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1.
Sci Rep ; 11(1): 16706, 2021 08 18.
Artigo em Inglês | MEDLINE | ID: mdl-34408176

RESUMO

Although a canine adenovirus (CAdV)-based oncolytic virus (OV) candidate targeting canine tumors has been reported, its oncolytic effect could be attenuated by CAdV vaccine-induced neutralizing antibodies in dog patients. To circumvent this issue, we focused on the bat adenovirus (BtAdV) strain, which was previously isolated from healthy microbats. We previously showed that this virus replicated efficiently in canine cell lines and did not serologically cross-react with CAdVs, suggesting that it may offer the possibility of an OV candidate for canine tumors. Here, we tested the growth properties and cytotoxicity of the BtAdV Mm32 strain in a panel of canine tumor cells and found that its characteristics were equivalent to those of CAdVs. To produce an Mm32 construct with enhanced tumor specificity, we established a novel reverse genetics system for BtAdV based on bacterial artificial chromosomes, and generated a recombinant virus, Mm32-E1Ap + cTERTp, by inserting a tumor-specific canine telomerase reverse transcriptase promoter into its E1A regulatory region. The growth and cytotoxicity of this recombinant were superior to those of wild-type Mm32 in canine tumor cells, unlike in normal canine cells. These data suggest that Mm32-E1Ap + cTERTp could be a promising OV for alternative canine cancer therapies.


Assuntos
Quirópteros/virologia , Doenças do Cão/terapia , Mastadenovirus , Neoplasias , Terapia Viral Oncolítica , Vírus Oncolíticos , Animais , Linhagem Celular Tumoral , Cromossomos Artificiais Bacterianos/genética , Cães , Células Madin Darby de Rim Canino , Mastadenovirus/genética , Mastadenovirus/metabolismo , Neoplasias/terapia , Neoplasias/veterinária , Vírus Oncolíticos/genética , Vírus Oncolíticos/metabolismo
2.
J Med Virol ; 93(7): 4392-4398, 2021 07.
Artigo em Inglês | MEDLINE | ID: mdl-33829531

RESUMO

With the arrival of coronavirus disease 2019 (COVID-19) in Brazil in February 2020, several preventive measures were taken by the population aiming to avoid severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection including the use of masks, social distancing, and frequent hand washing then, these measures may have contributed to preventing infection also by other respiratory viruses. Our goal was to determine the frequencies of Influenza A and B viruses (FLUAV/FLUBV), human mastadenovirus C (HAdV-C), Enterovirus 68 (EV-68), and rhinovirus (RV) besides SARS-CoV-2 among hospitalized patients suspect of COVID-19 with cases of acute respiratory disease syndrome (ARDS) in the period of March to December 2020 and to detect possible coinfections among them. Nucleic acid detection was performed using reverse-transcription quantitative polymerase chain reaction (RT-qPCR) in respiratory samples using naso-oropharyngeal swabs and bronchoalveolar lavage. A total of 418 samples of the 987 analyzed (42.3%) were positive for SARS-CoV-2, 16 (1.62%) samples were positive for FLUAV, no sample was positive for FLUBV or EV-68, 67 (6.78%) samples were positive for HAdV-C, 55 samples were positive for RV 1/2 (26.3%) and 37 for RV 2/2 (13.6%). Coinfections were also detected, including a triple coinfection with SARS-CoV-2, FLUAV, and HAdV-C. In the present work, a very low frequency of FLUV was reported among hospitalized patients with ARDS compared to the past years, probably due to preventive measures taken to avoid COVID-19 and the high influenza vaccination coverage in the region in which this study was performed.


Assuntos
Infecções por Adenoviridae/epidemiologia , COVID-19/epidemiologia , Resfriado Comum/epidemiologia , Infecções por Enterovirus/epidemiologia , Influenza Humana/epidemiologia , Distanciamento Físico , Infecções por Adenoviridae/prevenção & controle , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Brasil/epidemiologia , COVID-19/prevenção & controle , Criança , Pré-Escolar , Coinfecção/epidemiologia , Coinfecção/virologia , Resfriado Comum/prevenção & controle , Enterovirus Humano D/genética , Enterovirus Humano D/isolamento & purificação , Infecções por Enterovirus/prevenção & controle , Feminino , Humanos , Lactente , Vírus da Influenza A/genética , Vírus da Influenza A/isolamento & purificação , Vírus da Influenza B/genética , Vírus da Influenza B/isolamento & purificação , Influenza Humana/prevenção & controle , Masculino , Máscaras , Mastadenovirus/genética , Mastadenovirus/isolamento & purificação , Pessoa de Meia-Idade , Técnicas de Amplificação de Ácido Nucleico/métodos , Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos , Rhinovirus/genética , Rhinovirus/isolamento & purificação , SARS-CoV-2/genética , SARS-CoV-2/isolamento & purificação , Adulto Jovem
3.
Sci Rep ; 11(1): 6331, 2021 03 18.
Artigo em Inglês | MEDLINE | ID: mdl-33737691

