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1.
Chemosphere ; 286(Pt 2): 131707, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34365170

RESUMO

2-Pentanone is an excellent organic solvent and extractant, which is widely used in industrial production. 2-Pentanone is harmful to soil organisms when it enters the soil. However, current studies have not clarified the response of the antioxidant enzyme superoxide dismutase (SOD) to 2-Pentanone and its mechanism. In this study, the response of earthworm antioxidant enzyme SOD to 2-Pentanone and its molecular mechanism was investigated at organism molecular levels. The results showed that the SOD activity of earthworms under 2-Pentanone stress was significantly inhibited, and the inability of superoxide anion radicals (·O2-) to be scavenged in time might be one of the reasons for the increase of lipid peroxidation. Under 2-Pentanone exposure conditions, catalase (CAT), an antioxidant enzyme closely related to SOD, and the total antioxidant capacity (T-AOC) of earthworms were activated to resist oxidative damage. On the other hand, the observation of earthworm microstructure provided evidence of a direct risk of 2-Pentanone on earthworm body wall tissues. Molecular-level assays have shown that 2-pentanone altered the secondary structure of SOD, which further led to the loosening of the SOD backbone structure and the extension of the polypeptide chain. On the other hand, 2-pentanone quenched the endogenous fluorescence of SOD in the form of static quenching and formed the 2-pentanone/SOD complex. Molecular simulation results suggested that 2-pentanone tended to bind on the surface of SOD rather than close to the active site, and it is speculated that the alteration of SOD structure is the key reason for the change in its activity. This study enriches the toxicological data of 2-Pentanone on soil organisms, thus responding to the current concerns about its ecological risk.


Assuntos
Oligoquetos , Poluentes do Solo , Animais , Catalase/metabolismo , Malondialdeído , Oligoquetos/metabolismo , Estresse Oxidativo , Pentanonas , Poluentes do Solo/toxicidade , Superóxido Dismutase/metabolismo
2.
Biol Trace Elem Res ; 200(1): 172-182, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33682074

RESUMO

T-2 toxin is a trichothecene mycotoxin produced by fusarium species, which is mainly prevalent in grain and livestock feed. One of the main effects of this toxin is immunodepression. Previous studies have shown that T-2 toxin can cause damage to immune organs and impaired immune function in animals. However, selenomethionine (SeMet) as an organic selenium source can not only promote the growth and development of the body but also effectively improve the body's immune function. In this study, rabbits were exposed to 0.4-mg/kg T-2 toxin, and abnormal blood routine indicators were found in the rabbits. HE staining also showed obvious lesions in the spleen and thymus tissue structures, accompanied by a large number of bleeding points. In addition, rabbits showed strong oxidative stress and inflammatory response after T-2 toxin action. 0.2 mg/kg, 0.4 mg/kg, and 0.6 mg/kg organic selenium were added to the feed. However, it was found that 0.2 mg/kg selenium can effectively improve the abnormal changes of blood routine and spleen and thymus tissue of rabbits. On the other hand, it can significantly increase the expression of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and total antioxidant capacity (T-AOC) in the spleen and thymus, and downregulate the expression of reactive oxygen species (ROS) and malondialdehyde (MDA). In addition, inflammatory factors interleukin-1 beta (IL-1ß) and interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) in blood were also significantly inhibited; the expression of proliferating cell nuclear antigen (PCNA) in the spleen and thymus was also significantly increased after low-dose selenium treatment. Surprisingly, 0.4 mg/kg and 0.6 mg/kg of selenium did not effectively alleviate the immunotoxic effects caused by T-2 toxin, and cause damage to a certain extent. In summary, our results show that 0.2 mg/kg of SeMet can effectively alleviate the immunotoxicity caused by T-2 toxin. Selenium may protect rabbits from T-2 toxin by improving its antioxidant and anti-inflammatory capabilities.


Assuntos
Selênio , Toxina T-2 , Animais , Antioxidantes/farmacologia , Malondialdeído , Estresse Oxidativo , Coelhos , Selenometionina/metabolismo , Selenometionina/toxicidade , Toxina T-2/toxicidade
3.
Acta Cir Bras ; 36(10): e361005, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34817026

RESUMO

PURPOSE: Reactive oxygen species (ROS), interleukin-1ß (IL-1ß) and tumor necrosis factor-α (TNF-α) have been shown in the pathogenesis of acrylamide neurotoxicity. Hippophae rhamnoides L. extract (HRE) has a cytoprotective effect by stabilizing the production of ROS, IL-1ß and TNF-α. The objective of the article was to investigate the effect of HRE on acrylamide-induced brain damage in rats biochemically and histopathologically. METHODS: To the HRE+acrylamide only (ACR) group (n=6) of the animals, HRE was administered orally at a dose of 50 mg / kg into the stomach by gavage. The same volume of solvent (olive oil) was administered orally to the ACR (n=6) and healthy (HG) (n=6) groups. One hour after HRE administration, acrylamide was given orally at a dose of 20 mg/kg to HRE+ACR and ACR groups in the same way. This procedure was repeated once a day for 30 days. At the end of this period, brain tissues extracted from animals killed with 50 mg/kg thiopental anesthesia were examined biochemically and histopathologically. RESULTS: It has been shown that HRE prevents the increase of malondialdehyde (MDA), myeloperoxidase (MPO), IL-1ß and TNF-α with acrylamide and the decrease of total glutathione (tGSH) and glutathione reductase (GSHRd) levels in brain tissue. CONCLUSIONS: HRE may be useful in the treatment of acrylamide-induced neurotoxicity.


