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1.
J Mass Spectrom ; 57(11): e4894, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36369744

RESUMO

Lanolin, also known as wool wax, is derived from sheep and has diverse applications in food, cosmetic, textile and lubricant industries. Owing to its direct contact with human, there is a need of studying pesticide residues as contaminants in lanolin. The present study describes a single novel hyphenated gas chromatography-tandem mass spectrometry (GC-MS/MS) technique for the quantification of 14 organophosphorus (OP) pesticides (tecnazene, propetamphos, diazinon, dichlofenthion, chlorpyrifos methyl, fenchlorphos, malathion, chlorpyrifos, pirimiphos ethyl, bromophos ethyl, tetrachlorvinphos, ethion, phosalone, and coumaphos) in lanolin using electrospray ionization (EI) with multiple reaction monitoring (MRM) acquisition mode. The method is simple in terms of sample preparation steps based on matrix solid-phase dispersion (MSPD). The method was found to be linear over the analytical range of 0.05-2.0 µg/g, with acceptable coefficient of determination (r2  ≥ 0.99) for all the 14 pesticides. The limit of detection (LOD) and limit of quantification (LOQ) of the method were found to be less than 0.05 and 0.1 µg/g, respectively, for all the 14 OP pesticide residues. The precision and accuracy of the method were found to be within the acceptable limits, that is, recoveries in the range of 83.5%-104.1% with less than 12.5% of relative standard deviation for all the pesticides. The multiresidue method for estimating pesticide residues employing GC-MS/MS technique will be useful for OP pesticide levels in large number of lanolin samples.


Assuntos
Clorpirifos , Resíduos de Praguicidas , Praguicidas , Ovinos , Animais , Humanos , Praguicidas/análise , Resíduos de Praguicidas/análise , Espectrometria de Massas em Tandem/métodos , Lanolina/análise , Lanolina/química , Compostos Organofosforados/análise , Cromatografia Gasosa-Espectrometria de Massas/métodos , Clorpirifos/análise
2.
Artigo em Inglês | MEDLINE | ID: mdl-36191441

RESUMO

4,4-Dimethyl-substituted sterols are bioactive minor sterols of most animal fats and plant oils, but higher shares are present in lanolin (wool grease). Here, the isolation of the 4,4-dimethyl-substituted sterols dihydrolanosterol and lanosterol from lanolin by countercurrent chromatography (CCC) is described. An initial examination of the hexane extract of saponified lanolin showed the presence of relatively high portions of fatty alcohols which were known to co-elute with the target analytes in CCC. Hence, fatty alcohols were precipitated by urea complexation. Unexpectedly, 4,4-dimethyl-substituted sterols were also found in the crystalline fraction, while cholesterol and other desmethylsterols were detected in the liquid phase. Urea complexation represented a useful preparative method for the separation of desmethylsterols and 4,4-dimethyl-substituted sterols from lanolin. Shake flask experiments of 4,4-dimethyl-substituted sterols and fatty alcohols with 14 biphasic solvent systems indicated suitable partition coefficients (K values) with n-hexane/ethanol/water (12:8:1, v/v/v) and n-hexane/benzotrifluoride/acetonitrile (20:7:13, v/v/v). After initial tests with conventional CCC, the application of CCC in heart-cut recycling mode provided 4,4-dimethyl-substituted sterols with purities of 99 % (dihydrolanosterol) and 95 % (lanosterol).


Assuntos
Distribuição Contracorrente , Hexanos , Acetonitrilas , Animais , Colesterol , Distribuição Contracorrente/métodos , Etanol , Álcoois Graxos , Lanolina , Lanosterol , Óleos Vegetais , Solventes , Esteróis , Ureia , Água
3.
Dermatitis ; 33(3): 193-199, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35481824

RESUMO

BACKGROUND: Lanolin is an important cause of allergic contact dermatitis. OBJECTIVES: The aims of this study were to describe the epidemiology of lanolin allergy and to assess trends in patch test reactions to lanolin over time. METHODS: This study used a retrospective analysis of patients patch tested with lanolin alcohol 30% or Amerchol L-101 50% in petrolatum by the North American Contact Dermatitis Group between 2001 and 2018 (n = 43,691). RESULTS: Overall, 1431 (3.3%) had a positive reaction, and 1238 (2.8%) were currently relevant. Prevalence of lanolin allergy was 4.63% between 2011 and 2018 (P < 0.0001). Most lanolin-allergic patients had + (52%) reactions; 18%, and 6% had ++ and +++ reactions, respectively. Common primary anatomic sites of dermatitis were the hands (20.7%), scattered/generalized distribution (19.6%), and face (17.0%). Allergic reactions to lanolin were more common in children (4.5%) than in adults (3.2%, P = 0.0018). Compared with nonallergic patients, lanolin-allergic patients were more likely to have history of eczema or hay fever, male sex, older than 40 years, or Black race (P < 0.05). Common lanolin sources were personal care products and drugs/medications. Only 2.24% of the positive reactions were linked to occupation. CONCLUSIONS: Lanolin sensitivity was common. Reactions were often clinically relevant and linked to personal care products and medications.


