
J Food Sci ; 87(7): 3071-3083, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35669993

RESUMO

Lactic acid production from food waste via fermentation is environmentally sustainable. However, the characteristics of food waste fermentation to produce lactic acid are not well understood due to the complexity of food waste. This study aims to understand the effects of key variables on the characteristics of food waste fermentation to maximize lactic acid production. Food waste was enzymatically hydrolyzed and fermented by Lactobacillus pentosus. Key fermentation variables, including nitrogenous nutrient supplementation, initial sugar concentration, and pH, were investigated in batch fermentation to unveil their effects on fermentation titer, yield, and productivity. The results showed that supplementation of 0.25% (w/v%) yeast extract and peptone to the food waste fermentation media significantly improved fermentation titer and productivity, but further increase in the supplementation level did not improve fermentation. Increasing the initial sugar concentration from 40 g/L to 100 g/L increased the fermentation titer from 41.0 g/L to 93.0 g/L and productivity from 0.34 g/L/h to 0.76 g/L/h. pH 6.0 was the optimal pH for the fermentation. At the optimal conditions, food waste fermentation resulted in the highest fermentation titer, yield, and productivity of 106.7 g/L, 1.12 g/g, and 3.09 g/L/h, respectively. The high fermentation yield of 1.12 g/g might be explained by the extra lactic acid production from unidentified compounds in food waste hydrolysates. By applying fed-batch fermentation, the lactic acid concentration reached 157.0 g/L with a yield and overall productivity of 0.92 g/g and 2.0 g/L/h, respectively. Based on the mass balance, a total of 251 kg lactic acid was produced from 1000 kg food waste. PRACTICAL APPLICATION: Food waste is one of the largest municipal solid wastes in the US, and most food waste ends up in landfills, causing significant economic losses and environmental concerns. In this study, we developed a fermentation process to convert food waste into biorenewable lactic acid and demonstrated that food waste is a superior feedstock for fermentation due to its embedded nutrients. Moreover, due to the embedded nutrients in food waste, the supplementation of yeast extract and peptone to fermentation can be reduced by over 50%, which can reduce the operating cost of lactic acid fermentation on an industrial scale.


Assuntos
Lactobacillus pentosus , Eliminação de Resíduos , Suplementos Nutricionais , Fermentação , Concentração de Íons de Hidrogênio , Ácido Láctico , Nitrogênio , Peptonas , Açúcares
2.
Food Res Int ; 151: 110840, 2022 01.
Artigo em Inglês | MEDLINE | ID: mdl-34980379

RESUMO

The objective of this study was to investigate the antibacterial effect and mechanism of Lactobacillus pentosus pentocin against Bacillus cereus. The dynamic growth of B. cereus showed that the pentocin had strong antibacterial activity against the strain. The antibacterial mechanism focused on cytomembrane destruction, biofilms formation, DNA replication and protein synthesis of B. cereus. The scanning electron microscopy, transmission electron microscopy and flow cytometry analysis illustrated that the cytomembranes were destroyed, causing the leakage of internal cellular components. Transcriptome sequencing indicated that the genes (KinB, KinC and Spo0B) in two component systems signal pathway were down-regulated, which resulted in the inhibition of the spores and biofilms formation of B. cereus. The phosphorylation and autoinducer-2 import were inhibited by down-regulating the expression levels of LuxS and LsrB genes in quorum sensing signal pathway, which also suppressed biofilms formation of B. cereus. The K+ leakage activated the K+ transport channels by up-relating the genes (KdpA, KdpB and KdpC), promoting the entry of K+ from the extracellular. In addition, the pentocin interfered DNA replication and protein synthesis by regulating the genes associated with DNA replication (dnaX and holB), RNA degradation (cshA, rho, rnj, deaD, rny, dnaK, groEL and hfq) and ribosome function (rpsA, rpsO and rplS). In this article, we provide some novel insights into the molecular mechanism responsible for high antibacterial activity of the L. pentosus pentocin against B. cereus. And the pentocin might be a very promising natural preservative for controlling the B. cereus contaminations in foods.


Assuntos
Bacillus cereus , Lactobacillus pentosus , Antibacterianos/farmacologia , Bacillus cereus/genética , Perfilação da Expressão Gênica , Percepção de Quorum
3.
Fish Shellfish Immunol ; 120: 345-352, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34883257

