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1.
Sci Total Environ ; 805: 150288, 2022 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-34536868

RESUMO

Recovery of the nitrogen (N) and phosphorus (P) in wastewater would help to minimize eutrophication and their reuse would lead to a more sustainable society. Sewage sludge and fly ash were used to fabricate ceramsite in the laboratory. After modified with alkali or lanthanum it was shown in benchtop experiments to effectively recover N and P from real wastewater treatment plant effluent. The N&P-adsorbed ceramsite was then applied as an eco-friendly, slow-release fertilizer to promote the germination, growth and blooming of Impatiens commelinoides, realizing the recycling of N and P from wastewater. Emergy analysis shows that such recycling is more sustainable than the current two approaches (i.e., landfill and incineration) for sludge disposal. This work thus demonstrates a sustainable solution combining the reuse of solid waste, effective wastewater purification and recovery of N and P nutrients. Applying the technologies demonstrated would help to minimize the environmental impact of wastewater and solid waste.


Assuntos
Fósforo , Águas Residuárias , Adsorção , Misturas Complexas , Nitrogênio , Esgotos , Eliminação de Resíduos Líquidos
2.
BMC Oral Health ; 21(1): 498, 2021 10 06.
Artigo em Inglês | MEDLINE | ID: mdl-34615511

RESUMO

BACKGROUND: Dentin hypersensitivity (DH) is a sharp toothache that influences a patients' oral health-related quality of life. Oral dentifrices have been marketed for pain relief within a minute for DH. The permanent management of DH is being investigated with the remineralisation potential of bioactive agents in dentinal tubules. This study investigated the relief from pain in DH in one minute after applying over the counter (OTC) dentifrices with Pro-Argin™ and strontium acetate and directly compared them with fluoro-calcium phospho-silicate (FCPS)-based dentifrices for immediate and sustained inhibition of painful stimulus provoking DH. METHODS: A randomised, controlled, triple-blinded clinical trial was conducted with 140 participants clinically diagnosed with DH and equally randomized into four groups with parallel treatment assignment of FCPS, Pro-Argin™, 8% strontium acetate, and sodium fluoride-based OTC dentifrices, and tested for DH with air blast, mechanical, and water jet stimuli on SCHIFF cold air sensitivity scale (SCASS) and visual analogue scale (VAS) at interim efficacy intervals of one minute, three days, two, four, and six weeks, subsequently. RESULTS: A total of 128 participants completed the trial. All the treatment groups showed statistically significant improvement in DH with p < 0.001 relative to baseline at all time points. Pro-Argin™ showed a greater reduction in DH with mean scores of (1.34 ± 0.68) (4.20 ± 1.70) (3.05 ± 2.17) followed by strontium acetate (1.57 ± 0.81) (4.65 ± 1.87) (3.75 ± 1.97) on SCASS and VAS for mechanical and water jet stimuli, one minute after application. There was no statistically significant treatment difference between the two (p = 0.499). FCPS showed the highest reduction in DH on SCASS and VAS for waterjet stimuli with mean scores of (0.97 ± 0.68) (1.80 ± 1.73) and Pro-Argin™ on VAS for mechanical stimuli with mean scores of (2.15 ± 1.92) in six weeks. CONCLUSION: OTC dentifrices with Pro-argin™ and strontium acetate are effective for immediate pain relief from DH, and FCPS could be the best possible treatment option for long term management of DH. TRIAL REGISTRATION: ID: NCT04249336 ( https://clinicaltrials.gov/ct2/show/NCT04249336 ), Date of Registration: January 30, 2020 (Retrospectively registered).


Assuntos
Dentifrícios , Dessensibilizantes Dentinários , Sensibilidade da Dentina , Arginina , Misturas Complexas , Dentifrícios/uso terapêutico , Dessensibilizantes Dentinários/uso terapêutico , Sensibilidade da Dentina/tratamento farmacológico , Método Duplo-Cego , Combinação de Medicamentos , Fluoretos , Humanos , Nitratos , Fosfatos , Compostos de Potássio , Qualidade de Vida , Fluoreto de Sódio/uso terapêutico , Resultado do Tratamento
3.
Molecules ; 26(20)2021 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-34684861

RESUMO

Scientific investigation on essential oils composition and the related biological profile are continuously growing. Nevertheless, only a few studies have been performed on the relationships between chemical composition and biological data. Herein, the investigation of 61 assayed essential oils is reported focusing on their inhibition activity against Microsporum spp. including development of machine learning models with the aim of highlining the possible chemical components mainly related to the inhibitory potency. The application of machine learning and deep learning techniques for predictive and descriptive purposes have been applied successfully to many fields. Quantitative composition-activity relationships machine learning-based models were developed for the 61 essential oils tested as Microsporum spp. growth modulators. The models were built with in-house python scripts implementing data augmentation with the purpose of having a smoother flow between essential oils' chemical compositions and biological data. High statistical coefficient values (Accuracy, Matthews correlation coefficient and F1 score) were obtained and model inspection permitted to detect possible specific roles related to some components of essential oils' constituents. Robust machine learning models are far more useful tools to reveal data augmentation in comparison with raw data derived models. To the best of the authors knowledge this is the first report using data augmentation to highlight the role of complex mixture components, in particular a first application of these data will be for the development of ingredients in the dermo-cosmetic field investigating microbial species considering the urge for the use of natural preserving and acting antimicrobial agents.


