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1.
Int J Mol Sci ; 23(17)2022 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-36077230

RESUMO

The adenoviruses (AdVs) isolated from humans are taxonomically grouped in seven different species in the Mastadenovirus genus (HAdV-A through G). AdVs isolated from apes are often included in one of the human AdV species. Here we describe the sequence analyses of ten new AdVs that are related to the HAdV-C species and that were isolated from healthy western lowland gorillas, bonobos, chimpanzees, and orangutans kept in Dutch zoos. We analyzed these viruses and compared their genome sequences to those of human- and ape-derived AdV sequences in the NCBI GenBank database. Our data demonstrated that the ape-derived viruses clustering to HAdV-C are markedly distinct from the human HAdV-C species in the size and nucleotide composition (%GC) of their genome, differ in the amino-acid sequence of AdV proteins, and have longer RGD-loops in their penton-base proteins. The viruses form three well-separated clades (the human, the gorilla, and the combined group of the bonobo and chimpanzee viruses), and we propose that these should each be given species-level ranks. The Ad-lumc005 AdV isolated from orangutans was found to be very similar to the gorilla AdVs, and bootstrap inference provided evidence of recombination between the orangutan AdV and the gorilla AdVs. This suggests that this virus may not be a genuine orangutan AdV but may have been transferred from a gorilla to an orangutan host.


Assuntos
Adenovírus Humanos , Hominidae , Mastadenovirus , Adenoviridae/genética , Adenovírus Humanos/genética , Animais , Gorilla gorilla , Hominidae/genética , Humanos , Pan troglodytes , Filogenia , Pongo
2.
Braz J Microbiol ; 53(4): 2101-2105, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36104574

RESUMO

The Adenoviridae family is composed by a high diversity of viruses that are extremely resistant in environment and are frequently excreted in animal reservoir feces for long periods. The knowledge of adenovirus (AdV) diversity among wild species may be important for the understanding of the epidemiology of putative emerging diseases. Cavia aperea aperea, commonly known as wild guinea pigs, wild cavies, or preas, are small herbivorous rodents widely distributed throughout South America and classified in Caviidae family, as well as domestic guinea pigs and capybaras. In order to investigate their potential role as reservoir of zoonotic agents, the present study aimed to verify the presence of AdV in fecal samples of 14 preas from Northeast Brazil. When submitted to nested PCR, two out of 14 samples (14.28%) were positive for AdV and classified as human Mastadenovirus C (HAdV-C) using DNA sequencing and phylogenetic analysis. Wild guinea pigs are synanthropic rodents that live in close contact with humans. The investigation of viral agents in rodents is important due to their potential role as reservoirs of human and animal pathogens. Moreover, the present work presents the first known evidence of HAdV in wild guinea pig stool samples, which may represent both the impact of anthropogenic pollution to wild animals and an important knowledge in terms of human health.


Assuntos
Animais Selvagens , Mastadenovirus , Humanos , Cobaias , Animais , Filogenia , Fezes , Análise de Sequência de DNA , Mastadenovirus/genética
3.
Braz J Microbiol ; 53(3): 1465-1471, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-35666431

RESUMO

Molecular methodologies providing data on viral concentration and infectivity have been successfully used in environmental virology, supporting quantitative risk assessment studies. The present study aimed to assess human mastadenovirus (HAdV) intact particles using a derivative of propidium monoazide associated with qPCR (PMAxx-qPCR) in aquatic matrices. Initially, different concentrations of PMAxx were evaluated to establish an optimal protocol for treating different naturally contaminated matrices, using 10 min incubation in the dark at 200 rpm at room temperature and 15 min of photoactivation in the PMA-Lite™ LED photolysis device. There was no significant reduction in the quantification of infectious HAdV with increasing concentration of PMAxx used (20 µM, 50 µM, and 100 µM), except for sewage samples. In this matrix, a reduction of 5.01 log of genomic copies (GC)/L was observed from the concentration of 50 µM and revealed 100% HAdV particles with damaged capsids. On the other hand, the mean reduction of 0.51 log in stool samples using the same concentration mentioned above demonstrated 83% of damaged particles eliminated in the stool. Following, 50 µM PMAxx-qPCR protocol revealed a log reduction of 0.91, 0.67, and 1.05 in other samples of raw sewage, brackish, and seawater where HAdV concentration reached 1.47 × 104, 6.81 × 102, and 2.33 × 102 GC/L, respectively. Fifty micrometers of PMAxx protocol helped screen intact viruses from different matrices, including sea and brackish water.