RESUMO

Few publications, often limited to one specific pathogen, have studied bonobos (Pan paniscus), our closest living relatives, as possible reservoirs of certain human infectious agents. Here, 91 stool samples from semicaptive bonobos and bonobos reintroduced in the wild, in the Democratic Republic of the Congo, were screened for different infectious agents: viruses, bacteria and parasites. We showed the presence of potentially zoonotic viral, bacterial or parasitic agents in stool samples, sometimes coinfecting the same individuals. A high prevalence of Human mastadenoviruses (HAdV-C, HAdV-B, HAdV-E) was observed. Encephalomyocarditis viruses were identified in semicaptive bonobos, although identified genotypes were different from those identified in the previous fatal myocarditis epidemic at the same site in 2009. Non-pallidum Treponema spp. including symbiotic T. succinifaciens, T. berlinense and several potential new species with unknown pathogenicity were identified. We detected DNA of non-tuberculosis Mycobacterium spp., Acinetobacter spp., Salmonella spp. as well as pathogenic Leptospira interrogans. Zoonotic parasites such as Taenia solium and Strongyloides stercoralis were predominantly present in wild bonobos, while Giardia lamblia was found only in bonobos in contact with humans, suggesting a possible exchange. One third of bonobos carried Oesophagostomum spp., particularly zoonotic O. stephanostomum and O. bifurcum-like species, as well as other uncharacterized Nematoda. Trypanosoma theileri has been identified in semicaptive bonobos. Pathogens typically known to be transmitted sexually were not identified. We present here the results of a reasonably-sized screening study detecting DNA/RNA sequence evidence of potentially pathogenic viruses and microorganisms in bonobo based on a noninvasive sampling method (feces) and focused PCR diagnostics.


Assuntos
Espécies em Perigo de Extinção , Interações Hospedeiro-Patógeno/genética , Mastadenovirus/isolamento & purificação , Pan paniscus/virologia , Animais , República Democrática do Congo/epidemiologia , Vírus da Encefalomiocardite/isolamento & purificação , Vírus da Encefalomiocardite/patogenicidade , Fezes/microbiologia , Fezes/parasitologia , Fezes/virologia , Humanos , Mastadenovirus/patogenicidade , Pan paniscus/microbiologia , Pan paniscus/parasitologia , Pan troglodytes/microbiologia , Pan troglodytes/parasitologia , Pan troglodytes/virologia , Parasitos/isolamento & purificação , Parasitos/patogenicidade
4.
Res Vet Sci ; 135: 450-455, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33203584

RESUMO

BRD is associated with infectious agents, but management and transport-stress are trigger factors. Metaphylactic administration of antimicrobial reduces colonization of respiratory tract by pathogens, but the development of antibiotic-resistance raises public health concerns leading to propose new control strategies. The study analyzed nasopharyngeal swabs of 231 imported cattle, 10% of 49 trucks, transported from France to southern Italy and, through Real-time PCR identified the prevalence of the involved pathogens speculating on strategies to reduce the impact of BRD. The samples were tested by Real-time PCR, for the detection of bovine coronavirus (BCoV), bovine respiratory syncytial virus (BRSV), bovine parainfluenza virus (BPiV), bovine adenovirus (BAdV), Mannheimia haemolytica, Pasteurella multocida, Histophilus somni, and Mycoplasma bovis. Yates-corrected chi squared, or Fisher's exact test were used to compare both animal-health status and positivity/negativity to pathogens, and the relationship between presence/absence of clinical signs and Real-time PCR-positivity. H. somni and BCoV were the most frequently identified pathogens. In BRD-diagnosed cattle, BAdV was detected in 13.8% (19/138), BRSV in 14.5% (20/138) and BPiV in 4.3% (6/138). Healthy cattle were mostly positive for H. somni (89.2%, 83/93). A statistically significant association was observed between clinical signs and positivity to M. haemolytica (p value = 0.016). Although mass-medication and vaccination are used for BRD control, it still remains a primary health problem. Our results highlight that the nasopharyngeal microbiota could be affected by transport and that strategies to enhance calf immunity for reducing BRD-risk development would be more effective if applied at farm of origin prior to loading.


Assuntos
Doenças dos Bovinos/epidemiologia , Coronavirus Bovino/isolamento & purificação , Microbiota , Pasteurellaceae/isolamento & purificação , Doenças Respiratórias/veterinária , Animais , Bovinos , Doenças dos Bovinos/microbiologia , Doenças dos Bovinos/prevenção & controle , Coronavirus Bovino/genética , Estudos Epidemiológicos , França/epidemiologia , Imunidade , Itália/epidemiologia , Masculino , Mastadenovirus/genética , Mastadenovirus/isolamento & purificação , Nasofaringe/microbiologia , Pasteurellaceae/genética , Vírus Sincicial Respiratório Bovino/genética , Vírus Sincicial Respiratório Bovino/isolamento & purificação , Sistema Respiratório/microbiologia , Doenças Respiratórias/epidemiologia , Doenças Respiratórias/microbiologia , Doenças Respiratórias/prevenção & controle , Respirovirus/genética , Respirovirus/isolamento & purificação , Transportes
5.
Viruses ; 12(12)2020 12 12.
Artigo em Inglês | MEDLINE | ID: mdl-33322850