Assuntos
Anti-Infecciosos/química , Aprendizado de Máquina , Microsporum/efeitos dos fármacos , Óleos Voláteis/química , Anti-Infecciosos/farmacologia , Arthrodermataceae/efeitos dos fármacos , Misturas Complexas/química , Misturas Complexas/farmacologia , Coleta de Dados , Óleos Voláteis/farmacologia , Filogenia , Relação Estrutura-Atividade
4.
Int J Mol Sci ; 22(19)2021 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-34638782

RESUMO

Walnut anthracnose caused by Colletotrichum gloeosporioides is a deleterious disease that severely affects the production of walnut (Juglans regia L.). The aim of this study was to assess the antifungal and growth promotion activities of Bacillus velezensis CE 100 as an alternative to chemical use in walnut production. The crude enzyme from B. velezensis CE 100 exhibited chitinase, protease, and ß-l,3-glucanase activity and degraded the cell wall of C. gloeosporioides, causing the inhibition of spore germination and mycelial growth by 99.3% and 33.6% at 100 µL/mL, respectively. The field application of B. velezensis CE 100 culture broth resulted in a 1.3-fold and 6.9-fold decrease in anthracnose disease severity compared to the conventional and control groups, respectively. Moreover, B. velezensis CE 100 produced indole-3-acetic acid (up to 1.4 µg/mL) and exhibited the potential for ammonium production and phosphate solubilization to enhance the availability of essential nutrients. Thus, field inoculation of B. velezensis CE 100 improved walnut root development, increased nutrient uptake, enhanced chlorophyll content, and consequently improved total biomass by 1.5-fold and 2.0-fold compared to the conventional and control groups, respectively. These results demonstrate that B. velezensis CE 100 is an effective biocontrol agent against anthracnose disease and a potential plant growth-promoting bacteria in walnut tree production.


Assuntos
Antifúngicos , Bacillus/química , Colletotrichum/crescimento & desenvolvimento , Misturas Complexas , Juglans , Doenças das Plantas/microbiologia , Raízes de Plantas , Antifúngicos/química , Antifúngicos/farmacologia , Misturas Complexas/química , Misturas Complexas/farmacologia , Juglans/crescimento & desenvolvimento , Juglans/microbiologia , Controle Biológico de Vetores , Raízes de Plantas/crescimento & desenvolvimento , Raízes de Plantas/microbiologia
5.
Molecules ; 26(18)2021 Sep 21.
Artigo em Inglês | MEDLINE | ID: mdl-34577173

RESUMO

In contrast to many human organs, only the human liver can self-regenerate, to some degree. Some marine echinoderms are convenient objects for studying the processes of regenerations of organs and tissues. For example, sea cucumbers Eupentacta fraudatrix can completely restore within several weeks, the internal organs and the whole body after their division into two or three parts. Therefore, these cucumbers are a very convenient model for studying the general mechanisms of regeneration. However, there is no literature data yet on which biomolecules of these cucumbers can stimulate the regeneration of organs and the whole-body processes. Studying the mechanisms of restoration is very important for modern biology and medicine, since it can help researchers to understand which proteins, enzymes, hormones, or possible complexes can play an essential role in regeneration. This work is the first to analyze the possible content of very stable protein complexes in sea cucumbers Eupentacta fraudatrix. It has been shown that their organisms contain a very stable multiprotein complex of about 2000 kDa. This complex contains 15 proteins with molecular masses (MMs) >10 kDa and 21 small proteins and peptides with MMs 2.0-8.6 kDa. It is effectively destroyed only in the presence of 3.0 M MgCl2 and, to a lesser extent, 3.0 M NaCl, while the best dissociation occurs in the presence of 8.0 M urea + 0.1 M EDTA. Our data indicate that forming a very stable proteins complex occurs due to the combination of bridges formed by metal ions, electrostatic contacts, and hydrogen bonds.


