Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 280
Filtrar
1.
J Environ Sci (China) ; 115: 411-421, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-34969469

RESUMO

Iron-oxidizing strain (FeOB) and iron modified biochars have been shown arsenic (As) remediation ability in the environment. However, due to the complicated soil environment, few field experiment has been conducted. The study was conducted to investigate the potential of iron modified biochar (BC-FeOS) and biomineralization by a new found FeOB to remediate As-contaminated paddy field. Compared with the control, the As contents of GB (BC-FeOS), GF (FeOB), GFN (FeOB and nitrogen fertilizer), GBF (BC-FeOS and FeOB) and GBFN (BC-FeOS, FeOB and nitrogen fertilizer) treatments in pore water decreased by 36.53%-80.03% and the microbial richness of iron-oxidizing bacteria in these treatments increased in soils at the rice maturation stage. The concentrations of available As of GB, GF, GFN, GBF and GBFN at the tillering stage were significantly decreased by 10.78%-55.48%. The concentrations of nonspecifically absorbed and specifically absorbed As fractions of GB, GF, GFN, GBF and GBFN in soils were decreased and the amorphous and poorly crystalline hydrated Fe and Al oxide-bound fraction was increased. Moreover, the As contents of GB, GF, GFN, GBF and GBFN in rice grains were significantly decreased (*P < 0.05) and the total As contents of GFN, GBF and GBFN were lower than the standard limit of the National Standard for Food Safety (GB 2762-2017). Compared with the other treatments, GBFN showed the greatest potential for the effective remediation of As-contaminated paddy fields.


Assuntos
Arsênio , Ochrobactrum , Oryza , Poluentes do Solo , Arsênio/análise , Carvão Vegetal , Ferro/análise , Oxirredução , Solo , Poluentes do Solo/análise
2.
BMC Infect Dis ; 21(1): 1252, 2021 Dec 14.
Artigo em Inglês | MEDLINE | ID: mdl-34906070

RESUMO

BACKGROUND: Ochrobactrum spp. are non-fermenting, Gram-negative bacilli that are regarded as emerging human pathogens of low virulence that can cause infections. The first identified case of Ochrobactrum intermedium was reported in 1998 in a liver transplantation patient with liver abcess. There are no reports of infections in pediatric patients. Here, we report the first case of O. intermedium bacteremia in a pediatric patient. CASE PRESENTATION: A two and a half years old male was admitted with fever, chills and nausea. He had been diagnosed as pineoblastoma and underwent surgical resection and chemotherapy. O. intermedium was isolated from his blood cultures and identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), however, the Vitek II automated system failed to identify the organism. Then the pathogen was confirmed by 16S rDNA sequencing and average nucleotide identity result (ANI) confirmed the precise identification of O. intermedium at genomic level. In addition, the patient recovered well after antibiotic combined therapy. CONCLUSIONS: This, to our knowledge, is the first case of O. intermedium bacteremia in a pediatric patient with malignant tumor. Traditional biochemical identification methods such as API 20NE or VITEK2 system cannot differentiate O. anthropi and O. intermedium. MALDI-TOF may be a promising tool for rapid identification of microorganisms such as O. intermedium.


Assuntos
Bacteriemia , Infecções por Bactérias Gram-Negativas , Neoplasias , Ochrobactrum , Bacteriemia/diagnóstico , Pré-Escolar , Infecções por Bactérias Gram-Negativas/diagnóstico , Infecções por Bactérias Gram-Negativas/tratamento farmacológico , Humanos , Masculino , Ochrobactrum/genética
3.
Int J Chron Obstruct Pulmon Dis ; 16: 2925-2937, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34737557

RESUMO

Background: Particulate matter-associated microbes in the workplace are a burning issue in occupational toxicology. Studies have reported on respiratory infections among tannery cohorts. This study uniquely presents measurements of airborne bacterial concentrations associated with varied particulate-matter sizes, their exposure, and consequent severity in occupational respiratory problems, all for different microenvironments within leather tanneries. Methods: Analyses included molecular identification of isolates, computation of mass median aerodynamic diameter of aerosols, tannery process-exposure dose (TPED) to bacterial aerosols, and spirometry and symptom assessment of impaired pulmonary function. Results: The highest bacterial concentrations were for rawhide treatment and finishing units, showing 3.6×103 and 3.7×103 CFU/m3, respectively. Identified bacterial species included Ochrobactrum pseudogrignonense, Neisseria bacilliformis, Enterobacter cloacae, Alcaligenes faecalis, Klebsiella pneumoniae, and Corynebacterium spp. Maximum and minimum values of mass median aerodynamic diameter were 8.3 µm and 0.65 µm for buffing and snuffing and production units, respectively. The highest TPED was 1,516.9 CFU/kg for finishing units. Respiratory symptoms in order of incidence were dyspnea > phlegm > cough > wheezing and tachypnea (equivalent). Bronchodilator measurements of FEV1, FVC, and PEF represent decline in lung function. Of 26 patients identified with COPD, most were working in rawhide treatment. Conclusion: We conclude that exposure-infection synergy is also a cause of pulmonary ailments and COPD development, rather than the better-known exposure-smoking synergy.


