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1.
Biomater Sci ; 10(9): 2409-2416, 2022 May 04.
Artigo em Inglês | MEDLINE | ID: mdl-35384952

RESUMO

Infected wound healing is a complex and dynamic process affecting millions of people. Since wound healing contains multiple stages, it requires staged management to realize the early inhibition of infection and the subsequent promotion of wound healing. A key point is to design a biphasic release system with antibacterial agents and growth factors to promote wound regeneration. As a safe, efficient and painless transdermal drug delivery method, microneedles (MNs) have attracted widespread attention. Herein, we present dissolving MNs with the biphasic release of an antibacterial agent and a growth factor to promote wound healing. bFGF was first encapsulated in PLGA microspheres (bFGF@PLGA) and then co-loaded with free ofloxacin onto polyvinylpyrrolidone MNs. Owing to the fast dissolution of the substrate, ofloxacin was quickly released to rapidly inhibit infection, while the PLGA microspheres were left in the wound. Due to the slow degradation of PLGA, bFGF encapsulated in the PLGA microspheres was slowly released to further promote wound healing. In vivo studies demonstrated that the MNs with the biphasic release of antibacterial agent and growth factor exhibited a superior capability to promote wound healing. This biphasic release system combined with microneedles has a bright future in wound healing.


Assuntos
Antibacterianos , Cicatrização , Antibacterianos/farmacologia , Humanos , Peptídeos e Proteínas de Sinalização Intercelular , Microesferas , Ofloxacino
2.
Anal Bioanal Chem ; 414(12): 3687-3696, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35318515

RESUMO

Using a solid-phase molecular imprinting technique, high-affinity nanoparticles (nanoMIPs) selective for the target antibiotics, ciprofloxacin, moxifloxacin, and ofloxacin have been synthesised. These have been applied in the development of a surface plasmon resonance (SPR) sensor for the detection of the three antibiotics in both river water and milk. The particles produced demonstrated good uniformity with approximate sizes of 65.8 ± 1.8 nm, 76.3 ± 4.1 nm, and 85.7 ± 2.5 nm, and were demonstrated to have affinities of 36.2 nM, 54.7 nM, and 34.6 nM for the ciprofloxacin, moxifloxacin, and ofloxacin nanoMIPs, respectively. Cross-reactivity studies highlighted good selectivity towards the target antibiotic compared with a non-target antibiotic. Using spiked milk and river water samples, the nanoMIP-based SPR sensor offered comparable affinity with 66.8 nM, 33.4 nM, and 55.0 nM (milk) and 39.3 nM, 26.1 nM, and 42.7 nM (river water) for ciprofloxacin, moxifloxacin, and ofloxacin nanoMIPs, respectively, to that seen within a buffer standard. Estimated LODs for the three antibiotic targets in both milk and river water were low nM or below. The developed SPR sensor showed good potential for using the technology for the capture and detection of antibiotics from food and environmental samples.


Assuntos
Impressão Molecular , Nanopartículas , Alérgenos , Animais , Antibacterianos , Ciprofloxacina , Leite , Polímeros Molecularmente Impressos , Moxifloxacina , Ofloxacino , Rios , Ressonância de Plasmônio de Superfície , Água
3.
Chemosphere ; 298: 134243, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35278448

RESUMO

Hospital wastewater is harmful to the environment and human health due to its complex chemical composition and high potency towards becoming a source of disease outbreaks. Due to these complexities, its treatment is neither efficient nor cost-effective. It is a challenging issue that requires immediate attention. This effort focuses on the treatment of hospital wastewater (HWW) by removing two selected drugs, namely ibuprofen (IBU) and ofloxacin (OFX) using individual biological treatment methods, such as moving bed biofilm reactors (MBBR) and physicochemical treatment, such as ozonation and peroxane process. The both methods are compared to find the best method overall based on effectiveness and removal efficiency. The optimal removal for ozone dosing range was nitrate (9.00% and 62.00%), biological oxygen demand (BOD) (92.00% and 64.00%), and chemical oxygen demand (COD) (96.00% and 92.00%) that required at least 10 min to reach considerable degradation. The MBBR process assured a better performance for ibuprofen removal, overall. The IBU and OFX removal was found to be 14.32-96.00% at a higher COD value and 11.33-94.00% at a lower COD value due to its biodegradation. This work strives to pave the way forward to build an HWW treatment technology using integrated MBBR processes for better efficiency and cost-effectiveness.