Assuntos
Misturas Complexas/química , Proteínas/química , Pepinos-do-Mar/química , Animais , Ligação de Hidrogênio , Metais/química , Peso Molecular , Ligação Proteica , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Eletricidade Estática , Engenharia Tecidual
6.
Sensors (Basel) ; 21(18)2021 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-34577367

RESUMO

High-field asymmetric ion mobility spectrometry (FAIMS) spectra of single chemicals are easy to interpret but identifying specific chemicals within complex mixtures is difficult. This paper demonstrates that the FAIMS system can detect specific chemicals in complex mixtures. A homemade FAIMS system is used to analyze pure ethanol, ethyl acetate, acetone, 4-methyl-2-pentanone, butanone, and their mixtures in order to create datasets. An EfficientNetV2 discriminant model was constructed, and a blind test set was used to verify whether the deep-learning model is capable of the required task. The results show that the pre-trained EfficientNetV2 model completed convergence at a learning rate of 0.1 as well as 200 iterations. Specific substances in complex mixtures can be effectively identified using the trained model and the homemade FAIMS system. Accuracies of 100%, 96.7%, and 86.7% are obtained for ethanol, ethyl acetate, and acetone in the blind test set, which are much higher than conventional methods. The deep learning network provides higher accuracy than traditional FAIMS spectral analysis methods. This simplifies the FAIMS spectral analysis process and contributes to further development of FAIMS systems.


Assuntos
Aprendizado Profundo , Espectrometria de Mobilidade Iônica , Misturas Complexas
7.
Analyst ; 146(19): 5942-5950, 2021 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-34570841

RESUMO

The study of complex mixtures is very important for exploring the evolution of natural phenomena, but the complexity of the mixtures greatly increases the difficulty of material information extraction. Image perception-based machine-learning techniques have the ability to cope with this problem in a data-driven way. Herein, we report a 2D-spectral imaging method to collect matter information from mixture components, and the obtained feature images can be easily provided to deep convolutional neural networks (CNNs) for establishing a spectral network. The results demonstrated that a single CNN trained end-to-end from the proposed images can directly accomplish synchronous measurement of multi-component samples using only raw pixels as inputs. Our strategy has some innate advantages, such as fast data acquisition, low cost, and simple chemical treatment, suggesting that it can be extensively applied in many fields, including environmental science, biology, medicine, and chemistry.


Assuntos
Aprendizado de Máquina , Redes Neurais de Computação , Misturas Complexas , Diagnóstico por Imagem , Processamento de Imagem Assistida por Computador
8.
Int J Mol Sci ; 22(18)2021 Sep 11.
Artigo em Inglês | MEDLINE | ID: mdl-34576003

RESUMO

Noncommunicable diseases (NCD) and age-associated diseases (AAD) are some of the gravest health concerns worldwide, accounting for up to 70% of total deaths globally. NCD and AAD, such as diabetes, obesity, cardiovascular disease, and cancer, are associated with low-grade chronic inflammation and poor dietary habits. Modulation of the inflammatory status through dietary components is a very appellative approach to fight these diseases and is supported by increasing evidence of natural and dietary components with strong anti-inflammatory activities. The consumption of bioactive lipids has a positive impact on preventing chronic inflammation and consequently NCD and AAD. Thus, new sources of bioactive lipids have been sought out. Microalgae are rich sources of bioactive lipids such as omega-6 and -3 polyunsaturated fatty acids (PUFA) and polar lipids with associated anti-inflammatory activity. PUFAs are enzymatically and non-enzymatically catalyzed to oxylipins and have a significant role in anti and pro-resolving inflammatory responses. Therefore, a large and rapidly growing body of research has been conducted in vivo and in vitro, investigating the potential anti-inflammatory activities of microalgae lipids. This review sought to summarize and critically analyze recent evidence of the anti-inflammatory potential of microalgae lipids and their possible use to prevent or mitigate chronic inflammation.


Assuntos
Envelhecimento/efeitos dos fármacos , Misturas Complexas/uso terapêutico , Ácidos Graxos Ômega-3/uso terapêutico , Ácidos Graxos Ômega-6/uso terapêutico , Microalgas/química , Doenças não Transmissíveis/tratamento farmacológico , Misturas Complexas/química , Ácidos Graxos Ômega-3/química , Ácidos Graxos Ômega-6/química , Humanos , Inflamação/tratamento farmacológico
9.
Water Environ Res ; 93(11): 2769-2779, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34477261

RESUMO

In order to transform the dredged sediment (DS) into an efficient water-absorbing ceramsite (EWAC), the coal fly ash (CFA) and expansion agent were used to blend, expand, and sinter with the DS in the Dian Lake-China. A new type of high EWAC was prepared with the absorption ratio of 66.71%, which was much higher than similar products. The heavy metals leaching (HML) of EWAC showed that the concentration of As was 0.90 mg/L and the Hg, Pb, Cd, and Cr were too low to be detected. The characterization analysis showed that the EWAC cross section contained a lot of hydroxyl, ether, and P-Cl hydrophilic group by Fourier transform infrared (FT-IR), scanning electron microscope (SEM), and Brunauer-Emmett-Teller (BET) specific surface area (SSA) test method. The above groups and structures could greatly improve the water absorption (WA) performance of the EWAC. What's more, the SSA of the EWAC could reach 4.468 m2 /g. The results of Comsol Multiphysics indicated that the SSA and average pore size (APS) of the EWACs were 10 and 6 times higher than the commercial ceramsites, respectively. The research provided the utilization of the DS with technical and theoretical basis for the construction of sponge city. PRACTITIONER POINTS: The article was focus on the utilization of dredged sediment (DS) and coal fly ash (CFA) for the basic material preparation technology and its toxicity test as the sponge city. First, the raw materials were the DS in Dian Lake (Kunming, Yunnan, China) and CFA (thermal power plants), which were all belonged to the hazardous solid waste and was made to the efficient water-absorbing ceramsite (EWAC). Second, the water absorption (WA) performance of the EWAC was improved greatly whose absorption ratio was much higher than similar products reached 66.71%. The specific surface area (SSA) and average pore size (APS) of the EWACs were 10 and 6 times higher than the commercial ceramsites (CCs), respectively. Finally, the heavy metals leaching (HML) of As was 0.90 mg/L, and the HML of Hg, Pb, Cd, and Cr was all lower than 0.05 mg/L, which could not only not cause secondary pollution but provide the new ideas for the resource utilization of large amount of DS. So, we thought this article was suitable for the journal.