Assuntos
Cosméticos , Dermatite Alérgica de Contato , Adulto , Alérgenos/efeitos adversos , Criança , Cosméticos/efeitos adversos , Dermatite Alérgica de Contato/epidemiologia , Dermatite Alérgica de Contato/etiologia , Humanos , Lanolina/efeitos adversos , Masculino , América do Norte/epidemiologia , Testes do Emplastro/efeitos adversos , Estudos Retrospectivos
4.
J Biomater Sci Polym Ed ; 33(12): 1511-1530, 2022 08.
Artigo em Inglês | MEDLINE | ID: mdl-35451917

RESUMO

Wound healing is a complex biological process. In this context, hyaluronic acid (HA) plays an important role in all phases of wound healing, from inflammation to the remodelling process. Nevertheless, its presence in adults decreases by 50% compared to newborns, which drastically reduces tissue regeneration. In this sense, this work presented a new method of extracting HA from chicken combs, as well as the development and in vivo evaluation of an ointment composed of vaseline, lanolin and HA 1% (w/w) for wound healing. The rheological analysis showed that the ointment containing HA has a viscoelastic behaviour. The in vivo test showed on the 7th day that the group treated with the ointment containing HA had a wound area of 0.07 cm2 against 0.09 cm2 of the ointment without HA (vaseline, and lanolin). On the other hand, the groups treated with the HA ointment had a higher mean percentage of collagen and better healing on the 14th day. The results of this paper indicate that the new method used to obtain HA is feasible, low-cost, and easy to obtain. Furthermore, the HA containing ointment improved wound healing. Therefore, the obtained ointment has great potential for use as an effective biomaterial in wound healing.


Assuntos
Ácido Hialurônico , Lanolina , Pomadas , Vaselina , Cicatrização
5.
Contact Dermatitis ; 87(1): 40-52, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35184302

RESUMO

BACKGROUND: Diagnosis of contact allergy (CA) to Amerchol L-101 (AL-101), a marker for lanolin allergy, is problematic. Positive patch test reactions are frequently doubtful or weakly positive and difficult to associate with clinical relevance. OBJECTIVE: To gain further insight on the allergic or irritant nature of skin reactions induced by AL-101 patch test. METHODS: We re-tested in a dose-response fashion, 10 subjects with AL-101 CA and performed comprehensive transcriptomic analysis (gene arrays, quantitative real-time polymerase chain reaction [qRT-PCR]) of samples of their skin reactions. RESULTS: Eight of the 10 CA subjects reacted positively upon re-test, whereas two did not react. Most of AL-101 positive patch tests expressed an allergy signature with strong activation of gene modules associated with adaptive immunity and downregulation of cornification pathway genes. In addition, the breadth of gene modulation correlated with the magnitude of patch test reactions and the concentration of AL-101 applied. However, we observed that some of the positive patch test reactions to AL-101 expressed no/few allergy biomarkers, suggesting the induction of an irritant skin inflammation in these samples. CONCLUSIONS: This study confirms that AL-101 is an allergen that can cause both contact allergy and contact irritation. Our results also highlight that molecular profiling might help to strengthen clinical diagnosis.


Assuntos
Dermatite Alérgica de Contato , Alérgenos/efeitos adversos , Dermatite Alérgica de Contato/etiologia , Dermatite Alérgica de Contato/genética , Humanos , Irritantes/efeitos adversos , Lanolina , Testes do Emplastro/métodos
6.
Dermatitis ; 33(2): 135-143, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-34115664

RESUMO

BACKGROUND/OBJECTIVE: Both active and inactive ingredients in topical ophthalmic agents may cause allergic contact dermatitis. Here, we examined ingredients in prescription topical ophthalmic medications available in the United States. METHODS: A comprehensive list of topical ophthalmic medications was generated using AccessPharmacy. Categories included antiglaucoma, antibiotic, antibiotic/corticosteroid, corticosteroid, antiviral, antifungal, mydriatic, and miotic agents. For each formulation, ingredients were investigated using the National Institutes of Health US National Library of Medicine database and/or manufacturer websites. Counts and proportions were calculated for inactive ingredients, including those in the American Contact Dermatitis Society (ACDS) Core 90 Allergen Series. RESULTS: Two hundred sixty-four unique prescription ophthalmic medications met the inclusion criteria. The most common ACDS Core 90 allergen/cross-reactor inactive ingredient was benzalkonium chloride (68.1%, 180/264), followed by sorbates (11.7%, 31/264), parabens (6.8%, 18/264), sodium metabisulfite (3.8%, 10/264), propylene glycol (3.0%, 8/264), and lanolin (3.0%, 8/264). Approximately 21% (20.8%, 55/264) of products had no ACDS Core 90 allergens/cross-reactor inactive ingredients. The most common ACDS Core 90 allergen/cross-reactor active ingredients were aminoglycoside antibiotics, bacitracin/polymyxin B, and corticosteroids. Important non-ACDS Core 90 allergens included inactive ingredients, such as EDTA 28.0% and thimerosal 2.7%, as well as active ingredients, especially ß-blockers. CONCLUSIONS: Benzalkonium chloride, sodium metabisulfite, propylene glycol, and lanolin were common inactive ingredient allergens. Most ophthalmic categories had low allergen formulations available for patients with contact allergy.