Assuntos
Poluentes Químicos da Água , Purificação da Água , Biofilmes , Reatores Biológicos , Hospitais , Humanos , Ibuprofeno , Ofloxacino , Preparações Farmacêuticas , Eliminação de Resíduos Líquidos/métodos , Águas Residuárias/química , Poluentes Químicos da Água/análise
4.
Microbiol Spectr ; 10(2): e0251621, 2022 Apr 27.
Artigo em Inglês | MEDLINE | ID: mdl-35311541

RESUMO

Our objective was to evaluate the performance of whole-genome sequencing (WGS) from early positive liquid cultures for predicting Mycobacterium tuberculosis complex (MTBC) drug resistance. Clinical isolates were obtained from tuberculosis patients at Shanghai Pulmonary Hospital (SPH). Antimicrobial susceptibility testing (AST) was performed, and WGS from early Bactec mycobacterial growth indicator tube (MGIT) 960-positive liquid cultures was performed to predict the drug resistance using the TB-Profiler informatics platform. A total of 182 clinical isolates were enrolled in this study. Using phenotypic AST as the gold standard, the overall sensitivity and specificity for WGS were, respectively, 97.1% (89.8 to 99.6%) and 90.4% (83.4 to 95.1%) for rifampin, 91.0% (82.4 to 96.3%) and 95.2% (89.1 to 98.4%) for isoniazid, 100.0% (89.4 to 100.0%) and 87.3% (80.8 to 92.1%) for ethambutol, 96.6% (88.3 to 99.6%) and 61.8% (52.6 to 70.4%) for streptomycin, 86.8% (71.9 to 95.6%) and 95.8% (91.2 to 98.5%) for moxifloxacin, 86.5% (71.2 to 91.5%) and 95.2% (90.3 to 98.0%) for ofloxacin, 100.0% (54.1 to 100.0%) and 67.6% (60.2 to 74.5%) for amikacin, 100.0% (63.1 to 100.0%) and 67.2% (59.7 to 74.2%) for kanamycin, 62.5% (24.5 to 91.5%) and 88.5% (82.8 to 92.8%) for ethionamide, 33.3% (4.3 to 77.7%) and 98.3% (95.1 to 99.7%) for para-aminosalicylic acid, and 0.0% (0.0 to 12.3%) and 100.0% (97.6 to 100.0%) for cycloserine. The concordances of WGS-based AST and phenotypic AST were as follows: rifampin (92.9%), isoniazid (93.4%), ethambutol (89.6%), streptomycin (73.1%), moxifloxacin (94.0%), ofloxacin (93.4%), amikacin (68.7%), kanamycin (68.7%), ethionamide (87.4%), para-aminosalicylic acid (96.2%) and cycloserine (84.6%). We conclude that WGS could be a promising approach to predict MTBC resistance from early positive liquid cultures. IMPORTANCE In this study, we used whole-genome sequencing (WGS) from early positive liquid (MGIT) cultures instead of solid cultures to predict drug resistance of 182 Mycobacterium tuberculosis complex (MTBC) clinical isolates to predict drug resistance using the TB-Profiler informatics platform. Our study indicates that WGS may be a promising method for predicting MTBC resistance using early positive liquid cultures.


Assuntos
Ácido Aminossalicílico , Mycobacterium tuberculosis , Tuberculose Resistente a Múltiplos Medicamentos , Amicacina , Antituberculosos/farmacologia , Antituberculosos/uso terapêutico , China , Ciclosserina , Resistência a Medicamentos , Etambutol , Etionamida , Humanos , Isoniazida , Canamicina , Testes de Sensibilidade Microbiana , Moxifloxacina , Ofloxacino , Rifampina , Estreptomicina , Tuberculose Resistente a Múltiplos Medicamentos/diagnóstico , Tuberculose Resistente a Múltiplos Medicamentos/tratamento farmacológico , Tuberculose Resistente a Múltiplos Medicamentos/microbiologia
5.
Spectrochim Acta A Mol Biomol Spectrosc ; 273: 121066, 2022 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-35231759