Assuntos
Cinza de Carvão , Metais Pesados , Carbono , China , Carvão Mineral , Misturas Complexas , Incineração , Lagos , Metais Pesados/análise , Material Particulado , Resíduos Sólidos/análise , Espectroscopia de Infravermelho com Transformada de Fourier , Água
10.
Sci Rep ; 11(1): 17320, 2021 08 27.
Artigo em Inglês | MEDLINE | ID: mdl-34453091

RESUMO

Impact of ultrasound-assisted process (UAP) on yield, functional properties, antioxidant properties and molecular characteristics of protein extracted from Bombay locusts (BL) (Patanga succinta L.) was studied. Different conditions of UAP were implemented for different amplitudes (40-60%) and times (10-30 min) during aqueous extraction. Notably, UAP could enhance yield and protein recovery, compared with those from typical process (TP) (continuously stirred at 100 rpm at room temperature for 1 h). UAP conditions used governed the change of surface hydrophobicity and free α-amino content of BL. UAP could improve solubility of BL, especially at pH levels higher than 2. UAP had no significant (p > 0.05) detrimental effects on foaming capacity and stability of BL. Nevertheless, UAP, particularly at 50-60% amplitudes, affected the emulsion activity and stability of BL. UAP provided BL with high radical scavenging activities and good electron donating ability, especially that from 60% amplitude for 20 min (UAP-60/20). UAP-60/20 showed the impact on change of isoelectric point and molecular characteristic monitored by Fourier transform infrared (FTIR) of BL, compared to those from TP. In addition, BL was also an excellent source of both essential and nonessential amino acids. Therefore, UAP potentially enhanced BL extraction efficiency, resulting the BL with good functional and antioxidative properties.


Assuntos
Antioxidantes/química , Misturas Complexas/química , Gafanhotos/química , Proteínas/química , Aminoácidos/química , Animais , Antioxidantes/farmacologia , Fracionamento Químico , Misturas Complexas/farmacologia , Sequestradores de Radicais Livres/química , Interações Hidrofóbicas e Hidrofílicas , Proteínas/farmacologia , Solubilidade , Temperatura , Ondas Ultrassônicas , Água
11.
Molecules ; 26(15)2021 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-34361776

RESUMO

In this study, we examined aqueous extracts of the edible mushrooms Pleurotus ostreatus (oyster mushroom) and Lentinula edodes (shiitake mushroom). Proteome analysis was conducted using LC-Triple TOF-MS and showed the expression of 753 proteins by Pleurotus ostreatus, and 432 proteins by Lentinula edodes. Bioactive peptides: Rab GDP dissociation inhibitor, superoxide dismutase, thioredoxin reductase, serine proteinase and lectin, were identified in both mushrooms. The extracts also included promising bioactive compounds including phenolics, flavonoids, vitamins and amino acids. The extracts showed promising antiviral activities, with a selectivity index (SI) of 4.5 for Pleurotus ostreatus against adenovirus (Ad7), and a slight activity for Lentinula edodes against herpes simplex-II (HSV-2). The extracts were not cytotoxic to normal human peripheral blood mononuclear cells (PBMCs). On the contrary, they showed moderate cytotoxicity against various cancer cell lines. Additionally, antioxidant activity was assessed using DPPH radical scavenging, ABTS radical cation scavenging and ORAC assays. The two extracts showed potential antioxidant activities, with the maximum activity seen for Pleurotus ostreatus (IC50 µg/mL) = 39.46 ± 1.27 for DPPH; 11.22 ± 1.81 for ABTS; and 21.40 ± 2.20 for ORAC assays. This study encourages the use of these mushrooms in medicine in the light of their low cytotoxicity on normal PBMCs vis à vis their antiviral, antitumor and antioxidant capabilities.