Assuntos
Mastadenovirus , Esgotos , Humanos , Reação em Cadeia da Polimerase em Tempo Real/métodos , Água do Mar
4.
J Mol Biol ; 434(10): 167563, 2022 05 30.
Artigo em Inglês | MEDLINE | ID: mdl-35351519

RESUMO

Over one hundred Mastadenovirus types infect seven orders of mammals. Virus-host coevolution may involve cospeciation, duplication, host switch and partial extinction events. We reconstruct Mastadenovirus diversification, finding that while cospeciation is dominant, the other three events are also common in Mastadenovirus evolution. Linear motifs are fast-evolving protein functional elements and key mediators of virus-host interactions, thus likely to partake in adaptive viral evolution. We study the evolution of eleven linear motifs in the Mastadenovirus E1A protein, a hub of virus-host protein-protein interactions, in the context of host diversification. The reconstruction of linear motif gain and loss events shows fast linear motif turnover, corresponding a virus-host protein-protein interaction turnover orders of magnitude faster than in model host proteomes. Evolution of E1A linear motifs is coupled, indicating functional coordination at the protein scale, yet presents motif-specific patterns suggestive of convergent evolution. We report a pervasive association between Mastadenovirus host diversification events and the evolution of E1A linear motifs. Eight of 17 host switches associate with the gain of one linear motif and the loss of four different linear motifs, while five of nine partial extinctions associate with the loss of one linear motif. The specific changes in E1A linear motifs during a host switch or a partial extinction suggest that changes in the host molecular environment lead to modulation of the interactions with the retinoblastoma protein and host transcriptional regulators. Altogether, changes in the linear motif repertoire of a viral hub protein are associated with adaptive evolution events during Mastadenovirus evolution.


Assuntos
Proteínas E1A de Adenovirus , Evolução Molecular , Interações Hospedeiro-Patógeno , Mastadenovirus , Proteínas E1A de Adenovirus/química , Proteínas E1A de Adenovirus/genética , Motivos de Aminoácidos , Animais , Mamíferos/virologia , Mastadenovirus/química , Mastadenovirus/genética , Mapeamento de Interação de Proteínas
5.
Microbiol Spectr ; 10(1): e0156921, 2022 02 23.
Artigo em Inglês | MEDLINE | ID: mdl-35171015

RESUMO

Human mastadenovirus (HAdV), a linear double-stranded DNA (dsDNA) virus, is the causal agent of several diseases, including pharyngoconjunctival fever, epidemic keratoconjunctivitis, and hemorrhagic cystitis, in immunocompromised individuals. There are more than 100 reported types of adenoviruses, but the pathogenicity of many HAdVs remains unknown. Brincidofovir (BCV) is a hexadecyloxypropyl lipid conjugate of cidofovir (CDV) that is active against dsDNA viruses. Clinical effectiveness of BCV against certain HAdV species has been reported; however, its activity against novel HAdV types remains unknown. We investigated the half-maximal inhibitory concentration (IC50) values of BCV for novel HAdV types and found that the epidemic keratoconjunctivitis-associated HAdV-D54 prevalent in the Asian region was the most susceptible. The mean overall IC50 value of BCV was lower than that of CDV, indicating that BCV is effective against HAdVs, including the novel types. IMPORTANCE We investigated the IC50 values of BCV for novel HAdV types and found that the epidemic keratoconjunctivitis-associated HAdV-D54 prevalent in the Asian region was the most susceptible. In addition, the mean overall IC50 value of BCV was lower than that of CDV, indicating that BCV is effective against HAdVs.


Assuntos
Infecções por Adenoviridae/virologia , Infecções por Adenovirus Humanos/virologia , Citosina/análogos & derivados , Ceratoconjuntivite/virologia , Mastadenovirus/efeitos dos fármacos , Organofosfonatos/farmacologia , Infecções por Adenoviridae/imunologia , Infecções por Adenovirus Humanos/imunologia , Cistite , Citosina/farmacologia , Humanos , Hospedeiro Imunocomprometido , Ceratoconjuntivite/imunologia , Mastadenovirus/classificação , Mastadenovirus/genética , Mastadenovirus/fisiologia
6.
Arch Virol ; 167(3): 979-982, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-35112204

RESUMO

Here, we report a novel bat adenovirus strain isolated from apparently healthy bats of the species Rhinolophus cornutus in Japan. The genome of the isolate was 36,506 bp in length and encoded at least 33 proteins. Phylogenetic analysis of the DNA polymerase amino acid sequence, which provides one demarcation criterion for adenoviral species, indicated that the isolate belongs to the species Bat mastadenovirus C in the genus Mastadenovirus. Most of the encoded proteins shared high sequence similarity with those of known bat adenovirus C strains detected in different species of Rhinolophus, whereas the fiber protein and some E3- and E4-related proteins shared moderate similarity, and only the large E3 protein, which contains several host immune-suppression-related motifs, showed considerably lower similarity.


Assuntos
Quirópteros , Mastadenovirus , Animais , Genoma Viral , Japão , Mastadenovirus/genética , Filogenia
7.
Virus Res ; 308: 198653, 2022 01 15.
Artigo em Inglês | MEDLINE | ID: mdl-34896154

RESUMO

Bats are important reservoirs for many kinds of emerging zoonotic viruses. In order to explore potential pathogens carried by bats and trace the source of adenovirus outbreaks on the southeastern coast of China, we took pharyngeal and anal swabs from a total of 552 bats (Rhinolophus pusillus) collected from various areas of Chinese southeastern coast. Adenoviruses were identified in 36 out of the 552 samples (6.5%) . Complete genome sequences of two adenovirus isolations from Vero E6 cells were obtained, which were further validated as identical strains via next-generation sequencing and were named Bat-Advcxc6. The cell culture inoculated with the two samples exhibited remarkable cytopathic changes. The full genome has 37,315 bp and owns 29 open reading frames. Phylogenetic analyses confirmed that Bat-Advcxc6 represented a novel bat adenovirus species in the genus Mastadenovirus. Transmission electron microgram showed clear virus particles. Bat-Advcxc6 shared similar characteristics of G + C contents with Bat mastadenovirus WIV11 (Bat mastadenovirus C) found in China in 2016, but differed from this serotype due to a <75% similarity with DNA polymerase amino acid sequences in WIV11. As it is a newly found adenovirus strain according to the international classification criteria, further analyses of virus dynamics, epithelial invasion, and immunization assays are required to explore its potential threats of cross-species transmission.