RESUMO

Four simple, precise, accurate and validated spectrophotometric methods have been developed for the simultaneous determination of ofloxacin (OFL) and bromfenac sodium (BROM). Firstly, first and second derivative spectrophotometric methods (1D &2D) using a zero-crossing technique utilizing 309.3 and 257.5 nm for OFL and 290.7 and 246.5 nm for BROM as optimum working wavelengths in a binary mixture, respectively. Secondly, the first derivative ratio spectrophotometric method (1DD) in which peak amplitudes at 297.3 nm and 260.7 nm were chosen to simultaneously estimate OFL and BROM, respectively. Thirdly, dual wavelength (DW) method based on two selected wavelengths for each drug in such a way that the difference in absorbance is zero for the second one. At wavelengths 296.4, 348.4 nm BROM has equal absorbance values, therefore, these two wavelengths have been used to determine OFL. Similarly, 271.7 nm and 313.1 nm were selected to determine BROM in the combined formulation. Finally, the fourth method depends on ratio difference spectrophotometry (RDSM), in which the difference between amplitudes at 305.6 nm and 326.5 nm on the ratio spectrum of the mixture was directly proportional to the concentration of OFL; independent of the interfering components. Similarly, the difference between amplitudes at 265.1 nm and 275.4 nm on the ratio spectrum was used for the determination of BROM. The linearity was confirmed in the range of 4 - 18 µg/ml for OFL and BROM for the four methods. The proposed methods were used to determine both drugs in their laboratory prepared mixture and combined formulation with mean percentage recoveries of 99.41 ± 1.35% for OFL and 99.98 ± 1.30 % for BROM in method (A). In method (B), the mean percentage recoveries were 101.70 ± 1.61% for OFL and 101.90 ± 1.45% for BROM. In method (C) OFL was 99.57 ± 1.61% and 100.90 ± 1.62% for BROM. Finally, in method (D) the mean percentage recoveries were 99.37 ± 1.67% for OFL and 100.70 ± 1.59% for BROM. The developed methods were successfully employed for determination of OFL and BROM in laboratory prepared mixtures and combined formulation showing satisfactory recoveries. Methods validation was performed according to the International Conference on Harmonization (ICH) guidelines. The obtained results conformed to the accepted ranges of recovery, precision and repeatability.


Assuntos
Bromobenzenos , Ofloxacino , Benzofenonas , Espectrofotometria/métodos
6.
Bioresour Technol ; 350: 126938, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35247560

RESUMO

Solid-phase denitrification has been applied for advanced nitrogen removal from wastewater and can co-degrade emerging pollutants. Fluoroquinolones (FQs), broad-spectral antibiotic, are frequently detected in the effluent of conventional wastewater treatment plants. However, it remains unclear whether solid-phase denitrifying bacteria can remove FQs. Thus, this study investigated the removal capacity of ofloxacin (OFX) as a representative of FQs and the microbial community structures of denitrifying sludge acclimated to polycaprolactone and OFX. The Results indicate that OFX had a negative effect on denitrification performance. OFX was degraded, and a possible pathway was revealed based on ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry. The dominant genera in the acclimated denitrifying sludge were Microbacterium, Simplicispira, Alicycliphilus, Reyranella, Sediminibacterium, Acidovorax and Thermomonas. Moreover, ABC transporters and cytochrome P450, related to multi-drug resistance and drug metabolism, were highly expressed in the acclimated sludge. This study provides novel insights into antibiotics control.


Assuntos
Comamonadaceae , Desnitrificação , Reatores Biológicos/microbiologia , Carbono/química , Nitrogênio/química , Ofloxacino , Poliésteres , Esgotos/microbiologia , Águas Residuárias/química
7.
J Hazard Mater ; 431: 128462, 2022 06 05.
Artigo em Inglês | MEDLINE | ID: mdl-35220123

RESUMO

Graphene sponge electrodes doped with atomic boron and nitrogen were employed for electrochemical degradation of antibiotics sulfamethoxazole, trimethoprim, ofloxacin, and erythromycin. The removal of antibiotics that displayed strong π-π interactions (i.e., ofloxacin) with reduced graphene oxide (RGO) coating was less limited by the mass transfer and removal efficiencies > 80% were observed for the investigated range of electrolyte flowrates. At the highest applied flowrate (700 LMH), increase in the anodic current significantly worsened the removal of trimethoprim and erythromycin due to the detrimental impact of the evolving gas bubbles. Increase in current at 700 LMH led to a stepwise increase in the removal efficiency of sulfamethoxazole due to its enhanced electrosorption. Electrochemical degradation was achieved via ozone, hydrogen peroxide and hydroxyl radical (•OH). Extraction of the employed graphene sponges confirmed the degradation of the strongly adsorbing antibiotics. Identified electrochemical transformation products of erythromycin confirmed the participation of •OH, through N-demethylation of the dimethylamine group. In real tap water, removal efficiencies were lower for all target antibiotics. Lower electric conductivity of tap water and thus increased thickness of the electric double layer likely limited their interaction with the graphene sponge surface, in addition to the presence of low amounts of organic matter.