Assuntos
Antineoplásicos/química , Antioxidantes/química , Antivirais/química , Proteínas Fúngicas/química , Pleurotus/química , Proteoma/química , Cogumelos Shiitake/química , Aminoácidos/química , Aminoácidos/isolamento & purificação , Antineoplásicos/isolamento & purificação , Antineoplásicos/farmacologia , Antioxidantes/isolamento & purificação , Antioxidantes/farmacologia , Antivirais/isolamento & purificação , Antivirais/farmacologia , Benzotiazóis/antagonistas & inibidores , Compostos de Bifenilo/antagonistas & inibidores , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Misturas Complexas/química , Flavonoides/química , Flavonoides/isolamento & purificação , Proteínas Fúngicas/classificação , Proteínas Fúngicas/isolamento & purificação , Humanos , Lectinas/química , Lectinas/isolamento & purificação , Leucócitos Mononucleares/citologia , Leucócitos Mononucleares/efeitos dos fármacos , Especificidade de Órgãos , Fenóis/química , Fenóis/isolamento & purificação , Picratos/antagonistas & inibidores , Pleurotus/metabolismo , Cultura Primária de Células , Proteoma/classificação , Proteoma/isolamento & purificação , Serina Proteases/química , Serina Proteases/isolamento & purificação , Cogumelos Shiitake/metabolismo , Ácidos Sulfônicos/antagonistas & inibidores , Superóxido Dismutase/química , Superóxido Dismutase/isolamento & purificação , Tiorredoxina Dissulfeto Redutase/química , Tiorredoxina Dissulfeto Redutase/isolamento & purificação , Vitaminas/química , Vitaminas/isolamento & purificação , Água/química
12.
Molecules ; 26(15)2021 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-34361783

RESUMO

Amber-the fossilized resin of trees-is rich in terpenoids and rosin acids. The physiological effects, such as antipyretic, sedative, and anti-inflammatory, were used in traditional medicine. This study aims to clarify the physiological effects of amber extract on lipid metabolism in mouse 3T3-L1 cells. Mature adipocytes are used to evaluate the effect of amber extract on lipolysis by measuring the triglyceride content, glucose uptake, glycerol release, and lipolysis-related gene expression. Our results show that the amount of triacylglycerol, which is stored in lipid droplets in mature adipocytes, decreases following 96 h of treatment with different concentrations of amber extract. Amber extract treatment also decreases glucose uptake and increases the release of glycerol from the cells. Moreover, amber extract increases the expression of lipolysis-related genes encoding perilipin and hormone-sensitive lipase (HSL) and promotes the activity of HSL (by increasing HSL phosphorylation). Amber extract treatment also regulates the expression of other adipocytokines in mature adipocytes, such as adiponectin and leptin. Overall, our results indicate that amber extract increases the expression of lipolysis-related genes to induce lipolysis in 3T3-L1 cells, highlighting its potential for treating various obesity-related diseases.


Assuntos
Adipócitos/efeitos dos fármacos , Âmbar/farmacologia , Misturas Complexas/farmacologia , Regulação da Expressão Gênica/efeitos dos fármacos , Hipolipemiantes/farmacologia , Lipólise/efeitos dos fármacos , Células 3T3-L1 , Adipócitos/citologia , Adipócitos/metabolismo , Adiponectina/genética , Adiponectina/metabolismo , Âmbar/química , Animais , Diferenciação Celular , Misturas Complexas/química , Etanol/química , Glucose/metabolismo , Glicerol/metabolismo , Hipolipemiantes/química , Leptina/genética , Leptina/metabolismo , Gotículas Lipídicas/química , Gotículas Lipídicas/efeitos dos fármacos , Gotículas Lipídicas/metabolismo , Camundongos , Perilipina-1/genética , Perilipina-1/metabolismo , Fosforilação/efeitos dos fármacos , Esterol Esterase/genética , Esterol Esterase/metabolismo , Triglicerídeos/metabolismo
13.
Environ Toxicol Pharmacol ; 87: 103722, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34391907

RESUMO

We assessed metal/metalloid pollutants (through multi-indices) in seawater, sediments, tissues and shells of gastropods using various indices such as contamination degree (modified/unmodified; Cd/mCd; 1875/187.5). From sediment indices; e.g. the potential ecological risk index/enrichment factor (Eri/EF; 3396.8/105.5) indicated the area to be highly contaminated with metal/metalloid pollutants. Indeed, bioaccumulation with these materials was gastropod size dependent. Antimicrobial and percentage activity indices (AMI/PAI) for; T. telescopium was (AMI/PAI; 1.59/159), N. albicilla (1.14/114) and L. coronata (0.95/95) against E. coli. Similarly T. telescopium (1.33/133), N. albicilla (1.19/119) and L. coronata (1.14/114) have AMI/PAI against A. terreus. The total activity index (TAI), for T. telescopium was the highest, while L. coronata has lowest for all pathogens. This study indicates, T. telescopium, N. albicilla and L. coronata, surviving under metal/metalloid stress exhibited altered natural defense to pathogens which was related to the degree of toxin bioaccumulation.