Assuntos
Lesões Encefálicas , Hippophae , Animais , Antioxidantes/farmacologia , Malondialdeído , Estresse Oxidativo , Extratos Vegetais/farmacologia , Extratos Vegetais/uso terapêutico , Ratos
4.
Wei Sheng Yan Jiu ; 50(5): 781-787, 2021 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-34749872

RESUMO

OBJECTIVE: To investigate the protective effect of oleanolic acid(OA) on HgCl_2 induced liver injury. METHODS: L02 cells were divided into four groups according to different treatment, control group(Con), oleanolic acid group(OA, 10 µmol/L), HgCl_2 group(HgCl_2, 40 µmol/L) and oleanolic acid + HgCl_2 group(OA + HgCl_2). Cells of control group were given serum-free medium, cells of OA group were pretreated with OA solution for 8 hours, cells of HgCl_2 group were exposed to HgCl_2 solution for 6 hours, cells of OA + HgCl_2 group were pretreated with OA solution for 8 hours, and then exposed to HgCl_2 solution for 6 hours. MTT assay was used to detect cell viability. Laser confocal scanning was used to detect JC-1 probe fluorescence intensity to determine mitochondrial membrane potential. DCFH-DA fluorescence probe combined with flow cytometry was used to detect reactive oxygen species(ROS) level. Annexin V/PI double staining method combined with flow cytometry was used to determine cell apoptosis rate. Catalase(CAT), total superoxide dismutase(T-SOD), glutathione(GSH), malondialdehyde(MDA), Caspase 3 and Caspase 9 kits combined with enzyme labeled instrument were used to determine their activity or content respectively. RESULTS: Compared with the control group, 40 µmol/L HgCl_2 could significantly reduce cell viability, the level was 0.52±0.03(P<0.05), OA pretreatment could significantly inhibit the decrease of cell viability induced by HgCl_2, the level was 0.86±0.05(P<0.05). The result of mitochondrial membrane potential detection showed that cell exposed to 40 µmol/L HgCl_2 significantly reduced the intensity of red fluorescence, and the ratio of red to green fluorescence was 0.23±0.02(P<0.05). OA pretreatment significantly increased red fluorescence, and the ratio of red fluorescence to green fluorescence was 1.32±0.08, which was significantly higher than that of HgCl_2(P<0.05). After exposure to 40 µmol/L HgCl_2, the relative fluorescence intensity of ROS was 1.21±0.07, the apoptosis rate was about 8%, the activity levels of Casepase 3 and Casepase 9 were 3.11±0.20 and 2.94±0.17, respectively, which were all significantly higher than those in the control group(P<0.05). OA pretreatment could significantly alleviate the changes of the above indexes, and the difference was statistically significant compared with HgCl_2 group(P<0.05). The level of T-SOD in HgCl_2 group was(7.68±0.39)U/mL, which was significantly lower than that in control group(P<0.05). Compared to the control group, the level of MDA was significantly increased to(4.99±0.26)nmol/mg(P<0.05). OA pretreatment significantly increased level of T-SOD and decreased the level of MDA, the levels were(13.97±0.71)U/mL and(3.01±0.17)nmol/mg, respectively(P<0.05). CONCLUSION: A certain concentration of HgCl_2 can induce hepatocyte damage. OA pretreatment may reduce cell damage by improving oxidative stress.


Assuntos
Ácido Oleanólico , Apoptose , Hepatócitos , Malondialdeído , Ácido Oleanólico/farmacologia , Estresse Oxidativo , Espécies Reativas de Oxigênio
5.
J Insect Sci ; 21(5)2021 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-34718645

RESUMO

Radiation is considered as a promising insect pest control strategy for minimizing postharvest yield losses. Among various techniques, irradiation is a method of choice as it induces lethal biochemical or molecular changes that cause a downstream cascade of abrupt physiological abnormalities at the cellular level. In this study, we evaluated the effect of 60Co-γ radiation on various developmental stages of Zeugodacus cucurbitae Coquillett and subsequent carry-over effects on the progeny. For this purpose, we treated eggs with 30- and 50-Gy radiation doses of 60Co-γ. We found that radiation significantly affected cellular antioxidants, insect morphology, and gene expression profiles. Our results indicate that in response to various doses of irradiation reactive oxygen species, catalase, peroxidase, and superoxide dismutase activities were increased along with a significant increase in the malondialdehyde (MDA) content. We observed higher mortality rates during the pupal stage of the insects that hatched from irradiated eggs (50 Gy). Furthermore, the life span of the adults was reduced in response to 50 Gy radiation. The negative effects carried over to the next generation were marked by significantly lower fecundity in the F1 generation of the irradiation groups as compared to control. The radiation induced morphological abnormalities at the pupal, as well as the adult, stages. Furthermore, variations in the gene expression following irradiation are discussed. Taken together, our results signify the utility of 60Co-γ radiation for fruit fly postharvest management.