Assuntos
Infecções por Adenoviridae , Quirópteros , Mastadenovirus , Adenoviridae/genética , Infecções por Adenoviridae/epidemiologia , Animais , China , Genoma Viral , Filogenia , Virulência
8.
Viruses ; 13(11)2021 10 30.
Artigo em Inglês | MEDLINE | ID: mdl-34835000

RESUMO

Using a broad-range nested PCR assay targeting the DNA-dependent DNA polymerase (pol) gene, we detected adenoviruses in 17 (20.48%) out of 83 fecal samples from small Indian mongooses (Urva auropunctata) on the Caribbean island of St. Kitts. All 17 PCR amplicons were sequenced for the partial pol gene (~300 bp, hereafter referred to as Mon sequences). Fourteen of the 17 Mon sequences shared maximum homology (98.3-99.6% and 97-98.9% nucleotide (nt) and deduced amino acid (aa) sequence identities, respectively) with that of bovine adenovirus-6 (species Bovine atadenovirus E). Mongoose-associated adenovirus Mon-39 was most closely related (absolute nt and deduced aa identities) to an atadenovirus from a tropical screech owl. Mon-66 shared maximum nt and deduced aa identities of 69% and 71.4% with those of atadenoviruses from a spur-thighed tortoise and a brown anole lizard, respectively. Phylogenetically, Mon-39 and Mon-66 clustered within clades that were predominated by atadenoviruses from reptiles, indicating a reptilian origin of these viruses. Only a single mongoose-associated adenovirus, Mon-34, was related to the genus Mastadenovirus. However, phylogenetically, Mon-34 formed an isolated branch, distinct from other mastadenoviruses. Since the fecal samples were collected from apparently healthy mongooses, we could not determine whether the mongoose-associated adenoviruses infected the host. On the other hand, the phylogenetic clustering patterns of the mongoose-associated atadenoviruses pointed more towards a dietary origin of these viruses. Although the present study was based on partial pol sequences (~90 aa), sequence identities and phylogenetic analysis suggested that Mon-34, Mon-39, and Mon-66 might represent novel adenoviruses. To our knowledge, this is the first report on the detection and molecular characterization of adenoviruses from the mongoose.


Assuntos
Adenoviridae/classificação , Adenoviridae/genética , Adenoviridae/isolamento & purificação , Herpestidae/virologia , Infecções por Adenoviridae/veterinária , Infecções por Adenoviridae/virologia , Sequência de Aminoácidos , Animais , Atadenovirus/classificação , Atadenovirus/genética , Atadenovirus/isolamento & purificação , DNA Polimerase Dirigida por DNA , Fezes/virologia , Lagartos/virologia , Mastadenovirus/classificação , Mastadenovirus/genética , Mastadenovirus/isolamento & purificação , Filogenia , Reação em Cadeia da Polimerase , Tartarugas/virologia , Índias Ocidentais
9.
Sci Rep ; 11(1): 16706, 2021 08 18.
Artigo em Inglês | MEDLINE | ID: mdl-34408176

RESUMO

Although a canine adenovirus (CAdV)-based oncolytic virus (OV) candidate targeting canine tumors has been reported, its oncolytic effect could be attenuated by CAdV vaccine-induced neutralizing antibodies in dog patients. To circumvent this issue, we focused on the bat adenovirus (BtAdV) strain, which was previously isolated from healthy microbats. We previously showed that this virus replicated efficiently in canine cell lines and did not serologically cross-react with CAdVs, suggesting that it may offer the possibility of an OV candidate for canine tumors. Here, we tested the growth properties and cytotoxicity of the BtAdV Mm32 strain in a panel of canine tumor cells and found that its characteristics were equivalent to those of CAdVs. To produce an Mm32 construct with enhanced tumor specificity, we established a novel reverse genetics system for BtAdV based on bacterial artificial chromosomes, and generated a recombinant virus, Mm32-E1Ap + cTERTp, by inserting a tumor-specific canine telomerase reverse transcriptase promoter into its E1A regulatory region. The growth and cytotoxicity of this recombinant were superior to those of wild-type Mm32 in canine tumor cells, unlike in normal canine cells. These data suggest that Mm32-E1Ap + cTERTp could be a promising OV for alternative canine cancer therapies.