Assuntos
Arsênio/metabolismo , Gastrópodes/metabolismo , Metais/metabolismo , Poluentes Químicos da Água/metabolismo , Animais , Antibacterianos/farmacologia , Antifúngicos/farmacologia , Arsênio/análise , Bactérias/efeitos dos fármacos , Bactérias/crescimento & desenvolvimento , Bioacumulação , Tamanho Corporal , Misturas Complexas/farmacologia , Monitoramento Ambiental , Fungos/efeitos dos fármacos , Fungos/crescimento & desenvolvimento , Gastrópodes/química , Sedimentos Geológicos/análise , Metais/análise , Poluentes Químicos da Água/análise
14.
J Enzyme Inhib Med Chem ; 36(1): 1829-1838, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34353202

RESUMO

As the largest organ in human body, skin acts as a physicochemical barrier, offering protection against harmful environmental stressors, such as chemicals, pathogens, temperature and radiation. Nonetheless, skins prominence goes further, with a significant psychosocial role in an increasingly ageing population. Prompted by consumers' concern regarding skincare, cosmetic industry has been developing new formulas capable of lessening the most visible signs of ageing, including reduction in skin density and elasticity, wrinkling and hyperpigmentation. Allied to skincare is the rising importance set on natural products, sustainably obtained from less environmental impacting methods. Cyanobacteria and microalgae are adding importance in this field, given their ability to biosynthesize secondary metabolites with anti-ageing potential. In this review, we present an overview on the potential of cyanobacteria and microalgae compounds to overcome skin-ageing, essentially by exploring their effects on the metalloproteinases collagenase, elastase, gelatinase and hyaluronidase, and in other enzymes involved in the pigmentation process.


Assuntos
Antioxidantes/química , Misturas Complexas/química , Cianobactérias/química , Matriz Extracelular/metabolismo , Hiperpigmentação/tratamento farmacológico , Microalgas/química , Envelhecimento da Pele/efeitos dos fármacos , Antioxidantes/farmacologia , Produtos Biológicos/química , Colagenases/metabolismo , Misturas Complexas/farmacologia , Gelatinases/metabolismo , Humanos , Hialuronoglucosaminidase/metabolismo , Metaloproteases/metabolismo , Elastase Pancreática/metabolismo , Pele
15.
Fish Shellfish Immunol ; 117: 291-298, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34419600

RESUMO

This study investigated the immunomodulatory effects of Sargassum polycystum extract administration in rainbow trout (Oncorhynchus mykiss). S. polycystum methanolic extract was administered orally using feeding needles to individual rainbow trout at the dose of 0 (control), 1 (S1), 3 (S3) and 5 (S5) mg/100 µl/per fish twice a day for 7 days. On 1st, 5th, 3rd and 7th day, blood and tissues were collected from the fish and changes in humoral immune responses and immune-related gene expressions were determined. The result of oxidative radical production showed no difference during early stage of the experiment and was lately decreased (P < 0.05). Lysozyme activity increased on 3rd and 7th day of the study in S5 fish group and on 5th day in S3 group compared to control (P < 0.05). Myeloperoxidase activity had an increased level on the 1st and 3rd day in S1, S5 and S5 fish groups, respectively. IL-1ß gene was significantly up-regulated in kidney and intestine in all experimental groups (except on the 1st day, in the intestine of S5 fish group) compared to control (P < 0.05). IL-8 gene expression was elevated on 1st and 3rd day in kidney of all experimental fish groups. IL-6 transcript enhanced in a dose-dependent manner on 3rd and 7th day. IL-10 and IL-12 genes were also up-regulated. Survival in all treated fish groups challenged with Aeromonas hydrophila was significantly increased compared to that of control. The highest survival rate was recorded in S5 fish group (83.65%) followed by S3 fish group (82.62%). Our results suggest that S. polycystum aqueous methanolic extract is an effective immunostimulant and provide protection against A. hydrophila infection in rainbow trout at a dose of 3-10 mg/20 g body weight/day.


Assuntos
Adjuvantes Imunológicos/farmacologia , Aeromonas hydrophila , Misturas Complexas/farmacologia , Doenças dos Peixes/imunologia , Infecções por Bactérias Gram-Negativas/imunologia , Oncorhynchus mykiss/imunologia , Sargassum , Administração Oral , Animais , Citocinas/genética , Citocinas/imunologia , Doenças dos Peixes/mortalidade , Infecções por Bactérias Gram-Negativas/mortalidade , Infecções por Bactérias Gram-Negativas/veterinária , Muramidase/imunologia , Oncorhynchus mykiss/genética , Oncorhynchus mykiss/microbiologia , Peroxidase/imunologia
16.
Biomolecules ; 11(8)2021 08 02.
Artigo em Inglês | MEDLINE | ID: mdl-34439804

RESUMO

The bioprospecting of marine and brackish water systems has increased during the last decades. In this respect, microalgae, including cyanobacteria, and their metabolites are one of the most widely explored resources. Most of the bioactive compounds are isolated from ex situ cultures of microorganisms; however, analysis of field samples could also supply valuable information about the metabolic and biotechnological potential of microalgae communities. In this work, the activity of phytoplankton samples from the Curonian Lagoon was studied. The samples were active against antibiotic resistant clinical and environmental bacterial strains as well as against serine proteases and T47D human breast adenocarcinoma cells. No significant effect was found on Daphnia magna. In addition, using LC-MS/MS, we documented the diversity of metabolites present in field samples. A list of 117 detected cyanopeptides was presented. Cyanopeptolins constituted the largest class of cyanopeptides. As complex bloom samples were analyzed, no link between the observed activity and a specific sample component can be established. However, the results of the study showed a biotechnological potential of natural products from the Curonian Lagoon.