RESUMO

A number of characteristics including lack of virulence and the ability to grow to high titers, have made bovine adenovirus-3 (BAdV-3) a vector of choice for further development as a vaccine-delivery vehicle for cattle. Despite the importance of blood leukocytes, including dendritic cells (DC), in the induction of protective immune responses, little is known about the interaction between BAdV-3 and bovine blood leukocytes. Here, we demonstrate that compared to other leukocytes, bovine blood monocytes and neutrophils are significantly transduced by BAdV404a (BAdV-3, expressing enhanced yellow green fluorescent protein [EYFP]) at a MOI of 1-5 without a significant difference in the mean fluorescence of EYFP expression. Moreover, though expression of some BAdV-3-specific proteins was observed, no progeny virions were detected in the transduced monocytes or neutrophils. Interestingly, addition of the "RGD" motif at the C-terminus of BAdV-3 minor capsid protein pIX (BAV888) enhanced the ability of the virus to enter the monocytes without altering the tropism of BAdV-3. The increased uptake of BAV888 by monocytes was associated with a significant increase in viral genome copies and the abundance of EYFP and BAdV-3 19K transcripts compared to BAdV404a-transduced monocytes. Our results suggest that BAdV-3 efficiently transduces monocytes and neutrophils in the absence of viral replication. Moreover, RGD-modified capsid significantly increases vector uptake without affecting the initial interaction with monocytes.


Assuntos
Infecções por Adenoviridae/veterinária , Doenças dos Bovinos/virologia , Leucócitos/virologia , Mastadenovirus/fisiologia , Tropismo Viral , Animais , Bovinos , Doenças dos Bovinos/imunologia , Doenças dos Bovinos/metabolismo , Linhagem Celular , Expressão Gênica , Regulação Viral da Expressão Gênica , Leucócitos/imunologia , Leucócitos/metabolismo , Transdução Genética , Proteínas Virais/genética , Proteínas Virais/metabolismo , Replicação Viral
6.
Acta Vet Hung ; 68(3): 323-327, 2020 09 30.
Artigo em Inglês | MEDLINE | ID: mdl-33128521

RESUMO

The complete genomic sequence along with phylogenetic analyses of an adenovirus (AdV), isolated from a dead captive pygmy marmoset (Callithrix pygmaea) from a Hungarian zoo is reported. Earlier, based on the phylogenetic analysis of the sequence of a PCR-amplified fragment from the DNA polymerase gene, the pygmy marmoset AdV (PMAdV) has been reported to cluster closest to certain chiropteran AdVs. In the following years similar AdVs were discovered in additional mammalian hosts, including a skunk (Mephitis mephitis), African pygmy hedgehogs (Atelerix albiventris), North American porcupines (Erethizon dorsatum) and grey fox (Urocyon cinereoargenteus). After the full genome analysis of the skunk adenovirus (SkAdV-1), a novel species Skunk mastadenovirus A (SkAdV-A) has been established. The AdVs, originating from the African pygmy hedgehogs, have been found to belong to virus species SkAdV-A. Partial gene sequences from the porcupine AdVs have also implied their very close genetic relatedness to SkAdV-A. The complete genomic sequence of PMAdV, examined in this study, was found to share 99.83% nucleotide identity with SkAdV-1, thus unequivocally represents a genomic variant of SkAdV-1. The observation that viruses classifiable as SkAdV-A are able to infect and cause diseases in several, distantly related mammals seems to deserve further studies to elucidate the infection biology of this intriguing AdV.


Assuntos
Callithrix/virologia , Genoma Viral , Mastadenovirus/genética , Mephitidae/virologia , Animais , Mastadenovirus/classificação , Sequenciamento Completo do Genoma/veterinária
7.
Viruses ; 12(6)2020 06 18.
Artigo em Inglês | MEDLINE | ID: mdl-32570742

RESUMO

Non-human primates (NHPs) are known hosts for adenoviruses (AdVs), so there is the possibility of the zoonotic or cross-species transmission of AdVs. As with humans, AdV infections in animals can cause diseases that range from asymptomatic to fatal. The aim of this study was to investigate the occurrence and diversity of AdVs in: (i) fecal samples of apes and monkeys from different African countries (Republic of Congo, Senegal, Djibouti and Algeria), (ii) stool of humans living near gorillas in the Republic of Congo, in order to explore the potential zoonotic risks. Samples were screened by real-time and standard PCRs, followed by the sequencing of the partial DNA polymerase gene in order to identify the AdV species. The prevalence was 3.3 folds higher in NHPs than in humans. More than 1/3 (35.8%) of the NHPs and 1/10 (10.5%) of the humans excreted AdVs in their feces. The positive rate was high in great apes (46%), with a maximum of 54.2% in chimpanzees (Pan troglodytes) and 35.9% in gorillas (Gorilla gorilla), followed by monkeys (25.6%), with 27.5% in Barbary macaques (Macaca sylvanus) and 23.1% in baboons (seven Papio papio and six Papio hamadryas). No green monkeys (Chlorocebus sabaeus) were found to be positive for AdVs. The AdVs detected in NHPs were members of Human mastadenovirus E (HAdV-E), HAdV-C or HAdV-B, and those in the humans belonged to HAdV-C or HAdV-D. HAdV-C members were detected in both gorillas and humans, with evidence of zoonotic transmission since phylogenetic analysis revealed that gorilla AdVs belonging to HAdV-C were genetically identical to strains detected in humans who had been living around gorillas, and, inversely, a HAdV-C member HAdV type was detected in gorillas. This confirms the gorilla-to-human transmission of adenovirus. which has been reported previously. In addition, HAdV-E members, the most often detected here, are widely distributed among NHP species regardless of their origin, i.e., HAdV-E members seem to lack host specificity. Virus isolation was successful from a human sample and the strain of the Mbo024 genome, of 35 kb, that was identified as belonging to HAdV-D, exhibited close identity to HAdV-D members for all genes. This study provides information on the AdVs that infect African NHPs and the human populations living nearby, with an evident zoonotic transmission. It is likely that AdVs crossed the species barrier between different NHP species (especially HAdV-E members), between NHPs and humans (especially HAdV-C), but also between humans, NHPs and other animal species.