RESUMO

Litopenaeus vannamei is one of the most productive shrimp species in the world. However, shrimp farming is suffering from adverse environmental conditions and disease outbreaks. Typically, Lactobacillus pentosus and Arthrospira platensis are used as substitutes for some antibiotics. In the present study, we assessed the effects of dietary supplements along with living bacteria or cell-free extracts of L. pentosus combined with A. platensis on the growth performance, immune response, intestinal microbiota, and disease resistance of L. vannamei against Vibrio alginolyticus. Shrimp fed L. pentosus live bacteria combined with A. platensis showed the best growth performance and lowest feed conversion rate. The supplementation diet with L. pentosus live bacteria and A. platensis could significantly enhance the trypsin activity in shrimp after the feeding trial. Given the lowest feed conversion rate in shrimp fed L. pentosus live bacteria combined with A. platensis, we reasonably speculated that the decrease in feed conversion rate may be related to the increase in trypsin activity. In addition, dietary cell-free extracts of L. pentosus combined with A. platensis enhanced the expression of immune-related genes after the feeding trial or challenge test. Moreover, results of the bacterial challenge test indicated that the shrimp fed cell-free extracts of L. pentosus combined with A. platensis diet resulted in the highest survival rate, which suggested that cell-free extracts of L. pentosus and A. platensis could improve the disease resistance against V. alginolyticus by up-regulating the expressions of immune-related genes. Dietary L.pentosus or A. platensis, or their combination, reduced the abundance of harmful bacteria, including Proteobacteria in shrimp intestine, which suggested that L. pentosus and A. platensis could improve the growth performance and health of shrimp by regulating the structure of the intestinal microbiota. The findings of this study demonstrated that L. pentosus live bacteria and A. platensis exerted synergistic effects on the growth performance and digestion in shrimp, while cell-free extracts of L. pentosus and A. platensis showed synergistic effects on the immune response and disease resistance of shrimp against V. alginolyticus.


Assuntos
Dieta/veterinária , Microbioma Gastrointestinal , Lactobacillus pentosus , Penaeidae , Probióticos , Spirulina/química , Ração Animal/análise , Animais , Resistência à Doença , Imunidade Inata , Penaeidae/crescimento & desenvolvimento , Penaeidae/imunologia , Tripsina
4.
Braz J Microbiol ; 53(1): 327-340, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-34816387

RESUMO

Noteworthy properties of biosurfactant (BS) are fascinating scientific fraternity to explore them for food, medicinal, cosmetic, or pharmaceutical etc. applications. Newer products intended for pharmaceutical purposes are mandatory to go through pragmatic evaluation protocols. BS, being less cytotoxic, offers an ideal candidature for widespread applications in the healthcare sector. The goal of the current study was the isolation, physico-chemical characterization, and safety assessment of cell-associated biosurfactant (CABS) from Lactobacillus pentosus NCIM 2912. The culture was grown in a 3-L fermentor to produce CABS from the cell pellets through procedures like centrifugation, filtration, dialysis, column chromatography, and freeze-drying. Further, physical properties like surface tension (ST), critical micelle concentration (CMC), contact angle (CA), emulsification activity, stability of emulsion (height of emulsion, the extent of coalescence, and appearance), and ionic character of CABS were evaluated. Analytical characterization through TLC, FTIR, NMR, and GC-MS was carried out. The physico-chemical characterization revealed CABS as an anionic, multicomponent glycolipopeptide having a hydrophobic chain comprising butanoic acid (C4), decanoic acid (C10), undecanoic acid (C11), tridecanoic acid (C13), hexadecenoic acid (C16), and octadecanoic acid (C18). The oil-in-water (O/W) emulsions formed by CABS with various oils (olive, sesame, soybean, coconut) were stabilized up to the 7th day of storage and were analogous with polysorbate 80 (emulsifier/defoamer used in food industries). The O/W emulsions are quite stable at room temperature with no evidence of coalescence of droplets around 1 week. The cytotoxicity of CABS was evaluated through MTT (3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide) assay. Cytotoxicity study performed on the human embryonic kidney (HEK 293), mouse fibroblast ATCC L929 and human epithelial type (HEP-2) cell lines recorded viability of 90.3 ± 0.1%, 99.2 ± 0.43, and 94.3 ± 0.2% respectively. The toxicity of the BS was comparable to that of the commercially used rhamnolipid sample. Thus, CABS derived from L. pentosus NCIM 2912 pose promising applications in the pharmaceutical, food industries acquiescently. The multifunctional potential of the incredibly versatile microbial product like BS from lactic acid bacteria (LAB) certainly contributes to wider avenues for varied industries.


Assuntos
Lactobacillus pentosus , Animais , Emulsificantes , Emulsões/química , Células HEK293 , Humanos , Lactobacillus pentosus/metabolismo , Camundongos , Tensoativos/química , Tensoativos/farmacologia
5.
J Hazard Mater ; 423(Pt A): 127101, 2022 02 05.
Artigo em Inglês | MEDLINE | ID: mdl-34488094

RESUMO

In this study, a novel beta-cypermethrin (beta-cyp)-degrading strain Lactobacillus pentosus 3-27 (LP3-27) was screened from beta-cyp-contaminated silage. The strain could degrade 96% of beta-cyp (50 mg/L) in MSM medium after 4 d of culture, while the strain lost its degradation ability when the beta-cyp concentration reached 250 mg/L. The effects of LP 3-27 on fermentation, bacterial community, and bioremediation of contaminated alfalfa silage at two dry matter (DM) contents were studied. The results showed that inoculation with LP3-27 not only degraded beta-cyp, but also improved the fermentation quality of alfalfa silage after 60 d of ensiling. Meanwhile, L. pentosus dominated the bacterial community during ensiling in LP3-27 inoculated silages, whereas Pediococcus acidilactici was the dominant species in the control silage. LP3-27 inoculation also simplified the bacterial interaction networks of ensiled alfalfa. Beta-cyp degradation was positively correlated with L. pentosus in LP- inoculated silages, which confirmed the function of beta-cyp degradation by L. pentosus. In addition, higher beta-cyp degradation was observed in silage with 35% versus 43% DM. In summary, strain LP3-27 could be used as a candidate inoculum for bioremediation of beta-cyp-contaminated silage and to produce safe silage for animal production.