Assuntos
Exposição Ocupacional , Doença Pulmonar Obstrutiva Crônica , Aerossóis , Humanos , Neisseria , Exposição Ocupacional/efeitos adversos , Ochrobactrum , Paquistão , Doença Pulmonar Obstrutiva Crônica/diagnóstico , Doença Pulmonar Obstrutiva Crônica/epidemiologia
4.
J Hazard Mater ; 420: 126651, 2021 10 15.
Artigo em Inglês | MEDLINE | ID: mdl-34329075

RESUMO

The present study investigates the individual degrading behavior of bacterial strains isolated from glyphosate-degrading stream biofilms. In this aim, biofilms were subjected to enrichment experiments using glyphosate or its metabolite AMPA (aminomethyl phosphonic acid) as the sole phosphorus source. Five bacterial strains were isolated and taxonomically affiliated to Ensifer sp. CNII15, Acidovorax sp. CNI26, Agrobacterium tumefaciens CNI28, Novosphingobium sp. CNI35 and Ochrobactrum pituitosum CNI52. All strains were capable of completely dissipating glyphosate after 125-400 h and AMPA after 30-120 h, except for Ensifer sp. CNII15 that was not able to dissipate glyphosate but entirely dissipated AMPA after 200 h. AMPA dissipation was overall faster than glyphosate dissipation. The five strains degraded AMPA completely since formaldehyde and/or glycine accumulation was observed. During glyphosate degradation, the strain CNI26 used the C-P lyase degradation pathway since sarcosine was quantitatively produced, and C-P lyase gene expression was enhanced 30× compared to the control treatment. However, strains CNI28, CNI35 and CNI52 accumulated both formaldehyde and glycine after glyphosate transformation suggesting that both C-P lyase and/or glyphosate oxidase degradation pathways took place. Our study shows different and complementary glyphosate degradation pathways for bacteria co-existing in stream biofilms.


Assuntos
Herbicidas , Rios , Bactérias , Biofilmes , Glicina/análogos & derivados , Ochrobactrum
5.
J Basic Microbiol ; 61(8): 757-768, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34101885

RESUMO

The purpose of this study was to isolate lignin-degrading bacteria from buffalo rumen and to explore their interactions further. Using lignin as the carbon source, three bacteria, B-04 (Ochrobactrum pseudintermedium), B-11 (Klebsiella pneumoniae), and B-45 (Bacillus sonorensis), which have shown lignin degradation potential, were successfully isolated and identified from the rumen fluid of buffalo by colony morphology, 16S ribosomal RNA gene sequencing, and biochemical and physiological analyses. The degradation rates of lignin were determined, and the maximum values were 4.86%, 11.1%, and 7.68% for B-04, B-11, and B-45, respectively. The maximum laccase activities were 0.65, 0.93, and 1.15 U/ml, while the maximum lignin peroxidase activities were 5.72, 8.29, and 18.69 U/ml, respectively. Pairwise interaction studies showed inhibitory interaction between B-04 and B-45, inhibitory interaction between B-04 and B-11, and symbiotic interaction between B-11 and B-45. This is the first report on the lignin degradation ability of bacteria isolated from the buffalo's rumen, which provides a new understanding for revealing the mechanism of roughage tolerance of buffalo.


Assuntos
Bactérias/isolamento & purificação , Bactérias/metabolismo , Búfalos/microbiologia , Lignina/metabolismo , Rúmen/microbiologia , Animais , Bacillus/isolamento & purificação , Bacillus/metabolismo , Bactérias/classificação , Bactérias/genética , Klebsiella pneumoniae/isolamento & purificação , Klebsiella pneumoniae/metabolismo , Interações Microbianas , Ochrobactrum/isolamento & purificação , Ochrobactrum/metabolismo , Filogenia , RNA Ribossômico 16S/genética , Alinhamento de Sequência
6.
Microb Cell Fact ; 20(1): 117, 2021 Jun 13.
Artigo em Inglês | MEDLINE | ID: mdl-34120587