Assuntos
Grafite , Poluentes Químicos da Água , Antibacterianos , Eletrodos , Eritromicina , Ofloxacino , Oxirredução , Sulfametoxazol , Trimetoprima , Água , Poluentes Químicos da Água/análise
8.
Environ Res ; 210: 112937, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35157918

RESUMO

The high-throughput production of the eco-friendly MIL-88A(Fe) was achieved under mild reaction conditions with normal pressure and temperature. The as-prepared MIL-88A(Fe) exhibited efficient photo-Fenton catalytic ofloxacin (OFL) degradation upon visible light irradiation with good stability and reusability. The OFL (20.0 mg/L) was completely degraded within 50 min under visible light with the aid of MIL-88A(Fe) (0.25 g/L) and H2O2 (1.0 mL/L) in aqueous solution (pH = 7.0). The hydroxyl radicals (·OH) are the main active species during the photo-Fenton oxidation process. Meanwhile, the degradation intermediates and the corresponding degradation pathways were identified and proposed with the aid of both ultra-high performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) and density functional theory (DFT) calculations. Finally, the degradation product library was firstly established to identify intermediate transformation products (TPs) with their variation of concentration, and their corresponding toxicologic activities were assessed via Toxtree and T.E.S.T software as well. Finally, the MIL-88A is efficient and stable with four cycles' catalysis operations, demonstrating good potential for water treatment.


Assuntos
Ofloxacino , Purificação da Água , Catálise , Peróxido de Hidrogênio/química , Dietilamida do Ácido Lisérgico/análogos & derivados , Ofloxacino/toxicidade , Purificação da Água/métodos
9.
J Colloid Interface Sci ; 615: 650-662, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35158196

RESUMO

In this work, Co3O4/SnO2 catalyst was prepared by a one-pot hydrothermal method and applied in the activation of peroxymonosulfate (PMS) for the degradation of the target pollutant ofloxacin (OFX). The results showed that the PMS/Co3O4/SnO2-8% system had a 92% OFX degradation efficiency after 30 min of catalytic reaction, which was 46 times higher than that of PMS/SnO2 alone, and the degradation efficiency could be maintained in a wide pH range (5-11). In addition, reactive oxygen species quenching experiments and electron spin resonance spectra confirmed that sulfate radicals, superoxide radicals, hydroxyl radicals and singlet oxygen were the dominant active groups. The excellent recyclability and stability of the as-prepared catalyst were confirmed by cycling experiments and characterization results. Finally, a possible degradation pathway of OFX was suggested, and the intermediate toxicity of this system was identified and analyzed by a quantitative structure-activity relationship (QSAR).


Assuntos
Ofloxacino , Peróxidos , Cobalto , Luz , Ofloxacino/farmacologia , Óxidos , Peróxidos/química
10.
Sci Total Environ ; 823: 153765, 2022 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-35157859

RESUMO

Drug chirality is attracting increasing attention because the enantiomers of the same chiral pharmaceutical usually exhibit different biological activities, metabolic pathways, and toxicities. The ubiquitous presence of microplastics (MPs) can enrich organic pollutants commonly found in the environment. However, knowledge about the enrichment of pharmaceutical enantiomers to MPs is relatively limited. We investigated the occurrence of enantioselectivity of ofloxacin (OFL) and levofloxacin (LEV) in the adsorption processes on polyethylene (PE) and the interactions influenced by environmental factors. The results showed that the adsorption efficiency of OFL was generally 3-5% (p < 0.05) higher than that of LEV, indicating the different affinities of the enantiomers to PE, but the adsorption process of OFL and LEV on PE was both well described by pseudo-first-order kinetics and liner isotherm models. The chirality of OFL and LEV was not affected by sizes of PE particles and solution salinity due to the identical physicochemical properties. An examination of pH effect indicated that OFL showed better acid-base adaptability than LEV. Moreover, the differences in enantiomeric enrichment between OFL and LEV on PE were promoted with increasing UV light exposure time and natural organic matter (NOM) concentrations. Using Fourier transform infrared spectroscopy (FTIR), we demonstrated that the constituents of the functional groups in chiral NOM were greatly related to the enantiomer stereoselectivity of OFL, subsequently affecting their adsorption in a chiral environment. The excitation-emission matrix (EEM) spectra confirmed the enantioselective behaviors of chiral pharmaceuticals under UV light due to the different optical activity and humic acid-like and fulvic acid-like molecular structure of the enantiomers. These findings imply that the enantioselectivity of drug enantiomers should be considered in presence of microplastics, leading to a more accurate environmental fate and risks assessments of chiral pharmaceuticals.