Assuntos
Quirópteros/virologia , Doenças do Cão/terapia , Mastadenovirus , Neoplasias , Terapia Viral Oncolítica , Vírus Oncolíticos , Animais , Linhagem Celular Tumoral , Cromossomos Artificiais Bacterianos/genética , Cães , Células Madin Darby de Rim Canino , Mastadenovirus/genética , Mastadenovirus/metabolismo , Neoplasias/terapia , Neoplasias/veterinária , Vírus Oncolíticos/genética , Vírus Oncolíticos/metabolismo
10.
J Med Virol ; 93(7): 4392-4398, 2021 07.
Artigo em Inglês | MEDLINE | ID: mdl-33829531

RESUMO

With the arrival of coronavirus disease 2019 (COVID-19) in Brazil in February 2020, several preventive measures were taken by the population aiming to avoid severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection including the use of masks, social distancing, and frequent hand washing then, these measures may have contributed to preventing infection also by other respiratory viruses. Our goal was to determine the frequencies of Influenza A and B viruses (FLUAV/FLUBV), human mastadenovirus C (HAdV-C), Enterovirus 68 (EV-68), and rhinovirus (RV) besides SARS-CoV-2 among hospitalized patients suspect of COVID-19 with cases of acute respiratory disease syndrome (ARDS) in the period of March to December 2020 and to detect possible coinfections among them. Nucleic acid detection was performed using reverse-transcription quantitative polymerase chain reaction (RT-qPCR) in respiratory samples using naso-oropharyngeal swabs and bronchoalveolar lavage. A total of 418 samples of the 987 analyzed (42.3%) were positive for SARS-CoV-2, 16 (1.62%) samples were positive for FLUAV, no sample was positive for FLUBV or EV-68, 67 (6.78%) samples were positive for HAdV-C, 55 samples were positive for RV 1/2 (26.3%) and 37 for RV 2/2 (13.6%). Coinfections were also detected, including a triple coinfection with SARS-CoV-2, FLUAV, and HAdV-C. In the present work, a very low frequency of FLUV was reported among hospitalized patients with ARDS compared to the past years, probably due to preventive measures taken to avoid COVID-19 and the high influenza vaccination coverage in the region in which this study was performed.


Assuntos
Infecções por Adenoviridae/epidemiologia , COVID-19/epidemiologia , Resfriado Comum/epidemiologia , Infecções por Enterovirus/epidemiologia , Influenza Humana/epidemiologia , Distanciamento Físico , Infecções por Adenoviridae/prevenção & controle , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Brasil/epidemiologia , COVID-19/prevenção & controle , Criança , Pré-Escolar , Coinfecção/epidemiologia , Coinfecção/virologia , Resfriado Comum/prevenção & controle , Enterovirus Humano D/genética , Enterovirus Humano D/isolamento & purificação , Infecções por Enterovirus/prevenção & controle , Feminino , Humanos , Lactente , Vírus da Influenza A/genética , Vírus da Influenza A/isolamento & purificação , Vírus da Influenza B/genética , Vírus da Influenza B/isolamento & purificação , Influenza Humana/prevenção & controle , Masculino , Máscaras , Mastadenovirus/genética , Mastadenovirus/isolamento & purificação , Pessoa de Meia-Idade , Técnicas de Amplificação de Ácido Nucleico/métodos , Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos , Rhinovirus/genética , Rhinovirus/isolamento & purificação , SARS-CoV-2/genética , SARS-CoV-2/isolamento & purificação , Adulto Jovem
11.
Sci Rep ; 11(1): 6331, 2021 03 18.
Artigo em Inglês | MEDLINE | ID: mdl-33737691

RESUMO

Few publications, often limited to one specific pathogen, have studied bonobos (Pan paniscus), our closest living relatives, as possible reservoirs of certain human infectious agents. Here, 91 stool samples from semicaptive bonobos and bonobos reintroduced in the wild, in the Democratic Republic of the Congo, were screened for different infectious agents: viruses, bacteria and parasites. We showed the presence of potentially zoonotic viral, bacterial or parasitic agents in stool samples, sometimes coinfecting the same individuals. A high prevalence of Human mastadenoviruses (HAdV-C, HAdV-B, HAdV-E) was observed. Encephalomyocarditis viruses were identified in semicaptive bonobos, although identified genotypes were different from those identified in the previous fatal myocarditis epidemic at the same site in 2009. Non-pallidum Treponema spp. including symbiotic T. succinifaciens, T. berlinense and several potential new species with unknown pathogenicity were identified. We detected DNA of non-tuberculosis Mycobacterium spp., Acinetobacter spp., Salmonella spp. as well as pathogenic Leptospira interrogans. Zoonotic parasites such as Taenia solium and Strongyloides stercoralis were predominantly present in wild bonobos, while Giardia lamblia was found only in bonobos in contact with humans, suggesting a possible exchange. One third of bonobos carried Oesophagostomum spp., particularly zoonotic O. stephanostomum and O. bifurcum-like species, as well as other uncharacterized Nematoda. Trypanosoma theileri has been identified in semicaptive bonobos. Pathogens typically known to be transmitted sexually were not identified. We present here the results of a reasonably-sized screening study detecting DNA/RNA sequence evidence of potentially pathogenic viruses and microorganisms in bonobo based on a noninvasive sampling method (feces) and focused PCR diagnostics.