Assuntos
Alérgenos , Dermatite Alérgica de Contato , Hipersensibilidade a Drogas , Alérgenos/efeitos adversos , Dermatite Alérgica de Contato/etiologia , Humanos , Lanolina/efeitos adversos , Oftalmologia , Testes do Emplastro , Prescrições , Propilenoglicol/efeitos adversos , Sulfitos/efeitos adversos , Estados Unidos
7.
J Photochem Photobiol B ; 222: 112264, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34320457

RESUMO

Most modern sunscreens contain physical filters, which scatter the sunlight, increasing the photons' pathway in the upper stratum corneum. This effect can lead to a better efficacy of the UV filters and improve the diffuse reflection. However, the addition of nanosized inorganic UV filters reduces the antioxidant capacity of sunscreen formulations. Two cream formulations (F1, F2) which differ in the ingredient PEG75 Lanolin (F2), have been characterized for their radical protection factor (RPF) and their optical properties in vitro using electron paramagnetic resonance (EPR) spectroscopy and UV/VIS spectrometry. The RPF for PEG-75 Lanolin was also determined. Furthermore, their radical protection properties were analyzed on porcine skin ex vivo after visible light irradiation by EPR. The structure of each formulation in the skin surface was determined by reflectance confocal microscopy in vivo. The addition of lanolin increased the reflectance and reduced the transmittance for visible light, improving the scattering drastically. Besides, the antioxidant capacity was also increased for F2, something unpublished until now. F1 presented a lower scattering provided by starches. The sunscreens showed high scattering properties and antioxidant capacity, especially for F2, which presented the lowest radical formation in the skin model. These results are consistent with the RPF measurements where F2 has a higher RPF value (193 ± 3 × 1014 radicals/mg) than F1 (155 ± 4 × 1014 radicals/mg) and for PEG-75 Lanolin (37 ± 1 × 1014 radicals/mg). The combination of starches and PEG-75 Lanolin is the first solution to provide both, light scattering and antioxidant capacity, in sunscreens.


Assuntos
Antioxidantes/química , Lanolina/química , Luz , Amido/química , Protetores Solares/química , Animais , Composição de Medicamentos , Espectroscopia de Ressonância de Spin Eletrônica , Pele/efeitos dos fármacos , Pele/efeitos da radiação , Fator de Proteção Solar , Protetores Solares/farmacologia , Suínos
8.
J Drugs Dermatol ; 20(4): 461-465, 2021 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-33852250

RESUMO

BACKGROUND: Skin rejuvenation can be achieved with minimally invasive treatments using energy-based devices that feature reduced side effects and downtime. Post-treatment care is key to minimize any potential side effects and skin reactions such as erythema, dryness, or dyschromia. OBJECTIVE: The objective of this study was to evaluate the efficacy and patient satisfaction of a novel carboxytherapy gel mask compared with petroleum-based lanolin-containing ointment to accelerate wound healing facial post-nanofractional radiofrequency treatment. METHODS AND MATERIALS: Ten subjects were enrolled in this pilot, prospective, randomized, single-blind study and randomized into two arms. One arm received one nanofractional radiofrequency treatment with ointment right after and four consecutive days of ointment applications twice a day, while the second arm followed this regimen with a carboxytherapy gel mask application right after and four consecutive days after treatment. Investigator, safety, and patient assessments were conducted at 24 hours and one-week post treatment. Safety was monitored throughout. The primary endpoint was defined as the degree of investigator global assessment (IGA) in photodamage, pigmentation, and wrinkles using standardized photographs. Secondary endpoints included investigator-rated degree of erythema, edema, crusting, exudation, percentage healing, improvement of skin quality, and patient satisfaction. RESULTS: Nine patients completed the study. There was improvement of one degree in IGA for photodamage, pigmentation and wrinkles in all patients using the carboxytherapy gel mask at the one-week follow up. Blinded investigator ratings showed significant improvement of dryness, erythema, edema, crusting, and percentage healing at the 24-hour follow up, with all patients remaining the same a week post treatment. All patients in the carboxytherapy group were satisfied with the treatment and had no adverse effects. Three patients in the petroleum-based lanolin-containing group experienced mild edema and acne breakout that resolved two weeks after treatment. CONCLUSION: Carboxytherapy delivered via a gel mask after skin rejuvenation procedures is a safe and effective strategy to improve clinical outcomes and reduce post-treatment side effects. J Drugs Dermatol. 20(4):461-465. doi:10.36849/JDD.5856.