RESUMO

BACKGROUND: Biodegradation of antibiotics is a promising method for the large-scale removal of antibiotic residues in the environment. However, the enzyme that is involved in the biodegradation process is the key information to be revealed. RESULTS: In this study, the beta-lactamase from Ochrobactrum tritici that mediates the biodegradation of penicillin V was identified and characterized. When searching the proteins of Ochrobactrum tritici, the ß-lactamase (OtLac) was identified. OtLac consists of 347 amino acids, and predicted isoelectric point is 7.0. It is a class C ß-lactamase according to BLAST analysis. The coding gene of OtLac was amplified from the genomic DNA of Ochrobactrum tritici. The OtLac was overexpressed in E. coli BL21 (DE3) and purified with Ni2+ column affinity chromatography. The biodegradation ability of penicillin V by OtLac was identified in an in vitro study and analyzed by HPLC. The optimal temperature for OtLac is 32 â„ƒ and the optimal pH is 7.0. Steady-state kinetics showed that OtLac was highly active against penicillin V with a Km value of 17.86 µM and a kcat value of 25.28 s-1 respectively. CONCLUSIONS: OtLac demonstrated biodegradation activity towards penicillin V potassium, indicating that OtLac is expected to degrade penicillin V in the future.


Assuntos
Ochrobactrum/enzimologia , Ochrobactrum/genética , Penicilinas/metabolismo , beta-Lactamases/genética , beta-Lactamases/metabolismo , Antibacterianos/metabolismo , Biodegradação Ambiental , Catálise , Clonagem Molecular , DNA Bacteriano , Fermentação , Concentração de Íons de Hidrogênio , Cinética , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/metabolismo , Temperatura
7.
Enzyme Microb Technol ; 148: 109789, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34116752

RESUMO

Ribose-5-phosphate isomerase A (RpiA) is of great importance in biochemistry research, however its application in biotechnology has not been fully explored. In this study the activity of RpiA from Ochrobactrum sp. CSL1 (OsRpiA) towards D-allose was engineered based on sequential and structural analyses. Strategies of alanine scanning, rational design and saturated mutagenesis were employed to create three mutant libraries. A single mutant of K124A showed a 45 % activity improvement towards D-allose. The reaction properties of the mutant were analyzed, and a shift of optimal pH and higher thermal stability at low reaction temperatures were identified. The conversion of D-allose was also improved by 40 % using K124A, and higher activities on major substrates were found in the mutant's substrate scope, implying its application potential in rare sugar preparation. Kinetics analysis revealed that Km of K124A mutant decreased by 12 % and the catalytic efficiency increased by 65 % towards D-allose. Moreover, molecular dynamics simulation illustrated the binding of substrate and K124A was more stable than that of the wild-type. The shorter distance and more relax bond angle between the catalytic residue of K124A and D-allose explained the activity improvement in detail. This study highlights the potential of OsRpiA as a biocatalyst for rare sugar preparation, and provides distinct evidences for its catalytic mechanism.


Assuntos
Aldose-Cetose Isomerases , Ochrobactrum , Aldose-Cetose Isomerases/genética , Aldose-Cetose Isomerases/metabolismo , Isomerismo , Ochrobactrum/metabolismo , Açúcares
8.
Chemosphere ; 282: 131064, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34118631

RESUMO

Sulfate-reducing bacteria (SRB) are key players in many passive and active systems dedicated to the treatment of hydrometallurgical leachates. One of the main factors reducing the efficiency and activity of SRB is the low pH and poor nutrients in leachates. We propose an innovative solution utilizing biogenic ammonia (B-NH3), produced by urea degrading bacteria, as a pretreatment agent for increasing the pH of the leachate and spontaneously stimulating SRB activity via bacterial secondary metabolites. The selected strain, Ochrobactrum sp. POC9, generated 984.7 mg/L of ammonia in 24 h and promotes an effective neutralization of B-NH3. The inferred metabolic traits indicated that the Ochrobactrum sp. POC9 can synthesize a group of vitamins B, and the production of various organic metabolites was confirmed by GC-MS analysis. These metabolites comprise alcohols, organic acids, and unsaturated hydrocarbons that may stimulate biological sulfate reduction. With the pretreatment of B-NH3, sulfate removal efficiency reached ~92.3% after 14 days of incubation, whereas SRB cell count and abundance were boosted (~107 cell counts and 88 OTUs of SRB) compared to synthetic ammonia (S-NH3) (~103 cell counts and 40 OTUs of SRB). The dominant SRB is Desulfovibrio in both S-NH3 and B-NH3 pretreated leachate, however, it belonged to two different clades. By reconstructing the ecological network, we found that B-NH3 not only directly increases SRB performance but also promotes other strains with positive correlations with SRB.