Assuntos
Antibacterianos/farmacologia , Antineoplásicos/farmacologia , Produtos Biológicos/farmacologia , Cianobactérias/química , Microalgas/química , Fitoplâncton/química , Animais , Antibacterianos/química , Antineoplásicos/química , Oceano Atlântico , Países Bálticos , Baías/microbiologia , Produtos Biológicos/química , Linhagem Celular Tumoral , Misturas Complexas/química , Misturas Complexas/farmacologia , Cianobactérias/metabolismo , Daphnia/efeitos dos fármacos , Daphnia/fisiologia , Depsipeptídeos/química , Depsipeptídeos/farmacologia , Eutrofização , Água Doce/microbiologia , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Negativas/crescimento & desenvolvimento , Bactérias Gram-Positivas/efeitos dos fármacos , Bactérias Gram-Positivas/crescimento & desenvolvimento , Humanos , Microalgas/metabolismo , Testes de Sensibilidade Microbiana , Peptídeos Cíclicos/química , Peptídeos Cíclicos/farmacologia , Fitoplâncton/metabolismo , Águas Salinas/química , Serina Proteases/metabolismo
17.
Cells ; 10(7)2021 07 20.
Artigo em Inglês | MEDLINE | ID: mdl-34360010

RESUMO

BACKGROUND: Until now, the cost of allergy treatment in the insured public health care system and the non-insured self-financing private health care system in Indonesia has not been well documented and published, as well as the cost of allergy treatment with subcutaneous immunotherapy. OBJECTIVE: To evaluate the clinical and cost benefits of allergic rhinitis treatment in children with subcutaneous immunotherapy in a non-insured self-financing private health care system. METHODS: A retrospective cohort study conducted from 2015 until 2020 that compared the clinical improvement and health care costs over 18 months in newly diagnosed AR children who received SCIT versus matched AR control subjects who did not receive SCIT, with each group consisting of 1098 subjects. RESULTS: A decrease in sp-HDM-IgE level (kU/mL) from 20.5 + 8.75 kU/mL to 12.1 + 3.07 kU/mL was observed in the SCIT group. To reduce the symptom score of allergic rhinitis by 1.0 with SCIT, it costs IDR 21,753,062.7 per child, and for non-SCIT, it costs IDR 104,147,878.0 per child. Meanwhile, to reduce the medication score (MS) by 1.0 with SCIT, it costs IDR 17,024,138.8, while with non-SCIT, it costs IDR 104,147,878.0. Meanwhile, to lower combination symptoms and medication score (CSMS) by 1.0, with SCIT, it costs IDR 9,550,126.6, while with non-SCIT, it costs IDR 52,073,938.9. CONCLUSIONS: In conclusion, this first Indonesia-based study demonstrates substantial health care cost savings associated with SCIT for children with AR in an uninsured private health care system and provides strong evidence for the clinical benefits and cost-savings benefits of AR treatment in children.


Assuntos
Análise Custo-Benefício , Dermatophagoides pteronyssinus/imunologia , Dessensibilização Imunológica/economia , Rinite Alérgica/economia , Rinite Alérgica/terapia , Adolescente , Alérgenos/administração & dosagem , Alérgenos/química , Alérgenos/imunologia , Animais , Criança , Pré-Escolar , Misturas Complexas/administração & dosagem , Misturas Complexas/isolamento & purificação , Dermatophagoides pteronyssinus/química , Dessensibilização Imunológica/métodos , Feminino , Humanos , Imunoglobulina E/sangue , Indonésia , Lactente , Recém-Nascido , Injeções Subcutâneas , Masculino , Prática Privada/economia , Estudos Retrospectivos , Rinite Alérgica/imunologia , Rinite Alérgica/patologia
18.
Sci Rep ; 11(1): 16711, 2021 08 18.
Artigo em Inglês | MEDLINE | ID: mdl-34408181