Assuntos
Dióxido de Carbono/administração & dosagem , Técnicas Cosméticas/efeitos adversos , Eritema/tratamento farmacológico , Terapia por Radiofrequência/efeitos adversos , Rejuvenescimento , Administração Cutânea , Adulto , Eritema/diagnóstico , Eritema/etiologia , Face , Feminino , Géis , Humanos , Lanolina/administração & dosagem , Pessoa de Meia-Idade , Pomadas/administração & dosagem , Pomadas/química , Satisfação do Paciente , Petróleo , Fotografação , Projetos Piloto , Estudos Prospectivos , Hipofracionamento da Dose de Radiação , Terapia por Radiofrequência/métodos , Índice de Gravidade de Doença , Método Simples-Cego , Pele/diagnóstico por imagem , Pele/efeitos dos fármacos , Pele/efeitos da radiação , Envelhecimento da Pele/efeitos da radiação , Resultado do Tratamento
9.
Contact Dermatitis ; 84(6): 431-438, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33350482

RESUMO

BACKGROUND: Allergic contact dermatitis (ACD) from topical medication often occurs in occluded areas, for example, with wound treatment, but also in certain body locations, such as the anogenital area. OBJECTIVES: To investigate the demographics and specific lesion location of patients with ACD from topical drugs applied onto the (peri)anal/genital area, and to identify the respective causal topical pharmaceutical products and ingredients involved. METHODS: From January 2000 to December 10, 2018, 532 patients were tested with the baseline series, sometimes with additional series, and the topical medication used along with the ingredients. The relevant data were extracted from our electronic databases developed in-house. RESULTS: Forty-four patients (9%) out of 473 patients suffering from lesions in the (peri)anal/genital area had positive patch test results to topical drug preparations and/or their ingredients, sometimes in association with cosmetics for intimate hygiene. The most frequent sensitizing active principles were local anaesthetics and corticosteroids, while wool alcohols and to a minor extent benzoic acid were the most frequent culprits among the vehicle components and preservative agents, respectively. CONCLUSIONS: The local conditions (eg, occlusion, sweating, moist) in the anogenital area may favour skin sensitization to topical medication used to treat various skin diseases.


Assuntos
Corticosteroides/efeitos adversos , Anestésicos Locais/efeitos adversos , Doenças do Ânus/induzido quimicamente , Cosméticos/efeitos adversos , Dermatite Alérgica de Contato/etiologia , Doenças dos Genitais Femininos/induzido quimicamente , Doenças dos Genitais Masculinos/induzido quimicamente , Adolescente , Adulto , Idoso , Ácido Benzoico/efeitos adversos , Criança , Pré-Escolar , Feminino , Humanos , Higiene , Lactente , Lanolina/efeitos adversos , Masculino , Pessoa de Meia-Idade , Conservantes Farmacêuticos/efeitos adversos , Estudos Retrospectivos , Adulto Jovem
10.
Contact Dermatitis ; 84(1): 41-49, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32844454

RESUMO

BACKGROUND: Lanolin is often included when patch testing for common contact allergens. The clinical relevance of a positive patch test reaction to lanolin markers is, however, still a subject for debate. OBJECTIVES: To evaluate Amerchol L101 as a marker of lanolin allergy and investigate the clinical impact of lanolin-containing moisturizers on healthy and damaged skin using the repeated open application test (ROAT). METHODS: Twelve test subjects and 14 controls were patch tested with Amerchol L 101 and additional lanolin markers. Subsequently, a blinded ROAT was performed on the arms of the study participants for 4 weeks. Each participant applied a lanolin-free cream base and two different lanolin-containing test creams twice daily on one arm with intact skin and on the other arm with irritant dermatitis, induced by sodium lauryl sulfate (SLS). RESULTS: Eleven test subjects (92%) had positive patch test reactions to Amerchol L 101 when retested and one test subject (8%) had a doubtful reaction. None of the study participants had any skin reactions to the ROAT on intact skin and all participants healed during the ROAT on damaged skin. CONCLUSIONS: Lanolin-containing emollients do not cause or worsen existing dermatitis when performing ROAT in volunteers patch test positive to Amerchol L101.