Assuntos
Desulfovibrio , Microbiota , Ochrobactrum , Bactérias , Sulfatos , Ureia
9.
Lett Appl Microbiol ; 73(3): 326-335, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34060111

RESUMO

An organophosphorus pesticide malathion biodegradation was investigated by using the bacteria Ochrobactrum sp. M1D isolated from a soil sample of peach orchards in Palampur, District Kangra, Himachal Pradesh (India). The bacterium was able to utilize malathion as the sole source of carbon and energy. The isolated bacterium was found psychrotolerant and could degrade 100% of 100 mg l-1 malathion in minimal salt medium at 20°C, pH 7·0 within 12 days with no major significant metabolites left at the end of the study. Through GCMS analysis, methyl phosphate, diethyl maleate, and diethyl 2-mercaptosuccinate were detected and identified as the major pathway metabolites. Based on the GCMS profile, three probable degradation pathways were interpreted. The present study is the first report of malathion biodegradation at both the psychrophilic and mesophilic conditions by any psychrotolerant strain and also through multiple degradation pathways. In the future, the strain can be explored to bio-remediate the malathion contaminated soil in the cold climatic region and to utilize the enzymatic systems for advanced biotechnology applications.


Assuntos
Ochrobactrum , Praguicidas , Bactérias , Biodegradação Ambiental , Malation , Redes e Vias Metabólicas , Ochrobactrum/genética , Compostos Organofosforados , Microbiologia do Solo
10.
Int Microbiol ; 24(3): 441-453, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-33987705

RESUMO

Globally, the underlying peril of cumulative toxicity of heavy metals in water bodies contaminated by industrial effluents is a matter of great concern to the environmentalists. Heavy metals like lead, cadmium, and nickel are particularly liable for this. Such toxic water is not only hazardous to human health but also harmful to aquatic animals. Remedial measures are being taken by physico-chemical techniques, but most of them are neither eco-friendly nor cost-effective. Biological means like bioaccumulation of heavy metals by viable bacteria are often tedious. In the present study, biosorption of heavy metals is successfully expedited by surfactant exopolysaccharide (SEPS) of Ochrobactrum pseudintermedium C1 as a simple, safe, and economically sustainable option utilizing an easily available and cost-effective substrate like molasses extract. Its efficacy in bioremediation of toxic heavy metals like cadmium, nickel, and lead have been studied by UV-Vis spectrophotometry and verified by inductively coupled plasma-atomic emission spectroscopy (ICP-AES). FTIR and zeta potential studies have also been carried out to explore this novel biosorption potential. Results are conclusive and promising. Moreover, this particular SEPS alone can remediate all these three toxic heavy metals in water. For futuristic applications, it might be a prospective and cost-effective resource for bioremediation of toxic heavy metals in aqueous environment.


Assuntos
Metais Pesados/metabolismo , Ochrobactrum/metabolismo , Polissacarídeos Bacterianos/metabolismo , Tensoativos/metabolismo , Poluentes Químicos da Água/metabolismo , Biodegradação Ambiental , Cádmio/metabolismo , Análise Custo-Benefício , Chumbo/metabolismo , Microscopia Eletrônica de Varredura , Níquel/metabolismo , Polissacarídeos Bacterianos/ultraestrutura
11.
Environ Sci Pollut Res Int ; 28(35): 48718-48727, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-33913111

RESUMO

Sulfur-containing wastewater is very common as an industrial waste, yet a high-efficiency composite microbial agent for sulfur-containing wastewater treatment is still lacking. In this work, three novel and efficient desulfurizing bacteria were isolated from the sewage treatment tank of Zhejiang Satellite Energy Co., Ltd. They were identified as Brucella melitensis (S1), Ochrobactrum oryzae (S8), and Achromobacter xylosoxidans (S9). These three strains of bacteria were responsible for the oxidative metabolism of sodium sulfide via a similar polythionate pathway, which could be expressed as follows: S2-→S2O32-/S0→SO32-→SO42-. Activated carbon, wheat bran, and diatomite at 1:1:1 ratio are used as carriers to construct a composite microbial agent containing the three bacteria. The desulfurization efficiency of 95% was predicted by response surface methodology under the following optimum conditions: the dosage of the inoculum was 3 g/L, pH 7.86, and temperature of 39 °C. Additionally, the impact resistance was studied in the anaerobic sequencing batch reactor. The removal capacity of microbial agent reached 98%. High-throughput analysis showed that composite microbial agent increased bacterial evenness and diversity, and the relative abundance of Brucellaceae increased from 5.04 to 8.79% in the reactor. In the process of industrial wastewater transformation, the transformation rate of sulfide by composite microbial agent was maintained between 70 and 81%. The composite microbial agent had potential for the treatment of sulfur-containing wastewater.