Assuntos
Lactobacillus pentosus , Silagem , Animais , Biodegradação Ambiental , Fermentação , Medicago sativa , Piretrinas , Silagem/análise
6.
Food Funct ; 12(19): 9456-9465, 2021 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-34606528

RESUMO

Exopolysaccharides (EPSs) derived from Lactobacilli have important physiological effects and are commonly used as new prebiotics. We identified and studied a new Lactobacillus strain, YY-112, isolated from waxberry (Myrica rubra). This strain, identified as Lactobacillus pentosus, tolerates acids, bile salts, and artificial digestive fluids. The EPS derived from this strain weighed 5.9 × 104 Da and contained glucose, mannose, glucosamine, galactose, and rhamnose at 62.69 : 85.85 : 2.46 : 2.92 : 1.00 molar ratios. We found that the EPS from this strain increased the ratio of Bacteroidetes to Firmicutes and decreased the relative abundance of Proteobacteria, especially Escherichia-Shigella, when added to a simulated gastrointestinal system in vitro. After analysing the short-chain fatty acids, we found that this EPS promoted the production of acetic acid, propionic acid, and butyric acid, and reduced the ratio of acetic acid to propionic acid. We conclude that Lactobacillus pentosus YY-112 is a potential probiotic strain with EPS that is beneficial for the intestinal microbiota and short-chain fatty acid production.


Assuntos
Microbioma Gastrointestinal , Trato Gastrointestinal/microbiologia , Lactobacillus pentosus/metabolismo , Polissacarídeos Bacterianos , Prebióticos , Bacteroidetes/crescimento & desenvolvimento , Bochecha , Digestão , Ácidos Graxos Voláteis/metabolismo , Fermentação , Firmicutes/crescimento & desenvolvimento , Trato Gastrointestinal/metabolismo , Humanos , Técnicas In Vitro , Lactobacillus pentosus/classificação , Filogenia , Polissacarídeos Bacterianos/biossíntese , Polissacarídeos Bacterianos/química , Polissacarídeos Bacterianos/isolamento & purificação , Polissacarídeos Bacterianos/metabolismo , Proteobactérias/crescimento & desenvolvimento
7.
Fish Shellfish Immunol ; 117: 124-135, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34343542

RESUMO

This study aims to assess and determine the oral-administration of probiotic, Lactobacillus pentosus BD6 on growth performance, immunity and disease resistance of white shrimp, Litopenaeus vannamei. Lac. pentosus BD6 effectively inhibited the growth of aquatic pathogens, which was used in the test. Shrimp were fed with the control diet (without probiotic supplement) for 60 days and the probiotic-containing diets at 107, 108, 109, and 1010 cfu kg-1, respectively. Shrimp fed with the diet containing probiotic at the doses of 109-10 cfu kg-1 showed significant increase in growth performance as well as feed efficiency than that of the control. After a challenge test with Vibrio alginolyticus, shrimp fed with a probiotic diet at a dose of 1010 cfu kg-1 showed a significantly lower mortality as compared to the control and that of shrimp fed the diet containing probiotic at the levels up to 107-8 cfu kg-1. In addition, a therapeutic potential of Lac. pentosus BD6 was discovered because the cumulative mortalities of shrimp fed with probiotic and pathogen V. parahaemolyticus simultaneously were significantly lower when compared to control shrimp. Probiotic in diet at a dose of 109-10 cfu kg-1 significantly increased PO activity of shrimp, while shrimp receiving probiotic at the doses of 108-10 cfu kg-1 showed significant increase in lysozyme activity and phagocytic activity. Shrimp fed with the diet containing probiotic at the level of 1010 cfu kg-1 also indicated higher gene expression of prophenoloxidase (proPO) I, but not proPO II, lipopolysaccharide and ß-1,3-glucan-binding protein and penaeidin 4. Analysis of the bacterial microbiota of the shrimp intestine revealed that oral administration of probiotic increased the relative abundance of beneficial bacteria and reduced the abundance of harmful pathogenic bacteria in the gut flora of shrimp. Despite no statistically significant difference, an analysis of microbial diversity recorded higher species richness, Shannon-Weaver diversity index and evenness in the probiotic group, compared to the control group. It was concluded that Lac. pentosus BD6 has great antibacterial ability against a wide range of pathogens and has therapeutic potential to reduce the mortality of shrimp infected with V. parahaemolyticus. Additionally, dietary Lac. pentosus BD6 at the level of 1010 cfu kg-1 was recommended to improve growth performance, immunity and disease resistance of shrimp against V. alginolyticus.