Assuntos
Dermatite Alérgica de Contato/diagnóstico , Dermatite Alérgica de Contato/etiologia , Lanolina/análogos & derivados , Testes do Emplastro/métodos , Creme para a Pele/efeitos adversos , Estudos de Casos e Controles , Dermatite Irritante/diagnóstico , Dermatite Irritante/etiologia , Método Duplo-Cego , Humanos , Lanolina/efeitos adversos , Creme para a Pele/química
11.
Enferm Clin (Engl Ed) ; 31(2): 82-90, 2021.
Artigo em Inglês, Espanhol | MEDLINE | ID: mdl-33277168

RESUMO

OBJECTIVE: To investigate the effect of lanolin on nipple pain and trauma in breastfeeding after application of a health education. METHOD: Randomized controlled clinical trial, with two arms, open, with 66 participants during prenatal care in the primary health care network in Goiania - Goias, Brazil. Participants were randomized (1:1) using computer generated numbers in both experimental group (EG) and control group (CG). The EG received lanolin and health education on breastfeeding at two different times with clinical demonstration using cloth didactic breast and illustrative album as the intervention, while the CG received standard health education. Health education was carried out by the same researchers in both groups. Measurement of pain, nipple trauma, and breastfeeding technique occurred on postpartum day eight. The analysis included descriptive statistics and inferential analysis by means chi-square or Fisher test, and Student's t-test, significance level set at 0.05. RESULTS: A majority of the participants experienced no nipple trauma (59.1%) in both groups, and 60.6% of women experienced pain. In both groups, women showed favorable breastfeeding behaviors, except in the condition of the breasts. There were no significant differences between groups in pain prevention (p=0.61), nipple lesions (p=0.21), and breastfeeding technique (p>0.05). CONCLUSION: It is not clear whether the intervention, lanolin combined with health education, has a positive effect on the prevention of nipple pain and trauma. Further research is needed to elucidate this question. Registration number: RBR-7tvhq8. Registry website: http://www.ensaiosclinicos.gov.br/.


Assuntos
Lanolina , Mamilos , Brasil , Aleitamento Materno , Feminino , Educação em Saúde , Humanos , Gravidez
12.
J Oleo Sci ; 69(10): 1317-1329, 2020 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-32908094

RESUMO

This study was aimed to prepare oleogels of whale spermaceti wax (WsWO) and lanolin wax (LnWO), and to compare them with well-known animal wax oleogels of shellac (ShWO) and beeswax (BsWO). WsWO, ShWO and BsWO were prepared at 5% (w/w) organogelator addition level, while LnWO was necessarily prepared at its minimum gelling concentration (C*) of 30% (w/w) addition level. All oleogels were posed high oil binding capacity and thermal reversibility. Melting peak temperatures were ordered as ShWO > BsWO > WsWO > LnWO by calorimetry. X-ray diffraction patterns indicated the presence of both ß´and ß type polymorphs, together with needle-like crystal morphology. Rheological analyses indicated that the stiffness of the gels were ordered as BsWO > WsWO > LnWO > ShWO. All showed good thixotropy, and thermal stability until 40°C (ShWO until 80°C). Finally, the sensory descriptive analysis indicated that LnWO had distinct negative sheep odor, but WsWO was quite similar to BsWO. Overall, LnWO determined to be not proper for food applications, but WsWO was shown to be a suitable oleogel for food applications.


Assuntos
Qualidade dos Alimentos , Alimentos , Odorantes/análise , Adulto , Animais , Fenômenos Químicos , Cristalização , Feminino , Humanos , Lanolina , Masculino , Pessoa de Meia-Idade , Compostos Orgânicos/química , Resinas Vegetais , Reologia , Temperatura , Ceras , Baleias , Adulto Jovem
13.
J Wound Care ; 29(2): 94-99, 2020 Feb 02.
Artigo em Inglês | MEDLINE | ID: mdl-32058849

RESUMO

OBJECTIVE: Children are at high risk of injuries and wounds. The application of medical grade honey is a promising approach to improving the healing of wounds of various origin and severity. However, the use of medical grade honey in young paediatric patients remains limited. The aim of this study is to show the safety, efficacy and usefulness of medical grade honey in abdominal wounds, of different causes, in paediatric patients. METHOD: This was a prospective, observational case series evaluating five young infants with abdominal wounds at the General Hospital in Thessaloniki. All wounds were treated in the same manner with daily medical grade honey applied to the wound area and closely monitored. RESULTS: All treated wounds rapidly presented granulation tissue formation and underwent re-epithelialisation. Peripheral oedema and inflammation decreased upon initial application. Necrotic tissue was effectively debrided when present. Slough was removed and no signs of infection were detected, irrespective of initial wound presentations. Scar formation was minimal and the full range of motion was preserved in all cases. CONCLUSION: Based on this case study, medical grade honey is safe and effective in treating different abdominal wounds, including infected or dehisced wounds as well as burns. The easy application and broad applicability make medical grade honey recommendable as a first-line treatment in paediatric patients.