Assuntos
Apoptose/efeitos da radiação , Raios gama , Expressão Gênica/efeitos da radiação , Tephritidae/efeitos da radiação , Animais , Antioxidantes/metabolismo , Antioxidantes/efeitos da radiação , Apoptose/genética , Catalase/metabolismo , Catalase/efeitos da radiação , Radioisótopos de Cobalto/farmacologia , Controle de Insetos/métodos , Proteínas de Insetos/metabolismo , Proteínas de Insetos/efeitos da radiação , Larva/genética , Larva/metabolismo , Larva/fisiologia , Larva/efeitos da radiação , Longevidade/efeitos da radiação , Malondialdeído/metabolismo , Malondialdeído/efeitos da radiação , Peroxidase/metabolismo , Peroxidase/efeitos da radiação , Controle de Pragas/métodos , Pupa/genética , Pupa/metabolismo , Pupa/fisiologia , Pupa/efeitos da radiação , Espécies Reativas de Oxigênio/metabolismo , Espécies Reativas de Oxigênio/efeitos da radiação , Tephritidae/genética , Tephritidae/metabolismo , Tephritidae/fisiologia
6.
Nutrients ; 13(10)2021 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-34684653

RESUMO

Nonalcoholic fatty liver disease (NAFLD) shows extensive liver cell destruction with lipid accumulation, which is frequently accompanied by metabolic comorbidities and increases mortality. This study aimed to investigate the effects of coffeeberry (CB) on regulating the redox status, the CaMKII/CREB/BDNF pathway, autophagy, and apoptosis signaling by a NAFLD rodent model senescence-accelerated mice prone 8 (SAMP8). Three-month-old male SAMP8 mice were divided into a control group and three CB groups (50, 100, and 200 mg/kg BW), and fed for 12 weeks. The results show that CB reduced hepatic malondialdehyde and carbonyl protein levels. CB significantly enhanced Ca2+/calmodulin-dependent protein kinase II (CaMKII) and brain-derived neurotrophic factor (BDNF) and reduced the phospho-cAMP response element-binding protein (p-CREB)/CREB ratio. In addition, CB increased the silent information regulator T1 level, promoted Beclin 1 and microtubule-associated protein light chain 3 II expressions, and reduced phosphorylated mammalian target of rapamycin and its downstream p-p70s6k levels. CB also inhibited the expressions of apoptosis-related factors poly (ADP-ribose) polymerase-1 and the apoptosis-inducing factor. In conclusion, CB might protect the liver by reducing oxidative stress, activating the CaMKII/CREB/BDNF pathway, and improving autophagic and apoptotic expressions in a dose-dependent manner.


Assuntos
Apoptose , Autofagia , Fator Neurotrófico Derivado do Encéfalo/metabolismo , Proteína Quinase Tipo 2 Dependente de Cálcio-Calmodulina/metabolismo , Café/química , Proteína de Ligação ao Elemento de Resposta ao AMP Cíclico/metabolismo , Hepatopatia Gordurosa não Alcoólica/metabolismo , Transdução de Sinais , Animais , Caspases/metabolismo , Comportamento Alimentar , Fígado/patologia , Masculino , Malondialdeído/metabolismo , Camundongos , Hepatopatia Gordurosa não Alcoólica/patologia , Tamanho do Órgão , Oxirredução , Carbonilação Proteica , Ganho de Peso
7.
FASEB J ; 35(11): e21854, 2021 11.
Artigo em Inglês | MEDLINE | ID: mdl-34597422

RESUMO

Ammonia is one of the major metabolites produced by intestinal microorganisms; however, its role in intestinal homeostasis is poorly understood. The present study investigated the regulation of intestinal tight junction (TJ) proteins by ammonia and the underlying mechanisms in human intestinal Caco-2 cells. Ammonia (15, 30, and 60 mM) increased the permeability of the cells in a dose-dependent manner, as indicated by reduced transepithelial electrical resistance and increased dextran flux. Immunoblot and immunofluorescence analyses revealed that the ammonia-induced increase in TJ permeability reduced the membrane localization of TJ proteins such as zonula occludens (ZO)1, ZO2, occludin, claudin-1, and claudin-3. DNA microarray analysis identified a biological pathway "response to reactive oxygen species" enriched by ammonia treatment, indicating the induction of oxidative stress in the cells. Ammonia treatment also increased the malondialdehyde content and decreased the ratio of reduced to oxidized glutathione. Meanwhile, ammonia treatment-induced mitochondrial dysfunction, as indicated by the downregulation of genes associated with the electron transport chain, reduction of the cellular ATP, NADH, and tricarboxylic acid cycle intermediate content, and suppression of the mitochondrial membrane potential. In contrast, N-acetyl cysteine reversed the ammonia-induced impairment of TJ permeability and structure without affecting the mitochondrial parameters. Collectively, ammonia impaired the TJ barrier by increasing oxidative stress in Caco-2 cells. A mitochondrial dysfunction is possibly an event preceding ammonia-induced oxidative stress. The findings of this study could potentially improve our understanding of the interplay between intestinal microorganisms and their hosts.


Assuntos
Amônia/farmacologia , Mitocôndrias/efeitos dos fármacos , Mitocôndrias/metabolismo , Transdução de Sinais/efeitos dos fármacos , Proteínas de Junções Íntimas/metabolismo , Junções Íntimas/efeitos dos fármacos , Junções Íntimas/metabolismo , Trifosfato de Adenosina/metabolismo , Células CACO-2 , Glutationa/metabolismo , Humanos , Interleucina-8/biossíntese , Mucosa Intestinal/metabolismo , Malondialdeído/metabolismo , Potencial da Membrana Mitocondrial/efeitos dos fármacos , NADP/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Permeabilidade/efeitos dos fármacos , Insuficiência Renal Crônica/metabolismo
8.
Rom J Morphol Embryol ; 62(1): 179-190, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34609420