Assuntos
Lactobacillus pentosus , Penaeidae , Probióticos/administração & dosagem , Vibrioses/prevenção & controle , Vibrio alginolyticus , Administração Oral , Animais , Catecol Oxidase/imunologia , Resistência à Doença , Precursores Enzimáticos/imunologia , Microbioma Gastrointestinal , Expressão Gênica , Hemócitos/imunologia , Hemolinfa/imunologia , Muramidase/imunologia , Penaeidae/genética , Penaeidae/crescimento & desenvolvimento , Penaeidae/imunologia , Penaeidae/microbiologia , Fagocitose , Vibrioses/mortalidade , Vibrioses/veterinária
8.
Int J Mol Sci ; 22(7)2021 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-33915904

RESUMO

Particulate matter (PM) is a significant environmental pollutant that promotes respiratory diseases, including lung injury and inflammation, by inducing oxidative stress. Rhynchosia nulubilis (black soybean) is traditionally used to prevent chronic respiratory disease via inducing antioxidant and anti-inflammatory effects. To investigate the effects of Lactobacillus pentosus SC65 fermented GR (GR-SC65) and Pediococcus pentosaceus ON81A (GR-ON81A) against PM-induced oxidative stress and cell death in A549 cells, we performed the 2-7-dichlorodihydrofluorescein diacetate and cell counting kit-8 assays, as well as Hoechst 33342 and propidium iodide staining and western blotting. GR-SC65 showed the highest total polyphenolic contents and 1,1-diphenyl-2-picrylidrazil radical scavenging activity among lactic acid bacteria-fermented GRs (p < 0.001 vs. GR). Four soy peptides, ß-conglycinin breakdowns (INAENNQRNF, ISSEDKPFN, LAFPGSAQAVEK, and LAFPGSAKDIEN), were detected in GR-SC65, but not in GR. In GR-SC65, PM-induced A549 cell death was less than that observed in GR-ON81A and GR (p < 0.001 vs. PM-treated group). GR-SC65 significantly decreased intracellular reactive oxidative species (ROS) when compared with PM (*** p < 0.001 vs. PM). GR-SC65 decreased the levels of BAX, active caspase-9, -3, and poly ADP-ribose polymerase (PARP) proteins (#p < 0.01, ###p < 0.001 vs. PM), while increasing the level of BCL-2 protein, a mitochondrial anti-apoptotic protein (###p < 0.001 vs. PM). Our findings indicate that GR-SC65 inhibited PM-induced cell death by suppressing the levels of ROS, active caspase-9 and -3, and PARP proteins, while enhancing the level of BCL-2 protein in type II alveolar epithelial A549 cells. Therefore, GR-SC65 might be a potential therapeutic and preventive agent against PM-induced lung injury.


Assuntos
Células Epiteliais Alveolares/efeitos dos fármacos , Lactobacillus pentosus/metabolismo , Pneumopatias/prevenção & controle , Extratos Vegetais/uso terapêutico , Soja/metabolismo , Células A549 , Apoptose/efeitos dos fármacos , Avaliação Pré-Clínica de Medicamentos , Fermentação , Humanos , Pneumopatias/etiologia , Estresse Oxidativo/efeitos dos fármacos , Material Particulado/efeitos adversos , Fitoterapia , Extratos Vegetais/farmacologia
9.
Appl Biochem Biotechnol ; 193(5): 1496-1512, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33484444

RESUMO

In this study, Box-Behnken design was applied to optimize the initial concentrations of 4 cations for L-lactic acid production from fructose by homologous batch fermentation of Lactobacillus pentosus cells. The optimum initial cation concentrations were obtained as 6.542 mM Mg2+, 3.765 mM Mn2+, 2.397 mM Cu2+, and 3.912 mM Fe2+, respectively. The highest L-lactic acid yield and productivity were obtained as 0.935 ± 0.005 g/g fructose and 1.363 ± 0.021 g/(L × h), respectively, with a maximum biomass concentration of 7.97 ± 0.17 g/L. The effectiveness of the optimization by Box-Behnken design was confirmed based on the small errors between predicted results and experimental results shown as 0.3%, - 0.2%, and - 1.2%, respectively. The quadratic models with high accuracy and reliability can be applied to mathematically forecasted the fermentation performance. After the optimization, the lactic acid yield and productivity were significantly improved by 3.7% and 21.0%, respectively.


Assuntos
Frutose/metabolismo , Ácido Láctico/metabolismo , Lactobacillus pentosus/metabolismo , Biomassa , Cátions , Modelos Teóricos
10.
Biotechnol Appl Biochem ; 68(4): 809-822, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32738151

RESUMO

Parametric optimization always plays important roles in bioengineering systems to obtain a high product yield under the proper conditions. The parametric conditions of lactic acid production by homologous batch fermentation of Lactobacillus pentosus cells was optimized by the Box-Behnken design. The highest l-lactic acid yield was obtained as 0.836 ± 0.003 g/g glucose with the productivity of 0.906 ± 0.003 g/(L × H) under the optimum conditions of 34.7 °C, pH 6.2, 148 rpm agitation speed, and 9.3 g/L nitrogen source concentration determined by quadratic response surface with high accuracy. The adequate kinetic models of cell growth rate, lactic production rate, and glucose consumption rate were also established to describe the fermentation behavior of L. pentosus cells with the correlation coefficients of 09985, 0.9990, and 0.9989, respectively.