Assuntos
Espécies em Perigo de Extinção , Interações Hospedeiro-Patógeno/genética , Mastadenovirus/isolamento & purificação , Pan paniscus/virologia , Animais , República Democrática do Congo/epidemiologia , Vírus da Encefalomiocardite/isolamento & purificação , Vírus da Encefalomiocardite/patogenicidade , Fezes/microbiologia , Fezes/parasitologia , Fezes/virologia , Humanos , Mastadenovirus/patogenicidade , Pan paniscus/microbiologia , Pan paniscus/parasitologia , Pan troglodytes/microbiologia , Pan troglodytes/parasitologia , Pan troglodytes/virologia , Parasitos/isolamento & purificação , Parasitos/patogenicidade
12.
Res Vet Sci ; 135: 450-455, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33203584

RESUMO

BRD is associated with infectious agents, but management and transport-stress are trigger factors. Metaphylactic administration of antimicrobial reduces colonization of respiratory tract by pathogens, but the development of antibiotic-resistance raises public health concerns leading to propose new control strategies. The study analyzed nasopharyngeal swabs of 231 imported cattle, 10% of 49 trucks, transported from France to southern Italy and, through Real-time PCR identified the prevalence of the involved pathogens speculating on strategies to reduce the impact of BRD. The samples were tested by Real-time PCR, for the detection of bovine coronavirus (BCoV), bovine respiratory syncytial virus (BRSV), bovine parainfluenza virus (BPiV), bovine adenovirus (BAdV), Mannheimia haemolytica, Pasteurella multocida, Histophilus somni, and Mycoplasma bovis. Yates-corrected chi squared, or Fisher's exact test were used to compare both animal-health status and positivity/negativity to pathogens, and the relationship between presence/absence of clinical signs and Real-time PCR-positivity. H. somni and BCoV were the most frequently identified pathogens. In BRD-diagnosed cattle, BAdV was detected in 13.8% (19/138), BRSV in 14.5% (20/138) and BPiV in 4.3% (6/138). Healthy cattle were mostly positive for H. somni (89.2%, 83/93). A statistically significant association was observed between clinical signs and positivity to M. haemolytica (p value = 0.016). Although mass-medication and vaccination are used for BRD control, it still remains a primary health problem. Our results highlight that the nasopharyngeal microbiota could be affected by transport and that strategies to enhance calf immunity for reducing BRD-risk development would be more effective if applied at farm of origin prior to loading.


Assuntos
Doenças dos Bovinos/epidemiologia , Coronavirus Bovino/isolamento & purificação , Microbiota , Pasteurellaceae/isolamento & purificação , Doenças Respiratórias/veterinária , Animais , Bovinos , Doenças dos Bovinos/microbiologia , Doenças dos Bovinos/prevenção & controle , Coronavirus Bovino/genética , Estudos Epidemiológicos , França/epidemiologia , Imunidade , Itália/epidemiologia , Masculino , Mastadenovirus/genética , Mastadenovirus/isolamento & purificação , Nasofaringe/microbiologia , Pasteurellaceae/genética , Vírus Sincicial Respiratório Bovino/genética , Vírus Sincicial Respiratório Bovino/isolamento & purificação , Sistema Respiratório/microbiologia , Doenças Respiratórias/epidemiologia , Doenças Respiratórias/microbiologia , Doenças Respiratórias/prevenção & controle , Respirovirus/genética , Respirovirus/isolamento & purificação , Meios de Transporte
13.
Viruses ; 12(12)2020 12 12.
Artigo em Inglês | MEDLINE | ID: mdl-33322850

RESUMO

A number of characteristics including lack of virulence and the ability to grow to high titers, have made bovine adenovirus-3 (BAdV-3) a vector of choice for further development as a vaccine-delivery vehicle for cattle. Despite the importance of blood leukocytes, including dendritic cells (DC), in the induction of protective immune responses, little is known about the interaction between BAdV-3 and bovine blood leukocytes. Here, we demonstrate that compared to other leukocytes, bovine blood monocytes and neutrophils are significantly transduced by BAdV404a (BAdV-3, expressing enhanced yellow green fluorescent protein [EYFP]) at a MOI of 1-5 without a significant difference in the mean fluorescence of EYFP expression. Moreover, though expression of some BAdV-3-specific proteins was observed, no progeny virions were detected in the transduced monocytes or neutrophils. Interestingly, addition of the "RGD" motif at the C-terminus of BAdV-3 minor capsid protein pIX (BAV888) enhanced the ability of the virus to enter the monocytes without altering the tropism of BAdV-3. The increased uptake of BAV888 by monocytes was associated with a significant increase in viral genome copies and the abundance of EYFP and BAdV-3 19K transcripts compared to BAdV404a-transduced monocytes. Our results suggest that BAdV-3 efficiently transduces monocytes and neutrophils in the absence of viral replication. Moreover, RGD-modified capsid significantly increases vector uptake without affecting the initial interaction with monocytes.