RESUMO

Testicular torsion is a urological problem that causes subfertility and testicular damage in males. Testis torsion and detorsion lead to ischemia-reperfusion (IR) injury in the testis. Testicular IR injury causes the increase of reactive oxygen species (ROS), oxidative stress (OS) and germ cell-specific apoptosis. In this study, we aimed to investigate whether Carvacrol has a protective effect on testicular IR injury and its effects on Kir6.2 channels, which is a member of adenosine triphosphate (ATP)-dependent potassium channels. In the study, 2-4 months old 36 albino Wistar rats were used. For experimental testicular IR model, the left testis was rotated counterclockwise at 720° for two hours, and after two hours following torsion, detorsion was performed. Carvacrol was dissolved in 5% Dimethyl Sulfoxide (DMSO) at a dose of 73 mg∕kg and half an hour before detorsion, 0.2 mL was administered intraperitoneally. In testicular tissues, caspase 3 and Kir6.2 immunoexpressions were examined. Serum malondialdehyde (MDA) and testosterone levels were measured. Apoptotic cells and serum MDA levels were significantly decreased and Kir6.2 activation was significantly increased in Carvacrol-administrated IR group. As a result of our study, Carvacrol may activates Kir6.2 channels and inhibits apoptosis and may have a protective effect on testicular IR injury.


Assuntos
Traumatismo por Reperfusão , Torção do Cordão Espermático , Trifosfato de Adenosina , Animais , Apoptose , Cimenos , Humanos , Masculino , Malondialdeído , Potássio , Canais de Potássio , Ratos , Traumatismo por Reperfusão/tratamento farmacológico , Torção do Cordão Espermático/tratamento farmacológico , Testículo
9.
J Neurodev Disord ; 13(1): 50, 2021 10 19.
Artigo em Inglês | MEDLINE | ID: mdl-34666671

RESUMO

BACKGROUND: The pathogenesis of autism spectrum disorder (ASD) remains a medical challenge even in the developed world. Although genetics and epigenetic factors have been variously indicted as major causes of the disorder, development of oxidative stress especially in the formative years of children has equally gained prominence as an etiological basis of the disorder. Oxidative stress is characterized by the production of excessive amounts of free radicals, decreased levels of antioxidants with the attendant imbalance in oxidant/antioxidant ratio. This study was designed to determine the levels of essential metals [magnesium (Mg), zinc (Zn), and copper (Cu)] and toxic metal, lead (Pb), and generation of oxidative stress by their abnormal interaction. METHOD: Twenty-five children clinically diagnosed for ASD according to DSM-IV-TR and 25 neuro-typical (NT) children (controls), (aged 5.96 ± 1.40 years and 6.18 ± 2.59 years respectively) were recruited for this study. Essential and toxic metals were analyzed using induction-coupled plasma-mass spectrometry (ICP-MS); oxidative stress markers [malondialdehyde (MDA), total plasma peroxidase (TPP), and total antioxidant capacity (TAC)] were determined using appropriate biochemical methods. Oxidative stress index (OSI) was calculated. RESULTS: The levels of TPP and TAC were significantly reduced while MDA was higher in ASD compared to NT. Although OSI was higher in ASD, the difference was not significant. Pb (lead) concentration was significantly increased while Mg, Zn, and Cu levels were reduced significantly in ASD compared to NT. A significant negative correlation between Mg and OSI (r = - 0.438; p = 0.029) was observed in NT. CONCLUSION: Reduction in Zn and Mg levels with a concurrent increase in Pb in children with ASD in this study may be the basis of inadequate TAC manifesting as increased MDA and reduced TPP levels. The attendant imbalance in oxidant/antioxidant ratio may result in abnormality in neuronal transduction leading to the abnormal cognitive and speech functions characteristic of ASD.


Assuntos
Transtorno do Espectro Autista , África ao Sul do Saara , Antioxidantes , Criança , Humanos , Malondialdeído , Estresse Oxidativo
10.
Molecules ; 26(19)2021 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-34641301

RESUMO

Sage, Salvia officinalis L., is used worldwide as an aromatic herb for culinary purposes as well as a traditional medicinal agent for various ailments. Current investigations exhibited the effects of extended dryings of the herb on the yields, composition, oil quality, and hepatoprotective as well as anti-cancer biological activities of the hydrodistillation-obtained essential oils from the aerial parts of the plant. The essential oils' yields, compositions, and biological activities levels of the fresh and differently timed and room-temperature dried herbs differed significantly. The lowest yields of the essential oil were obtained from the fresh herbs (FH, 631 mg, 0.16%), while the highest yield was obtained from the two-week dried herbs (2WDH, 1102 mg, 0.28%). A notable decrease in monoterpenes, with increment in the sesquiterpene constituents, was observed for the FH-based essential oil as compared to all the other batches of the essential oils obtained from the different-timed dried herbs. Additionally, characteristic chemotypic constituents of sage, i.e., α-pinene, camphene, ß-pinene, myrcene, 1, 8-cineole, α-thujone, and camphor, were present in significantly higher proportions in all the dried herbs' essential oils as compared to the FH-based essential oil. The in vivo hepatoprotective activity demonstrated significant reductions in the levels of AST, ALT, and ALP, as well as a significant increase in the total protein (p < 0.05) contents level, as compared to the acetaminophen (AAP) administered experimental group of rats. A significant reduction (p < 0.05) in the ALT level was demonstrated by the 4WDH-based essential oil in comparison to the FH-based essential oil. The levels of creatinine, cholesterol, and triglycerides were reduced (p < 0.05) in the pre-treated rats by the essential oil batches, with non-significant differences found among them as a result of the herbs dryings based oils. A notable increase in the viability of the cells, and total antioxidant capacity (TAOxC) levels, together with the reduction in malondialdehyde (MDA) levels were observed by the essential oils obtained from all the batches as compared with the AAP-treated cell-lines, HepG-2, HeLa, and MCF-7, that indicated the in vitro hepatoprotective effects of the sage essential oils. However, significant improvements in the in vivo and in vitro hepatoprotective activities with the 4WDH-based oil, as compared to all other essential oil-batches and silymarin standard demonstrated the beneficial effects of the drying protocol for the herb for its medicinal purposes.