Assuntos
Técnicas de Cultura Celular por Lotes , Ácido Láctico/biossíntese , Lactobacillus pentosus/crescimento & desenvolvimento , Modelos Biológicos , Cinética
11.
Food Microbiol ; 94: 103652, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33279077

RESUMO

The Lactobacillus plantarum and Lactobacillus pentosus genotypes existing in industrial-scale cucumber fermentations were defined using rep-PCR-(GTG)5. The ability of each genotype to ferment cucumbers under various conditions was evaluated. Rep-PCR-(GTG)5 was the technique capable of illustrating the most intraspecies discrimination compared to the sequencing of housekeeping genes (recA, dnaK, pheS and rpoA), MLST and RAPD with primers LP1, OPL5, M14 and COC. Ten genotypic clusters were defined for the 199 L. pentosus tested and three for the 17 L. plantarum clones. The ability of the 216 clones genotyped and 37 additional cucumber fermentation isolates, of the same species, to rapidly decrease the pH of cucumber juice medium under various combinations of sodium chloride (0 or 6%), initial pH (4.0 or 5.2) and temperatures (15 or 30 °C) was determined using a fractional factorial screening design. A reduced fermentation ability was observed for the L. plantarum strains as compared to L. pentosus, except for clone 3.2.8, which had a ropy phenotype and aligned to genotypic cluster A. L. pentosus strains belonging to three genotypic clusters (B, D and J) were more efficient in cucumber juice fermentation as compared to most L. plantarum strains. This research identified three genetically diverse L. pentosus strains and one L. plantarum as candidates for starter cultures for commercial cucumber fermentations.


Assuntos
Cucumis sativus/microbiologia , Lactobacillus pentosus/genética , Lactobacillus plantarum/genética , Fermentação , Microbiologia de Alimentos , Genótipo , Lactobacillus pentosus/classificação , Lactobacillus pentosus/isolamento & purificação , Lactobacillus pentosus/metabolismo , Lactobacillus plantarum/classificação , Lactobacillus plantarum/isolamento & purificação , Lactobacillus plantarum/metabolismo , Fenótipo , Técnica de Amplificação ao Acaso de DNA Polimórfico , Cloreto de Sódio/metabolismo
12.
J Ethnopharmacol ; 265: 113236, 2021 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-32750462

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Rhynchosia nulubilis (black soybean) has many applications in oriental medicine. It is traditionally used to treat disease related with high blood pressure, diabetes, inflammation, and osteoporosis. Furthermore, fermented soybean foods have traditionally been used for immunity enhancement in East Asia. However, the anti-inflammatory effects of germinated R. nulubilis (GR) against delayed-type hypersensitivity (DTH) are not fully understood. AIM OF STUDY: This study aimed to investigate the anti-inflammatory effects of germinated Rhynchosia nulubilis (GR) fermented with the lactic acid bacterium Lactobacillus pentosus SC65 (GR-SC65) isolated from pickled burdock. MATERIALS AND METHODS: We investigated the effects of GR-SC65 (300 mg/kg/day) on ear thickness and immune cell infiltration in DNFB-induced DTH in mice. We used dexamethasone (3 mg/kg) as a reference drug. Changes in infiltration of CD4+ and CD8+ T cells and NK cells were examined by immunohistochemistry. In addition, we investigated cytokine and chemokine production related to DTH using reverse transcription-polymerase chain reaction. We also investigated DTH-related cytokine production using lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells. RESULTS: Two lactic acid bacterial strains (Lactobacillus pentosus SC65 and Pediococcus pentosaceus ON81A) were selected for fermenting GR due to their high 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity. The total polyphenol contents (TPCs) in GR-SC65 and GR-ON81A were higher than that in unfermented GR (∗∗∗P < 0.001 vs. GR). Content of daidzein, glycitein, and genistein, the deglycosylated form of isoflavonoids, was higher in GR-SC65 than in unfermented GR. The ethanol extracts of GR-SC65 exerted a stronger anti-inflammatory activity than GR by inhibiting pro-inflammatory cytokines, such as tumor necrosis factor (TNF), interleukin-6 (IL-6), and interleukin-1ß (IL-1ß) in LPS-induced RAW264.7 macrophages. GR-SC65 reduced 1-fluoro-2,4-dinitrofluorobenzene (DNFB)-induced ear swelling and hyperplasia as well as vascular permeability. Fewer infiltrated CD4+ and CD8+ T cells were observed in the ear tissue of the GR-SC65-treated mice than those of the unfermented GR-treated mice. Furthermore, fewer infiltrated NK cells were observed in the GR-SC65 treated mice, than in the GR-treated mice. GR-SC65 significantly diminished the levels of CCL5 and COX-2 mRNAs and increased the level of IL-10 mRNA. CONCLUSIONS: These data suggest that GR-SC65 can be used as a health supplement or a prophylactic against delayed-type hypersensitive inflammatory disease.