Assuntos
Microbiota , Esgotos , Anaerobiose , Reatores Biológicos , Biotransformação , Ochrobactrum , Sulfetos , Eliminação de Resíduos Líquidos , Águas Residuárias
12.
Microbiol Res ; 246: 126703, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33482437

RESUMO

Production and release of organic acids and phosphatase enzymes by microbes are important for inorganic and organic phosphorus cycling in soil. The presence of microorganisms with corresponding traits in the plant rhizosphere lead to improved plant P uptake and ultimately growth promotion. We studied the potential of two rhizosphere-competent strains, Pantoea sp. MR1 and Ochrobactrum sp. SSR, for solubilization of different organic and inorganic P sources in vitro. In a pot experiment we further revealed the impact of the two strains on wheat seedling performance in soil amended with either phytate, rock phosphate or K2HPO4 as solely P source. To directly link P-solubilizing activity to the strain-specific genetic potential, we designed novel primers for glucose dehydrogenase (gcd), phosphatase (pho) and phytase (phy) genes, which are related to the organic and inorganic P solubilization potential. Quantitative tracing of these functional genes in the inoculated soils of the conducted pot experiment further allowed to compare strain abundances in the soil in dependency on the present P source. We observed strain- and P source-dependent patterns of the P solubilization in vitro as well as in the pot experiment, whereby P release, particularly from phytate, was linked to the strain abundance. We further revealed that the activity of microbial phosphatases is determined by the interplay between functional gene abundance, available soil P, and substrate availability. Moreover, positive impacts of microbial seed inoculation on wheat root architecture and aboveground growth parameters were observed. Our results suggest that screening for rhizosphere-competent strains with gcd, pho and phy genes may help to identify new microbial taxa that are able to solubilize and mineralize inorganic as well as organic bound P. Subsequently, the targeted use of corresponding strains may improve P availability in agricultural soils and consequently reduce fertilizer application.


Assuntos
Ochrobactrum/genética , Pantoea/genética , Fósforo/metabolismo , Triticum/crescimento & desenvolvimento , 6-Fitase/genética , Proteínas de Bactérias/genética , Glucose 1-Desidrogenase/genética , Ochrobactrum/enzimologia , Pantoea/enzimologia , Fosfatos/metabolismo , Monoéster Fosfórico Hidrolases/genética , Filogenia , Ácido Fítico/metabolismo , Raízes de Plantas/microbiologia , Rizosfera , Plântula/crescimento & desenvolvimento , Plântula/metabolismo , Solo/química , Microbiologia do Solo , Triticum/metabolismo
13.
Ticks Tick Borne Dis ; 12(1): 101588, 2021 01.
Artigo em Inglês | MEDLINE | ID: mdl-33075731

RESUMO

Ticks are common vectors of human and animal diseases. Ochrobactrum spp. belong to the Brucellaceae family and have recently been recognized as emerging human pathogens. The ability of Haemaphysalis longicornis ticks to carry Ochrobactrum spp. remains uncertain. During June and July 2018, 686 ticks were collected from 11 sites in Pingdingshan Henan province in central China. We extracted 169 DNA samples for Brucellaceae 16S rRNA nested PCR and sequenced them in order to identify Ochrobactrum spp. The data sequences were aligned with NCBI BLAST program and phylogenetic tree was constructed using Mega 5.0. Twenty samples were sequenced successfully out of a total forty-one positive for Brucellaceae. Thirteen DNA samples were identical to O. intermedium (99.85 %-100.00 %) and 3 were identical to O. cicer (99.85 %-100.00 %) (15 collected from host and one from vegetation). Four DNA samples (3 collected from host and one from vegetation) had 99.83-100 % B. melitensis identity. This study adds to the growing body of evidence that shows Ochrobactrum spp. are present in H. longicornis. Ochrobactrum spp. and Brucella spp. are phenotypically and genetically closely related pathogens. Our finding highlights the importance of gene sequencing and phylogenetic analysis to differentiate between Ochrobactrum spp. and Brucella spp. in the research and potentially clinical setting. Future work is required to investigate the transmission potential of Ochrobactrum spp. by H. longicornis.