Assuntos
Acetaminofen/efeitos adversos , Antineoplásicos Fitogênicos/administração & dosagem , Fígado/efeitos dos fármacos , Óleos Voláteis/administração & dosagem , Salvia officinalis/química , Animais , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/farmacologia , Cromatografia Gasosa-Espectrometria de Massas , Células HeLa , Células Hep G2 , Humanos , Fígado/metabolismo , Células MCF-7 , Masculino , Malondialdeído/metabolismo , Óleos Voláteis/química , Óleos Voláteis/farmacologia , Óleos Vegetais/administração & dosagem , Óleos Vegetais/química , Óleos Vegetais/farmacologia , Ratos , Ratos Wistar
11.
Molecules ; 26(20)2021 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-34684707

RESUMO

Cerebral ischemia results in increased oxidative stress in the affected brain. Accumulating evidence suggests that quercetin possesses anti-oxidant and anti-inflammatory properties. The essential elements magnesium (Mg), zinc (Zn), selenium (Se), and transition metal iron (Fe), copper (Cu), and antioxidants superoxide dismutase (SOD) and catalase (CAT) are required for brain functions. This study investigates whether the neuroprotective effects of quercetin on the ipsilateral brain cortex involve altered levels of essential trace metals, the Cu/Zn ratio, and antioxidant activity. Rats were intraperitoneally administered quercetin (20 mg/kg) once daily for 10 days before ischemic surgery. Cerebral ischemia was induced by ligation of the right middle cerebral artery and the right common carotid artery for 1 h. The ipsilateral brain cortex was homogenized and the supernatant was collected for biochemical analysis. Results show that rats pretreated with quercetin before ischemia significantly increased Mg, Zn, Se, SOD, and CAT levels, while the malondialdehyde, Fe, Cu, and the Cu/Zn ratio clearly decreased as compared to the untreated ligation subject. Taken together, our findings suggest that the mechanisms underlying the neuroprotective effects of quercetin during cerebral ischemic injury involve the modulation of essential elements, transition metals, Cu/Zn ratio, and antioxidant activity.


Assuntos
Isquemia Encefálica/tratamento farmacológico , Quercetina/farmacologia , Animais , Isquemia Encefálica/metabolismo , Isquemia Encefálica/fisiopatologia , Catalase , Cobre/metabolismo , Glutationa Peroxidase/metabolismo , Ferro , Peroxidação de Lipídeos/efeitos dos fármacos , Magnésio , Masculino , Malondialdeído , Fármacos Neuroprotetores/farmacologia , Estresse Oxidativo/efeitos dos fármacos , Quercetina/metabolismo , Ratos , Ratos Sprague-Dawley , Selênio , Superóxido Dismutase , Zinco/metabolismo
12.
PLoS One ; 16(9): e0257418, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34543299

RESUMO

One of the major causes of early pregnancy loss is heat stress. In ruminants, interferon tau (IFNT) is the embryo signal to the mother. Once the interferon signaling pathway is activated, it drives gene expression for interferon-stimulated genes (ISGs) and alters neutrophils responses. The aim of the present study was to evaluate interferon (IFN) pathway, ISGs and gene expression in polymorphonuclear leukocytes (PMN) and oxidative stress in dairy cows under heat stress. Pregnant cows had their estrous cycle synchronized and randomly assigned to a comfort or heat stress group. Blood samples were collected at artificial insemination (AI) and on Days 10, 14 and 18 following AI. Pregnant cows were pregnancy checked by ultrasound on Day 30 and confirmed on Day 60 post-AI. Results are presented as mean ± SEM. The corpus luteum (CL) diameter was not different between groups of pregnant cows; concentration of progesterone of pregnant cows on Day 18 following AI was greater in comfort group compared to heat stressed group. Comfort pregnant cows had higher expression of all analyzed genes from interferon pathway, except for IFNAR1, on both Days 14 and 18. Conversely, heat stressed cows did not show altered expression of IFNT pathway genes and ISGs between Days 10, 14, and 18 after AI. The oxidative stress, determined as malondialdehyde (MDA) levels, was greater in heat stress group on Days 10, 14 and 18, independent of pregnancy status. Heat stress negatively influences expression of ISGs, IFN pathway gene expression in neutrophils, and oxidative stress. Our data suggest that lower conception rates in cows under heat stress are multifactorial, with the association of interferon pathway activation and the unbalanced oxidative stress being main contributing factors.