Assuntos
Anti-Inflamatórios/farmacologia , Hipersensibilidade Tardia/prevenção & controle , Lactobacillus pentosus , Soja/química , Animais , Linfócitos T CD4-Positivos/imunologia , Linfócitos T CD8-Positivos/imunologia , Citocinas/metabolismo , Dexametasona/farmacologia , Dinitrofluorbenzeno , Feminino , Fermentação , Inflamação/tratamento farmacológico , Macrófagos/efeitos dos fármacos , Macrófagos/patologia , Camundongos , Camundongos Endogâmicos C57BL , Células RAW 264.7
13.
Food Funct ; 11(12): 10913-10924, 2020 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-33242049

RESUMO

Fermenting garlic and onion provides the advantages of storage life extension, anti-oxidative and anti-diabetic activities, and their metabolite, allyl mercaptan, offers a strong aroma and various health benefits. Here, we report the probiotic properties of Lactobacillus pentosus SMB718 isolated from Korean traditional paste and its high allyl mercaptan productivity in garlic and onion fermentation. This strain was safe for use in food fermentation, as it was a non-biogenic amine producer and non-hemolytic. It showed high stability under simulated human gastrointestinal conditions and good adhesion ability to intestinal epithelial cells, including both Caco-2 and HT-29. This strain had antioxidant and anti-microbial activities. In addition, the heat-killed cells and lysate exerted anti-inflammatory effects on both LPS-stimulated RAW 264.7 cells and mouse macrophages by inhibition of pro-inflammatory cytokines and induction of anti-inflammatory cytokines. Furthermore, this strain possessed good fermentation properties in garlic and onion-enriched radish juice (GORJ); it grew well decreasing the pH and provided a rich aroma compound during fermentation. When L. pentosus SMB718 was used as a starter in garlic and onion fermentation, a significantly higher amount of allyl mercaptan (344 ppb) was produced compared with that produced by the type strain (82 ppb). In conclusion, L. pentosus SMB718 can be used as a beneficial probiotic starter for better flavor production in the fermentation of allium species plants.


Assuntos
Fermentação , Alho/química , Lactobacillus pentosus/fisiologia , Cebolas/química , Probióticos , Compostos de Sulfidrila/metabolismo , Animais , Anti-Infecciosos/farmacologia , Anti-Inflamatórios , Antioxidantes/farmacologia , Células CACO-2 , Citocinas , Células Epiteliais , Alho/microbiologia , Células HT29 , Humanos , Camundongos , Cebolas/microbiologia , Células RAW 264.7
14.
Bioresour Technol ; 318: 124260, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33091689

RESUMO

Lactic acid is one of the essential platform chemicals, and despite the availability of a range of downstream processes, its effective recovery is still elusive. A phase partitioning process using n-butanol and a chaotropic salt ammonium sulphate was developed to recover lactic acid from the fermentation broth of Lactobacillus pentosus SKL-18. During the optimization of various process parameters, the extraction medium's pH was found to be critical, with 2.5 being the best. The optimized process resulted in a lactic acid yield of 86% and found it to be 93% pure. The purity and characteristics of lactic acid were confirmed by FTIR and NMR spectra. This solvent-based extraction procedure is an economical and straightforward downstream process for purifying lactic acid produced from agro- and bakery-residues. The pure lactic acid can further be used for enzymatic synthesis of high value-added product PLA, a biodegradable and biocompatible plastics.


Assuntos
Ácido Láctico , Lactobacillus pentosus , 1-Butanol , Fermentação
15.
J Endocrinol ; 247(2): 169-181, 2020 11.
Artigo em Inglês | MEDLINE | ID: mdl-33032263

RESUMO

Intestinal mucosal barrier dysfunction is closely related to the pathogenesis of nonalcoholic steatohepatitis (NASH). Gut immunity has been recently demonstrated to regulate gut barrier function. The Lactobacillus pentosus strain S-PT84 activates helper T cells and natural killer/natural killer T cells. In this study, we examined the effect of S-PT84 on NASH progression induced by high-cholesterol/high-fat diet (CL), focusing on the immune responses involved in gut barrier function. C57BL/6 mice were fed a normal chow or CL diet with or without 1 × 1010 S-PT84 for 22 weeks. S-PT84 administration improved hepatic steatosis by decreasing triglyceride and free fatty acid levels by 34% and 37%, respectively. Furthermore, S-PT84 inhibited the development of hepatic inflammation and fibrosis, suppressed F4/80+ macrophage/Kupffer cell infiltration, and reduced liver hydroxyproline content. Administration of S-PT84 alleviated hyperinsulinemia and enhanced hepatic insulin signalling. Compared with mice fed CL diet, mice fed CL+S-PT84 had 71% more CD11c-CD206+ M2 macrophages, resulting in a significantly decreased M1/M2 macrophage ratio in the liver. Moreover, S-PT84 inhibited the CL diet-mediated increase in intestinal permeability. Additionally, S-PT84 reduced the recruitment of interleukin-17-producing T cells and increased the levels of intestinal tight junction proteins, including zonula occludens-1, occludin, claudin-3, and claudin-7. In conclusion, our findings suggest that S-PT84 attenuates diet-induced insulin resistance and subsequent NASH development by maintaining gut permeability. Thus, S-PT84 represents a feasible approach to prevent the development of NASH.