Assuntos
Microplásticos , Poluentes Químicos da Água , Adsorção , Levofloxacino , Ofloxacino/análise , Preparações Farmacêuticas , Plásticos , Polietileno , Poluentes Químicos da Água/análise
11.
Sci Rep ; 12(1): 2296, 2022 02 10.
Artigo em Inglês | MEDLINE | ID: mdl-35145139

RESUMO

This study aimed to assess the presence of qnrA, qnrB, qnrC, qnrD, qnrS, qepA, and aac(6')-Ib-cr determinants as well as quinolone resistance pattern of clinical isolates of P. aeruginosa in Ahvaz, southwest Iran. A total of 185 clinical isolates of P. aeruginosa were collected from 5 university-affiliated hospitals in Ahvaz, southwest Iran. The disk diffusion method was applied to assess the quinolone resistance pattern. The presence of qnrA, qnrB, qnrC, qnrD, qnrS, qepA, and aac(6')-Ib-cr genes was investigated by the polymerase chain reaction (PCR) method. Overall, 120 (64.9%) isolates were non-susceptible to quinolones. The most and the less quinolone resistance rates were observed against ciprofloxacin (59.4%) and ofloxacin (45.9%), respectively. The prevalence rates of qnr genes were as follows: qnrA (25.8%), qnrB (29.2%), and qnrS (20.8%). The qnrB gene was the most common type of qnr genes. The qnr genes were occurred in 37.5% (n = 45/120) of quinolne-resistant isolates, simultaneously. The qnrC, qnrD, qepA, and aac(6')-Ib-cr genes were not recognized in any isolates. In conclusion, the ofloxacin was the most effective quinolone. This study was the first to shed light on the prevalence of PMQR genes among P. aeruginosa isolates in southwest Iran.


Assuntos
Antibacterianos/farmacologia , Farmacorresistência Bacteriana/genética , Genes Bacterianos/genética , Pseudomonas aeruginosa/efeitos dos fármacos , Pseudomonas aeruginosa/genética , Quinolonas/farmacologia , Ciprofloxacina/farmacologia , Humanos , Irã (Geográfico) , Testes de Sensibilidade Microbiana/métodos , Ofloxacino/farmacologia , Pseudomonas aeruginosa/isolamento & purificação
12.
Chemosphere ; 295: 133784, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35114255

RESUMO

Photocatalysis technology exhibited promising application for advanced treatment of wastewater. Nevertheless, the design of efficient photocatalyst and the mechanism of free radicals in pollutant degradation still remained to be further investigated. Herein, BiVO4/CQDs/ß-FeOOH photocatalyst was fabricated by electrostatic self-assembly method, which exhibited the excellent photocatalytic performance. Under visible-light irradiation, the removal rate of ofloxacin by BiVO4/CQDs/ß-FeOOH (0.25 min-1) was 1.93 times than pristine BiVO4, and the removal efficiency in 15 min reached 99.21%. The perfect reusability of BiVO4/CQDs/ß-FeOOH was ascribed to the persistent catalytic active centers provided by the renewable surface oxygen vacancies on the ß-FeOOH. As electron transfer channels, CQDs facilitated the transfer of BiVO4 photogeneration electrons. The matched band structure allowed the construction of S-scheme heterojunctions, and the higher conduction band position was retained while the carrier separation was promoted. More importantly, this work firstly reported the phenomenon that the main reactive groups in the photocatalysis process would be directionally transformed with the change of pH conditions. Based on the analysis of capture and electron paramagnetic resonance experiments, ·O2- was the main free radicals to photodegrade OFL in neutral and alkaline conditions. However, when the solution pH turned into acidic, the photodegradation of OFL was dominated by 1O2. This innovative phenomenon was due to that acidic condition accelerated the reaction kinetics of spontaneous transformation of ·O2- to 1O2 and inhibited the direct oxidation of pollutants by ·O2-. Accordingly, this research could inspire theoretical study of free radical reaction and the design of S-scheme heterojunction photocatalysts.


Assuntos
Ofloxacino , Catálise , Compostos Férricos , Fotólise , Espécies Reativas de Oxigênio
13.
Aquat Toxicol ; 244: 106084, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-35078055

RESUMO

Ofloxacin (OFL) is a broad-spectrum fluoroquinolone antibiotic frequently used in clinic for treating bacterial infections. The discharged OFL would inevitably enter into aquatic ecosystems, affecting the growth of non-target microorganisms, which may result in micro-ecosystem imbalance. To the best of our knowledge, researches in this area are rather sparse. The present study evaluated the response of photosynthetic microorganisms (cyanobacteria, eukaryotic algae) and aquatic microbial community to OFL in a microcosm. Results showed that ofloxacin presented an inhibitory effect on the growth Microcystis aeruginosa. Although 0.1 mg/L OFL has no significant impact on alpha diversity of the microbial communities, it obviously altered the structure and decreased the species interaction of prokaryotic community by reducing the capacities of nitrogen fixation, photosynthetic and metabolic capacity of the microbial community. This study pointed out that the residual OFL in water would disturb the balance of the aquatic micro-ecology, suggesting that more attentions should be given to the negative effects of antibiotics and other bioactive pollutants on aquatic environments.