Assuntos
Brucella/isolamento & purificação , Ixodidae/microbiologia , Ochrobactrum/isolamento & purificação , Animais , Ixodidae/crescimento & desenvolvimento , Larva/crescimento & desenvolvimento , Larva/microbiologia , Filogenia , Reação em Cadeia da Polimerase , RNA Bacteriano/análise , RNA Ribossômico 16S/análise
14.
Bioresour Technol ; 320(Pt B): 124330, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33202345

RESUMO

The present study describes the heavy metal bioaccumulation potential of Ochrobactrum intermedium BPS-20 and Ochrobactrum ciceri BPS-26. A total of 27 isolates were retrieved from the soils of industrial areas and these two were selected based on their maximum metal tolerance. They can resist up to 2400 mg/L and 2000 mg/L of Lead and 850 mg/L and 1200 mg/L of Nickel respectively. The atomic absorption spectroscopic analysis showed considerably good bioaccumulation by O. intermedium BPS-20 (85.34% and 74.87%) and O. ciceri BPS-26 (71.20% and 88.48%) for Lead and Nickel respectively. The growth rate studies also demonstrated no inhibitory effects of heavy metals in the medium. Further the SEM analysis showed the presence of extracellular polymeric substances around bacterial cells. Moreover, the functional gene annotation confirmed the presence of ATPase, ABC, and HoxN/HupN/NixA families of transporters. Thus, both the isolates provide a better solution for the removal of metal pollutants.


Assuntos
Metais Pesados , Ochrobactrum , Bioacumulação , Humanos , Metais Pesados/toxicidade , Anotação de Sequência Molecular , Ochrobactrum/genética
15.
Acta Diabetol ; 58(2): 191-195, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32940798

RESUMO

INTRODUCTION: The higher frequency of infections in diabetic patients is caused by a hyperglycemic environment, which promotes immune dysfunction. People with diabetes are more prone to skin infections. A continuous glucose monitoring (CGM) system provides information on changes in blood glucose (BG) levels throughout the day. Its use facilitates optimal therapeutic decisions for a diabetic patient. One of the factors limiting the use of CGM is inflammation at the insertion site. AIM OF THE STUDY: The aim of the study was the microbiological identification of the bacterial strains which are found on CGM sensor electrodes. MATERIAL AND METHODS: We performed microbiological tests on patients' CGM Enlite Medtronic electrodes, which were removed after 6 days of usage according to the manufacturer's instructions. 31 sensors were examined from 31 children (14 girls) aged from 0.5 to 14.6 years. The microbiological analysis was routinely performed at the Department of Children's Diabetology Medical University of Silesia in Katowice, Poland. RESULTS: 12 (39%) of the electrodes were colonized. In 11 (92%) cases the electrodes were colonized by one bacteria strain. 7 times methicillin-sensitive coagulase negative staphylococcus (MSCNS) was detected. We also found one case of Klebsiella pneumoniae, Ochrobactrum tritici, Bacillus sonorensis and methicillin-resistant coagulase-negative Staphylococci (MRCNS) colonization. One electrode was colonized by the mixed flora Enterococcus faecalis, methicillin-susceptible coagulase-negative Staphylococci (MSCNS), Pseudomonas stutzeri, methicillin-susceptible Staphylococcus aureus (MSSA). The median HbA1c in the group with colonization of electrodes was 6, 85% (6, 3-7, 6%) versus 6, 3% (5, 8-7, 5%) in the group without colonization. The median BMI in the group with colonization of the electrodes was 17.10 kg/m2 (16.28-18.62 kg/m2) versus 15.98 kg/m2 (15.14-17.96 kg/m2) in the group without colonization. Statistically, significantly more frequently electrodes are colonized in older children (median age in the group with colonization of electrodes 11.43 years (6.52-12.27 years), without colonization 8.42 years. (3.098-9.375 years); (p = 0.033). CONCLUSIONS: It seems that older children are more likely to have their sensor electrode colonized by bacterial strains.


Assuntos
Bactérias/isolamento & purificação , Glicemia/análise , Diabetes Mellitus Tipo 1/sangue , Contaminação de Equipamentos/estatística & dados numéricos , Equipamentos e Provisões/microbiologia , Adolescente , Bacillus/isolamento & purificação , Bactérias/classificação , Automonitorização da Glicemia/instrumentação , Automonitorização da Glicemia/estatística & dados numéricos , Criança , Pré-Escolar , Diabetes Mellitus Tipo 1/complicações , Diabetes Mellitus Tipo 1/epidemiologia , Diabetes Mellitus Tipo 1/microbiologia , Eletrodos/efeitos adversos , Eletrodos/microbiologia , Eletrodos/estatística & dados numéricos , Equipamentos e Provisões/efeitos adversos , Equipamentos e Provisões/normas , Equipamentos e Provisões/estatística & dados numéricos , Feminino , Humanos , Lactente , Masculino , Ochrobactrum/isolamento & purificação , Polônia/epidemiologia , Dermatopatias Bacterianas/complicações , Dermatopatias Bacterianas/epidemiologia , Dermatopatias Bacterianas/microbiologia , Staphylococcus/isolamento & purificação
16.
Int J Biol Macromol ; 166: 1046-1056, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-33157140