Assuntos
Traumatismos Abdominais/terapia , Apiterapia/métodos , Queimaduras/terapia , Mel , Reepitelização , Deiscência da Ferida Operatória/terapia , Infecção da Ferida Cirúrgica/terapia , Apendicectomia , Apendicite/cirurgia , Ácido Ascórbico/uso terapêutico , Infecções por Bacteroides/terapia , Queimaduras Químicas/terapia , Criança , Pré-Escolar , Fármacos Dermatológicos/uso terapêutico , Farmacorresistência Bacteriana Múltipla , Edema , Feminino , Gastrostomia , Grécia , Humanos , Lactente , Recém-Nascido , Inflamação , Infecções por Klebsiella/terapia , Lanolina/uso terapêutico , Masculino , Neuroblastoma/cirurgia , Óleos Voláteis/uso terapêutico , Pomadas , Estudos Prospectivos , Neoplasias Retroperitoneais/cirurgia , Vitamina E/uso terapêutico , Vitaminas/uso terapêutico , Óxido de Zinco/uso terapêutico
14.
Biomolecules ; 10(2)2020 02 11.
Artigo em Inglês | MEDLINE | ID: mdl-32054132

RESUMO

Anthocyanin contributes to the coloration of pear fruit and enhances plant defenses. Members of the ethylene response factor (ERF) family play vital roles in hormone and stress signaling and are involved in anthocyanin biosynthesis. Here, PbERF22 was identified from the lanolin-induced red fruit of 'Zaosu' pear (Pyrus bretschneideri Rehd.) using a comparative transcriptome analysis. Its expression level was up- and down-regulated by methyl jasmonate and 1-methylcyclopropene plus lanolin treatments, respectively, which indicated that PbERF22 responded to the jasmonate- and ethylene-signaling pathways. In addition, transiently overexpressed PbERF22 induced anthocyanin biosynthesis in 'Zaosu' fruit, and a quantitative PCR analysis further confirmed that PbERF22 facilitated the expression of anthocyanin biosynthetic structural and regulatory genes. Moreover, a dual luciferase assay showed that PbERF22 enhanced the activation effects of PbMYB10 and PbMYB10b on the PbUFGT promoter. Therefore, PbERF22 responses to jasmonate and ethylene signals and regulates anthocyanin biosynthesis. This provides a new perspective on the correlation between jasmonate-ethylene crosstalk and anthocyanin biosynthesis.


Assuntos
Acetatos/metabolismo , Antocianinas/biossíntese , Ciclopentanos/metabolismo , Proteínas de Ligação a DNA/metabolismo , Etilenos/metabolismo , Lanolina/farmacologia , Oxilipinas/metabolismo , Proteínas de Plantas/metabolismo , Antocianinas/genética , Cor , DNA de Plantas/metabolismo , Proteínas de Ligação a DNA/genética , Frutas/efeitos dos fármacos , Frutas/genética , Frutas/metabolismo , Regulação da Expressão Gênica de Plantas , Genes Reguladores/genética , Reguladores de Crescimento de Plantas/metabolismo , Proteínas de Plantas/genética , Regiões Promotoras Genéticas/genética , Pyrus/genética , Pyrus/metabolismo , Transcriptoma/efeitos dos fármacos , Regulação para Cima/efeitos dos fármacos
15.
Dermatitis ; 30(6): 363-370, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31724990

RESUMO

BACKGROUND: Both surgical personnel and patients undergoing procedures are exposed regularly to different antiseptic chemicals in various forms. Little is known about the ingredients in these antiseptics and the risk these products may provoke allergic contact dermatitis. OBJECTIVE: The aim of the study was to identify and characterize common allergens in surgical scrubs and patient surgical cleansers that health care workers and surgical patients may encounter in the perioperative period. METHODS: DailyMed website was searched using numerous terms for surgical disinfectants. Products used for health care worker handwashing/scrubbing or patient surgical cleansing/disinfecting were included. Each product's ingredients were recorded; those found on the 2017 American Contact Dermatitis Society (ACDS) Core Allergen Series were noted from each product. CONCLUSIONS: A total of 1940 products were identified, of which 267 were included in the analysis. A total of 66.3% contained iodine, 25.8% contained chlorhexidine digluconate, and 2.6% contained chloroxylenol. Within the group analyzed, 1586 ingredients were identified. Of these, 241 were ACDS Core Series allergens. Most products contained a single ACDS allergen. There were significant differences in allergens based on product type and active ingredient, with iodine-containing products having the fewest number of allergens. The most common ACDS allergens found were cocamide diethanolamide (22.5%), fragrance (21.7%), lanolin (19.5%), propylene glycol (6.7%), alkyl glucosides (6.0%), and sorbic acid derivatives (5.6%).