Assuntos
Lactobacillus pentosus/fisiologia , Hepatopatia Gordurosa não Alcoólica/microbiologia , Animais , Microbioma Gastrointestinal/fisiologia , Inflamação/microbiologia , Inflamação/terapia , Interleucina-17/metabolismo , Mucosa Intestinal/metabolismo , Mucosa Intestinal/microbiologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Hepatopatia Gordurosa não Alcoólica/terapia
16.
J Food Sci ; 85(10): 3487-3497, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32893884

RESUMO

Seven candidates for starter cultures for cucumber fermentations belonging to the Lactobacillus pentosus and Lactobacillus plantarum species were characterized based on physiological features desired for pickling. The isolates presented variable carbohydrate utilization profile on API® 50CHL test strips. The L. pentosus strains were unable to utilize d-xylose in MRS broth or the M medium. The lactobacilli were unable to produce histamine, tyramine, putrescine, and cadaverine in biogenic amine broth containing the necessary precursors. Production of d-lactic acid by the lactobacilli, detected enzymatically, was stimulated by growth in MRS broth as compared to cucumber juice medium (CJM). The lactobacilli utilized malic acid in the malate decarboxylase medium. Exopolyssacharide biosynthesis related genes were amplified from the lactobacilli. A sugar type-dependent-ropy phenotype was apparent for all the cultures tested in MRS and CJM. The genes associated with bacteriocin production were detected in the lactobacilli, but not the respective phenotypes. The antibiotic susceptibility profile of the lactobacilli mimics that of other L. plantarum starter cultures. It is concluded that the lactobacilli strains studied here are suitable starter cultures for cucumber fermentation. PRACTICAL APPLICATION: The availability of such starter cultures enables the implementation of low salt cucumber fermentations that can generate products with consistent biochemistry and microbiological profile.


Assuntos
Cucumis sativus/microbiologia , Microbiologia de Alimentos/métodos , Lactobacillus pentosus/metabolismo , Lactobacillus plantarum/metabolismo , Cloreto de Sódio/análise , Bacteriocinas/análise , Bacteriocinas/metabolismo , Aminas Biogênicas/metabolismo , Cucumis sativus/química , Meios de Cultura/química , Meios de Cultura/metabolismo , Fermentação , Lactobacillus pentosus/crescimento & desenvolvimento , Lactobacillus plantarum/crescimento & desenvolvimento , Malatos/análise , Malatos/metabolismo , Putrescina/metabolismo , Cloreto de Sódio/metabolismo , Tiramina/análise , Tiramina/metabolismo
17.
Biosci Biotechnol Biochem ; 84(9): 1921-1935, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32463340

RESUMO

Awa-bancha is a post-fermented tea produced in Naka and Kamikatsu, Tokushima, Japan. We investigated the lactic acid bacteria in each stage of production of Awa-bancha and evaluated the relationships with the components. Lactic acid bacteria were isolated from tea leaves cultured with de Man, Rogosa, and Sharpe (MRS) agar plates, and the species were identified by homology of the 16 S rRNA gene and multiplex polymerase chain reaction (PCR) of the recA gene to distinguish the Lactobacillus plantarum group. As a result, a variety of species were isolated from the raw tea leaves, and Lactobacillus pentosus was isolated most frequently after anaerobic fermentation. Regarding the tea leaf components, organic acids, such as lactic acid, increased, free amino acids decreased, and catechins changed owing to anaerobic fermentation. Our results suggest that the microbial flora mainly composed of L. pentosus is important in the anaerobic fermentation process for flavor formation of Awa-bancha.


Assuntos
Fermentação , Lactobacillus pentosus/metabolismo , Chá/microbiologia , Anaerobiose , Lactobacillus pentosus/genética , RNA Ribossômico 16S/genética , Recombinases Rec A/genética , Paladar
18.
J Korean Med Sci ; 35(18): e128, 2020 May 11.
Artigo em Inglês | MEDLINE | ID: mdl-32383366