Assuntos
Infecções por Adenoviridae/epidemiologia , Infecções por Adenoviridae/veterinária , Mastadenovirus/classificação , Mastadenovirus/isolamento & purificação , Infecções por Adenoviridae/transmissão , Argélia/epidemiologia , Animais , Chlorocebus aethiops/virologia , Congo/epidemiologia , DNA Viral/genética , DNA Polimerase Dirigida por DNA/genética , Djibuti/epidemiologia , Fezes/virologia , Gorilla gorilla/virologia , Humanos , Macaca/virologia , Mastadenovirus/genética , Pan troglodytes/virologia , Papio hamadryas/virologia , Papio papio/virologia , Senegal/epidemiologia , Zoonoses Virais/epidemiologia , Zoonoses Virais/transmissão
8.
Virology ; 547: 12-19, 2020 08.
Artigo em Inglês | MEDLINE | ID: mdl-32560900

RESUMO

CD8 T cells contribute to effective clearance of mouse adenovirus type 1 (MAV-1) and to virus-induced pulmonary inflammation. We characterized effects of a CD8 T cell effector, TNF, on MAV-1 pathogenesis. TNF inhibited MAV-1 replication in vitro. TNF deficiency or immunoneutralization had no effect on lung viral loads or viral gene expression in mice infected intranasally with MAV-1. Absence of TNF delayed virus-induced weight loss and reduced histological evidence of pulmonary inflammation, although concentrations of proinflammatory cytokines and chemokines in bronchoalveolar lavage fluid (BALF) were not significantly affected. BALF concentrations of IL-10 were greater in TNF-deficient mice compared to controls. Our data indicate that TNF is not essential for control of viral replication in vivo, but virus-induced TNF contributes to some aspects of immunopathology and disease. Redundant CD8 T cell effectors and other aspects of immune function are sufficient for antiviral and pro-inflammatory responses to acute MAV-1 respiratory infection.


Assuntos
Infecções por Adenoviridae/virologia , Mastadenovirus/fisiologia , Pneumonia/imunologia , Fator de Necrose Tumoral alfa/imunologia , Replicação Viral , Infecções por Adenoviridae/genética , Infecções por Adenoviridae/imunologia , Animais , Linfócitos T CD8-Positivos/imunologia , Humanos , Interleucina-10/genética , Interleucina-10/imunologia , Pulmão/imunologia , Pulmão/virologia , Masculino , Mastadenovirus/genética , Camundongos , Camundongos Endogâmicos C57BL , Pneumonia/genética , Pneumonia/virologia , Fator de Necrose Tumoral alfa/genética
9.
Infect Genet Evol ; 83: 104348, 2020 09.
Artigo em Inglês | MEDLINE | ID: mdl-32380313

RESUMO

Bovine adenovirus type 3 (BAdV-3) is an important pathogen causing bovine respiratory disease complex (BRDC). From Jun 2016 to Jun 2018, 108 nose swab samples were collected from cattle with BRDC from 11 farms in five cities, and 78.7% (85/108) samples were detected as BAdV-3 positive by Real-time PCR. Interestingly, the sequences of 7/10 fiber (852 bp) and hexon (785 bp) fragments cloned from 10 positive samples from eight farms were clustered into a single branch of the evolutionary tree. A BAdV-3 strain (BO/YB24/17/CH) was successfully isolated. The isolate caused pathological changes of lung, trachea and spleen in BALB/c mice. Notably, 79 amino acid deletions in the shaft domain and 74 unique amino acid mutations were found in the fiber gene of the isolate compared with the available complete sequences for fiber genes in the GenBank database. These characteristics indicated that the isolate may represent a novel fiber genotype of BAdV-3. A pair of specific primers covering the deletion region in the fiber gene was designed to screen the prevalence of BAdV-3 encoding the novel fiber gene. The results showed that 7 of the 10 strains possessed the novel fiber gene, and these novel fiber strains were detected from six farms in which calves were just imported from five provinces, indicating that this BAdV-3 with the natural deletion fiber gene has a wide geographical distribution in China. In conclusion, our results reveal that BAdV-3 is widespread in China and a pathogenic BAdV-3 strain with a novel fiber gene has been detected at high frequency, which is beneficial to understand the prevalence and genetic evolution of BAdV-3.