Assuntos
Resposta ao Choque Térmico/genética , Interferons/metabolismo , Neutrófilos/metabolismo , Estresse Oxidativo , Animais , Bovinos , Corpo Lúteo/diagnóstico por imagem , Corpo Lúteo/fisiologia , Citocinas/genética , Citocinas/metabolismo , Feminino , Inseminação Artificial/veterinária , Malondialdeído/sangue , Proteínas de Resistência a Myxovirus/genética , Proteínas de Resistência a Myxovirus/metabolismo , Neutrófilos/citologia , Gravidez , Progesterona/sangue , Temperatura , Ultrassonografia
13.
Ulus Travma Acil Cerrahi Derg ; 27(5): 491-496, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34476792

RESUMO

BACKGROUND: Our aim in this study was to compare the blood gas changes, the malondialdehyde (MDA) and endogenous antioxidant glutathione (GSH) levels in blood and lung tissues after ischemia/reperfusion, the histopathological damage in lung tissue in rats provided respiratory support with mechanical ventilation after translaryngeal intubation and tracheostomy. METHODS: Group 1 rats were provided mechanical ventilator support after translaryngeal intubation, Group 2 mechanical ventilator support after tracheostomy, and Group 3 was the control group where rats were only anesthetized. Three groups were compared for blood gas changes, MDA, GSH, and histopathological changes. RESULTS: Blood gas evaluation showed a more marked increase in pO2 values and decline in pCO2 values in Group 2 than Group 1 (p<0.05), and higher serum MDA levels in Group 1 than Group 2 (p<0.05). Tissue GSH levels in Groups 1 and 2 were higher than the control group, but this difference was not statistically significant (p>0.05). In terms of histopathological scoring, the damage score in Group 1 was higher than in Group 2 (p<0.05). CONCLUSION: This is the first study to show tracheostomy to be more advantageous than translaryngeal intubation in terms of blood gases, ischemia/reperfusion damage, and structural changes in the lung tissue.


Assuntos
Traumatismo por Reperfusão , Traqueostomia , Animais , Radicais Livres , Intubação Intratraqueal , Malondialdeído , Ratos , Respiração Artificial
14.
Int J Mol Sci ; 22(18)2021 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-34576235

RESUMO

This study examined ceruloplasmin levels in patients with HFrEF, depending on cardiopulmonary exercise testing (CPET) parameters; a correlation was found between ceruloplasmin (CER) and iron and hepatic status, inflammatory and redox biomarkers. A group of 552 patients was divided according to Weber's classification: there were 72 (13%) patients in class A (peak VO2 > 20 mL/kg/min), 116 (21%) patients in class B (peak VO2 16-20 mL/kg/min), 276 (50%) patients in class C (peak VO2 10-15.9 mL/kg/min) and 88 (16%) patients in class D (peak VO2 < 10 mL/kg/min). A higher concentration of CER was found in patients with peak VO2 < 16 mL/kg/min and VE/CO2 slope > 45 compared to patients with VE/CO2 slope < 45 (escectively CER 30.6 mg/dL and 27.5 mg/dL). A significantly positive correlation was found between ceruloplasmin and NYHA class, RV diameter, NT-proBNP, uric acid, total protein, fibrinogen and hepatic enzymes. CER was positively correlated with both total oxidant status (TOS), total antioxidant capacity (TAC) and malondialdehyde. A model constructed to predict CER concentration indicated that TOS, malondialdehyde and alkaline phosphatase were independent predictive variables (R2 0.14, p < 0.001). CER as a continuous variable was an independent predictor of pVO2 ≤ 12 mL/kg/min after adjustment for sex, age and BMI. These results provide the basis of a new classification to encourage the determination of CER as a useful biomarker in HFrEF.


Assuntos
Biomarcadores/sangue , Ceruloplasmina/biossíntese , Insuficiência Cardíaca/sangue , Inflamação , Oxidantes , Volume Sistólico , Adulto , Antioxidantes/farmacologia , Índice de Massa Corporal , Ceruloplasmina/metabolismo , Teste de Esforço , Feminino , Insuficiência Cardíaca/diagnóstico , Humanos , Fígado/enzimologia , Masculino , Malondialdeído , Pessoa de Meia-Idade , Oxirredução , Estresse Oxidativo , Consumo de Oxigênio , Análise de Regressão , Índice de Gravidade de Doença , Resultado do Tratamento
15.
Tohoku J Exp Med ; 255(1): 19-25, 2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-34497164

RESUMO

Airborne fine particulate matter with an aerodynamic diameter equal to or smaller than 2.5 µm (abbreviated as PM2.5) increases the risk of nasal lesions, but the underlying molecular mechanism has not been fully elucidated. In the atmosphere, the composition of PM2.5 collected varies in physical and chemical properties, which affects its damage to human health. Thus, we constructed artificial PM2.5 particles based on actual PM2.5 and investigated the in vivo effects of artificial PM2.5 exposure on the oxidative stress, inflammatory response, and nasal mucosa morphology of rats. The results showed that artificial PM2.5 is comparable in composition ratio, size, and morphology to actual PM2.5. This in vivo study indicated that artificial PM2.5 exposure reduces total superoxide dismutase and glutathione peroxidase activities, elevates malondialdehyde content in the nasal mucosa, and induces increased levels of pro-inflammatory mediators, including interleukin-1, interleukin-6 and tumor necrosis factor-α. Our data shows that artificial PM2.5 particles could be used for experimental study of PM2.5 toxicology, ensuring that the physical and chemical properties of experimental PM2.5 are relatively constant and allowing for repeatability of this research. Oxidative damage and inflammatory response may be the toxic mechanisms that cause nasal lesions after exposure to artificial PM2.5.