Assuntos
Infecções por Adenoviridae/veterinária , Doenças dos Bovinos/virologia , Leucócitos/virologia , Mastadenovirus/fisiologia , Tropismo Viral , Animais , Bovinos , Doenças dos Bovinos/imunologia , Doenças dos Bovinos/metabolismo , Linhagem Celular , Expressão Gênica , Regulação Viral da Expressão Gênica , Leucócitos/imunologia , Leucócitos/metabolismo , Transdução Genética , Proteínas Virais/genética , Proteínas Virais/metabolismo , Replicação Viral
14.
Acta Vet Hung ; 68(3): 323-327, 2020 09 30.
Artigo em Inglês | MEDLINE | ID: mdl-33128521

RESUMO

The complete genomic sequence along with phylogenetic analyses of an adenovirus (AdV), isolated from a dead captive pygmy marmoset (Callithrix pygmaea) from a Hungarian zoo is reported. Earlier, based on the phylogenetic analysis of the sequence of a PCR-amplified fragment from the DNA polymerase gene, the pygmy marmoset AdV (PMAdV) has been reported to cluster closest to certain chiropteran AdVs. In the following years similar AdVs were discovered in additional mammalian hosts, including a skunk (Mephitis mephitis), African pygmy hedgehogs (Atelerix albiventris), North American porcupines (Erethizon dorsatum) and grey fox (Urocyon cinereoargenteus). After the full genome analysis of the skunk adenovirus (SkAdV-1), a novel species Skunk mastadenovirus A (SkAdV-A) has been established. The AdVs, originating from the African pygmy hedgehogs, have been found to belong to virus species SkAdV-A. Partial gene sequences from the porcupine AdVs have also implied their very close genetic relatedness to SkAdV-A. The complete genomic sequence of PMAdV, examined in this study, was found to share 99.83% nucleotide identity with SkAdV-1, thus unequivocally represents a genomic variant of SkAdV-1. The observation that viruses classifiable as SkAdV-A are able to infect and cause diseases in several, distantly related mammals seems to deserve further studies to elucidate the infection biology of this intriguing AdV.


Assuntos
Callithrix/virologia , Genoma Viral , Mastadenovirus/genética , Mephitidae/virologia , Animais , Mastadenovirus/classificação , Sequenciamento Completo do Genoma/veterinária
15.
Viruses ; 12(6)2020 06 18.
Artigo em Inglês | MEDLINE | ID: mdl-32570742

RESUMO

Non-human primates (NHPs) are known hosts for adenoviruses (AdVs), so there is the possibility of the zoonotic or cross-species transmission of AdVs. As with humans, AdV infections in animals can cause diseases that range from asymptomatic to fatal. The aim of this study was to investigate the occurrence and diversity of AdVs in: (i) fecal samples of apes and monkeys from different African countries (Republic of Congo, Senegal, Djibouti and Algeria), (ii) stool of humans living near gorillas in the Republic of Congo, in order to explore the potential zoonotic risks. Samples were screened by real-time and standard PCRs, followed by the sequencing of the partial DNA polymerase gene in order to identify the AdV species. The prevalence was 3.3 folds higher in NHPs than in humans. More than 1/3 (35.8%) of the NHPs and 1/10 (10.5%) of the humans excreted AdVs in their feces. The positive rate was high in great apes (46%), with a maximum of 54.2% in chimpanzees (Pan troglodytes) and 35.9% in gorillas (Gorilla gorilla), followed by monkeys (25.6%), with 27.5% in Barbary macaques (Macaca sylvanus) and 23.1% in baboons (seven Papio papio and six Papio hamadryas). No green monkeys (Chlorocebus sabaeus) were found to be positive for AdVs. The AdVs detected in NHPs were members of Human mastadenovirus E (HAdV-E), HAdV-C or HAdV-B, and those in the humans belonged to HAdV-C or HAdV-D. HAdV-C members were detected in both gorillas and humans, with evidence of zoonotic transmission since phylogenetic analysis revealed that gorilla AdVs belonging to HAdV-C were genetically identical to strains detected in humans who had been living around gorillas, and, inversely, a HAdV-C member HAdV type was detected in gorillas. This confirms the gorilla-to-human transmission of adenovirus. which has been reported previously. In addition, HAdV-E members, the most often detected here, are widely distributed among NHP species regardless of their origin, i.e., HAdV-E members seem to lack host specificity. Virus isolation was successful from a human sample and the strain of the Mbo024 genome, of 35 kb, that was identified as belonging to HAdV-D, exhibited close identity to HAdV-D members for all genes. This study provides information on the AdVs that infect African NHPs and the human populations living nearby, with an evident zoonotic transmission. It is likely that AdVs crossed the species barrier between different NHP species (especially HAdV-E members), between NHPs and humans (especially HAdV-C), but also between humans, NHPs and other animal species.