Assuntos
Infecções por Adenoviridae/virologia , Doenças dos Bovinos/virologia , Mastadenovirus/genética , Proteínas Virais/genética , Infecções por Adenoviridae/epidemiologia , Infecções por Adenoviridae/veterinária , Animais , Bovinos , Doenças dos Bovinos/epidemiologia , China/epidemiologia , Deleção de Genes , Mastadenovirus/isolamento & purificação , Mastadenovirus/patogenicidade , Camundongos Endogâmicos BALB C , Epidemiologia Molecular , Filogenia , Prevalência , Reação em Cadeia da Polimerase em Tempo Real
10.
Jpn J Infect Dis ; 73(5): 349-353, 2020 Sep 24.
Artigo em Inglês | MEDLINE | ID: mdl-32350225

RESUMO

Seven human mastadenovirus (HAdV) species (A-G) are known with more than 100 reported types. HAdV is highly resistant to common hand sanitizers. Epidemic keratoconjunctivitis and pharyngoconjunctival fever are caused by HAdV, which can be explosively transmitted in a confined space, resulting in outbreaks, such as nosocomial infections. Given the absence of an antiviral agent against the HAdV infection, it is important to prevent the spread of the infection by using disinfectants. Ozone has already been well-known for its bactericidal and virucidal effects. ALTANT is an ozonated alcohol preparation developed by E-TECH Co., Ltd. (Kobe, Hyogo, Japan). In this study, we mixed ALTANT with different HAdV types at a ratio of 9:1 and determined HAdV viability after instantaneous reactions for varying periods (flash to 5 minutes) using the TCID50 assay. The assay results demonstrated that the HAdV viability decreased by 1/10 to 1/100 within 1 minute after the reaction; additionally, slight differences in the reactivity were observed among the HAdV types. HAdV viability decreased by a factor of > 4log10, and the virus was eliminated within 3 minutes. This study demonstrated the potent HAdV disinfection effect of ALTANT.


Assuntos
Infecções por Adenovirus Humanos/prevenção & controle , Desinfetantes/farmacologia , Etanol/farmacologia , Mastadenovirus/efeitos dos fármacos , Ozônio/farmacologia , Infecções por Adenovirus Humanos/virologia , Adenovírus Humanos , Anti-Infecciosos Locais/química , Anti-Infecciosos Locais/farmacologia , Proliferação de Células/efeitos dos fármacos , Infecção Hospitalar/prevenção & controle , Infecção Hospitalar/virologia , Surtos de Doenças , Desinfetantes/química , Etanol/química , Humanos , Japão , Ceratoconjuntivite/prevenção & controle , Ceratoconjuntivite/virologia , Mastadenovirus/patogenicidade , Testes de Sensibilidade Microbiana , Ozônio/química
11.
Virology ; 546: 25-37, 2020 07.
Artigo em Inglês | MEDLINE | ID: mdl-32452415

RESUMO

Bovine adenovirus-3 (BAdV-3) is a non enveloped, icosahedral DNA virus containing a genome of 34446 bps. The intermediate region of BAdV-3 encodes pIX and IVa2 proteins. Here, we report the characterization of BAdV-3 IVa2. Anti-IVa2 serum detected a 50 kDa protein at 24-48 h post infection in BAdV-3 infected cells. The IVa2 localizes to nucleus and nucleolus of BAdV-3 infected cells. Analysis of mutant IVa2 demonstrated that amino acids 1-25 and 373-448 are required for nuclear and nucleolar localization of IVa2, respectively. The nuclear import of IVa2 utilize importin α -1 of importin nuclear import pathway. Although deletion/substitution of amino acids 4-18 is sufficient to abrogate the nuclear localization of IVa2, amino acids 1-25 are required for nuclear localization of a cytoplasmic protein. Furthermore, we demonstrate that amino acids 1-25 and 120-140 of IVa2 interact with importin α-1 and pV proteins, respectively in BAdV-3 infected cells.


Assuntos
Infecções por Adenoviridae/veterinária , Doenças dos Bovinos/virologia , Nucléolo Celular/virologia , Mastadenovirus/metabolismo , Proteínas Virais/química , Proteínas Virais/metabolismo , Transporte Ativo do Núcleo Celular , Infecções por Adenoviridae/genética , Infecções por Adenoviridae/metabolismo , Infecções por Adenoviridae/virologia , Motivos de Aminoácidos , Animais , Bovinos , Doenças dos Bovinos/genética , Doenças dos Bovinos/metabolismo , Nucléolo Celular/genética , Nucléolo Celular/metabolismo , Núcleo Celular/genética , Núcleo Celular/metabolismo , Núcleo Celular/virologia , Genoma Viral , Carioferinas/genética , Carioferinas/metabolismo , Mastadenovirus/química , Mastadenovirus/genética , Ligação Proteica , Transporte Proteico , Proteínas Virais/genética
12.
Virol Sin ; 35(5): 528-537, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32236817