Assuntos
Microbiota , Microcystis , Poluentes Químicos da Água , Água Doce , Ofloxacino/toxicidade , Poluentes Químicos da Água/toxicidade
14.
Chemosphere ; 294: 133743, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35093424

RESUMO

In Fenton or Fenton-like processes, the key step is to catalyze H2O2 and produce highly reactive OH radicals. More efforts are then focus on designing efficient heterogeneous Fenton catalysts by activating H2O2 to generate OH at the highest possible steady state concentration. In this study, using the antibiotic ofloxacin as target organic pollutant, we firstly demonstrate a point of view for improving OH utilization efficiency by regulating surface chemical reactions to minimizing its migration distance to the target pollutant. C doped g-C3N4 incorporated CuFeO2 (CCN/CuFeO2) exhibited almost ten times higher ofloxacin degradation rate constant than our previously reported CuFeO2 {012} catalyst (0.1634 vs 0.0179 min-1). Since similar amount of OH was generated, the different inhibition effect of tert-butyl alcohol and nitrobenzene on the ofloxacin degradation confirmed that the much-enhanced ofloxacin degradation was attributed to the surface Fenton reaction process. According to XPS and EXAFS characterization, the C-O-Cu bond between g-C3N4 and CuFeO2 established a closed-circuit surface Fenton reaction mechanism. H2O2 was adsorbed and decomposed into OH/O2- over ≡Cu + site in CuFeO2. The successful construction of CCN/CuFeO2 creates a negative surface potential and benefits the enrichment of target antibiotics from water, which greatly reduces the migration distance of OH/O2•- to adjacent pollutant and then increases the OH/O2- utilization efficiency by avoiding the unwanted quenching. Hence, CCN/CuFeO2 possesses superior Fenton catalytic activity and long-term stability.


Assuntos
Peróxido de Hidrogênio , Nanocompostos , Antibacterianos , Catálise , Ofloxacino
15.
Int J Infect Dis ; 116: 355-357, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-35017111

RESUMO

In November 2021, a 19-year-old medical student was diagnosed with a rabbit-derived Thelazia callipaeda infection in her left eye. She visited her doctor because she removed the white object by herself with a cotton swab 2 days earlier. She explained to the doctor that she rubbed her eyes without washing her hands when rabbit hair got into her eyes during the animal experiment class at school 2 weeks earlier. The other 2 worms were then taken out with ophthalmic forceps and were placed into physiological saline. The worms were sent to the Shandong Academy of Eye Disease Prevention and Therapy for examination. After identification, the worms were regarded as the female T. callipaeda. Ofloxacin eye drops and peranapulin eye drops were administered to control inflammation. No abnormality was found in the follow-up 2 weeks and 1 month later. This report highlights that it is very important to strengthen the health and safety education of medical students during animal experiment classes.


Assuntos
Oftalmopatias , Infecções por Spirurida , Thelazioidea , Animais , Feminino , Humanos , Ofloxacino , Coelhos , Infecções por Spirurida/diagnóstico , Infecções por Spirurida/tratamento farmacológico , Thelazioidea/fisiologia
16.
Braz J Otorhinolaryngol ; 88(1): 9-14, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-32456874

RESUMO

INTRODUCTION: Traumatic large tympanic membrane perforations usually fail to heal and require longer healing times. Few studies have compared the healing and hearing outcomes between gelatin sponge patching and ofloxacin otic solution. OBJECTIVES: To compare the healing outcomes of large traumatic tympanic membrane perforations treated with gelatin sponge, ofloxacin otic solution, and spontaneous healing. METHODS: Traumatic tympanic membrane perforations >50% of the entire eardrum were randomly divided into three groups: ofloxacin otic solution, gelatin sponge patch and spontaneous healing groups. The healing outcome and hearing gain were compared between the three groups at 6 months. RESULTS: A total of 136 patients with large traumatic tympanic membrane perforations were included in analyses. The closure rates were 97.6% (40/41), 87.2% (41/47), and 79.2% (38/48) in the ofloxacin otic solution, gelatin sponge patch, and spontaneous healing groups, respectively (p=0.041). The mean times to closure were 13.12±4.61, 16.47±6.24, and 49.51±18.22 days in these groups, respectively (p<0.001). CONCLUSIONS: Gelatin sponge patch and ofloxacin otic solution may serve as effective and inexpensive treatment strategies for traumatic large tympanic membrane perforations. However, ofloxacin otic solution must be self-applied daily to keep the perforation edge moist, while gelatin sponge patching requires periodic removal and re-patching.