Assuntos
Inflamação/etiologia , Estresse Oxidativo/efeitos dos fármacos , Material Particulado/toxicidade , Poluentes Atmosféricos/química , Poluentes Atmosféricos/toxicidade , Animais , Feminino , Humanos , Inflamação/patologia , Mediadores da Inflamação/metabolismo , Exposição por Inalação , Malondialdeído/metabolismo , Modelos Animais , Mucosa Nasal/efeitos dos fármacos , Mucosa Nasal/metabolismo , Mucosa Nasal/patologia , Tamanho da Partícula , Material Particulado/química , Ratos , Ratos Sprague-Dawley
16.
West Afr J Med ; 38(8): 770-774, 2021 08 30.
Artigo em Inglês | MEDLINE | ID: mdl-34503698

RESUMO

BACKGROUND: Plasmodium falciparum infection, like any other clinical condition, is prone to generating free radicals. This can worsen patients' clinical presentations. Antioxidants do help in ameliorating these free radical effects. These antioxidants, especially vitamins, are sometimes given routinely to patients with Plasmodium falciparum infection of which it can be given according to the severity of this free radical injury. METHODOLOGY: A total number of qualified 245 patients that came for malaria parasite test between March and October, 2020 were recruited into the study. Patients on arrival at the laboratory had their samples collected for malaria parasite test and for the proposed biochemical parameters (MDA, GPx, SOD and TAS). Malaria parasite test was used to categorize the severity of Plasmodium falciparum infection. RESULTS: There were statistically significant differences (p<0.0001) in MDA, GPx, SOD and TAS among patients with negative MP, 1+ and >2+ on blood film for malaria parasite. Patients with >2+ MP had highest levels (2.21±0.40) while patients with negative blood film had lowest levels(0.8194±0.33) of MDA. Patients with >2+ had lowest levels of GPx (2406.41±1272.10), SOD (104.54±30.62) and TAS (1.18±.35) as against patients with negative MP that had highest levels (5229.85±.2957.95)( 206.41±36.70)( 2.40±.53), respectively. CONCLUSION: There was evidence of free radical generation as evidenced with raised plasma malondialdehyde in patients with Plasmodium falciparum infection. This was associated with severity of this infection. There was also corresponding decrease in measured antioxidants (GPx, SOD and TAS).


Assuntos
Antioxidantes , Malária Falciparum , Radicais Livres , Humanos , Malondialdeído , Plasmodium falciparum
17.
Life Sci ; 284: 119931, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34480934

RESUMO

AIMS: Retinal neovascularization is one of the visual disorders during the postmenopausal period or types two diabetes. Physical activities and also phytoestrogens with powerful antioxidant features have been widely considered to improve nervous system diseases. Therefore, this study investigated the effects of genistein, swimming exercise, and their co-treatment on retina angiogenesis, oxidative stress, and inflammation in diabetic-ovariectomized rats. MAIN METHODS: Wistar rats were randomly divided into six groups (n = 8 per group): sham, ovariectomized group (OVX), OVX + diabetes (OVX.D), OVX.D+ genistein (1 mg/kg, eight weeks; daily SC), OVX.D + exercise (eight weeks), and OVX.D+ genistein+exercise (eight weeks). At the end of 8 weeks, the retina was removed under anesthesia. The assessed effects of treatment were by measuring MiR-146a and miR-132 expression via RT-PCR, the protein levels of ERK, MMP-2, VEGF, and NF-κB via western blotting, inflammation, and oxidative stress markers levels via the Eliza. KEY FINDINGS: The results showed miR-132, miR-146b, and MMP-2, NF-κB, ERK, VEGF, TNF-α, IL-1ß proteins, and MDA factor in the OVX.D group were increased, but glutathione (GSH) was decreased in comparison with the sham and OVX groups. Both exercise and genistein treatment has reversed the disorder caused by diabetes. However, the combination of exercise and genistein was more effective than each treatment alone. SIGNIFICANCE: It can be concluded that the interaction of exercise and genistein on microRNAs and their target protein was affected in the inflammation, stress oxidative, and extracellular matrix metalloproteinase pathways, can leading to a decrease in impairment of retinal neovascularization of the ovariectomized diabetic rats.


Assuntos
Diabetes Mellitus Experimental/patologia , Genisteína/farmacologia , Inflamação/patologia , Ovariectomia , Estresse Oxidativo , Retina/patologia , Natação/fisiologia , Fator A de Crescimento do Endotélio Vascular/metabolismo , Animais , Biomarcadores/metabolismo , Diabetes Mellitus Experimental/metabolismo , MAP Quinases Reguladas por Sinal Extracelular/metabolismo , Feminino , Genisteína/administração & dosagem , Glucose/metabolismo , Glutationa/metabolismo , Homeostase/efeitos dos fármacos , Mediadores da Inflamação/metabolismo , Malondialdeído/metabolismo , Metaloproteinase 2 da Matriz/metabolismo , MicroRNAs/genética , MicroRNAs/metabolismo , NF-kappa B/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Fosforilação/efeitos dos fármacos , Ratos Wistar , Retina/efeitos dos fármacos
18.
Plant Physiol Biochem ; 167: 884-900, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34537578