Assuntos
Infecções por Adenoviridae/epidemiologia , Infecções por Adenoviridae/veterinária , Mastadenovirus/classificação , Mastadenovirus/isolamento & purificação , Infecções por Adenoviridae/transmissão , Argélia/epidemiologia , Animais , Chlorocebus aethiops/virologia , Congo/epidemiologia , DNA Viral/genética , DNA Polimerase Dirigida por DNA/genética , Djibuti/epidemiologia , Fezes/virologia , Gorilla gorilla/virologia , Humanos , Macaca/virologia , Mastadenovirus/genética , Pan troglodytes/virologia , Papio hamadryas/virologia , Papio papio/virologia , Senegal/epidemiologia , Zoonoses Virais/epidemiologia , Zoonoses Virais/transmissão
16.
Virology ; 547: 12-19, 2020 08.
Artigo em Inglês | MEDLINE | ID: mdl-32560900

RESUMO

CD8 T cells contribute to effective clearance of mouse adenovirus type 1 (MAV-1) and to virus-induced pulmonary inflammation. We characterized effects of a CD8 T cell effector, TNF, on MAV-1 pathogenesis. TNF inhibited MAV-1 replication in vitro. TNF deficiency or immunoneutralization had no effect on lung viral loads or viral gene expression in mice infected intranasally with MAV-1. Absence of TNF delayed virus-induced weight loss and reduced histological evidence of pulmonary inflammation, although concentrations of proinflammatory cytokines and chemokines in bronchoalveolar lavage fluid (BALF) were not significantly affected. BALF concentrations of IL-10 were greater in TNF-deficient mice compared to controls. Our data indicate that TNF is not essential for control of viral replication in vivo, but virus-induced TNF contributes to some aspects of immunopathology and disease. Redundant CD8 T cell effectors and other aspects of immune function are sufficient for antiviral and pro-inflammatory responses to acute MAV-1 respiratory infection.


Assuntos
Infecções por Adenoviridae/virologia , Mastadenovirus/fisiologia , Pneumonia/imunologia , Fator de Necrose Tumoral alfa/imunologia , Replicação Viral , Infecções por Adenoviridae/genética , Infecções por Adenoviridae/imunologia , Animais , Linfócitos T CD8-Positivos/imunologia , Humanos , Interleucina-10/genética , Interleucina-10/imunologia , Pulmão/imunologia , Pulmão/virologia , Masculino , Mastadenovirus/genética , Camundongos , Camundongos Endogâmicos C57BL , Pneumonia/genética , Pneumonia/virologia , Fator de Necrose Tumoral alfa/genética
17.
Infect Genet Evol ; 83: 104348, 2020 09.
Artigo em Inglês | MEDLINE | ID: mdl-32380313

RESUMO

Bovine adenovirus type 3 (BAdV-3) is an important pathogen causing bovine respiratory disease complex (BRDC). From Jun 2016 to Jun 2018, 108 nose swab samples were collected from cattle with BRDC from 11 farms in five cities, and 78.7% (85/108) samples were detected as BAdV-3 positive by Real-time PCR. Interestingly, the sequences of 7/10 fiber (852 bp) and hexon (785 bp) fragments cloned from 10 positive samples from eight farms were clustered into a single branch of the evolutionary tree. A BAdV-3 strain (BO/YB24/17/CH) was successfully isolated. The isolate caused pathological changes of lung, trachea and spleen in BALB/c mice. Notably, 79 amino acid deletions in the shaft domain and 74 unique amino acid mutations were found in the fiber gene of the isolate compared with the available complete sequences for fiber genes in the GenBank database. These characteristics indicated that the isolate may represent a novel fiber genotype of BAdV-3. A pair of specific primers covering the deletion region in the fiber gene was designed to screen the prevalence of BAdV-3 encoding the novel fiber gene. The results showed that 7 of the 10 strains possessed the novel fiber gene, and these novel fiber strains were detected from six farms in which calves were just imported from five provinces, indicating that this BAdV-3 with the natural deletion fiber gene has a wide geographical distribution in China. In conclusion, our results reveal that BAdV-3 is widespread in China and a pathogenic BAdV-3 strain with a novel fiber gene has been detected at high frequency, which is beneficial to understand the prevalence and genetic evolution of BAdV-3.


Assuntos
Infecções por Adenoviridae/virologia , Doenças dos Bovinos/virologia , Mastadenovirus/genética , Proteínas Virais/genética , Infecções por Adenoviridae/epidemiologia , Infecções por Adenoviridae/veterinária , Animais , Bovinos , Doenças dos Bovinos/epidemiologia , China/epidemiologia , Deleção de Genes , Mastadenovirus/isolamento & purificação , Mastadenovirus/patogenicidade , Camundongos Endogâmicos BALB C , Epidemiologia Molecular , Filogenia , Prevalência , Reação em Cadeia da Polimerase em Tempo Real
18.
Virology ; 546: 25-37, 2020 07.
Artigo em Inglês | MEDLINE | ID: mdl-32452415

RESUMO

Bovine adenovirus-3 (BAdV-3) is a non enveloped, icosahedral DNA virus containing a genome of 34446 bps. The intermediate region of BAdV-3 encodes pIX and IVa2 proteins. Here, we report the characterization of BAdV-3 IVa2. Anti-IVa2 serum detected a 50 kDa protein at 24-48 h post infection in BAdV-3 infected cells. The IVa2 localizes to nucleus and nucleolus of BAdV-3 infected cells. Analysis of mutant IVa2 demonstrated that amino acids 1-25 and 373-448 are required for nuclear and nucleolar localization of IVa2, respectively. The nuclear import of IVa2 utilize importin α -1 of importin nuclear import pathway. Although deletion/substitution of amino acids 4-18 is sufficient to abrogate the nuclear localization of IVa2, amino acids 1-25 are required for nuclear localization of a cytoplasmic protein. Furthermore, we demonstrate that amino acids 1-25 and 120-140 of IVa2 interact with importin α-1 and pV proteins, respectively in BAdV-3 infected cells.