RESUMO

Porcine adenoviruses (PAdVs) are classified into three species, PAdV-A, PAdV-B, and PAdV-C. The genomes of PAdV-A and PAdV-C have been well characterized. However, the genome of PAdV-B has never been completely sequenced, and the epidemiology of PAdV-B remains unclear. In our study, we have identified a novel strain of PAdV-B, named PAdV-B-HNU1, in porcine samples collected in China by viral metagenomic assay and general PCR. The genome of PAdV-B-HNU1 is 31,743 bp in length and highly similar to that of California sea lion adenovirus 1 (C. sea lion AdV-1), which contains typical mastadenoviral structures and some unique regions at the carboxy-terminal end. Especially, PAdV-B-HNU1 harbors a dUTPase coding region not clustering with other mastadenoviruses except for C. sea lion AdV-1 and a fiber coding region homologous with galectin 4 and 9 of animals. However, the variance of GC contents between PAdV-B-HNU1 (55%) and C. sea lion AdV-1 (36%) indicates their differential evolutionary paths. Further epidemiologic study revealed a high positive rate (51.7%) of PAdV-B-HNU1 in porcine lymph samples, but low positive rates of 10.2% and 16.1% in oral swabs and rectal swabs, respectively. In conclusion, this study characterized a novel representative genome of a lymphotropic PAdV-B with unique evolutionary origin, which contributes to the taxonomical and pathogenic studies of PAdVs.


Assuntos
Adenovirus Suínos , Mastadenovirus , Adenovirus Suínos/genética , Animais , Sequência de Bases , China , Genoma Viral , Mastadenovirus/genética , Fases de Leitura Aberta , Suínos
13.
Virus Res ; 285: 197965, 2020 08.
Artigo em Inglês | MEDLINE | ID: mdl-32311385

RESUMO

Next generation sequencing was used to determine the whole genome sequence for two different strains of guinea pig adenovirus (GPAdV) detected in association with outbreaks of pneumonia in Australia in 1996, and in Germany in 1997 using total DNA extracted from infected archival frozen lung tissue as a template. The length of the determined genomic sequences was 37,031 bp and 37,070 bp, respectively. The nucleotide composition showed a relatively high content of guanine + cytosine (G + C) of 62 %. The 99.6 % nucleotide identity between the two sequenced viruses suggests that they may represent variants of the same genotype. The GPAdV genome exhibits the genomic features of a typical mastadenovirus with at least 32 open reading frames identified. Five novel open reading frames were found at the right end of the genomic sequence. One of them maps to the predicted E3 region and encodes a putative CR1 protein, two map to the E4 region, and two map to the l strand of L1 and L3, respectively. Our phylogenetic analysis of whole genome sequences showed that among the mammalian AdV species described to date, GPAdV is most closely related to MAdV-2 The characterization of this mastadenovirus species offers an opportunity to develop a new small animal model to study mammalian adenovirus pathogenesis.


Assuntos
Infecções por Adenoviridae , Pulmão/virologia , Mastadenovirus , Infecções por Adenoviridae/veterinária , Infecções por Adenoviridae/virologia , Animais , DNA Viral , Genoma Viral , Cobaias , Mastadenovirus/classificação , Mastadenovirus/isolamento & purificação , Filogenia , Sequenciamento Completo do Genoma
14.
Virus Res ; 277: 197846, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-31870796

RESUMO

The presence of a novel adenovirus (AdV) was detected by PCR and sequencing, in the internal organs of a captive polar bear that had died in the Budapest zoo. The virus content of the samples proved to be high enough to allow for conventional Sanger sequencing on PCR-amplified genomic fragments. With this approach, the sequence of the entire genome of the putative polar bear adenovirus 1 (PBAdV-1) was obtained. Although the genome was found to be short, consisting of 27,952 base pairs merely, with a relatively balanced G + C content of 46.3 %, its organisation corresponded largely to that of a typical mastadenovirus. Every genus-common gene could be identified except that of protein IX. The short E3 region of the PBAdV-1 consisted of two novel, supposedly type-specific ORFs only, whereas no homologue of any of the E3 genes, usually conserved in mastadenoviruses, such as for example that of the 12.5 K protein, were present. In the E4 region, only the highly conserved gene of the 34 K protein was found besides two novel ORFs showing no homology to any known E4 ORFs. In silico sequence analysis revealed putative splicing donor and acceptor sites in the genes of the E1A, IVa2, DNA-dependent DNA polymerase, pTP, 33 K proteins, and also of U exon protein, all being characteristic for mastadenoviruses. Phylogenetic calculations, based on various proteins, further supported that the newly-detected PBAdV is the representative of a new species within the genus Mastadenovirus, and may represent the evolutionary lineage of adenoviruses that coevolved with carnivorans.


Assuntos
Infecções por Adenoviridae/veterinária , Genoma Viral , Mastadenovirus/classificação , Filogenia , Ursidae/virologia , Infecções por Adenoviridae/virologia , Animais , Animais de Zoológico/virologia , DNA Viral/genética , Feminino , Mastadenovirus/isolamento & purificação , Análise de Sequência de DNA , Proteínas Virais/genética , Sequenciamento Completo do Genoma
15.
FEBS Lett ; 593(24): 3649-3659, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31777948

RESUMO

Small laboratory animals are powerful models for investigating in vivo viral pathogenesis of a number of viruses. For adenoviruses (AdVs), however, species-specificity poses limitations to studying human adenoviruses (HAdVs) in mice and other small laboratory animals. Thus, this review covers work on naturally occurring mouse AdVs, primarily mouse adenovirus type 1 (MAdV-1), a member of the species Murine mastadenovirus A. Molecular genetics, virus life cycle, cell and tissue tropism, interactions with the host immune response, persistence, and host genetics of susceptibility are described. A brief discussion of MAdV-2 (member of species Murine mastadenovirus B) and MAdV-3 (member of species Murine mastadenovirus C) is included. We report the use of MAdVs in the development of vectors and vaccines.