Assuntos
Gelatina , Perfuração da Membrana Timpânica , Humanos , Ofloxacino , Membrana Timpânica , Perfuração da Membrana Timpânica/tratamento farmacológico , Cicatrização
17.
Homeopathy ; 111(1): 42-48, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-34020481

RESUMO

BACKGROUND: This study presents the results of the minimum inhibitory concentration (MIC) assay of a series of nosodes: namely Escherichia coli, Klebsiella pneumoniae, Salmonella typhi, Neisseria gonorrhoeae, and Candida albicans. Each was tested against its corresponding infection as well as cross infections. METHODS: In-vitro efficacy of polyvalent nosodes was tested using the MIC assay technique. The nosodes, namely C. albicans polyvalent nosode (35c, 100c), N. gonorrhoeae (35c), K. pneumoniae (35c, 100c), E. coli polyvalent nosode (35c, 100c) and Salmonella typhi polyvalent nosode (30c, 100c), were tested along with positive and negative controls. Nosodes were studied in different potencies and at 1:1 dilution. RESULTS: C. albicans polyvalent nosode 35c, 100c, N. gonorrhoeae 35c, and positive control amphotericin B showed inhibition of the growth of C. albicans species. K. pneumoniae 35c, E. coli polyvalent nosode 100c, and meropenem (positive control) showed inhibition of the growth of K. pneumoniae; this effect was not seen with ceftriaxone, ofloxacin and amoxicillin antibiotics. E. coli polyvalent nosode 30c in 10% alcohol (direct and dilution 1:1) and the positive controls ciprofloxacin, ofloxacin, and amoxicillin showed inhibition of the growth of E. coli. The S. typhi polyvalent nosode 30c in 10% alcohol showed inhibition of growth of S. typhi. CONCLUSION: This study reveals that the tested nosodes exhibited antibacterial potential against the corresponding micro-organisms and against other selected organisms studied using this assay.


Assuntos
Homeopatia , Materia Medica , Amoxicilina/farmacologia , Antibacterianos/farmacologia , Candida albicans , Escherichia coli , Klebsiella pneumoniae , Materia Medica/farmacologia , Testes de Sensibilidade Microbiana , Neisseria gonorrhoeae , Ofloxacino/farmacologia , Salmonella typhi
18.
Dig Dis Sci ; 67(1): 208-215, 2022 01.
Artigo em Inglês | MEDLINE | ID: mdl-33559090

RESUMO

AIMS: Quinolone-containing triple therapy has been considered as the second-line therapy for eradication of Helicobacter pylori (H. pylori). At present, there are no data to show the efficacy and safety of antofloxacin-based rescue therapy for the eradication of H. pylori, and this pilot clinical trial was designed. METHODS: A total of 196 patients who failed H. pylori eradication using the clarithromycin-based or metronidazole-based triple or bismuth quadruple therapy were randomly allocated to one of the following rescue eradication therapy groups: AEA group (antofloxacin 200 mg once daily, esomeprazole 20 mg + amoxicillin 1000 mg twice daily) for 14 days, or LEA group (levofloxacin 500 mg once daily, esomeprazole 20 mg + amoxicillin 1000 mg twice daily) for 14 days. The minimal inhibitory concentrations were tested by the E-test method. The gyrA mutation was analyzed by sequencing. Follow-up 13/14C-urea breath test was examined at 1 month after discontinuation. RESULTS: A total of 178 eligible patients were included in this study. The eradication rate was significantly higher in AEA group than in LEA group according to both ITT (87.6% vs. 68.5%; P = 0.002) and PP analyses (90.7% vs. 70.1%; P = 0.001). ITT analyses indicated that the eradication rate was significantly higher in AEA group than in LEA group with Asn87 mutation (78.9% vs. 31.3%; P = 0.005) and levofloxacin-resistant strains (76.9% vs. 44.2%; P = 0.003). Two groups exhibited similar adverse event rates (AEA 14.6% vs. LEA 20.2%, P = 0.323). CONCLUSIONS: The findings showed that antofloxacin may be a promising candidate in rescue therapy for H. pylori eradication failure in China.