RESUMO

BACKGROUND: Recent studies have shown that oral administration of probiotics may improve the immune imbalance caused by dysbiosis of the gut microbiome in atopic dermatitis (AD). This study aimed to investigate the clinical and immunological effects of Lactobacillus pentosus in children with mild to moderate AD. METHODS: Children aged 2-13 years with AD were randomized to receive either 1.0 × 1010 colony-forming units of L. pentosus or placebo, daily, for 12 weeks. The clinical severity of AD and transepidermal water loss were evaluated. Blood eosinophil counts, serum total immunoglobulin E (IgE), and cytokine levels were measured. The diversity and composition of the gut microbiota were also analyzed. RESULTS: Eighty-two children were recruited, and 41 were assigned to the probiotics intervention group. The mean scoring of atopic dermatitis (SCORAD) indices at baseline were 30.4 and 34.3 for the probiotics and placebo groups, respectively. At week 12, the mean indices were 23.6 and 23.1 for the probiotics and placebo groups, respectively. Clinical severity decreased significantly over time in both groups, with no significant difference between the two groups. In both groups, there were no significant differences in cytokine levels, microbial diversity, or the relative abundance of the gut microbiota at week 12 compared with the corresponding baseline values. The mean subjective scores of SCORAD indices after intervention for the probiotics group were significantly lower than those for the placebo group in IgE sensitized AD (P = 0.019). CONCLUSION: Our results show improved symptoms in the probiotics and placebo groups, and we could not find additional effects of L. pentosus in AD. However, the mean subjective scores of SCORAD indices for the probiotics group are significantly improved compared with those for the placebo group in allergen-sensitized AD.


Assuntos
Alérgenos/imunologia , Dermatite Atópica/terapia , Lactobacillus pentosus/imunologia , Adolescente , Criança , Pré-Escolar , Citocinas/sangue , Dermatite Atópica/imunologia , Dermatite Atópica/patologia , Método Duplo-Cego , Eosinófilos/citologia , Eosinófilos/metabolismo , Fezes/microbiologia , Feminino , Microbioma Gastrointestinal , Humanos , Imunoglobulina E/sangue , Contagem de Leucócitos , Masculino , Efeito Placebo , Probióticos/administração & dosagem , Índice de Gravidade de Doença
19.
Genomics ; 112(5): 3142-3149, 2020 09.
Artigo em Inglês | MEDLINE | ID: mdl-32450257

RESUMO

Lactic acid bacteria have been attracting increased attentions recent years because of harboring probiotic properties. In present study, a Lactobacillus pentosus strain ZFM94 was screened from healthy infant feces and its probiotic characteristics were investigated. We found that ZFM94 was resistant to environmental stresses (temperature, pH and NaCl), tolerant to gastrointestinal juice and bile salts, with inhibitory action against pathogens and capacity of folate production etc. Additionally, complete genome sequence of the strain was analyzed to highlight the probiotic features at genetic level. Genomic characteristics along with the experimental studies is critically important for building an appropriate probiotic profile of novel strains. Genes that correspond to phenotypes mentioned above were identified. Moreover, genes potentially related to its adaptation, such as carbon metabolism and carbohydrate transporter, carbohydrate-active enzymes, and a novel gene cluster RaS-RiPPs, were also revealed. Together, ZFM94 could be considered as a potential probiotic candidate.


Assuntos
Genoma Bacteriano , Lactobacillus pentosus/genética , Probióticos , Antibacterianos/metabolismo , Aderência Bacteriana , Metabolismo dos Carboidratos/genética , Carbono/metabolismo , Ácido Fólico/biossíntese , Suco Gástrico , Genômica , Humanos , Lactente , Lactobacillus pentosus/enzimologia , Lactobacillus pentosus/isolamento & purificação , Lactobacillus pentosus/metabolismo , Proteínas de Membrana Transportadoras/genética , Peptídeos/genética , Filogenia , Estresse Fisiológico
20.
Biosci Biotechnol Biochem ; 84(8): 1706-1713, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32316868

RESUMO

The enhancing effects of yeasts on the viability of lactic acid bacteria (LAB) under acidic conditions were investigated. Meyerozyma guilliermondii, coaggregative with both LAB strains under acidic conditions, significantly enhanced the viability of Lactobacillus pentosus and L. paracasei in pH 3.0 lactic acid (LA) buffer at 10°C (p < 0.05). Non-coaggregative yeasts (Saccharomyces cerevisiae, Schizosaccharomyces pombe, and Cyberlindnera saturnus) also significantly enhanced the LAB viability (p < 0.05), and physical contact between LAB and yeasts was not essential for the viability-enhancing effect, indicating that the coaggregation had no relation to the enhancing mechanism. Although yeast metabolites and LA assimilation had no enhancing effect, hydrogen peroxide (H2O2) decreased after yeast coincubation, and H2O2 elimination improved L. pentosus viability. H2O2 elimination alone did not sufficiently improve L. paracasei viability, but the addition of antioxidants was effective. These results suggest that the antioxidant activity of yeast increased the LAB viability under acidic conditions.


Assuntos
Lactobacillus paracasei/fisiologia , Lactobacillus pentosus/fisiologia , Saccharomyces cerevisiae/fisiologia , Saccharomycetales/fisiologia , Schizosaccharomyces/fisiologia , Meios de Cultura/química , Fermentação , Microbiologia de Alimentos , Humanos , Peróxido de Hidrogênio/análise , Peróxido de Hidrogênio/metabolismo , Concentração de Íons de Hidrogênio , Viabilidade Microbiana , Simbiose/fisiologia
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