Assuntos
Infecções por Adenoviridae/veterinária , Mastadenovirus/patogenicidade , Animais , Regulação Viral da Expressão Gênica , Interações Hospedeiro-Patógeno , Mastadenovirus/genética , Mastadenovirus/fisiologia , Camundongos , Especificidade da Espécie , Proteínas Virais/genética , Tropismo Viral
16.
Artigo em Inglês | MEDLINE | ID: mdl-31778390

RESUMO

Public parks are leisure environments widely used by both, adults and children, often accompained by their pets. Soil contamination of these environments by enteric viruses and intestinal parasites occurs through these animals feces. The aim of this work was to detect Carnivore protoparvovirus 1 (CPV-1) and different species of Mastadenovirus in soils samples from a park located in a medium-sized city in Brazil and evaluate the presence of helminth eggs and larvae in 18 points of a public park soil samples, as well as feces found on this site during six months. Parasitological analyzes were conducted through flotation and sedimentation techniques, and polymerase chain reaction (PCR) was used for viral detection. Of the 216 soil and 16 feces samples, 49% (106/216) and 12% (2/16) were positivefor nematodes larvae, respectively, through sedimentation techniques. Toxocara spp eggs were found in one soil sample and one feces sample, Trichuris spp eggs were found in only one feces sample and Hookworms eggs were found in four soil samples. After reconstruction work in the streets near the park, 30% (64/216) of the samples were positive for Human Mastadenovirus C (HAdV-C), 1.4% (3/216) for HAdV-E and 0.4% (1/216) for Canine Mastadenovirus A (CAdV-A). The parasitic forms found in this study have demonstrated that the contamination of the park's soil pose a threat to human and animal health. This was the first study to report the presence of HAdVs and CAdVs in soil samples.


Assuntos
Ancylostomatoidea/isolamento & purificação , Mastadenovirus/isolamento & purificação , Microbiologia do Solo , Solo/parasitologia , Toxocara/isolamento & purificação , Ancylostomatoidea/classificação , Ancylostomatoidea/genética , Animais , Cães , Fezes/parasitologia , Humanos , Mastadenovirus/classificação , Mastadenovirus/genética , Parques Recreativos , Reação em Cadeia da Polimerase em Tempo Real , Toxocara/classificação , Toxocara/genética
17.
Virus Genes ; 55(6): 769-778, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31385187

RESUMO

Human mastadenoviruses (HAdVs) are non-enveloped, double-stranded DNA viruses that are comprised of more than 85 types classified within seven species (A-G) based on genomics. All HAdV prototypes and many newly defined type genomes have been completely sequenced and are available. Computational analyses of the prototypes and newly emergent HAdV strains provide insights into the evolutionary history and molecular adaptation of HAdV. Most types of HAdV-B are important pathogens causing severe respiratory infections or urinary tract infections and are well characterized. However, HAdV-16 of the B1 subspecies has rarely been reported and its genome is poorly characterized. In this study, bioinformatics analysis, based on genome sequences obtained in GenBank, suggested that HAdV-16, a prototype HAdV-B species, evolved from multiple intertypic recombination events. HAdV-16 genome contains the hexon loop 1 to loop 2 region from HAdV-E4, the partial hexon conserved region 4 (C4) from the subspecies HAdV-B2, genome region 30,897-33,384 containing the fiber gene from SAdV-35, and other genomic parts from the subspecies HAdV-B1. Moreover, analysis of sequence similarity with HAdV-E4 LI, LII, and SAdV-36 strains demonstrated the recombination events happened rather early. Further, amino acid sequence alignment indicated that the amino acid variations occurred in hypervariable regions (HVRs). Especially, the major difference in HVR7, which contains the critical neutralization epitope of HAdV-E4, between HAdV-16 and HAdV-E4 might explain the low level of cross-neutralization between these strains. Our findings promote better understanding on HAdV evolution, predicting newly emergent HAdV strains, and developing novel HAdV vectors.


Assuntos
Adenovírus Humanos/genética , Evolução Molecular , Mastadenovirus/genética , Recombinação Genética/genética , Adenovírus Humanos/classificação , Adenovírus Humanos/patogenicidade , Sequência de Aminoácidos/genética , Proteínas do Capsídeo/genética , Biologia Computacional , Epitopos/genética , Genoma Viral/genética , Humanos , Mastadenovirus/classificação , Mastadenovirus/patogenicidade , Filogenia , Sequenciamento Completo do Genoma
18.
Braz. j. microbiol ; 50(3): 677-684, July 2019. ilus., tab
Artigo em Inglês | LILACS, Coleciona SUS, CONASS, SES-RS | ID: biblio-1121770