Assuntos
Amoxicilina/administração & dosagem , Esomeprazol/administração & dosagem , Gastrite , Infecções por Helicobacter , Levofloxacino/administração & dosagem , Ofloxacino/análogos & derivados , Adulto , Antibacterianos/administração & dosagem , Testes Respiratórios/métodos , China , Quimioterapia Combinada , Feminino , Gastrite/tratamento farmacológico , Gastrite/microbiologia , Infecções por Helicobacter/diagnóstico , Infecções por Helicobacter/tratamento farmacológico , Helicobacter pylori/efeitos dos fármacos , Helicobacter pylori/isolamento & purificação , Humanos , Masculino , Testes de Sensibilidade Microbiana/métodos , Ofloxacino/administração & dosagem , Inibidores da Bomba de Prótons/administração & dosagem , Resultado do Tratamento
19.
Appl Environ Microbiol ; 88(3): e0232721, 2022 02 08.
Artigo em Inglês | MEDLINE | ID: mdl-34936843

RESUMO

New antimicrobial agents are urgently needed to combat the emergence and spread of multidrug-resistant bacteria. Activating the cryptic biosynthetic gene clusters for actinomycete secondary metabolites can provide essential clues for research into new antimicrobial agents. An effective method for this purpose is based on drug resistance selection. This report describes interesting results for drug resistance selection using antibiotics that target DNA replication and can effectively potentiate secondary metabolite production by actinomycetes. Ofloxacin-resistant mutants were isolated from five different streptomycetes. Ofloxacin is an antibiotic that binds to DNA complexes and type II topoisomerase, causing double-stranded breaks in bacterial chromosomes. Physiological and genetic characterization of the mutants revealed that the development of ofloxacin resistance in streptomycetes leads to the emergence of various types of secondary metabolite-overproducing strains. In Streptomyces coelicolor A3(2), ofloxacin-resistant mutants that overproduced actinorhodin, undecylprodigiosin, or carotenoid were identified. An ofloxacin-resistant mutant that overproduces methylenomycin A, whose biosynthetic gene cluster is located on the endogenous plasmid, SCP1, also was isolated. These observations indicate that ofloxacin resistance activates biosynthetic genes on both chromosomes and endogenous plasmids. We also identified the mutations that are probably involved in the phenotype of ofloxacin resistance and secondary metabolite overproduction in S. coelicolor A3(2). Furthermore, we observed an interesting phenomenon in which several ofloxacin-resistant mutants overproduced antibiotics in the presence of ofloxacin. Based on these results, we present the unique physiological and genetic characteristics of ofloxacin-resistant Streptomyces mutants and discuss the importance and potential development of the new findings. IMPORTANCE The abuse or overuse of antibacterial agents for therapy and animal husbandry has caused an increased population of antimicrobial-resistant bacteria in the environment. Consequently, fewer effective antimicrobials are now available. Due to the depleted antibiotic pipeline, pandemic outbreaks caused by antimicrobial-resistant bacteria are deeply concerning, and the development of new antibiotics is now an urgent issue. Promising sources of antimicrobial agents include cryptic biosynthetic gene clusters for secondary metabolites in streptomycetes and rare actinomycetes. This study's significance is the development of an unprecedented activation method to accelerate drug discovery research on a global scale. The technique developed in this study could allow for simultaneous drug discovery in different countries, maximizing the world's microbial resources.


Assuntos
Streptomyces coelicolor , Streptomyces , Antibacterianos/metabolismo , Família Multigênica , Ofloxacino/farmacologia , Streptomyces/genética , Streptomyces/metabolismo , Streptomyces coelicolor/genética , Streptomyces coelicolor/metabolismo
20.
Sci Total Environ ; 806(Pt 4): 150968, 2022 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-34656585

RESUMO

Cellulose and lignin derived biochars with significant differences in persistent free radicals (PFRs), oxygen-containing functional groups, and defective structure were prepared to explore the mechanism of biochar mediated persulfate (PS) activation. EPR spin trapping and quenching technique coupled with degradation experiments confirmed that the defective structures could activate PS to generate superoxide anions (O2•-), which was converted to singlet oxygen (1O2), especially in the acidic condition. 1O2 dominated the degradation of ofloxacin (OFL, a fluoroquinolone antibiotic). An improved iodometric measurement was applied for direct quantification of adsorbed PS on biochar. The amounts of adsorbed PS were consistent with the degradation of OFL and the measured electric current during the reaction indicated that PS adsorption was a prerequisite for PS activation, which may be neglected in previous studies. The results of this study highlighted the key roles of defective structure and adsorption of PS on biochar for the activation of PS.


Assuntos
Carbono , Ofloxacino , Adsorção , Carvão Vegetal
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