RESUMO

It was aimed to examine the role of gibberellic acid (GA3) and NPK fertilizer in alleviating boron (B) toxicity in coriander (Coriandrum sativum L.) plants. Two weeks old C. sativum seedlings were subjected to different NPK fertilizers [low NPK (30 kg ha-1) and normal NPK (60 kg ha-1)], which were also supplied by GA3 (50 mg L-1), under varying levels of B i.e., 0, 200 and 400 mg kg-1 in the soil. Results revealed that B toxicity led to a substantial decreased in the plant growth and biomass, photosynthetic pigments, gas exchange characteristics, sugars and essential nutrients in the roots and shoots of C. sativum seedlings. However, B toxicity boosted the production of reactive oxygen species (ROS) by increasing the contents of malondialdehyde (MDA), which is the indication of oxidative stress in C. sativum seedlings and was also manifested by hydrogen peroxide (H2O2) contents and electrolyte leakage (EL) to the membrane bounded organelles. Although, activities of various antioxidative enzymes like superoxidase dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX), non-enzymatic antioxidants like phenolic, flavonoid, ascorbic acid and anthocyanin contents and organic acids from the roots such as oxalic acid, malic acid, formic acid, citric acid, acetic acid and fumaric acid contents were increased with the increasing levels of B in the soil. The application if NPK and GA3 mitigated B toxicity by stimulated plant growth and biomass, photosynthetic efficiency, nutritional status and antioxidant machinery of the plant by decreasing MDA contents, H2O2 initiation and EL (%) in the roots and leaves of C. sativum seedlings. In addition, the application of NPK and GA3 further decreased the organic acids exudation contents in the roots C. sativum seedlings. Research findings, therefore, suggested that NPK and GA3 application can ameliorate B toxicity in C. sativum seedlings and resulted in improved plant growth and composition under B stress as depicted by balanced contents of organic acids.


Assuntos
Coriandrum , Poluentes do Solo , Antioxidantes , Boro , Giberelinas , Peróxido de Hidrogênio , Malondialdeído , Estresse Oxidativo , Plântula/química , Solo , Poluentes do Solo/análise , Poluentes do Solo/toxicidade
19.
J Med Food ; 24(9): 997-1009, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34524027

RESUMO

Oxidative stress has been demonstrated to be associated with numerous aging-related diseases. Ethyl acetate fraction of Abelmoschus manihot (L.) Medic (EA) had been reported to possess strong radical-scavenging activity due to its rich content of flavonoids. This work aimed to determine the protective effects of EA against oxidative injuries in vivo and in vitro, as well as to explore the relevant mechanisms behind these effects. Pretreatment with EA significantly elevated cell viability of H2O2-induced HepG2 cells, reduced the reactive oxygen species level, decreased apoptotic cells, and inhibited activities of caspase 3/9. Meanwhile, EA pretreatment elevated the levels of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), while reduced malondialdehyde (MDA) generation and lactate dehydrogenase (LDH) release dose-dependently. In addition, EA modulated key marker genes expression of antioxidation and apoptosis-related signaling pathways at the messenger RNA (mRNA) and protein levels. In the animal studies, EA also significantly enhanced the antioxidant activity and reduced MDA generation in serum, liver, and brain of the D-galactose (D-gal)-induced mice. Furthermore, the histological analysis indicated that EA effectively alleviated liver and brain injury of mice induced by D-gal, dose-dependently. EA as a potential antioxidant agent promoted health and reduced the risk of aging-associated diseases.


Assuntos
Abelmoschus , Estresse Oxidativo/efeitos dos fármacos , Extratos Vegetais/farmacologia , Abelmoschus/química , Acetatos , Envelhecimento , Animais , Antioxidantes/farmacologia , Flores/química , Galactose , Células Hep G2 , Humanos , Peróxido de Hidrogênio , Malondialdeído , Camundongos , Superóxido Dismutase/metabolismo
20.
J Transl Med ; 19(1): 411, 2021 09 27.
Artigo em Inglês | MEDLINE | ID: mdl-34579742

RESUMO

BACKGROUND: Peritoneal dialysis (PD) is a form of dialysis to replace the function of kidney, that uses the peritoneum as a dialysis membrane to remove metabolites and water retained in the body. Vitamin D deficiency is prevalent in patients treated with PD. This research investigated the correlation between serum 25-hydroxyvitamin D [25(OH)D] concentration and anemia, microinflammation, and oxidative stress in PD patients. METHODS: 62 PD patients and 56 healthy volunteers were recruited in this research. Serum concentrations of 25(OH)D and basic parameters of anemia were detected. The correlation between serum 25(OH)D concentration with anemia, oxidative stress, and microinflammatory state were analyzed. RESULTS: In the PD group, the concentration of 25(OH)D was lower than the healthy control (HC) group (p < 0.001). Hemoglobin, red blood cell count (RBC), and total iron binding capacity (TIBC) in the PD group was significantly lower (all p < 0.001), while high-sensitivity C-reactive protein (hs-CRP), interleukin-6 (IL-6), and tumor necrosis factor α (TNF-α) concentrations were significantly higher, than the HC group (all p < 0.001). In the PD group, malondialdehyde (MDA) concentration was higher than in the HC group (p < 0.001), while superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were lower (both p < 0.001). Serum 25(OH)D exhibited positive correlation with hemoglobin (r = 0.4509, p = 0.0002), RBC (r = 0.3712, p = 0.0030), TIBC (r = 0.4700, p = 0.0001), SOD (r = 0.4992, p < 0.0001) and GSH-Px (r = 0.4312, p = 0.0005), and negative correlation with hs-CRP (r = - 0.4040, p = 0.0011), TNF-α (r = - 0.4721, p = 0.0001), IL-6 (r = - 0.5378, p < 0.0001) and MDA (r = - 0.3056, p = 0.0157). CONCLUSION: In conclusion, reduced serum 25(OH)D concentrations in PD patients contribute to anemia, oxidative stress and microinflammatory state.


Assuntos
Anemia , Diálise Peritoneal , Anemia/complicações , Humanos , Malondialdeído , Estresse Oxidativo , Vitamina D
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