RESUMO

A newly isolated bacterium producing 55.5 U/mL keratinase on feather meal minimal medium was identified as Ochrobactrum intermedium. Optimization of process parameters by one-variable-at-a-time (OVAT) approach (substrate concentration 0.5% w/v, inoculum size 5% w/v, pH 7.0, 200 rpm for 96 h at 40 °C) resulted in 2.1-fold increase in keratinase secretion (117 U/mL). Keratinase was optimally active at pH 9.0 and 40 °C and was stable at pH 9.0 and 60 °C for 120 min. Calcium ions enhanced keratinase activity (158%) significantly, while it was strongly inhibited by both PMSF and EDTA, indicating it to be a metallo-serine protease. Keratinase degraded native chicken feathers efficiently resulting in 97.9% weight loss along with release of 745.5 µg/mL soluble proteins and 4196.69 µg/mL amino acids. Feather hydrolysate generated by NKIS 1 exhibited significant anti-oxidant and free-radical scavenging activity (90.46%). The present study revealed that O. intermedium NKIS 1 has potential applications in the biodegradation of chicken feathers and the value-addition of poultry waste.


Assuntos
Proteínas de Bactérias/metabolismo , Plumas/química , Ochrobactrum/enzimologia , Peptídeo Hidrolases/metabolismo , Animais , Proteínas Aviárias/metabolismo , Biodegradação Ambiental , Cálcio/metabolismo , Galinhas , Estabilidade Enzimática , Sequestradores de Radicais Livres/química , Resíduos Industriais , Queratinas/metabolismo , Hidrolisados de Proteína/química
17.
Folia Microbiol (Praha) ; 66(2): 189-196, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33131029

RESUMO

In the present study, bacterial isolates were screened for arsenic resistance efficiency. Environmental isolates were isolated from arsenic-rich soil samples (i.e., from Rajnandgaon district of Chhattisgarh state, India). Amplification and sequencing of 16S rRNA gene revealed that the isolates were of Bacillus firmus RSN1, Brevibacterium senegalense RSN2, Enterobacter cloacae RSN3, Stenotrophomonas pavanii RSN6, Achromobacter mucicolens RSN7, and Ochrobactrum intermedium RSN10. Arsenite efflux gene (arsB) was successfully amplified in E. cloacae RSN3. Atomic absorption spectroscopy (AAS) analysis showed an absorption of 32.22% arsenic by the RSN3 strain. Furthermore, results of scanning electron microscopy (SEM) for morphological variations revealed an initial increase in the cell size at 1 mM sodium arsenate; however, it was decreased at 10 mM concentration in comparison to control. This change of the cell size in different metal concentrations was due to the uptake and expulsion of the metal from the cell, which also confirmed the arsenite efflux system.


Assuntos
Arsênio , Poluentes do Solo , Achromobacter , Brevibacterium , Enterobacter cloacae/genética , Ochrobactrum , RNA Ribossômico 16S/genética , Solo , Stenotrophomonas
18.
Can J Microbiol ; 67(2): 138-146, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32841574

RESUMO

The SCUEC4 strain of Ochrobactrum intermedium is a newly isolated bacterium that degrades nicotine can use nicotine as the sole carbon source via a series of enzymatic catalytic processes. The mechanisms underlying nicotine degradation in this bacterium and the corresponding functional genes remain unclear. Here, we analyzed the function and biological properties of the ocnE gene involved in the nicotine-degradation pathways in strain SCUEC4. The ocnE gene was cloned by PCR with total DNA of strain SCUEC4 and used to construct the recombinant plasmid pET28a-ocnE. The overexpression of the OcnE protein was detected by SDS-PAGE analysis, and study of the function of this protein was spectrophotometrically carried out by monitoring the changes of 2,5-dihydroxypyridine. Moreover, the effects of temperature, pH, and metal ions on the biological activities of the OcnE protein were analyzed. The optimal conditions for the biological activities of OcnE, a protein of approximately 37.6 kDa, were determined to be 25 °C, pH 7.0, and 25 µmol/L Fe2+, and the suitable storage conditions for the OcnE protein were 0 °C and pH 7.0. In conclusion, the ocnE gene is responsible for the ability of 2,5-dihydroxypyridine dioxygenase. These findings will be beneficial in clarifying the mechanisms of nicotine degradation in O. intermedium SCUEC4.