RESUMO

The isolated protein-polysaccharide fraction (AAF) from the coelomic fluid of Dendrobaena veneta earthworm shows effective activity against Candida albicans yeast. Fungal cells of the clinical strain after incubation with the active fraction were characterized by disturbed cell division and different morphological forms due to the inability to separate the cells from each other. Staining of the cells with acridine orange revealed a change in the pH of the AAF-treated cells. It was observed that, after the AAF treatment, the mitochondrial DNA migrated towards the nuclear DNA, whereupon both merged into a single nuclear structure, which preceded the apoptotic process. Cells with a large nucleus were imaged with the scanning electron cryomicroscopy (Cryo-SEM) technique, while enlarged mitochondria and the degeneration of cell structures were shown by transmission electron microscopy (TEM). The loss of the correct cell shape and cell wall integrity was visualized by both the TEM and SEM techniques. Mass spectrometry and relative quantitative SWATH MS analysis were used to determine the reaction of the C. albicans proteome to the components of the AAF fraction. AAF was observed to influence the expression of mitochondrial and oxidative stress proteins. The oxidative stress in C. albicans cells caused by the action of AAF was demonstrated by fluorescence microscopy, proteomic methods, and XPS spectroscopy. The secondary structure of AAF proteins was characterized by Raman spectroscopy. Analysis of the elemental composition of AAF confirmed the homogeneity of the preparation. The observed action of AAF, which targets not only the cell wall but also the mitochondria, makes the preparation a potential antifungal drug killing the cells of the C. albicans pathogen through apoptosis.


Assuntos
Antifúngicos , Candida albicans , Misturas Complexas , Proteínas Fúngicas/metabolismo , Oligoquetos/química , Polissacarídeos , Proteômica , Animais , Antifúngicos/química , Antifúngicos/farmacologia , Candida albicans/metabolismo , Candida albicans/ultraestrutura , Misturas Complexas/química , Misturas Complexas/farmacologia , Mitocôndrias/metabolismo , Mitocôndrias/ultraestrutura , Polissacarídeos/química , Polissacarídeos/farmacologia
19.
Molecules ; 26(16)2021 Aug 13.
Artigo em Inglês | MEDLINE | ID: mdl-34443495

RESUMO

Phytochemical investigation and chromatographic separation of extracts from the actinobacteria strain Saccharomonospora piscinae that was isolated from dried fishpond sediment of Kouhu township, in the south of Taiwan, led to the isolation of three new compounds, saccharpiscinols A-C (1-3, respectively), and three new natural products, namely (2S)-5,7,3',4'-tetrahydroxy-6,8-dimethylflavanone (4), methyl-4-hydroxy-2-methoxy-6-methylbenzoate (5), and (±)-7-acetyl-4,8-dihydroxy-6-methyl-1-tetralone (6). Compounds 4-6 were reported before as synthesized products, herein, they are reported from nature for the first time. The structures of the new compounds were unambiguously elucidated on the basis of extensive spectroscopic data analysis (1D- and 2D-NMR, MS, and UV) and comparison with literature data. The effect of some isolates on the inhibition of NO production in lipopolysaccharide-activated RAW 264.7 murine macrophages was evaluated. Saccharpiscinol A showed inhibitory activities against LPS-induced NO production.


Assuntos
Actinobacteria/química , Anti-Inflamatórios/farmacologia , Flavonoides/farmacologia , Animais , Anti-Inflamatórios/química , Sobrevivência Celular/efeitos dos fármacos , Cromatografia Líquida de Alta Pressão , Misturas Complexas , Flavonoides/química , Lipopolissacarídeos/farmacologia , Macrófagos/efeitos dos fármacos , Macrófagos/metabolismo , Camundongos , Óxido Nítrico/biossíntese , Células RAW 264.7
20.
Molecules ; 26(16)2021 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-34443386

RESUMO

Lectins facilitate cell-cell contact and are critical in many cellular processes. Studying lectins may help us understand the mechanisms underlying tissue regeneration. We investigated the localization of an R-type lectin in a marine annelid (Perinereis sp.) with remarkable tissue regeneration abilities. Perinereis nuntia lectin (PnL), a galactose-binding lectin with repeating Gln-X-Trp motifs, is derived from the ricin B-chain. An antiserum was raised against PnL to specifically detect a 32-kDa lectin in the crude extracts from homogenized lugworms. The antiserum detected PnL in the epidermis, setae, oblique muscle, acicula, nerve cord, and nephridium of the annelid. Some of these tissues and organs also produced Galactose (Gal) or N-acetylgalactosamine (GalNAc), which was detected by fluorescent-labeled plant lectin. These results indicated that the PnL was produced in the tissues originating from the endoderm, mesoderm, and ectoderm. Besides, the localizing pattern of PnL partially merged with the binding pattern of a fluorescent-labeled mushroom lectin that binds to Gal and GalNAc. It suggested that PnL co-localized with galactose-containing glycans in Annelid tissue; this might be the reason PnL needed to be extracted with haptenic sugar, such as d-galactose, in the buffer. Furthermore, we found that a fluorescein isothiocyanate-labeled Gal/GalNAc-binding mushroom lectin binding pattern in the annelid tissue overlapped with the localizing pattern of PnL. These findings suggest that lectin functions by interacting with Gal-containing glycoconjugates in the tissues.


Assuntos
Anelídeos/metabolismo , Organismos Aquáticos/metabolismo , Lectinas/metabolismo , Animais , Misturas Complexas , Ligantes , Polissacarídeos/metabolismo
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