RESUMO

Human mastadenovirus (HAdV) genus is related to several diseases, among them upper and lower respiratory tract illness. HAdV species B, C, D, and E are mainly associated with respiratory infections. The goal of this work was to identify the HAdV species associated with respiratory infections in hospitalized patients from southern Brazil. Samples were collected from 1996 to 2004 and 2011 to 2017. During this period, 28,524 samples were collected, and 9983 were positive for respiratory viruses, being 435 for HAdV. From these 435 samples, 57 were selected for characterization of HAdV species. For screening the presence of HAdV, a partial sequence of the DNA polymerase gene (DNApol gene) was amplified by nested PCR. Partial nucleotide sequencing was performed in positive samples, and HAdV (DNApol gene) was detected in 53 samples: species B (28;49.1%), C (16;8.0%), D (2; 3.5%), E (5; 8.7%), and untyped (2; 3.5%). Specie D was found only in 2017 and specie E in 2011 and 2012. The age of the patients ranged from < 1 to 81 years old, and 62.3%were male. No relationship between gender orage and identified HAdV species were observed. In addition, in the period of 2013­2017, 18 samples from patients who died were analyzed: 11 were related to species B, 4 to C, and 2 to D and 1 remained untyped. Circulation of HAdV species D and Evaried over the years, but species B and C were present throughout the evaluated period. In addition, respiratory infections by HAdVaffect elderly and children mainly. (AU)


Assuntos
Humanos , Masculino , Feminino , Criança , Idoso , Idoso de 80 Anos ou mais , Sistema Respiratório , Infecções Respiratórias/virologia , Mastadenovirus/patogenicidade , Ácidos Nucleicos , Morbidade
19.
Arch Virol ; 164(8): 2205-2207, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31152248

RESUMO

Pathological examination of a suckling male lamb showed severe viral pneumonia with suspected bacterial superinfection. Adenovirus was detected by immunohistochemical examination of the affected lung samples. Detection of the suspected adenovirus by PCR and subsequent isolation of the virus were successful. Using next-generation sequencing, the full genome of this ovine adenovirus was sequenced and analysed. A genome sequence comparison showed that it was a novel mastadenovirus type (named "ovine adenovirus 8") that did not belong to any of the established adenovirus species. The genome is 36,206 bp long, containing 93-bp inverted terminal repeats and 29 predicted genes, including the two genus-specific genes (encoding proteins V and IX). Ovine adenovirus 8 shows the closest relationship to ovine adenovirus 6. These two viruses seem to merit the establishment of a novel ovine mastadenovirus species for them, for which we proposed the name "Ovine mastadenovirus C".


Assuntos
Adenoviridae/genética , Genoma Viral/genética , Mastadenovirus/genética , Infecções por Adenoviridae/virologia , Animais , DNA Viral/genética , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Filogenia , Ovinos
20.
J Virol ; 93(15)2019 08 01.
Artigo em Inglês | MEDLINE | ID: mdl-31092582

RESUMO

Acute respiratory infection with mouse adenovirus type 1 (MAV-1) induces activity of the immunoproteasome, an inducible form of the proteasome that shapes CD8 T cell responses by enhancing peptide presentation by major histocompatibility complex (MHC) class I. We used mice deficient in all three immunoproteasome subunits (triple-knockout [TKO] mice) to determine whether immunoproteasome activity is essential for control of MAV-1 replication or inflammatory responses to acute infection. Complete immunoproteasome deficiency in adult TKO mice had no effect on MAV-1 replication, virus-induced lung inflammation, or adaptive immunity compared to C57BL/6 (B6) controls. In contrast, immunoproteasome deficiency in neonatal TKO mice was associated with decreased survival and decreased lung gamma interferon (IFN-γ) expression compared to B6 controls, although without substantial effects on viral replication, histological evidence of inflammation, or expression of the proinflammatory cytokines tumor necrosis factor alpha and interleukin-1ß in lungs or other organs. T cell recruitment and IFN-γ production was similar in lungs of infected B6 and TKO mice. In lungs of uninfected B6 mice, we detected low levels of immunoproteasome subunit mRNA and protein that increased with age. Immunoproteasome subunit expression was lower in lungs of adult IFN-γ-deficient mice compared to B6 controls. Together, these results demonstrate developmental regulation of the immunoproteasome that is associated with age-dependent differences in MAV-1 pathogenesis.IMPORTANCE MAV-1 infection is a useful model to study the pathogenesis of an adenovirus in its natural host. Host factors that control MAV-1 replication and contribute to inflammation and disease are not fully understood. The immunoproteasome is an inducible component of the ubiquitin proteasome system that shapes the repertoire of peptides presented by MHC class I to CD8 T cells, influences other aspects of T cell survival and activation, and promotes production of proinflammatory cytokines. We found that immunoproteasome activity is dispensable in adult mice. However, immunoproteasome deficiency in neonatal mice increased mortality and impaired IFN-γ responses in the lungs. Baseline immunoproteasome subunit expression in lungs of uninfected mice increased with age. Our findings suggest the existence of developmental regulation of the immunoproteasome, like other aspects of host immune function, and indicate that immunoproteasome activity is a critical protective factor early in life.


Assuntos
Infecções por Adenoviridae/imunologia , Infecções por Adenoviridae/patologia , Fatores Etários , Linfócitos T CD8-Positivos/imunologia , Complexo de Endopeptidases do Proteassoma/metabolismo , Infecções Respiratórias/imunologia , Infecções Respiratórias/patologia , Animais , Modelos Animais de Doenças , Mastadenovirus/crescimento & desenvolvimento , Mastadenovirus/imunologia , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Complexo de Endopeptidases do Proteassoma/deficiência , Análise de Sobrevida , Replicação Viral
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