Assuntos
Proteínas de Bactérias/metabolismo , Genes Bacterianos , Nicotina/metabolismo , Ochrobactrum/metabolismo , Proteínas de Bactérias/química , Proteínas de Bactérias/genética , Concentração de Íons de Hidrogênio , Ferro/metabolismo , Peso Molecular , Ochrobactrum/genética , Piridinas/metabolismo , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Temperatura
19.
Environ Monit Assess ; 192(11): 697, 2020 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-33043403

RESUMO

Environmental change is one of the primary issues faced by the farming community. Low rainfall and high temperature in arid and semiarid regions lead to the development of secondary salinisation, thus making the problem more severe. Under saline conditions, sodium is the most crucial cation that competes with potassium (K) and adversely affects plant metabolism by inhibiting plant enzymatic activities. Potassium-solubilising bacteria (KSB) play a vital role in solubilising fixed potassium and making it accessible to plants. In the current study, 42 KSB strains were isolated from paddy rhizosphere soil grown under salt-affected conditions. The plant-growth-promoting (PGP) properties of these rhizobacteria were also evaluated. Thirteen KSB strains, positive for all tested PGP traits, were evaluated for potassium solubilisation under sodium stress, namely, 0%, 3%, 5% and 7% NaCl stress. The five best strains (Acinetobacter pittii strain L1/4, A. pittii strain L3/3, Rhizobium pusense strain L3/4, Cupriavidus oxalaticus strain L4/12 and Ochrobactrum ciceri strain L5/1) based on the K-solubilising potential were identified by amplification, sequencing and bioinformatic analysis of the 16S rDNA sequences. The maximum potassium solubilisation was measured at 30 °C and pH 7 with glucose as carbon source. The application of these KSB strains significantly improved the shoot length, fresh weight, dry weight and chlorophyll contents of paddy plants grown under saline conditions. Hence, these strains could be halotolerant KSB bioinoculants that can be used to protect plants against salt stress.


Assuntos
Potássio , Microbiologia do Solo , Acinetobacter , Cupriavidus , Monitoramento Ambiental , Ochrobactrum , Rhizobium
20.
BMC Microbiol ; 20(1): 300, 2020 10 06.
Artigo em Inglês | MEDLINE | ID: mdl-33023493

RESUMO

BACKGROUND: Numerous studies have reported the health-promoting effects of exopolysaccharides (EPSs) in in vitro models; however, a functional evaluation of EPSs will provide additional knowledge of EPS-microbe interactions by in vivo intestinal microbial model. In the present study, high-throughput amplicon sequencing, short-chain fatty acid (SCFAs) and intestinal inflammation evaluation were performed to explore the potential benefits of exopolysaccharides (EPSs) and EPS-producing Lactobacillus (HNUB20 group) using the healthy zebrafish (Danio rerio) model. RESULTS: The results based on microbial taxonomic analysis revealed that the abundance of four genera, Ochrobactrum, Sediminibacterium, Sphingomonas and Sphingobium, were increased in the control group in comparison to HNUB20 group. Pelomonas spp. levels were significantly higher and that of the genera Lactobacillus and Brachybacterium were significantly decreased in EPS group compared with control group. PICRUSt based functional prediction of gut microbiota metabolic pathways indicated that significantly lower abundance was found for transcription, and membrane transport, whereas folding, sorting and degradation and energy metabolism had significantly higher abundance after HNUB20 treatment. Two metabolic pathways, including metabolism and endocrine functions, were more abundant in the EPS group than control group. Similar to the HNUB20 group, transcription was also decreased in the EPS group compared with the control group. However, SCFAs and immune indexes indicated EPS and HNUB20 performed limited efficacy in the healthy zebrafish. CONCLUSIONS: The present intestinal microbial model-based study indicated that EPSs and high-yield EPS-producing Lactobacillus can shake the structure of intestinal microbiota, but cannot change SCFAs presence and intestinal inflammation.


Assuntos
Microbioma Gastrointestinal/fisiologia , Intestinos/microbiologia , Lactobacillus/fisiologia , Polissacarídeos Bacterianos/farmacologia , Peixe-Zebra/microbiologia , Actinobacteria/fisiologia , Animais , Bacteroidetes/fisiologia , Comamonadaceae/fisiologia , Ácidos Graxos Voláteis/metabolismo , Feminino , Masculino , Redes e Vias Metabólicas/genética , Ochrobactrum/fisiologia , Polissacarídeos Bacterianos/biossíntese , Sphingomonadaceae/fisiologia , Sphingomonas/fisiologia , Transcrição Genética
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...