Assuntos
Alérgenos/efeitos adversos , Anti-Infecciosos Locais/efeitos adversos , Dermatite Alérgica de Contato/etiologia , Higienizadores de Mão/efeitos adversos , Procedimentos Cirúrgicos Operatórios , Anti-Infecciosos Locais/química , Clorexidina/efeitos adversos , Clorexidina/análogos & derivados , Dermatite de Contato/etiologia , Dermatite Ocupacional/etiologia , Desinfecção das Mãos , Higienizadores de Mão/química , Pessoal de Saúde , Humanos , Lanolina/efeitos adversos , Salas Cirúrgicas , Perfumes/efeitos adversos , Povidona-Iodo/efeitos adversos , Propilenoglicol/efeitos adversos , Xilenos/efeitos adversos
16.
J Drugs Dermatol ; 18(10): 1002-1004, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31584778

RESUMO

Lanolin alcohol is a high cholesterol containing naturally derived material used as a skin protectant in wound healing petrolatum-based ointments. It is a highly purified fraction of lanolin wool wax that has been identified as a possible cause of allergic contact dermatitis. This 3-center study enrolled 499 subjects who underwent a variety of in-office surgical procedures followed by application of a wound healing ointment containing lanolin alcohol without antibiotics. No allergic contact dermatitis was identified in the 499 subjects who completed the study. The lack of allergic contact dermatitis observed may be due to the proprietary highly purified lanolin alcohol utilized in the study formulation. This is not the lanolin alcohol preparation found on the standard dermatology patch test tray. Not all lanolin alcohols are equal. This is an important consideration when examining the reported incidence of allergic contact dermatitis to lanolin alcohol and the absence of allergic contact dermatitis demonstrated in this research. J Drugs Dermatol. 2019;18(10):1002-1004.


Assuntos
Dermatite Alérgica de Contato/epidemiologia , Lanolina/efeitos adversos , Vaselina/efeitos adversos , Ferida Cirúrgica/tratamento farmacológico , Adolescente , Adulto , Idoso , Estudos de Coortes , Dermatite Alérgica de Contato/etiologia , Feminino , Humanos , Lanolina/administração & dosagem , Masculino , Pessoa de Meia-Idade , Bases para Pomadas , Pomadas , Vaselina/administração & dosagem , Prevalência , Resultado do Tratamento , Cicatrização/efeitos dos fármacos , Adulto Jovem
17.
Complement Ther Med ; 43: 300-305, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30935548

RESUMO

OBJECTIVES: The traditional uses of Portulaca oleracea L. (purslane) with anti-inflammatory and anti-cancer activity as well as anti-oxidants properties were expressed previously. This is a double-blind randomized clinical trial to evaluate the protective effects of purslane cream on the nipple fissure. METHODS: After expressing the goals and methods of the study and obtaining written consent from 86 lactating women with nipple fissure, they were randomly divided into two groups: 43 in purslane cream group and 43 in lanolin ointment group. The score of nipple fissure before the intervention and on the third and eighth day after the study was measured using the Stour scale. RESULTS: The mean score of left and right breast fissures in the group of treatment with lanolin group similar to the group of treatment with purslane cream showed a significant decrease at the third day and eighth day (P=0.001). Mann-Whitney test comparing mean score of the fissure between two groups showed that two groups were homogeneous before the intervention, but there was a significant difference between two groups on the third and eighth days (p < 0.001). The recovery process occurred faster in the group of treatment with purslane cream. CONCLUSION: We showed that the use of purslane cream without any complications could accelerate the repairing of nipple fissure. Based on this clinical trial, purslane cream (2% w/w) can be used as an accelerator for improving the nipple fissure in lactating women.


Assuntos
Lactação/efeitos dos fármacos , Mamilos/efeitos dos fármacos , Pomadas/uso terapêutico , Portulaca/química , Cicatrização/efeitos dos fármacos , Adulto , Método Duplo-Cego , Feminino , Humanos , Lanolina/uso terapêutico
18.
J Sep Sci ; 42(12): 2171-2178, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30950563

RESUMO

Lanosterol is a potential drug for cataracts treatment, which can reverse the aggregation of intracrystalline proteins. The low concentration in lanolin calls for high-performance separation methods. In this study, a counter-current chromatography dual-mode elution method was developed for the first time to separate and purify lanosterol from hexane extract of lanolin after saponification, in which the column was first eluted with the lower phase as mobile phase in head-to-tail mode, followed by the upper phase in the tail-to-head mode. High purity of lanosterol, dihydrolanosterol, and cholesterol can be obtained simultaneously. A solvent system composed of n-heptane/acetonitrile/ethyl acetate (5:5:1, v/v/v) was selected and optimized via partition coefficient determination. Compounds such as 111 mg lanosterol, 84 mg dihydrolanosterol, and 183 mg cholesterol with high purity of 99.77, 95.71, and 91.43%, respectively, analyzed by high-performance liquid chromatography were obtained within 80 min from 700 mg crude extract from 1.78 g lanolin. The method was also used to improve the purity of commercial lanosterol product from 66.97 to above 99%. Counter-current chromatography could serve as a potential and powerful technique for commercial production of highly pure lanosterol.