Assuntos
Infecções por Adenoviridae/veterinária , Doenças dos Bovinos/virologia , Nucléolo Celular/virologia , Mastadenovirus/metabolismo , Proteínas Virais/química , Proteínas Virais/metabolismo , Transporte Ativo do Núcleo Celular , Infecções por Adenoviridae/genética , Infecções por Adenoviridae/metabolismo , Infecções por Adenoviridae/virologia , Motivos de Aminoácidos , Animais , Bovinos , Doenças dos Bovinos/genética , Doenças dos Bovinos/metabolismo , Nucléolo Celular/genética , Nucléolo Celular/metabolismo , Núcleo Celular/genética , Núcleo Celular/metabolismo , Núcleo Celular/virologia , Genoma Viral , Carioferinas/genética , Carioferinas/metabolismo , Mastadenovirus/química , Mastadenovirus/genética , Ligação Proteica , Transporte Proteico , Proteínas Virais/genética
19.
Jpn J Infect Dis ; 73(5): 349-353, 2020 Sep 24.
Artigo em Inglês | MEDLINE | ID: mdl-32350225

RESUMO

Seven human mastadenovirus (HAdV) species (A-G) are known with more than 100 reported types. HAdV is highly resistant to common hand sanitizers. Epidemic keratoconjunctivitis and pharyngoconjunctival fever are caused by HAdV, which can be explosively transmitted in a confined space, resulting in outbreaks, such as nosocomial infections. Given the absence of an antiviral agent against the HAdV infection, it is important to prevent the spread of the infection by using disinfectants. Ozone has already been well-known for its bactericidal and virucidal effects. ALTANT is an ozonated alcohol preparation developed by E-TECH Co., Ltd. (Kobe, Hyogo, Japan). In this study, we mixed ALTANT with different HAdV types at a ratio of 9:1 and determined HAdV viability after instantaneous reactions for varying periods (flash to 5 minutes) using the TCID50 assay. The assay results demonstrated that the HAdV viability decreased by 1/10 to 1/100 within 1 minute after the reaction; additionally, slight differences in the reactivity were observed among the HAdV types. HAdV viability decreased by a factor of > 4log10, and the virus was eliminated within 3 minutes. This study demonstrated the potent HAdV disinfection effect of ALTANT.


Assuntos
Infecções por Adenovirus Humanos/prevenção & controle , Desinfetantes/farmacologia , Etanol/farmacologia , Mastadenovirus/efeitos dos fármacos , Ozônio/farmacologia , Infecções por Adenovirus Humanos/virologia , Adenovírus Humanos , Anti-Infecciosos Locais/química , Anti-Infecciosos Locais/farmacologia , Proliferação de Células/efeitos dos fármacos , Infecção Hospitalar/prevenção & controle , Infecção Hospitalar/virologia , Surtos de Doenças , Desinfetantes/química , Etanol/química , Humanos , Japão , Ceratoconjuntivite/prevenção & controle , Ceratoconjuntivite/virologia , Mastadenovirus/patogenicidade , Testes de Sensibilidade Microbiana , Ozônio/química
20.
Virol Sin ; 35(5): 528-537, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32236817

RESUMO

Porcine adenoviruses (PAdVs) are classified into three species, PAdV-A, PAdV-B, and PAdV-C. The genomes of PAdV-A and PAdV-C have been well characterized. However, the genome of PAdV-B has never been completely sequenced, and the epidemiology of PAdV-B remains unclear. In our study, we have identified a novel strain of PAdV-B, named PAdV-B-HNU1, in porcine samples collected in China by viral metagenomic assay and general PCR. The genome of PAdV-B-HNU1 is 31,743 bp in length and highly similar to that of California sea lion adenovirus 1 (C. sea lion AdV-1), which contains typical mastadenoviral structures and some unique regions at the carboxy-terminal end. Especially, PAdV-B-HNU1 harbors a dUTPase coding region not clustering with other mastadenoviruses except for C. sea lion AdV-1 and a fiber coding region homologous with galectin 4 and 9 of animals. However, the variance of GC contents between PAdV-B-HNU1 (55%) and C. sea lion AdV-1 (36%) indicates their differential evolutionary paths. Further epidemiologic study revealed a high positive rate (51.7%) of PAdV-B-HNU1 in porcine lymph samples, but low positive rates of 10.2% and 16.1% in oral swabs and rectal swabs, respectively. In conclusion, this study characterized a novel representative genome of a lymphotropic PAdV-B with unique evolutionary origin, which contributes to the taxonomical and pathogenic studies of PAdVs.


Assuntos
Adenovirus Suínos , Mastadenovirus , Adenovirus Suínos/genética , Animais , Sequência de Bases , China , Genoma Viral , Mastadenovirus/genética , Fases de Leitura Aberta , Suínos
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