Assuntos
Colesterol/isolamento & purificação , Lanolina/química , Lanosterol/isolamento & purificação , Colesterol/química , Cromatografia Líquida de Alta Pressão , Distribuição Contracorrente , Lanosterol/química , Conformação Molecular
19.
Microbiol Res ; 219: 49-55, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30642466

RESUMO

Volatile organic compounds (VOCs) released from bacterial species have been reported as plant growth inducers. In this sense, Lactuca sativa was used as model vegetable to prospect the effects of 2-nonanone released by Bacillus sp. BCT9 at cellular and organ structure level, so we present preliminary results about the physiological effects. In this study, 2-day-old L. sativa were exposed to 2-nonanone for 10 days under two delivery systems: 1) 2-nonanone (abrupt delivery) and 2) 2-nonanone + lanolin (controlled delivery). The X-ray elemental microanalysis, scanning electron and confocal laser microscopies techniques were used to evaluate physiological changes "in vivo" conditions. The results indicated that 2-nonanone increased root and shoot length independently of 2-nonanone delivery system after 7 days of exposition. Additionally, 2-nonanone elicited the increase of anthocyanin and not affects chlorophyll content and electrolyte leakage percentage. The abrupt delivery elicited the increase of both length and density of root hair without causing changes in size of cell epidermis, while controlled delivery induced stomatal opening. Besides, 2-nonanone exposition did not modify the composition and distribution of carbon, nitrogen, phosphorus, potassium, and chlorine in the surface of plant tissue. The results suggested that 2-nonanone acts as a bacterial signal molecule to elicit changes related to root development without damaging the external morphology while epidermal cells at leaf level are not affected, suggesting that 2-nonanone can be an important tool to apply to vegetables.


Assuntos
Bacillus/metabolismo , Cetonas/farmacologia , Alface/crescimento & desenvolvimento , Raízes de Plantas/crescimento & desenvolvimento , Brotos de Planta/crescimento & desenvolvimento , Plântula/crescimento & desenvolvimento , Antocianinas/biossíntese , Clorofila/metabolismo , Células Epidérmicas/efeitos dos fármacos , Lanolina/farmacologia , Alface/microbiologia , Microscopia Confocal , Microscopia Eletrônica de Varredura , Folhas de Planta/metabolismo , Compostos Orgânicos Voláteis/metabolismo
20.
Contact Dermatitis ; 80(5): 298-303, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30624788

RESUMO

BACKGROUND: The prevalence of lanolin contact allergy in dermatitis patients varies from 1.2% to 6.9%. Different lanolin derivatives are used in patch testing. OBJECTIVES: To determine which combination of lanolin derivatives is most effective in patch testing for the diagnosis of lanolin contact allergy. METHODS: A retrospective analysis of patients patch tested between 2016 and 2017 was performed. Patients were eligible if they had been tested with lanolin alcohol 30% pet., Amerchol L101 50% pet., and a supplementary series containing other lanolin derivatives. Lanolin alcohol and Amerchol L101 were tested in duplicate. RESULTS: Of 594 patients, 28.6% (95% confidence interval [CI]: 25.1%-32.3%) had a positive patch test reaction to at least one lanolin derivative. Reactions to lanolin alcohol (14.7%, 95%CI: 11.3%-18.2%) and Amerchol L101 (15.0%, 95%CI: 11.5%-18.5%) were common in the routinely tested series. Reactions to other test preparations were significantly less frequent (P < 0.05). The addition of Amerchol L101 to lanolin alcohol significantly increased the number of positive cases (odds ratio 1.79, P < 0.001). CONCLUSIONS: The combination of lanolin alcohol and Amerchol L101 is effective in patch testing for the diagnosis of lanolin contact allergy. Routinely testing with other lanolin derivatives may not be worthwhile, as it detects only a few additional patients.


Assuntos
Dermatite Alérgica de Contato/diagnóstico , Lanolina/efeitos adversos , Testes do Emplastro/métodos , Adulto , Álcoois/efeitos adversos , Dermatite Alérgica de Contato/etiologia , Feminino , Humanos , Lanolina/análogos & derivados , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Adulto Jovem
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